Anatomy Research InternationalVolume 2013 (2013), Article ID 254250, 14 pageshttp://dx.doi.org/10.1155/2013/254250

Review Article

Root Anatomy and Root Canal Configuration of Human Permanent Mandibular Premolars: A Systematic Review

Jojo Kottoor,¹ Denzil Albuquerque,² Natanasabapathy Velmurugan,³ and Jacob Kuruvilla⁴

¹Department of Conservative Dentistry and Endodontics, Mar Baselios Dental College, Kothamangalam, Ernakulam, Kerala 686691, India²Endodontic Speciality Practice, Dental Expert, Orlem, Malad, Mumbai 400064, India³Department of Conservative Dentistry and Endodontics, Meenakshi Ammal Dental College and Hospital, Alapakkam Main Road, Maduravoyal, Chennai 600 095, Tamil Nadu, India⁴Department of Public Health Dentistry, Mar Baselios Dental College, Kothamangalam, Ernakulam, Kerala 686691, India

Received 31 July 2013; Revised 4 November 2013; Accepted 5 November 2013

Academic Editor: David Tai Wai Yew

Copyright © 2013 Jojo Kottoor et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract.

Introduction. Mandibular premolars have been reported with complex anatomical aberrations, making them one of the most difficult teeth to manage endodontically. Methodology. An exhaustive search was undertaken to identify associated anatomic studies of mandibular premolars through MEDLINE/PubMed database using keywords, and a systematic review of the relevant articles was performed. Chi-square test with Yates correction was performed to assess the statistical significance of any anatomic variations between ethnicities and within populations of the same ethnicity. Documented case reports of variations in mandibular premolar anatomy were also identified and reviewed. Results. Thirty-six anatomic studies were analyzed which included 12,752 first premolars and nineteen studies assessing 6646 second premolars. A significant variation in the number of roots, root canals, and apical foramen was observed between Caucasian, Indian, Mongoloid, and Middle Eastern ethnicities.The most common anatomic variation was C-shaped canals in mandibular first premolars with highest incidence in Mongoloid populations (upto 24%) while dens invaginatus was the most common developmental anomaly. Conclusions. A systematic review of mandibular premolars based on ethnicity and geographic clusters offered enhanced analysis of the prevalence of number of roots and canals, their canal configuration, and other related anatomy.

1. Introduction

A clear understanding of the anatomy of human teeth is an essential prerequisite to all dental procedures especially so in the case of root canal treatment which deals with management of the tooth’s internal anatomy. The pulp space is divided into two parts: the pulp chamber, which is usually described as that portion within the crown, and the pulp canal or root canal, which lies within the confines of the root. The pulp chamber is a single cavity; the dimensions of which vary according to the outline of the crown and the structure of the roots. In multirooted teeth the depth of the pulp chamber depends upon the position of the root furcation and may extend beyond the anatomical crown [1].

The pulp space is complex; root canals may divide and rejoin, and possess forms that are considerably more involved than commonly implied. Many roots have additional canals and a variety of canal configurations. In the simplest form, each root has a single canal and a single apical foramen (Type I). Commonly, however, other canal complexities are present and exit the root as one, two, or more apical canals (Types II–VIII) [2]. This could be better understood through an insight into the development of root formation. At a more advanced stage of tooth development, when enamel and dentin formation has reached the future cementoenamel junction, the dental root begins to form from a cellular diaphragm or horizontal Hertwig’s epithelial root sheath. The horizontal Hertwig’s epithelial root sheath may vary in shape, depending on whether the teeth are single- or multirooted. In fact, its shape determines the number of roots in a tooth. If the diaphragm remains in the shape of a collar, a single-rooted tooth will form. On the other hand, if two or three tongues of epithelium grow towards each other from this collar to bridge the gap and fuse, two or three diaphragms evolving independently from each other will form. They will either remain fused, forming fused roots, or single roots with multiple canals, or separated, forming distinct roots in multirooted teeth [3].

Mandibular premolars typically present with a single root and a single canal. The solitary root is usually oval in cross section containing an oval cross-section canal. Canal configurations in mandibular premolars may vary significantly with respect to ethnicity, race, and sex [2]. The purpose of this paper was to perform a systematic review of the literature related to the root anatomy and root canal configuration of the permanent mandibular first and second premolars.

2. Materials and Methods

2.1. Literature Search and Data Extraction

An exhaustive search was undertaken through MEDLINE/PubMed database to identify published literature related to the root anatomy and root canal morphology of the permanent mandibular premolars by using key words “root canal anatomy,” “root canal morphology,” “mandibular premolars,” “mandibular first premolar,” and “mandibular second premolar,” alone or in combination. Related anatomic studies of human permanent mandibular premolars were identified and a literature review was performed for articles dated July 2013 and before. The data was analyzed according to the population ethnicity and demography, number of teeth per study (power), number of roots, number of root canals, method of tooth analysis, root canal patterns, and number of apical foramina. Additionally, documented case reports of anatomic variations and developmental anomalies were identified and reviewed. Statistical comparisons were done between ethnicities and within populations of particular ethnicities using Chi-square test with Yates correction to allow for better understanding of the variations and the statistical significance of these variations in radicular anatomy of mandibular premolars based on the data collected through the systemic review.

3. Results

Thirty-six anatomic studies were analyzed which included 12,752 first premolars and nineteen studies of 6646 second premolars. A summary of the findings of different anatomic studies based on their ethnicity or geographical population assessment, with regard to the number of roots and canals has been tabulated for mandibular first premolars (Table 1) [4–38] and second premolars (Table 2) [4–7, 9, 11, 12, 15, 19, 21, 25, 29, 31–33, 35–40].

Table 1: Summary of the incidence of documented ethnic and population variations in the anatomy of mandibular first premolars.

Geographic and ethnicdistribution
Materials and methods
No. of teeth in the study ()
Root anatomy
Root canal anatomy
Root canal configuration (Vertucci)
Apical foramina
References

1 root
2 roots
>2 roots
1 canal
2 canals
>2 canals
C-shaped
Type I
Type II
Type III
Type IV
Type V
Type VI
Type VII
Type VIII
Others types
1
2
3

Caucasian

France
In vitro: radiograph and sectioning
341
90.6% (309)
6.4% (22)
3% (10)
68.9% (235)
31.1% (106)
0% (0)
0% (0)

Geider et al. [4], 1989

Germany
In vitro: examination
2369
99.3% (2352)
0.7% (17)
0% (0)

Schulze. [5], 1970

Poland
In vitro: extraction and RCT
83

89.3% (74)
10.7% (9)
0% (0)
0% (0)

Rózyło et al. [6], 2008

USA
In vitro: clearing
400
100% (400)
0% (0)
0% (0)
70% (280)
25% (100)
5% (20)
0% (0)
70% (280)
0% (0)
4% (16)
1.5% (6)
24% (96)
0% (0)
0% (0)
0% (0)
0% (0)
74% (296)
26% (104)
0% (0)
Vertucci [7], 1894

In vivo: radiographic examination
1002

81.8% (820)
18.2% (182)
0% (0)
0% (0)

Sabala et al. [8], 1994

In vitro: grinding and examination under magnification
50

86% (43)
14%(7)
0% (0)
0% (0)

90% (45)
10% (5)
0% (0)
Green [9], 1973

In vitro: sectioning
106

74% (78)
26% (28)
0% (0)
14%(15)
76% (80)
0% (0)
0% (0)
24% (26)
0% (0)
0% (0)
0% (0)
0% (0)
0% (0)
76% (80)
24% (26)
0% (0)
Baisden et al. [10], 1992

In vitro: sectioning
32
100% (32)
0% (0)
0% (0)
35.5% (12)
62.5% (20)
0% (0)
0% (0)

Barrett. [11], 1925

Extraction and radiograph
1287

66.9%(861)
32.7%(421)
0.4% (5)
0% (0)

80.7% (1039)
19.3% (248)
0% (0)
Zillich and Dowson [12], 1973

In vitro: radiographs
156

95.5% (149)
4.5%(7)
0% (0)
0% (0)

Mueller et al. [13], 1933

In vivo: radiographic examination
400
94.5% (378)
5.5% (22)
0% (0)
86.3% (345)
13.7% (55)
0% (0)
0% (0)

