Overexpression of CaAPX Induces Orchestrated Reactive Oxygen Scavenging and Enhances Cold and Heat Tolerances in Tobacco

Ascorbate peroxidase (APX) acts indispensably in synthesizing L-ascorbate (AsA) which is pivotal to plant stress tolerance by detoxifying reactive oxygen species (ROS). Enhanced activity of APX has been shown to be a key step for genetic engineering of improving plant tolerance. However it needs a deeper understanding on the maintenance of cellular ROS homeostasis in response to stress. In this study, we identified and characterized an APX (CaAPX) gene from Camellia azalea. Quantitative real-time PCR (qRT-PCR) analysis showed that CaAPX was expressed in all tissues and peaked in immature green fruits; the expression levels were significantly upregulated upon cold and hot stresses. Transgenic plants displayed marked enhancements of tolerance under both cold and heat treatments, and plant growth was correlated with CaAPX expression levels. Furthermore, we monitored the activities of several ROS-scavenging enzymes including Cu/Zn-SOD, CAT, DHAR, and MDHAR, and we showed that stress tolerance was synchronized with elevated activities of ROS-scavenging. Moreover, gene expression analysis of ROS-scavenging enzymes revealed a role of CaAPX to orchestrate ROS signaling in response to temperature stresses. Overall, this study presents a comprehensive characterization of cellular response related to CaAPX expression and provides insights to breed crops with high temperature tolerances.


Introduction
Temperature stress, such as heat, cold, or freezing, causes an increase in reactive oxygen species (ROS) levels and induces the oxidative stress in plant cells [1]. The elevation of reactive ROS levels can cause deleterious damage to organelle function and cellular metabolism or leads to programed cell death [2]. To quickly remove excessive ROS, plants evolved a complex system containing nonenzymatic antioxidants, such as ascorbate (AsA), glutathione (GSH), flavonoids and carotenoids, and ROS-scanvenging enzymes including superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and enzymes in the water-water cycle [3]. The antioxidants and enzymes need to act synergistically to keep the homeostasis of cellular redox state. For example, the water-water cycle, also known as Halliwell-Asada or AsA-GSH cycle, includes the activity of APX, DHAR, GR, and MDHAR and was a pathway involved in scavenging superoxide radicals and H 2 O 2 [4].
There is an effective regulatory network containing multiple pathways in plant cell to deal with cellular ROS efficiently. The regulation of antioxidants and enzymatic activities was involved in a signaling transduction leading to changes of gene expression levels. Recently the ROS were revealed as central signaling components to orchestrate the multiple scavenging genes expressions under various stressed conditions [5,6]. Upon temperature stress, the cellular ROS levels were upregulated through the "temperature sensor" which amplified the signal to activate the ROS-scavenging enzymes [2,7]. Heat shock proteins (HSPs) and heat shock transcription factors (HSFs) were thought to be responsible for temperature stress sensing [8,9]. On the other hand, the NADPH oxidases (respiratory burst oxidase homologous, RBOHs) played a key role in generating ROS upon stress stimuli, which were involved in different signaling pathways [10,11]. In Arabidopsis, functional analyses of HSF3 and HSF21 revealed that ROS-scavenging enzymes APX1 and APX2 were imminent targets which were required to protect against heat induced oxidative damage [12,13]. In addition, APX could act interactively with RBOHD to generate systematic signals of ROS tolerance [10,13].
Ascorbate peroxidase (APX) was found to be a key enzyme in the ascorbate-glutathione pathway to scavenge cellular H 2 O 2 produced in various stressful conditions [5,[14][15][16][17][18][19]. When APX reduces hydrogen peroxide to water by utilizing AsA as an electron donor, monodehydroascorbate (MDA) accumulates. Meanwhile MDA is somewhat unstable and likely to be rereduced disproportionately into AsA and dehydroascorbate (DHA), and both MDA and DHA can be reduced back to AsA through the activity of reductases [20][21][22]. Many studies over the years have demonstrated that APX played a specific role in improving plant's tolerance response to certain abiotic stresses [19,[23][24][25]. Enhanced expression of APX in transgenic plants could intensify their resistance to multiple environmental stresses through eliminating H 2 O 2 [19,[23][24][25][26][27]. For example, APX overexpression improved chilling tolerances in rice and sweet potato [28,29]; the transgenic potato plants with APX expression were more tolerant to high temperature stress [30,31]. In addition, after exposure to some environmental stresses, the APX knockdown plants exhibited much more severe cellular injuries [32][33][34].
Although the genetic manipulation of ROS has been practiced in many plant species, our understanding of ROS removing and signaling remains to be expanded to better improve the plant performance by the genetic manipulation approach. Here, in order to investigate the potential improvements of temperature stresses in Camellia species, we cloned APX gene from Camellia azalea and investigated its expression pattern. We showed that CaAPX was expressed in all examined Camellia tissues and was quickly induced by the temperature stresses. Functional analysis in transgenic tobacco proved that overexpression of CaAPX enhanced plant performances in both cold and heat conditions. Furthermore, we demonstrated that overexpression of CaAPX altered the cellular content of AsA, MDA, and H 2 O 2 concentrations and led to lower electrolyte leakage rate. Enzymatic activity analysis of APX, SOD, POD, and CAT and gene expression analysis of tobacco endogenous Cu/Zn-SOD, CAT, DHAR, and MDHAR genes revealed that CaAPX may have a central role in synchronizing cellular ROS-scavenging network. This work may provide useful information for engineering plants of high resistance to oxidative damage under temperature stresses.

