Salinomycin as a Drug for Targeting Human Cancer Stem Cells

Cancer stem cells (CSCs) represent a subpopulation of tumor cells that possess self-renewal and tumor initiation capacity and the ability to give rise to the heterogenous lineages of malignant cells that comprise a tumor. CSCs possess multiple intrinsic mechanisms of resistance to chemotherapeutic drugs, novel tumor-targeted drugs, and radiation therapy, allowing them to survive standard cancer therapies and to initiate tumor recurrence and metastasis. Various molecular complexes and pathways that confer resistance and survival of CSCs, including expression of ATP-binding cassette (ABC) drug transporters, activation of the Wnt/β-catenin, Hedgehog, Notch and PI3K/Akt/mTOR signaling pathways, and acquisition of epithelial-mesenchymal transition (EMT), have been identified recently. Salinomycin, a polyether ionophore antibiotic isolated from Streptomyces albus, has been shown to kill CSCs in different types of human cancers, most likely by interfering with ABC drug transporters, the Wnt/β-catenin signaling pathway, and other CSC pathways. Promising results from preclinical trials in human xenograft mice and a few clinical pilote studies reveal that salinomycin is able to effectively eliminate CSCs and to induce partial clinical regression of heavily pretreated and therapy-resistant cancers. The ability of salinomycin to kill both CSCs and therapy-resistant cancer cells may define the compound as a novel and an effective anticancer drug.


Introduction
The demonstration of cancer stem cells (CSCs) in a variety of human malignant tumors, including cancers of the blood, breast, brain, bone, skin, liver, lung, bladder, ovary, prostate, colon, pancreas, and head and neck has led to the conceptual hypothesis that tumors, like physiologic proliferative tissues, can be hierarchically organized and propagated by limited numbers of stem cells [1][2][3][4][5][6][7][8]. According to a consensus definition, these CSCs are cells within a tumor that possess the capacity to self-renew and to give rise to the heterogeneous lineages of cancer cells that comprise the tumor [9]. CSCs can be defined experimentally by their ability to recapitulate the generation of a continuously growing tumor in serial xenotransplantation approaches [9]. It is not yet entirely clear whether such tumorigenic cells identified and isolated from hematopoietic and solid tumors by virtue of their expression of specific cell surface markers [5,7] are the real "stem cells" of the tumor, but, although not readily testable, the CSC concept of carcinogenesis and tumor maintenance is fairly accepted to date [3][4][5][6][7]. Moreover, recent studies provide evidence for the existence and relevance of CSCs in clinical therapeutic situations [10][11][12].

The Challenge of Targeting CSCs and Their Progeny
According to the cancer stem cell concept of carcinogenesis [1,3,4,[7][8][9]73], CSCs represent novel and translationally relevant targets for cancer therapy, and the identification, development, and therapeutic use of compounds and drugs that selectively target CSCs are a major challenge for future cancer treatment [5,65,[74][75][76]. However, the goal for any CSC-directed therapy should be the eradication of all CSCs in a patient, and the efficacy of single agents targeting CSCs may be limited by several factors. CSCs represent a heterogenous population that may not be homogeneously sensitive to a given anti-CSC agent [8,[77][78][79], and, under the selection pressure of agents targeting CSCs, therapy-resistant CSC clones may emerge [80]. Therefore, the eradication of all CSCs will likely require targeting of more than one intrinsic pathway operating in CSCs to reduce the probability of escape mutants [74,[81][82][83]. Moreover, agents causing tumor regression in advanced stages of cancer likely reflect effects on the bulk tumor population but may have minimal effect on the CSC population. In contrast, a CSC-specific therapy would show modest effect on tumor growth of the bulk tumor population in advanced stages of cancer but may have substantial clinical benefit in early stages of cancer as well as in neoadjuvant and adjuvant clinical settings [75]. Ultimately, cure of cancer will require the eradication of all malignant cells within a patient's cancer: CSCs and their progeny. Recent data obtained in vitro and in xenograft mice bearing human cancers indicate that CSC targeting agents are most effective in eradicating CSCs and their progeny when these agents are combined with conventional cytostatic drugs and/or novel tumor-targeted drugs [84][85][86][87][88][89][90][91][92][93][94][95]. Therefore it will be important and promising to combine in sophisticated clinical settings CSC targeting agents with novel tumortargeted drugs and conventional cytotoxic drugs. Such combinations may act in concert to eradicate CSCs, more differentiated progenitors, and bulk tumor cells in cancer patients [87,88,[96][97][98][99][100][101].
