ISRN Parasitology

Volume 2013 (2013), Article ID 757451, 5 pages

http://dx.doi.org/10.5402/2013/757451

Research Article

Cross-Sectional Study on the Prevalence of Intestinal Parasites and Associated Risk Factors in Teda Health Centre, Northwest Ethiopia

Abraraw Abate, Biniam Kibret, Eylachew Bekalu, Sendeku Abera, Takele Teklu, Aregawi Yalew, Mengistu Endris, Ligabaw Worku, and Zinaye Tekeste

School of Biomedical and Laboratory Sciences, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia

Received 9 May 2013; Accepted 8 July 2013

Academic Editors: R. Kalyanasundaram, K. R. Trenholme, J. Venegas Hermosilla, and R. Zufferey

Copyright © 2013 Abraraw Abate et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract.

Objective. To assess the magnitude of intestinal parasitic infection and associated risk factors in Teda Health Centre, Northwest Ethiopia. Method. A cross-sectional study was conducted in Teda Health Centre from February to April, 2011. Stool samples were collected from 410 study participants and analysed by direct wet mount and formal ether concentration techniques. Furthermore, sociodemographic data were collected by using standardized questionnaire. Result. The overall prevalence of intestinal parasitic infection in this study was 62.3%. Ascaris lumbricoides was the most predominant parasite (23.2%) followed by Giardia intestinalis (12.4%), Entamoeba histolytica/dispar (4.6%), Schistosoma mansoni (8.9%), hookworm (6.6%), Hymenolepis nana (1.5%), Enterobius vermicularis (0.4%), and Strongyloides stercoralis (0.2%). Absence of toilet and hand washing after toilet was shown to be associated with intestinal parasitic infection ( for both). Furthermore, swimming and less shoe wearing habits showed a significant prevalence of S. mansoni and hookworm infections, respectively. Conclusion. The present study showed high prevalence of intestinal parasitic infection in the study area. Absence of toilet and hand washing after toilet was found to be associated with intestinal parasitic infection. Therefore, there is a need for integrated control programme to have a lasting impact on transmission of intestinal parasitic infection.

1. Background

The intestinal parasites can be protozoan or helminth living within the body. Generally, these parasites are more common in tropics and subtropics than elsewhere in the world [1, 2]. It is closely associated with low income, poor personal hygiene, environmental sanitation, lack of pure water supply, limited access to clean water, tropical climate, and low altitude [1, 2].

Entamoeba histolytica/dispar, Ascaris lumbricoides, hookworm, and Trichuris trichiura are among the most common parasites in the world [2]. According to the World Health Organization (WHO) estimates, there are 800–1000 million A. lumbricoides, 700–900 million Hookworm infections, 500 millions T. trichiura, 200 million Giardia intestinalis, and 500 million E. histolytica/dispar cases globally [3]. Despite recent effort to control intestinal parasite infections, the diseases are still the leading causes of mortality and morbidity in the world [2].

In Ethiopia, intestinal parasitic infections are the major causes of mortality and morbidity causing a series of public health problems such as malnutrition, anaemia, and growth retardation as well as higher susceptibility to other infections [4–7]. Poor environmental sanitation, irrigation, overcrowding, resettlement, and low altitude were suggested to be responsible for high prevalence of intestinal parasitic infection in the country [2, 8–12]. However, prevalence of intestinal parasitic infections and the possible risk factors for intestinal parasitic infections were not illustrated in several localities of Ethiopia particularly in the study area. Therefore, the aim of this study was to assess the magnitude of intestinal parasitic infection and related risk factors in Teda Health Center. The research findings could provide data on the distribution and prevalence of intestinal parasites and assist in proposing strategies to protect those groups that might be at risk of intestinal parasitic infection.

2. Materials and Methods

2.1. Study Area and Population

The study was conducted from February to April 2011 in Teda Health Center which is found 24 km from Gondar town, Northwest Ethiopia. Teda town has an average altitude of 1800–2600 meters above sea levels, and the mean rainfall is 771–1160 mm. The town has two primary schools, one high school, one college, and one health centre.

A total of 410 individuals participated in the study. Individuals, who had no history of anti-intestinal drug/s in the two weeks prior to screening, absence of any other serious chronic infection and those who had an ability to give stool samples were included in the study.

2.2. Data Collection and Processing

Sociodemographic data were collected using standardized questioners. Stool specimens were collected using clean plastic sheet from all the study participants at Teda Health Center. Before starting the actual work, the specimens were checked for serial number, quantity, and procedures of collection. Furthermore, quality of reagents and instruments was checked by experienced laboratory technicians. Both saline wet mount and formal ether concentration techniques were used for examination of intestinal parasites as described by the WHO [13].

