Phytochemical Profiling of Leaf, Stem, and Tuber Parts of Solena amplexicaulis (Lam.) Gandhi Using GC-MS

Objective. To explore the possible bioactive compounds in the methanolic extracts of leaf, stem, and tuber parts of the medicinal climber, Solena amplexicaulis, using GC-MS. Methods. GC-MS analysis of the plant extracts were performed by using GC-MS-5975C [Agilent] and mass spectra of the compounds found in the extract was matched with the data in the library of National Institute of Standards and Technology (NIST). Results. Thirty-five compounds were determined to be present in the parts studied. The active principles with their retention time, molecular formula, molecular weight, peak area, structure, category of the compounds, and activities were predicted. The most prevailing compounds were phytol (38.24%) in leaf, 4-(4-ethoxyphenyl) but-3-en-2-one (56.90%) in stem, and 9,17-octadecadienal, (Z)- (21.77%) in tuber. Conclusion. This study revealed that the species S. amplexicaulis is a good source of many bioactive compounds like terpenes, triazines, esters, alkanes, alcohols, hydrocarbons, aldehydes, amides, and so forth. That justifies the traditional usage of this species.


Introduction
Herbal plants are valuable gift of nature for mankind and they are the source of a variety of phytochemicals which are utilized for human and animal diets also. It is capable of synthesizing an overwhelming variety of low molecular weight organic compounds called secondary metabolites, usually with unique and complex structures. The medicinal actions of plants unique to particular plant species or groups are consistent with the concept that the combination of secondary products in a particular plant is taxonomically distinct [1]. It states that around 85-90% of the world's population consumes traditional herbal medicines [2]. In recent decades, studies on phytochemical constituents of medicinal plant and its pharmacological activities have received wide attention [3][4][5][6]. WHO has emphasized the need to ensure the quality of medicinal plant products using modern techniques with the application of suitable standards. Many modern methods are adapted for identification and quantification of active principle compounds in plant materials. Of them, gas chromatography-mass spectrometry (GC-MS) has become firmly established as a key technological platform for secondary metabolite profiling in both plant and nonplant species [7,8].
The plant species Solena amplexicaulis is commonly called creeping cucumber and belongs to the family Cucurbitaceae distributed very seldom in the dry deciduous forest and scrub jungles of Tamil Nadu [9]. The medicinal uses of this species are multifaceted. The local healers of Tamil Nadu and Andhra Pradesh are prescribing this species for many ailments owing to its effective healing property [10]. The traditional healers are prescribing the tubers, leaves, and seeds of this species for various ailments like spermatorrhoea, thermogenics, diuretics, haemorrhoids, and invigorating and it is a very good appetizer and cardiotonic [11]. The whole plant is a potential source of natural antioxidant [12,13], antidiabetic [10], and antibacterial agent [14] also. As the leaves have good anti-inflammatory activity, it is recommended for inflammation, skin lesions, and skin diseases [15]. Crude leaf juice is used to cure jaundice [16]. Unripe fruits are eaten raw to strengthen the body [17]. The decoction of the root is taken orally to cure stomachache [18]. As the reproductive parts like 2 International Scholarly Research Notices seeds and tubers are exploited severely for medicinal uses, this species becomes rare sighted in its habitats of Tamil Nadu.
Despite these wide medicinal uses, no information on qualitative account of phytochemicals is available for this species. To address this lacuna, GC-MS studies were undertaken to explore the phytochemical constituents present in the leaf, stem, and tuber parts of S. amplexicaulis.

Collection, Identification and Preparation of Plant
Materials. The leaf, stem, and tuber parts of S. amplexicaulis were collected separately from the thorny scrub jungles of Madukkarai, Coimbatore District, Tamil Nadu, India. The authenticity of the plant was confirmed in Botanical Survey of India, Southern Regional Centre, Coimbatore, by referring to the deposited specimen (Voucher specimen number: CPS 313). They were washed thoroughly in tap water, shade-dried, and then homogenized to fine powder and stored in air tight bottles.

Preparation of Extract
. 50 g of powdered leaf, stem, and tuber parts of S. amplexicaulis was separately extracted with 250 mL methanol at the temperature between 60 and 65 ∘ C for 24 h by using soxhlet extractor. The solvent was evaporated by rotary vacuum evaporator to obtain viscous semisolid masses. This semidry methanolic crude extract was subjected to GC-MS analysis.