Although these compounds and drugs have been shown to effectively target signaling pathways and/or molecules selectively operating in CSCs, some of them are also capable of killing other types and subpopulations of cancer cells, which do not display CSC properties. In particular, the biomolecules salinomycin and parthenolide as well as the biguanide metformin have been demonstrated to induce apoptosis in various types of human cancer cells [108,123,124], suggesting that these compounds may contribute to the eradication of cancer more effectively than compounds targeting either CSCs or regular cancer cells. Moreover, the ionophore antibiotic salinomycin seems to have even extended capabilities of eliminating cancer (Table 1), because this compound has been demonstrated to effectively target regular cancer cells [16,[125][126][127], highly multidrug and apoptosis-resistant cancer cells [16,85,125], and CSCs [16,84,87,88,[128][129][130][131].

From Broiler to Bedside: A Brief History of Salinomycin
During the course of a screening program for new antibiotics in the early seventies, Miyazaki and colleagues from the research division of Kaken Chemicals Co., Ltd., Tokyo, Japan isolated a new biologically active substance from the culture broth of Streptomyces albus strain no. 80614 that was termed salinomycin [102]. The salinomycinproducing organism was detected in and isolated from a soil sample collected at Fuji City, Shizuoka Prefecture, Japan, taxonomically classified as a member of the Streptomyces albus genus (ROSSI-DORIA) WAKSMAN and HENRICI and designated as the strain no. 80614 [102,135]. The production of salinomycin was carried out by tank fermentation, filtration of the culture broth of Streptomyces albus strain no. 80614, purification by column chromatography on alumina/silica gel and subsequent elution. The eluate was then concentrated in vacuo, dried, and finally crystallized. By this isolation procedure, salinomycin was obtained in the form of colourless prism of the sodium salt [102]. Subsequently, it was demonstrated that salinomycin exhibits antimicrobial activity against Gram-positive bacteria including Bacillus subtilis, Staphylococcus aureus, Micrococcus flavus, Sarcina lutea, Mycobacterium spp., some filamentous fungi, Plasmodium falciparum, and Eimeria spp., protozoan parasites responsible for the poultry disease First demonstration that salinomycin induces apoptosis and overcomes ABC transporter-mediated multidrug resistance in human CSCs. [16] Lung CSCs Human lung adenocarcinoma A549 cells expressing ALDH1, Oct-4, Nanog, and Sox2.
Salinomycin inhibits tumorsphere formation, induces apoptosis in osteosarcoma CSCs, sensitizes them to conventional cytostatic drugs, and downregulates Wnt/β-catenin signaling in the cells. [132] 4 Journal of Biomedicine and Biotechnology First demonstration that salinomycin inhibits Wnt signaling in human cancer cells (CLL cells).
[126] Increased clinical antitumor activity of salinomycin in combination with erlotinib. [105] 6 Journal of Biomedicine and Biotechnology coccidiosis [102,136,137]. The chemical structure of salinomycin was determined by X-ray crystallographic analysis, which revealed that salinomycin was a new member of the family of the monocarboxylic polyether antibiotics [103], (Figure 1). Because other polyether antibiotics, such as monensin and lasalocid, had previously been shown to exhibit effective antimicrobial activities against Eimeria spp., the anticoccidial estimation of salinomycin was carried out with chickens infected with Eimeria tenella oocysts. Salinomycin was effective in reducing the mortality of chickens from coccidiosis and in increasing average body weight of treated infected chickens [102]. Thus, a patent had been issued for the use of salinomycin to prevent coccidiosis in poultry [138], and, up to today, salinomycin is used in broiler batteries and other livestock as an anticoccidial drug and is also fed to ruminants and pigs to improve nutrient absorption and feed efficiency [136,[139][140][141][142].
