The Relationship between Plants Used to Sustain Finches (Fringillidae) and Uses for Human Medicine in Southeast Spain

We analyzed plants that are traditionally used by wild bird hunters and breeders to capture and promote captive breeding of Fringillidae (finches or songbirds) in the province of Alicante, Spain. The majority of plants used in songbird breeding have medicinal properties in traditional human medicine (48 different uses); thus, another main goal was to show their relationships with human medical uses. We compiled a list of 97 plant species from 31 botanical families that are used to attract finches and identified 11 different use categories for these plants in finch keeping. The most common uses were for trapping birds and as a source of food for birds in captivity. Cannabis sativa has the greatest cultural importance index (CI = 1.158), and Phalaris canariensis (annual canary grass or alpist) was the most common species used to attract Fringillidae and was used by all informants (n = 158). Most of the 97 species are wild plants and mainly belong to the families Compositae, Gramineae, Cruciferae, and Rosaceae and also have medicinal properties for humans. In the study area, the intensification of agriculture and abandonment of traditional management practices have caused the population of many songbirds to decline, as well as the loss of popular ethnographic knowledge.


Introduction
Throughout the ages, the human race has used plants for various purposes [1], particularly those that are accessible. In the Iberian Peninsula, several studies have been developed on medicinal plants [2][3][4][5][6][7][8] and edible flora [9,10], as well as some general ethnobotanical studies [11][12][13][14][15], and others about the importance of home gardens and cultivated areas in the evolution of useful flora [16]. However, few studies have described the use of plants in ethnoveterinary medicine [17,18], or in attracting and maintaining birds of the Fringillidae family in captivity [19,20]. Plants have been used in traditional medicine for several thousands of years to treat and cure diseases in domestic animals and human populations, especially native ones [21,22]. Furthermore, in nature, wild birds use particular plant species, which possess insecticidal and bactericidal properties, to build their nests.
This practice creates optimal conditions for egg laying and incubation [23].
The ecological knowledge of local traditional uses that depend on the dynamics of natural resources has been reflected in numerous studies [24][25][26], considering the ecological knowledge of local communities of hunters, anglers, and gatherers [27].
The culture of capturing songbirds was introduced to the Iberian Peninsula by the Romans and had its beginnings, as did other forms of hunting, in the absolute necessity of human nutrition. Thus, these birds were traditionally caught as a source of food in Valencia, at least since the 17th century [28]. Today, following old customs and culinary habits, there are still hunters who hunt this group of birds in order to eat them. On the other hand, the term "pajareros" describes people who are dedicated to hunting, breeding, or selling birds [29]. Although these birds are not hunted excessively, it is essential to monitor and control illegal methods of hunting Fringillidae and to conserve this family of birds [30].
The capture of birds using a hinged net assembly is a traditional hunting method that is widespread in the province of Alicante and elsewhere in the Iberian Peninsula. These game nets are made with cotton, hemp, or nylon mesh. They are placed on the ground and have a manual activation system; once a bird enters the net, a rope is pulled to trap the bird inside the net (see Photographic annex). The nets used since the middle ages to capture several species of Fringillidae intended for use as pets are well known among the inhabitants of this zone [31,32]. These birds are relatively easy to maintain and rear in captivity, and it is easy to train them to participate in singing competitions. Thus, at present, the capture of five species of birds (Serinus serinus, "verdecillo", Carduelis carduelis, "jilguero", Carduelis chloris, "verderón", Carduelis cannabina, "pardillo," and Fringilla coelebs, "pinzón") is authorized and regulated by law (Council Directive 79/409/EEC and national Laws 4/1989Laws 4/ , 62/2006Laws 4/ , and 13/2004. What is more, it is an important cultural movement around the Mediterranean Basin [33]. The current trend is to increase breeding in captivity and reduce the capture of wild birds. Therefore, it is important to acquire more knowledge about the traditional use of cultivated and wild plants. The main aim of this paper is to document the crosscultural comparison between plant uses for songbirds and humans in Mediterranean environments, relating an ethnoveterinary field study and its eventual link to folk therapies for humans, in order to preserve ethnological knowledge on European folk health. With this purpose in mind, the information on plant uses for songbirds (capturing, feeding, and breeding) gathered here was collected during fieldwork and complemented with ethnobotanical references. Finally, we would like to contribute to the dissemination of results within the scientific community in order to open a door to research in other disciplines.

