Case Reports in Infectious Diseases

Volume 2013 (2013), Article ID 589490, 4 pages

http://dx.doi.org/10.1155/2013/589490

Case Report

Combination Antifungal Therapy in the Treatment of Scedosporium apiospermum Central Nervous System Infections

Andrés F. Henao-Martínez, José R. Castillo-Mancilla, Michelle A. Barron, and Aran Cunningham Nichol

Division of Infectious Diseases, University of Colorado Denver, 12700 E. 19th Avenue, Mail Stop B168, Aurora, CO 80045, USA

Received 6 March 2013; Accepted 8 April 2013

Academic Editors: C. L. Gibert and D. L. Palazzi

Copyright © 2013 Andrés F. Henao-Martínez et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract.

Treatment of Scedosporium apiospermum central nervous system (CNS) infection typically consists of an azole in combination with surgical debridement. This approach requires prolonged treatment and carries a high associated mortality. We present two cases of the successful treatment of S. apiospermum CNS infections with the combination of voriconazole and terbinafine.

1. Case Reports

1.1. Case  1

An 81-year-old Hispanic woman with a past medical history significant for hypertension, diabetes mellitus, hypothyroidism, and chronic kidney disease presented with symptoms of sinusitis, including facial pain, nasal congestion, and rhinorrhea. The sinusitis was later complicated by the development of left orbital cellulitis. The patient underwent a functional endoscopic sinus surgery (FESS) without evidence of gross infection and was treated with oral levofloxacin. Three weeks later, her orbital cellulitis resolved; however, she developed nausea, vomiting, headaches, left vision loss, diplopia, and local pain. Her eye exam was significant for intact extraocular movements, no nystagmus, and a positive afferent pupillary defect. No periorbital edema or erythema was observed. Visual acuity for her right eye was 20/60 and for her left eye was 20/100. Her basic laboratory results were normal. She was diagnosed with a left orbital complex syndrome with optic neuropathy and underwent another FESS with sinusotomy and debridement. The patient was briefly placed on intravenous (IV) steroids, and she was discharged home on an oral prednisone taper and levofloxacin. However, the patient was readmitted 10 days later with worsening vision loss on the left; she was then only able to perceive light. The intraoperative cultures from her last FESS grew a moderate amount of mold, which was subsequently identified as Scedosporium apiospermum based on its characteristic macroscopic morphology and conidiation. Susceptibility testing of the isolate revealed a voriconazole MIC of 0.5 ug/mL. An orbital MRI revealed mildly elevated signal within the intraorbital segment of the left optic nerve, mild inflammatory changes surrounding the optic nerve sheath complex and the orbital apex, and very mild asymmetric prominence of the left extraocular muscles concerning for myositis (Figure 1). The patient was placed on IV voriconazole at 6 mg/kg every 12 hours for a presumed fungal sinusitis, myositis, and possibly sinus fungal osteomyelitis. After approximately 30 days of IV voriconazole therapy, the patient was transitioned to oral voriconazole and had obtained a voriconazole trough level of 2.06 ug/mL a month later. Two months after antifungal therapy was initiated, the patient presented with worsening headache, nausea, vomiting, and anorexia, and a repeat orbital MRI showed new mild left proptosis, enhancing soft tissue at the orbital apex extending along the cavernous sinus and under both frontal lobes along the dura, and slightly increased mucosal thickening throughout the sinuses with moderate to severe involvement of the left sphenoid and right maxillary sinus. The patient was evaluated for possible surgical debridement; however, due to the complexity of the disease location, it was decided to proceed with medical management only. Due to the rapid progression of her disease, renally dosed oral terbinafine was added at a dose of 250 mg daily. After 4 months of combination therapy, repeat orbital MRI showed stable inflammatory changes without progression. The patient’s clinical status eight months later is stable with no further CNS symptoms and good tolerance of her antifungal regimen.