In addition, salinomycin had early been shown to act in different biological membranes, including cytoplasmic and mitochondrial membranes, as a monovalent cation ionophore with strict selectivity for alkali ions and a strong preference for K + [143], thereby promoting mitochondrial and cytoplasmic K + efflux and inhibiting oxidative phosphorylation [144,145]. Similar to the monocarboxylic polyether antibiotic monensin, which exhibits complex cardiovascular effects due to its transport of Na + across biological membranes [146], salinomycin had been demonstrated by Pressman and colleagues as a positive ionotropic and chronotropic agent that increased cardiac output, left ventricular systolic pressure, heart rate, mean arterial pressure, coronary artery vasodilatation and blood flow, and plasma catecholamine concentration [147]. These results had been obtained in experiments with mongrel dogs that had received a single intravenous injection of 150 μg·kg −1 salinomycin [147]. The total synthesis of the salinomycin molecule was achieved in 1998 [148], and amide derivatives of salinomycin with activity against Gram-positive bacteria and methicillinresistant Staphylococcus aureus have been synthesized very recently [149].
However, for several reasons, salinomycin has never been established as a drug for human diseases until now. In particular, several reports and studies published in the last three decades revealed a considerable toxicity of salinomycin in mammals, such as horses, pigs, cats, and alpacas after accidental oral or inhalative intake [150][151][152][153][154]. A case of an accidental inhalation and swallowing of about 1 mg·kg −1 salinomycin by a 35-year-old male human revealed severe acute and chronic salinomycin toxicity with acute nausea, photophobia, leg weakness, tachycardia and blood pressure elevation and a chronic (day 2 to day 35) creatine kinase elevation, myoglobinuria, limb weakness, muscle pain, and mild rhabdomyolysis [155]. Risk assessment data recently published by the European Food Safety Authority declare an acceptable daily intake (ADI) of 5 μg·kg −1 salinomycin for humans, because daily intake of more than 500 μg·kg −1 salinomycin by dogs leads to neurotoxic effects, such as myelin loss and axonal degeneration [156]. In view of this considerable toxicity in mammals, salinomycin has only been used for more than 30 years as a coccidistat and growth promoter in livestock, but, in terms of human drug development salinomycin rested in dormancy. Until in 2009 a seminal study by Gupta and colleagues revealed that salinomycin selectively eliminates human breast CSCs in mice [128]. A follow-up publication demonstrated that salinomycin induces massive apoptosis in human cancer cells of different origin that display multiple mechanisms of drug and apoptosis resistance [125]. Based on these findings, salinomycin was therapeutically applicated "first-in-man" in 2010, in the context of a pilote clinical trial with a small cohort of patients with metastatic breast, ovarian, and head and neck cancers [104]. Intravenous administration of 200-250 μg·kg −1 salinomycin every second day for three weeks resulted in partial regression of tumor metastasis and showed only minor acute and long-term side effects, but no severe acute and long-term side effects observed with conventional chemotherapeutic drugs [104]. Consequently, a phase I/II clinical trial with VS-507 (a proprietary formulation of salinomycin produced by Verastem Inc., Cambridge, MA, USA) in patients with triple negative breast cancer is envisioned to start in 2013. However, it is actually unclear whether salinomycin will be used routinely for the treatment of cancer patients in the very next years.

