Dietary Pattern and Macronutrients Profile on the Variation of Inflammatory Biomarkers: Scientific Update

It is known that the dietary pattern and macronutrients profile may influence the expression and secretion of inflammatory biomarkers, and the low-grade inflammation is associated with the manifestation of noncommunicable chronic diseases. Therefore, this review aimed to present and discuss the role of dietary patterns and macronutrients on the variation of inflammatory markers related to NCD risk. Scientific evidences within the last five years based on clinical trials, case-controls, cohorts, and cross-sectional studies indicate that normocaloric, carbohydrate-moderated, low-glycemic index, protein-moderated, monounsaturated and polyunsaturated fatty acid-rich, omega-3, and low-saturated fat diets display positive effects on the inflammatory state, both in healthy individuals and in those with cardiovascular risk, although the second group seems to benefit more from changes in the dietary profile.


Introduction
Low-grade inflammation refers to a series of metabolic and physiological modifications with proinflammatory cytokines and increased oxidative stress [1]. It is related to the development and grievance of noncommunicable chronic diseases (NCD), and one of the factors associated with the low-grade inflammation is the intake of foods with proinflammatory characteristics [2,3]. e dietary pattern may be defined as a combination of foods frequently consumed by individuals and population groups [4]. e importance of dietary pattern analysis lies in the fact that the observed effect for a given nutrient or food in environmentally controlled investigations differs from the effect found within the context of different dietary patterns routinely adopted by populations [5].
Indeed, some dietary patterns, such as the Mediterranean and DASH (Dietary Approach to Stop Hypertension) diets, are associated with an improved weight control and lower incidence of NCD [6,7] so that they have been employed as reference or "healthy" diets by the scientific literature and clinical-nutritional practice. Furthermore, the dietary pattern has been investigated as a potential modulator of inflammatory markers [8]. us, the adoption of healthy dietary patterns distinguished by high intake of fruits, vegetables, and low intake of sugar and fats (saturated and trans) seems to decrease the risk of NCD through its influence on the related low-grade inflammation [7,9].
On the other hand, the impact of the distribution of macronutrients-carbohydrates, proteins, and lipids-on the inflammatory state has been extensively investigated [10][11][12][13][14][15], whilst the amount and origin of the nutrients ingested have played an important role in the development of the low-grade inflammation [16]. erefore, this review aimed to present and discuss the role of dietary patterns and macronutrients on the variation of inflammatory markers related to NCD risk.

Materials and Methods
For this review, the electronic databases PubMed (National Library of Medicine, Bethesda, MD) and ScienceDirect were researched. e following keywords were used for article search: "dietary fat"; "fatty acids," "omega-3," "saturated fat," "olive oil," "dietary patterns," "dietary protein," and "dietary carbohydrate" always combined with the MeSH terms "inflammation" or "inflammatory markers." e search was limited to English studies published between March 2012 and April 2017. e inclusion criteria considered original studies published within the last 5 years, in English, which assessed the relation of dietary pattern and macronutrients profile on the variation of inflammatory biomarkers, in observational or interventional study designs. Review papers, editorials and book chapters, case reports, studies with animals or cells, studies that did not aim to evaluate the inflammation, studies that used supplements with registered mark, and studies that assessed the relation between inflammation and diseases other than diabetes, dyslipidemia, metabolic syndrome, obesity, and cardiovascular diseases were excluded from the present review.
Initially, 9429 papers were found. Of these, 9183 papers were excluded after title and abstract screening, and 246 papers were fully read. Of these, 40 papers met the inclusion criteria and were kept for this review (Table 1). In order to complement the discussion, other papers related to the issue addressed were used, despite the year of publication.