Figure 1: Orbital MRI. Orbital MRI revealed mildly elevated signal within the intra-orbital segment of the left optic nerve, mild inflammatory change surrounding the optic nerve sheath and very mild asymmetric prominence of the left extra-ocular muscles concerning for myositis (arrows).

1.2. Case  2

A 64-year-old White man with a previous diagnosis of eosinophilic pneumonia, which had been treated with high-dose steroids, cyclophosphamide, and mycophenolic acid, presented with subacute symptoms of headaches, fevers, aphasia, and ataxia. He was diagnosed with meningitis, cerebritis, and ventriculitis based on an elevated cell count in his cerebral spinal fluid (CSF) and consistent imaging; however, the etiology was unclear. He was treated with antibacterial therapy, including a 10-day course of vancomycin, cefepime, and ampicillin, with partial resolution of his symptoms. He had a negative infectious workup including a negative brain biopsy, as well as, a normal cerebral angiogram. A CT scan of the head demonstrated hydrocephalus, and he had an external ventricular drain placed. A month later, his mental status worsened. At that time, his vital signs were normal and his neurologic exam was notable for mild confusion. Cranial nerves II through XII were intact with the exception of slow saccades, but no nystagmus was present. Laboratory studies were remarkable for a slightly elevated white blood cell count (WBC) of 12.2 × 10³/uL (normal range: 4.5–11.0 × 10³/uL) and a decreased hematocrit (Hct) of 29.1% (normal range: 42–54%). A repeat evaluation of his CSF showed 326 WBC (normal range: 0–5 × 10⁶/L) with 83% neutrophils (normal range: 0–6%). His CSF protein was 113 mg/dL (normal range: 15–45 mg/dL) and glucose was 54 mg/dL (normal range: 40–80 mg/dL). Gram stain and bacterial cultures were negative. An extensive infectious workup was negative including, CSF AFB smear and culture, serum and CSF cryptococcal antigen, Toxoplasma gondii antibody, Parvovirus B19 antibody, CSF VDRL, CSF and serum PCR for HHV-6, EBV, HSV-1/2, CMV, WNV, enterovirus, and JC virus. An MRI of his brain revealed ventriculomegaly, an increased halo surrounding the lateral ventricles, and debris within the ventricles with restricted diffusion and ependymal and subependymal enhancement (Figure 2). A repeat brain biopsy showed fungal-like elements (Figure 3). His CSF fungal cultures subsequently grew Scedosporium apiospermum. He was then started on oral voriconazole 600 mg twice daily and terbinafine 250 mg twice daily due to the severity of the infection. Two weeks later the voriconazole trough level was 2.58 ug/mL. His mental status improved substantially. Three months later, repeat CSF studies showed 3 WBC (normal range: 0–5 × 10⁶/L) with 3% neutrophils (normal range: 0–6%). His CSF protein was 42 mg/dL (normal range: 15–45 mg/dL) and glucose was 60 mg/dL (normal range: 40–80 mg/dL). Repeat CSF fungal cultures were negative. In light of his dramatic improvement, terbinafine was stopped and he continued on voriconazole only with regular monitoring of his drug level. Unfortunately, two months later the patient presented again with fevers and confusion, and his CSF cultures grew Scedosporium apiospermum. His clinical course was then complicated by influenza pneumonia, pulmonary embolism, ventricular drain malfunction, and renal failure. The patient failed to improve and ultimately was discharged to hospice and died one month later.

Figure 2: Brain MRI. Ventriculomegaly, increased halo surrounding the lateral ventricles, and debris within the ventricles with restricted diffusion and ependymal and subependymal enhancement (arrows).

Figure 3: H&E stain of the Brain Biopsy. Clusters of amorphous linear structures with a morphology simulating fungal hyphae.

