Contrast Media & Molecular Imaging
Volume 2019 (2019), Article ID 1845637, 13 pages
https://doi.org/10.1155/2019/1845637

Review Article

Nanoparticle-Based Paramagnetic Contrast Agents for Magnetic Resonance Imaging

Juan Pellico, Connor M. Ellis, and Jason J. Davis

Department of Chemistry, University of Oxford, South Parks Road, Oxford OX1 3QZ, UK

Correspondence should be addressed to Jason J. Davis; jason.davis@chem.ox.ac.uk

Received 12 February 2019; Accepted 4 April 2019; Published 5 May 2019

Guest Editor: Paulo H. Rosado-de-Castro

Copyright © 2019 Juan Pellico et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract.

Magnetic resonance imaging (MRI) is a noninvasive medical imaging modality that is routinely used in clinics, providing anatomical information with micron resolution, soft tissue contrast, and deep penetration. Exogenous contrast agents increase image contrast by shortening longitudinal (T₁) and transversal (T₂) relaxation times. Most of the T₁ agents used in clinical MRI are based on paramagnetic lanthanide complexes (largely Gd-based). In moving to translatable formats of reduced toxicity, greater chemical stability, longer circulation times, higher contrast, more controlled functionalisation and additional imaging modalities, considerable effort has been applied to the development of nanoparticles bearing paramagnetic ions. This review summarises the most relevant examples in the synthesis and biomedical applications of paramagnetic nanoparticles as contrast agents for MRI and multimodal imaging. It includes the most recent developments in the field of production of agents with high relaxivities, which are key for effective contrast enhancement, exemplified through clinically relevant examples.

1. Introduction

Noninvasive imaging techniques support disease diagnosis and pathological characterisation with ease in a comparatively safe manner [1, 2]. These techniques include nuclear imaging modalities such as positron emission tomography (PET), single photon emission computed tomography (SPECT), computed tomography (CT), optical imaging (OI), ultrasound (US), and magnetic resonance imaging (MRI). All these present individual distinct advantages, as well as limitations which need to be considered within the aims of the specific study. MRI is routinely utilised because of its associated intrinsic high spatial resolution, deep tissue penetration, and three-dimensional anatomical information [3]. It is, thus, widely employed in clinics for the diagnosis and prognosis of a broad range of disease states. Despite this, MRI suffers from inherently low sensitivity; hence, exogenous contrast agents are applied to overcome this obstacle by shortening the relaxation times of bulk water [4]. Contrast agents not only enhance image contrast but also support multimodal imaging. They can be classified into two different groups depending on the operational mode. T₁, also called positive contrast agents, shortens longitudinal relaxation times and brightens the accumulation area [5]. T₂, or negative contrast agents, conversely shortens transversal relaxation times and darkens the immediate and surrounding area. From metal complexes to nanoparticles, different formulations have been employed as contrast agents for MRI with most of these based on the use of highly paramagnetic ions such as Gd³⁺, Mn²⁺, and Fe³⁺ [6]. They are usually utilised as coordination complexes with acyclic or cyclic chelate agents to reduce associated toxicity of the free metal ion. Although these molecular agents have been extensively used, paramagnetic metallic complexes have problems related to their fast excretion [7]. In terms of targeting and adding additional imaging modes, the use of low molecular weight lanthanide complexes is limited by high synthetic demand and the possibility of undesirable side reactions occurring during the synthesis. For these reasons, paramagnetic contrast agent research has focused on the development of nanoparticulate forms over the last few years [8, 9]. Paramagnetic nanoparticles present several advantages over traditional coordination complexes. Composition is readily tuneable as is size and shape. Magnetic characteristics are improved by geometric local density effects rendering markedly higher T₁ and/or T₂ relaxometric values than the corresponding coordination complexes. In addition, control over associated pharmacokinetics enables an increase in blood circulation time [10]. Ultimately, most nanoparticulate platforms are cleared by the reticuloendothelial system (RES) with the action of macrophages, such as Kupffer cells, generating uptake from the bloodstream to the liver and spleen. The rate of this process, known as opsonization, depends on the size of the nanoparticle and its chemical coating. Nanoparticles below 5–7 nm in diameter are able to pass through the kidney glomerulus triggering fast excretion through urine. Larger particles are excreted by the RES route with kinetics that can be tuned through the surface chemistry. The protein corona that develops on these particles during circulation is key to this and can be controlled by pegylation, for example, or a polymer coating such that blood circulation time is optimised [11]. This has a direct role in MRI acquisition, where a greater acquisition time increases signal-to-noise and consequently “image quality.” Another important feature within the use of nanoparticles is their high surface-to-volume ratio, supporting a high ligand (protein, antibody, and peptide) payload as well as tuneable and potentially accessible internal volume [12]. These features enable combination with drugs such that targeted, imaging-based, diagnosis may be followed by simultaneous therapy specific to that condition, known as theranostics [13].