Salinomycin as a Drug for Targeting CSCs
It was a great surprise when Gupta and colleagues showed in 2009 that salinomycin selectively kills human breast CSCs [128]. In a sophisticated experimental approach, the authors used triple oncogenic transformed and immortalized human mammary epithelial cells (termed HMLER), in which knockdown of E-cadherin by RNA interference resulted in the generation of cells undergoing epithelial-mesenchymal transition (EMT), a latent embryonic program that can endow cancer cells with migratory, invasive, self-renewal and drug resistance capabilities [157][158][159][160]. These human breast cancer stem-like cells (termed HMLER-shEcad) that Journal of Biomedicine and Biotechnology 7 displayed characteristic properties of CSCs were capable of forming tumorspheres in suspension cultures (a standard clonogenic assay for the detection of self-renewal of CSC, [9]), showed high and low expression of CD44 and CD24, respectively, and exhibited resistance to chemotherapeutic drugs and cytotoxic agents, such as paclitaxel, doxorubicin, actinomycin D, campthotecin, and staurosporine [128]. In a high-throughput screening approach, about 16,000 compounds from chemical libraries, including biological molecules and natural extracts with known bioactivity, were tested for activity and toxicity against HMLER-shEcad cells and control cells that had not undergone EMT. From a pool of 32 promising candidates, only one compound markedly and selectively reduced the viability of breast cancer stem-like HMLER-shEcad cells: salinomycin. It was further demonstrated that salinomycin, in contrast to the anti-breast cancer drug paclitaxel, selectively reduces the proportion of CD44 high /CD24 low CSCs in cultures of mixed populations of HMLER-shEcad cells and control cells that had not undergone EMT. In addition, pretreatment of HMLER-shEcad cells with salinomycin resulted in inhibition of HMLER-shEcad-induced tumorsphere formation, which was not observed after pretreatment of the cells with paclitaxel [128]. Using comparative global gene expression profiling, it was shown that, in CD44 high /CD24 low HMLER cells, salinomycin, but not paclitaxel, was capable of changing gene expression signatures characteristic of breast CSCs or mammary epithelial progenitor cells isolated from human breast cancers. In particular, expression of genes that inversely correlates with metastasis-free survival, overall survival, and clinical outcome of breast cancer patients [161,162] was downregulated by salinomycin. Expression of a set of genes that promote the expansion of mammary epithelial stem cells and the formation of tumorspheres [163] was also markedly downregulated by salinomycin but not by paclitaxel. In contrast, genes involved in mammary epithelial differentiation that encode membrane-associated and secreted proteins of the extracellular matrix were upregulated by salinomycin [128]. Finally, as a proof of principle, it was demonstrated that salinomycin inhibits the ability of breast CSCs to form tumors in mice. Pretreatment of HMLER cells for 7 days with salinomycin and subsequent serial limiting dilution and injection of the cells into NOD/SCID mice resulted in a >100-fold decrease in tumor-seeding ability, relative to pretreatment of the cells with paclitaxel. Finally, salinomycin treatment of NOD/SCID mice with human breast cancers (xenograft mice) resulted in a reduction of the tumor mass and metastasis, and explanted tumors showed a reduced number of breast CSCs as well as an increased epithelial differentiation [128].
According to the primary finding that salinomycin induces massive apoptosis in human cancer cells that display different mechanisms of drug and apoptosis resistance [125], a subsequent study demonstrated that salinomycin is able to overcome ABC transporter-mediated multidrug resistance and apoptosis resistance in human acute myeloid leukemia stem cells (AML SCs) [16]. One of the most important mechanism of drug resistance in leukemia SCs and other CSCs is the expression of ABC transporters belonging to a highly conserved superfamily of transmembrane proteins capable of exporting a wide variety of macromolecules and structurally unrelated chemotherapeutic drugs from the cytosol, thereby conferring multidrug resistance, which is a major obstacle in the success of cancer chemotherapy [14,17,164,165]. As demonstrated in the study, expression of functional ABC transporters, such as P-glycoprotein/MDR1, ABCG2/BCRP, and ABCC11/MRP8 in human KG-1a AML SCs confers resistance of the cells to various chemotherapeutic drugs, including cytosine arabinoside, doxorubicin, gemcitabine, 5-fluorouracil, topotecan, etoposide, and bortezomib, but not to salinomycin, which was capable of inducing massive apoptosis in KG-1a AML SCs [16]. Of note, salinomycin did not permit long-term adaptation and development of resistance of KG-1a AML SCs to apoptosis-inducing concentrations of salinomycin, whereas the cells could readily be adapted to survive and to proliferate in the presence of initially apoptosis-inducing concentrations of doxorubicin and bortezomib [16], (Figure 2).