Trope et al. [14], 1986

Subgroup Ethnic Incidence

6226
96.88%  (3471)
2.52%  (61)
0.6%  (10)
75.42%  (2586)
23.84%  (935)
0.54%  (25)
1.4%  (15)
73%  (360)

2%  (16)
12.75  (32)
12%  (96)

80%  (1490)
20%  (383)

African

African American
In vivo: radiographic examination
400
83.8% (335)
16.2% (65)
0% (0)
67.2% (269)
32.8% (131)
0% (0)
0% (0)

Zillich and Dowson [12], 1973

Senegalese
In vivo: radiographic examination
412

81.3% (335)
15.1% (62)
3.6% (15)
0% (0)

Mbaye et al. [15], 2008

Subgroup Ethnic Incidence

812
83.8%  (335)
16.2% (65)

74% (604)
23.95% (193)
1.8% (15)

Indian

India
In vitro: SCT
100

80% (80)
11%(11)
0% (0)
2%(2)
80% (80)
9%(9)
3%(3)
2%(2)
4%(4)
0% (0)
0% (0)
0% (0)
2%(2)
94% (94)
6% (6)
0% (0)
Sandhya et al. [16], 2010

In vivo: radiographic examination
1000
95.9% (959)
3.9% (39)
0.2% (2)

75.4% (754)
1% (10)
0% (0)
20.8% (208)
2.4% (24)
0% (0)
0% (0)
0.4% (4)
0% (0)
76.4% (764)
23.2% (232)
0.4% (4)
Iyer et al. [17], 2006

In vitro: clearing
100

72% (72)
27% (27)
0% (0)
1%(1)
72% (72)
6% (6)
3% (3)
10% (10)
8% (8)
0% (0)
0% (0)
0% (0)
0% (0)
82% (82)
18% (18)
0% (0)
Velmurugan and Sandhya [18], 2009

In vitro: clearing
40

50% (20)
50% (20)
0% (0)
0% (0)
50% (20)
5% (2)
5% (2)
25% (10)
12.5% (5)
2.5% (1)
0% (0)
0% (0)
0% (0)
82% (82)
18% (18)
0% (0)
Parekh et al. [19], 2011

In vitro: clearing
138
97.1% (134)
2.89% (4)
0% (0)
67% (92)
33% (46)
0% (0)
0% (0)
67.4% (92)
8%(11)
3.7% (5)
3.9% (5)
17.4% (24)
0.7% (1)
0% (0)
0% (0)
0% (0)
77.8% (107)
22.2% (31)
0% (0)
Jain and Bahuguna [20], 2011

In vitro: SCT
112
96.4% (108)
3.4% (4)
0% (0)
70.5%(79)
29.5%(33)
0% (0)
10.7%(12)
80%(90)
9%(10)
3%(3)
2%(2)
4%(4)
0% (0)
0% (0)
0% (0)
0% (0)
98% (110)
2%(2)
0% (0)
V. K. Sikri and P. Sikri [21], 1994

Subgroup Ethnic Incidence

1490
96.47%  (1201)
3.4%  (47)
0.07%  (2)
68%  (343)
30%  (137)
0%
3%  (15)
71%  (1108)
6%  (48)
3%  (16)
11%  (237)
8%  (69)
0.53%  (2)

0.06%  (4)
0.33  (2)
85%  (1239)
15%  (307)
0.07%  (4)

Mongoloid

China
In vitro: radiographs
100

64% (64)
34% (34)
2%(2)
0% (0)
78% (78)
6%`(6)
6%(6)
10% (10)
0% (0)
0% (0)
0% (0)
0% (0)
0% (0)
90% (90)
10% (10)
0% (0)
Walker [22], 1988

In vitro: radiograph and sectioning
82

54% (44)
46% (38)
0% (0)
0%(0)

Lu et al. [23], 2006

In vitro: Micro-CT
115

65.2% (75)
26.1% (40)
0% (0)
0% (0)
65.2% (75)
0% (0)
2.6% (3)
0% (0)
22.6% (26)
0% (0)
0.9% (1)
0% (0)
0% (0)
76.5% (88)
23.5% (27)
0% (0)
Liu et al. [24], 2013

In vivo: CBCT
178
98% (174)
2% (4)
0% (0)
87.1% (155)
11.2% (20)
0.6% (1)
1.1%(2)
86.8% (151)
0% (0)
1.7% (3)
0% (0)
9.8% (17)
0% (0)
0% (0)
0.6%(1)
0% (0)
89.6% (160)
9.8% (17)
0.6%(1)
Tian et al. [25], 2012

In vivo: CBCT
97

83.5% (81)
12.4% (12)
0%(0)
4.1%(4)
83.5% (81)
0% (0)
3.6% (3)
0% (0)
8.8% (8)
0% (0)
0% (0)
0% (0)
0% (0)
91.2% (88)
8.8% (9)
0% (0)
Liao et al. [26], 2011

In vivo: CBCT
440
99.5% (439)
0.5% (1)
0% (0)
75.2% (340)
21% (92)
0.7% (3)
1.1%(5)
76% (335)
3.4% (15)
2.7% (12)
6.6% (21)
9.3% (41)
0% (0)
0% (0)
0.7% (3)
0% (0)
83.4% (367)
15.9% (70)
0.7%(3)
Tian et al. [27], 2013

In vitro: Micro-CT
358

24%(86)

Fan et al. [28], 2008

Japan
In vitro: radiographs
516

86.2% (445)
13.8% (71)
0% (0)
0% (0)

Miyoshi et al. [29], 1977

In vitro: Staining
139

80.6% (112)
15.1% (21)
4.3% (6)
0% (0)

80.6% (112)
19.4% (27)
0% (0)
Yoshioka et al. [30], 2004

Korea
In vivo: CBCT
797
99.9% (796)
0.1% (1)
0% (0)

Park et al. [31], 2013

Subgroup Ethnic Incidence

2822
99%  (1409)
1%  (6)

74.5%  (1311)
22.5%  (328)
.95%  (12)
3.7%  (97)
78%  (720)
0.85%  (21)
3%  (27)
3.32%  (31)
10.1%  (92)

0.18%  (1)
0.74%  (4)

85%  (905)
15%  (160)
0.65%  (4)

Middle East

Kuwait
In vivo: radiograph of RCT teeth
20
85.0% (17)
15% (3)
0% (0)
60%(12)
40%(8)
0% (0)
0% (0)

60% (12)
40% (8)
0% (0)
Zaatar et al. [32], 1997

Jordan
In vitro: clearing
500
97% (485)
3% (15)
0% (0)
58.2% (291)
39.8% (195)
2.6% (13)
0% (0)
58.2% (251)
4.8%(24)
1.4%(7)
14.4% (72)
16.8% (84)
0.8%(4)
1%(5)
0% (0)
2.6%(5)

Awawdeh and Al-Qudah [33], 2008

Iran
In vitro: sectioning
217
100% (217)
0% (0)
0% (0)
88.47% (192)
11.53%(25)
0% (0)
1.4%(3)
90%(195)
1.8% (4)
3.2%(7)
0.9% (2)
4.1% (9)
0% (0)
0% (0)
0% (0)
0% (0)
93.8%(203)
5%(11)
1.2%(3)
Khedmat et al. [34], 2010

In vitro: clearing
163
98% (160)
2% (3)
0% (0)
70.6% (115)
27.8% (45)
1.2%(2)
2.4%(4)
70.6% (113)
1.9%(3)
3.8%(6)
3.8% (6)
16.9% (27)
1.2%(2)
0.6%(1)
0% (0)
1.2%(2)
77.5%(126)
22.5%(37)
0% (0)
Rahimi et al. [35], 2007

Turkey
In vitro: clearing
100
100% (100)
0% (0)
0% (0)
64% (64)
30% (30)
5%(6)
0% (0)

75% (75)
25% (25)
0% (0)
Çalişkan et al. [36], 1995

In vitro: clearing
200

60.5% (121)
39.5% (69)
0% (0)
0% (0)
60.6% (121)
18.5% (37)
10.5% (21)
7% (14)
2.5% (5)
0% (0)
0% (0)
1% (2)
0% (0)
89.5% (179)
9.5% (19)
1% (2)
Sert and Bayirli [37], 2004

Subgroup Ethnic Incidence

1200
96%  (979)
6.67%  (21)

67%  (795)
31%  (372)
1.46%  (21)
0.63%  (7)
69.85%  (680)
6.75%  (68)
4.73%  (41)
6.53%  (94)
9.85%  (125)
0.5%  (6)
0.4%  (6)
0.25%  (2)
0.95%  (7)
79%  (595)
20%  (100)
0.44%  (5)

Hispanic

Mexico
In vitro: radiograph
202

79.3% (140)
25.8% (62)
0% (0)
0% (0)

74.2% (150)
25.8 (52)
0% (0)
Pineda and Kuttler [38], 1972

Total no of teeth

12752
7395
200
12
6070
2027
73
181
2868
137
100
394
382
8
7
10
9
4349
1002
13

Overall Incidence (%]

97.21  (%)
2.63  (%)
0.16  (%)
73.55  (%)
23.55  (%)
0.91  (%)
2  (%)
72.6  (%)
3.73  (%)
2.72  (%)
10.72  (%)
10.4  (%)
0.22  (%)
0.19  (%)
0.27  (%)
0.24  (%)
81.08  (%)
18.68  (%)
0.24  (%)

Table 2: Summary of the incidences of documented ethnic and population variations in the anatomy of mandibular second premolars.

