Effects of lime , vermicompost , and chemical P fertilizer on selected properties of acid soils at Ebantu District , Western highlands of Ethiopia

Soil acidity is one of the major factors limiting soil fertility and crop production in large areas of Ethiopia. A two-month incubation experiment was conducted to evaluate the effects of lime, vermicompost (VC), and chemical phosphorus (P) fertilizer on selected chemical properties of Dystric Nitisols in Ebantu District, Western Ethiopia. The treatments comprised of three rates of lime (2, 4, and 6 tons CaCO3·ha−1), VC (2.5, 5, and 7.5 tons·ha−1), and mineral P fertilizer (20, 40, and 60 kg·P·ha−1) each applied alone and in various combinations. The experiment was laid down in a completely randomized design with two replications. The results showed that the highest increment of pH from 4.83 at the control to 6.05 and reduction of exchangeable Al from 1.70 to 0.09 cmolc·kg−1 were obtained from combined application of lime at 4 tons CaCO3·ha−1 and VC at 7.5 tons·ha−1. The most significant decrease in exchangeable acidity (0.17 cmolc.kg-1) was observed in soil that was treated with 6 tons CaCO3·ha−1 lime applied alone (93%) and combined application of lime at 4 tons CaCO3·ha−1 with VC at 7.5 tons·ha−1 by (81%). The highest contents of OM (4.1%) and total nitrogen (0.29%) were obtained from combined application of lime at 4 tons CaCO3·ha−1 and VC at 7.5 tons·ha−1. Integrated application of chemical P (60 kg·P·ha−1) with lime (2 tons·ha−1) plus VC (7.5 tons·ha−1) resulted in Bray-II P increased by 45% relative to control. The various combinations of the treatments also improved exchangeable Ca2+ and Mg2+. The results indicate that integrated use of lime, vermicompost, and chemical P fertilizer can improve soil acidity and availability of nutrients. However, the real potential of the amendments used in this experiment should be further assessed under field conditions using a test crop.


Introduction
Soil acidity is a widespread limitation to crop production in many parts of the world [1].It is a major constraint to agricultural productivity throughout Africa where high rainfall is common due to the deficiencies of nitrogen (N) by leaching, phosphorus (P) by fixation, and low soil organic matter (OM) [2][3][4].As indicated by Schlede [5], World Bank [6], and Wassie and Shiferaw [7], acidic soils cover a significant part of soils of Ethiopia.Hence, it is a serious threat to crop production in most highlands and a major crop production constraint in the small-scale farmers of the country.Specifically because of the severity of soil acidity problem, many crops give a very low productivity in the study district.
Based on the problem that soil acidity causes on a larger areas in Ethiopia, it needs due attention to be addressed by different coping mechanisms [8].e productivity of crops in acid soils with Al toxicity and low soil availability of P may be improved by use of lime, fertilizers with liming effects, and/or organic materials [9,10].Lime is the most effective means of amending soil acidity [2,11].Application of lime containing Ca and/or Mg compounds to acid soil increases Ca 2+ and/or Mg 2+ ions and reduces Al 3+ , H + , Mn 2+ , and Fe 2+ ions in the soil solution.Hence, this leads to increased soil pH and available P due to reduction in P sorption [1,3].Increasing soil pH liming makes other nutrients more available and prevents Al and Mn from being toxic to plant growth [12].Liming also enhances root development and water and nutrient uptakes necessary for healthy plant growth [1,11].
e Ethiopian soils, similar to the other agricultural soils of the tropics, are generally low in P [13,14], and hence, P is one of the limiting elements in crop production in the highlands of Ethiopia.Use of mineral P fertilizers increases the soil available P in P-deficient tropical acid soils [11,15].Melese et al. [16] also suggested that application of the mineral P fertilizer with other amendments can be used to improve P deficiency in acid soils.Even though the chemical fertilizers including mineral P are used to increase productivity for a certain time, their negative impacts coupled with their high cost have prompted the interest in the use of organic fertilizers as source of nutrients.
Organic fertilizer application has been reported to improve crop growth by supplying plant nutrients as well as improving soil physical, chemical, and biological properties [17].Vermicompost (VC) is one of the stabilized, finely divided organic fertilizers with a low C: N ratio, high porosity, and high water-holding capacity, in which most nutrients are present in forms that are readily available for plants [18,19].
ere is an increasing interest in the potential use of VC as soil amendment [20][21][22].Application of VC showed marked improvements in the overall physical and biochemical properties, and at the same time, VC decreases exchangeable acidity which can support a release of plant nutrients in the acidic soils [23].Current trends in agriculture are centered on reducing the use of inorganic fertilizers by biofertilizers such as VC [24].
ere is good evidence that VC application promotes growth of plants and positive effect on growth and productivity of cereals and legumes [20,25,26].When it is compared with conventional compost, VC promotes growth from 50 to 100% over conventional compost and from 30 to 40% over chemical fertilizers [27].
e combined application of inorganic and organic fertilizers is widely recognized as a way of improving productivity of the soil sustainably [28].Several researchers [28][29][30][31] have demonstrated the beneficial effect of integrated nutrient management in mitigating the deficiency of several macro-and micronutrients.
Many parts of the Ethiopian highlands have a problem of acidity which causes the gradual reduction of soil fertility and crop productivity.Almost no research has been done on the effect of VC individually and combined with lime and inorganic fertilizers in ameliorating the acidic soils of the country in general and the study area in particular, except few studies conducted on amendments of acidic soils by lime and lime with other organic and inorganic fertilizers other than VC in different areas [16,[32][33][34].
Yet, most researches just focus on the effect of different ameliorating material on soil acidity.Indeed, no work has been done in ameliorating acidic soils and improving nutrient deficiency by the individual and combined applications of lime, VC, and mineral P. erefore, the objective of this study was to evaluate the effects of lime, VC, and mineral P fertilizers in ameliorating soil acidity-related problems and other selected chemical properties on acidic soils of Ebantu District, Western highlands of Ethiopia.