Dietary Patterns.
e dietary patterns analysis helps to understand the factors that contribute to NCD [17,18]. erefore, in the last decades, several authors have been dedicated to investigate the association between dietary patterns and indices that evaluate the quality of the diet with inflammatory markers instead of investigating isolated nutrients [3,19,20]. e analysis of dietary pattern can be a priori, when a score is attributed to the diet according to previous knowledge concerning dietary disease health outcomes, or a posteriori, when statistical techniques are used to explore new patterns [21]. Among a priori techniques, several indexes have already been developed, such as the Mediterranean Diet Score [22], Healthy Eating Index [23], Diet Quality Index [24], and Dietary Inflammatory Index [25]. Briefly, these indexes assess the conformity of individuals' diet to guidelines and dietary recommendations by assigning an interpretable score and have been adapted to make improvements and meet the specificities of each population [26][27][28].
On the other hand, the cluster and factor analyses are the most frequently used a posteriori techniques [29,30]. ey allow, respectively, to group individuals with similar food intake or to establish standards from correlating foods. Both approaches, a priori and a posteriori, have limitations and positive aspects, so there is no better technique and they are used to investigate different questions [21].
Regardless of the approach used, it is a consensus that dietary patterns deemed healthy displays as an inverse relationship with the concentrations of inflammatory biomarkers [5,31,32]. Dietary patterns that potentially combat the inflammatory state and reduce the risk for NCD are characterized by the elevated intake of vegetables, green leaves, whole grain cereals, fruits, chestnuts, fish and olive oil, low consumption of embedded meat, sugary drinks, processed foods, and saturated fat [33,34].
In this context, Corley et al. [13] defined two types of dietary patterns: the "Mediterranean diet pattern" and the "conscious consumption pattern," which involves higher fruit consumption and low consumption of meat, eggs, and liqueurs. Inverse associations were observed between fibrinogen and Mediterranean diet. In turn, the individuals who obtained higher scores in the "conscious consumption pattern" displayed lower serum concentrations of C-reactive protein (CRP) (≤3 mg/L). In this study, both patterns were considered healthy, thus reaffirming the importance of diversifying foods for health maintenance. For the elderly, a higher consumption of fruits, vegetables, fowl, fish, and low-fat dairy products is related to lower systemic inflammation [30].
On the other hand, in the study by Bédard et al. [12] with an adult population, the introduction of a dietary pattern in the Mediterranean diet was monitored during a four-week period, with the purpose of evaluating potential modifications in the serum concentrations of CRP. Men who displayed elevated CRP concentrations (≥2 mg/L) showed a reduction in concentrations during the study, while an increase was observed in men who presented normal concentrations (<2 mg/L). In general terms, this study did not report effective results by the Mediterranean diet, which may be related to the reduced follow-up time, diet composition, and control of variables that might have influenced the participants' inflammatory state. Hermsdorff et al. [35] conducted an intervention with adult individuals during eight weeks, in which the adherence to the Mediterranean diet occasioned reduction in the CRP concentrations as well as other inflammatory markers. ese results corroborate other interventional studies [36,37], thus indicating the anti-inflammatory role of the Mediterranean pattern.
In turn, the occidental dietary pattern, characterized by the excessive intake of calories, salt, fat, and sugar, has been associated with the increase of low-grade inflammation and with the development of the NCD [38,39]. Within the adult population, the following patterns were identified: "pastas," "sandwich," "starchy vegetables," "sugary drinks," "dessert," "breads," "fowl," "frozen food," "alcoholic beverage," and "pizza." After the analysis of the inflammatory markers, all patterns displayed elevated CRP (>3 mg/L) at the same time as the highest average was that of the "frozen food" pattern [40].
A similar effect was observed in the study by Kong et al. [5], who evaluated the food intake of overweight individuals, whereas three dietary patterns were identified, according to similarity groups: "high intake of sugar, fat, and salt" (Group 1), "higher intake of water, yogurt, cereals, eggs, and chestnuts" (Group 2), and "higher intake of fruits, yogurts, soups, and lower sugar intake" (Group 3). Differences regarding the inflammatory markers-CRP and interleukin 6 (IL-6)-were not observed between the groups. However, after the evaluation of the concentrations of CD136-a transmembrane protein found in the adipose tissue, with anti-inflammatory function-Group 3 displayed higher concentrations.
