Ethnobotanical Research at the Kutukú Scientific Station, Morona-Santiago, Ecuador

This work features the results of an ethnobotanical study on the uses of medicinal plants by the inhabitants of the region near to the Kutukú Scientific Station of Universidad Politécnica Salesiana, located in the Morona-Santiago province, southeast of Ecuador. In the surroundings of the station, one ethnic group, the Shuar, has been identified. The survey hereafter reports a total of 131 plant species, with 73 different therapeutic uses.


Introduction
Plants have played a fundamental role for the development of Andean cultures ever since man first arrived to that region approximately 10000 years ago [1]. Through history, man has utilized vegetable resources as a source of nutrition, medicines, fuel, and building materials and they even occupied an important place within their belief system and rites [2].
According to the World Health Organization [3,4] about 80% of the world population uses natural remedies and traditional medicine. Such medicine comes from forest resources that provide a series of benefits to the local, regional, and national populations and include raw materials, patrimony protection, and scenic beauty [5]. Nowadays, Ecuador, located on the equator line, is considered as one of the countries with the greatest biodiversity in the world [6]. In Ecuador, there exist many areas of biological interest that for their unique features currently provide diverse uses for the benefit of the population [7]. These uses of natural resources are the fruits of the accumulation of the ancestral knowledge that the local population has kept until now [8]. This traditional knowledge has been orally transmitted from one generation to the next, but over the last decades, the crisis of the rural world threatens this rich patrimony, which may be lost, together with many interesting aspects concerning the traditional exploitation of natural resources [9].
In Ecuador, many ethnobotanical studies have been made: that is, Villacrés [10] studied the bioactivity of plants of the Amazon jungle from the province of Napo; Cerón and Montalvo [11] published a book on the ethnobotanical aspects of the "Huaorani" people in a specific zone in the northeast of Ecuador; the cultural practices of Quechua society in Napo were analyzed in a book by Iglesias [12]; Tene et al. [13] performed an ethnobotanical study on medicinal plants used in Loja and Zamora Chinchipe. A few studies have also been made concerning the traditional use of plants in the Saraguro community of south Ecuador [14][15][16].
In this work we analyzed the ethnobotanical patrimony of Kutukú Scientific Station, located on the Kutukú mountain range in the Morona-Santiago province, Ecuador. By doing that, we intended to safeguard the popular knowledge concerning plants and to produce a database of plant uses and advantages. This data could be used by the citizens themselves and could be the base for future actions in programs of scientific investigations, environmental education, social

Methodology
The research herein was performed between August and December of 2013, within the area of the Kutukú Biological Station belonging to Universidad Politécnica Salesiana del Ecuador (2 ∘ 18 39.1 S, 78 ∘ 06 11.6 W) with 800-1200 m a.s.l. The territory of the station covers 250 hectares and is located within the Kutukú-Shaimi Protected Forest (BPKS) in the center of Ecuador's eastern region, east of the city of Macas, in the parish of Sevilla Don Bosco, province of Morona-Santiago ( Figure 1). Within the area of the scientific station, big forest extensions of the evergreen humid type, really difficult to access, still persist in the low montane, montane, and high montane belts [17]. The month average temperature ranges between 16 and 27 ∘ C, and the total annual precipitation is 3021 mm approximately [18]. Close to the territory of the scientific station, where this study was carried out, about 7 indigenous communities can be found; all of these belong to the ethnic group "Shuar" (Table 1).
For this investigation, a qualitative ethnobotanical method was carried out [20] in which mostly adult persons have been contacted, who live within the area under study and know the use of medicinal plants. One hundred and sixty inhabitants of the seven communities were interviewed. All respondents were farmers or elderly belonging to the "Shuar" ethnicity or their descendants.
The collected data were processed and the results are expressed in a list of medicinal plants, with scientific names, families, collection point, used parts, form of preparation, traditional uses, and distribution ( Table 2). The plant samples were processed and identified at the Ecuadorian National Herbarium (QCNE). They were deposited with their respective code at the Herbarium of the Universidad Politécnica Salesiana in the city of Quito, Ecuador. The botanic nomenclature was registered according to the Catalog of Vascular Plants of Ecuador [6]. A contact was established with the Shuar community through their authorized representatives, with the possibility of performing more precise studies in the future for the recovery, protection, and sustainable use of their traditional knowledge.