Geographic and ethnicdistribution
Materials and methods
No. of teeth in the study ()
Root anatomy
Root canal anatomy
Root canal pattern (Vertucci)
Apical foramina
References

1 root
2 roots
>2 roots
1 canal
2 canals
>2 canals
C-shaped
Type I
Type II
Type III
Type IV
Type V
Type VI
Type VII
Type VIII
Others types
1
2
3

Caucasian

France
In vitro: radiograph and sectioning
328
97.6% (320)
2.4% (8)
0%(0)
86.6% (284)
13.4% (44)

Geider et al. [4], 1989

Germany
In vitro: analysis of extracted teeth
2089
99.85% (2086)
0.05% (1)
0.1% (2)

Visser [41], 1948

Poland
In vitro: extraction and RCT
56

68.2% (38)
31.8% (18)
0%(0)
0%(0)

Rózyło et al. [6], 2008

U.S.A
In vitro: clearing
400
100% (400)
0%(0)
0%(0)
97.5% (390)
2.5% (10)
0%(0)
0%(0)
97.5% (390)
0%(0)
0%(0)
0%(0)
2.5% (10)
0%(0)
0%(0)
0%(0)
0%(0)
97.5% (390)
2.5% (10)
0%(0)
Vertucci [7], 1984

In vitro: sectioning
32
100% (32)
0%(0)
0%(0)
65.6% (21)
34.4% (11)

Barrett [11], 1925

In vitro: extraction and radiograph
906
96.6% (902)
0%(0)
3.4% (4)
87.5% (792)
12.5  (112)
0.4% (2)
0%(0)

Zillich and Dowson [12], 1973

In vitro: grinding and examination under magnification
50

92% (46)
8% (4)
0%(0)
0%(0)

96% (48)
4% (2)
0%(0)
Green [9], 1973

Subgroup ethnic incidence

3861
98.81%
0.49%
0.7%
82.9%
17.1%
0.07%
0%
97.5%

2.5%

96.75%
3.25%

African

Senegalese
In vivo: radiographic examination
408

86% (351)
12% (49)
2% (8)
0%(0)

Mbaye et al. [15], 2008

Indian

India
In vitro: clearing
40

80% (32)
20%(8)
0%(0)
0%(0)
80% (32)
0%(0)
0%(0)
2.5% (1)
17.5% (7)
0%(0)
0%(0)
0%(0)
0%(0)
80% (32)
20% (8)
0%(0)
Parekh et al. [19], 2011

In vitro: SCT
96
97.9% (94)
2.1% (2)
0%(0)
87% (83)
13.5% (13)
0%(0)
0%(0)
80% (78)
9% (9)
3% (3)
2% (2)
4% (4)
0%(0)
0%(0)
0%(0)
0%(0)
94% (90)
6% (6)
0%(0)
V. K. Sikri and P. Sikri [21], 1994

Subgroup ethnic incidence

136
97.9%
2.1%
0%
83.5%
16.75%
0%
0%
80%
4.5%
1.5%
2.25%
10.75%

87%
13%

Mongoloid

China
In vivo: CBCT
178
100% (178)
0%(0)
0%(0)
97.2% (173)
2.2%(4)
0%(0)
0.6% (1)
97.2% (173)
0%(0)
0%(0)
0.5% (1)
1.69% (3)
0%(0)
0%(0)
0%(0)
0.5% (1)

Tian et al. [25], 2012

Japan
In vitro: radiograph
40

97.9% (39)
2.1%(1)
0%(0)
0%(0)

Miyoshi et al. [29], 1977

Korea
CBCT
789
99.4% (784)
0.6% (5)
0%(0)

Park et al. [31], 2013

Subgroup ethnic incidence

1007
99.7%
0.3%
0%
97.5%
2.15%
0%
0.3%
97.2%

0.5%
1.69%

0.5%

Middle east

Kuwait
In vitro: radiograph of RCT teeth
64
95.6% (61)
4.4% (3)
0%(0)
95.3% (61)
4.7%(3)
0%(0)
0%(0)

95.3% (61)
4.7% (3)
0%(0)
Zaatar et al. [32], 1997

Jordan
In vitro: clearing
400
97.2% (389)
2.8% (11)
0%(0)
72% (288)
27.5% (110)
0.5% (2)
0%(0)
78% (288)
3.8% (15)
0.1% (4)
7.5% (30)
15.3% (61)
0%(0)
0%(0)
0%(0)
0.5% (2)
74.2% (337)
15.8% (63)
0%(0)
Awawdeh and Al-Qudah [33], 2008

Iran
In vitro: clearing
103
100% (103)
0%(0)
0%(0)
80.5% (83)
17.5% (18)
0%(0)
2% (2)
76.3% (76)
7.9% (9)
9.9% (11)
5.9% (7)
0%(0)
0%(0)
0%(0)
0%(0)
0%(0)
94.1% (97)
5.9% (6)
0%(0)
Rahimi et al. [35], 2007

In vitro: clearing and sectioning
80

88.8% (71)
11.2% (9)
0%(0)
0%(0)
91.2% (73)
0%(0)
0%(0)
0%(0)
0%(0)
0%(0)
0%(0)
0%(0)
0%(0)
97.5% (78)
2.5% (2)
0%(0)
Hasheminia and Hashemi [39], 2005

In vitro: clearing
137

94.2% (129)
5.8%(8)
0%(0)
0%(0)

75.9% (104)
24.1% (33)
0%(0)
Rahimi et al. [40], 2009

Turkey
In vitro: clearing
100
100% (100)
0%(0)
0%(0)
93.6% (94)
6.4%(6)
0%(0)
0%(0)
93.6% (94)
0%(0)
0%(0)
0%(0)
6.4% (6)
0%(0)
0%(0)
0%(0)
0%(0)
93.6% (94)
6.4% (6)
0%(0)
Çalişkan et al. [36], 1995

In vitro: clearing
200
100% (200)
0%(0)
0%(0)
71% (142)
29% (58)
0%(0)
0%(0)
71% (142)
7% (14)
3.5% (7)
9% (18)
7% (14)
1.5% (3)
1% (2)
0%(0)
0%(0)
81.5% (163)
18.5% (37)
0%(0)
Sert and Bayirli [37], 2004

Subgroup ethnic incidence

1084
98.56%
1.44%
0%
85.06%
14.59%
0.07%
0.29%
82.02%
3.74%
2.7%
4.48%
5.74%
0.3%
0.2%

0.1%
87.44%
11.13%

Hispanic

Mexico
In vitro: radiographs
250

98.8% (247)
1.2%(3)
0%(0)
0%(0)

98.8% (247)
1.2% (3)
0%(0)
Pineda and Kuttler [38], 1972

Total no of teeth

6746
5649
34
6
3364
489
12
3
1346
46
46
58
105
3
2
0
3
1741
179
0

Overall incidence

99.28%
0.61
0.01%
86.9%
12.64%
0.31%
0.08%
83.65%
2.86%
2.86%
3.6%
6.52%
0.18%
0.12%
0%
0.18%
90.68%
9.32%
0%

3.1. Radicular Anatomy with Ethnic and Demographic Patterns

In most instances, mandibular first premolars were found to have one root (97.21%). However, only 73.55% of these single rooted teeth contained a single canal. Thus, although the incidence of two roots was low (2.63%), about 23.55% teeth had two canals. Higher incidences of two roots were noted in the African-American population (16.2%) [14] and a Kuwaiti population (15%) [32]. Three or more rooted forms were reported in only 3% French [4] and 0.2% Indian [17] populations, respectively.

