Advances in Civil Engineering
Volume 2019 (2019), Article ID 4903602, 14 pages
https://doi.org/10.1155/2019/4903602

Research Article

Characterizing Skeleton Structure and Stacking Properties of Continuous and Gap Graded Aggregate Mixtures

Dexiang Bao,1,2 Chen Jiaqi,³ Hao Wang,⁴ Huanjiao Liu,⁵ and Jiupeng Zhang⁵

¹School of Civil Engineering, Lanzhou Jiaotong University, Lanzhou, Gansu 730070, China
²Gansu Province Transportation Planning, Survey & Design Institute Co., Ltd, Lanzhou, Gansu 730070, China
³School of Civil Engineering, Central South University, Changsha, Hunan 410075, China
⁴Department of Civil and Environmental Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA
⁵Key Laboratory for Special Area Highway Engineering of Ministry of Education, Chang’an University, Xi’an, Shaanxi 710064, China

Correspondence should be addressed to Chen Jiaqi; chenjiaqi@csu.edu.cn

Received 20 March 2019; Revised 9 April 2019; Accepted 9 April 2019; Published 12 May 2019

Academic Editor: Ghassan Chehab

Copyright © 2019 Dexiang Bao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract.

In order to optimize the skeletal properties of granular pavement materials, a numerical discrete element model for continuous and gap graded aggregate mixtures was developed using a step-by-step filling method. The penetration tests were conducted, and indexes such as penetrating resistance, average coordination number, California bearing ratio, and void ratio of coarse aggregates obtained from the tests were used to evaluate the skeleton structure and the stacking properties of aggregate mixtures. The results show that the skeleton structure of aggregate mixtures is closely related to the combination of particle size. Smaller difference in particle size leads to stronger interference effect. The parameters of stacking properties of continuous and gap graded aggregate mixtures are not significantly different in the natural stacking condition. However, gap graded aggregate mixtures are affected by external loads significantly due to structural reconstruction.

1. Introduction

The continuous and gap graded aggregates mixtures are commonly used granular materials in the pavement structure. The mechanical properties of these granular materials should be properly evaluated due to their significant effect on the pavement performance. Although the mechanical properties of these pavement materials can be evaluated with traditional experiments from the macroperspective, extensive literatures show that the mesostructure of pavement materials should be further studied since it significantly affects the bulk material properties [1–3].

The discrete element method (DEM) has been widely used to study the mesoscopic properties of pavement materials, since it is an efficient method to simulate the interaction between granular particles [4–7]. Different algorithms have been developed to simulate the geometries of aggregates and ballasts [8–10], and various experiments such as the direct box shear tests, simple shear tests, triaxial tests, and others have been simulated with DEM. Song et al. simulated the mesostructure of the end soil in shield tunnels by using DEM [11]. The mesomechanism for the slipping damage of the end soil was discussed. In order to study the fracture behaviors of asphalt mixture, Chen and Huang conducted virtual center point beam tests with DEM and 2D microstructure of asphalt mixtures [12]. Besides of center point beam tests, virtual biaxial tests based on DEM were also reported in the literatures, to study the effect of particle size, confining pressures, and loading velocities on the elastic moduli, strength, and crack-initiation stress [13, 14]. Lu and Mcdowell investigated the influence of the shape on the heterogeneous stresses within an aggregate and found that more angular shape led to a greater degree of homogeneity [15]. Uthus et al. simulated the resilient response of an unbound granular material subjected to sinusoidal loading in a triaxial sample [16]. Chen et al. conducted virtual process simulation test to investigate the lateral displacement of a granular assembly under cyclic loading [17].

In order to further investigate the skeleton structure and stacking properties of continuous and gap graded aggregate mixtures, virtual penetration tests based on DEM were conducted to study the CBR of different type of aggregate mixtures in this paper. The macro- and mesomechanical correlation of the mineral aggregate mixtures were developed. The optimal filling ratios for these aggregate mixtures were also recommended based on the analysis in this paper.

2. Virtual Experiments and Parameters Calibration

2.1. Development of Digital Specimens and Virtual Test

In this section, digital specimens of aggregate mixtures were developed and used for virtual penetration testing. The simulated aggregates were simplified as spheres with particle size between 4.75 and 16 mm. Aggregate mixtures with different gradations were developed by using aggregates with different sizes. In general, the development process of the digital specimens and virtual testing was divided into three steps.

