Effects of a Proprietary Standardized Orthosiphon stamineus Ethanolic Leaf Extract on Enhancing Memory in Sprague Dawley Rats Possibly via Blockade of Adenosine A2A Receptors

The aim of the study was to explore a propriety standardized ethanolic extract from leaves of Orthosiphon stamineus Benth in improving impairments in short-term social memory in vivo, possibly via blockade of adenosine A2A receptors (A2AR). The ethanolic extract of O. stamineus leaves showed significant in vitro binding activity of A2AR with 74% inhibition at 150 μg/ml and significant A2AR antagonist activity with 98% inhibition at 300 μg/mL. A significant adenosine A1 receptor (A1R) antagonist activity with 100% inhibition was observed at 300 μg/mL. Its effect on learning and memory was assessed via social recognition task using Sprague Dawley rats whereby the ethanolic extract of O. stamineus showed significant (p < 0.001) change in recognition index (RI) at 300 mg/kg and 600 mg/kg p.o and 120 mg/kg i.p., respectively, compared to the vehicle control. In comparison, the ethanolic extract of Polygonum minus aerial parts showed small change in inflexion; however, it remained insignificant in RI at 200 mg/kg p.o. Our findings suggest that the ethanolic extract of O. stamineus leaves improves memory by reversing age-related deficits in short-term social memory and the possible involvement of adenosine A1 and adenosine A2A as a target bioactivity site in the restoration of memory.


Background
Orthosiphon stamineus Benth (Lamiaceae) is a herbaceous perennial plant, widely distributed throughout the tropical regions, especially in Southeast Asia. It is commonly known as cat's whiskers. It is also known as misai kuching in Malaysia and kumis kuching in Indonesia [1]. It is referred to as java tea and consumed as an herbal tea in Europe for urinary flushing (European Herbal Pharmacopoeia). The leaves of O. stamineus are traditionally used in South East Asia for a variety of ailments such as bladder and kidney disease (due to its strong diuretic effect), detoxification, relieving joint stiffness and inflammation including arthritis and rheumatism, gout, treating catarrh of the bladder, eliminating stones from the bladder, and treating diabetes mellitus [2,3]. Scientific studies have further reported the herb to possess anti-inflammatory [4], antioxidant [5,6], antibacterial [7], hepatoprotective [8], diuretic [9], antihypertensive [10], and hypoglycemic effects [11].
Several classes of bioactive compounds such as flavonoids, diterpenes, triterpenes, saponins, sterols organic acids, caffeic acids derivatives, chromenes, and oleanic and ursolic acid are known for O. stamineus [12][13][14][15][16]. Recent studies have emerged on the flavonoids of O. stamineus possessing antagonist activity on adenosine A 1 receptors (A1R) [17]. While the study focused more on the role of the receptors in diuretic activity, adenosine receptors in the central nervous system have also been implicated in the modulation of cognitive functions [18]. While the A1R antagonist activity has been reported in O. stamineus, A2AR antagonist activity was not. The adenosine receptors have been associated with sleep and arousal, cognition, and memory and with protecting from neuronal damage and degeneration as well as influencing neuronal maturation [19]. Endogenous adenosine is generally known to modulate cognition through the activation of adenosine A 1 receptors. Evidence is now emerging on a possible role of A 2A receptors in learning and memory [20]. The adenosine receptors A 1 and A 2A belong to the G-proteincoupled receptor family [18] and antagonist actions on these receptors produced CNS-enhancing effects. Selective blockade of A 1 and A 2A receptors were shown to facilitate learning and memory in vivo [21,22]. They might also protect against memory dysfunction shown in experimental models of aging such as Alzheimer's disease.
The social recognition test (SRT) has been used in studies with caffeine, an adenosine A 2A receptor antagonist, in reversing cognitive decline in age-related deficits in olfactory discrimination, Parkinson's disease, and attention deficit hyperactivity disorder (ADHD) [21,23]. Using the social recognition test, an adenosine A 2A receptor antagonist demonstrated the ability to reverse short-term memory loss in Spontaneously Hypertensive Rats (SHR) which have impairments across several cognitive domains such as attention, short-term memory, and spatial reference memory [20].
The social recognition test was first introduced by Thor and Holloway [24] and is based on the premise that rodents spend more time with unfamiliar juveniles than familiar ones. Memory-enhancing drugs are used in this model to investigate whether the duration of investigation is reduced when the juvenile rat is presented twice. The social recognition test in rats has become increasingly popular for the pharmaceutical industry as a tool to evaluate compounds for procognitive activity. This memory test probes shortterm recognition/working memory to investigate novel target mechanisms relevant to cognitive impairment including neuropsychiatric disorders such as dementia, Alzheimer's disease (AD), schizophrenia, and Parkinson's disease (PD). Importantly the test uses spontaneous naturalistic behavior of an adult rat when exposed to a juvenile conspecific on two occasions to access cognition, where the output measured (recognition index (RI)/ratio of investigation duration between the two sessions) involves an assessment of social exploration, strongly influenced by an olfactory component. As a result, SRT animal model was selected in this study.
Antagonists to A2AR are not the only target when seeking cognition enhancing treatment. The inhibitory effects on other target sites such as acetylcholinesterase and serotonin have shown improvement in memory and cognition. One such plant preparation shown to possess antiacetylcholinesterase activity, a neurotransmitter related to learning and memory, is the standardized extract of Ginkgo biloba. Standardised extracts of G. biloba were shown to improve memory and normalized cognitive deficits in animal models [25,26]. Meanwhile, leaves of another Malaysian herb, Polygonum hydropiper, have been reported to also possess antiacetylcholinesterase activity and recently its related species P. minus demonstrated enhanced memory in rats study using the Barnes maze test and demonstrated anticholinesterase activity [27]. The purpose of this study is to evaluate O. stamineus leaf ethanolic extract for cognition-improving benefits and adenosine A 2A receptor as a possible target. The effect is compared with G. biloba and P. minus extract, for memory improvement in an SRT animal model. stamineus leaves was dried by oven at a temperature of 40 ∘ C for 48 hours and ground into a fine powder using a lab mill (Retsch ZM200, Haan, Germany) and was extracted twice with 2 L and 1.5 L of 70% ethanol in water (v/v) using ultrasonic treatment for a period of 30 min at room temperature. The solution was separated from the remaining material. The organic solvent was removed under reduced pressure at 40 ∘ C and dried.