Plant Materials and
Treatments. C. azalea materials selected for present study including four-year-old grafted seedlings and two-year-old cutting seedlings were collected from a greenhouse of the Research Institute of Subtropical Forestry located in Fuyang (119 ∘ 57 E, 30 ∘ 04 N; Fuyang city, Zhejiang, China), growing under natural light condition with regular irrigation. And subculture aseptic tobacco seedlings with six true leaves (Nicotiana tabacum L., cv. SR1) were grown in a growth chamber at 25 ∘ C and 1200 Lux light (16 h light/8 h dark). For treatments, plants were exposed to 4 ∘ C low and 38 ∘ C high temperatures for 0, 2, 4, 8, and 12 h, respectively, in the growth chamber at 25 ∘ C and 1200 Lux light (16 h day/8 h night). The samples treated for 0 h were set as controls. Plant tissues of C. azalea seedlings and the forth leaves of treatment tobacco plants collected were immediately put into liquid nitrogen and stored at -80 ∘ C for later use.

Cloning of CaAPX Gene.
Total RNA was extracted using the plant total RNA isolation Kit (Aidlab Biotech, China) for reverse transcription polymerase chain reaction (RT-PCR) (Takara, China). To isolate the APX gene, the 3 ,5 -RACE amplification Kit (Takara, China) was used to construct libraries from C. azalea young leaves. Two amplified fragments (gene-specific primers were listed in Table S1 (see Table S1 in Supplementary Material available online at https://doi.org/10.1155/2017/4049534)) were cloned into pGEM-Teasy vector (Promega, USA) for sequencing, and full-length cDNA sequence was assembled. The cDNA sequence was designated as CaAPX and submitted to Gen-Bank (accession number KP635267) (https://www.ncbi.nlm .nih.gov/genbank/).

Quantitative
Real-Time PCR. DNA traces of the total RNA were removed by DNase I (RNase-free) (TransGen Biotech, China). The cDNAs from different organs of C. azalea cutting seedlings on, respectively, different temperature stress levels were obtained by employing the PrimeScript6 RT reagent Kit (Perfect Real Time) (Takara, China). The 18S RNA gene of Camellias was used as an internal control to normalize the amount of mRNA present in each sample. And all samples were amplified in four independent replicates by an Applied Biosystems 7300 Fast Real-Time PCR system using SYBR-Premix Ex Taq6 II (Tli RNadeH Plus) (Takara, China) for fluorescence detection and the expression data was analyzed using 2 −ΔΔCt method.

Construction of the Plant Expression Vector and Transformation of Tobacco.
Full-length cDNA sequence of CaAPX gene was obtained by amplification of primers containing XbaI and BamHI restriction sites (Table S1). The PCR product was integrated into pGEM-Teasy vector (Promega) and then cloned into vector pBI121 by XbaI and BamHI double digestion. Tobacco transformation through Agrobacteriumtumefaciens-mediated was performed as the leaf disk method described by Horsch et al. [35], and transgenic plants were screened by 50 mg/L kanamycin.