Many different approaches have been used to develop paramagnetic nanoparticles for MRI, a few of which will be represented herein. In general, one of two synthetic strategies is followed: (1) formation of nanoparticles with the paramagnetic ion incorporated into the nanostructured framework and (2) postfunctionalisation of the particles with a lanthanide coordination complex. The first approach has been largely based on the synthesis of stoichiometric or nonstoichiometric nanoparticulate metal oxides such as Gd₂O₃, MnO, Mn₃O₄, Dy₂O₃, or γ-Fe₂O₃. This approach also includes NaGdF₄, KGdF₄, β-NaDyF₄, NiFe₂O₄, and ZnFe₂O₄ nanoparticles as well as others [14–19]. The second strategy has been developed with a number of supporting nanoparticle scaffolds (silica, gold, micelles, polymers, and semiconducting quantum dots) which are subsequently doped with DTPA, DOTA, or derivatives [20–24]. This review focuses on the synthesis of Gd³⁺, Mn²⁺, Dy³⁺, and Ho³⁺ nanoparticles and their biomedical application as contrast agents for MRI and multimodal imaging.

2. Paramagnetic Ion-Based Relaxivity

The relaxivity value, in mM⁻¹·s⁻¹, quantifies the ability of a contrast agent to promote contrast in MRI. In paramagnetic ions, two different mechanisms should be considered, both an inner-sphere and an outer-sphere mechanism, respectively:

The inner-sphere mechanism is predominant in paramagnetic contrast agents. It represents the influence of the paramagnetic ion on highly local water protons and is sensitive to the chemical water exchange between the first coordination sphere (inner-sphere) of the paramagnetic ion and bulk water. In terms of longitudinal relaxation rate, a range of different factors are considered within the inner-sphere contribution as described in the following equation:

where Pm refers to the mole fraction of the paramagnetic ion, q is the number of water molecules coordinated to the metal centre (hydration number), T1m the relaxation time of the water protons bounded to the ion, and τM is the bound residence lifetime. By considering equation (2) and Solomon–Bloembergen–Morgan (SBM) theory, three important parameters must be taken into consideration in agent design: hydration number (q), residence lifetime (τM), and the rotational correlation time (τR) [25]. The hydration number is usually equal to 1 in kinetically stable lanthanide complexes though a broad range of chelate agents with larger hydration numbers (and higher longitudinal relaxivities) have also been reported [26, 27]. An increase in hydration can come with the cost of stability (either chemical or signal output) [4]. Residence lifetimes should scale inversely with strength of the magnetic field and optimal values have been found to be in the range of 1–30 ns [28]. In addition, it has been shown that τR governs the relaxivity when τM is in the optimal range. The best relaxometric performance is typically observed when τR is the range of a few nanoseconds [29].

Gadolinium (Gd³⁺) and manganese (Mn²⁺) are the most used paramagnetic T₁ contrast agents for MRI. Gd³⁺ has 7 unpaired electrons in the 4f subshell and a high associated spin quantum number (S = 7/2). Mn²⁺ contains 5 unpaired electrons in its valence d orbitals and hence also a high spin quantum number (S = 5/2). Both of them present high magnetic moments, symmetric orbital ground states, large longitudinal electronic relaxation times (∼10⁻⁸ s), and fast water exchange kinetics [30, 31].

Although paramagnetic ions are more commonly used as T₁ contrast agents, there are examples, such as Dy³⁺ and Ho³⁺, which present notable T₂ contrast. In contrast to Mn and Gd, Dy³⁺ and Ho³⁺ have highly anisotropic ground states with substantial spin-orbit and Zeeman effects [32]. Therefore, these ions show very short electronic relaxation times (∼10⁻¹³ s) that are accompanied by high effective magnetic moment (μeff Dy³⁺ = 10.6 μB). Due to these properties, Dy³⁺ and Ho³⁺ coordination complexes are found to affect primarily the transversal relaxivity (T₂ contrast) with relaxivities increasing with magnetic field strength by a Curie relaxation mechanism. The latter points to facilitating an application of Dy³⁺/Ho³⁺ molecular complexes in ultrahigh field MRI [33, 34].