2.3. Data Analysis

The collected data were checked for completeness and analysed using Statistical Package for Social Sciences (SPSS) (16.0 version) and Simple Interactive Statistical Analysis (SISA) software. Chi square was used to determine association. Values were considered to be statistically significant when values were less than 0.05.

2.4. Ethical Considerations

The study protocol was reviewed and approved by the Ethical Review Committee of Department of Medical Laboratory Sciences, University of Gondar. Written informed consent was obtained from all study participants and mothers/caretakers of children under 18 years old who participated in the study after explaining the purpose and objective of the study.

3. Results

3.1. General Characteristics of the Study Participants

A total of 410 subjects participated in this study, among them 198 (48.3%) were males and 212 (51.7%) were females. One hundred fifty one (36.8%) study participants were age group ≤14 years (Table 1). About 184 (44.9%), 143 (34.9%), 71 (17.3%), and 12 (2.9%) of the study participants were illiterate, elementary school students, high school students, and college or above, respectively (Table 1). Furthermore, 112 (27.5%), 129 (31.5%), 82 (20%), and 87 (21%) of the study participants get water from well, river, spring, and pipe water, respectively (Table 1).

Table 1: The prevalence of intestinal parasitic infection with respect to sociodemographic characteristics of the study participants at Teda Health Centre from February to April, 2011.

Characters
Positive
Negative
Total
values

Number
%
Number
%
Number
%

Sex

    Male
123
62.1
75
37.9
198
48.3
0.97
0.89

    Female
132
62.3
80
37.7
212
51.7

Age

    14
89
58.9
62
41.1
151
36.8
0.639
1.69

    15–29
87
66.4
44
33.6
131
31.9

    30–44
41
62.1
25
37.9
66
16.1

    >45
38
61.3
24
38.7
62
15.2

Education status

    Illiterate
121
65.8
63
34.2
184
44.9
0.28

3.770

    Elementary school
88
61.5
55
38.5
143
34.9

    High school level
41
57.7
30
42.3
71
17.3

    College and above
5
41.7
7
58.3
12
2.9

Hand washing habit

    Before meal

        Yes
138
64.2
77
35.8
215
52.4
0.383
0.076

        No
117
60
78
40
195
47.6

    After toilet

        Yes
144
80.9
34
19.1
178
43.4
<0.01≠
46.8

        No
111
47.8
121
52.2
232
56.6

Source of water supply

    Pipe
53
60.9
34
39.1
87
21
0.087
0.69

    River
78
60.5
51
39.5
129
31.5

    Spring
54
65.9
28
34,1
82
20

    Well
70
62.5
42
37.5
112
27.5

Toilet

    Present
106
56.4
82
43.6
188
45.9
0.02≠
4.99

    Absent
149
67.2
73
32.9
222
54.1

Shoe wearing habit*

    Yes
7
4.2
161
95.8
168
41
0.04≠
4.17

    No
23
9.5
219
90.5
242
59

Swimming habit**

    Yes
42
18.9
180
81.1
222
54.1
<0.001≠
17.0

    No
10
5.3
178
94.7
188
45.9

Overall
255
62.2
155
37.8
410
100

Only for Hook worm, only for Schistosoma mansoni, significant difference.

3.2. Prevalence of Intestinal Parasitic Infection

The overall prevalence of intestinal parasitic infection in Teda Health Center was 62.2% (255/410). Out of the 198 (48.3%) male and 212 (51.7%) female study participants, 62.1% (123/198) and 62.3% (132/212) were positive for at least one intestinal parasite, respectively. However, there was no significant association between sex and intestinal parasitic infection () (Table 1).

About 65.8%, 61.1%, 57.7%, and 41.7% of the study participants categorized under illiterate, elementary school, high school, and college educational categories were positive for intestinal parasitic infection, respectively. However, there was no significant association between intestinal parasitic infection and education status () (Table 1).

Of the total study participants, about 4.6%, 12.4%, and 1.5% of the study participants were infected with E. histolytica/dispar, G. intestinalis, and Hymenolepis nana, respectively (Figure 1). Ascaris lumbricoides (23.2%) was the predominant parasite followed by G. intestinalis (12.4%) and Schistosoma mansoni (8.9%) (Figure 1). Furthermore, there were 2.20%, 1.70%, 1.95%, and 1.22% A. lumbricoides and G. intestinalis, A. lumbricoides and S. mansoni, S. mansoni and G. intestinalis, and hookworm and E. histolytica/dispar cases, respectively (Figure 2).