Southern Blot Analysis.
Genomic DNAs (about 25 g) were obtained from three independent T1 transgenic plant lines and double digested with restriction endonuclease EcoRI and BamHI (TaKaRa) at 37 ∘ C for 20 h and electrophoretically separated on a 0.8% agarose gel and transferred to positively charged nylon membrane. HindIIIdigested lambda DNA with digoxigenin labeling (Cat. 11218590910, Roche) was employed as marker. The DNA was fixed on the membrane by baking at 120 ∘ C for 30 min. According to the protocol of DIG-High Prime DNA Labeling and Detection Starter Kit (Roche, USA), all procedures including the preparation of probe, prehybridization, hybridization, and immunological detection were carried out in proper sequence.

Determination of APX, SOD, POD, and CAT Activity and
AsA Concentration. APX activity was determined according to the method of Nakano and Asada [36]. 0.4 g ground frozen fresh samples were homogenized in 4 mL of 50 mM extraction PBS buffer (pH 7.8) containing 0.2 mM EDTA and 2 mM AsA. The homogenate was transferred into a tube and centrifuged at 12,000 ×g for 20 min (4 ∘ C) and the supernatant was collected for APXase activity analysis. AsA content was determined according to the method of Arakawa et al. [37]. The crude extracts homogenized through grinding 0.3 g fresh samples macerated with liquid nitrogen in presence of 3 mL 5% (w/v) TCA into a fine powder were centrifuged at 4,000 ×g for 10 min at room temperature and the supernatant was collected for AsA content analysis. 0.2 g ground frozen fresh samples were homogenized in 10 mL precooling PBS buffer (pH 7.8), followed by being centrifuged at 12,000 ×g for 15 min (4 ∘ C), and the supernatant was extracted for enzyme activity analysis. SOD, POD, and CAT activities measurement was performed as described by Lee [38].

Biochemical and Physiological
Analyses. MDA content was determined by the thiobarbituric acid-(TBA-) based colorimetric method as described by Heath and Packer [39]. H 2 O 2 content was assayed by modified method according to Shi et al. [40]. 0.5 g fine grinding fresh samples were homogenized in an ice bath with 0.1% (w/v) TCA, and the homogenate was centrifuged at 12,000 ×g for 15 min. Then, 1 mL supernatant was mixed together with 1 mL potassium phosphate buffer (100 mM, pH 7.0) and 2 mL KI (1 M), and the absorbance was measured at 390 nm using a standard curve plotted with known concentration of H 2 O 2 . Relative electric conductivity was measured referring to the method of Zhang [41]. 0.5 g fresh leaf discs (0.5 × 0.5 cm) were cut into several conical flasks containing 25 mL distilled water and vacuumed for 8 min, 6 conical flasks totally, and the conical flasks were, respectively, treated under different temperatures lasting 20 min to determine the initial electric conductivity ( ) using Conductivity Meter (HI-9033). Then, the whole sample mixtures were heated with boiling water for 15 min to kill the cells and final electric conductivity ( 0 ) was determined after 2 h later, with electric conductivity of plant leaf dicks at 25 ∘ C as a control group (CK). Relative electric conductivity (REC) was evaluated as REC For photosynthetic analysis, a Chlorophyll Fluorescence Analyzer (Imaging-PAM-Maxi, Walz, Germany) was used to determine all of the parameters according to the manufacturer's instructions.

