Effects of Juniperus phoenicea Hydroalcoholic Extract on Inflammatory Mediators and Oxidative Stress Markers in Carrageenan-Induced Paw Oedema in Mice

Juniperus phoenicea (J. phoenicea) is a wild tree belonging to the Cupressaceae family, commonly used for the treatment of several disorders. This study aimed to evaluate the potential protective effects of J. phoenicea hydroethanolic extract (EtOH-H2OE) against oxidation, acute inflammation, and pain in mice models. For the purpose, chemical compounds of J. phoenicea EtOH-H2OE were also analyzed by GC-MS. The J. phoenicea EtOH-H2OE showed a potent antioxidant activity in vitro, thanks to its richness in phenolic and flavonoid compounds. Mice treated with EtOH-H2OE (100 mg/kg BW) showed reduced paw oedema formation and decreased malondialdehyde (MDA) content. The evaluation of antioxidant enzyme activities in paw oedema tissue after five hours of carrageenan induction showed a significant increase (P < 0.05). Inflammatory biomarkers explorations of J. phoenicea EtOH-H2OE-treated mice showed a restoration of the studied parameters to near-normal values. Furthermore, EtOH-H2OE of J. phoenicea produced a significant reduction of the number of abdominal writhes (P < 0.05) in a dose-dependent way. Phytochemical analysis of the J. phoenicea EtOH-H2OE by GC-MS showed the presence of hexadecanoic and stearic acids known as anti-inflammatory and analgesic compounds. Our investigation provided evidence that J. phoenicea EtOH-H2OE can effectively reduce the inflammation and pain in mice models.


Introduction
Medicinal plants have been used in traditional health care systems since prehistoric civilizations. World Health Organization (WHO) estimates that about 85% of the worldwide traditional knowledge involves the use of plant extracts [1]. Therefore, treatment of various diseases has been carried out with medicinal plants for many years. These plants are an endless source of pharmaceutical and therapeutic products, thanks to their richness in bioactive molecules that have medicinal properties. The antioxidant compounds derived from plant extracts could be a solution to overcome diseases, caused by oxidative stress, such as inflammation [2].
Oxidative stress is the result of an imbalance between the reactive oxygen species (ROS) levels production during metabolism and their removal by the antioxidant defense systems [3]. Excessive production of ROS in tissues may contribute to cell damage by altering cellular molecules, namely, proteins, lipids, and DNA/RNA [4,5]. Furthermore, it was indicated that ROS excess initiates the inflammation process by stimulating the induction of proinflammatory cytokines, chemokines, and pro-inflammatory transcription factors causing tissue injury [6]. Inflammation represents a rapid yet coordinated set of events that enable tissues to respond to injuries or infections [7]. It is a natural defense mechanism that involves the recruitment of immune cells and the overproduction of inflammatory cytokines into the tissues in response to the ROS release [8]. To avoid the ROS harmful effects, human cells have developed a sophisticated antioxidant defense system that consists of an enzymes system involved in the conversion of ROS to less reactive molecules such as O 2 and water [9]. Superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) are the main endogenous enzymes involved in protecting aerobic cells from the noxious effects of ROS. They give protection by directly scavenging superoxide radicals and hydrogen peroxide, converting them into stables species [10]. Cell damage induced by an excessive production of ROS depends on the potential of the cellular antioxidant enzymes to neutralize them [11]. Studies documented that, in addition to the enzymatic/nonenzymatic cellular antioxidant defense system's protective effects, natural products having antioxidant activities may lead to delaying the oxidative stress and inflammatory tissue damage by enhancing the cell/tissues defenses [12].
The search for natural products with antioxidant activities has increased enormously over the last decades. Plants would be a promising alternative to reduce oxidative damage, thanks to their secondary metabolites richness such as polyphenols, flavonoids, tannins, terpenoids, and anthraquinones [13]. It was reported that plant natural compounds are able to interact with ROS and thus terminate the chain reaction before the cell vital molecules were seriously damaged [14].
Juniperus phoenicea is a wild tree belonging to the Cupressaceae family and is popularly known as "Arâar" in Tunisia. Juniperus species have been widely used in the traditional medicines against various infectious and inflammatory diseases such as cold, diarrhea, fungal infections, hemorrhoids, leucorrhea, diabetes, and wounds [15]. In Tunisian folk medicine, the J. phoenicea leaf decoction was frequently employed to regulate menstruation and relieve the pain of menstrual cramps [16]. The mixture of berries and leaves of this plant was used traditionally as antidiabetic remedies [17]. Previous studies on the biological activities of J. phoenicea have mainly focused on the essential oils. Although the plant is claimed to be used for its anti-inflammatory and analgesic effects by Tunisian traditional healers, no published work so far investigates the use of J. phoenicea for this purpose. J. phoenicea has a long use record in the treatment of oedema in the Mediterranean subdivision [15].
This study aimed mainly to (1) explore the properties of J. phoenicea EtOH-H 2 OE in the management of inflammation and oxidation using carrageenan-induced paw oedema model, (2) evaluate the analgesic potential of J. phoenicea EtOH-H 2 OE using acetic acid-induced abdominal contraction, (3) identify the phytochemicals present in the extract by GC-MS, and (4) assess the relationship between the extract agents and the bioactivities.  [18] and Harborne [19]. They were based on the visual observation of color change of EtOH-H 2 OE of J. phoenicea. The tested phytochemicals are phenolics, flavonoids, anthraquinones, glycosides, terpenoids, tannins, saponins, and alkaloids. The results are expressed as "+" for the presence and "-" for the absence of phytochemicals.