It has been reported that the inner-sphere contribution governs the transverse relaxation of bulk water in these complexes where slow water exchange results in better relaxivities [35, 36]. Very recently, it has been demonstrated that the incorporation of Dy-DOTA complexes into silica nanoparticles changes the relaxation mechanism towards that associated with an enhanced Curie outer-sphere contribution [20].

3. Paramagnetic Nanoparticles

A very broad range of nanoparticulate MRI contrast agents have been reported. This review will focus on a few of these (Table 1), namely, those associated with the paramagnetic ions Gd³⁺, Mn²⁺, Dy³⁺, and Ho³⁺, since they have been extensively studied and often show striking MRI performance.

Table 1: Examples of nanoparticle-based paramagnetic contrast agents for magnetic resonance imaging included in this review.

Material
Size (nm)
r₁ (mM⁻¹·s⁻¹)
r₂ (mM⁻¹·s⁻¹)
Magnetic field (T)
Reference

Gd-Si-DTTA
37
19.7
60.0
3
[37]

MSN-DTTA-Gd
75
28.8
65.5
3
[38]

Gd-DOTA-MSNs
66.3 ± 6.6
33.6 ± 1.3
—
7
[39]

CeOx : Gd9%
3.8
13.4
25.8
1.41
[40]

GO-DOTA-Gd
20–50
14.2
—
11.7
[41]

iGd-TMV-Si
300 × 18 nm, 4 nm internal channel
29.7
—
1.41
[42]

Gd-DND
4.9
33.4
332.0
8
[43]

Hsp DTPA-Gd nanocage
37.1
47.4
—
1.5
[44]

DOTA(Gd)-Fe@Fe₃O₄
258
7.2
109.4
0.5
[45]

Gd-M-dots
14.58
23.4
123.3
3
[46]

Gd₂O₃
1.0
9.9
10.5
1.5
[15]

Silica-Gd₂O₃
91.5
30.8
—
0.55
[47]

Gd₂O₃ : Eu³⁺
7.4 ± 0.3
34.3
—
3
[48]

QDs@DTDTPA-Gd
24.7 ± 2.7
9.9
—
3
[49]

A-C-dots@Ce6
98 ± 10
32.1
—
3
[50]

GBCAs-BP
70
15.4
73.5
3
[51]

GDO(Gd + Dy)
1.0
6.0
40.0
1.5
[52]

PEG-MnO
1.9
12.9
60.3
3
[53]

MnO
7
0.4
1.7
3
[16]

Mn₃O₄@CF
̴70
3.5
—
1.41
[17]

MnIO
17.3
57.8 ± 6.5
306.3 ± 15.2
0.5
[54]

OA-PL-HMON
—
1.1
9.2
3
[55]

NCP@peg-AA
78.6 ± 5.4
11.6
19.7
3
[56]

NPs-dopa-PEG-DOTA/RGD
26.4 ± 7.5
—
267.5
7
[57]

⁶⁴Cu-NOTA-FA-FI-PEG-PEI-Ac-Mn₃O₄
476.5 ± 13.5
1.0
—
0.5
[58]

Mn-LDH
48.0 ± 1.8
9.5 at pH 5.0
1.2 at pH 7.4
—
16.4
[59]

Fe₃O₄@C@MnO₂
150
5.3 at pH 5
2.2 at pH 7.4
364.2 at pH 5
442.4 at pH 7.4
3
[60]

MnO@Au NCs
45.0 ± 5.1
2.4 at pH 5.4
1.2 at pH 7.4
—
7
[61]

Dy-MSNs-L
166.2 ± 1.9
—
143.5 ± 8.2
11.7
[20]

Dy(DOTA)-Cy7.5-TMV-PEG-DGEA
300 × 18 nm, 4 nm internal channel
—
399.0
9.4
[62]

DyF₃
100.35
0.9
380.4
9.4
[63]

D₂O₃ (D-glucuronic acid coating)
3.2
0.008
65.0
1.5
[18]

NaDyF₄
20.3
0.3
101.0
9.4
[19]

Dy-doped MnCO₃
9.27 ± 0.72
4.5
—
7
[64]

Dy₂O₃ : Tb³⁺
3.0 ± 0.3
—
2.2
7
[65]

PEG-NaGdF₄:Dy
—
5.2
10.6
9.4
[66]

SiO₂@Gd₂O₃ : Dy³⁺
101.5
30.2
—
0.55
[67]

NaHoF₄
28.9
0.6
222.6
7
[68]

PEG-Ho₂O₃
80–90
—
23.5
1.5
[69]

HoF₃
94.3
0.6
608.4
9.4
[63]