Figure 1: Prevalence of single parasitic infection in the study participants at Teda Health Centre from February to April, 2011.

Figure 2: Prevalence of multiple parasitic infections in the study participants at Teda Health Centre from February to April, 2011.

3.3. Associated Risk Factors

There was a higher percentage of intestinal parasitic infection in individuals who wash their hand before meal (64.2%) than those who do not (60%); the difference was not statistically significant () (Table 1). Furthermore, there was a significant association between hand washing after toilet with intestinal parasitic infection (). Study participants who had swimming habit in rivers and less shoe wearing habit showed a statistically significant association with high prevalence of S. mansoni and Hook work, respectively ( for all) (Table 1).

4. Discussion

The demonstration that there was high prevalence of intestinal parasitic infection in this study was inconsistent with studies conducted in other regions of the country [14, 15]. For instance, a study conducted among school children in Jimma [15] town showed lower prevalence (47.1%) of intestinal parasitic infection than in the present study. Furthermore, lower prevalence (34.2%)of intestinal parasitic infection was reported from a study conducted in Northwest Ethiopia [14]. In contrast, higher prevalence of intestinal parasitic infection was reported from Azezo (72.9%) [16], Jimma (83%) [15], and Southeast of Lake Langano (83.8%) [17]. The contradictory report on the prevalence of intestinal parasitic infection could be due to variation in awareness regarding transmission and prevention of intestinal parasites between study participants in this study and previous studies.

In Ethiopia, prevalence of ascariasis was reported to be 37%, which is higher than the prevalence of ascariasis in the present study [18]. Furthermore, studies conducted in Gondar and Southeast of Lake Langano also reported 5.9% and 6.2% prevalence of Ascaris lumbricoides, respectively [14, 17]. Furthermore, the demonstration that there was 7.30%, 0.50%, 10.50%, 12.90%, and 17.30% prevalence of Hook worm species, S. stercoralis, S. mansoni, E. histolytica/dispar, G. intestinalis, and H. nana was not in agreement with previous studies conducted in Ethiopia [14, 15]. For instance, as compared to the present study, lower prevalence of Hook worm and Schistosoma mansoni were reported among school children in Gondar [14]. In contrast, Mengistu [15] reported high prevalence of Hook worm and S. mansoni in urban communities of South-western Ethiopia.

The present study did not find a significant difference between intestinal parasitic infections and hand washing practice before meal (). This was inconsistent with studies conducted in Gondar and Babile [14, 19], where there was significant association between intestinal parasitic infections and hand washing practice. This difference might be due to the usage of contaminated water by the study participants for different purposes.

Although slightly higher prevalence rate was reported among illiterates, there was no significant association between education status and intestinal parasitic infections (Table 1). Similar finding was reported from a study conducted in Zarima town [20]. Higher level of education is usually associated with higher level of hygiene which might reduce the prevalence of parasitoses. This was not shown in our study; this might be due to the few number of study participants with college and above education (2.9%). Furthermore, a significant difference was observed in hand washing practice after toilet and absence of toilet with intestinal parasitic infection. The contrary association between hand washing practice after toilet and intestinal parasitic infection might be due the habit of using only water for washing in the area and inappropriate handling of readymade foods and drinks, without washing their hands using soap/ash.

Swimming and less shoes wearing habits showed a statistically significant association with prevalent S. mansoni and Hook worm infections, respectively (Table 1). These results were consistent with the reports by Endris et al. at Azezo [16]. The prevalence of S. mansoni was higher in study participants who had swimming habit (18.9%) than who did not. This might indicate the presence of infested water body/ies in the study area. However, age, sex and source of water did not show statistically significant associations with intestinal parasitic infections.

In conclusion, the present study showed high prevalence of intestinal parasites in the study area. Furthermore, absence of toilet and hand washing after toilet was found to be associated with intestinal parasitic infection. Swimming and less shoes wearing habits showed a statistically significant association with prevalent S. mansoni and Hook worm infections, respectively. Therefore, there is a need for integrated control program including periodic deworming, construction of public toilets, creating awareness regarding the importance of washing hands after toilet and the impact of swimming in contaminated water to have a lasting impact on transmission.

Conflict Interests

The authors declare no conflict of interest.

Acknowledgments

The authors like to acknowledge Teda Health Centre laboratory administrators for their cooperation during data collection.