Description of the Study Area and Sample Collection.
e study was conducted in Ebantu District, East Wollega Zone of Oromia National Regional State (ONRS) (Figure 1).It is located in the western part of Ethiopia at approximately 483 km from Addis Ababa and around 153 km from Nekemte, the capital city of East Wollega zone.e district lies between 9 °58′30″ to 10 °14′0″ N latitude and 36 °3′0″ to 36 °29′0″ E longitude and covers an estimated area of 929 km 2 with an altitude that ranges from 1994 to 2176 meters above sea level (masl).
Geologically, the study area is covered by the metamorphic basement rocks in which tertiary volcanic rocks buildup and that is characterized by fine granular rock, small crystal which is invisible by necked eye. is rock is characterized by large vesicles from where gas escaped out and used for percolation of precipitation [35].e predominant soil type in southwest and western Ethiopia in general and the study area in particular is Dystric Nitisols according to [36] the soil classification system.Its vernacular name is "Biyyee Diimaa" meaning red soil.On the average, the soil is deep and relatively highly weathered, well drained, and very strongly to strongly acidic in reaction.Nitisols are highly weathered soils in the warm and humid areas of the west and southwest Ethiopia [37].
In terms of topography, 30% of the total area is gentle slope, while flat and steep slope lands account for 52 and 18%, respectively.Out of the total area of the district, 35% is covered by cultivated land, 19% by grazing land, 20% by natural forest land, 16% by fallow land, and 8% by shrubs, and about 2% is estimated to be area covered by settlement (Ebantu District Agricultural development Bureau, 2014 unpublished).e natural forest in the study area consists of some tree species that are remnants of a once dense evergreen forest occurring in various areas of the district.e dominant tree species in the area include Acacia etbaica, Acacia abyssinica, Cordia africana, Syzygium guineense, Ficus sur, Albizia julibrissin, Eucalyptus sp., Croton macrostachyus, and Podocarpus falcatus (personal observation).
According to the local and the Ethiopian agroclimatic zonation [38], the study area belongs to the humid (Baddaa) and subhumid (Badda Daree) climatic zones.e economic activities of the local society of the study area are primarily mixed farming system that involves animal husbandry and crop production.Continuous cultivation without any fallow periods coupled with complete removal of crop residues is a common practice on cultivated fields.Farmers in the study area use diammonium phosphate (DAP), urea, and cow dung as sources of fertilizers.e major crops are maize (Zea mays L.), teff (Eragrostis tef), coffee (Coffee arabica L.), barley (Hordeum vulgare L.), potato (Solanum tuberosum L.), and noug (Guizotia abyssinica).ese major crops are produced usually once per year.