These findings strongly suggest that salinomycin is capable of targeting breast CSCs and AML SCs, and a series of recent studies show similar effects of salinomycin in other types of CSCs. In gastrointestinal stromal tumors (GISTs), the most common gastrointestinal sarcomas, a subpopulation of cells expressing CD44, CD34, and low Kit (activating stem cell factor receptor) have been identified as cells with self-renewal and tumorigenic capabilities [84]. These Kit low CD44+ CD34+ CSCs are resistant to inhibition of proliferation by imatinib, a tyrosine kinase inhibitor targeting oncogenic kit signaling that is commonly used in the treatment of metastatic GIST [84,166]. By contrast, salinomycin nearly completely inhibited the proliferation of Kit low CD44+ CD34+ CSCs without causing apoptosis, and salinomycin also promoted differentiation of the cells, as evidenced by the occurrence of multipolarity and a fibroblastlike morphology [84]. However, a combined treatment of the cells with imatinib and salinomycin caused a significantly greater inhibition of proliferation than salinomycin alone [84]. Thus, the study demonstrates that salinomycin is able to inhibit proliferation and to induce differentiation of GIST CSCs and also suggests that a combination of salinomycin and imatinib may provide therapeutic benefit in patients with GIST.
Similar results were obtained with CD44+ CD24− ALDH1+ breast CSCs isolated from the human breast cancer cell line MCF-7. In CD44+ CD24− ALDH1+ MCF-7-derived breast CSCs, salinomycin was capable of markedly reducing the tumorsphere formation of the cells and the percentage of ALDH1+ expressing cells by nearly 50-fold [85]. Treatment of the cells with salinomycin as well as combined treatment with the cytostatic drug doxorubicin and salinomycin, but not treatment with doxorubicin alone, reduced the cloning efficiency by 10-30-fold and markedly increased apoptosis in CD44+ CD24− ALDH1+ breast CSCs [85]. Similar results were obtained recently with MCF-7-derived breast CSCs and HER2-expressing breast cancer cells that were more effectively killed by the combination of salinomycin and the anti-HER2 antibody trastuzumab than by salinomycin or trastuzumab alone [86], providing further evidence that salinomycin alone and particularly in combination with conventional anticancer drugs effectively targets CSCs.
Salinomycin has recently been shown to target CSCs in different types of human cancers, including gastric cancer [131], lung adenocarcinoma [130], osteosarcoma [132], colorectal cancer [129], squamous cell carcinoma (SCC) [133], and prostate CSCs [134], suggesting that salinomycin may be effective in CSCs of many, if not all, types of human cancers, although it is currently not known whether all cancers contain subpopulations of CSCs.
In ALDH1+ gastric CSCs, which displayed resistance to the conventional chemotherapeutic drugs 5-fluorouracil and cisplatin, salinomycin effectively inhibited tumorsphere formation, proliferation, and viability of the cells [131]. Similar results were obtained in ALDH1+ CSCs derived form lung adenocarcinoma cells [130] and in osteosarcoma CSCs [132]. In colorectal cancer cells and in squamous cell carcinoma (SCC) cells, salinomycin, but not oxaliplatin or cisplatin, was able to significantly reduce the proportion of CSCs, as detected in tumorsphere assays [129,133] and in human SCC xenograft mice [133].