2.1.1. Generation of Digital Specimens

In this step, the digital specimens with the same dimensions as the specimens used in the CBR test were developed. Firstly, a virtual cuboid space with 150 mm in length, 150 mm in width, and 170 mm in height was set up for the placement of aggregates. Then, particles with different sizes were placed in the above virtual cuboid space randomly, considering the prescribed gradations. The placement of the particles was achieved by using specific flow code in PFC3D. In this process, due to the initial overlap between particles, unbalanced internal force would be caused between particles. In order to eliminate the overlap between particles, multiple cycles were applied to the specimen until the unbalanced internal force reached the minimum value. After this process, a cuboid specimen was developed as shown in Figure 1(a). In order to get the cylindrical specimen with the same dimensions as that used in the CBR test, an internal cylinder with 150 mm in diameter and 170 mm in height was cut from the above cuboid specimen. The particles inside the cylinder were kept, while the others were deleted, so that a cylindrical specimen of aggregate mixture was developed as shown in Figure 1(b).

(a)

(b)

(c)

Figure 1: Digital simulation specimen.

2.1.2. Compaction of Digital Specimens

The CBR value of aggregate mixture was affected by the compaction state. Therefore, the loose digital specimens generated in Step (1) should be compacted before conducting virtual penetration test, in order to ensure adequate contact between particles. In this step, a vertical displacement with velocity of 1 mm/s was applied to the top surface of the cylindrical specimen. During this process, the average contacts between particles within the blue sphere shown in Figure 1(c) were monitored and analyzed. When the change of the average contacts between particles became relative stable, the compaction of the digital specimen was completed.

2.1.3. Virtual Penetration Test

A penetration rod with 50 mm in diameter and 100 mm in height was generated, as shown in Figure 2. This rod was penetrated into the aggregate mixture with a constant velocity of 50 mm/min, until the penetration depth reached 20 mm.

Figure 2: Penetration process.

During this process, the displacement, stress, and pressure at the bottom of the rod were recorded and were used to calculate the CBR value according to equation (1), after the virtual testing was finished. The CBR value was calculated when the displacement was 2.5 mm.

2.2. Calibration of Mesoscopic Parameters

The mesoscopic parameters used in PFC3D should be calibrated before conducting numerical analysis, due to the lack of direct relationship between the macromechanical properties and the mesoscopic parameters [18]. In this paper, the single-variable method was used to calibrate the mesoscopic parameters, including the friction coefficient, porosity, and contact stiffness ratio between particles, due to their important effect on the macroscopic properties of the bulk granular material reported in the literatures [19, 20].

The friction coefficient reflects the roughness of the contact surface between aggregates and usually ranges between 0 and 1. In this section, the friction coefficient was taken as five different values, namely, 0.1, 0.2, 0.3, 0.4, and 0.5. Virtual penetration test was conducted with these friction coefficient values, and the result was shown in Figure 3. Besides, seven different porosities (0.2, 0.25, 0.3, 0.35, 0.4, 0.45, and 0.5) and five different contact stiffness ratios (1, 2, 3, 4, and 5) were selected to analyze the unit pressure with penetration of 5 mm. The results are shown in Figures 4 and 5, respectively.

Figure 3: Unit pressure and penetration under different friction coefficients.

Figure 4: Variation in porosity.

Figure 5: Variation in contact stiffness ratio.

It can be seen from Figure 3, the simulation results with the friction coefficient of 0.4 matched with the measured values best. Therefore, the friction coefficient was taken as 0.4 in the following studies. Figures 3–5 also indicate that the penetration force was more sensitive to the friction coefficient and the porosity than the contact stiffness ratio. The porosity and the contact stiffness ratio were taken as 0.35 and 1, based on Figures 4 and 5, respectively.

2.3. Evaluation Indexes of Skeleton Structure and Stacking Properties

The CBR value is a commonly used index to evaluate the quality of the coarse aggregate skeleton structure [21, 22]. The studies of stacking problem can be traced back to the famous Kepler conjecture and the 13-ball debate between Newton and Gregory. In 1968, Mason measured the position coordinates of randomly arranged smooth steel balls by the mosaic technique, and studied the geometrical indexes such as arrangement density, coordination number distribution frequency, and radial distribution function of this array [23]. Richard et al. [24] used X-ray Computed Tomography (CT) to study the variation of void ratios in the process of compacting of beads packing under vertical vibrations. In 2008, An et al. [25] analyzed the effect of amplitude, frequency, and friction coefficient on the coordination number, radial distribution equation, and void structure of the filling process using discrete element method. Mostofinejad and Reisi [26] simulated the filling of coarse aggregates, which were regarded as spheres with different sizes, and proposed a method for predicting the internal void ratios of the aggregate mixtures. In this paper, indexes such as penetrating resistance, average coordination number, California bearing ratio, and void ratio of coarse aggregates were investigated.