2
Applied and Environmental Soil Science e district receives an annual average rainfall of 1778 mm and has monthly mean minimum, maximum, and mean air temperatures of 16.6, 20, and 18.3 °C, respectively [39] (Figure 2).e rainfall pattern is unimodal, stretching from April to October.
A bulk soil sample was taken from the surface soil (0-20 cm) from the very strongly acidic soil of the Walgayi Soruma sampling site in Ebantu District, Western Highlands of Ethiopia.Totally, three composite samples were collected from the three blocks.Soil samples were collected by auger from eighteen subsamples in each block and thoroughly mixed to make a composite.e soil was air-dried, ground, and passed through a 2 mm and 0.5 mm sieve and analyzed for selected soil physicochemical properties.At the same time, a total of 3 undisturbed soil samples at 0-20 cm depth layer to determine soil bulk density (BD) of the area were collected in random by taking one sample per block using the core method.All the laboratory activities were undertaken at Haramaya University and the Nekemte Soil Research Center.

Set Up of the Incubation Experiment.
e incubation experiment was conducted for two months as described below.e composite soil sample with three replicates was air-dried, ground, and passed with 2 mm sieve, and then 0.3 kg soil was placed in plastic pot and mixed with di erent treatments in a greenhouse.During incubation, soil  moisture was adjusted to a constant weight 60% ( eld capacity) with distilled water at the end of every 3-day period.
In this experiment, lime (CaCO 3 ) at rates of 2, 4, and 6 tons•ha −1 (corresponding with 0.231, 0.462, and 0.693 g/ 0.3 kg soil, resp.), based on the results from LR tests to reach desired pH values, three VC rates (2.5, 5 and 7.5 tons•ha −1 ), and three mineral P fertilizer rates (20, 40, and 60 kg•P•ha −1 ), as triple superphosphate (Ca(H 2 PO 4 ) 2 ), were separately applied uniformly to the whole soil volume.e lime rate (4 tons•ha −1 ) was combined separately with each of VC and mineral P fertilizer rates as treatments, VC rate (5 tons•ha −1 ) was combined separately with each of mineral P fertilizer rate and di erent rates of lime, and VC and mineral P were combined and applied to the soil as additional ve treatments.A control treatment with no soil amendments was used for the incubation experiment.A total of 48 pots were used for the incubation experiment.e experiment was laid down in a completely randomized design (CRD) with two replications.e units of the treatments were converted into hectare bases by assuming that the plough depth is 20 cm and ρ b of the soil is 1.3 g•cm −3 .e soils were incubated with the treatments in the pots for two months (November and December, 2014) at Haramaya University main campus (rare).
Soil samples were taken at the end of the incubation time, air-dried, ground, and sieved through 2 mm and 0.5 mm sieve to observe the e ects of lime, VC and mineral P individually and in combined form on selected soil acidity related and other soil chemical properties at Haramaya University central and soil chemistry laboratory.

Soil Analyses.
Soil particle size distribution was analyzed by the Bouyoucus hydrometer method [40] after the soil samples were dispersed with sodium hexametaphosphate [(NaPO 3 ) 6 ].Soil bulk density (ρ b ) was measured from three undisturbed soil samples collected using a core sampler (2.5 cm radius and 5.0 cm height) as per the procedure described by Jamison et al. [41] while particle density (ρ s ) was measured using the pycnometer [42] at the Nekemte Soil Research Center.Total porosity (φ) was calculated from the values of ρ b and ρ s as follows: Soil pH was measured potentiometrically in 1 : 2.5 soil : H 2 O suspension using a combined glass electrode pH meter [43].Total exchangeable acidity was determined by saturating the soil samples with 1 M•KCl suspension as described by [44].From the same extract, exchangeable Al in the soil samples was determined by application of 1 M•NaF which forms a complex with Al and releases NaOH.Acid saturation (AS) was calculated as follows: where AS refers to acid saturation and ECEC refers to effective cation exchange capacity.Organic carbon (OC) content of the soil was determined by the wet combustion procedure of Walkley and Black [45].Organic matter was determined by multiplying OC by 1.724 factors.e total nitrogen (N) content of the soil was determined by wet-oxidation procedure of the Kjeldahl method [46].Available P was extracted by the Bray-II method [47] using 0.03 M•NH 4 F and 0.1 M•HCl solution.
Exchangeable basic cations (Ca, Mg, K and Na) were determined by saturating several times the soil samples with 1 M•NH 4 OAc solution at pH 7.0.
en Ca and Mg were determined by using atomic absorption spectrophotometry (AAS), while exchangeable Na and K were measured by ame photometer from the same extract [48].e e ective cation exchange capacity (ECEC) was calculated as the sum of exchangeable acidity (Al 3+ and H + ) and exchangeable basic cations (Ca 2+ , Mg 2+ , K + , and Na + ) [49].