Similarly, when considering the foods altogether, we can stablish how anti-or proinflammatory a diet can be [8,41]. In this context, Ozawa et al. [15] conducted a study with adults and gave factorial weights to the foods, thus indicating how pro-or anti-inflammatory they were. From this distribution, participants' diets were assessed, and those who reported dietary patterns considered inflammatory (higher consumption of red meat and processed and fried foods), consequently displayed higher IL-6 levels.
e interconnection between nutrients and health should not be assessed in isolation, once it is known that nutrients from the foods act synergistically in the body and may interact with each other [42]. e foods included in a diet may directly influence the individual's health. Fruits and vegetables are foods that, when consumed on a daily basis in an adequate amount, are capable of reducing the oxidative stress and low-grade inflammation markers [9,43].
Lee et al. [32], after studying an adult population, identified different dietary patterns, named "coffee pattern," "fruit pattern," "meat pattern," and "vegetable pattern." e individuals with a higher score for the "vegetable pattern" displayed a lower CRP concentration, as well as a higher antioxidant intake. A similar result was reported by McGeoghegan et al. [44], who identified two dietary patterns: "1" and "2", these being the ones with higher and lower antioxidant and anti-inflammatory loads, respectively. Pattern "1" was inversely associated with the CRP concentrations and lower prevalence of diabetes.
It is known that fruits and vegetables have various bioactive and antioxidant compounds [45][46][47] and that, when associated with whole grains, fish, and lean meat, may reduce the risk factors with respect to the development of several diseases [48].
is association was verified in the study by Abete et al. [49], who investigated a population of healthy individuals with a history of stroke and identified the patterns named "healthy" and "not healthy." Healthy individuals showed higher adherence to the "healthy" pattern and displayed lower CRP concentrations. In addition, the adherence to the "healthy" pattern was a protective factor for the development of stroke. e studies presented in this review (Table 2) showed the importance of an integral nutrition, particularly to base health promotion actions. e adherence to patterns deemed healthy, such as the Mediterranean diet, and patterns that included fruits and vegetables and reduced intake of processed food has been associated with the reduction of inflammatory and systemic markers, as well as with the risk of NCD [50]. erefore, it is possible to verify that a balanced and varied nutrition, rich in natural foods and with the least amount of processed foods, is capable of reducing the lowgrade inflammation and associated diseases. In addition, it was possible to observe that the dietary patterns identified display similar characteristics and effects, regardless of the population, thus highlighting the importance of assessing the cumulative associations of certain groups of foods.

Lipids.
e proportion and type of fats included in the diet influence the degree of inflammation and the risk of NCD. In this context, the intake of polyunsaturated fatty acids (PUFA), particularly the long-chain ones from the w-3 series (n-3 PUFA), is related to the reduction of the risk of cardiovascular diseases and death due to its antiinflammatory potential [51][52][53][54]. Diets high in monounsaturated fatty acids (MUFA) have also been highlighted for their potential in reducing inflammation due to the lower expression of genes related to the synthesis of IL-6 [10,55].