3. Characterizing Skeleton Structure of Mineral Aggregates Mixtures

The effects of different aggregate combinations on the skeleton structure of mineral aggregate mixtures were studied based on the analysis of CBR, void ratio of coarse aggregates (VCA), and penetration resistance. A step-by-step filling method was used in the analysis. Studies show that aggregates with the particle size of 7.1 mm have significant influence on the void ratios of the mixture. Therefore, the progressive filling tests in this paper considered four different aggregate sizes and their combinations. The four different sizes of the aggregates were 13.2–16 mm (S1), 9.5–13.2 mm (S2), 7.1–9.5 mm (S3), and 4.75–7.1 mm (S4).

3.1. Skeleton Structure of Continuous Graded Aggregates

Based on the method of progressive filling and the principle of continuous gradation, the first-stage virtual filling tests were conducted. Three kinds of aggregate mixtures with two particle sizes and continuous gradation were prepared and were marked as S12, S23, and S34, respectively. S12 represented for the mixtures in which aggregates S1 were filled by the aggregates S2 with different proportions. Similarly, S23 represented for the mixtures in which aggregates S2 were filled by the aggregates S3 with different proportions. And S34 represented for the mixtures in which aggregates S3 were filled by the aggregates S4 with different proportions. The values of CBR and VCA of these three kinds of aggregate mixtures were obtained by PFC3D and are shown in Figure 6.

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(b)

(c)

Figure 6: CBR and VCA of three kinds of two-aggregate mixtures with continuous gradation (a) S12, (b) S23, and (c) S34.

It can be seen that the variations of CBR and VCA for the three mixtures presented negatively correlated trends. The CBR value increased when the VCA value decreased and the CBR value decreased when the VCA value increased. According to the filling interference principle, the pores in the skeleton structure of the coarse aggregates were filled with the secondary aggregates. However, the skeleton structure formed by the coarse aggregates would be interfered by the excessive secondary aggregates, resulting in the skeleton structure being broken and the CBR value decreasing. Therefore, the optimal filling ratio of each aggregate mixture was be obtained, namely, S1 : S2 = 57 : 43, S2 : S3 = 82 : 18, and S3 : S4 = 90 : 10, based on the principle that smaller VCA led to larger CBR of the skeleton capacity.

The secondary filling was performed based on the above three optimal combinations. Two kinds of aggregate mixtures with three particle sizes and continuous gradation were prepared and were marked as S123 and S234, respectively. S123 represented the mixtures in which S12 was filled by S3 with different proportions, and S234 represented the mixtures in which S23 was filled by S4 with different proportions. The values of CBR and VCA of the above two mixtures are shown in Figure 7.

(a)

(b)

Figure 7: CBR and VCA of two kinds of three-aggregate mixtures with continuous gradation (a) S123 and (b) S234.

It can be seen from Figure 7(a) that the maximum CBR value was obtained when the mass content of S3 was 30%. The skeleton compactness and skeleton strength of the combination were both good when the CBR value reached the maximum value and the VCA reached the minimum value. Therefore, the ratio of S12 to S3 was determined to be 70 : 30, which meant the optimal ratio between S1, S2, and S3 was 39.9 : 30.1 : 30. It can be seen from Figure 7(b) that the CBR value reached the maximum value when the mass content of S4 was 60%. Therefore, the ratio of S23 to S4 was determined to be 40 : 60 and the optimal ratio between S2, S3, and S4 was 32.8 : 7.2 : 60.

The variation of the penetration pressure with the penetration displacement for different mixtures could be obtained during the penetration process. The penetration loads for different aggregate mixtures at the same penetration displacement of 6 mm were recorded. Different penetration loads for the three-aggregate mixtures with two particle sizes (S12, S23, and S34) and the two-aggregate mixtures with three particle sizes (S123 and S234) at the penetration displacement of 6 mm are shown in Figure 8.

