Case Reports in Hematology
Volume 2019 (2019), Article ID 1430170, 4 pages
https://doi.org/10.1155/2019/1430170

Case Report

Homozygous CALR Mutation in Primary Myelofibrosis and Its Effect on Disease Phenotype: A Case Report and Review of the Literature

Qurratulain Rizvi,¹ Uzma Zaidi,¹ Saba Shahid,² Shariq Ahmed,² and Tahir Shamsi¹

¹Department of Clinical Haematology, National Institute of Blood Diseases & Bone Marrow Transplantation, P.O. Box-75300, Karachi, Pakistan
²Department of Genomics, National Institute of Blood Diseases & Bone Marrow Transplantation, P.O. Box-75300, Karachi, Pakistan

Correspondence should be addressed to Saba Shahid; sabashahid_dbt@yahoo.com

Received 14 September 2018; Revised 25 November 2018; Accepted 18 December 2018; Published 20 January 2019

Academic Editor: Eduardo Arellano-Rodrigo

Copyright © 2019 Qurratulain Rizvi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract.

Somatic mutations in CALR gene have been reported in 60%–88% of patients with essential thrombocythemia (ET) and primary myelofibrosis (PMF) who are negative for JAK2 and MPL mutations. Most of the CALR mutations analyzed to date are heterozygous mutations in exon 9 of the gene. Homozygosity in CALR gene is rarely reported, and its association with clinical behavior of disease and impact on outcome of patients is not studied so far. We herein report a case of intermediate-2 risk PMF (according to IPSS) diagnosed with homozygous mutation (c.1139delA p.E380fs50) in CALR gene having severe disease manifestations at presentation.

1. Introduction

Primary myelofibrosis (PMF) is a classical Philadelphia-negative myeloproliferative neoplasm (MPN) characterized by abnormal proliferation of predominantly megakaryocytes and granulocytes. The disease involves remodeling of bone marrow, including progressive myelofibrosis, exaggerated angiogenesis, and extramedullary hematopoiesis manifested by leukoerythroblastic blood smear and hepatosplenomegaly [1]. A number of molecular pathways and mutations play role in the pathogenesis of the disease making it a complex disorder.

Approximately, 50% of patients with PMF carry the JAK2‐V617F mutation, whereas mutations of MPL are found in an additional 5%. Somatic mutations in CALR gene were detected in 70% to 84% of JAK2 nonmutated MPN [2]. An entity defined as triple-negative primary myelofibrosis with absent JAK2, CALR, and cMPL mutation is identified, being associated with reduced overall survival [3]. Most of the CALR mutations analyzed to date are heterozygous mutations in exon 9 of the gene. Patients with heterozygous CALR-mutant PMF have distinct clinical features, an indolent clinical course, and better survival compared with PMF patients carrying JAK2 (V617F) or an MPL exon 10 mutation [4, 5]. Due to the high prevalence of CALR mutation in PMF, WHO has incorporated its presence in the revised diagnostic criteria of prefibrotic and overt myelofibrosis [6]. Homozygosity in CALR gene is rarely reported, and its association with clinical behavior of disease and impact on outcome of patients is not studied so far [7]. We herein report a case of PMF diagnosed with homozygous mutation in CALR gene having severe disease manifestations at presentation.

2. Case Scenario

A 57-year-old gentleman, known diabetic, presented with complaints of easy fatigability, loss of appetite, low-grade fever, and left hypochondrial discomfort for the past 2 months. Physical examination revealed splenomegaly (8 fingers below the left costal margin). Complete blood count showed Hb of 9.4 g/dl, TLC of 5.8 × 10⁹/L, and Plt count of 337 × 10⁹/L with leukoerythroblastic picture and absent circulating blasts. Bone marrow findings were consistent with primary myelofibrosis, exhibiting hypercellularity with marked increase in reticulin fibrosis (MF grade 2, according to EUMNET consensus, Figure 1) and atypical, hypolobated megakaryocytes forming loose clusters (Figure 2). Blast percentage was less than 5%. Cytogenetic analysis showed normal male karyotype. BCR-ABL translocation via PCR, JAK2‐V617F, and MPL mutation were not detected. Associated CALR mutations were not determined via next-generation sequencing due to resource constraints. Serum erythropoietin level was 200 U/L. According to the International Prognostic Scoring System (IPSS), the patient was falling into intermediate-2 risk category. He was initially prescribed ruxolitinib 10 mg twice daily (gradually escalated to 15 mg twice daily) along with erythropoietin 20,000 IU weekly for correction of anemia.

Figure 1: Reticulin stain showing MF grade 2 fibrosis.

Figure 2: Trephine biopsy showing atypical megakaryocytes.

3. Methodology

The research protocol was approved by the Institutional Review Board (ERC/IRB) and conformed to the tenets of the Declaration of Helsinki. Written informed consent was obtained from the patient. The diagnosis was established according to the 2016 WHO criteria, and the clinical and laboratory data were reviewed from medical record.