Vermicompost and Lime Analyses.
e VC was prepared from decomposition of cow dung, sheep and goat manures, crop and home residues, and weeds and grasses by using red earthworm (Eisenia fetida).Selected parameters of VC were determined using dried samples which were ground to pass through a 2 mm sieve as described by Pisa and Wuta [51].Electrical conductivity (EC) and pH were determined from a suspension of 1 : 10 VC : H 2 O as described by Ndegwa and ompson [52].e total OC was estimated by the wet digestion and rapid titration method [45].
e total N content of the VC was determined by wet-oxidation procedure of the Kjeldahl method [46].Total Ca, Mg, K, and Na were extracted by wet digestion using concentrated sulphuric acid (H 2 SO 4 ), selenium (Se) powder, lithium sulphate (Li 2 SO 4 ), and hydrogen peroxide (H 2 O 2 ) mixture [53].Total Ca and Mg were determined from the wet digested samples by AAS while K and Na were estimated by ame photometer.Total P was extracted using concentrated H 2 SO 4 , Se  [53].e calcium carbonate equivalent (CCE) of the Guder lime was determined by dissolving the lime using excess of standard 0.5 M•HCl and followed by gentle boiling.After filtration, the excess HCl was back titrated with standard 0.1 M•NaOH solution.From the amount of NaOH used to neutralize the excess acid of the blank and the filtrate, the CCE value of the lime was calculated [50].
Lime requirement was determined by the acid saturation method to ameliorate the acidic soil of the study site for the maize crop.e acid saturation method uses exchangeable acidity, ECEC, and permissible acid saturation percentage of crops to calculate the amount of lime to be applied.Using the acid saturation method, lime requirement is calculated as follows [54]: where LR � lime requirement, LRF � lime requirement factor (kg•lime•ha −1 ) to lower the Ex.acidity by 1 cmol (3000 kg lime/ha/cmole) [55] for most Ethiopian soils, Ex. acidity � exchangeable acidity (Al 3+ + H + ), PAS � permissible acid saturation, and ECEC � effective cation exchange capacity (exchangeable acidity + exchangeable bases).

Statistical Analysis. Analysis of variance was carried out
on the effect of treatments on selected soil chemical properties using SAS software [56].Duncan's multiple range test was employed to test the significance difference between means of treatments.Simple Pearson correlation analysis was executed to determine the associations between various soil acidity parameters and different soil chemical properties.

Initial Soil Properties and Vermicompost Composition.
e results of laboratory analysis of selected properties of the soil used for the experiment are presented in Table 1.e textural class of the soil used for the incubation experiment is loam.e bulk density of the soil was below the critical value of bulk density (1.6 gcm −3 ) for plant growth at which root penetration is likely to be severely restricted in a loam soil [57], while the particle density is lower than the average particle density value for a mineral soil.Due to the low bulk density value, the total porosity of the soil was relatively high.e soil was strongly acidic [57] with relatively high content of exchangeable acidity and Al. e percentage acid saturation of the soil was 30.7%. e organic matter and total nitrogen contents of the soil were in the range of low and moderate, respectively [58], while the available P content was in the low range [59].Similarly, the mean soil exchangeable Ca and K were low, whereas exchangeable Mg was within the range of medium [60].e effective CEC of the soils was also relatively low probably due to the dominance of low activity clay minerals in the highly weathered soils of the study area.As per rating suggested by Jones [57], the soil was high in DTPA-extractable Fe, Mn, and Zn and medium in extractable Cu [57].In general, the results of the soil preanalysis clearly indicate that the soil has soil fertility problems that include deficiency of major plant nutrients and soil acidity that limit successful production of crops in the study area. is calls for development of appropriate management practices that enhance crop production on a sustainable basis.
e lime used in this study had CCE value of 88.7%.Table 2 shows the nutrient contents of the vermicompost used for the experiment.
e nutrients are likely to be  derived from decomposition of the organic matter by the activities of microorganisms.e contents of the VC could decrease soil acidity and enhance soil fertility in the strongly acidic soils of the study area. is is manifested by the high pH of the compost.In line with the findings of this study, Wael et al. [61] stated that VC was used to increase the pH in acidic soils and reduce Al and Mn toxicity because of its alkalinity.Arancon et al. [18] and Asciutto et al. [62] also reported that VC contains most nutrients, such as exchangeable Ca, phosphates, and soluble K in plant available forms.