2. Discussion

Scedosporium apiospermum, the anamorphic (asexual state) of Pseudallescheria boydii, is a ubiquitous organism with a worldwide distribution [1, 2]. This organism is most commonly associated with localized cutaneous and subcutaneous infections of the lower extremities after traumatic penetration with contaminated material (Madura foot) but can also cause disease at other sites such as the lung, brain, bone, eye, sinuses and joints [3–5]. Invasive and disseminated disease presenting with severe pneumonia, CNS infection, and endocarditis has been described in immunocompromised hosts, where the respiratory tract has been identified as the main major portal of entry [6–8]. In a review of 23 S. apiospermum infections in solid organ transplant recipients between 1976 and 1999, 8 patients developed dissemination, 11 had invasive lung disease, and 11 had CNS involvement, including 10 patients with brain abscesses [9]. In another series, almost 60% of 39 culture-proven cases of S. apiospermum infections of the CNS presented with an underlying immune impairment (immunosuppressive drugs, antineoplastic chemotherapy, or with a medical immune deficiency) [10]. In an Australian retrospective review of Scedosporium infections, S. apiospermum demonstrated invasiveness in 6% of cases, whereas S. prolificans was invasive in 36% of cases [11]. Regarding antifungal susceptibility, in vitro data suggest that the two antifungals with the best activity against S. apiospermum are voriconazole and micafungin [12]. Voriconazole has shown clinical efficacy and good tolerance in the management of Scedosporium spp. infections [13]. Measurements of voriconazole levels have been shown to be an effective method to improve efficacy and safety in this setting [14, 15]. Although in vitro data for terbinafine have shown high MICs against Scedosporium spp.; MICs in the literature are variable [16, 17], activity has been noted for some of the isolates [18, 19], and in vitro synergy between azoles and terbinafine has been reported for S. prolificans [17]. Likewise, terbinafine and voriconazole have been used successfully as combination therapy in the treatment of invasive S. apiospermum [20–23] and S. prolificans [24–26]. There is also a report of successful treatment of S. apiospermum with voriconazole in combination with caspofungin [27] or with posaconazole alone [28].

Predisposing factors for progressive fungal CNS infection in the first case we presented included advanced age, previous antibiotic and steroid exposure, the presence of diabetes mellitus and recent sinus instrumentation. As an alternative to terbinafine, an IV echinocandin could have been considered in combination with oral voriconazole in this case, but the likelihood of a prolonged, and possibly lifelong, treatment course made IV administration less feasible. The second patient had intense immunosuppressive therapy as his main predisposing factor for progressive fungal CNS infection. He clearly had a notable improvement with normalization of CSF cell counts and CSF sterilization while on combination antifungal therapy; however, due to his multiple comorbidities and a prolonged hospitalization, the infection relapsed and he died. Although successful reports of this antifungal combination have been described in the treatment of S. prolificans [24–26, 29], to our knowledge this is only the third case report of the successful use of the combination of voriconazole and terbinafine in the treatment of invasive CNS Scedosporium apiospermum infection. This treatment combination should be considered in cases of severe CNS infection with Scedosporium apiospermum, where surgical options are limited, and/or there has been a suboptimal response to voriconazole monotherapy.

Acknowledgments

No funding agencies had any role in the preparation, review, or approval of this paper. The views expressed in this paper are those of the authors and do not necessarily represent the views of the University of Colorado Denver. No conflict of interests was reported by Andrés F. Henao-Martínez, José R. Castillo-Mancilla, Michelle A. Barron, or Aran Cunningham Nichol.

References

L. Ajello, “The isolation of Aliescheria boydii shear, an etiologic agent of mycetomas, from soil,” The American Journal of Tropical Medicine and Hygiene, vol. 1, no. 2, pp. 227–238, 1952.

W. B. Cooke and P. Kabler, “Isolation of potentially pathogenic fungi from polluted water and sewage,” Public Health Reports, vol. 70, no. 7, pp. 689–694, 1955.

