BioMed Research International

Volume 2013 (2013), Article ID 723181, 7 pages

http://dx.doi.org/10.1155/2013/723181

Research Article

Essential Oils from Fruits with Different Colors and Leaves of Neomitranthes obscura (DC.) N. Silveira: An Endemic Species from Brazilian Atlantic Forest

Raquel R. Amaral,1,2 Caio P. Fernandes,1,2 Otávio P. Caramel,² Luis A. C. Tietbohl,² Marcelo G. Santos,³ José C. T. Carvalho,⁴ and Leandro Rocha1,2

¹Programa de Pós-Graduação em Biotecnologia Vegetal, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro (UFRJ), Bloco K, 2° No andar, Sala 032, Avenida Brigadeiro Trompowski s/n, 21941-590 Ilha do Fundão, RJ, Brazil

²Laboratório de Tecnologia de Produtos Naturais (LTPN), Departamento de Tecnologia Farmacêutica, Faculdade de Farmácia, Universidade Federal Fluminense (UFF), Rua Mario Viana 523, Santa Rosa, 24241-000 Niterói, RJ, Brazil

³Faculdade de Formação de Professores, UERJ, Rua Dr. Francisco Portela1470, Patronato, 24435-005 São Gonçalo, RJ, Brazil

⁴Laboratório de Pesquisa em Fármacos, Colegiado de Ciências Farmacêuticas, Universidade Federal do Amapá, Campus Universitário, Marco Zero do Equador, Rodovia Juscelino Kubitschek de Oliveira, KM-02 Bairro Zerão, 68902-280 Macapá, AP, Brazil

Received 12 September 2012; Revised 20 November 2012; Accepted 21 November 2012

Academic Editor: Fabio Ferreira Perazzo

Copyright © 2013 Raquel R. Amaral et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract.

Neomitranthes obscura (DC.) N. Silveira is an endemic plant of Brazilian Atlantic Forest and widely spread in the sandbanks of “Restinga de Jurubatiba” National Park. It is popularly known by local population as “camboim-de-cachorro” or “cambuí-preto” and recognized by its black ripe fruits. However, specimens with yellow ripe fruits were localized in the “Restinga de Jurubatiba” National Park. The aim of the present study was to evaluate chemical composition of essential oils obtained from leaves and fruits of N. obscura specimens with different fruit color (black and yellow) by GC and GC-MS. Essential oils from leaves of specimens with black and yellow fruits indicated a predominance of sesquiterpenes (81.1% and 84.8%, resp.). Meanwhile, essential oil from black fruits presented a predominance of monoterpenes (50.5%), while essential oil from yellow fruits had sesquiterpenes (39.9%) as major substances. Despite previous studies about this species, including essential oil extraction, to our knowledge this is the first report on N. obscura fruits with different colors. Our results suggest the occurrence of unless two different varieties for this species.

1. Introduction

“Restinga” is a type of habitat originated from Quaternary marine deposits and is represented by herbaceous and arbustive-arboreal vegetation covering typically sandy soils [1, 2]. It is characterized by large sandy coastal plains of sedimentary origin that are rippled by rows of dunes isolating lagoons, lakes, ponds, bogs, and marshes. Such a several physical conditions give rise to a diversity of habitats that are colonized by a great variety of vegetal communities [3]. “Restinga” contains many species in common with the Atlantic forest but presents diverse physiological responses to a drier habitat [4].

On this context, “Restinga de Jurubatiba” National Park is an area for permanent preservation of “restinga” habitats (Brazilian sandy coastal plain vegetation) on Rio de Janeiro State, Brazil. This area (22° to 22° 23′ S and 41° 15′ to 41° 45′ W) comprises the municipalities of Macaé, Carapebus and Quissamã [2].

The Myrtaceae family has a great diversity, mainly found across tropical and temperate areas of the globe. It comprises about 4630 species, distributed among about 144 genera [5]. In Brazil, this family is mainly constituted by wood species, being one of the dominant families in Atlantic Forest [6], and it is represented by about 23 genera and 976 species, of which 749 are endemic [7]. Despite the high number of Myrtaceae species found at “restinga” habitats and numerous voucher specimens deposited at herbaria, intricacy related to identification of many species from this family remains [8].