Effects of Treatments on pH, Exchangeable Acidity and Al, and Acid
Saturation. e lime at each respective application level alone or in combination with VC had significant (P ≤ 0.001) effects on soil pH, exchangeable acidity and Al, and acid saturation (AS) (Table 3).e highest lime rate (6 tons•CaCO 3 •ha −1 ) significantly (P ≤ 0.001) increased the pH from 4.80 to 6.01, reduced both the exchangeable acidity and Al from 2.4 to 0.17 cmol c •kg −1 and 1.70 to 0.33 cmol c •kg −1 , respectively, and reduced acid saturation from 30% to 1.62%. is might be because lime contains Ca 2+ cation to exchange and/or replace H + ion on the exchange sites and anions such as CO 3  2-to neutralize the H + ion released from the exchange sites and hydrolyzing Al species to the soil solution.In consent with the results of this study, Kisinyo et al. [63] and Kisinyo et al. [15] reported that application of lime to acid soils increased Ca 2+ and/or Mg 2+ ions and reduced Al 3+ , H + , Mn 2+ , and Fe 2+ ions in the soil solution.
Vermicompost at each respective application levels had also significant (P ≤ 0.001) effects on the soil pH, exchangeable acidity and Al, and AS (Table 3).e rise in soil pH due to application of VC might be attributed to its high content of basic cations and pH, which could reduce soil acidity and the contents of exchangeable acidity and Al through replacing the acidic cations from the exchange sites.
is is in agreement with the findings of Angelova et al. [64] who pointed out that the direction of the change in soil pH as a result of VC application reflected the initial pH of VC.
Generally, the combination of all lime-VC treatments significantly (P ≤ 0.001) increased soil pH and decreased exchangeable acidity and Al relative to the control (Table 3).
Combination of the highest level of VC (7.5 tons•ha −1 ) with  3).When lime at a rate of 4 tons•ha −1 was applied to the soil in combination with VC at the rate of 5 and 7.5 tons•ha −1 , the soil pH increased to the optimum pH for many crops.e correlation analysis also indicated that the pH of the soils was correlated with the exchangeable acidity (r � −0.87 * * , P ≤ 0.01), exchangeable Al (r � −0.87 * * , P ≤ 0.01), and AS (r � −0.88 * * , P ≤ 0.01) (Table 4). is is in agreement with Opala et al. [65] who indicated that the combination of organic fertilizers having liming effect and inorganic fertilizers decreased exchangeable acidity which in turn increased soil pH. e chemical P fertilizer had no significant (P ≤ 0.001) effect on soil pH, exchangeable acidity and Al, and AS when applied alone over the control (Table 3).Along with this, Kisinyo et al. [63] reported that application of the P fertilizer alone to acidic soils did not increase the soil pH neither reduced soil exchangeable acidity.