A higher incidence of two canals in mandibular first premolars was reported in several populations, upto 50% in Indian populations [16, 18–21] and approximately 40% in Middle Eastern populations from Kuwait [32], Jordan [33], and Turkey [36, 37]. While the Hispanic population [38] in Mexico also showed an incidence of 30.7%, the Chinese [22–27] and Caucasian [4, 6–14] populations had a variable incidence of two canals in about 10.7–36%. The incidence of three or more canals in mandibular first premolars was considerably lower (0–5%) [7, 12, 15, 22, 25, 27, 30, 33, 35, 36], with no such canal systems noted in Indian and Hispanic populations.

On the other hand, mandibular second bicuspids presented with a higher incidence of one root (99.28%) and one canal (86.9%). Overall, a second root was present in about 0.0–4.4% (average 0.61%) of teeth. In different studies, Caucasians [4, 6, 7, 9, 11, 12] and Turkish [36, 37] populations presented with a varied incidence of a second canal ranging from 2.5 to 34.4% and 6.4 to 29%, respectively. Second premolars presented with a second canal in Indian (13.5–20%) [19, 21], Iranian (5.8–17.5%) [35, 39, 40], or Jordanian (22.8%) [33] populations. However, Mongoloid [25, 29] and Hispanic [38] populations presented a much lower incidence of a second canal, 2% and 1.2%, respectively. Three or more canals in mandibular second premolars were scarcely reported (0–2%) [12, 15, 33].

C-shaped canal anatomy has been mainly documented in mandibular first bicuspids. Studies have reported a high incidence of C-shaped canals in Chinese populations ranging from 18 to 24% [23, 28], while other Chinese studies [25–27] have reported a lower incidence of 0–4–1.1%. In an Indian population, V. K. Sikri and P. Sikri [21] reported 10% first premolars exhibiting C-shaped canals while Sandhya et al. [16] reported the variation in 2% teeth in a southern Indian population. C-shaped canals were found to be of low incidence in second bicuspids, with only 0.6% and 2% incidence reported, respectively, in a Chinese [25] and an Iranian [35] study.

Lu et al. [23] coined the term “circumferential canals,” for an unusual aberration found in 6% of mandibular first premolars. A circumferential canal was described as a single canal in the center with 3 or 4 canals at the circumference when viewed in cross-section, that is, a single canal splitting into several canals (apical delta) at apical 3 mm from sagital view. The teeth had a single oval canal or two canals in an oval-shaped root while the C-shaped morphology was found in the apical 3 mm and/or 6 mm level cross-sections.

In both premolars, single canals were most likely to be an independent canal from orifice to apical foramen (Type I). However, in first premolar, a significant number of bifid canals fused prior to exit (Type II) in Indian and Turkish populations (9–18%) [16–21, 37]. Type IV pattern was more prevalent than Type V pattern in most population groups. However, Type V pattern (9–22%) was much higher than Type IV patterns (0–10%) in Mongoloids pointing to a higher prevalence of a singular canal bifurcating along its length, somewhat similar to the description of the above-mentioned circumferential canals. In second premolars, the Type V pattern was more prevalent than Type IV in all population groups, with significantly higher prevalence of upto 15–17% in Indian and Jordanian. A single apical foramen was present in 81% first premolars and 90% second premolars. The mandibular first premolar was more prone to bifurcation of canals (23–30%) terminating in multiple apical foramina (15–20%).

Based on the statistical analysis performed, a significant variation in the number of roots, root canals, and apical foramen was observed between all the ethnicities, in both mandibular bicuspids. Thus, based on this analysis, the number of roots, root canals, and apical foramen were significantly different in Caucasian, Indian, Mongoloid, and Middle Eastern populations. Additionally, there was also a significant difference in the root canal configurations of these population groups. However, in case of comparing for anatomic variations within populations of the same ethnicity, statistics could be performed only when sufficient data allowed for statistical analysis. The numbers of roots and root canals in first and second premolars were statistically significantly different in French, German, Polish, and the United States populations of Caucasians, as well as among the first premolars of Middle Eastern populations, Turkey, Jordan, Kuwait, and Iran. However, no such statistical significance was observed between Chinese and Korean second premolars.

Six ex vivo studies have reported the presence of a deep external mesial invagination (mesial invagination) along the root surface of mandibular premolars [16, 18, 20, 24, 33, 42] (Table 3). Sandhya et al. [16] assessed the root dentin thickness at the depth of the mesial invagination in mandibular first premolars and reported the average root thickness at the cervical, middle, and apical thirds to be 0.8, 0.78, and 0.3 mm, respectively. All C-shaped configuration premolars could also present an associated groove or concavity on the proximal lingual area of the middle root that would not always extend to the root apex. Some grooves presented as deep, folding grooves while others were not so distinguished or were just shallow concavities [23].

Table 3: Summary of exvivo studies reporting a deep mesial radicular invagination in mandibular premolars.

Population
Incidence
Tooth type
References

India
14%
1st premolar
Velmurugan and Sandhya [18], 2009

14%
1st premolar
Sandhya et al. [16], 2010

15.21%
1st premolar
Jain and Bahuguna [20], 2011

China
27.8%
1st premolar
Liu et al. [24], 2013

Jordan
17.6%
1st premolar
Awawdeh and Al-Qudah [33], 2008

13.5%
2nd premolar

Austria
15%
1st premolar
Robinson et al. [42], 2002

3.2. Gender Predilection

There is little documentation correlating the influence of gender on root/canal anatomy and its variations. Of the reported studies, females had a higher likelihood of two or more roots or canals in mandibular first premolars, whereas men exhibited multiple canals much more frequently than females in mandibular second premolars [17, 37, 43]. Others have reported no significant difference in root configuration between females and males [29].

3.3. Case Reports

In addition to these numerous population studies, thirty-six case reports have also documented anatomic variations. The anatomic aberrations reported as endodontic case reports in the literature include mandibular first premolars with 2 or 3 canals in 1 root or 2 roots; 3 roots and 3 canals; and 4 canals in 4 roots (Table 4) [44–59]. Mandibular second premolars have shown variations that include 2, 3, 4, and 5 canals in 1 root; 2, 3, and 4 canals in 2 roots; 3 roots and 3 or 4 canals; (Table 5) [46, 49, 53–55, 60–80].

Table 4: Table enlisting documented case reports of mandibular first premolars with multiplicity of roots or root canals in teeth presenting.

Number of roots
Root canal anatomy
Diagnostic method
Country
Reference

1 root
2 canals
R/G
USA
England et al. [44], 1991

2 canals
R/G
India
Shenoy et al. [45], 2013

3 canals
R/G
Jamaica
Nallapati [46], 2005

3 canals
R/G
Brazil
De Almeida-Gomes et al. [47], 2006

2 roots
2 canals
R/G
India
Kararia et al. [48], 2012

3 canals
R/G
India
Poorni et al. [49] 2010

3 canals
R/G
India
Moayedi and Lata [50], 2004

N/A
R/G
USA
Milano et al. [51], 2002

3 roots
3 canals
R/G
India
Kakkar and Singh [52], 2012

3 canals
R/G
China
Chan et al. [53], 1992

3 canals
Extraction
USA
Fischer and Evans [54], 1992

3 canals
Micro CT after extraction
Britain
Cleghorn et al. [55], 2008

N/A
3 canals
R/G
Germany
Hlsmann [56], 1990

N/A
3 canals
R/G
Australia
Yang [57], 1994

4 roots
4 canals
R/G
India
Vaghela and Sinha [58], 2013

—
4 canals
R/G
China
Du et al. [59], 2013

N/A: not available, R/G: radiograph, and micro-CT: microcomputed tomography.

Table 5: Table summarizing case reports of anatomical variations of roots and root canals in mandibular second premolars.