3.1. Gadolinium

A broad range of approaches have been applied to the incorporation of Gd chelates into nanoparticles, some of which are included here, since they enable high levels of chemical tailoring [70]. Of them, gadolinium-doped silica nanoparticles have been extensively reported. A study by Rieter et al., for example, utilised a luminescent [Ru(bpy)₃]Cl₃ core to which a coating of a silylated Gd complex was applied [37]. This produced stable particles with r₁ values markedly greater than conventional molecular Gd chelates. This work was then taken further to produce mesoporous silica nanoparticles (MSNs) with even higher relaxivity values in an approach that has subsequently been adopted by many research groups [38]. Other work has demonstrated that the location of the Gd chelate within the MSNs greatly influences its relaxometric properties. The highest relaxivities have been specifically reported to occur when synthesis is by a long delay co-condensation process with r₁ value of 33.6 ± 1.3 mM⁻¹·s⁻¹, higher than any previously reported Gd-DOTA silica nanoparticles and 20 times larger than free Gd-DOTA (Figure 1) [39]. These particles were then biotinylated showing that the large relaxivity of the particles was essentially unchanged on external biomodification but was then reversibly gateable on subsequent protein recognition [72].

Figure 1: Typical transmission electron microscope image and schematic representation of Gd-DOTA-MSNs (66.3 ± 6.6 nm) prepared using (a) “short delay” co-condensation, where functionalities are internalised deeply in the structure (r₁ = 17.14 ± 0.49 mM⁻¹·s⁻¹), (b) “long delay” co-condensation, where functionalities are internalised nearer to the porous openings (r₁ = 33.57 ± 1.29 mM⁻¹·s⁻¹), and (c) postgrafting, where functionalities are loaded on external surfaces (r₁ = 10.77 ± 0.22 mM⁻¹·s⁻¹). (d) Postgrafted Gd-DOTA-non-porous silica nanoparticles (r₁ = 19.56 ± 0.47 mM⁻¹·s⁻¹) (reproduced from [71]).

Graphene oxide (GO) has also been used as a scaffold to integrate Gd-DOTA moieties. In one study, GO was first pegylated, functionalised with DOTA, and then metallated with Gd³⁺. These nanoparticles presented a large r₁ value of 14.2 mM⁻¹·s⁻¹ measured at 11.7 T [41]. Another interesting scaffold for Gd-DOTA is the use of the tobacco mosaic virus (TMV). TMV is a rod-like plant virus formed by 2130 identical coat proteins assembled into a 300 × 18 nm hollow tube with a 4 nm pore channel. Gd-DOTA was loaded into the interior channel by a copper-catalysed azide-alkyne cycloaddition reaction yielding r₁ = 10.9 mM⁻¹·s⁻¹. Surprisingly, this relaxivity increased to 29.7 mM⁻¹·s⁻¹ when the surface of the TMV was functionalised with silica [42].

In recent work, cerium oxide nanoparticles have been utilised to produce Gd-cerium nanoparticles with antioxidant capabilities. These nanoparticles, between 3 and 5 nm in size, rendered longitudinal relaxivities around 7–13 mM⁻¹·s⁻¹ [40]. Gd³⁺-impregnated nanodiamonds have also been used to produce CAs. These particles have been synthesised via an unusual detonation synthesis where an oxygen deficient trinitrotoluene/1,3,5-trinitroperhydro-1,3,5-triazine (TNT/RDX) mixture is detonated under an inert atmosphere. The nanodiamonds, formed in the early stages of the detonation under high pressure, reportedly are 4–5 nm in size with a narrow size distribution. These were then modified with Gd³⁺ at the surface carboxylic groups generating unprecedented transverse relaxivity (r₂ = 332 mM⁻¹·s⁻¹) and corresponding r₁ = 33.4 mM⁻¹·s⁻¹; the relaxivity mechanisms that are responsible for this high r₂ value warrant further analysis [43]. Another interesting example is a report of the synthesis of protein-based nanocages. These were further functionalised with maleimide-DTPA-Gd³⁺ using a cysteine residue of the protein with the relaxometric properties analysed for four nanocages with different sizes (from 16.8 to 37.1 nm). The data revealed that higher r₁ values (up to 47 mM⁻¹·s⁻¹ at 1.5 T) were obtained in larger nanocages due to the reduction in the tumbling rate of associated water molecules [44].