Saturated fatty acids (SFA), on the other hand, favor the increase of inflammation. Most studies included in this review related the consumption of SFA to the increase of inflammatory markers and NCD. Kantor et al. [56], in a retrospective cohort with elderly, observed that the lower ingestion of SFA was associated with the reduction of CRP in eutrophic and overweight groups, but not in obese, thus suggesting that the modulating role of the SFAs in the CRP concentrations is mediated by the individual's nutritional state. Lesná et al. [57] found reduction of CRP and IL-8 in a three-week randomized crossover study, after replacement of an SFA-rich diet (42% of the total caloric value "TCV") by a PUFA-rich diet (40% of the TCV). e increase in the expression of proinflammatory cytokines associated with the consumption of SFA is due to the activation of toll-like receptors (TLR), in particular, the TLR-4. erefore, transcription factors such as the factor nuclear kappa B (NF-κβ) are stimulated, resulting in the synthesis of proinflammatory cytokines [8,58]. Silver et al. [59] investigated the effect of a hyperlipidemic diet (50% of the TCV), with equal proportions of MUFA, PUFA, and SFA, on overweight individuals, and observed, after two weeks, an increase of 6% in fat oxidation, an average reduction of 2.5 kg of body fat, an average increase of 2.5 kg of lean mass, and a reduction in the interleukins IL-1α, IL-1β, 1L-12, and IL-17, besides interferon gamma (IFN-c), tumor necrosis factor-alpha (TNF-α), and tumor necrosis factor-beta (TNF-β) concentrations and improvement in vascular function. In this context, hypolipidemic diets may not be the best strategy to reduce NCD, as the fatty acids profile (MUFA, PUFA, and SFA) is more relevant for modulating the inflammation than the proportion of total lipids in the diet. In addition, diets with higher lipid content are more palatable and provide greater satiety and better weight control [60].
With respect to the linolenic acid, an intervention with type-2 diabetics resulted in reduction in the IL-2 concentrations and TNF-α after n-3 supplementation (eicosapentaenoic "EPA" 1.548 mg/d; docosahexaenoic "DHA" 828 mg/d; and others n-3 338 mg/d) during eight weeks, although CRP levels were maintained [61]. Rajkumar et al. [62] reported an improvement in the proinflammatory profile with CRP reduction in overweight patients after n-3 supplementation (EPA 180 mg/d and DHA 120 mg/d) during six weeks. Ito et al. [63] observed the same effect with dyslipidemic individuals. In turn, Itariu et al. [64] verified a reduction in the gene expression of inflammatory biomarkers in the subcutaneous adipose tissue, a reduction in IL-6 concentration, and increased anti-inflammatory eicosanoids in the visceral adipose tissue, after n-3 supplementation (EPA 460 mg/d and DHA 380 mg/d) during eight weeks. In another study, the authors observed a CRP reduction after eight weeks of n-3 supplementation (EPA 720 mg and DHA 480 mg) in men with coronary artery disease [65].
Accordingly, the beneficial mechanism of the linoleic acid (n-3 series) has been related to its derivatives, the EPA C20 : 5 n-3 and DHA 22 : 6 n-3 acids, which have a higher anti-inflammatory effect than the derivatives of the arachidonic acid (AA n-6 PUFA) derived from the linoleic acid (n-6 series). Such a effect was reported in a study with adults, in which an increase was reported in the formation of EPA and DHA after four months of supplementation (1.4 g/d) in comparison with the placebo group (soy oil), although with no changes in AA metabolites [66]. Hence, the cause of reduction in inflammation was the synthesis of antiinflammatory metabolites, rather than the suppression of proinflammatory metabolites derived from the AA.