Number of roots
Root canal anatomy
Diagnostic mode
Country
Reference

1 root
3 canals
R/G
USA
Nallapati [46], 2005

3 canals
R/G
India
N. Kararia and V. Kararia [60], 2013

4 canals
R/G
Isreal
Holtzman [61], 1998

4 canals
R/G
USA
Wong [62], 1991

5 canals
R/G
Argentina
Macri and Zmener [63], 2000

C-shaped
Micro-CT after extraction
Britain
Cleghorn et al. [55], 2008

2 roots
2 canals
R/G
India
Goswami et al. [64], 1997

2 canals
R/G
India
Prakash et al. [65], 2008

3 canals
R/G
China
Chan et al. [53], 1992

3 canals
R/G
Germany
Rödig and Hülsmann [66], 2003

3 canals
R/G
Belgium
De Moor and Calberson. [67], 2005

3 canals
R/G
Iran
Lotfi et al. [68], 2008

3 canals
R/G
Brazil
Soares et al. [69], 2009

3 canals
R/G
Iran
Shokouhinejad [70], 2009

3 canals
R/G
India
Aguiar et al. [71], 2010

3 canals
R/G
India
Poorni et al. [49], 2010

4 canals
R/G
USA
Bram and Fleisher [72], 1991

4 canals
R/G
Saudi Arabia
Al-Fouzan [73], 2001

4 canals
R/G
United Kingdom
Rhodes [74], 2001

4 canals
R/G
Greece
Tzanetakis et al. [75], 2007

3 canals
Extraction
USA
Fischer and Evans [54], 1992

3 canals
R/G
Iran
Shalavi et al. [76], 2012

3 canals
CBCT
Iran
Mokhtari et al. [77], 2013

4 canals
Extraction
USA
Shapira and Delivanis [78], 1982

4 roots
4 canals
SCT
Indian
Sachdeva et al. [79], 2008

4 canals
R/G
Greece
Farmakis [80], 2008

CBCT: cone beam computed tomography, Micro-CT: microcomputed tomography, SCT: spiral computed tomography, and R/G: radiograph.

3.4. Anatomic Developmental Anomalies

Adding to the complexity of the mandibular premolars are various developmental anomalies (Table 6) [77, 81–90]. In 1997, Hartup [81] reported a Type III dens invaginatus and a bifurcated root of the mandibular first premolar. Tavano et al. [83] reported a dens invaginatus wherein the clinical crown was larger than the contralateral first premolar. No case reports of dens invaginatus in the mandibular second premolar have been reported (Table 6). Conversely, dens evaginatus most frequently affected mandibular second premolars and was more often reported in Mongoloid people [88].

Table 6: Summary of various developmental anomalies that have been reported in mandibular premolars.

Mandibular premolar
Developmental anomalies
Root/root canal anatomy
Diagnostic mode
Reference

1st premolar
Dens invaginatus
2 roots
R/G
Hartup [81], 1997

Dens invaginatus
N/A
R/G
Bramante et al. [82], 1993

Dens invaginatus
N/A
R/G
Tavano et al. [83], 1994

Gemination
3 canals
R/G
Aryanpour et al. [84], 2002

Dens invaginatus
1 root
R/G
Er et al. [85], 2007

Dens invaginatus (bilateral)
N/A
R/G
Canger et al. [86], 2009

2nd premolar
Dens evaginatus
N/A
R/G
Koh et al. [87], 2001

Fusion with mandibular first molar
5 canals
R/G
Tsesis et al. [88], 2003

Fusion with supernumerary tooth
N/A
Clinical
Sathish Muthukumar et al. [89], 2012

Taurodontism
3 canal
CBCT
Mokhtari et al. [77], 2013

Taurodontism
5 canals
R/G
Demiryürek et al. [90], 2013

CBCT: cone beam computed tomography, R/G: radiograph, and N/A: not available.

Aryanpour et al. [84] reported root canal and periodontal treatment of a geminated mandibular first premolar, that is, two distinct crowns with united roots, with three canals. Kusaik et al. [91] reported the root morphology of mandibular premolars in female Polish patients with Turners syndrome, based on orthopantomogram X-ray images. They reported two-rooted mandibular teeth in 31–34% first premolars and 31–39% second premolars, which is much higher than that reported in general populations (<5%).

4. Discussion

Several factors contribute to variations found in the root and root canals that include ethnicity and gender. Scott and Turner [92], in their anthropological review, described the accessory root of first mandibular premolars as Tome’s root, which showed a high incidence of greater than 25% in ethnic Australians and sub-Sahara African populations. Contrastingly, American Arctic, New Guinea, Jomon, and Western Eurasian populations had a lower incidence of the accessory root (0 to 10%). Such anthropological data could provide valuable information regarding the likelihood of an additional root in mandibular premolars of specific ethnic populations. Thus, the role of genetics should also not be underestimated in determining anatomic variations of human teeth.

Numerous methods have been used for studying root canal anatomy, including replication techniques [93], ground sections [94], clearing techniques [95], and radiography [96]. Advanced modes of radiographic imaging and analysis have allowed for in-depth knowledge of pulp space anatomy in three dimensions and allowed for identification of rare aberrations. These methods include spiral computed tomography (SCT) [16, 21], micro-computed tomography (micro CT) [24, 28, 97], and cone beam computed tomography (CBCT) [25–27]. Study design differences and the various origins of the investigated teeth could account for the highly variable results.

Ex vivo anatomic investigations, although the most widely used, inherently have certain shortcomings. It involves evaluation of extracted teeth that are frequently difficult to collect in sufficient numbers along with known specifics like age, gender, and so forth. Furthermore, most extracted teeth collected are severely damaged leading to difficulties in determining accurately the tooth notation. An additional negative impact if only sound teeth are selected is selection bias [25, 30]. Additionally, not all anatomic studies assessing mandibular premolars have reported the root and root canal configuration. Either one of these has been described with most studies discussing the root canal configuration (Tables 1 and 2). It would be of much more analytic and scientific value if the maximum data could be presented from the analyzed teeth, especially when the methodology leads to destruction of the samples. Thus, although a large number of mandibular premolars have been analyzed in anatomic studies, maximum extrapolation is lacking. CBCT imaging by means of its minimal radiation exposure, sample preservation, accuracy, and three-dimensional data acquisition of multiple teeth, could overcome the shortfalls of ex vivo methods and offer maximum information without solely depending on analysis of extracted teeth.

Differences in the method of analysis and data presentation could also contribute to an inaccurate perception of the incidence of variable anatomy. Data presented by number of patients instead of by number of teeth generally results in higher frequencies of the reported anomaly [14]. Also, the sample size studied in relation to the total sample of the population plays an important role in the overall ratio of variations. For instance, the only study analyzing the anatomy of a Kuwaiti population assessed only 20 mandibular first premolars of which 15% were two-rooted teeth and 40% contained two canals. Although these values are above the general weighted average or incidence among neighboring population groups, these findings may not reliably signify the accurate incidence of tooth anatomy within the population. Thus, studies of larger sample groups and streamlining data in the form of number of teeth could enhance comparison and also give a more dependable picture of the prevalent anatomy.

An interesting pattern that can be observed is that case reports of the second premolar by far outnumber those of the first premolar, especially in reported variations. This is contrary to the findings of epidemiologic studies, which reported an increased possibility of anatomic variation in mandibular first premolars. Despite these contrasting findings, anatomic studies serve as an indicator of the possible anatomic variations in analyzed population groups and should not be considered as the lone guiding principle. Wider anatomic variations are certainly possible in any tooth, as also in teeth that are less likely to show aberrations. On the other hand, case reports could be misleading to the clinician with regard to the incidence of the documented aberrations; however, their didactic value is of extreme importance. It allows the clinician to be in the best position to detect, discern, and diagnose various previously documented in vivo anatomic variations.

Pucci and Reig [98], in their monumental work Conductos Radiculares, concluded that the mandibular second premolar had two canals and two foramina 11.5% of the time, whereas the mandibular first premolar had branching canals, apical bifurcations, and trifurcations 26.5% of the time. Cleghorn et al. [99, 100], in a review of mandibular first and second premolars, presented findings regarding the number of roots, number of canals, and apical morphology. They reported that approximately 98% of mandibular first premolars were single-rooted with a single canal in 75.8% of the teeth. In cases of second premolars, almost all of the teeth in the anatomic studies were single-rooted (99.6%) with a single canal (91.0%). Comparatively, the findings of the present systematic review are more or less in agreement with these observations of Pucci and Reig [98] and Cleghorn et al. [99, 100].