Chemical composition of essential oils from some Myrtaceae species from the “Restinga de Jurubatiba” National Park was evaluated. Meanwhile, essential oil from Eugenia sulcata Spring ex Mart. and Myrciaria floribunda (H.West ex Willd.) O.Berg exhibited anticholinesterase activity [9, 10].

Many species from this family are cultivated due to their edible fruits, source of scents, essences, and as ornamentals, such as Eucalyptus spp. Eugenia uniflora L., Psidium guajava L. and Syzygium jambos (L.) Alston [11]. In addition, numerous species from this family are used in folk medicine, such as Psidium gajava, which is used as antiparasitic, anti-inflammatory, antimicrobial, and treatment of intestinal diseases [12]. Ethnobotanical studies conducted with another species from this family in “restinga” areas indicate several popular uses for them, such as the treatment of diarrhea, sore throat, gout, rheumatism, influenza, urinary tract diseases, diarrhea and diabetes [13]. Species from this family also have great ecological value, since they provide important reward and attract pollinators [14, 15].

The genus Neomitranthes is restricted to Brazil, with sixteen identified species [7]. Neomitranthes obscura (DC.) N. Silveira is an endemic species of the Brazilian Atlantic Forest and widely spread in the sandbanks of “Restinga de Jurubatiba” National Park [16]. It is popularly known by local population as “camboim-de-cachorro” or “cambuí-preto” and commonly used for intestinal disorders as well as food [17, 18].

In the literature, ripe fruits of Neomitranthes obscura are described with black pericarp [8, 19]. However, specimens with yellow ripe fruits were localized during this study in the “Restinga de Jurubatiba” National Park. Both populations occur together, and there are not vegetative distinctions. This species is easily identified by the green cylindrical galls on the apical/axial bud leaf [20] and the globoses fruits crowned by the calyx tube [8]. Fruit color varieties can be found in some Myrtaceae species, but according to Moreno [21], varieties are not easily definable entities in this family and are not widely accepted.

The aim of the present study was to evaluate chemical composition of essential oils obtained from specimens of Neomitranthes obscura (DC.) N. Silveira with different fruit color.

2. Materials and Methods

2.1. Plant Material

Leaves and ripe fruits of Neomitranthes obscura (DC.) N. Silveira were collected from three individuals of each fruit color specimens in “Restinga de Jurubatiba” National Park (Rio de Janeiro, Brazil), in open Clusia scrub vegetation (Black fruits specimens: S 22° 13′ 4.32′′-W 41° 35′ 14.18′′; S 22° 13′ 4.67′′-W 41° 35′ 13.74′′; S 22° 13′ 3.92′′-W 41° 35′ 13.28′′/Yellow fruits specimen: S 22° 13′ 4.43′′-W 41° 35′ 14.83′′; S 22° 13′ 4.00′′-W 41° 35′ 13.96′′; S 22° 13′ 4.04′′-W 41° 35′ 14.17′′). The ripening of the fruits was characterized by the softening, sweetening, and coloring of the tissue (black or yellow pericarp in the different specimens). This species was identified by the botanist Dr. Marcelo Guerra, and voucher of the yellow fruit specimens (L. Rocha 03, 04, 06) and black fruit specimens (L. Rocha 02, 05, 07) were deposited at the herbarium of the Faculdade de Formação de Professores (Universidade do Estado do Rio de Janeiro, Brazil).

2.2. Extraction of the Essential Oils

Fresh leaves from the black fruit specimen (LBF) (2.720 kg), fresh leaves from the yellow fruit specimens (LYF) (2.790 kg), fresh black fruits (BF) (0.896 kg), and fresh yellow fruits (YF) (0.914 kg) were individually ground with distilled water using an automatic blender (Ética Equipamentos Científicos S.A., Brazil). Hydrodistillation method was employed using Clevenger type apparatus, and each plant material was placed in a 5 L flask [9]. The extraction was performed for 4 hours, and after this period, essential oils were collected, dried over anhydrous sodium sulphate, and stored at 4°C for further analyses.