Molecular Cloning and Characterization of CaAPX.
A fragment (566-bp) was obtained by amplification of primers (Table S1) designed based on conserved regions, and sequence analysis showed that it was homologous to APX genes (not shown). The full-length sequence of CaAPX (GenBank Accession ID: KP635267) was retrieved by 3 and 5 -RACE amplification (primers listed in Table S1). CaAPX cDNA consisted of 1,017-bp nucleotides and encoded an open reading frame of 250 amino acid residues ( Figure S1).
The deduced amino acid sequence of CaAPX was aligned with homologous sequences from various species, including Arabidopsis thaliana, Gossypium hirsutum, Glycine max, Nicotiana tomentosiformis, Oryza sativa, Phoenix dactylifera, and Solanum tuberosum, using BLAST (https://blast.ncbi.nlm .nih.gov/Blast.cgi). The predicted structure of CaAPX dominantly contained alpha-helix and random coil, in which a POD domain containing the highly conserved amino acid sequences, namely, "XANX," "LPDAX," and "(E)RSGF/W" (Figure 1(b)), was found. These conserved motifs were shown to be critical to react with substrate AsA [42]. A neighborjoining bootstrap tree was constructed based on the homologous APX proteins sequences from various plant species (Figure 1(a)). We revealed that they were divided into four groups and CaAPX was categorized into a cytosolic-type APX protein (Figure 1(a)).

Expression Pattern of CaAPX in C. azalea.
We studied the expression patterns of CaAPX by qRT-PCR analysis in various tissues and different temperature stresses (Figure 1(c)). It was found that CaAPX was expressed in all tested tissues and the expression level of CaAPX in immature green fruit was the highest, with nearly 2.68 times higher than in mature leaf ( Figure 1(c)). Notably, the expression levels in immature green fruit and mature leaf were higher than other tissue types (leaf bud, flower bud, flower, seed, and petal) (Figure 1(c)). To investigate the expression of CaAPX under temperature stresses, we examined the transcription levels in leaves after exposing to 4 ∘ C and 38 ∘ C, respectively, with 5 time points.
We showed that the expression levels of CaAPX in leaves were both upregulated under the low and high temperature

Heterogeneous Expression of CaAPX Altered the Temperature Stress Tolerance and Displayed Elevated APX Activity and AsA Contents.
To investigate the molecular functions of CaAPX, we generated transgenic tobacco plants with heterogeneous expression. We identified 20 positive transgenic lines by kanamycin selection and gene-specific amplification (Table S1, Figure S2). Three lines were randomly selected for southern blotting analysis, which all confirmed the single insertion of target construct (Figure 2(a)). We quantified the expression levels of CaAPX in these three transgenic lines using qRT-PCR. The results showed that expression level in L2 line was the highest, approximately 3.83 times higher than in L1 line, and L3 line had a higher expression level of around 1.23-fold compared to L1 line (Figure 2(b)).
To study the tolerance to temperature stresses, transgenic plants were transferred into growth chamber at 4 ∘ C and 38 ∘ C, respectively. And phenotypes of treated plants were recorded every 15 min until significant wilting was emerged. After 8 h treatment under both 4 ∘ C and 38 ∘ C, the WT plants displayed obvious wilting symptoms, while transgenic lines displayed significantly enhanced performance (Figure 2(c)). The activity of APX at different temperatures was examined in transgenic and WT plants. It was found that APX activity was much higher in three transgenic lines than in untreated WT plants (at 0 h in Figures 3(a) and 3(b)). The APX activities showed a similar increasing pattern in all tested transgenic lines as well as WT plants, upon cold and hot treatments (Figures 3(a) and 3(b)); meanwhile the L2 line displayed higher activity than the rest of assayed plants at each time point (Figures 3(a) and 3(b)). We further measured the contents of AsA in WT and transgenic plants under treatments. The results showed that, before treatments (at 0 h), all transgenic lines had significantly higher levels of AsA than WT (Figures 3(c) and 3(d)). Upon both 38 ∘ C and 4 ∘ C treatments, the AsA levels were increasing along with treatment durations in all transgenic plants and WT (Figures  3(c) and 3(d)). Also the L2 line had the highest AsA levels than other assayed plants at each time point (Figures 3(c) and 3(d)). Their results indicated that ectopic expression of CaAPX caused increased APX activity and AsA levels and enhanced the plant tolerances to cold and hot stresses. Line L2 displayed highest APX activity and AsA content, which is consistent with the accumulation patterns of CaAPX transcripts in transgenic plants (Figure 2(c)).