The data analyzed in this systematic review is secondary data that is sourced from numerous previously published studies. Such secondary data is prone to drawbacks or biases that are inherent in the original data which could ultimately reflect in the results of this study as well. For instance, the method of analysis or the minimal number of teeth analyzed in a particular study. However, the intention was to provide the dental and endodontic fraternity, an interpretation of the vast data on mandibular premolars with a possibility of correlation to geographical origin and ethnicity that was not previously available.

The descriptions of the frequently occurring root and canal forms of permanent teeth are based largely on studies conducted in Europe and North America and relate to teeth of predominantly Caucasoid origin [1, 3]. The descriptions may not be wholly applicable to teeth of non-Caucasoid origin. The present systemic review provides additional and up-to-date information regarding the canal configuration of premolars and their apical exit patterns which allows for further comparison among different population groups around the globe. However, a very slight trend of more varied anatomy, that is, two or more roots or canals, could be seen in recent anatomic studies using modern imaging techniques, in as yet lesser analyzed population groups. Future documentation of root and canal anatomy in previously lesser studied geographical populations could show a trend of discrepancies between previously established weighted averages. However, it would be more appropriate and accurate to base successive comparisons of anatomic averages on ethnicities and demography’s, as against the general norm of overall weighted averages.

5. Conclusions

(1)The mandibular first premolar was more prone to bifurcation of canals (23–30%) terminating in multiple apical foramina (15–20%), as compared to second premolars.(2)The C-shaped canal pattern was most prevalent in first premolars of Chinese populations (upto 24%).(3)In second premolars, Caucasian, Indian, and Middle Eastern populations showed a higher prevalence of multiple canals (14–17%).(4)Type I canal configuration was most prevalent in both first (72.6%) and second premolars (83.65%).(5)A deep mesial radicular invagination was a common finding in multiple population groups in first premolars (13–27%).(6)Dens invaginatus was the most common developmental anomaly in first premolars.(7)There exists an association between ethnicity and root, root canal morphology across population groups in first and second premolars.

Recent imaging techniques and evaluation of wider populations have given a better insight regarding mandibular premolar anatomy and their inherent variations. Certain population and geographic groups have little or no data regarding mandibular premolar morphology, especially in South American, African, Australasian, and South East Asian populations. Although the studies that have been covered under this review did provide relevant information regarding root and root canal anatomy, other important data relating to apical anatomy, relationship of the anatomic apex with the apical foramen, precise location of bifurcations when present, mesiodistal width of roots, areas along the root that show narrowing or thinning, canal isthmuses, canal curvature, and so forth needs further evaluation and documentation in future research. Racial differences and its influence on pulp space anatomy should always be kept in mind.

Conflict of Interests

The authors declare that there is no conflict of interests regarding the publication of this paper.

Acknowledgments

The authors thank Dr. Basil Joy (Postgraduate Student, Department of Conservative Dentistry and Endodontics) and Dr. Asha K Bava (Intern), Mar Baselios Dental College, Kerala, India, for the help received in preparing this paper.

References

R. D'Souza, “Development of the pulpodentinal complex,” in Seltzer and Bender's Dental Pulp, K. Hargreaves, Ed., Quintessence Books, Chicago, Ill, USA, 2002.

F. J. Vertucci, “Root canal morphology and its relationship to endodontic procedures,” Endodontic Topics, vol. 10, pp. 3–29, 2005.

P. M. Butler, “Dental merism and tooth development,” Journal of Dental Research, vol. 46, no. 5, pp. 845–850, 1967.

P. Geider, C. Perrin, and M. Fontaine, “Endodontic anatomy of lower premolars–apropos of 669 cases,” Journal d'Odontologie Conservatrice, no. 10, pp. 11–15, 1989 (French).

C. Schulze, “Developmental abnormalities of teeth and jaws,” in Thoma's Oral Pathology, R. Gorlin and H. Goldman, Eds., pp. 106–107, CV Mosby, St. Louis, Mo, USA, 6th edition, 1970.

T. K. Rózyło, M. Miazek, I. Rózyło-Kalinowska, and F. Burdan, “Morphology of root canals in adult premolar teeth,” Folia Morphologica, vol. 67, no. 4, pp. 280–285, 2008.

F. J. Vertucci, “Root canal anatomy of the human permanent teeth,” Oral Surgery Oral Medicine and Oral Pathology, vol. 58, no. 5, pp. 589–599, 1984.

C. L. Sabala, F. W. Benenati, and B. R. Neas, “Bilateral root or root canal aberrations in a dental school patient population,” Journal of Endodontics, vol. 20, no. 1, pp. 38–42, 1994.

D. Green, “Double canals in single roots,” Oral Surgery Oral Medicine and Oral Pathology, vol. 35, no. 5, pp. 689–696, 1973.

M. K. Baisden, J. C. Kulild, and R. N. Weller, “Root canal configuration of the mandibular first premolar,” Journal of Endodontics, vol. 18, no. 10, pp. 505–508, 1992.

M. Barrett, “The internal anatomy of the teeth with special reference to the pulp and its branches,” Dental Cosmos, vol. 67, pp. 581–592, 1925.

R. Zillich and J. Dowson, “Root canal morphology of mandibular first and second premolars,” Oral Surgery Oral Medicine and Oral Pathology, vol. 36, no. 5, pp. 738–744, 1973.

A. Mueller, “Anatomy of the root canals of the incisors, cuspids and bicuspids of the permanent teeth,” The Journal of the American Dental Association, vol. 20, pp. 1361–1386, 1933.

M. Trope, L. Elfenbein, and L. Tronstad, “Mandibular premolars with more than one root canal in different race groups,” Journal of Endodontics, vol. 12, no. 8, pp. 343–345, 1986.

M. Mbaye, B. Touré, A. W. Kane, F. Leye, K. Bane, and Y. Boucher, “Radiographic study of the canal anatomy of mandibular premolars in a Senegalese population,” Dakar Médical, vol. 53, no. 3, pp. 267–271, 2008.

R. Sandhya, N. Velmurugan, and D. Kandaswamy, “Assessment of root canal morphology of mandibular first premolars in the Indian population using spiral computed tomography: an in vitro study,” Indian Journal of Dental Research, vol. 21, no. 2, pp. 169–173, 2010.

V. Iyer, R. Indira, S. Ramachandran, and M. Srinivasan, “Anatomical variations of mandibular premolars in Chennai population,” Indian Journal of Dental Research, vol. 17, no. 1, pp. 7–10, 2006.

N. Velmurugan and R. Sandhya, “Root canal morphology of mandibular first premolars in an Indian population: a laboratory study,” International Endodontic Journal, vol. 42, no. 1, pp. 54–58, 2009.

V. Parekh, N. Shah, and H. Joshi, “Root canal morphology and variations of mandibular premolars by clearing technique: an in vitro study,” Journal of Contemporary Dental Practice, vol. 12, no. 4, pp. 318–321, 2011.

A. Jain and R. Bahuguna, “Root canal morphology of mandibular first premolar in a Gujarati population-an in vitro study,” Dental Research Journal, vol. 8, pp. 118–122, 2011.

V. K. Sikri and P. Sikri, “Mandibular premolars: aberrations in pulp space morphology,” Indian Journal of Dental Research, vol. 5, no. 1, pp. 9–14, 1994.

R. T. Walker, “Root canal anatomy of mandibular first premolars in a southern Chinese population,” Endodontics & dental traumatology, vol. 4, no. 5, pp. 226–228, 1988.

T.-Y. Lu, S.-F. Yang, and S.-F. Pai, “Complicated root canal morphology of mandibular first premolar in a Chinese population using the cross section method,” Journal of Endodontics, vol. 32, no. 10, pp. 932–936, 2006.

N. Liu, X. Li, N. Liu et al., “A micro-computed tomography study of the root canal morphology of the mandibular first premolar in a population from southwestern China,” Clinical Oral Investigations, vol. 17, pp. 999–1007, 2013.

Y.-Y. Tian, B. Guo, R. Zhang et al., “Root and canal morphology of maxillary first premolars in a Chinese subpopulation evaluated using cone-beam computed tomography,” International Endodontic Journal, vol. 45, no. 11, pp. 996–1003, 2012.