2.3. Gas Chromatography/Mass Spectrometry Analysis

Essential oils were analyzed by a QP2010 (SHIMADZU) gas chromatograph equipped with a mass spectrometer using electron ionization. The gas chromatographic (GC) conditions were as follows: injector temperature, 260°C; detector temperature, 290°C; carrier gas (Helium), flow rate 1 mL/min and split injection with split ratio 1 : 40. Oven temperature was initially 60°C and then raised to 290°C at a rate of 3°C/min. One microliter of each sample, dissolved in CH₂Cl₂ (1 : 100 mg/L), was injected at RTX-5 column (i.d. = 0.25 mm, length 30 m, film thickness = 0.25 m). Mass spectra were recorded at 70 eV with a mass range from m/z 35 to 450 and scan rate of 1 scan/s. The retention indices (AI) were calculated by interpolation of retention times of the substances to the retention times of a mixture of aliphatic hydrocarbons (C7-C40) (Sigma) analyzed in the same conditions [25]. The identification of substances was performed by comparison of their retention indices and mass spectra with those reported in the literature [26]. The MS fragmentation pattern of compounds was also checked with NIST mass spectra libraries. Quantitative analysis of the chemical constituents was performed by flame ionization gas chromatography (GC/FID) with a QP2010 (SHIMADZU) gas chromatograph, under the same conditions of GC/MS analysis and percentages obtained by FID peak-area normalization method.

3. Results and Discussions

According to Souza and Morim [8], the species N. obscura has globoses fruits crowned by the calyx tube with black pericarp when ripe. These characteristics are helpful in the identification of this species [8]. During our study, it was observed not only specimens with the characteristic black ripe fruits, but also populations with ripe yellow fruits (Figure 1). The use of essential oils in studies of intra- and interspecific genetic diversity and geographic patterns of variation in several plant species is well recognized [27]. Thus, an investigation was performed to analyze the chemical pattern of essential oils from these different colorful fruit specimens.

Figure 1: Ripe fruits of specimens from Neomitranthes obscura (DC.) N. Silveira in “Restinga de Jurubatiba” National Park. (a) Black fruits specimen. (b) Yellow fruits specimen.

After extraction, the essential oils obtained from LBF and LYF yielded 0.50% (w/w) and 0.37% (w/w), respectively. Essential oils from BF and YF yielded 0.02% (w/w) and 0.07% (w/w), respectively.

The chemical analysis performed by GC-MS/GC-FID indicated a predominance of sesquiterpenes on both leaves essential oils, corresponding to 81.1% and 84.8% of relative composition of LBF and LYF, respectively. These contents were mainly constituted by sesquiterpenes hydrocarbons. Oxygenated sesquiterpenes and monoterpene hydrocarbons also appeared on leaves oils, while aliphatic compounds appeared just on LBF specimens.

This correlation of major constituents was not observed for each fruits analyzed. BF essential oil showed a predominance of monoterpenes (50.5%) and was constituted by 10.5% of sesquiterpenes. Meanwhile, YF essential oil presented sesquiterpenes (39.9%) as major substances and was constituted by 16.1% of monoterpenes. In all, 27 substances were identified on this essential oil. Both essential oils from fruits presented aliphatic compounds, corresponding to 0.9% and 1.4% of relative composition of BF and YF, respectively.

Germacrene B was the major substance found in LBF essential oil, corresponding to 21.8% of relative composition. The major substances found in the essential oil from LYF were Selina-3,7(11)diene (18.7%), Trans-dauca-4,(11),7-diene (13.9%), and 2,4-di-tert-butylphenol (13.4%), while germacrene B corresponded to 8.4% of relative composition of this essential oil. On another study carried out with leaves from N. obscura, De Ramos et al. [28] showed that sesquiterpenes corresponded to 87% of relative composition of this essential oil, which is in accordance with our results.

-pinene (13.5%) and -pinene (11.0%) were the major substances found in the essential oil from BF, however, appeared in lower amounts in the essential oil from YF (-pinene, 3.2%/-pinene, 1.8%). Caryophyllene oxide, which appeared as the major substance in the essential oil from YF (12.6%), corresponded to 1.7% of relative composition of essential oil from BF. The substances found in the essential oils from leaves and fruits of N. obscura are presented in Table 1.

Table 1: Chemical constituents of essential oil from leaves and fruits of Neomitranthes obscura (DC). N. Silveira.