Overexpression of CaAPX Triggered Upregulation of Antioxidant Enzyme Activities.
To study how the temperature stresses and CaAPX expression affected the ROS removing enzymes, the activities of SOD, POD, and CAT were measured. We showed that the SOD activities were upregulated before treatments (at 0 h in Figures 4(a) and 4(b)) in transgenic lines; there was a gradually increasing trend of SOD activity for each assayed plant during 38 ∘ C and 4 ∘ C treatments (Figures 4(a) and 4(b)); transgenic lines had higher SOD activities than WT at each timepoint of treatments (Figures 4(a) and 4(b)). The POD activities were upregulated in transgenic lines comparing to WT before treatments (at 0 h in Figures 4(c) and 4(d)), while the patterns of POD activities during treatments were different from SOD, which reached peaks at 8 h and declined at 12 h in transgenic plants (Figures 4(c) and 4(d)); in WT, the accumulation of POD activities was gradually increasing during 8 hours of treatments and decreased at 12 h (Figures 4(c) and 4(d)). The results of CAT analysis were similar to POD activities, except that CAT activities reached peaks at 8 h in all transgenic lines under both 38 ∘ C and 4 ∘ C treatments, and there was a sudden drop of activities at 12 h (Figures 4(e) and 4(f)). The activities of CAT in WT did not show strong induction under temperature treatments (Figures 4(e) and 4(f)).

Overexpression of CaAPX Reduced Accumulation of MDA, H 2 O 2 , and Electrolyte Leakage under Stresses.
To investigate the effects of altered ROS-scavenging activities (APX, CAT, and SOD) in transgenic plants, we measured the contents of MDA and H 2 O 2 and the rate of electrolyte leakage. We found that MDA levels in WT plants were immediately induced when exposed to the abnormal temperatures (4 ∘ C and 38 ∘ C) and accumulated more dramatically compared with transgenic lines. The MDA contents in transgenic lines were significantly decreased, accounting for 71.09% to 82.91% in comparison to WT plants (0 h at Figures 5(a) and 5(b)).
Although there was no significant difference in H 2 O 2 concentration between WT and transgenic plants incubated at room temperature (25 ∘ C), H 2 O 2 concentration in WT plants increased markedly under 4 ∘ C and 38 ∘ C temperature stresses (Figures 5(c) and 5(d)). In transgenic plants, the H 2 O 2 contents kept at the baseline level after 8 h treatments of temperature stresses (Figures 5(c) and 5(d)); but an increase at 12 h under 38 ∘ C was observed ( Figure 5(d)). In addition, the rate of electrolyte leakage was higher in WT plants than in transgenic lines under the temperature ranges from 15 ∘ C to -20 ∘ C ( Figure 5(e)). There was a slight rise in electrolyte leakage in all plants between 30 and 40 ∘ C ( Figure 5(f)), and the conductivity rate increased dramatically to the highest level at 60 ∘ C and 70 ∘ C ( Figure 5(f)), which suggested that the cells death of leaves had occurred since 60 ∘ C.

Parameters Associated with Chlorophyll Fluorescence in
Leaves under Temperature Stresses. The photosynthetic electron transport (PET) chain is a sensitive process associated with cellular ROS homeostasis. To understand the regulatory roles of CaAPX under stresses, we measured several parameters such as V/ and (II) associated with chlorophyll fluorescence. Before the stress treatments (0 h at Figures 6(a) and 6(b)), the V/ was nearly the same between WT and transgenic plants. Upon temperature stresses, V/ decreased markedly under both 4 ∘ C and 38 ∘ C temperature stresses; the decline in WT plants was much accentuated (Figures 6(a) and 6(b)). The (II) was slightly higher in the transgenic lines compared to WT plants under control conditions, and the trend for (II) was similar to V/ under temperature stresses (Figures 6(c) and 6(d)). After 12 h treatment, the figures for transgenic lines were approximately 1.32-and 1.12-fold for average in comparison to WT sampling, respectively, at 4 and 38 ∘ C. Therefore, it was suggested that low and high temperatures significantly descended the parameters associated with chlorophyll fluorescence in both genotypes, in which the effects of stresses were more noticeable on WT plants than on the transgenic lines.