Q. Liao, J. L. Han, and X. Xu, “Analysis of canal morphology of mandibular first premolar,” Shanghai Kou Qiang Yi Xue, vol. 20, pp. 517–521, 2011 (Chinese).

Y.-Y. Tian, B. Guo, R. Zhang et al., “Root and canal morphology of maxillary first premolars in a Chinese subpopulation evaluated using cone-beam computed tomography,” International Endodontic Journal, vol. 39, pp. 435–438, 2013.

B. Fan, J. Yang, J. L. Gutmann, and M. Fan, “Root canal systems in mandibular first premolars with C-shaped root configurations. Part I: microcomputed tomography mapping of the radicular groove and associated root canal cross-sections,” Journal of Endodontics, vol. 34, no. 11, pp. 1337–1341, 2008.

S. Miyoshi, J. Fujiwara, T. Tsuji YNakata, and K. Yamamoto, “Bifurcated root canals and crown diameter,” Journal of Dental Research, vol. 56, no. 11, p. 1425, 1977.

T. Yoshioka, J. C. Villegas, C. Kobayashi, and H. Suda, “Radiographic evaluation of root canal multiplicity in mandibular first premolars,” Journal of Endodontics, vol. 30, no. 2, pp. 73–74, 2004.

J. B. Park, N. Kim, S. Park, Y. Kim, and Y. Ko, “Evaluation of root anatomy of permanent mandibular premolars and molars in a Korean population with cone-beam computed tomography,” European Journal of Dentistry, vol. 7, pp. 94–101, 2013.

E. I. Zaatar, A. M. Al-Kandari, S. Alhomaidah, and I. M. Al-Yasin, “Frequency of endodontic treatment in Kuwait: radiographic evaluation of 846 endodontically treated teeth,” Journal of Endodontics, vol. 23, no. 7, pp. 453–456, 1997.

L. A. Awawdeh and A. A. Al-Qudah, “Root form and canal morphology of mandibular premolars in a Jordanian population,” International Endodontic Journal, vol. 41, no. 3, pp. 240–248, 2008.

S. Khedmat, H. Assadian, and A. A. Saravani, “Root canal morphology of the mandibular first premolars in an Iranian population using cross-sections and radiography,” Journal of Endodontics, vol. 36, no. 2, pp. 214–217, 2010.

S. Rahimi, S. Shahi, H. R. Yavari, H. Manafi, and N. Eskandarzadeh, “Root canal configuration of mandibular first and second premolars in an Iranian population,” Journal of Dental Research, Dental Clinics, Dental Prospects, vol. 1, pp. 59–64, 2007.

M. K. Çalişkan, Y. Pehlivan, F. Sepetçioǧlu, M. Türkün, and S. Ş. Tuncer, “Root canal morphology of human permanent teeth in a Turkish population,” Journal of Endodontics, vol. 21, no. 4, pp. 200–204, 1995.

S. Sert and G. S. Bayirli, “Evaluation of the root canal configurations of the mandibular and maxillary permanent teeth by gender in the Turkish population,” Journal of Endodontics, vol. 30, no. 6, pp. 391–398, 2004.

F. Pineda and Y. Kuttler, “Mesiodistal and buccolingual roentgenographic investigation of 7,275 root canals,” Oral Surgery, Oral Medicine, Oral Pathology, vol. 33, no. 1, pp. 101–110, 1972.

M. Hasheminia and A. Hashemi, “Frequency of canal configuration in maxillary first premolar and mandibular second premolars,” Journal of Isfahan Dental School, vol. 1, pp. 59–64, 2005.

S. Rahimi, S. Shahi, H. R. Yavari, M. F. Reyhani, M. E. Ebrahimi, and E. Rajabi, “A stereomicroscopy study of root apices of human maxillary central incisors and mandibular second premolars in an Iranian population,” Journal of Oral Science, vol. 51, no. 3, pp. 411–415, 2009.

J. Visser, Beitrag zur Kenntnis der menschlichen Zahnwurzelformen [medical dissertation], Universitat Zurich, Zurich, Switzerland, 1948.

S. Robinson, C. Czerny, A. Gahleitner, T. Bernhart, and F. M. Kainberger, “Dental CT evaluation of mandibular first premolar root configurations and canal variations,” Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics, vol. 93, no. 3, pp. 328–332, 2002.

N. J. Serman and G. Hasselgren, “The radiographic incidence of multiple roots and canals in human mandibular premolars,” International Endodontic Journal, vol. 25, no. 5, pp. 234–237, 1992.

M. C. England Jr., G. R. Hartwell, and J. R. Lance, “Detection and treatment of multiple canals in mandibular premolars,” Journal of Endodontics, vol. 17, no. 4, pp. 174–178, 1991.

A. Shenoy, N. Bolla, S. Vemuri, and J. Kurian, “Endodontic retreatment-unusual anatomy of a maxillary second and mandibular first premolar: report of two cases,” Indian Journal of Dental Research, vol. 24, pp. 123–127, 2013.

S. Nallapati, “Three canal mandibular first and second premolars: a treatment approach. A case report,” Journal of Endodontics, vol. 31, no. 6, pp. 474–476, 2005.

F. de Almeida-Gomes, B. C. de Sousa, and R. A. dos Santos, “Unusual anatomy of mandibular premolars,” Australian Endodontic Journal, vol. 32, no. 1, pp. 43–45, 2006.

N. Kararia, A. Chaudhary, and V. Kararia, “Mandibular left first premolar with two roots: a morphological oddity,” Contemporary Clinical Dentistry, vol. 3, pp. 234–236, 2012.

S. Poorni, C. S. Karumaran, and R. Indira, “Mandibular rst premolar with two roots and three canals,” Australian Endodontic Journal, vol. 36, no. 1, pp. 32–34, 2010.

S. Moayedi and D. Lata, “Mandibular first premolar with three canals,” Endodontology, vol. 16, pp. 26–29, 2004.

M. Milano, C. Chavarria, and J. Hoppe, “Multi-rooted mandibular premolars: report of case,” Journal of Dentistry for Children, vol. 69, no. 1, pp. 63–65, 2002.

P. Kakkar and A. Singh, “Mandibular first premolar with three roots: a case report,” Iranian Endodontic Journal, vol. 7, pp. 207–210, 2012.

K. Chan, S. C. Yew, and S. Y. Chao, “Mandibular premolar with three root canals—two case reports,” International Endodontic Journal, vol. 25, no. 5, pp. 261–264, 1992.

G. M. Fischer and C. E. Evans, “A three-rooted mandibular second premolar,” General Dentistry, vol. 40, no. 2, pp. 139–140, 1992.

B. M. Cleghorn, W. H. Christie, and C. C. S. Dong, “Anomalous mandibular premolars: a mandibular first premolar with three roots and a mandibular second premolar with a C-shaped canal system,” International Endodontic Journal, vol. 41, no. 11, pp. 1005–1014, 2008.

M. Hülsmann, “Mandibular first premolar with three root canals,” Endodontics & Dental Traumatology, vol. 6, no. 4, pp. 189–191, 1990.

Z. P. Yang, “Multiple canals in a mandibular first premolar. Case report,” Australian Dental Journal, vol. 39, no. 1, pp. 18–19, 1994.

D. J. Vaghela and A. A. Sinha, “Endodontic management of four rooted mandibular first premolar,” Journal of Conservative Dentistry, vol. 16, pp. 87–89, 2013.

Y. Du, A. H. Lee, and C. Zhang, “Mandibular first premolar with four canals,” Journal of Investigative and Clinical Dentistry, vol. 4, pp. 64–66, 2013.

N. Kararia and V. Kararia, “Root canal treatment of a mandibular second premolar with atypical canal pattern,” Journal of Conservative Dentistry, vol. 15, pp. 392–394, 2012.

L. Holtzman, “Root canal treatment of mandivular second premolar with four root canals: a case report,” International Endodontic Journal, vol. 31, no. 5, pp. 364–366, 1998.

M. Wong, “Four root canals in a mandibular second premolar,” Journal of Endodontics, vol. 17, no. 3, pp. 125–126, 1991.

E. Macri and O. Zmener, “Five canals in a mandibular second premolar,” Journal of Endodontics, vol. 26, no. 5, pp. 304–305, 2000.