Gadolinium oxides are the most utilised alternatives to Gd chelates, where it has been found that decreasing particle diameter results in a progressive trend towards higher relaxivities. For instance, Park et al. showed that the highest relaxivities were obtained for nanoparticles synthesised with an average diameter of d = 1–2.5 nm [15]. It should be noted, however, that ultrasmall Gd₂O₃ have been found to form deposits in the brain and consequently there is a compromise between limiting the toxicity of the particles while maximising imaging potency. A study by Yin et al. produced silica nanoparticles coated in a Gd₂O₃ nanoshell of varying thicknesses. By systematically changing the thickness of the silica shell, the variations in relaxivity values could be investigated and demonstrated that a thinner shell resulted in larger r₁ values [47].

A broad number of dual modal paramagnetic particle systems have been developed [73]. Gd₂O₃ nanoparticles for fluorescence/MR imaging have, for example, been reported and doped with Eu³⁺ to produce Gd₂O₃ : Eu³⁺ particles. Fluorescence imaging here mediated by Gd³⁺ absorbing a photon and moving to a ⁶IJ excited state. Energy transfer between this state to the highly unstable ⁵D₀ state of Eu³⁺ is accompanied by the emission of a photon on which the electrons return to the ground state of Eu³⁺. This coupled with a high r₁ = 34.3 mM⁻¹·s⁻¹ results in an efficient bimodal imaging probe (Figure 2) [48]. CuInS₂/ZnS quantum dots have also been reported as good candidates for fluorescence/MRI bimodal imaging. These QDs were conjugated with a derivative compound of DTPA for further chelation with Gd³⁺. The composition of these QDs enhanced near-infrared fluorescence (NIRF) and MR imaging with a moderate r₁ = 9.91 mM⁻¹·s⁻¹ [49]. A recent approach has described the use of carbon dots (CDs) decorated with Gd³⁺ for fluorescence/MRI. In this work, CDs with surface carboxylic groups were obtained via a microwave synthesis. Gd³⁺ was then used to mediate a spherical assembly of the CDs by intercluster electrostatic linkages (-COO⁻-Gd³⁺-⁻OOC). This formulation showed a fluorescence enhancement with increasing Gd³⁺ concentration and a high r₁ value of 32.1 mM⁻¹·s⁻¹ [50].

Figure 2: Figure illustrating the dual-modal imaging properties of Gd₂O₃ : Eu³⁺ nanoparticles (reprinted (adapted) with permission from [48]).

A dual MR/CT imaging agent based on surface functionalised bisphosphonate (BP) gadolinium oxide nanoparticles has been developed. Gd₂O₃ nanoparticles were synthesised via the polyol method and encapsulated into a mesoporous silica shell by addition of glycidyloxipropyl trimethoxysilane (GPTES). The final formulation presented an r₁ = 15.41 mM⁻¹·s⁻¹ with r₂/r₁ = 4.77 at 3 T [51].

In addition to contrast agents enabling multiple imaging modalities, dual T₁-T₂ contrast agents can provide enhanced MRI imaging capabilities. Though it is sequence dependent, T₂ agents are, in general, natively disadvantaged since negative contrast generated by the contrast agent can easily be confused with natural artefacts coming from calcification or internal bleeding [74]. One approach providing T₁-T₂ contrast reported by Tirusew et al. used mixed Gd-Dy oxide nanoparticles [52]. Both gadolinium and dysprosium have high magnetic moments with the former promoting a shortening of longitudinal relaxation and dysprosium promoting the transverse relaxation. In an alternative approach, iron oxide nanoparticles were used instead of dysprosium to enhance T₂ contrast. In recent work, for example, Gd-labelled Fe@Fe₃O₄ particles have been reported with relaxivity values of r₁ = 7.2 mM⁻¹·s⁻¹ and r₂ = 109.4 mM⁻¹·s⁻¹ at 0.5 T [45]. Another example utilises biologically inspired ultrasmall (<10 nm) melanin nanoparticles loaded with Gd-DOTA as a T₁-T₂ dual-modal contrast agent. One of the active components, melanin, is a biological pigment that is paramagnetic in nature and chelates metal ions very strongly. Its use has thus been proposed as a promising alternative to traditional iron oxide or silica-based particles [46].

3.2. Manganese

Manganese nanoparticles have been extensively researched as possible T₁ contrast agents with reduced toxicity (compared to that of gadolinium) but however suffer from low native r₁ relaxivities [53, 75]. Much effort has been invested in increasing relaxivity and biocompatibility through the use of derived nanoparticulate systems. For instance, MnO nanoparticles have been encapsulated in polyethylene glycol showing no significant toxicity [16]. More recently, PEG-functionalised Mn₃O₄ nanoparticles have been encapsulated in a mesoporous, biocompatible carbon framework, with the latter enabling the water access required while apparently reducing Mn cation loss [17]. Other works conducted by Zhao et al. substituted Fe²⁺ ions on the surface of magnetite particles with Mn²⁺ to produce particles with T₁-weighted imaging capabilities [54]. On increasing the extent of doping of Mn²⁺ on the surface, an element with both a longer electronic relaxation time and greater paramagnetism than Fe²⁺, there was a corresponding increase in r₁.