Plant part

Compound
AI
Leaves
Fruits

Black
Yellow
Black
Yellow

Hexanal
803
—
—
—
0.6

Unidentified¹
812
0.3
1.7
—
—

Unidentified²
854
—
—
1.8
0.8

α-Pinene
936
0.9
1.7
11.0
1.8

Unidentified³
954
—
—
0.9
—

(3Z)-Octen-2-ol
968
—
—
0.9
—

-Pinene
980
1.2
2.0
13.5
3.2

Myrcene
993
0.5
—
1.6
—

-Phellandrene
1008
0.4
—
—
—

-3-Carene
1013
1.0
2.1
—
—

o-Cymene
1027
—
—
0.7
0.6

Limonene
1031
1.3
2.7
2.4
0.7

Unidentified⁴
1106
—
—
—
0.6

-Camphollenal
1129
—
—
1.3
0.6

Trans-pinocarveol
1142
—
—
3.4
1.5

Trans-verbenol
1148
—
—
1.0
0.7

Pinocarvone
1166
—
—
1.0
—

p-Cymen-8-ol
1188
—
—
2.6
2.0

-Terpineol
1194
—
—
4.1
1.7

Myrtenol
1200
—
—
3.0
1.5

Verbenone
1213
—
—
1.2
—

1,3,3-Trimethyl-2-oxabicyclo[2.2.2]octan-6-ol [22]
1226
—
—
1.3
—

Unidentified⁵
1278
—
—
1.2
—

Unidentified⁶
1317
—
—
2.6
—

Trans-p-menth-6-en-2,8-diol
1381
—
—
1.2
—

Sativene
1397
3.1
2.4
—
1.2

Unidentified⁷
1403
—
—
2.4
—

(E)-Caryophyllene
1424
7.0
6.2
—
—

Carvone hydrate
1428
—
—
1.2
—

-Elemene
1437
2.3
—
—
—

-Guaiene
1443
1.5
—
—
—

-Humulene
1458
0.6
—
—
—

-Gurjunene
1481
1.0
—
—
—

Unidentified⁸
1485
—
—
—
0.9

β-Selinene
1491
6.3
6.0
1.6
2.1

Unidentified⁹
1496
1.9
—
—
—

Unidentified¹⁰
1498
—
—
1.9
—

-Selinene
1500
6.4
5.7
—
—

-Bulnesene
1510
1.1
—
—
—

-Bisabolene
1512
—
3.4
—
—

2,4-di-tert-butylphenol [23]
1516
1.9
13.4
—
—

7-epi--Selinene
1523
2.5
2.3
—
—

(E)--Bisabolene
1536
—
4.4
—
—

Selina-3,7,(11)diene
1540
14.1
18.7
—
—

Unidentified¹¹
1543
4.7
5.0
—
—

Trans-dauca-4 (11)7-diene
1547
11.4
13.9
—
—

Unidentified¹²
1558
—
—
—
0.8

Germacrene B
1563
21.8
8.4
—
—

Unidentified¹³
1568
0.8
—
—
—

Caryophyllene oxide
1589
—
—
1.7
12.6

Unidentified¹⁴
1594
—
—
—
2.5

Humulene epoxide II
1615
—
—
—
2.2

Unidentified¹⁵
1619
—
—
—
1.5

Unidentifiedl6
1624
0.9
—
—
—

Unidentified¹⁷
1633
—
—
1.9
2.6

Unidentified¹⁸
1638
—
—
—
1.3

Desmetoxy encecalin
1650
—
—
2.3
3.6

β-Eudesmol
1656
—
—
—
1.0

Unidentified¹⁹
1658
0.6
—
—
0.6

Unidentified²⁰
1659
0.2
—
—
—

Selin-11-en-4α-ol
1661
1.2
—
3.5
6.0

Allohimachalol
1663
—
—
1.4
—

Unidentified²¹
1671
0.7
—
—
—

-(z)-Santalol
1677
—
—
—
2.0

Cadalene
1681
—
—
—
1.2

Unidentified²²
1687
—
—
—
0.9

Unidentified²³
1689
—
—
—
1.1

Unidentified²⁴
1693
—
—
1.3
1.2

Juniper camphor [24]
1702
—
—
—
1.1

Unidentified²⁵
1723
—
—
—
1.3

Unidentified²⁶
1733
—
—
—
1.5

Cyclocolorenone
1757
—
—
—
1.9

Unidentified²⁷
1764
—
—
3.5
1.1

Aristolone
1768
—
—
—
2.1

Unidentified²⁸