However, investigations about the effect of n-3 lipids on NCD-related biomarkers reported contradictory findings. e cardiometabolic risk of the sample population seems to influence the results, as the beneficial effects are more evident in individuals with more than one risk factor, rather than excessive body fat alone [67,68]. Studies in which the population had metabolic syndrome (MS), diabetes mellitus (DM), and/or dyslipidemia [32,61,63] reported more pronounced results in the improvement of proinflammatory biomarkers than studies with individuals who were overweight only, with no other associated diseases/risk factors [67][68][69]. Indeed, some studies did not find evidences that justify the n-3 supplementation [67,68,[70][71][72]. Dewell et al. [70] assessed the effect of a low-(2.2 g/d) and high-dose (6.6 g/d) plant-derived n-3 supplementation, compared to the low-(1.2 g/d) and high-dose (3.6 g/d) sea-derived n-3 supplementation but did not find differences between the groups for IL-6 and soluble intercellular adhesion molecule-1 (sICAM-1), after eight weeks. Nigam et al. [71] assessed the effect of n-3 supplementation (4 g/d) during 271 ± 129 days on average and reported that, after 6 months, the reduction of CRP and myeloperoxidase (MPO) was similar to that observed in the placebo group. Darghosian et al. [72] also observed the maintenance of IL-6, IL-8, IL-10, TNF-α, and monocyte chemoattractant protein after 6 months of n-3 supplementation (4 g/d). Different proportions of n-3 in the diet did not affect the IL-6, monocyte chemoattractant protein-1 (MCP-1), concentrations TNF-α 1 and 2 receptors, and PCR, after 14 weeks of n-3-rich diet (3.5% of TCV), compared to the group with a diet poor in n-3 (0.5% of TCV) [67]. e differences observed in the inflammatory profile may be related to variations in the intervention time and in the supplemented daily dose. e daily doses used varied between 1 and 6.6 g/d, whereas doses above 3 g/d were less common. Another aspect worth considering is the origin of fatty acids of the n-3 series, which may be plant or seaderived. Only one study included in this review, conducted by Dewell et al. [70] assessed the effect of different n-3 sources, although a beneficial effect was not found in any of the inflammatory markers evaluated, regardless of the dose or source. However, since most of the studies did not find evidences of health damage caused by n-3 supplementation and most of them reported benefits from its use, the usual intake of foods that are source of n-3 may be a strategy to reduce risk or grievance of diseases associated with the inflammation, particularly in patients with chronic diseases, whereas the use of supplementation demands further investigation.
Furthermore, the relation between linoleic (CLA) and linolenic (LNA) acid intake is very important for homeostasis [73]. After being ingested and absorbed, these fatty acids are metabolized and underwent elongation, desaturation, and retroconversion reactions. e action of cyclooxygenase and lipooxygenase enzymes on these fatty acids leads to the formation of different eicosanoids (prostaglandins, leukotrienes, thromboxanes, etc.), whereas the derivatives of the n-3 series are more anti-inflammatory and those of the n-6 series are more proinflammatory. e Cardiology Research and Practice importance of balance for the n-3 : n-6 ratio is due to the fact that the desaturase δ-6 enzyme has greater affinity with LNA than with CLA. erefore, in order to avoid an imbalance in the production of anti-and proinflammatory eicosanoids, studies suggest that the CLA ratio in the diet is greater than the LNA ratio, whereas the appropriate ratio is around 4 to 5 : 1 and not superior to 10 : 1 [74]. In its turn, the extravirgin olive oil, mainly composed by MUFA (w-9 series)-oleic acid-has reduced the risk of NCD when used as supplement or consumed within the context of healthy dietary patterns [75]. Martínez-González et al. [76] reported a reduction in the risk of atrial fibrillation in individuals with high risk of cardiovascular disease, after intervention with Mediterranean diet supplemented with extravirgin olive oil. In another study, individuals with cardiovascular risk submitted to the Mediterranean diet supplemented with 50 ml/day of extravirgin olive oil reduced the CRP, IL-6, sICAM, and P-selectin concentrations, when compared to the control group, which was provided with a hypolipidemic diet [7]. Ceriello et al. [77] also compared the effect of hypolipidemic and Mediterranean diets, although without oil supplementation, and reported that, after three months, there was a reduction of IL-6, intercellular adhesion molecule 1 (ICAM-1), and 8-isoprostaglandin F2α (8-iso-PGF2α) only in the group to whom the Mediterranean diet was offered.