Hollow Mn₃O₄ nanoparticles have been used in a study of the effect of surface functionalisation on relaxometric properties. It was observed, for example, that, with carboxylic acid functionalised ligands, there was a corresponding increase in r₁. This was proposed to be due to the induction of ferromagnetic spins between free surface spins [55]. Coordination polymers (NCP) of nanometric size (78.6 ± 5.4 nm) have been loaded with Mn²⁺ to produce efficient T₁ contrast agents. In this case, organic bridging ligands are used to produce a self-assembly process with Mn²⁺. These nanoparticles displayed high Mn loading of up to 13.3 ± 4 wt.% with a maximum r₁ value of 11.6 mM⁻¹·s⁻¹. The increase in the relaxivity was assigned to a reduction in the tumbling rates [56].

There has been a growing interest in the development of environmentally responsive MR agents [76]. One major class of these are pH responsive contrast agents which offer potential value in resolving the low pH microenvironment associated with tumour tissue [76]. Manganese-based double-layered hydroxide nanoparticles were the first reported ultrasensitive pH-responsive Mn-based contrast agent for T₁ MRI imaging, showing a 6-fold increase in longitudinal relaxivity values in acidic media (pH = 5) than at pH = 7.4, a switch assigned to the unique structure of Mn ions in the double-layered hydroxide [59]. Another reported pH responsive nanoparticulate example has been the synthesis of Fe₃O₄@C@MnO₂ nanoparticles. Here, iron oxide nanoparticles were coated with a carbon layer and reacted with KMnO₄ to produce MnO₂ nanosheets on the outer carbon shells. In a protic environment, these MnO₂ nanosheets are reduced to Mn²⁺ The increased concentration of paramagnetic Mn²⁺ ions in solution is accompanied by a corresponding increase in T₁ relaxation [60]. Manganese monoxide nanocomposites functionalised with porous gold nanoclusters have also been used as pH-responsive probes. In this work, it was suggested that the gold nanoclusters sterically hinder the release Mn²⁺ from the particles, consequently providing delayed T₁ contrast and a longer diagnostic window. They also allow the system to function as a multimodal probe with photoacoustic and X-ray CT imaging modalities additionally supported [61].

3.3. Dysprosium

Dysprosium is one possible alternative to conventional gadolinium-based contrast agents and acts as an effective T₂ contrast agent through its high magnetic moment (the largest of the lanthanides) and short (∼10⁻¹³ s) electronic relaxation time. Many approaches have been employed to produce dysprosium-modified nanoparticles, most recently MSNs that incorporate Dy-DOTA chelates in the outer pore channel producing particles with high r₂ values of 143.5 ± 8.2 mM⁻¹·s⁻¹ at 11.7 T, some 20 times larger in magnitude than the molecular analogue [20]. Even higher T₂ relaxivities have been reported by loading Dy-DOTA chelates into the cavity of the tobacco mosaic virus (r₂ values of 326 mM⁻¹·s⁻¹ at 7 T and 399 mM⁻¹·s⁻¹ at 9 T) [62]. Dysprosium oxide nanoparticles and dysprosium fluoride have also been proposed as promising agents. For example, González-Mancebo et al. produced DyF₃ rhombus-shaped nanoparticles with an average size of 110 × 50 nm. These nanoparticles present a remarkable r₂ of 380.4 mM⁻¹·s⁻¹ when measured at 9.4 T, ascribed to strong outer-sphere effects where the diffusion correlation time (τD) is affected by the effective radius of the nanoparticles [63]. Dysprosium oxide nanoparticles and dysprosium hydroxide nanorods have also been described as good candidates for T₂ contrast agents. Research by Kattel et al. synthesised d-glucuronic acid-coated ultrasmall Dy₂O₃ nanoparticles with an average size of 3.2 nm and Dy(OH)₃ nanorods with an average size of 20 × 300 nm. The first formulation showed an r₂ = 65.0 mM⁻¹·s⁻¹ whilst the nanorods possessed a higher value (r₂ = 181.57 mM⁻¹·s⁻¹ at 1.5 T) due to their greater size [18]. β-NaDyF₄ nanoparticles have shown potential as ultrahigh field magnetic resonance imaging (9.4 T) agents with high r₂ values [19]. Another formulation based on the synthesis of MnCO₃ nanoparticles doped with Dy exhibited reported r₁ values higher than those typical of MnO NPs [64].