3.1. Mutation Analysis

The DNA was extracted from the peripheral blood sample of the MPN patient using a QIAamp DNA Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. The JAK2 and MPL mutations were analyzed by using the ARMS PCR for JAK2‐V617F and MPL W515L/K mutations as previously described [8]. Sanger sequencing was performed to screen CALR gene for exon 9. The genomic region of interest was amplified by PCR. Amplification of CALR exon 9 was performed by using previously reported primers CALR. –F (5′-ACAACTTCCTCATCACCAACG-3′) and CALR–R (5′- GGCCTCAGTCCAGCCCTG -3′) [8]. The total reaction volume of 20 μl contained approximately 50 ng of DNA, 200 nmol/l for forward and reverse primers, deoxynucleotide triphosphates (dNTPs, 200 µmol/l each), 1 unit of Dream Taq DNA Polymerase (Thermo Scientific, Life Technologies Inc, Carlsbad, California 92008). Samples were amplified using the following PCR conditions: 94°C for 10 minutes; 30 cycles of 94°C for 30 seconds; 59°C for 30 seconds; 72°C for 30 seconds with final extension of 72°C for 10 minutes and hold at 4°C. PCR products were analyzed using 2% agarose gel electrophoresis. Sequencing products were purified, and the sequencing reaction was performed using the BigDye terminator cycle sequencing kit, v3.1 (Applied Biosystems®, California, USA) and was analyzed on an automated DNA analyzer (ABI, 3500).

4. Results and Discussion

Sanger sequencing for CALR exon 9 mutation identified a homozygous mutation (c.1139delA p.E380fs50) in this case as shown in Figure 3. The same mutation has been reported in Chinese population in heterozygous state which disturbed the reading frame shift and significantly altered C-terminal domain of CALR protein [9]. This is a unique finding, as homozygosity in CALR gene has been exclusively associated with type 2 mutation (5 bp insertion; 1). Basically, CALR mutations do not alter the primary structure of the CALR binding site for glycoproteins but affect the C-terminal domain, which contains a KDEL motif and a Ca²⁺ binding domain [10].

Figure 3: Electropherogram of CALR exon 9 homozygous mutation (c.1139delA; p.E380fs50) and the new peptide sequence.

The advent of mutation in CALR gene changed the landscape of MPNs. It was first recognized as a somatic mutation in patients with MPNs who had no mutations in either JAK2 or MPL by Klampfl et al. in 2013 [7]. Calreticulin is a protein found in endoplasmic reticulum, cytoplasm, or cell surface, which maintains the calcium hemostasis, regulates the cell proliferation, phagocytosis, and apoptosis, and also ensures proper glycoprotein folding [11]. Patients with heterozygous CALR-mutated PMF are usually males, younger as compared to JAK2-mutated cases, and they have myeloproliferation more specific to the megakaryocytic lineage, thus presenting with a more pronounced thrombocytosis. They usually have low hemoglobin and white cell counts. There is low incidence of thrombotic complications and longer survival reported in this group of patients. This prognostic impact of CALR in PMF is limited to type 1 mutation, with type 2 having similar prognosis as that of JAK2-mutated PMF [12].

Mutated CALR homozygosity seems to be a rare event in MPNs and is reported with type 2 mutations in exon 9. In the study by Klampfl et al., three of 289 CALR-mutated patients were homozygous for the mutation, and all had type 2 mutations [7]. Similar frequencies of acquired 19pUPD for both CALR insertions and deletions were found by Nangalia et al. [13]. There is not much literature to provide specific evidence concerning homozygous CALR mutations and its impact on the phenotypic behavior of the disease as well as on the type of CALR mutation.

Homozygous CALR mutation in PMF has been associated with acquired myeloperoxidase (MPO) deficiency as reported by Alexandre et al. [14]. An individual case report of homozygous CALR type 1 mutation has been described in a patient with an atypical BCR-ABL1-positive MPN [15].

Our index case was diagnosed in the fibrotic phase of PMF. He was found to be in intermediate-2 risk category according to IPSS. His molecular mutation profile was negative for JAK2, MPL, and BCR-ABL translocation. Interestingly, the mutation in CALR gene detected in this patient was homozygous mutation (c.1139delA p.E380fs50), which seems to be a type 2-like mutation based on the classification according to the absence of (KDEL) motif in the wild type and modification in the alpha helix structure. These mutations produce a new C-terminus which results in loss of endoplasm reticulum (ER) retention signals but maintain the structural integrity of protein. The resultant mutant CALR was found in ER without accumulation on the cell surface or Golgi apparatus [13]. This structural modification affects the function and leads to decrease in Ca⁺² export from the ER and causes downregulation of the calreticulin-NFAT signaling pathway which is directed more towards myeloid lineage commitment [16].