Result and Discussion
The ethnobotanical study performed in this work gave us a real panorama about the natural remedies used by the inhabitants in the territory of the Kutukú Scientific Station of the Morona canton of the province of Morona-Santiago, south of Ecuador. This research was realized with "Shuar" community, which is very different from the "Achuar" community cited in the article by Giovannini [21], even the geographical location and the altitude are different.
One hundred thirty-one different plants have been identified, which many medicinal properties have been attributed to; their uses and forms of therapeutic usage also have been recorded. From the total number of plants, 107 are native, 9 are grown traditionally, 9 are endemic, and 6 are cultivated [6].              This long list of plants comprises species yet unknown from a phytochemical point of view, as well as deeply studied ones, for example, Croton lechleri Müll. Arg. and Ilex guayusa Loes. Croton lechleri is used locally as a wound healing aid, as a treatment for ulcers and sometimes for vaginal infections. These usages and other are scientifically validated in literature, especially concerning the treatment of infections and the wound healing aid [22]. According to another author [13], Croton lechleri is traditionally used in Ecuador as a diuretic remedy and is also employed to treat dermatologic and hepatic illnesses.
Ilex guayusa is used in case of gastritis, as a stimulant replacing coffee, and to enhance fertility in women. In the Amazon forest of Ecuador and Peru, a traditional guayusa decoction that yields a high content of caffeine is used as a stimulant in the morning [23]. It is also reported [24] that Ilex guayusa of Peru has a strong antimicrobial activity.
Verbena litoralis Kunth keeps being very appreciated in local medicine, particularly for the treatment of headaches, ulcers, and alopecia. Data in scientific literature [25] support its activity as an analgesic too.
Virola pavonis is used and very appreciated by the local inhabitants for the treatment of diseases, from inflammation to headache, from hangovers to renal problems. Outside Ecuador, this plant was tested in Brazil for the treatment of leishmaniosis [26], while the antifungal activity of the extracts from the leaves was studied in detail by Zacchino et al. [27]. The leaves of Siparuna harlingii S. S. Renner & Hausner are used by the local inhabitants as remedies for headache and cough and as an anti-inflammatory. At other latitudes, different authors described diverse applications of this plant: in Zamora Chinchipe province of Ecuador, the inhabitants employ it against rheumatism [28]; meanwhile in Colombia, it is used to treat illnesses of nervous system and stomach [29].
The bark of Byrsonima arthropoda in decoction is employed as an analgesic, although the whole plant is used as food for birds and mammals. The Makuna Indians from Praparaná River, a Colombian Amazon ethnic group, uses the ground plant as a wound healing aid [30].
In our study we found that the leaves of Acmella ciliata are used as a remedy against diarrhea, headaches, colds, and toothache and for "mal aire." Only a few papers are available in literature, either ethnopharmacologic or phytochemical, for Acmella ciliata. In a study performed in the state of Minas Gerais in Brazil [31] it is reported that the leaves of this plant are traditionally used to treat hepatic problems.
From the flowers and leaves of Citharexylum poeppigii aqueous infusions are prepared, which are used to treat affections of upper respiratory tract such as cough. This infusion is also used for digestive discomfort, headaches, and menstrual cramps. In literature information concerning the ethnobotanical use of this species is lacking; nonetheless, the presence of this plant is confirmed in Ecuador [32] and Venezuela [33].
In the area of Kutukú Scientific Station plants of the Rubiaceae family are used mostly as anticarcinogens (4), for circulatory system problems (4), as a treatment for diabetes (3), and against malaria (2). In literature plants of the Rubiaceae family are reported as being antihepatotoxic [34], as well as having anti-inflammatory and analgesic activities [35].
Piperaceae plants are often used locally against kidney and urinary tract affections (3), as wound healing aids (3), and curiously sometimes as an abortive (2). The use of the plants of this family in infusions of whole plant is frequently reported in the literature, which confirms the usage as wound healing aid [36] and for the treatment of kidney affections [37].
In this study we found that the plants of the Melastomataceae family are used especially as treatment of poisonous snake bites and for wound healing (4). Many plants of this family are used in Mexico for the traditional treatment of gingivitis and oral infections overall [38] and have been tested in Brazil for treating gastrointestinal problems [39].
We confirm the usage of plants of the Malvaceae family as diuretic remedies, as reported, for example, in Alarcón-Alonso et al. [40] for Hibiscus sabdariffa L., and also the use of Patinoa sphaerocarpa Cuatrec in the same way. The antiviral properties of the plants of the Arecaceae family, reported, for example, in Cocos nucifera L. from Brazil [41], are confirmed  (2), kidney problems (2), and infections of the urinary tract (2). A study carried out in the Bolivian Chaco pointed out the traditional use of some species of Solanaceae family for the treatment of headaches, fever, acne, and diarrhea [42]; these pharmacological uses can be correlated with our findings. Besides, the Solanaceae family has been broadly studied and its plants are used for their hallucinogenic properties [43].
Plants of the Euphorbiaceae family are used mostly as wound healing aids (2), for intestinal problems (1), for diarrhea (1), and as hepatoprotective agents (1), the latter being confirmed by a study performed in India [44].
Many plants of these families and of the other families reported in this study are used for the treatment of "espanto" or "mal aire," a typical Andean pathology that is poorly defined, in which the psychosomatic problems, originated from phobia factors, produce deep physical and psychological weakness [45]. To talk about etiology of "mal aire" is not just talk about the origin of the disease but rather is to speak of the limits of medicine and get into the field of philosophical approaches; in general the "mal aire" is a state of general decay of the body generated by evil spirits that inhabit the crossing mountains and under large trees, orchards abandoned, ponds, streams, cemeteries, places where they have been burials fact of valuables, and so forth [46].
An aqueous infusion is the most generalized form of administration; decoctions, direct consumption, and topical applications are also common. The leaves (50%) are the plant parts more frequently selected, followed by the stem (12%), fruits (16%), and barks (10%).