Gas Chromatography-Mass Spectrometry (GC-MS) Analysis.
GC-MS analysis of EtOH-H 2 OE of J. phoenicea was performed with an Agilent 6890N Network GC System (Agilent Technologies). The system was equipped with an HP-5 MS column having 30 m × 0.25 mm i.d. × 0.25 m film as dimensions. The used system was coupled to a mass selective detector and the carrier gas was helium. The GC oven temperature started at 40 ∘ C and was held at 60 ∘ C for 2 min and was then programmed to rise from 60 to 325 ∘ C at a rate of 5 ∘ C/min. The injected extract temperature was set at 280 ∘ C.
The components identification was achieved by careful examination of fragmentation patterns and spectral data obtained from the Wiley and NIST libraries. This determination was carried out in duplicates.

Phenolic Contents and Antioxidant Activity
2.6.1. Determination of Total Phenols. The total phenolic content was assayed using the Folin-Ciocalteu reagent and gallic acid as a standard [20]. The absorbance was measured at 760 nm and the results were expressed as mg of gallic acid equivalent per g (mg GAE/g). The assays were performed in triplicates.

Determination of Total
Flavonoids. The total flavonoid content in EtOH-H 2 OE was estimated by the aluminum chloride spectrophotometric method [21]. The mixture absorbance was read at 430 nm and the result was expressed as mg of quercetin equivalent per g dry weight (mg QE/g). Tests were performed in triplicates.

Determination of DPPH Radical Scavenging and -
Carotene Bleaching Capacity. The free radical scavenging capacity and the ability of the EtOH-H 2 OE to avoidcarotene bleaching were determined using the methods described by Kirby and Schmidt [22] and Pratt [23], respectively. The ascorbic acid and the butylated hydroxytoluene (BHT) were taken as references.  (2000) with some modifications. Healthy mice (40 g) were randomly divided into four groups (n = 6). The control group (group I) received only a water solution, while groups II, III, and IV were treated with EtOH-H 2 OE of J. phoenicea at doses of 100, 200, and 400 mg/kg body weight, respectively. Following the fasting period, the graded doses of EtOH-H 2 OE of J. Phoenicea were administered orally by gavage. The animals were maintained on standard animal diet and water. All animals were daily observed for behavioral pattern, body weight, and physical appearance changes and checked for mortality during the 2-week observation period.