References

F. Carneiro, E. Cifuentes, and M. Tellez-Rojo, “Environmental health indicators and development of preventive actions against Ascaris lumbricoides infections in rural communities, Caparao and Alto Caparao, Minas Gerais, Brazil,” Epidemiology, vol. 11, no. 4, p. S63, 2000.

S. Tedla, “Intestinal helminthiasis in Ethiopia,” Helminthologia, vol. 23, pp. 43–48, 1981.

Prevention and Control of Intestinal Parasitic Infections, WHO Technical Report Series 741, WHO, Geneva, Switzerland, 1987.

N. R. de Silva, H. L. Guyatt, and D. A. Bundy, “Morbidity and mortality due to ascaris-induced intestinal obstruction,” Transactions of the Royal Society of Tropical Medicine and Hygiene, vol. 91, pp. 31–36, 1997.

L. S. Stephenson, M. C. Latham, E. J. Adams, S. N. Kinoti, and A. Pertet, “Physical fitness, growth and appetite of Kenyan school boys with hookworm, Trichuris trichiura and Ascaris lumbricoides infections are improved four months after a single dose of albendazole,” Journal of Nutrition, vol. 123, no. 6, pp. 1036–1046, 1993.

C. Nokes and D. A. Bundy, “Compliance and absenteeism in school children: implications for helminth control,” Transactions of the Royal Society of Tropical Medicine and Hygiene, vol. 87, pp. 148–152, 1993.

P. Hadidjaja, E. Bonang, M. A. Suyardi, S. A. N. Abidin, I. S. Ismid, and S. S. Margono, “The effect of intervention methods on nutritional status and cognitive function of primary school children infected with Ascaris lumbricoides,” American Journal of Tropical Medicine and Hygiene, vol. 59, no. 5, pp. 791–795, 1998.

S. Tedla and L. Jemaneh, “Distribution of Ancylostoma duodenale and Necatoramericanus in Ethiopia,” Ethiopian Medical Journal, vol. 23, pp. 149–158, 1985.

A. Z. Zein and M. Assefa, “The prevalence of intestinal parasites among farming cooperatives gondar region northwestern ethiopia,” Ethiopian Medical Journal, vol. 23, pp. 159–167, 1985.

E. McConnell and J. C. Armstrong, “Intestinal parasitism in fifty communities on the central plateau of Ethiopia,” Ethiopian Medical Journal, vol. 14, pp. 159–170, 1976.

S. Tedla and T. Ayele, “Ascariasis distribution in Ethiopia,” Ethiopian Medical Journal, vol. 24, pp. 79–86, 1986.

P. O’Lorrcain and C. V. Holland, “The public health importance of Ascaris lumbricoides,” Parasitology, vol. 121, pp. S51–S71, 2000.

WHO, Basic Laboratory Methods in Medical Parasitology, World Health Organization, Geneva, Switzerland, 1991.

A. Gelaw, B. Anagaw, B. Nigussie et al., “Prevalence of intestinal parasitic infections and risk factors among schoolchildren at the University of Gondar Community School, Northwest Ethiopia: a cross-sectional study,” BMC Public Health, vol. 13, article 304, 2013.

A. Mengistu, “Prevalence of intestinal parasitic infections among urban dwellers in southwest Ethiopia,” The Ethiopian Journal of Health Development, vol. 21, no. 1, pp. 12–17, 2007.

M. Endris, W. Lemma, Y. Belyhun, B. Moges, A. Gelaw, and B. Angaw, “Prevalence of intestinal parasites and associated risk factors among students of Atse Fasil general elementary school Azezo, Northwestern Ethiopia,” Ethiopian Journal of Health and Biomedical Sciences, vol. 3, no. 1, pp. 25–33, 2010.

M. Legesse and B. Erko, “Prevalence of intestinal parasites among school children in rural area close to the south east of Lake Langano, Ethiopia,” The Ethiopian Journal of Health Development, vol. 18, no. 2, pp. 116–120, 2004.

Z. Tadesse, A. Hailemariam, and J. H. Kolaczinski, “Potential for integrated control of neglected tropical diseases in Ethiopia,” Transactions of the Royal Society of Tropical Medicine and Hygiene, no. 102, pp. 213–214, 2008.

G. Tadesse, “The prevalence of intestinal helminthic infections and associated risk factors among schoolchildren in Babile town Eastern Ethiopia,” The Ethiopian Journal of Health Development, vol. 19, no. 2, pp. 140–147, 2005.

A. Alemu, A. Atnafu, Z. Addis et al., “Soil transmitted helminths and Schistosoma mansoni infections among school children in Zarima town, northwest Ethiopia,” BMC Infectious Diseases, vol. 11, article 189, 2011.