Nevertheless, the concentration of phenolic compounds in the oil, capable of reducing the synthesis of AA and cyclooxygenase enzymes 1 and 2 (COX-1 and COX-2), may be a key factor in its potential to reduce inflammatory markers. Camargo et al. [78] compared the effect of three meals prepared with virgin oil classified according to the content of phenolic compounds as high (398 ppm), intermediate (149 ppm), and low (70 ppm). e participants who were provided with meals with high content of phenolic compounds displayed greater reduction in the IL-6, IL-1, and CXC motif chemokine ligand (CXCL) expression than the other groups. Indeed, oil consumption is associated with a lower inflammation degree, particularly in the context of the Mediterranean diet, and its use indicates an important nutritional strategy for the reduction of cardiovascular diseases [79]. e large number of studies devoted to investigate the relation between lipids and inflammation highlights the importance of this macronutrient to the modulation of several inflammatory biomarkers (Table 3).
us, the literature indicates that adjusting the proportion of SFA, MUFA, and PUFA in the diet is a more effective strategy to modulate the inflammation than the use of hypolipidemic diets, which may play the inverse role. In addition, the higher SFA consumption is related to the increase of inflammatory biomarkers, and this increase is proportional to the ingested amount. us, choosing foods rich in MUFA and PUFA and avoiding foods with high SFA content may be beneficial, with respect to the modulation of the inflammation and reduction of the risk of diseases associated with it. Among the alternatives, it is possible to highlight the consumption of extravirgin olive oil with high phenolic compounds content, which supports not only the reduction of inflammation but also the improvement of the endothelial function. Despite the high number of studies available, there is still limited information about the influence of the nutritional state in the modulation of lipids on the inflammation, since obese individuals seem to be less responsive to the benefits of an adequate lipidic distribution.

Proteins, Carbohydrates, and Glycemic Index.
Proteins are the macronutrients of greatest thermogenic effect due to their high cost of metabolic synthesis, hydrolysis, gluconeogenesis, and excretion as urea [10]. In addition, the protein content of the diet may lead to increased satiety and decrease in calorie intake [80,81]. e effects on human health arising from the ingestion of several protein types have been investigated, although their association with metabolic diseases is still conflictive [80]. A study with a Mediterranean population, particularly from Greece, who consumed diets based on plants, vegetables, fruits, and cereals, shortly after World War II, reported lower mortality rates from cardiovascular diseases, due to the low amounts of protein in relation to heme-iron [82]. Nevertheless, in the context of a western diet, a high iron bioavailability was reported, mainly because of the ingestion of heme-iron-related animal protein, which favors an antiinflammatory state [83].
Currently, iron has been reported as a promoter of atherosclerosis due to its ability to produce free radicals [84]. According to Vallianou et al. [85], in their study with 490 middle-aged Caucasian adults who were apparently healthy-in which they evaluated the protein ingestion of the diet, taking into account the animal and vegetable protein-the serum concentration of cystatin C decreased as the frequency of animal protein increased and the platelet count decreased with the increase of vegetable protein intake, whereas no association was found with monocytes, lymphocytes, and CRP. e influence of protein in the diet on postprandial inflammation is still uncertain. Arya et al. [86] reported that, in healthy individuals, meat with high fat content is more proinflammatory, in comparison with lean meat, in relation to TNF-α and IL-6.