Conclusions
The use of plants for treating diseases keeps being necessary to indigenous populations in emerging countries which, due to high costs, have a difficult access to western medicines and, therefore, use to rely on their own traditional remedies. On the other hand, the integrated forms of modern and traditional medicines are often practiced by many physicians nowadays. Morona-Santiago, the Ecuadorian province where the Kutukú Scientific Station is located, owns a high plant biodiversity and a rich ethnobotanical tradition based on it. This has given origin to a popular medicine comprising the use of more than 100 local plants, which have been botanically identified. The illnesses cured with the plants are, of course, strictly related with the health situation of local communities. Wound healing aids, renal and stomach infections, headaches, and colds are the most common ailments which natural resources are used for. Nonetheless less frequent diseases, such as cancer, diabetes, and malaria, are also treated with different plant extracts.
On these days, the preservation of traditional knowledge is threatened by a great amount of external factors related to the "modernization" of the region. It is, therefore, urgent to save the cultural patrimony of the indigenous populations, by confirming the therapeutic use of plants with scientific criteria and by encouraging the phytochemical research of the species containing potential active principles of interest.
Within this context, more studies on the use of medicinal plants by the indigenous communities nearby the Kutukú Scientific Station are being carried out by our investigation groups, in which scientists, technicians, and students are involved, assessing the biological activity of the region's most promising plants.