Cytological Features of a Variant NUT Midline Carcinoma of the Lung Harboring the NSD3-NUT Fusion Gene: A Case Report and Literature Review

Background. Nuclear protein in testis (NUT) midline carcinoma (NMC) is a very rare and aggressive malignancy. In more than two-thirds of these NMC cases, a fusion between NUT and BRD4 or BRD3 has been documented; other variants are rare. The cytology of NMC itself has been sparsely documented and that of variant NMC has never been reported. Case Presentation. A 36-year-old woman was admitted because of a rapidly progressing lung tumor with metastases to the breast and bone. We recently reported this patient as the first case of a variant NMC of the lung harboring an NSD3-NUT fusion, based on immunohistochemical and genetic analyses. Cytological material was available for the present review. A highly cellular smear contained a predominantly noncohesive pattern of monomorphic cells with diameters 2–2.5 times greater than those of small lymphocytes, with a round-to-oval nucleus, slightly irregular nuclear contours, variably prominent nucleoli, scant cytoplasm, and identifiable mitotic figures. Foci of stratification and overt pearl formation, including a dyskeratocyte, were occasionally observed. The necrotic background contained naked nuclei, karyorrhectic debris, apoptotic cells, and macrophages phagocytizing karyorrhectic debris; nuclear crushing was noted. Conclusion. The cytological features of a variant NMC of the lung are described for the first time.


Introduction
Nuclear protein in testis (NUT) midline carcinoma (NMC) is a recently recognized entity that is characterized by undifferentiated morphological features and immunoreactivity to NUT [1]. This disease is a very rare [2][3][4] and aggressive [3,4] malignancy that most often occurs in the midline of the body, including the head and neck and the mediastinum [2,4]. Currently, the diagnosis of NMC depends on the identification of a rearrangement involving the NUT locus at 15q14 that generates a specific fusion transcript with a member of the bromodomain-containing protein (BRD) family, such as BRD4 located on chromosome 19p13.1. In more than two-thirds of NMC cases, a gene fusion between NUT and BRD4 or BRD3 has been documented [2,[5][6][7]; other variant fusions are rare [6]. Recently, we described a variant NMC in which an NSD3-(nuclear receptor binding SET domain 3-) NUT rearrangement was identified in the primary tissue using 5 -rapid amplification of the cDNA end (RACE); the fusion was validated using fluorescence in situ hybridization (FISH) [8].
Little information is available on the cytological features of NMC. Three reports have described the cytological features of 4 common NMC cases [9,10] and another NMC case in which the gene rearrangement was not analyzed [11], and no information on the cytology of variant NMC is presently available. We herein describe, for the first time, the cytological features of a variant NMC of the lung harboring an NSD3-NUT fusion gene.

Clinical Summary
A 36-year-old woman sought medical advice because of a cough accompanied by wheezing with a 2-month duration. An enhanced computed tomography scan performed at the time of hospitalization revealed a mass (75 × 38 × 35 mm in size) in the left lung that extended to the middle mediastinum; metastatic lesions in the liver, breast, bones, and lymph nodes were also detected [8]. A transbronchial biopsy (TBB) of the lung tumor and an endobronchial ultrasoundguided transbronchial needle aspiration (EBUS-TBNA) of a lower mediastinal lymph node with lung tumor involvement were performed.

Materials and Methods
The aspiration material obtained from the lymph node was separated into two parts; its small portion was available for cytological investigation. The specimen was air-dried and Hemacolor-stained (MERCK, Darmstadt, Germany) at bedside according to the manufacturer's instructions and a portion was fixed in 95% ethanol and stained with Papanicolaou as ordinary methods. The larger part of the aspiration material obtained from the lymph node and the biopsy material taken from the lung tumor were immediately immersed in 20% buffered neutral formalin, fixed overnight, and embedded in paraffin. These specimens were then sectioned and used for hematoxylin-eosin staining, immunohistochemistry with antibodies for EMA (DAKO, Glostrup, Denmark), p63 (Santa Cruz Biotechnology, Dallas, TX, USA), cytokeratin AE1/AE3 (DAKO), cytokeratin CAM 5.2 (Becton, Dickinson and Company, CA, USA), CD138 (DAKO), vimentin (DAKO), and others, and FISH, as reported previously [8].