1778
—
—
—
1.7

Unidentified²⁹
1791
—
—
4.1
4.5

Unidentified³⁰
1796
—
—
—
0.7

Unidentified³¹
1801
—
—
—
0.9

Unidentified³²
1804
—
—
—
1.2

Unidentified³³
1808
—
—
—
1.2

Unidentified³⁴
1814
—
—
1.98
3.1

Unidentified³⁵
1826
—
—
—
2.4

Unidentified³⁶
1872
0.8
—
—
—

Unidentified³⁷
1875
—
—
—
1.2

Unidentified³⁸
1879
—
—
2.0
—

Unidentified³⁹
1906
—
—
1.2
2.0

Unidentified⁴⁰
1910
—
—
—
1.1

Unidentified⁴¹
1924
0.8
—
2.4
—

Unidentified⁴²
1952
—
—
4.1
—

Unidentified⁴³
1955
—
—
—
5.1

Unidentified⁴⁴
1963
—
—
2.1
1.6

Unidentified⁴⁵
1967
—
—
2.9
1.9

Unidentified⁴⁶
2034
—
—
—
1.0

Unidentified⁴⁷
2347
0.7
—
—
—

Total identified

86.1
93.3
70.0
70.2

MS m/z (Relat. int.): 40; 41; 45 (100); 69; 71; 87.

2MS m/z (Relat. int.): 41; 43 (100); 69; 83; 97; 98.

3MS m/z (Relat. int.): 55 (100); 69; 70; 97; 98; 112.

4MS m/z (Relat. int.): 41; 43; 57 (100); 70; 96; 98; 114; 207.

5MS m/z (Relat. int.): 41; 43; 69; 83 (100); 97; 125; 135; 207.

6MS m/z (Relat. int.): 41; 43 (100); 59; 71; 84; 100; 123.

7MS m/z (Relat. int.): 41; 43 (100); 59; 71; 95; 109; 123; 133; 151; 166.

8MS m/z (Relat. int.): 41; 55; 67; 79; 93; 107; 121; 135; 145; 163; 178 (100); 2004.

9MS m/z (Relat. int.): 41; 57; 71; 91; 105; 119; 133; 147; 161 (100); 175; 189; 204.

10MS m/z (Relat. int.): 41; 43; 59; 71; 97; 108; 112; 126; 155; 189; 204.

11MS m/z (Relat. int.): 41; 55; 67; 81; 91; 105; 119; 133; 147; 161; 175; 189 (100); 204.

12MS m/z (Relat. int.): 41; 69; 79 (100); 91; 106; 121; 135; 149; 159; 177; 187; 205; 220.

13MS m/z (Relat. int.): 41; 55; 69; 79; 91; 75; 119; 133; 148; 161; 189 (100); 204.

14MS m/z (Relat. int.): 41; 55; 67; 79; 91; 108; 119; 135; 147; 163; 178 (100); 192; 202; 220.

15MS m/z (Relat. int.): 41; 43 (100); 67; 81; 95; 109; 123; 135; 147; 161; 178; 189; 204; 222.

l6MS m/z (Relat. int.): 41; 43; 67; 81; 93; 105; 123 (100); 135; 147; 161; 189; 204.

17MS m/z (Relat. int.): 40; 44; 69; 81; 91; 105; 120; 131; 145; 159; 173; 187; 202 (100).

18MS m/z (Relat. int.): 41; 43; 59; 79; 91; 105; 119; 135; 145 (100); 161; 177; 185; 204; 218.

19MS m/z (Relat. int.): 41; 69; 81; 95; 100; 125 (100); 135; 147; 164; 177; 187; 205; 220.

20MS m/z (Relat. int.): 44; 55; 81; 93; 107; 133 (100); 149; 161; 173; 187; 202.

21MS m/z (Relat. int.): 41; 43; 67; 81; 93; 105; 122; 133; 148; 161; 189 (100); 104.

22MS m/z (Relat. int.): 41; 43; 69 (100); 79; 93; 109; 123; 133; 151; 160; 175; 187; 207; 220; 236.