Anti-Inflammatory Test: Carrageenan-Induced Paw
Oedema. Anti-inflammatory activity of EtOH-H 2 OE of J. phoenicea was assessed according to the method described by Ravi et al. [24] with some modifications. Mice with a mean weight of 35 g were split into four groups (n = 6): (i) Group I: mice were pretreated with 1 ml/kg of sterile saline solution by subplantar injection and had no inflammation (control group) (ii) Group II: mice were inflamed by carrageenan injection 1% and did not receive any treatment (Carr) (iii) Group III: mice received dexamethasone (100 mg/Kg BW) by intraperitoneal injection and were considered as standard (Carr + DEX) (iiii) Group IV: mice were given 100 mg/kg BW of EtOH- Paw oedema was induced [25] by administration of 50 L of 1% w/v carrageenan solution (type IV, Sigma Chemical Company, USA) into subplantar tissues of the right hind paw of each animal after one hour of intraperitoneal administration of plant extract and drug.
The swelling of carrageenan injected paw was measured before and 1, 2, 3, 4, and 5 hours after the induction using a Digital Vernier Caliper [26]. The anti-inflammatory activity was calculated as percentage inhibition of oedema in the extract-treated animals under test in comparison to the carrageenan control group.
where PT represents the oedema volume of the drug-treated group and P0 is paw oedema of the carrageenan-treated group.

Blood Sampling.
Five hours after the carrageenan administration, the animals were anesthetized and the blood samples were collected in heparin tubes. Plasma samples were obtained after centrifugation (15 min at 4000 rpm) and they were kept in −20 ∘ C until further analysis. The pellet obtained after centrifugation on heparin will be used for the assays of the oxidative status markers.

Exploration of Inflammatory Biomarkers
(1) Hemogram Test. The measurement of white blood cells and platelets is carried out with a hematology analyzer (KX21 hemogram).
(2) Determination of C-Reactive Protein. The serum C reactive protein levels were analyzed by an automatic analyzer "COBAS INTGRA 400." The CRP is expressed in mg/L. (4) Exploration of Oxidative Stress Parameters In Vivo. The oxidative stress parameters were determined in skin tissues paw oedema. Homogenates were diluted (10%, w/v) in a Trisbuffered saline (pH 7.4) and centrifuged for 25 min (9000 rpm). The obtained supernatants were used for the determination of MDA as reported by Draper and Hadley [28]. The superoxide dismutase (SOD) content was colorimetrically assessed as reported by Beyer and Fridovich [29] using the inhibition of nitro blue tetrazolium (NBT). The catalase (CAT) activity was evaluated according to the method of Aebi [30] using H 2 O 2 as substrate. The glutathione peroxidase (GPx) content was analyzed according to the method of Flohe and Günzler [31] and expressed as mol GSH/min/mg protein.
(5) Histopathological Assessment of Skin Tissue. Tissue specimen samples from subplantar muscles of all the studied groups were dissected five hours after carrageenan injection for histological examination. They were fixed in formalin solution (10%) and stained with hematoxylin-eosin. The sections were finally observed under a light microscope and photographed with an Olympus U-TU1X-2 camera.
2.9. Acetic Acid Writhing Test. The acetic acid-induced writhing test was performed as previously reported by Reza et al. [32] with some modifications. The experimental study designed six groups of 6 mice. Group I served as control and received distilled water. Groups II and III were treated with diclofenac sodium (standard analgesic drug) at 50 and 100 mg/kg, respectively. Groups IV, V, and VI were administered 50, 100, and 150 mg/kg of the EtOH-H 2 OE of J. phoenicea, respectively. After 30 minutes, acetic acid (1 mL of 1% w/v) was administered with intraperitoneal injection for writhing induction. The total number of abdominal constrictions was counted for each group of mice after 15 minutes of acetic acid injection for the period of 5 minutes.
The protection percentage against acetic acid was calculated using the following formula: 2.10. Statistical Analysis. Data were expressed as mean values ± standard deviation (SD). A statistical significance comparison between groups was accomplished using the SPSS version 20. The mean differences between the different groups were assessed by Duncan and Tukey's post hoc tests and compared using one-way analysis of variance (ANOVA). Differences were considered significant at P < 0.05.