As noted above, cure of cancer requires the eradication of all cell types within a cancer, namely, CSCs, more differentiated progenitors and the bulk tumor cell population that might be achieved by combining CSC targeting agents with conventional cytotoxic drugs [74,75,83]. This hypothesis received substantial support from recent studies showing that salinomycin in combination with a conventional cytotoxic drug eradicates human cancers in xenograft mice much more efficiently than the single agent [87,88]. In particular, salinomycin inhibited the growth of CD133+ pancreatic CSCs in tumorsphere formation assays, while the cytotoxic drug gemcitabine, a nucleoside analog commonly used in the treatment of metastatic pancreatic cancer, induced marked apoptosis in non-CSC 133-pancreatic cancer cells [87]. Consistently, salinomycin combined with gemcitabine eradicated human pancreatic cancer in xenograft mice much more efficiently than either salinomycin or gemcitabine alone [87]. Similar results were obtained in a study using CD44+ CD24− breast CSCs sorted from the human breast cancer cell line MCF-7 [88]. Salinomycin more efficiently inhibited the proliferation of CD44+ CD24− breast CSCs than of the parental MCF-7 cells, and salinomycin was able to induce significant tumor regression and to reduce the number of CD44+ CD24− breast CSCs in tumors established by MCF-7 cells in xenograft mice [88]. Of note, salinomycin in combination with paclitaxel almost completely eradicated the MCF-7 tumors in xenograft mice [88].
There is growing evidence that salinomycin not only targets CSCs, but also kills more differentiated non-CSC tumor cells and, most importantly, cancer cells that display efficient mechanisms of resistance to cytotoxic drugs, radiation, and induction of apoptosis. Salinomycin has been shown to induce massive apoptosis in acute T-cell leukemia cells [125] and chronic lymphocytic leukemia cells [126] isolated from leukemia patients but failed to induce apoptosis in normal human T cells and peripheral blood lymphocytes isolated from healthy individuals [125,126]. In different human cancer cells exhibiting resistance to cytotoxic drugs, radiation, and apoptosis, salinomycin has been demonstrated to induce significant apoptosis and to increase DNA damage [89,125,167]. As in the case of breast CSCs, pancreatic CSCs, and GIST CSCs [84][85][86][87][88], salinomycin is able to enhance in regular cancer cells the cytotoxic effects of conventional cancer drugs and novel tumor-targeted drugs, such as doxorubicin, gemcitabine, etoposide, paclitaxel, docetaxel, vinblastine, and trastuzumab [86,87,89,90], envisioning a central role for salinomycin-based combination therapies in the future treatment of cancer [109].

Mechanisms of Salinomycin's Action against CSCs
Although the exact mechanisms underlying the elimination of CSCs by salinomycin remain poorly understood, recent work has contributed to an increased understanding of some mechanisms and modes of action of salinomycin in human CSCs and cancer cells.

Induction of Apoptosis and Cell Death.
It has been shown that salinomycin induces apoptosis in CSCs of different origin [16,85,132], but the particular mechanisms of apoptosis induction by salinomycin in CSCs remain unclear and may differ among the cell type, as demonstrated for cancer cells of different origin [89,90,125,127]. In particular, salinomycin-induced apoptosis in human KG-1a AML SCs and Molt-4 T-cell leukemia cells is caspase independent [16,125], whereas salinomycin has been shown to activate the mitochondrial pathway of apoptosis and the caspase-3-mediated cleavage of PARP in human PC-3 prostate cancer cells [127]. Salinomycin is able to generate reactive oxygen species (ROS) in prostate cancer cells [127,134]. Interestingly, salinomycin-mediated generation of ROS leads to apoptosis in PC-3 prostate cancer cells [127], whereas VCaP and LNCaP prostate cancer cells display growth inhibition but not apoptosis induction in response to salinomycin-induced ROS generation [134]. Salinomycininduced apoptosis is not accompanied by cell cycle arrest in human Molt-4 leukemia cells [125] that is rather unusual, because, in many types of cancer cells, cell cycle arrest precedes apoptosis induced by different agents. Notably, to date there is only one report available showing that salinomycin induces rather nonapoptotic cell death than apoptotic cells death, as demonstrated by the loss of viability and weak cleavage of PARP in human Hs578T breast cancer cells exposed to salinomycin [90]. However, combination treatment of the cells with salinomycin and paclitaxel or salinomycin and docetaxel resulted in the induction of marked cleavage of PARP and induction of apoptosis that was not observed after treatment of the cells with either drug alone [90]. These data suggest that induction of apoptosis or nonapoptotic cell death by salinomycin in cancer cells seems to be dependent on the particular cell type investigated, and the detailed mechanisms of salinomycininduced apoptosis and nonapoptotic cell death in cancer cells are far from being clear to date. Salinomycin has been shown to induce apoptosis in CSCs derived from human acute myeloid leukemia cells [16], breast cancer cells [85], and osteosarcoma cells [132]. Whether salinomycin is able to induce apoptosis or rather nonapoptotic cell death in CSCs derived from other types of human cancers is currently unknown.