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(b)

Figure 8: Penetration loads of five kinds of mixtures at the penetration displacement of 6 mm (a) two-aggregate mixtures and (b) three-aggregate mixtures.

According to Figure 8, the variation trend of the penetration load for different mixtures was in accordance with the variation of the CBR value when the penetration displacement was 6 mm, which increased at first and then decreased gradually. As for the penetration loads, the conclusion of S12 > S23 > S34 and S123 > S234 could be obtained, which proved that the aggregates with larger particle sizes were the main part of skeleton structure. The compressive load was mainly transmitted through the stronger skeleton. Therefore, the mechanical characteristics for the skeleton structure of the aggregate mixtures could be expressed by both absolute differences between CBR and VCA curves and the variation curve of the penetration load at the same penetration depth.

3.2. Skeleton Structure of Gap Graded Aggregates Mixtures

Three kinds of aggregate mixtures with two particle sizes and gap gradation were prepared and were marked as S13, S14, and S24, respectively. S13 represents mixtures in which aggregates S1 were filled by the aggregates S3 with different proportions. Similarly, S14 represented the mixtures in which aggregates S1 were filled by the aggregates S4 with different proportions, and S24 represented the mixtures in which aggregates S2 were filled by the aggregates S4 with different proportions. The values of CBR and VCA of these three mixtures are shown in Figure 9.

(a)

(b)

(c)

Figure 9: CBR and VCA of three kinds of two-aggregate mixtures with gap gradation (a) S13, (b) S24, and (c) S14.

It can be seen from Figure 9 that the optimal ratio could be determined as S1 : S3 = 75 : 25, S2 : S4 = 90 : 10, and S1 : S4 = 40 : 60, based on the principle that smaller VCA led to greater skeleton load capacity characterized by CBR.

Two kinds of aggregate mixtures with three particle sizes and gap gradation were prepared and were marked as S124 and S134, respectively. S124 represented the mixtures in which S12 was filled by S4 with different proportions, and S134 represented the mixtures in which S13 was filled by S4 with different proportions. The values of CBR and VCA of these two mixtures are shown in Figure 10.

(a)

(b)

Figure 10: CBR and VCA of two kinds of three-aggregate mixtures with gap gradation (a) S124 and (b) S134.

It can be seen from Figure 10 that the CBR values of these two mixtures increased firstly and then decreased later. The optimal ratio of S1 and S2 was 57 : 43, and the optimal ratio of S1 and S3 was 75 : 25. The optimal ratios of these gap graded aggregate mixtures with three particle sizes were determined as S1 : S2 : S4 = 22.8 : 17.2 : 60, and S1 : S3 : S4 = 54 : 8 : 18.

Different penetration loads for these gap graded aggregate mixtures with two or three particle sizes at the penetration displacement of 6 mm are shown in Figures 11 and 12.

Figure 11: Penetration loads of three kinds of two-aggregate mixtures with gap gradation at the penetration displacement of 6 mm.

Figure 12: Penetration loads of two kinds of three-aggregate mixtures with gap gradation at the penetration displacement of 6 mm.

It can be seen from Figures 11 and 12 that the mean penetration loads for the gap graded aggregate mixtures with three particle sizes were larger than that with two particle sizes, which proved that, for the load resistance, the gap graded aggregate mixtures with three particle sizes were better than that with two particle sizes. According to Figure 11, the contribution rate on load resistance of S13 was greater than that of S14 and S24 for the gap graded aggregate mixtures with two particle sizes. It can be speculated that the contribution rate on load resistance of different aggregates rely on their particle size, and larger particle size leads to greater contribution rate.

3.3. Comparative Analysis for Continuous and Gap Graded Aggregates Mixtures

The CBR values of different secondary aggregate mixtures are shown in Figure 13. Figure 13 shows that the CBR values firstly increased and then decreased with the variation of the secondary aggregate contents for the continuous gradation. While for the gap gradation, this trend was not so obvious, especially for the mixture S134. The maximum value of CBR was about 125% for the continuous gradation aggregate mixtures and about 100% for the gap gradation aggregate mixtures. The CBR value for the gap gradation aggregate mixtures was smaller than that of the continuous gradation aggregate mixtures under different contents of the secondary aggregates.

Figure 13: Influences on the CBR of different secondary aggregates mixtures.