Phytochemical Analysis of Organic Extract.
The phytochemical screening of hydroethanolic extract of J. phoenicea leaves revealed the presence of various secondary metabolites such as terpenoids, tannins, saponins, alkaloids, and anthraquinones, while glycosides were not detected in the tested extract (Table 1). Furthermore, the quantitative estimation of the total phenolic contents showed that the EtOH-H 2 OE contains the highest amount of phenolics (70.30 mg GAE/g) and total flavonoid contents (11.33 mg QE/g) ( Table 2).

J. phoenicea EtOH-H 2 OE GC-MS Analysis.
The identities of J. phoenicea constituents, their contents (%), and their retention times are listed in Table 3. The analysis of EtOH-H 2 OE of J. phoenicea leaves revealed the presence of 25 compounds belonging to different chemical classes. The compounds, hexadecanoic acid (7.41%), stearic acid (5.69%), eseroline (3.15%), and azelaic acid (2.45%), were the most abundant. This extract also contains some sugars including -D-Glucopyranose (1.22%), L-Fructose (0.23%), and .-D-Galactopyranoside (0.17%). Besides, EtOH-H 2 OE harbors some phenolic components including gallic, caffeic, andcoumaric acids. EtOH-H 2 OE of J. phoenicea leaves revealed the presence of a considerable amount of saturated fatty acids (18.42 % of the total FAs). Table 2, the DPPH radical scavenging activity was appreciated by the determination of the IC 50 values. EtOH-H 2 OE of J. phoenicea exhibited a remarkable free radical scavenging activity with an IC 50 value of 12.22 g/ml when compared to vitamin C (IC 50 = 3.5 g/l). In the -carotene bleaching assay, the extract showed a potent activity (IC 50 = 15 g/l) less than that of the BHT (5.1 g/l) taken as reference (Table 2).

Antioxidant Activity. As shown in
A positive correlation between total phenolics content and antioxidant property (R 2 = 0.972) was noted.

Acute Toxicity Study.
EtOH-H 2 OE of J. phoenicea up to the dose of 400 mg/kg BW did not produce any signs of adverse reactions and no changes in the behavior of the treated animals up to 14 days following the extract administration. The treated animals did not display any abnormal signs such as food and water intake, convulsions, salivation, or diarrhea. No deaths or weight losses were recorded during the study. Therefore, EtOH-H 2 OE of J. phoenicea at 100 mg/Kg BW was used in the in vivo investigation of the anti-inflammatory activity.

Effects of J. phoenicea Leaf Extract on
Carrageenan-Induced Paw Oedema

Inhibitory Effect of the Extract on Paw Oedema In
Vivo. The comparative inhibitory effects following treatment with EtOH-H 2 OE of J. phoenicea and dexamethasone on carrageenan-induced paw oedema are presented in Table 4 and Figure 1. The subcutaneous administration of carrageenan generated an increase in the paw size in mice due to oedema. This paw oedema peaked after 3 hours of experiment, especially for the untreated group, thus indicating an acute paw inflammation. The experimental data of the treated group by EtOH-H 2 OE of J. phoenicea showed a significant decrease in the paw oedema size (P < 0.05), which was time-dependent and more important than the standard drug dexamethasone. Our findings revealed an inhibition of 77.5% of the paw oedema using the EtOH-H 2 OE compared to an inhibition of 57.89% using the standard drug after five hours. EtOH-H 2 OE of J. phoenicea (100 mg/kg BW) displayed a significantly (P < 0.05) higher anti-inflammatory capacity when compared to dexamethasone after 5 hours.