Interference with ABC Transporters.
It is evident that salinomycin is refractory to the action of ABC transporters since salinomycin is able to overcome ABC transporter-mediated multidrug resistance in AML SCs [16]. Salinomycin is a 751 kDa transmembrane K + ionophore which is rapidly embedded in biological membranes such as the cytoplasmic and mitochondrial membrane [144]. ABC transporters also constitute transmembrane macromolecules that extrude a variety of substrates from the cytosol [14,17,164]. Therefore it is unlikely that salinomycin becomes a substrate of ABC transporters [16]. Moreover, salinomycin has been demonstrated to be a potent inhibitor of the ABC transporter P-glycoprotein/MDR1 in different cancer cells [167,168].

Activation of the Wnt/β-Catenin Signaling Pathway.
Constitutive activation of the Wnt/β-catenin signaling pathway is essential for maintenance, clonogenicity and other specific characteristics of CSCs [18-21, 169, 170] and, most importantly, Wnt/β-catenin signaling confers resistance of CSCs to radiation [171,172] and to anticancer drugs [18,19]. Salinomycin, however, has been shown to inhibit in chronic lymphocytic leukemia cells proximal Wnt signaling by reducing the levels of the Wnt coreceptor LRP6 and by downregulating the expression of the Wnt target genes LEF1, cyclin D1, and fibronectin, finally leading to apoptosis [126].

Inhibition of Oxidative Phosphorylation.
Most cancer cells rely more on aerobic glycolysis than on oxidative phosphorylation (the Warburg effect) [173], but, for instance, malignant transformation of human mesenchymal stem cells is linked to an increase of oxidative phosphorylation [174]. Moreover, glioma CSCs have been shown to mainly rely on oxidative phosphorylation [175], suggesting that inhibition of metabolic pathways, such as oxidative phosphorylation, is a promising strategy to target CSCs. In this context, salinomycin is known to inhibit oxidative phosphorylation in mitochondria [144] that may contribute to the elimination of CSCs by salinomycin.

Cytoplasmic and Mitochondrial K + Efflux.
Salinomycin is a K + ionophore that interferes with transmembrane K + potential and promotes the efflux of K + from mitochondria and cytoplasm [143][144][145]. Expression of K + channels has been documented in CD34+/CD38− AML SCs and in CD133+ neuroblastoma CSCs, but not in their nontumorigenic counterparts [176,177], suggesting an important role of K + channels in CSC maintenance. Moreover, a decrease in intracellular K + concentration by pharmacological induction of K + efflux is directly linked to the induction of apoptosis and cytotoxicity in cancer cells [178,179], suggesting that mitochondrial and cytoplasmic K + efflux induced by salinomycin leads to apoptosis in CSCs.