4. Characterizing Stacking Properties of Mineral Aggregates Mixtures

4.1. Stacking Properties of Continuous Graded Aggregates Mixtures

The influence of the content of secondary aggregates on the VCA value is shown in Figure 14.

Figure 14: Influence of the content of secondary aggregate on the VCA.

It can be seen from Figure 14 that with the increase of the content of the secondary aggregates, the void ratios of these mixtures firstly decreased and then increased. The VCA reached the minimum value when the content of the secondary aggregates was 30%.

The variation of the initial average coordination number with the content of the secondary aggregates is shown in Figure 15.

Figure 15: Variation of the initial average coordination number with the content of secondary aggregates.

It can be seen from Figure 15 that the variation of the initial average coordination number were less than 5%, meaning that variation range of the initial average coordination number of the continuous graded mixtures was very small and could be treated as a constant.

According to the number of contact points and the number of particles collected during the penetration test, the average coordination numbers of the continuous graded mixtures were calculated by the weighted mean method and are shown in Figure 16, where S represented the penetration displacement. It can be seen from Figure 16 that the average coordination numbers of the continuous graded mixtures increased with the increase of the displacement. Therefore, the conclusion is drawn that the good filling skeleton structure is formed for the continuous gradation mixtures, and the structure properties can ensure a good condition under the load.

(a)

(b)

(c)

Figure 16: Variation of the average coordination numbers with different penetration displacements (a) S = 6 mm, (b) S = 12 mm, and (c) S = 20 mm.

4.2. Stacking Properties of Gap Graded Aggregates Mixtures

The VCA of the gap graded aggregate mixtures with three particle sizes is shown in Figure 17.

Figure 17: Influence of the content of secondary aggregates on the VCA.

It can be seen from Figure 17 that with the increasing content of the secondary aggregates, the void ratios of these mixtures firstly increased and then decreased. The minimum value of the VCA was obtained when the content of the secondary aggregates was 62%. There are many studies on the distribution of the coordination numbers for single sized aggregates, but little study has been conducted on the distribution of the coordination numbers for the multisized aggregates. The paper reveals that the correlation between the average coordination number and the VCA of mixtures with multisized particles is similar with the mixtures consisting of single-sized aggregates, namely, the average coordination number increases with the decrease of the VCA [27].

The variation of the initial average coordination number of the gap graded mixtures with the content of the secondary aggregates is shown in Figure 18. It can be seen from Figure 18 that the variation of the initial average coordination number of the gap graded mixtures is not significant (less than 5%).

Figure 18: Variation of the average coordination number with the content of secondary aggregates.

The average contact numbers of the continuous graded mixtures under different penetration displacements were calculated. The results are shown in Figure 19.

(a)

(b)

(c)

Figure 19: Variation of the average coordination numbers with different penetration displacements (a) S = 6 mm, (b) S = 12 mm, and (c) S = 20 mm.

As for the mixtures with gap gradation, it can be seen from Figure 19 that the average coordination number increased all the time when S = 6 mm. While when S = 12 mm, the average coordination number firstly increased and then decreased. When S = 20 mm, the average coordination number firstly increased and then decreased, but finally increased again.

4.3. Comparative Analysis for Continuous and Gap Graded Aggregates Mixtures

The influence of the content of the secondary aggregates on the VCA is shown in Figure 20.

Figure 20: Influence of the content of secondary aggregates on the VCA.

It can be seen from Figure 20 that the VCA values firstly decreased and then increased with the variation of the secondary aggregate contents both for the continuous and gap gradation aggregate mixtures. The minimum value of VCA was about 30% for the continuous gradation aggregate mixtures and 60% for the gap gradation aggregate mixtures. The VCA value for the gap gradation aggregate mixtures was larger than that of the continuous gradation aggregate mixtures under different contents of the secondary aggregates.

The variation of the average coordination number with volume fractions of smaller sized aggregates for the continuous and gap gradation mixtures is shown in Figure 21.

Figure 21: Variation of the average coordination number with the content of the secondary aggregates.

It can be seen from Figure 21 that the variation of the average coordination number for the gap gradation mixtures was larger than that of the continuous gradation mixtures, meaning that the staking characteristics of gap graded mixtures was easy to be influenced by the content of the secondary aggregates.

The average contact numbers of the mixtures with continuous and gap gradation in the case of different penetration displacements were calculated. The results are shown in Figure 22.