In contrast to the clinical findings associated with heterozygous mutations, i.e., lesser disease severity, thrombocytosis at baseline, and overall good prognosis, our patient with homozygous mutation presented in the advanced phase of disease with constitutional symptoms including weight loss, anorexia, fatigue and low-grade fever, and massive splenomegaly requiring treatment. His platelet count was within normal range at presentation. He was offered JAK2 inhibitor and erythropoiesis-stimulating agent. After a period of 6 months, the patient was reevaluated using dynamic IPSS (DIPSS) and fell into the intermediate-2 risk category. No thrombotic or hemorrhagic event was noted during the follow-up period. There was mild improvement in constitutional symptoms with ruxolitinib, but red cell transfusion requirement gradually increased. Though initial therapy with ruxolitinib is associated with drop in hemoglobin levels, the severe disease manifestations at presentation compels us to consider that homozygous mutations in CALR gene might be accountable for an aggressive disease phenotype in PMF.

5. Conclusion

As CALR mutation in MPNs is one of the recent advances, its extensive impact on baseline characteristics and clinical behavior of patient, disease outcome, and risks and benefits in long term is yet to be explored. Prospective studies are needed to elucidate the influence of CALR mutation in MPNs with extensive focus on the homozygous pattern of mutation.

Conflicts of Interest

The authors declare that they have no conflicts of interest.

References

A. Tefferi and A. Pardanani, “CALR mutations and a new diagnostic algorithm for MPN,” Nature Reviews Clinical Oncology, vol. 11, no. 3, pp. 125-126, 2014.

M. Cazzola and R. Kralovics, “From Janus kinase 2 to calreticulin: the clinically relevant genomic landscape of myeloproliferative neoplasms,” Blood, vol. 123, no. 24, pp. 3714–3719, 2014.

C. Diaconu, C. Mambet, L. G. Necula et al., “Triple negative myeloproliferative neoplasms - sometimes driver mutations stay low-key in plain sight,” Romanian Biotechnological Letters, vol. 10, 2017.

A. Tefferi, T. L. Lasho, C. M. Finke et al., “CALR vs JAK2 vs MPL-mutated or triple-negative myelofibrosis: clinical, cytogenetic and molecular comparisons,” Leukemia, vol. 28, no. 7, pp. 1472–1477, 2014.

E. Rumi, D. Pietra, C. Pascutto et al., “Clinical effect of driver mutations of JAK2, CALR, or MPL in primary myelofibrosis,” Blood, vol. 124, no. 7, pp. 1062–1069, 2014.

A. Tefferi, “Primary myelofibrosis: 2017 update on diagnosis, risk-stratification, and management,” American Journal of Hematology, vol. 91, no. 12, pp. 1262–1271, 2016.

T. Klampfl, H. Gisslinger, A. S. Harutyunyan et al., “Somatic mutations of calreticulin in myeloproliferative neoplasms,” New England Journal of Medicine, vol. 369, no. 25, pp. 2379–2390, 2013.

Z. Wu, Y. Zhang, X. Zhang et al., “A multiplex snapback primer system for the enrichment and detection of JAK2 V617F and MPL W515L/K mutations in Philadelphia- negative myeloproliferative neoplasms,” BioMed Research International, vol. 2014, Article ID 458457, 11 pages, 2014.

Y. Lin, E. Liu, Q. Sun et al., “The prevalence of JAK2, MPL, and CALR Mutations in Chinese patients WithBCR-ABL1-negative myeloproliferative neoplasms,” American Journal of Clinical Pathology, vol. 144, no. 1, pp. 165–171, 2015.

J. Chi, M. Manoloukos, C. Pierides et al., “Calreticulin mutations in myeloproliferative neoplasms and new methodology for their detection and monitoring,” Annals of Hematology, vol. 94, no. 3, pp. 399–408, 2014.

W. Luo and Z. Yu, “Calreticulin (CALR) mutation in myeloproliferative neoplasms (MPNs),” Stem Cell Investig, vol. 2, no. 16, 16 pages, 2015.

P. Guglielmelli, J. Nangalia, A. R. Green, and A. M. Vannucchi, “CALRmutations in myeloproliferative neoplasms: hidden behind the reticulum,” American Journal of Hematology, vol. 89, no. 5, pp. 453–456, 2014.

J. Nangalia, C. E. Massie, E. J. Baxter et al., “Somatic CALR mutations in myeloproliferative neoplasms with nonmutated JAK2,” New England Journal of Medicine, vol. 369, no. 25, pp. 2391–2405, 2013.

P. T. Alexandre, L. Pontus, K. L. Asvin et al., “Homozygous calreticulin mutations in patients with myelofibrosis lead to acquired myeloperoxidase deficiency,” Blood, vol. 127, no. 25, pp. 3253–3259, 2016.

X. Cabagnols, J.-M. Cayuela, and W. Vainchenker, “A CALR mutation preceding BCR-ABL1 in an atypical myeloproliferative neoplasm,” New England Journal of Medicine, vol. 372, no. 7, pp. 688–690, 2015.

M. P. Chao, S. Jaiswal, R. Weissman-Tsukamoto et al., “Calreticulin is the dominant pro-phagocytic signal on multiple human cancers and is counterbalanced by CD47,” Science Translational Medicine, vol. 2, no. 63, p. 63ra94, 2010.