There are numerous reports of dual-modal contrast nanoparticulate agents containing dysprosium. Tb³⁺-doped Dy₂O₃ nanoparticles have, for example, been reported to possess both MRI and optical imaging modalities since Tb³⁺ emits across the range of 489 nm to 619 nm, the brightest region being ∼545 nm (green) [65]. PEGylated NaGdF₄ : Dy nanoprobes as both dual-modal T₁-T₂ and MRI/CT agents were produced by Jin et al. T₁-weighted contrast is provided by the presence of Gd³⁺ ions with T₂-weighted contrast promoted by the doped Dy³⁺ ions, which also facilitates the use of the nanoprobe for CT imaging [66]. Dysprosium has been used to dope Gd₂O₃ nanoparticles resulting in dual-modal MR/fluorescence imaging [67]. These particles contain a silica core encapsulated by a Gd₂O₃ : Dy³⁺ nanoshell. Altering the thickness of this shell changed the relaxometric properties of the system with the highest r₁ values being reported for the thinnest (2 nm) shells.

3.4. Holmium

Holmium is another paramagnetic lanthanide with a highly effective magnetic moment and short electronic relaxation time. The first example of single Ho³⁺-doped upconversion nanoparticles for T₂-weighted MRI was reported by Ni et al., focusing on Ho³⁺-doped NaYbF₄ with surface phospholipid-PEGylation. Incorporation of both Yb³⁺ as a sensitizer and Ho³⁺ as an activator facilitates upconversion such that optical emission in the visible region is possible. In conjunction with efficient r₂ relaxation, this example is an effective dual-modal contrast agent [77]. NaHoF₄ nanoparticles have also been shown to be effective for T₂-weighted MRI with reported values of r₂ = 222.6 mM⁻¹·s⁻¹ at 7 T [68], as have holmium oxide nanoparticles. [69] The highest reported r₂ values for holmium, and indeed any of the examples mentioned in this review, are for rhombus-like HoF₃ nanoparticles produced by González-Mancebo et al. with r₂ = 608.4 at 9.4 T.

In this work, two different HoF₃ formulations were synthesised by homogeneous precipitation in ethylene glycol. Particle size and shape was notably tuneable with Ho(NO₃)₃ rendering ellipsoid-like nanoparticles (so-called HoF-el) and 70 × 30 nm in size (Figures 3(a) and 3(b)). An alternative with Ho(CH₃CO₂)₃ as the precursor formed rhombus-like nanoparticles (so-called HoF-rh) with an average size of 110 × 50 nm (Figures 3(c) and 3(d)). Although HoF-el displayed large r₂ value of 350.0 mM⁻¹·s⁻¹ at high magnetic fields (9.4 T), an unprecedented r₂ value of 608.4 mM⁻¹·s⁻¹ was reported for HoF-rh indicating not only a size dependency (increased magnetisation of the larger particles) but also pronounced geometric effects [63].

(a)

(b)

(c)

(d)

Figure 3: TEM images and size distribution plots obtained from TEM images of (a, b) HoF-el and (c, d) HoF-rh NPs (reprinted (adapted) with permission from [63]).

4. Multimodal Imaging and Theranostic Applications

Paramagnetic nanoparticles have applications in responsive MRI, targeted imaging, cell tracking, multimodal imaging, and as part of a theranostic platform. Here, some relevant and interesting examples reported across the last few years are described.

Very recently, Gd³⁺ complexes have been integrated into protein, calcium phosphate, polymeric, gold, and bismuth based nanoparticles. These have been utilised as nanotheranostics tools for multimodal imaging and in cancer therapy but also for the chemical imaging of neurotransmitters [78–83]. In 2018, Gd₂O₃ nanoparticles were applied to the targeted imaging of integrins for cancer diagnostics, cell labelling studies, and the multimodal imaging of calcium phosphate bone cement [51, 84, 85]. Gd³⁺ has been immobilized into metallofullerenes for MRI and photothermal therapy at tumour sites, within cerium oxide nanoparticles (CeNP) as a promising antioxidant theranostic agent, within leukosomes with enhanced activity towards activated endothelium cells, and on carbon dots for imaging-guided radiotherapy of tumours [40, 86–88].