Study Area.
The province of Alicante is located in the southeast region of Spain, in the southern part of Valencia. It is geographically located between the coordinates 38 • 30 N and 0 • 50 E (Figure 1). The total area occupied by the province is 5,863 km 2 , it has a population of 1,783,555 inhabitants, and there are 141 localities. The province has a very mountainous and rugged relief, except for some river valleys. Thus, approximately 60% of the study area is located between elevations of 200 and 1,500 m above sea level.
Due to its geographical location, the province of Alicante has a typical Mediterranean climate with mild temperatures. Thus, the average temperatures are between 6.2 • and 16.8 • in the coldest month (January) and 20.4 • and 30.6 • in the hottest (August), with an annual mean of 17.8 • . The average annual rainfall is 336 mm, concentrated in spring and autumn, and there is a prominent dry period in summer. However, there are some climatic differences between the coast and the interior of the province, due to its topography [34,35]. The plant species in the province of Alicante include sclerophyllous shrubs and trees, which are adapted to Mediterranean stress conditions. Local flora, consisting of evergreen, coriaceous, glabrous, and aromatic plants, is adapted to conserve water for much of the year. Some qualities are common to many of these plants, including resistance to drought, adaptations to heat, and low tolerance to low temperatures. These bioclimatic and biogeographical conditions favour the development of rare, endemic, and endangered species [35,36]. Considering its bioclimatic and biogeographical conditions, the province of Alicante may potentially give rise to vegetation that can be divided into three main types: evergreen oak forest (Rubio longifolia-Quercetum rotundifoliae), ash-maple forest (Fraxino orni-Aceretum granatensis), and spiny maquis (Chamaerops humilis-Rhamnus lycioides) [37].

Ethnology.
A total of 69 localities were prospected with oral interviews in all regions of the Alicante province (El Comptat, L'Alcoia, Alt Vinalopo, Vinalopo Mitja, Marina Baixa, Marina Alta, L'Alacanti, Baix Vinalopo, and Baix Segura-Vega Baixa) ( Figure 1). Vernacular names of plant species traditionally used were obtained in the field by interviews with the local population. Ethnological information was based primarily on semistructured interviews, in which we gathered information. This ranged from the different plant species used to attract and maintain songbirds, the season of plant collection, traditional uses of the plant species collected, the composition of commercial mixtures used to feed captive birds, and folk remedies used to cure songbird illnesses, to the environmental problems faced by the community.
People with a specific profile were selected in order to obtain high-quality and reliable information. People interviewed were older (50-85 years old), living in a rural environment and from a variety of socioeconomical strata, who had captured and bred birds throughout their lives. We wanted to emphasize the ethnobotanical importance of local variations of plant names and the different applications of these species. We conducted 158 oral interviews; 95.57% (n = 151) of the informants were male and 4.43% (n = 7) female, and the mean age was 56.7 years. In 48 municipalities, inhabitants speak Valencian (variant of Catalan), and Castilian (standard Spanish) is spoken in the others.
Numerous folk botanical references were examined [38][39][40][41], including a variety of local books [35], magazines [7], and festivals, to obtain information on remedies for animal illnesses. Even though the information included in our analysis arose from an array of different spoken and written sources in the study area, the semistructured interviews revealed many important issues previously unidentified [20].
A digital sound recorder was used to record interviews and to create an audio record of the information. In addition, a photographical archive, with photographs of each of the species referred to by the informants, was constructed and deposited in the Ecology Department Archive of Alicante University.
Evidence-Based Complementary and Alternative Medicine 3 M e d it e r r a n e a n S e a N M e d it e r r a n e a a n n n S e a The information gathered in interviews was further verified by field observations with the stakeholders. This kind of investigation, in sociological terms, is called "participant observation" [42]. In this process, hunters were observed while they prepared for the hunt and during hunting, and their recreational activities were documented. In these field samplings, we also identified species of plants currently used by bird breeders and the techniques used to catch birds.
Plants were collected from various parts of the study area and were identified in the laboratory, using dichotomous keys [43] and registered at the ABH (Herbarium of Alicante University). We used Excel 2003 to perform a simple statistical analysis of the data collected; specifically, we calculated the relative frequency of citation (RFC) [8] at which each species of plant was used to attract birds during hunting and to maintain birds in captivity (in Table 1). Moreover, we calculated a cultural importance index (CI) where each addend is a measure of the relative importance of each plant use [8].
Finally, we related the use of these plants for wild finches with their potential human medical use, by using some important sources of reference for ethnobotanical and alternative medicine for Mediterranean environments in the southeastern part of Spain [7,35,[39][40][41]44]. Plant uses have been grouped according to cures for different ailments [18].