Azadbakht and Esmaillzadeh [87] verified that the consumption of red meat was related to higher plasma concentrations of CRP in women. Cocate et al. [88], in a study with 296 men between 40 and 59 years of age, observed a higher occurrence of central obesity, hypertriglyceridemia, and metabolic syndrome in individuals within the first tertile of BCAA (branched-chain amino acids) and leukin consumption, in comparison with those from the second and third tertiles; besides, the higher BCAA and leukin consumption has been considered a protective factor for central obesity and metabolic syndrome. In addition, in another study with the same sample, Cocate et al. [89] verified that the increased consumption of red meat was associated with a higher occurrence of metabolic syndrome and hypertriglyceridemia. Furthermore, a cross-sectional study with 553 individuals aged between 18 and 80 years verified that the consumption of processed meat showed positive association with IL-6, TNF-α, TNF-R1, and   Compared to DHL + P, DHL + S had higher effect on the IFN-γ reduction (↓74%) and DHL + L had higher effect on PAI-1 reduction (↓ 31%) Hence, they were randomized into the following groups for 14 weeks: TNF-R2, even after adjusting for consumption of fruits and vegetables. In turn, the consumption of unprocessed red meat was inversely associated with TNF-R1 and TNF-R2 [90]. us, the higher protein and amino acid intake from red meat is directly related to cardiometabolic diseases, whereas the variation of inflammatory markers also seems to be involved. Dairy products are also a food group deemed as an important protein source. Hence, Zemel et al. [91] found an inverse relationship between the consumption of dairy products and inflammation, both in overweight and obese individuals. In a randomized case-control study, which assessed the relationship between different protein sources, the authors observed that, after intervention, there were lower CC5-chemokine binding to the chemotactic monocyte-1 protein, which facilitates adherence and transmigration of monocytes through the arterial wall, thus leading to an inflammatory process-concentrations in individuals who consumed whey, in comparison with those who consumed cod and casein, in addition to lower CC5 concentrations in individuals who consumed gluten, in comparison to those who consumed cod [92]. In turn, MCP-1 displayed higher values in individuals who consumed whey, in comparison to those who consumed cod and gluten, which can be explained by specific milk properties, such as increased insulin response, as whey is more insulinotropic than cod, gluten, and casein protein. As a general result, whey showed better anti-inflammatory effects, which suggest that this protein source is relevant for the modulation of inflammatory biomarkers.
Amini et al. [93], in a study with 56 Iranian women, engaged in physical activities, found a marginally significant decrease in the CRP concentrations after intervention, both in the hyperproteic (HP) and the balanced (B) diets. is finding, which, unlike the others, did not find any advantage of the HP diet, may be justified by the effect of physical activity, and not only the diets, as a protective factor.
In addition to the studies that assessed the amount and quality of protein associated with the NCD and chronic subclinical inflammation, the quality and amount of carbohydrates or the glycemic index have been highlighted within the scientific literature (Table 4). Cross-sectional studies have demonstrated an inverse association between the intake of whole-grain carbohydrates and low-grade inflammation [94]. However, recent studies about the replacement of refined whole-grain wheat products were inconclusive [95]. erefore, in general, the consumption of whole-grain products is inversely related to inflammation.
For example, Montonen et al. [96], in a study with 2198 individuals from the European Prospective Investigation into Cancer and Nutrition (EPIC), observed an inverse correlation between the consumption of whole bread and CRP and gamma-glutamyl transferase (GGT) values, as well as a direct correlation between the consumption of red meat and the same inflammatory markers.
With respect to simple carbohydrates, its elevated consumption is worrying, especially when added in foods and drinks, thus favoring the inflammatory state and negative health effects. e World Health Organization (WHO) recommends that children and adults reduce the sugar intake to <10% of total energy, and that this value should be lowered over lifetime [35].
In this context, a study with eutrophic individuals, aged between 20 and 80 years, which aimed to determine the effect of the chronic fructose (55%) consumption, sucrose, and honey on glucose-tolerant (GT), as well as glucoseintolerant (GI) individuals, reported that the GI group displayed serum CRP concentrations > 3 mg/L before and after intervention, and these values were lower to those observed in the acute inflammation, which may indicate an increased risk of cardiometabolic disease [100]. CRP concentrations of the GT group were normal (<3.0 mg/L), although there was a nonsignificant increase after the intervention. IL-6 values were also greater in the GI group. Aeberli et al. [101] observed a CRP elevation after isolated fructose, glucose, or sucrose consumption, while it is important to highlight that the sucrose dose was higher than that of the previous study (80 versus 50 g/d), as was the intervention period (2 versus 3 weeks).