Divergent Induction Patterns of ROS-Scavenging Genes upon Cold and Heat Stresses.
The elevated levels of ROS by temperature stresses are important signals to control the expression of ROS-scavenging enzymes [2,5,43]. To understand the ROS signal transduction in transgenic CaAPX plants under stresses, we analyzed the transcripts levels of tobacco ROS-scavenging genes, NtCu/Zn-SOD, NtCAT, NtD-HAR, and NtMDHAR, in response to temperature stresses. We showed that, before the temperature stress treatments, the expression of NtCAT was obviously upregulated in transgenic lines compared with WT plants (Figure 7(c)), while expression of NtCu/Zn-SOD, NtDHAR, and NtMDHAR was not significantly induced in CaAPX transgene plants. Upon cold and hot stresses treatments, the expression levels of all assayed genes were upregulated at some time points, but the patterns of induction between cold and hot stresses were mostly different (Figures 7(a)-7(h)). For instance, remarkable induction of NtCu/Zn-SOD was detected only at 2 h treatment of 38 ∘ C and expression dropped thereafter in CaAPX transgenic plants; under 4 ∘ C treatments moderate inductions were observed in both WT and transgenic plants (Figures 7(a) and 7(b)). In WT, the expression of NtCAT was induced significantly after 2 h of hot treatment (Figure 7(d)), while, in CaAPX transgenic plants, the expression of NtCAT did not change after 2 h and 4 h and greatly reduced at 8 h and 12 h of hot treatment. Under cold treatment, the expression of NtCAT was upregulated in both WT and transgenic plants (Figure 7(c)). These results suggested the CaAPX overexpression affected the ROS mediated regulation of SOD and CAT expression, and the hot and cold stresses had different responsive mechanisms of signaling. Meanwhile, the expression levels of NtDHAR and NtMDHAR displayed similar induction patterns between WT and transgenic plants under both cold and hot stresses (Figures 7(e)-7(h)), suggesting a different regulatory pathway was involved.

CaAPX Overexpression Plants Displayed Greater Thermotolerance in Seed Germination Assay.
To further investigate the thermotolerance effects of CaAPX in transgenic plants, we tested the germination of transgenic lines. The assayed lines were confirmed by T2 segregation analysis through PCR amplification of progenies ( Figure S3). No segregation was found for all three lines. Then we tested the germination rates of seeds on the MS medium containing antibiotics selection at 25 ∘ C, 4 ∘ C, and 38 ∘ C, respectively. We found that the germination rates of transgenic lines were a little higher         than from WT seeds at 25 ∘ C treatment but not significant (Figures 8(a), 8(b), and 8(c)).
Under 4 ∘ C and 38 ∘ C temperature stresses, the rates of transgenic lines were significantly higher than WT seeds (Figures 8(b), 8(d) and 8(c)). Particularly, under 4 ∘ C, almost no germination was found for WT plants, but the strong line (L2) showed a comparable rate to WT under normal condition (Figure 8(d)). The results indicated that overexpressing CaAPX significantly enhanced temperature stresses tolerance and improved the seed germination rate in transgenic lines under temperature stresses.