Effects of Treatments on Organic Matter, Total Nitrogen, and Available
Phosphorus.Compared to the control, all the other treatments showed significant (P ≤ 0.001) increase in soil OM except all levels of chemical P and the combination of lime (4 tons CaCO 3 •ha −1 ) with all levels of the chemical P fertilizer (Table 5).e highest content of OM (4.1%) was obtained when the soil was treated by the combination of lime (4 tons CaCO 3 •ha −1 ) with highest level of VC (7.5 tons•ha −1 ) (Table 5).Lime and VC application either individually or in combination increased soil pH and OM content, which in turn enhances the microbial population.An increase in pH may decrease the stress on soil microbes and microbial activity and thus increases soil OM. is is supported by the correlation in which pH was positively and significantly (r � 0.55 * * ; P ≤ 0.01) correlated with OM (Table 4).In agreement with this, Amba et al. [66] indicated that soil OC increment after the application of lime and manure was associated with the general improvement of soil conditions.e application of treatments significantly (P ≤ 0.001) increased soil total N except the three rates of the chemical P fertilizer alone (Table 5).e application of OM in the form of VC is expected to increase the OM and TN contents of the soil. is is also evidenced by the total by the positive and significant correlation between total N (r � 0.89 * * ; P ≤ 0.01) and OM (Table 4). is is in agreement with Adeleye et al. [67] and Efthimiadou et al. [68] who stated that soil total N increases when biofertilizers are solely applied due to the addition of OM. Mary and Sivagami [69] also reported that VC is rich in total N. e highest increment of total N (0.29 mg•kg −1 ) was obtained when lime (4 tons•ha −1 ) was applied in combination with VC (7.5 tons•ha −1 ) (Table 5).Similar to the results of the current study, Biruk et al. [34] reported increase in total N in acidic soils treated with lime and compost.
erefore, the application of the treatments at these rates significantly (P ≤ 0.0001) increased available P by 45% over the control (Table 5). is might be due to the significant (P ≤ 0.001) increase in soil pH due to the effect of lime and VC, which in turn reduced P fixation.is is also supported by the results of the simple correlation analysis which indicated that the available P of the soil was positively and significantly correlated to the pH (r � 0.69 * * , P ≤ 0.01) (Table 4). is is in harmony with the findings of Anetor and Akinrinde [70] who indicated that increase in soil pH due to lime application reduced P fixation.Similarly, Kisinyo et al. [63] reported that the application of lime and chemical P fertilizer in sole or combination had significantly positive effect on soil pH and available P in acid soils.Application of the P fertilizer increased available P due to increase of P in soil.Similar increase in soil available P in tropical soils has been reported by Kisinyo et al. [15] and Opala et al. [4].Combined application of chemical P and VC increased soil available P more than when either of them were applied alone.is was because the organic material reduced soil P sorption making both the soil native P and the applied P fertilizer available for plant uptake.Similar results were reported by Kisinyo [71] and Opala et al. [72].

Effects of Treatments on Exchangeable Bases and
Effective Cation Exchange Capacity.Soil exchangeable Ca was significantly (P ≤ 0.001) increased by the application of Applied and Environmental Soil Science all treatments except the application of chemical P alone (Table 6).
e highest (7.7 cmol c •kg −1 ) and lowest (3.5 cmol c •kg −1 ) soil exchangeable Ca was obtained when the soil was treated by VC (7.5 tons•ha −1 ) plus lime (4 tons•ha −1 ) and chemical P (40 kg•ha −1 ), respectively, relative to the control (Table 6).Furthermore, lime and VC when applied separately increased soil exchangeable Ca over the control (Table 6).e increase in exchangeable Ca due to the combined use of lime and VC could be associated with the release of Ca 2+ from the applied lime through its dissolution and vermicompost, which replaces the acidic cations from the exchange site.erefore, the most effective and significant increase was observed when VC was combined with lime plus the chemical P fertilizer. is is in agreement with the previous works of Hassen et al. [73] and Adeleye et al. [67] who reported increase in exchangeable Ca following combined application of lime and organic fertilizers.
Soil exchangeable Mg was also significantly (P ≤ 0.001) increased as a result of the treatments applied except the chemical P fertilizer (Table 6).Accordingly, the highest exchangeable Mg (3.44 cmol c •kg −1 ) was recorded from in the application of lime (4 tons•ha −1 ) with VC (7.5 tons•ha −1 ) (Table 6).e increased soil exchangeable Mg as a result of lime and VC application might be attributed to increase in soil pH which in turn may have increased Mg availability in the soil.When VC was combined with lime and chemical P fertilizer, soil exchangeable Mg was increased, and this was attributed to addition of nutrients to the soil from the VC.In addition, the increase of soil pH by VC reduces Al 3+ and H + content in soil exchange sites and then increased Mg availability.
e results are in agreement with those of Repsiene and Skuodiene [74] and Andric et al. [75] who reported that soil exchangeable bases increased when acidic soil was amended by lime and manure.
e increase in soil exchangeable K and Na due to application of VC alone or in combination with the P fertilizer plus lime could be due to added K and Na from VC.
e VC used in the current study had 27.7 and 14.2 cmol c •kg −1 of K and Na contents, respectively, which might have added significant amounts of these nutrients to the soil (Table 2). is is supported by the report of Ayeni and Adetunji [76], Adeleye et al. [67], and Adeniyan et al. [77] who indicated that soil exchangeable bases increase when the biofertilizer was applied alone or in combination with the lime and P fertilizer.
e effective cation exchange capacity (ECEC) of the soil was significantly (P ≤ 0.001) affected by all treatments except the chemical P fertilizer when applied at the rate of 40 and 60 kg•P•ha −1 (Table 6). is increase was due to improved soil conditions such as soil pH, increased soil Ca, Mg, K, and Na by VC and lime and increase of negative charges on the surfaces of the soil colloids following the rise in pH. e ECEC increment might also be caused by deprotonation of pH-dependent charge sites arising from VC. is is in agreement with the findings of Edmeades [78] who stated that ECEC increased with increasing pH of soils.e ECEC was significantly increased with the increase of VC due to the greater contents of exchangeable bases of VC. is is supported by Pandey and Shukla [79] who indicated that application of VC changed ECEC of the soil due to the change of negative surfaces of the soil colloids.