In turn, with respect to the studies with adults, which associate the ingestion of protein and carbohydrates/glycemic index to the ingestion of hyperproteic (10-15% of TCV), HP (23-28% of TCV) [106], low-glycemic index (LGI), highglycemic index (HGI) [97], HP + HGI, HP + LGI, LP + HGI, and LP + LGI, in relation to the inflammation, the authors observed that HP diets increased, while B diets decreased, the CRP levels, respectively.
Studies that assessed the effects of GI and protein intake on the inflammatory markers in children are scarce. e study Diet, Obesity, and Genes (Diogenes), found an increase in body fat in children who consumed a HP + HGI diet, whilst the percentage of overweight children decreased in the group who consumed the HP + LGI test diet [107]. In this same study, a decrease was observed in children and adolescents' CRP between 5 and 18 years of age who consumed a LGI diet [106]. is is in contrast with the study by Diogenes with adults, in which CRP concentrations decreased in individuals who consumed hyperproteic diet with HGI [104].
is may be explained by the lower diet adherence by the families and by the fact that the decrease of GI reached less than 4 difference points after the nutritionists' assistance to the families within the study [98].
us, it is possible to acknowledge that the lower consumption of protein from red meat and the higher consumption of proteins from vegetables, white meat, and milk displayed beneficial health effects and may lead to the decrease of inflammatory markers. However, there is still lack of consistent information about the effect of protein origin and amount on the inflammation, particularly in healthy people. With respect to GI and carbohydrates, results indicate that the moderate ingestion of carbohydrate, as well as of food  (ii) e red meat group was composed of lean meat, high-fat meat, ground beef, lean pork meat, high-fat pork meat, and bacon (iii) e white meat group was composed of chicken with skin, skinless chicken, and fish (iv) Blood samples after 12 h of fasting: assess ox-LDL Each smoothie shake contained 170 kcal, 10 g of proteins, 1 g of fat, and 30 g of carbohydrates CRP exhibited an global treatment effect, which resulted in a significant reduction (57%) through the ingestion of skimmed milk smoothie (iv) Blood samples: assess inflammatory markers with LGI, may have a beneficial effect on the metabolic and inflammatory states in obese individuals, while in children and adolescents, the LGI displayed an inverse effect.

Conclusions
From this literature review, we reiterated the importance of a varied diet, rich in natural foods and poor in processed foods, such as some previously known patterns (Mediterranean and DASH diets) to prevent and improve low-grade inflammation. With respect to the macronutrients, the provided consumption of PUFAs and MUFAs, as replacements for SFA (moderate intake of carbohydrates, in particular the ones with low glycemic index, and the consumption of proteins, in particular the ones of plant origin and white meats), may positively influence the modulation of low-grade inflammation and, consequently, has a protective effect for the development of the NCD.
However, further studies are necessary, in order to evaluate the inflammatory response to the consumption of dietary patterns or intake of specific macronutrients in different situations of weight, age, and metabolic condition, for a better understanding of the most appropriate doseresponse.

Conflicts of Interest
Helen Hermana Miranda Hermsdorff and Sylvia do Carmo Castro Franceschini are fellows of National Council for Scientific and Technological Development (CNPq) Research Productivity. e funding received did not lead to any conflicts of interest. Also, there are no other conflicts of interest regarding the manuscript. Female volunteers were grouped equally and randomly in two diet groups: ere was a marginally significant decrease in CRP levels after intervention, both in the HP and LP diets (i) HP diet: 45% carbohydrates, 25% protein, and 30% fat (ii) LP diet: 55% carbohydrates, 15% protein, and 30% fat (iii) All of them displayed a reduction of 500 kcal from the conventional diet Dietary intake was determined by a food record applied on baseline and every 2 weeks, referring to 3 days of the week (iv) HP and LP diets were provided in an 8-week period (v) Blood samples: assess CRP as inflammatory parameter