HPLC Analysis of O. stamineus Ethanolic Extract.
The extract was characterized using HPLC techniques based on seven known compounds of O. stamineus used as reference standards [28]. The compounds were 3 -hydroxy-4 ,5,6,7tetramethoxyflavone, sinensetin, orthosiphol B, orthosiphol A, staminol A, orthosiphonone A, and ombuin (3,3 ,5trihydroxy-4 ,7-dimethoxyflavone). HPLC analysis of the extract was performed using Agilent 1200 Liquid Chromatography (LC) with a photodiode array detector on Zorbax Eclipse XDB-C18, 4.6 × 150 mm, 5 m column. The mobile phase consisted of solvent A: water and solvent B: acetonitrile. The following gradient was used: 0-8 min, 70% A; 8-15 min, 70-53% A; 15-30 min, 53-49% A, hold for 10 min; 40-42 min, 49-0% A, hold for 4 min; 46-48 min, 0% A for final washing and equilibrium of the column for the next run. Operating conditions were set at flow rates of 1 mL/min, column temperature at 25 ∘ C, UV detection at 230 nm, and injection volume of 5 uL. The extract at the concentration of 50 mg/mL was first injected followed by the mixture of the standards. Identification of the marker compounds was achieved by comparing with retention times of reference standards and their UV spectra.