Cytological
Findings. An overview of the Papanicolaoustained smear showed the specimen to be highly cellular with loosely cohesive cells and/or isolated cells ( Figure 1). The cells were 2-2.5 times greater in diameter than that of a small lymphocyte. The nuclei were round to oval in shape and had slightly irregular contours, with one or more prominent nucleoli ( Figure 1). The chromatin was hyperchromatic and finely granular in most of the cells or vesicular in occasional cells. The main cells, which had scant cytoplasm and an indistinct cell border, often formed loosely cohesive clusters ( Figure 2). These clusters were in contact with foci of stratification (Figure 2), which consisted of occasional cells with a clear cell border and moderately delicate cytoplasm, often with cytoplasmic coarse vacuoles (Figure 3, arrowhead) that were negative for epithelial mucin. In addition, a few cells showed an overt pearl formation, including a dyskeratocyte (Figure 3, arrow), whereas occasional small apoptotic cells with orange G-colored cytoplasm were scattered throughout the specimen. A glandular structure was not observed.     On the other hand, the Hemacolor-stained smear showed occasional cells bearing delicate basophilic cytoplasm focally with several cytoplasmic fine vacuoles ( Figure 5), whereas these cytoplasmic fine vacuoles were not identified in the Papanicolaou-stained smear material.

Histological, Immunohistological, and FISH Findings.
Details of the histological and molecular features of this case have been previously reported [8]. Briefly, only a small amount of biopsy was available for histological investigation, and it revealed an undifferentiated neoplasm with necrosis ( Figure 6). In this material, squamous differentiation, which is a possible characteristic of variant NMC, was not apparent.

Treatment and Follow-Up.
The patient received chemoradiation therapy for 10 months after her diagnosis. However, the patient died with disease progression at 10 months after the diagnosis. Autopsy was not permitted.

Discussion
The histological features of NMCs range from entirely undifferentiated carcinomas to carcinomas with prominent squamous differentiation [2,7,[12][13][14][15]. Thus, a diagnosis of NMC based solely on morphology can be difficult. Previous studies have described the cytological features of 4 common NMCs harboring a BRD3/4-NUT fusion gene [9,10] and another NMC case in which the gene rearrangement was not analyzed [11]; the cytological characteristics of these NMCs showed a highly cellular, predominantly noncohesive pattern of relatively small cells with a round nucleus, scant cytoplasm, irregular nuclear contours, variably prominent nucleoli, and identifiable mitotic figures. These findings were also observed in the present variant NMC case. Thus, the previously reported findings imply that the cytological characteristics of NMC are nonspecific and similar to those of undifferentiated carcinoma.
Keratinization has not been identified in previous studies examining the cytology of NMCs [9][10][11]. In theory, overt keratinization is very important and sometimes pathognomonic for a scrutinized diagnosis of NMC; that is, it is assumed to be a relatively characteristic finding of NMC harboring a NUT gene rearrangement involving a gene other than BRD4 [16]. Keratinization in surgical material, however, is notorious for evading observation because its distribution is often very focal [6,16], and sampling biases often occur. The cytology of the present NMC variant showed overt pearl formation, including a dyskeratocyte, and the stratification in contact with loosely cohesive clusters; we believe that this finding corresponds to abrupt keratinization, which has been reported as a peculiar characteristic of variant NMC [16] but was not recognized in our corresponding histology specimen [8]. Thus, the combination of cytological and histological findings may help to reveal keratinization concurrent with stratification in undifferentiated carcinoma, which is one clue for a diagnosis of NMC, especially variant NMC, rather than other tumors.
The Hemacolor-stained smear showed occasional cells possessing delicate basophilic cytoplasm with several fine vacuoles, which were not observed in our Papanicolaoustained smear. In a previous NMC case harboring a BRD3-NUT fusion gene, the cells were described as possessing delicate to finely vacuolated cytoplasm, although we could not observe the cytoplasm in detail because of the low magnification of the published figures [10]. On the other hand, these cytoplasmic fine vacuoles were not observed in NMC cases harboring a BRD4-NUT fusion gene [9]. Further studies are needed to clarify whether these cytoplasmic fine vacuoles are specific for NMC harboring a fusion gene involving NUT and a gene other than BRD4.
In conclusion, although the distinction of NMC from other poorly differentiated carcinomas based solely on morphology is difficult, cytological investigation is helpful, especially for identifying abrupt keratinization, which histopathological investigations can miss because of sampling biases. Our experience has shown that the identification of the following clues may suggest a diagnosis of NMC; overt pearl formation including a dyskeratocyte, stratification, and cytoplasmic fine vacuoles, especially in cases where the initial suspected diagnosis was "undifferentiated or poorly differentiated carcinoma with little pleomorphism. " Furthermore, the identification of this entity is critical, and immunohistochemistry or FISH studies should be considered for the identification of NUT gene rearrangements.