There is much interest among evolutionary biologists in the genus
The host range for
Here we examine
We acquired 60 southern Louisiana wild caught
Gonad and heart tissue samples were isolated from 60
We used PCR amplification with a multiplex of
Multiplex primers.
Primer | Primer sequence | Approximate fragment size | Source |
---|---|---|---|
|
CATACCTATTCGAAGGGATAG | 450 |
[ |
|
AGCTTCGAGTGAAACCAATTC | ||
wsp_81F | TGGTCCAATAAGTGATGAAGAAAC | 600 |
[ |
wsp_691R | AAAAATTAAACGCTACTCCA | ||
coxA_F1 | TTGGRGCRATYAACTTTATAG | 500 |
[ |
coxA_R1 | CTAAAGACTTTKACRCCAGT | ||
ftsZ_F1 | ATYATGGARCATATAAARGATAG | 550 |
[ |
ftsZ_R1 | TCRAGYAATGGATTRGATAT |
Three positive controls were used, containing DNA from the three lines of
Of the 120 samples tested with the universal invertebrate 16s rDNA primer pair 116 tested positive, yielding fragments approximately 600 base pairs in length and indicating the presence of PCR-viable DNA in the heart and gonad isolates of 57 crayfish. The multiplex used on the isolates from the 57 crayfish failed to amplify any gene fragments.
These results are consistent with a preliminary study of
It is not possible to prove the negative, in this case, that
The probability that at least one individual out of 57 will test positive for
In determining the frequency of
The authors declare that there is no conflict of interests regarding the publication of this paper.
The authors thank Danny Kraemer for crayfish samples and Bethany Cook for crayfish maintenance. This work was supported in part by NSF Grant (DUE 0926743) to W. J. Boecklen and A. C. James.