6.6. Differentiation of CSCs. Finally, salinomycin is able to promote differentiation of CSCs and to induce epithelial reprogramming of cells that had undergone EMT [84,128]. This is in concert with the finding that salinomycin upregulates the expression of genes involved in mammary epithelial differentiation [128]. Thus, salinomycin might target and eliminate CSCs by multiple mechanisms of which only a few are currently known. Future research may uncover an increasing number of relevant mechanisms of targeting CSCs by salinomycin.  (Figure 4). The patient refused a further therapy containing erlotinib due to marked adverse effects of erlotinib (fatigue, nausea, anorexia, and inappetance) experienced during initial therapy with salinomycin combined with erlotinib. Therefore, the patient received 12 intravenous administrations of 250 μg·kg −1 salinomycin every second day, without addition of erlotinib. This monotherapy with salinomycin resulted in stable disease and no progression for 4 months, as determined by clinical inspection of the local tumor and no significant changes of SCC serum levels ( Figure 4). As observed in case 1, intravenous therapy with 250 μg·kg −1 salinomycin every second day resulted in minor acute side effects, including tachycardia and mild tremor for 30-60 min. after administration but lacked severe and long-term side effects, such as myelodepression, neutropenia, alopecia, nausea and vomiting, or gastrointestinal, thromboembolic, and neurological side effects.

Conclusions
Work from the last few years highlights the possibility of selectively targeting CSCs, which are regarded as the major culprits in cancer. However, although the rather novel CSC concept of carcinogenesis is fairly accepted to date, more classical mechanisms and driving forces of carcinogenesis, including genome instability, epigenetic modifications, first oncogenic hit(s), clonal evolution, replicative immortality, invasion and metastasis, immune evasion, reprogramming of energy metabolism, and most probably, a complex interplay of all of these mechanisms must be considered as a basis for defining carcinogenesis and cancer in general [73,80,[180][181][182][183][184][185]. Nevertheless, in line with the CSC concept of carcinogenesis [1,3,4,[7][8][9]73], CSCs constitute adequately characterized cells and represent novel and translationally relevant targets for cancer therapy [5,65,74,75]. Significant advances have been made recently in the discovery, development, and validation of novel compounds and drugs that target CSCs, and the future clinical use of these novel agents may represent a powerful strategy for eradicating CSCs in cancer patients, thereby preventing tumor recurrence and metastasis, and, hopefully, contributing to the cure of cancer. There is growing consensus that conventional cytotoxic drugs are unable to eradicate CSCs [5,12,64,74], and, more disturbing, CSCs can be even    selectively enriched by these drugs, as demonstrated in breast cancer patients receiving systemic chemotherapy comprising conventional cytotoxic drugs [10,67,68,186]. Moreover, many novel tumor-targeted drugs, including tyrosine kinase inhibitors and monoclonal antibodies raised against tumorspecific cell surface proteins, also fail to eliminate CSCs [70,95,[187][188][189], so that there is an urgent need for novel compounds and drugs that effectively target CSCs for the use in elaborated clinical settings and preferably in combination with conventional cytostatic drugs and novel tumor-targeted agents.
In this context, one promising candidate drug is the ionophor antibiotic salinomycin, which has recently been documented to effectively eliminate CSCs in different types of human cancers in vitro and in xenograft mice bearing human cancers [87,88,128,133]. To date it is not entirely clear by which mechanisms salinomycin eliminates CSCs, but it is important to note that salinomycin, in combination with conventional cytotoxic drugs, is much more effective in eradicating human cancers in xenograft mice than the single agent alone [87,88]. This is in accord with the postulation that efficient cancer therapy should target all cancer cell populations, including CSCs, more differentiated progenitors, and bulk tumor cells that might be achieved by combining CSC targeting agents with conventional cytotoxic drugs, novel tumor-targeted drugs, and radiation therapy [5,74,82].
Importantly, salinomycin is not only able to kill CSCs, but also regular tumor cells and highly indolent tumor cells displaying resistance to cytotoxic drugs, radiation, and induction of apoptosis [85,125,167], hence salinomycin can be regarded as a triple-edged sword against cancer. This is in line with results from a few clinical pilote studies revealing that salinomycin is able to induce partial clinical regression of heavily pretreated and therapy-resistant cancers, particularly in combination with novel tumor-targeted drugs [104,105]. More work is required to define the exact mechanisms of salinomycin's action against CSCs, to estimate its long-term safety in humans, and finally, to exploit its probably huge therapeutic potential in combination with other drugs in all stages of human cancer.