T. Miyamoto, R. Sasaoka, M. Kawaguchii et al., “Scedosporium apiospermum skin infection: a case report and review of the literature,” Journal of the American Academy of Dermatology, vol. 39, no. 3, pp. 498–500, 1998.

A. P. Grigg, P. Phillips, S. Durham, and J. D. Shepherd, “Recurrent pseudallescheria boydii sinusitis in acute leukemia,” Scandinavian Journal of Infectious Diseases, vol. 25, no. 2, pp. 263–267, 1993.

R. Tirado-Miranda, J. Solera-Santos, J. C. Brasero, M. Haro-Estarriol, P. Cascales-Sánchez, and J. B. Igualada, “Septic arthritis due to Scedosporium apiospermum: case report and review,” The Journal of Infection, vol. 43, no. 3, pp. 210–216, 2001.

T. J. Walsh, A. Groll, J. Hiemenz, R. Fleming, E. Roilides, and E. Anaissie, “Infections due to emerging and uncommon medically important fungal pathogens,” Clinical Microbiology and Infection, vol. 10, supplement 1, pp. 48–66, 2004.

A. Khurshid, V. T. Barnett, M. Sekosan, A. S. Ginzburg, and E. Önal, “Disseminated Pseudallescheria boydii infection in a nonimmunocompromised host,” Chest, vol. 116, no. 2, pp. 572–574, 1999.

T. A. O'Bryan, F. A. Browne, and J. F. Schonder, “Scedosporium apiospermum (Pseudallescheria boydii) endocarditis,” The Journal of Infection, vol. 44, no. 3, pp. 189–192, 2002.

B. Castiglioni, D. A. Sutton, M. G. Rinaldi, J. Fung, and S. Kusne, “Pseudallescheria boydii (Anamorph scedosporium apiospermum) infection in solid organ transplant recipients in a tertiary medical center and review of the literature,” Medicine, vol. 81, no. 5, pp. 333–348, 2002.

M. A. Nesky, E. C. McDougal, and J. E. Peacock Jr., “Pseudallescheria boydii brain abscess successfully treated with voriconazole and surgical drainage: case report and literature review of central nervous system pseudallescheriasis,” Clinical Infectious Diseases, vol. 31, no. 3, pp. 673–677, 2000.

L. Cooley, D. Spelman, K. Thursky, and M. Slavin, “Infection with Scedosporium apiospermum and S. prolificans, Australia,” Emerging Infectious Diseases, vol. 13, no. 8, pp. 1170–1177, 2007.

M. Lackner, G. S. de Hoog, P. E. Verweij, et al., “Species-specific antifungal susceptibility patterns of Scedosporium and Pseudallescheria species,” Antimicrobial Agents and Chemotherapy, vol. 56, pp. 2635–2642, 2012.

P. Troke, K. Aguirrebengoa, C. Arteaga et al., “Treatment of scedosporiosis with voriconazole: clinical experience with 107 patients,” Antimicrobial Agents and Chemotherapy, vol. 52, no. 5, pp. 1743–1750, 2008.

S. Miyakis, S. J. van Hal, J. Ray, and D. Marriott, “Voriconazole concentrations and outcome of invasive fungal infections,” Clinical Microbiology and Infection, vol. 16, no. 7, pp. 927–933, 2010.

A. Pascual, T. Calandra, S. Bolay, T. Buclin, J. Bille, and O. Marchetti, “Voriconazole therapeutic drug monitoring in patients with invasive mycoses improves efficacy and safety outcomes,” Clinical Infectious Diseases, vol. 46, no. 2, pp. 201–211, 2008.

F. Gilgado, C. Serena, J. Cano, J. Gené, and J. Guarro, “Antifungal susceptibilities of the species of the Pseudallescheria boydii complex,” Antimicrobial Agents and Chemotherapy, vol. 50, no. 12, pp. 4211–4213, 2006.