23MS m/z (Relat. int.): 41; 55; 67; 81; 96 (100); 107; 123; 126; 149; 165; 179; 194; 205; 222; 252.

24MS m/z (Relat. int.): 41; 43 (100); 69; 71; 93; 109; 121; 153; 167; 178; 196.

25MS m/z (Relat. int.): 41; 43; 67; 79; 91; 105; 123; 138; 151 (100); 161; 179; 194; 205; 218.

26MS m/z (Relat. int.): 41; 43 (100); 67; 83; 93; 111; 123; 135; 149; 161; 175; 193; 208; 221; 236.

27MS m/z (Relat. int.): 41; 43; 60 (100); 73; 85; 99; 115; 129; 143; 157; 171; 185; 199; 228.

28MS m/z (Relat. int.): 41; 43 (100); 67; 79; 93; 108; 119; 138; 145; 161; 176; 194; 208; 218; 231.

29MS m/z (Relat. int.): 41; 59; 81; 95; 105; 119; 133; 147; 161; 179 (100); 187; 205; 220.

30MS m/z (Relat. int.): 41; 55; 67; 79; 96 (100); 107; 121; 135; 149; 162; 176; 189; 208; 218; 234.

31MS m/z (Relat. int.): 41; 43 (100); 59; 79; 91; 107; 123; 135; 147; 160; 177; 187; 205; 223; 232; 238.

32MS m/z (Relat. int.): 41; 67; 79; 93 (100); 108; 119; 133; 151; 161; 179; 187; 203; 221; 238.

33MS m/z (Relat. int.): 41; 43; 69; 81; 91; 107; 123 (100); 135; 145; 159; 177; 187; 205; 220; 236.

34MS m/z (Relat. int.): 41; 43; 69; 81; 95; 109; 123 (100); 135; 141; 162; 177; 191; 205; 220; 238.

35MS m/z (Relat. int.): 41; 43; 67; 83; 97; 105; 121 (100); 133; 145; 161; 176; 194; 196; 218; 236.

36MS m/z (Relat. int.): 40; 57; 76; 93; 104; 149 (100); 167; 207; 223.

37MS m/z (Relat. int.): 43 (100); 59; 71; 95; 99; 119; 137; 151; 159; 177; 193; 199; 219; 234; 252.

38MS m/z (Relat. int.): 41; 69; 81; 95; 105; 123; 135; 150; 164 (100); 179; 205; 220; 238.

39MS m/z (Relat. int.): 43; 69; 81; 97; 109; 121; 135; 150; 161; 179 (100); 197.

40MS m/z (Relat. int.): 41; 69; 79; 95; 107; 123; 138 (100); 149; 159; 173; 191; 201; 216; 234.

41MS m/z (Relat. int.): 41; 55; 57 (100); 71; 85; 99; 113; 127; 141; 155; 175; 189; 205; 217; 232; 261.

42MS m/z (Relat. int.): 41; 43; 59; 81; 97; 109 (100); 121; 135; 147; 161; 179; 191; 197; 219; 234.

43MS m/z (Relat. int.): 41; 43; 59; 81; 97; 109 (100); 121; 135; 147; 161; 179; 191; 197; 219; 233; 238.

44MS m/z (Relat. int.): 41; 43 (100); 60; 73; 87; 101; 115; 129; 143; 157; 174; 185; 199; 213; 256.

45MS m/z (Relat. int.): 41; 43; 69; 81; 97; 109; 121; 135; 149; 161; 179; 187; 197 (100); 220; 238.

46MS m/z (Relat. int.): 43; 67; 81; 93; 105; 119; 133; 149; 159; 177; 195 (100); 217.

47MS m/z (Relat. int.): 41; 55; 69; 85; 99; 113; 127; 165; 179 (100); 207; 261; 343.

In the tropical and subtropical zones, fruits mainly consumed by mammals are often yellow or orange, while fruits consumed by birds are often red or black [29, 30]. The occurrence of two different colors for a fruit species may increase its dispersal. On another study, Gomes et al. [4] concluded that availability peak of caloric fruits coincides with energy-demanding seasons for resident and nonbreeding birds in “Restinga de Jurubatiba” National Park, despite the fact that there was no mention of different fruit colors. In addition, regarding the literature data, we can observe that essential oil from N. obscura was investigated but without any mention of differences in fruit color [28].