Hematological, Biochemical, and Histopathological
Examination. The macroscopic results were confirmed through hematological, biochemical, and histopathological explorations. The hematological parameters were assessed by monitoring the white blood cells and blood platelet levels. A significant increase in white blood cell count and blood platelets was shown in carrageenan inflamed group compared to the negative control group (Table 5). However, the inflamed animals, treated with the EtOH-H 2 OE of J. phoenicea, showed a significant decrease in the white blood cells and blood platelet counts and were close to those of control and dexamethasone-treated mice.    Values represent mean ± SD ( = 6) in each group. * represents P < 0.05, * * represents P < 0.01, and * * * represents P < 0.001; # represents P < 0.05, ## represents P < 0.01, and ### represents P < 0.001; * compared to control group and # compared to Carr + DEX. Control: physiological water, Carr: carrageenan, Carr + J: carrageenan + J. phoenicea, EtOH-H 2 OE, Carr + DEX: carrageenan + dexamethasone.  As shown in Table 4, the administration of carrageenan led to a significant enhancement in CRP level for the carrageenan group compared to the control animals. However, the CRP level significantly decreased (P < 0.05) in the groups treated with J. phoenicea (62.5%) and dexamethasone (50%) when compared to the carrageenan untreated group.
The fibrinogen rate decreased significantly (P < 0.05) in the groups of mice treated with the EtOH-H 2 OE of J. phoenicea (38.3%) and dexamethasone (37.42%) compared to the carrageenan untreated group (Table 5).
Biopsies from plantar muscles of the untreated group showed normal histological sections ( Figure 2). However, biopsies from carrageenan-treated mice (Figure 2(a)) showed numerous inflammatory cells and tissue structure disruption. Biopsies from the J. phoenicea-treated mice (Figure 2(d)) showed absence of inflammatory cells.

In Vivo Effects on the Malondialdehyde (MDA) Levels.
The malondialdehyde levels in oedema paw induced by carrageenan are shown in Table 6. For the carrageenan-treated mice, the dermal MDA levels increased significantly (P < 0.001) compared to the normal control group. Nevertheless, the treatment with 100 mg/kg BW of EtOH-H 2 OE showed a significant decrease (P < 0.001) compared to the carrageenan group and even the dexamethasone group (10 mg/kg). The treatment with J. phoenicea reinstated the MDA formation by 95.37% against only 90.74% recorded in dexamethasone. The malondialdehyde concentrations in the J. phoenicea EtOH-H 2 O-treated group were comparable to those of the normal control group.

Effects on Enzymatic Antioxidant Status.
The obtained results of SOD, CAT, and GSH levels in the paw oedema tissue of the different tested groups are summarized in Table 6. The carrageenan-induced inflammation mice led to a significant decrease of the dermal SOD, CAT, and GSH levels compared to the normal control mice (P < 0.001). However, our results proved that the animals pretreated with J. phoenicea EtOH-H 2 OE did not produce any significant differences in enzymatic antioxidant levels compared to the control group.  Values are mean ± SE for six rats in each group. * * * represents P < 0.001; * * represents P < 0.01; * represents P < 0.05. SOD: superoxide dismutase, CAT: catalase, GSH: glutathione peroxidase, MDA: malondialdehyde. * * * (P < 0.001) represents highly significant difference in comparison with control mice. * * (P < 0.01) represents moderately significant difference in comparison with control mice. * (P <0.05) represents significant difference in comparison with control mice. ### (P <0.001) represents highly significant difference in comparison with Carr group. ## (P < 0.01) represents moderately significant difference in comparison with Carr group.  Values are expressed as mean ± SD (n = 6). a,b,c,d,e,f Different letters in the same column indicate significant differences The treatment of inflamed mice with the EtOH-H 2 OE of J. phoenicea (100 mg/kg BW) restored the SOD activity by 84.24%, the CAT activity by 91.17%, and the GPx activity by 79.28%, compared to the control group. The results showed that the J. phoenicea leaves hydroethanolic extract displayed a protection of 84.24%, 91.17%, and 79.28% for SOD, CAT, and GSH activities, respectively, compared to 78.79%, 61.34%, and 77.85% using dexamethasone as reference drug towards inflammation induced by carrageenan.