Results
We collected 97 species of plants and another variety of one of these species, belonging to 31 botanical families, which are used for different purposes. We present the scientific names of these plant species, voucher register, the family to which they belong, their main uses in finches, relative frequency of citation, cultural importance, whether wild or cultivated types were used, and their medical properties for humans (Table 1).
Compositae, Gramineae, Cruciferae, and Rosaceae are the families most represented among the plants used to catch and promote breeding of songbirds. In this study, all the species of birds showed a preference for wild species of plants. Evidence-Based Complementary and Alternative Medicine 5    Evidence-Based Complementary and Alternative Medicine 7 Most of the plant species (24.75%) identified were placed inside nets to attract and capture wild birds in the field ( Figure 2). Thus, once birds have entered the nets, the hunter pulls a rope, and the birds are trapped (Figures 3  and 4). The stems of some plants (e.g., Lygeum spartum, Olea europaea and Stipa tenacissima) are spread with an adhesive substance called birdlime ("envisque" or "liga" in local Spanish), obtained from a mixture of resins (e.g., resin from Pinus halepensis and Pinus pinea), olive oil (from Olea europaea), and some plants (e.g., Andryala ragusina, Chondrilla juncea, and Euphorbia characias). Birds that land on these stems while frequenting feeders or watering points are captured in this way. Catching tools include plants that are used to construct hunter refuges (e.g., Arundo donax, Phragmites australis, and Viscum album) or decoys that are used to attract other birds to the nets (e.g., Brachypodium retusum and Hyparrhenia hirta). Capture nets must blend in with the terrain conditions; therefore, they are dyed a matte colour that is as close as possible to the surrounding environment. Hunters use an infusion of certain plants (e.g., Punica granatum, Pinus halepensis, and Pinus pinea) to produce these dyes.
Furthermore, many of the species were used to produce the seeds and wild vegetables (18.81%) used to feed birds in captivity. Plants that facilitate breeding include the ones that are used by birds in captivity to build nests (e.g., Agave americana, Cannabis sativa, Chamaerops humilis, Paronychia argentea, and Phoenix dactylifera), feed their offspring (e.g., Brassica oleracea var. italica), and stimulate mating (e.g., Urtica dioica and Urtica urens). Breeders used the fruits and roots of some plants (e.g., Daucus carota, Fragaria vesca, and Rubus ulmifolius) to enhance the natural red factor in some species of birds, providing natural pigments, particularly in Carduelis cannabina and Carduelis carduelis. Currently, the cages are made principally from metal or synthetic materials; however, informants can identify the specific natural materials that are used to be used to build cages and cage accessories (e.g., Arundo donax, Daphne gnidium, Phragmites australis, and Nerium oleander).
Birds in captivity may suffer from certain diseases, and breeders often try to cure these birds by using natural, plantbased remedies. Thus, there are some vulnerary plants (e.g., Chelidonium majus and Rosa agrestis) and others that stop haemorrhages (e.g., ash of Nicotiana tabacum). Some species have antibacterial properties (e.g., Cicer arietinum, vinegar of Malus domestica, and Citrus limon), or they promote moulting (e.g., Lavandula latifolia), have disinfectant functions to eliminate microbes (e.g., Pistacia lentiscus), or can host beneficial probiotic bacteria or tonic (e.g., vinegar of Malus domestica). Some plants have been used as vermifuge, placed in the breeding carrier, in order to expel parasites (e.g., worms) from the intestines, such as mites (especially Syringophilus sp., Dermoglyphus sp., and Dermanyssus sp.) and lice (Menacanthus sp. and Goniocotes sp.) that affect this group of birds. Leafy vegetables are used as a laxative treatment, the juice of Urtica urens to prevent anaemia, and Cicer arietinum is used to stop diarrhoea. To sum up, we show the number of species that are used with specific bird veterinarian uses in Table 2.
We only found three vulnerary plants for finches; however, there are 13 species of the total used for this use in humans. There are three antibacterial plants in birds, while in humans we found two different species (Portulaca oleracea and Centaurea aspera). One plant is disinfectant for finches, while in humans there are 5 antiseptics to eliminate microbes (Foeniculum vulgare, Centaurea aspera, Pinus halepensis, Lavandula latifolia, and Rubus ulmifolius) and fungal species (Centaurea aspera). Twenty eight species are used as a laxative treatment in birds, whereas only eleven have the same medical use for humans. Conversely, we found no plants that are probiotic or that stop bleeding in humans.