4. Conclusions

Varieties are not easily definable entities in the Myrtaceae family; however, a study carried out for different coloring fruits of Eugenia brasiliensis Lam. indicated that analysis of its volatiles corroborated the concept of different varieties for this species [21]. To our knowledge, this is the first report about different fruit colors of N. obscura. It is interesting from a chemical overview that the content of predominant volatiles from the two analyzed fruits had an inverse relation between sesquiterpenes and monoterpenes. These results suggest that preferable metabolic production of monoterpenes or sesquiterpenes is followed for each specimen. Thus, the color and chemical constituents of different fruits of N. obscura suggest that this Myrtaceae species contains at least two different varieties.

Acknowledgment

The authors would like to thank Fundação de Amparo a Pesquisa do Estado do Amapá (Fundação Tumucumaque) for their finantial support through the Programme franco-brésilien de coopération scientifique et universitaire-AIRD-FAP Tumucumaque and Dr. Marcelo de Costa Souza for assistance in the certification of the identified Myrtaceae species.

References

D. S. D. Araújo, “Vegetation types of sandy coastal plains of tropical Brazil: a first approximation,” in Coastal Plant Communities of Latin America, U. Seeliger, Ed., p. 392, Academic press, New York, NY, USA, 1992.

L. Cogliatti-carvalho, A. F. N. Freitas, C. F. D. Rocha, and M. V. Luys, “Variação na estrutura e na composição de Bromeliaceae em cinco zonas de restinga no Parque Nacional da Restinga de Jurubatiba, Macaé, RJ,” Revista Brasileira De Botânica, vol. 24, no. 1, pp. 1–9, 2001.

A. Kelecom, G. L. Reis, P. C. A. Fevereiro et al., “A multidisciplinary approach to the study of the fluminense vegetation,” Anais da Academia Brasileira de Ciencias, vol. 74, no. 1, pp. 171–181, 2002.

V. S. D. M. Gomes, M. S. Buckeridge, C. O. Silva, F. R. Scarano, D. S. D. Araujo, and M. A. S. Alves, “Availability peak of caloric fruits coincides with energy-demanding seasons for resident and non-breeding birds in restinga, an ecosystem related to the Atlantic forest, Brazil,” Flora, vol. 205, no. 10, pp. 647–655, 2010.

W. S. Judd, C. S. Campell, E. A. Kellogg, P. F. Stevens, and M. J. Donoghue, Sistemática Vegetal: Um Enfoque Filogenético, Artmed Editora, Porto Alegre, Brazil, 3rd edition, 2009.

F. F. Mazine and V. C. Souza, “Myrtaceae dos campos de altitude do Parque Nacional do Caparaó—Espírito Santo/Minas Gerais, Brasil,” Rodriguésia, vol. 59, no. 1, pp. 57–74, 2008.

M. Sobral, C. Proença, M. Souza, F. Mazine, and E. Lucas, “Myrtaceae,” in Lista De Espécies Da Flora Do Brasil, Jardim Botânico do Rio de Janeiro, 2012.

M. D. C. Souza and M. P. Morim, “Subtribes eugeniinae O. Berg and Myrtinae O. Berg (Myrtaceae) at marambaia restinga, Rio de Janeiro State, Brazil,” Acta Botanica Brasilica, vol. 22, no. 3, pp. 652–683, 2008.

B. G. Lima, L. A. C. Tietbohl, C. P. Fernandes et al., “Chemical composition of essential oils and anticholinesterasic activity of Eugenia sulcata spring ex mart,” Latin American Journal of Pharmacy, vol. 31, no. 1, pp. 152–155, 2012.

L. A. C. Tietbohl, B. G. Lima, C. P. Fernandes et al., “Comparative study and anticholinesterasic evaluation of essential oils from leaves, steams and flowers of Myrciaria floribunda(H. West ex. Willd.) O. Berg,” Latin American Journal of Pharmacy, vol. 31, no. 4, pp. 637–641, 2012.

E. Gressler, M. A. Pizo, and P. C. Morellato, “Polinização e dispersão de sementes de Myrtaceae do Brasil,” Revista Brasileira de Botanica, vol. 29, pp. 509–530, 2006.