Acetic Acid-Induced Writhing Response.
Our results showed that the tested extract at different doses and diclofenac sodium used as a standard significantly reduced the writhing number as compared to the control untreated mice (P < 0.05). As shown in Table 7

Discussion
Plants have been commonly used for centuries as potential sources of new anti-inflammatory compounds. Inflammation is a physiological protective response to body tissue injury or bacterial invasion, which involves several intricate factors. During the inflammatory response, the injured tissues release mediators leading to the generation of ROS, which are well known for their deleterious effects [33]. The antiinflammatory effects of numerous plant extracts may be attributed to the antioxidant property of their phytoconstituents. In the current study, the J. phoenicea EtOH-H 2 OE was found to have potent antioxidant activity in vitro. The richness of EtOH-H 2 OE of J. phoenicea in phenolics known for their powerful antioxidant property and also involved in the modulation of pain and reduction of inflammation [34][35][36] motivated us to evaluate the analgesic and antiinflammatory effects of J. phoenicea in mice models.
The paw oedema induced by carrageenan is a model often used to assess the anti-inflammatory effects of novel compounds. This inflammation type has two major phases. The first is caused by histamine, leukotriene, kinin, and cyclooxygenase release during the first hour after the carrageenan administration. The second or delayed phase is linked to the generation of prostaglandins, bradykinin, and neutrophil infiltration [37]. In the present investigation, the significant inhibitory activity (P < 0.001) shown by EtOH-H 2 OE of J. phoenicea over a period of 5 h in carrageenan-induced inflammation was higher than that elicited by dexamethasone. Dexamethasone is a prostaglandin synthesis inhibitor [38]. Our results suggest that the anti-inflammatory effect of EtOH-H 2 OE of J. phoenicea may be due to the inhibition of prostaglandin biosynthesis, which is similar to that produced by steroidal anti-inflammatory drugs such as dexamethasone. However, exact mechanism of inhibition of prostaglandin synthesis could be a potential future perspective.
Carrageenan-induced local inflammation was reported to be associated with the generation of reactive oxygen species (ROS) and to play a key role in the genesis of oxidative stress [39]. Previous research has reported that ROS overproduction may lead to increasing lipid peroxidation [40]. A reduction of the MDA level, a biomarker involved in lipid peroxidation, was observed in EtOH-H 2 OE of J. phoenicea treated group compared to the untreated group after 5 hours. According to the enzymatic antioxidant analysis, J. phoenicea may promote the activities of SOD, CAT, and GSH by boosting a cellular antioxidant protection mechanism. This implies a protective effect of J. phoenicea extract by stimulating the expression and the activity of antioxidant enzymes during the inflammatory process.
These results suggest that the extract contains some phytochemical compounds with antioxidant activities which could contribute to the anti-inflammatory process [41,42]. Boughton-Smith et al. [43] showed that the carrageenan-induced rat paw oedema tissue is sensitive to antioxidants. Therefore, antioxidant components in EtOH-H 2 OE of J. phoenicea may also contribute to its anti-inflammatory capacity.
It is well known that following the carrageenan-induced rat paw oedema there are changes in blood parameters that are suggestive of an acute phase reaction leading to a hemostatic imbalance [44]. Carrageenan-induced increase in total white blood cells count and platelets occurs at the site of inflammation due to the release of inflammatory cytokines, which increases the recruitment of neutrophil counts [45]. The reduced count of total white blood cells could be the result of an inhibition of carrageenan-induced total leukocytes, which occurs at the site of inflammation by the phytoconstituents of the hydroethanolic extract of J. phoenicea.
It is well established that dexamethasone and other antiinflammatory drugs inhibit migration of inflammatory cells by inhibiting the release of various chemical mediators [46]. In the present study, the significant decrease in the WBC count caused by the EtOH-H 2 OE of J. phoenicea and dexamethasone suggests that these agents are able to reduce inflammation by decreasing the leukocyte migration to the tissue injury sites and possible inhibition of cellular infiltration such as neutrophils and granulocytes.