Discussion
Traditionally, nutritive uses [45] and curative applications [46]   wild and cultivated species [47,48], especially in rural areas [7]. In contrast, wild bird hunting is traditionally a maledominated pastime. Therefore, we want to highlight that the stakeholders have high know-how, which reflects their identification of different species and their applications. The names and traditional uses can vary depending on geographical location, as vernacular names serve as intangible heritage. Thus, it is necessary to preserve this heritage and promote educational and awareness programmes [20].
The cultural importance index corresponds with an interest in detailing the specific uses of plants that better reflect the cultural aspects of plant utilization. In fact, ethnobotanical publications usually present plant uses in tables or catalogues, where the information is grouped by species, indicating their particular uses and, commonly, the number of informants who mentioned them. This way of grouping is much more reasonable for evaluating the importance of each plant species by its cultural consensus [8]. This additive index takes into account not only the spread of use (number of informants) for each species, but also its versatility, that is, the diversity of its uses [17]. Thus, Cannabis sativa and Phalaris canariensis have the greatest CI, being the principal commercial seed and, moreover, Cannabis sativa has other uses. In contrast, the lowest CI are in plants that are used to cure or have no typical uses and are not used by informants to breed songbirds.
Various mixes of dried seeds, composed of seeds from different species, both wild and cultivated, are used to feed birds in captivity [49]. Each bird breeder uses the mixture of seeds that he/she deems most appropriate. However, some breeders use leafy vegetables to feed birds and supplement their diet of dried seeds. These plants are used mainly in summer, during the birds' moulting period and as a laxative. Other species not cited in this study, such as Ilex aquifolium, Viscum cruciatum, or Onopordum nervosum [11][12][13][50][51][52], are used to capture birds in other areas. Moreover, some plants also have different veterinary uses in other Mediterranean regions. Thus, some authors show that several species, such as Stellaria media, Avena sativa, and Urtica dioica, are used to increase fertility and egg production in chickens. Urtica urens is mixed with feed for hens so that they lay eggs earlier in their lifespan and as a result, the eggshells will be harder. Cirsium arvense, Daphne gnidium, Phragmites australis, and Linum usitatissimum are antidiarrhoeal and have been used to favour digestion. Allium sativum, Daphne gnidium, Nerium oleander, and Nicotiana tabacum are useful against parasites on farms, and Cicer arietinum is used to facilitate the expulsion of the placenta and for purgation in goats and sheep. Olea europaea is used to treat mastitis or to detoxicate, and latex from Chelidonium majus and Pinus halepensis is used to treat wounds [17,19,21,53]. With these data, we can verify that there is a popular tradition for the use of ethnoveterinary plants in Mediterranean areas.
On the other hand, bird populations have declined, mainly due to the abandonment of crops, the use of pesticides, predation of nests, poaching, increased predation due to changes in their natural habitat, uncontrolled development, and in general socioeconomic changes in recent decades [59]. In this aspect, the mechanization of agricultural practices has changed the structure of these agrarian ecosystems, accompanied by a steady degradation and loss of landscape elements with important ecological functions [60]. To preserve bird populations, it is essential to maintain fields active. There are many plants linked to these environments that birds use daily, such as for food or other purposes.

Conclusions
In conclusion, data obtained in this research are scarcely known and show many details of plants related to songbirds, facilitating access to interesting and novel information. This allows recovery of forgotten uses and traditions, highlighting the utilization of different species to attract and cure birds and their relation to human medicine, and resulting in a very interesting contribution to ethnobotanical bibliography.
We found that the majority of the plant species related to songbirds were wild, reflecting that the wild bird hunters are aware of this preference and exploit this knowledge of wild flora in their hunting. This demonstrates that informants have great knowledge of the plants used in traditional medicine and finch keeping. Also, the majority of species have medicinal properties that can be used for informants to cure different pathologies.