M. P. Gupta, 270 Plantas Medicinales Iberoamericanas, CYTED-SECAB, Santafé de Bogotá, Colombia, 1995.

O. H. Boscolo and L. Senna-Valle, “Plantas de uso medicinal em Quissamã, Rio de Janeiro, Brasil,” Iheringia, vol. 63, no. 2, pp. 263–277, 2008.

C. Proença, The reproductive biology and taxonomy of the Myrtaceae of the Distrito Federal (Brazil) [Ph.D. thesis], University of St. Andrews, 1991.

L. R. Landrun and D. Stevenson, “Variability of embryos in the subtribe Myrtinae (Myrtaceae),” Systematic Botany, vol. 11, pp. 155–162, 1996.

M. Sobral, A Família Das Myrtaceae No Rio Grande Do Sul, Editora Unisinos, 2003.

M. G. Santos, P. C. A. Fevereiro, G. L. Reis, and J. I. Barcelos, “Recursos vegetais da Restinga de Carapebus,” Revista de Biologia Neotropical, vol. 6, pp. 35–54, 2009.

L. R. Zamith and F. R. Scarano, “Produção de mudas de espécies das restingas do município do Rio de Janeiro,” Acta Botanica Brasilica, vol. 18, no. 1, pp. 161–176, 2004.

M. C. Souza, Estudos taxonômicos em Myrtaceae no Brasil: Revisão de Neomitranthes Kausel ex D.Legrand e contribuição ao conhecimento da diversidade e conservação de Plinia L. no Domínio Atlântico [Ph.D. thesis], Instituto de Pesquisas Jardim Botânico do Rio de Janeiro, Escola Nacional de Botânica Tropical, Rio de Janeiro, Brazil, 2009.

V. C. Maia, “Chaves para classificação de galhas de Cecidomyiidae (Diptera) em Myrtaceae na restinga da Barra de Maricá, Rio de Janeiro,” Revista Brasileira De Zoologia, vol. 12, pp. 1009–1013, 1995.

P. R. H. Moreno, M. E. L. Lima, M. Sobral et al., “Essential oil composition of fruit colour varieties of Eugenia brasiliensis Lam,” Scientia Agricola, vol. 64, no. 4, pp. 428–432, 2007.

B. Tirillini, G. Verdelli, F. Paolocci, P. Ciccioli, and M. Frattoni, “The volatile organic compounds from the mycelium of Tuber borchii vitt,” Phytochemistry, vol. 55, no. 8, pp. 983–985, 2000.

S. Hamm, J. Bleton, and A. Tchapla, “Headspace solid phase microextraction for screening for the presence of resins in Egyptian archaeological samples,” Journal of Separation Science, vol. 27, no. 3, pp. 235–243, 2004.

R. P. Adams, Identification of Eessential Oil Components By Gas Chromatography/Mass Spectrometry, Allured Publishing, Carol Stream, 1995.

H. van Den Dool and P. D. Kratz, “A generalization of the retention index system including linear temperature programmed gas-liquid partition chromatography,” Journal of Chromatography A, vol. 11, pp. 463–471, 1963.

R. P. Adams, Identification of Essential Oil Components by Gas Chromatography/Mass Spectrometry, Allured Publishing, Carol Stream, 4th edition, 2007.

C. Messaoud, A. Béjaoui, and M. Boussaid, “Fruit color, chemical and genetic diversity and structure of Myrtus communis L. var. italica Mill. morph populations,” Biochemical Systematics and Ecology, vol. 39, pp. 570–580, 2011.

M. F. S. De Ramos, S. S. Da Monteiro, V. P. Da Silva, M. J. Nakamura, and A. C. Siani, “Essential oils from myrtaceae species of the Brazilian Southeastern maritime forest (Restinga),” Journal of Essential Oil Research, vol. 22, no. 2, pp. 109–113, 2010.

M. F. Willson and C. J. Whelan, “The evolution of fruit color in fleshy-fruited plants,” The American Naturalist, vol. 136, no. 6, pp. 790–809, 1990.

M. F. Willson, D. A. Graff, and C. J. Whelan, “Color preferences of frugivorous birds in relation to the colors of fleshy fruits,” The Condor, vol. 92, pp. 545–555, 1990.