Effects of Treatments on Extractable Micronutrients (Fe, Mn, Zn, and Cu).
e extractable micronutrients were significantly (P ≤ 0.001) affected by treatments (Table 7).Under almost all the treatments, all extractable micronutrients decreased relative to the control (Table 7).e extractability of Fe, Mn, Zn, and Cu tends to decrease as soil pH increased.
e exact mechanisms responsible for reducing availability differ for each nutrient, but can include formation of low solubility compounds, greater retention by soil colloids when lime and VC are applied.e decrease in extractable Fe may be due to the change in pH caused by the amendments because the bioavailability of DTPA-extractable Fe was decreased when pH of the soil increased.In consent with this, Imerb et al. [80] and Wael et al. [61] reported that extractable Fe decreased at pH levels near neutral or higher.
e application of lime and VC decreased extractable Mn as compared with the control.is might be due to high CEC of organic fertilizer and its ability to form chelate complexes with this nutrient.Along with this, Angelova et al. [64] reported that the application of amendments decreased the extractable Mn concentration in the soil which might be due to immobilization of Mn by the application of VC.Extractable Zn was decreased significantly (P ≤ 0.001) by the application of lime and VC and also in combination of all treatments. is may be due to the increment of soil pH and also the formation of insoluble form of Zn compound when it reacts with VC. is in agreement with Walker et al. [81] who pointed out that Zn availability is controlled by soil pH.Angelova et al. [64] also indicated that Zn can form insoluble compound precipitates during the mineralization of organic ameliorants.e extractable Cu was decreased by the application of amendments.Especially, VC supplements lead to lower content of DTPA-extractable Cu. is may be due to the transformation of OM in stable form that could link more Cu.In concord to this, Angelova et al. [64] reported that enrichment of soil with OM could reduce the bioavailable Cu as a result of complexation of free ions of Cu.

Conclusion
e study revealed that soils of the study area have limitations related to deficiency of major plant nutrient elements and soil acidity.As a result, most of the soil properties measured responded positively to applications of lime, VC, and chemical P fertilizer either in combination or alone.is incubation experiment demonstrated that the application of lime, VC, and chemical P fertilizer could mitigate soil acidity and Al toxicity as well as improve soil fertility of acidic soils of the study area.e combined application of medium rates of lime (4 tons•ha −1 ), VC (5 tons•ha −1 ), and chemical P (40 kg•ha −1 ) holds a lot of promise as an efficient alternative to amend soil acidity and increase soil nutrient availability.However, the results need to be confirmed under field conditions, and the economic feasibility of application of a particular combination needs to be quantified.erefore, further field work is recommended to verify this result.

Table 1 :
Selected physical and chemical properties of the experimental soil before incubation.

Table 3 :
Effects of the treatments on pH, exchangeable acidity and exchangeable Al, and percent acid saturation of soil in the incubation study.

Table 5 :
Effects of treatments on organic matter, total nitrogen, and available phosphorus of the soil after incubation.

Table 6 :
Effects of treatments on exchangeable bases and effective cation exchange capacity.

Table 7 :
e effects of treatments on extractable micronutrients (Fe, Mn, Zn, and Cu) of the soil of the study area.