J. Meletiadis, J. W. Mouton, J. F. G. M. Meis, and P. E. Verweij, “In vitro drug interaction modeling of combinations of azoles with terbinafine against clinical Scedosporium prolificans isolates,” Antimicrobial Agents and Chemotherapy, vol. 47, no. 1, pp. 106–117, 2003.

J. Meletiadis, J. F. Meis, J. W. Mouton, et al., “In vitro activities of new and conventional antifungal agents against clinical Scedosporium isolates,” Antimicrobial Agents and Chemotherapy, vol. 46, no. 1, pp. 62–68, 2002.

G. Garcia-Effron, A. Gomez-Lopez, E. Mellado, A. Monzon, J. L. Rodriguez-Tudela, and M. Cuenca-Estrella, “In vitro activity of terbinafine against medically important non-dermatophyte species of filamentous fungi,” The Journal of Antimicrobial Chemotherapy, vol. 53, no. 6, pp. 1086–1089, 2004.

F. Morio, D. Horeau-Langlard, F. Gay-Andrieu et al., “Disseminated Scedosporium/Pseudallescheria infection after double-lung transplantation in patients with cystic fibrosis,” Journal of Clinical Microbiology, vol. 48, no. 5, pp. 1978–1982, 2010.

H. Sahi, R. K. Avery, O. A. Minai et al., “Scedosporium apiospermum (Pseudoallescheria boydii) infection in lung transplant recipients,” The Journal of Heart and Lung Transplantation, vol. 26, no. 4, pp. 350–356, 2007.

P. E. Verweij, N. J. M. Cox, and J. F. G. Meis, “Oral terbinafine for treatment of pulmonary Pseudallescheria boydii infection refractory to itraconazole therapy,” European Journal of Clinical Microbiology and Infectious Diseases, vol. 16, no. 1, pp. 26–28, 1997.

K. Mursch, S. Trnovec, H. Ratz et al., “Successful treatment of multiple Pseudallescheria boydii brain abscesses and ventriculitis/ependymitis in a 2-year-old child after a near-drowning episode,” Child's Nervous System, vol. 22, no. 2, pp. 189–192, 2006.

S. V. Bhat, D. L. Paterson, M. G. Rinaldi, and P. J. Veldkamp, “Scedosporium prolificans brain abscess in a patient with chronic granulomatous disease: successful combination therapy with voriconazole and terbinafine,” Scandinavian Journal of Infectious Diseases, vol. 39, no. 1, pp. 87–90, 2007.

I. B. Gosbell, V. Toumasatos, J. Yong, R. S. Kuo, D. H. Ellis, and R. C. Perrie, “Cure of orthopaedic infection with Scedosporium prolificans, using voriconazole plus terbinafine, without the need for radical surgery,” Mycoses, vol. 46, no. 5-6, pp. 233–236, 2003.

B. P. Howden, M. A. Slavin, A. P. Schwarer, and A. M. Mijch, “Successful control of disseminated Scedosporium prolificans infection with a combination of voriconazole and terbinafine,” European Journal of Clinical Microbiology and Infectious Diseases, vol. 22, no. 2, pp. 111–113, 2003.

S. Guignard, D. Hubert, B. Dupont et al., “Multifocal Scedosporium apiospermum spondylitis in a cystic fibrosis patient,” Journal of Cystic Fibrosis, vol. 7, no. 1, pp. 89–91, 2008.

I. K. Mellinghoff, D. J. Winston, G. Mukwaya, and G. J. Schiller, “Treatment of Scedosporium apiospermum brain abscesses with posaconazole,” Clinical Infectious Diseases, vol. 34, no. 12, pp. 1648–1650, 2002.

J. Y. Z. Li, T. Y. Yong, D. I. Grove, and P. T. H. Coates, “Successful control of Scedosporium prolificans septic arthritis and probable osteomyelitis without radical surgery in a long-term renal transplant recipient,” Transplant Infectious Disease, vol. 10, no. 1, pp. 63–65, 2008.