Platelets have been recognized not only to play a major role in hemostasis but also to participate in inflammation and innate and adaptive immunity responses [47]. It was reported that in the inflamed paw tissue there is platelet aggregation and fibrin deposition [48]. It has been proven that the platelets form aggregates with leukocytes and interact with neutrophils, monocytes, and lymphocytes and therefore play a critical role in the inflammatory process [49]. The decrease in platelet count, observed in EtOH-H 2 OE treated group, suggests a possible inhibitory effect of J. phoenicea components on platelet aggregation, which could contribute to the anti-inflammatory process [50].
Most inflammatory biomarkers such as fibrinogen and CRP increased significantly following the inflammation response. However, EtOH-H 2 OE of J. phoenicea significantly decreases the mean of fibrinogen and CRP levels. All these findings were also confirmed by histological examination. Indeed, the extract considerably decreases the number of cellular infiltrates and reduces oedema tissue as did reference drug dexamethasone, whereas the paw tissue of untreated mice displayed a subcutaneous oedema with invasive cell infiltration associated with an epidermal ulcer and vascular congestion compared to the treated ones.
The acetic acid-induced writhing protocol was achieved to assess the peripheral analgesic efficiency of test drugs. According to the test, the extract has significantly (P < 0.001) decreased the total number of abdominal constrictions by boosting pain inhibitory mediators. The pain sensation is elicited by producing localized inflammatory response due to the release of endogenous substances as well as some other pain mediators such as arachidonic acid via prostaglandin biosynthesis [51]. Prostaglandin products, at damaged tissue sites, contribute to the inflammatory process and pain by increasing the capillary permeability [52]. Our results suggest that J. phoenicea contains some phytochemical compounds that can exert anti-inflammatory and antinociceptive effects probably by blocking the release of inflammatory mediators like serotonin, histamine, and prostaglandin and reducing blood flow.
Thus, the anti-inflammatory and analgesic activities are due to the individual or synergistic effect of the J. phoenicea components. The richness of J. phoenicea extract in terpenoids, polyphenols, and flavonoids may also synergistically promote inhibition of the enzymes involved in the prostaglandin synthesis as previously reported [53,54].
The GC-MS analysis of J. phoenicea EtOH-H 2 OE revealed the presence of hexadecanoic acid in this extract. Earlier reports have evaluated the efficiency of hexadecanoic acid in the anti-inflammatory process and reported that hexadecanoic acid, in studies with isolated Kupffer cells, improved the inhibition of various chemical mediators such as nitric oxide, interleukin-10, tumor necrosis factor-, and prostaglandin E2 [55]. Moreover, recent observations highlighted the anti-inflammatory effects of azelaic acid achieved by reducing reactive oxygen species [56]. On the other hand, the tested extract contained a significant amount of stearic acid. It was reported that these fatty acids fairly produce potent anti-inflammatory and analgesic effects [57]. Our results also suggest that J. phoenicea contains promisingly potent phytochemical compounds with anti-inflammatory and antinociceptive properties, which may inhibit the release of inflammatory mediators. EtOH-H 2 OE of J. phoenicea may have a potential benefit for the management of pain and inflammatory disorders. These results can justify the traditional use of this plant as a decoction to relieve the pain in Tunisia [16].

Conclusion
The current study revealed that the hydroethanolic extract obtained from J. phoenicea has anti-inflammatory and analgesic activities that were coupled with potent antioxidant capacity attributed to the total extract richness in various chemical compounds. Further studies are required to confirm our results for the use of this extract as a source for new proinflammatory drugs in human beings.

Data Availability
No data were used to support this study.

Conflicts of Interest
The authors declare that there are no conflicts of interest.