Discussions
Temperature stress, namely, low and high temperatures, often caused negative impacts on plant metabolism, cellular homeostasis, and major physiological processes [2]. Previous studies demonstrated that ROS is one of the main factors causing oxidative damage to plants exposed to environmental stresses [29]. Antioxidant enzymes play a significant role in eliminating toxic levels of ROS, generated during stress from living cells [44]. In stressful conditions, plants generally increased the APX activity and the elevated activity level usually correlates with increased stress tolerance [3]. During high and low temperature treatments, many physiological changes occur that result in increased expression of antioxidant enzyme genes to protect from oxidative stress. APX utilizes AsA and its specific electron to reduce H 2 O 2 to H 2 O, and overexpressing APX might play a critical role in growth and when subjected to high and low temperature. Previous research indicated that APX was highly responsive to temperature stresses and played an important role in the scavenging of ROS in plants. For example, the APX1 expression of A. thaliana significantly increased after being treated with heat stress [45]; Padaria et al. [46] found that TapAPX of a thermotolerant wheat cv. Raj3765 displayed up to 203-fold level of expression at 42 ∘ C heat stress exposure. The expression level of tAPX during cold acclimation of wheat was upregulated [47]; the expression of A. andraeanum APX mRNA was evidently elevated by cold stress [48]; OsAPX2 expression of Oryza sativa was also developmentaland spatial-regulated and was induced by cold stress [49]. In this study, the expression levels of CaAPX were both remarkably upregulated under low and high temperature treatment ( Figures 1(d) and 1(e)). It demonstrated that CaAPX was induced by heat and cold. We found that overexpression of CaAPX increased tolerance to temperature stresses, and transgenic tobacco grew better than wild type under 4 ∘ C and 38 ∘ C temperature stresses (Figure 2(a)). We showed that the activity of APX and the content of AsA were indeed remarkably enhanced in transgenic plants than those in wild type plants (Figures 3(a)-3(d)), suggesting their roles in scavenging H 2 O 2 (Figures 5(c) and 5(d)). Previous studies indicated that antioxidant enzyme activities were tightly associated with the expression of antioxidant enzyme genes, which could be upregulated after the plants were treated with temperature stresses. For instance, Lin et al. [50] reported that CAT and APX expression profiles were well matched with the data for CAT and APX enzyme activities in the broccoli and Chinese cabbage plants, respectively. And overexpressing chloroplast APX in cotton has conferred higher photochemical activity of photosystem II (PSII) and stronger antioxidant capacity to transgenic plants [51]. Overexpression of CaAPX also enhanced the activities of SOD, POD, and CAT in transgenic tobacco plants than that in the wild type plants under 4 ∘ C and 38 ∘ C temperature stresses (Figures 4(a)-4(f)). Furthermore, it was demonstrated that the high activities of SOD, POD, and CAT could result in producing the high level of AsA, which could alleviate photoinhibition to protect the plants [52]. Moreover, the high level of AsA could detoxify ROS effectively, further reduce the damage to membrane lipids, enhance the repair of PSII, and protect photosystem. Zhou et al. [53] concluded that V/ is an early indicator of temperature stress tolerance in tomato and that this provides effective and reliable information about leaf photosynthetic performance. Hence, several parameters such as V/ (Figures 6(a) and 6(b)) and (II) (Figures 6(c) and 6(d)) associated with chlorophyll fluorescence in this study were decreased under extreme temperature stresses, but these effects were more accentuated in wild type plants. These results manifested that CaAPX is a key component to balance the cellular ROS homeostasis and could be an ideal target for improving tolerance to temperature stresses.
The transcript levels of NtCu/Zn-SOD, NtCAT, NtDHAR, and NtMDHAR genes significantly increased on exposure to stress in transgenic tobacco plants (Figure 7). The critical role of APX in stress tolerance and upregulation of Cu/Zn-SOD, CAT, DHAR, and MDHAR transcripts have been reported [54] under stress. Variations in physiological parameters potentially contribute to the complex phenotype of cold and heat tolerance; however, a clear relation between stress and coexpression of antioxidant genes has so far not been evident. In this study, the expression pattern of NtCu/Zn-SOD and NtCAT in times was specific to two genotypes and showed no direct correlation with the corresponding enzymes activity. This result was likely because of the complex regulation mechanisms of gene expression; gene expression cannot be directly correlated with enzyme activity [55].
CaAPX was expressed in all tested tissues (leaf, leaf bud, flower, flower bud, petal, immature green fruit, and seed embryo) but was much more abundant in the immature green fruit relative to other tissues (Figure 1(c)). Wang et al. [56] showed that a wide range of MaAPX1 transcript expression levels among the different tissues in banana, with relatively strong expression, was observed in leaves and roots. Our results indicated that CaAPX might have different functions in different tissues during the growth and development of C. azalea. To test the thermotolerance and cold-tolerance of transgenic plants, we employed a seed germination assay. Sun et al. [57] reported that overexpression of StAPX improved seed germination, and Faize et al. [58] showed that overexpression of cytapx also promoted seed germination and germination rates. In our conditions, we found that overexpressing CaAPX significantly enhanced temperature stresses tolerance (Figures 8(a), 8(b), and 8(c)) and improved the seed germination rate in transgenic plants (Figure 8(d)) under hot and cold stresses, which was consistent with the other previous plants. Overall, this work characterized the functions of CaAPX in response to heat and cold stresses. The results provided comprehensive information about the orchestration of cell ROS homeostasis mediated by CaAPX, which could give some insights into rational engineering of plants toward temperature tolerances.