Liu et al. reported multifunctional redox/pH responsive MnO₂ nanoparticles for cancer theranostics [89]. Based on honeycomb MnO₂ nanoparticles (hMnO₂), Sun et al. have developed pH/H₂O₂ responsive nanoparticles loaded with the photosensitiser chlorin e6 (Ce6). These upconverting nanoparticles, denoted as hMUC (Figure 4(a)), support high in vivo MRI T₁ contrast within tumours (Figure 4(b)). The presence of high Z-elements also facilitated CT imaging (Figure 4(c)). Finally, the particles were able to produce reactive oxygen species (ROS) through action of the Ce6 photosensitiser enabling tumour treatment by photodynamic therapy (PDT) [90].

(a)

(b)

(c)

Figure 4: (a) Schematic illustration for the synthesis of UCNPs-Ce6 and hMUC. (b) In vivo T₁-weighted MR images of a tumour-bearing mouse before (left) and after (right) intravenous injection of hMUC. (c) In vivo CT images of a tumour-bearing mouse before (upper) and after (lower) intratumour injection (reprinted (adapted) with permission from [90]).

Similar methodologies have been reported in enabling the pH and/or redox responsive diagnosis and photodynamic therapy of tumours using MnO₂ nanoparticles coupled with gold nanocages, copper sulfide nanostructures, or iron oxide nanoparticles [91–94].

Wang et al. have recently reported the synthesis of holmium-doped hollow silica nanospheres to create multifunctional theranostic nanoparticles. These were subsequently conjugated with a prostate stem cell antigen (PSCA) monoclonal antibody for targeted bimodal US/MRI of tumours as well as combined sonodynamic and hypoxia activated therapy [95]. Another application of multifunctional nanoparticles for dual-modal imaging is reported by Li et al., where ternary-doped (fluorine, ytterbium, and holmium) hydroxyapatite nanoparticles were used for multimodal imaging/tracking of hydroxyapatite in hard tissue repair [96]. Pegylated NaHoF₄ nanoparticles have also been employed for single MR and MR/CT dual-modality imaging applications [68, 97].

A notable example is reported by Hu et al. where dysprosium-modified tobacco mosaic virus nanoparticles were used for the bimodal MR/NIRF imaging of prostate cancer. In this work, internal glutamic acid residues were exploited to functionalise the nanoparticles with Dy-DOTA-azide and a NIRF dye Cy7.5-azide by a copper-catalysed azide-alkyne click chemistry cycloaddition (Figure 5(a)). Then, the external surface was conjugated with a DGEA peptide that binds to a specific integrin on the surface of PC-3 prostate cancer cells. The final formulation was able to produce targeted NIRF imaging in nude mice (Figure 5(b)), displaying strong T₂ contrast as supported by a high transverse relaxivity (399 mM⁻¹·s⁻¹ at 9.4 T) (Figure 5(c)) [62].

(a)

(b)

(c)

Figure 5: (a) Structure of the tobacco mosaic virus (TMV) nanoparticle’s coat protein with surface-exposed residues highlighted as internal glutamic acid (blue) and external tyrosine (red) and the structure of the assembled capsid/strategy for internal modification. (b) Near-infrared fluorescence (NIRF) imaging of subcutaneous PC-3 (α2β1) prostate tumours in athymic nude mice (n = 3) before and 1, 6, and 24 h after the intravenous injection of Dy-Cy7.5-TMV-mPEG (control group) or Dy-Cy7.5-TMV-DGEA (targeting group). (c) In vivo T₂-mapping MRI of subcutaneous PC-3 (α2β1) prostate tumours in athymic nude mice (n = 3) obtained before and 1, 6, and 24 h after the intravenous injection of Dy-Cy7.5-TMV-mPEG (control group) and Dy-Cy7.5-TMV-DGEA (targeting group) (reprinted (adapted) with permission from [62]).

5. Conclusions

Paramagnetic contrast agents present themselves as valuable tools for a broad range of potent MRI applications. Although traditional paramagnetic coordination complexes have been extensively applied, control over both thermodynamic and kinetic stability, pharmacokinetics, biodistribution, and imaging potency is limited. There are a wide variety of accessible synthetic procedures to develop nanoparticles conjugated with paramagnetic ions with a control over composition, size, and shape. This supports facile management of stability, pharmacokinetics, and biodistribution. Associated paramagnet hydration, water exchange kinetics, or residence lifetime can be tuned, all at high levels of local loading. The ability to readily integrate additional imaging modes and the employment of multivalent vectors across comparatively high particle surface areas make these yet more promising. To date, these constructs have been applied to the targeted multimodal molecular imaging, of cancer, cardiovascular, and neurological diseases as well as drug delivery, photodynamic, and sonodynamic therapy. One would fully expect the chemical richness available here to be truly impactful over the next decade.

Conflicts of Interest

The authors declare that they have no conflicts of interest.

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