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(b)

(c)

Figure 22: Variation of the average coordination numbers with different penetration displacements: (a) S = 6 mm, (b) S = 12 mm, and (c) S = 20 mm.

It can be seen from Figure 22 that the average coordination numbers of the mixtures depended on the penetration displacement. For the continuous gradation mixtures, the average coordination number would increase evenly with the increase of the penetration displacement. As for the gap gradation mixtures, the average coordination number increased all the time when S = 6 mm, while increased firstly and then decreased when S = 12 mm. When S = 20 mm, the average coordination number firstly increased and then decreased, but finally increased again. It shows that the external load disturbed the gap gradation mixtures significantly, and the reorganization occurred in the particle mixture structure during the penetration process.

5. Conclusions

In this paper, DEM was adopted to simulate the stacking behavior and the bearing capacity of continuous and gap graded aggregate mixtures. The evolution rule of aggregate particles under different penetration displacements was quantitatively analyzed. The main conclusions are shown as follows:

(1)The values of CBR and VCA for the continuous and gap graded aggregate mixtures were obtained under different contents of the secondary aggregates based on virtual penetration tests. The simulation results indicated that the relationship between CBR and VCA was negatively correlated. The optimal filling ratio of these aggregates mixtures was proposed.(2)The skeleton structure of mineral aggregates mixtures was analyzed. The CBR value increased firstly and then decreased with the increasing content of the secondary aggregates for the continuous and gap graded aggregates mixtures. However, the variation of CBR values for the continuous graded aggregates mixtures was larger than that for the gap gradation aggregates mixtures, meaning that the smaller the difference of particle size is, the stronger the interference effect is.(3)Comparative analysis of stacking properties of mineral aggregate mixtures was conducted to conclude that the parameters of stacking properties of mineral aggregate mixtures with the continuous and gap gradation are not significantly different under natural stacking. But the gap graded aggregates mixtures are affected by external loads obviously resulting in structure reorganization.

Appendix

An Example of Code Used in This Paper

 new title “CBR” domain extent −0.2 0.2 −0.2 0.2 0 0.4 condition destroy cmat default model linear method deformability emod 1e9 kratio 1.0 contact property fric 0.4 range contact type ball-ball cmat default property dp_nratio 0.2 set random 10002 ball distribute porosity 0.36 numbin 2 … bin 1 radius 0.00118 0.002375 volumefraction 0.15 … bin 2 radius 0.002375 0.00475 volumefraction 0.85 … box −0.075 0.075 −0.075 0.075 0 0.17 wall gen box −0.075 0.075 −0.075 0.075 0 0.17 ball attribute density 3000 damp 0.7 cycle 50000 calm 10 save S1_1.p3sav wall delete ball delete range cylinder end1 0 0 0 end2 0 0 0.17 rad 0.075 not wall generate cylinder base 0 0 0 height 0.17 radius 0.075 wall del facets range id 436 496 wall gen id 1000 cylinder base 0 0 0.17 height 0.1 radius 0.025 save S1_2.p3sav cyc 2000 ball attribute displacement multiply 0.0 contact property lin_force 0.0 0.0 0 lin_mode 1 ball attribute contactforce multiply 0.0 contactmoment multiply 0.0 save S1_3.p3sav cyc 2000 ball attribute displacement multiply 0.0 contact property lin_force 0.0 0.0 0 lin_mode 1 ball attribute contactforce multiply 0.0 contactmoment multiply 0.0 wall attribute zvel −0.1 rang id 1000 1002 wall hist id 30 contactforce id 1000 wall hist id 40 zdisp id 1000 measure creat id 20 x 0 y 0 z 0.08 rad 0.05 measure hist id 200 coord id 20 measure hist id 210 poro id 20 measure hist id 220 stressxx id 20 measure hist id 230 stresszz id 20 cyc 90000 save S1_4.p3sav

Data Availability

The data used to support the findings of this study are available from the corresponding author upon request.

Conflicts of Interest

The authors declare that they have no conflicts of interest.

Acknowledgments

This work was supported by the Department of Science and Technology of Shaanxi Province (nos. 2016ZDJC-24 and 2017KCT-13), China Postdoctoral Science Foundation (nos. 2017M620434), the Special Fund for Basic Scientific Research of Central College of Chang’an University (nos. 310821153502 and 310821173501), and International Cooperation Project of Jiangsu Province (no. BZ 2017011). The authors gratefully acknowledge their financial support.

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