M. Goswami, S. Chandra, S. Chandra, and S. Singh, “Mandibular premolar with two roots,” Journal of Endodontics, vol. 23, no. 3, p. 187, 1997.

R. Prakash, S. Nandini, S. Ballal, S. Kumar, and D. Kandaswamy, “Two-rooted mandibular second premolars: case report and survey,” Indian Journal of Dental Research, vol. 19, no. 1, pp. 70–73, 2008.

T. Rödig and M. Hülsmann, “Diagnosis and root canal treatment of a mandibular second premolar with three root canals,” International Endodontic Journal, vol. 36, no. 12, pp. 912–919, 2003.

R. J. G. De Moor and F. L. G. Calberson, “Root canal treatment in a mandibular second premolar with three root canals,” Journal of Endodontics, vol. 31, no. 4, pp. 310–313, 2005.

M. Lotfi, S. Vosoughhosseini, V. Zand, A. Fatemi, V. Shyezadeh, and B. Ranjkesh, “A mandibular second premolar with three canals and atypical orifices,” Journal of Oral Science, vol. 50, no. 3, pp. 363–366, 2008.

L. R. Soares, M. Arruda, M. P. de Arruda et al., “Diagnosis and root canal treatment in a mandibular premolar with three canals,” Brazilian Dental Journal, vol. 20, no. 5, pp. 424–427, 2009.

N. Shokouhinejad, “Root canal re-treatment of a mandibular second premolar with three root canals: a case report,” Australian Endodontic Journal, vol. 35, no. 3, pp. 180–182, 2009.

C. Aguiar, D. Mendes, A. Câmara, and J. Figueiredo, “Endodontic treatment of a mandibular second premolar with three root canals,” Journal of Contemporary Dental Practice, vol. 11, no. 2, pp. 78–84, 2010.

S. M. Bram and R. Fleisher, “Endodontic therapy in a mandibular second biscupid with four canals,” Journal of Endodontics, vol. 17, no. 10, pp. 513–515, 1991.

K. S. Al-Fouzan, “The microscopic diagnosis and treatment of a mandibular second premolar with four canals,” International Endodontic Journal, vol. 34, no. 5, pp. 406–410, 2001.

J. S. Rhodes, “A case of unusual anatomy: a mandibular second premolar with four canals,” International Endodontic Journal, vol. 34, no. 8, pp. 645–648, 2001.

G. N. Tzanetakis, T. A. Lagoudakos, and E. G. Kontakiotis, “Endodontic treatment of a mandibular second premolar with four canals using operating microscope,” Journal of Endodontics, vol. 33, no. 3, pp. 318–321, 2007.

S. Shalavi, Z. Mohammadi, and M. Abdolrazzaghi, “Root canal treatment of maxillary and mandibular three-rooted premolars: case reports,” Iranian Endodontic Journal, vol. 7, pp. 161–164, 2012.

H. Mokhtari, M. Niknami, and V. Zand, “Managing a mandibular second premolar with three-canal and taurodontism: a case report,” Iranian Endodontic Journal, vol. 8, pp. 25–28, 2013.

Y. Shapira and P. Delivanis, “Multiple-rooted mandibular second premolars,” Journal of Endodontics, vol. 8, no. 5, pp. 231–232, 1982.

G. S. Sachdeva, S. Ballal, V. Gopikrishna, and D. Kandaswamy, “Endodontic management of a mandibular second premolar with four roots and four root canals with the aid of spiral computed tomography: a case report,” Journal of Endodontics, vol. 34, no. 1, pp. 104–107, 2008.

E.-T. Farmakis, “Four-rooted mandibular second premolar,” Australian Endodontic Journal, vol. 34, no. 3, pp. 126–128, 2008.

G. R. Hartup, “Dens invaginatus type III in a mandibular premolar,” General Dentistry, vol. 45, no. 6, pp. 584–587, 1997.

C. M. Bramante, S. M. G. de Sousa, and S. M. R. Tavano, “Dens invaginatus in mandibular first premolar,” Oral Surgery, Oral Medicine, Oral Pathology, vol. 76, no. 3, p. 389, 1993.

S. M. Tavano, S. M. de Sousa, and C. M. Bramante, “Dens invaginatus in first mandibular premolar,” Endodontics & Dental Traumatology, vol. 10, no. 1, pp. 27–29, 1994.

S. Aryanpour, P. Bercy, and J.-P. Van Nieuwenhuysen, “Endodontic and periodontal treatments of a geminated mandibular first premolar,” International Endodontic Journal, vol. 35, no. 2, pp. 209–214, 2002.

K. Er, A. Kuştarci, Ü. Özan, and T. Taşdemir, “Nonsurgical endodontic treatment of dens invaginatus in a mandibular premolar with large periradicular lesion: a case report,” Journal of Endodontics, vol. 33, no. 3, pp. 322–324, 2007.

E. Canger, S. Kayipmaz, and P. Celenk, “Bilateral dens invaginatus in the mandibular premolar region,” Indian Journal of Dental Research, vol. 20, no. 2, pp. 238–240, 2009.

E. T. Koh, T. R. Pitt Ford, S. P. Kariyawasam, N. N. Chen, and M. Torabinejad, “Prophylactic treatment of dens evaginatus using mineral trioxide aggregate,” Journal of Endodontics, vol. 27, no. 8, pp. 540–542, 2001.

I. Tsesis, N. Steinbock, E. Rosenberg, and A. Y. Kaufman, “Endodontic treatment of developmental anomalies in posterior teeth: treatment of geminated/fused teeth—report of two cases,” International Endodontic Journal, vol. 36, no. 5, pp. 372–379, 2003.

R. Sathish Muthukumar, S. Arunkumar, and K. Sadasiva, “Bilateral fusion of mandibular second premolar and supernumerary tooth: a rare case report,” Journal of Oral and Maxillofacial Pathology, vol. 16, no. 1, pp. 128–130, 2012.

E. O. Demiryürek, N. Gonulol, and B. Bulucu, “Endodontic treatment of a taurodontic premolar with five canals,” Australian Endodontic Journal, vol. 39, pp. 81–84, 2013.

A. Kusiak, J. Sadlak-Nowicka, J. Limon, and B. Kochańska, “Root morphology of mandibular premolars in 40 patients with Turner syndrome,” International Endodontic Journal, vol. 38, no. 11, pp. 822–826, 2005.

R. Scott and C. Turner II, The Anthropology of Modern Human Teeth, Cambridge University Press, Cambridge, UK, 2000.

E. J. Carns and A. E. Skidmore, “Configurations and deviations of root canals of maxillary first premolars,” Oral Surgery, Oral Medicine, Oral Pathology, vol. 36, no. 6, pp. 880–886, 1973.

B. H. Seidberg, M. Altman, J. Guttuso, and M. Suson, “Frequency of two mesiobuccal root canals in maxillary permanent first molars,” The Journal of the American Dental Association, vol. 87, no. 4, pp. 852–856, 1973.

M. C. Madeira and S. Hetem, “Incidence of bifurcations in mandibular incisors,” Oral Surgery Oral Medicine and Oral Pathology, vol. 36, no. 4, pp. 589–591, 1973.

I. Kaffe, A. Kaufman, M. M. Littner, and A. Lazarson, “Radiographic study of the root canal system of mandibular anterior teeth,” International Endodontic Journal, vol. 18, no. 4, pp. 253–259, 1985.

R. Ordinola-Zapata, C. M. Bramante, M. H. Villas-Boas, B. C. Cavenago, M. A. Duarte, and M. A. Versiani, “Morphologic micro-computed tomography analysis of mandibular premolars with three root canals,” Journal of Endodontics, vol. 39, no. 9, pp. 1130–1135, 2013.

F. M. Pucci and R. Reig, Conductos Radiculares. Vol. I, Editorial Medico-Quirurgica, Buenos Aires, Argentina, 1944.

B. M. Cleghorn, W. H. Christie, and C. C. S. Dong, “The root and root canal morphology of the human mandibular first premolar: a literature review,” Journal of Endodontics, vol. 33, no. 5, pp. 509–516, 2007.

B. M. Cleghorn, W. H. Christie, and C. C. S. Dong, “The root and root canal morphology of the human mandibular second premolar: a literature review,” Journal of Endodontics, vol. 33, no. 9, pp. 1031–1037, 2007.