GC-MS Analysis.
GC-MS analysis was carried out on a 5975C Agilent equipped with a DB-5ms Agilent fused silica capillary column (30 × 0.25 mm ID; film thickness: 0.25 m), operating in electron impact mode at 70 eV. Pure helium (99.999%) was used as carrier gas at a constant flow of 1 mL/min and an injection volume of 1 L was employed (split ratio is 10 : 1). Mass transfer line and injector temperature were set at 230 and 250 ∘ C, respectively. The oven temperature was programmed from 70 (isothermal for 3 min) to 300 ∘ C (isothermal for 9 min) at the rate of 10 ∘ C/min. Total GC running time was 34 min and the MS detection was completed within 35 min.
By GC-MS, the compounds were separated and then they were eluted from the column and made enter into the detector which was capable of creating an electronic signal. Then they were processed by the computer for generating chromatogram. Then the compound entered into the electron ionization (mass spectroscopy) detector, where they were bombarded with a stream of electrons causing them to break apart into fragments. These fragments were actually charged ions with certain mass. The m/z (mass/charge) ratio obtained was calibrated from the graph, called the mass spectrum, and is the fingerprint of the molecule.

Identification of the Compounds.
To identify the compounds, the extract was assigned for comparison of their retention indices and mass spectra fragmentation patterns with those stored on the computer library and also with the published literature. National Institute of Standards and 5.00 5.00 7.00 7.00 6.00 6.00 10.00 10.00 9.00 9.00 8.00 8  Technology library sources were also used for matching the identified compounds from the plant materials [19,20].

Discussion
The gas chromatogram shows that the relative concentrations of various compounds are getting eluted as a function of retention time. The height of the peaks indicates the relative concentrations of the compounds present in the plant. The mass spectrometer analyzes of the compounds eluted at different times to identify the nature and structure of the compounds. The large compound fragments into small compounds give rise to appearance of peaks at different / ratios. These mass spectra are fingerprint of that compound which can be identified from the data library.
Generally, the reliability of medicinal plant for its usage is evaluated by correlating the phytochemical compounds with their biological activities [21]. In the present study, the GC-MS analysis of the methanolic extracts of leaf, stem, and tuber parts of S. amplexicaulis altogether showed the presence of 35 compounds. In this account, the leaf extract contained six compounds among them, phytol (38.24%) is having anticancer, antioxidant, anti-inflammatory, antitumor, antimicrobial, diuretic, and chemopreventive properties and used in vaccine formulations [22,23]. The other compound, carane (18.76%) is having antifeedant and antioxidant properties [24,25]. The methanolic stem and tuber extracts showed the presence of greater number of 14 and 15 compounds, respectively. The six phytoconstituents, namely, undecane, taurolidine, trehalose, hexadecanoic acid methyl ester, 9octadecenoic acid (Z)-, methyl ester, and benzaldehyde, 2nitro-, diaminomet hylidenhydrazone in stem extracts have possessed medicinal properties [26]. Undecane, an alkane, is an antimicrobial agent, used as carcinogen [27,28]. Similarly, the other compound, taurolidine, a taurine amino acid derivative, has antimicrobial, antilipopolysaccharidal, and antitumor properties [29,30]. The sucrose compound, trehalose, is used for the treatment of amyloidosis [31]. The linoleic acid esters present in the stem, hexadecanoic acid methyl ester, are reported to have anti-inflammatory, cancer preventive, hepatoprotective, antiarthritic, and anticoronary properties. The other linoleic acid ester, 9-octadecenoic acid (Z)-, methyl ester, is also having anti-inflammatory, antiandrogenic, and anemiagenic properties [32]. The nitrogen compound, benzaldehyde, 2-nitro-, diaminomet hylidenhydrazone, is known to have the property of curing infectious diseases by its antimicrobial activity. In the tuber extracts, the compounds identified, namely, 10,13-octadecadienoic acid methyl ester, trans-13-octadecenoic acid, methyl ester, and 9,12-octadecadienoic acid (Z,Z)-, are possessed with anti-inflammatory and cancer preventive characters. The two compounds, namely, tetradecanoic acid and n-hexadecanoic acid, are antioxidants. The phthalic acid, 1,2-benzenedicarboxylic acid, bis(2-methylpropyl) ester, is used in the preparation of perfumes and cosmetics. The unsaturated alcoholic compound, 9,17-octadecadienal, (Z)-, is reported to have antimicrobial property [33]. The study species S. amplexicaulis is endowed with various medicinal properties maybe due to the presence of all these compounds described. In a similar fashion, certain traditional medicinal plant species of Cucurbitaceae have been analyzed phytochemically by using GC-MS and suggested for drug preparation after succeeding in clinical trials [34,35]. The therapeutic properties of the other compounds in all the three parts of S. amplexicaulis were not yet reported.
Our investigation through the present study revealed that the species S. amplexicaulis is a reliable source of bioactive compounds like fatty acid esters, alcohols, hydrocarbons, alkanes, amines, terpenes, and sugars that justify the traditional usage of this species [16][17][18] by the local healers in Coimbatore and Tirupur districts of Tamil Nadu, India, for various ailments. As GC-MS is the first step towards understanding the nature of active principles [36,37], further investigation in this species is suggested for the development of novel drugs.