Aqueous
Extract of P. minus. 1000 g of aerial parts including stem and leaves of the plant was harvested at maturity approximately 2 months after planting and was dried by oven drying at the temperature of 40 ∘ C for 48 hours and shredded to 2 to 5 cm in size. The dried leaves were extracted according to the method described in George et al. [27]. The dried leaves were then subjected to percolation using purified water and extracted at a temperature of about 80 ∘ C with an extraction ratio of approximately 1 : 10. The extract was further filtered, concentrated using rotary evaporator with the water bath temperature of 65 ∘ C, and freeze-dried. The voucher specimen of the plant (SK 2077/12) was deposited in the Herbarium, Institute of Bioscience, UPM, Malaysia.

In Vitro Adenosine Receptors A 2 and A 1 Assays.
The adenosine A 2A receptor (A2AR) and A 1 receptor (A1R) assays were performed to determine test item's A2AR and A1R blockade activity. O. stamineus extract was tested at 15 and 150 g/mL for A 2A binding assay, and the method employed was adapted from the one described by Varani et al. [29]. Adenosine A 2A and adenosine A 1 functional assays were performed at 3,30 and 300 g/mL and the method was adapted from Paucher et al. [30] and Taylor et al. [31], respectively. Adenosine A 2A binding assay, selective adenosine A 2A , and adenosine A 1 antagonist assays were conducted by Eurofin Panlabs (previously known as Ricerca) with test catalog numbers of 200610, 300500, and 401000, respectively. Reference standards were run as an integral part of all three assays to ensure the validity of the results. The assays were performed under conditions described in Tables  1-3.

Animals.
Ninety adult male SD rats (3-month-old, 200-250 g) and juvenile male rats of the same strain (35-40day-old, 75-100 g) from the National Institute of Nutrition, Tarnaka, Hyderabad, were used as described in Table 4. The diet comprised standard pellet diet by Provimi (Nutrilab Rodent). Juvenile rats were kept in groups of ten per cage and served as social stimuli for the adult rats. The animals were maintained in a room under controlled temperature (22 ± 2 ∘ C), with relative humidity of between 50 and 70% and were subjected to a 12 h light cycle (lights on 8:00 a.m.) with    [32], and 200 mg/kg water extract of P. minus (as a direct comparison with the lower dose of the test extract) and vehicle were administered orally, 120 min before the second encounter C2.

Social Recognition Test.
Short-term social memory was assessed with the SRT described by Mondadori et al. [33]. Nine groups of rats, each consisting of 10 males, were used for the study. Adult Sprague Dawley (SD) rats were housed individually in polycarbonate cages and they were used only after at least 7 days of habituation to their new environment. The test was scored in a consistent manner in an observation room, where the rats had been habituated for at least 1 h before the beginning of the test. All juveniles were isolated in individual cages for 30 min prior to the beginning of the experiment. The SRT consisted of two successive presentations (5-10 min each) separated by a short period of time where a juvenile rat was placed in the home cage of the adult rat and the time (s) spent by the adult in investigating the juvenile (nosing, sniffing, grooming, or pawing) was recorded (C1). At the end of the first presentation, the juvenile was removed and kept in an individual cage during the delay period and reexposed to the adult rat after 120 min and time (s) spent by the adult in investigating the juvenile was recorded (C2). In this paradigm, a reduction in the investigation time during the second encounter reflects the recognition ability of the adult rat. A pretest was performed for verification that the test compounds themselves do not have effects on social investigation per se. In this experiment, a different juvenile to the one used in the first presentation was exposed to the adult rat during the second encounter, with a similar duration of social investigation time being expected. RI was calculated using the formula (RI = C2/C1) for social recognition assay. All values are expressed as means ± SEM ( equals the number of rats included in each analysis). The RI (RI = C2/C1) was calculated for social recognition assay. The data was analyzed by comparing control versus treatment and standard and changes in activity before and after treatment (C1 versus C2) and RI versus control, standard, and treatment using Student's -test by Graph Pad Prism 4.0 software.

In Vitro Adenosine A 2 Receptor (A2AR) and Adenosinse
The extract showed similar activity in A1R inhibition, with an antagonist activity at 300 g/mL where the extract displayed 100% inhibition of response induced by cyclohexyladenosine (CHA). The antagonist activity of the O. stamineus leaves ethanolic extract to adenosine A 2A and adenosine A 1 receptors suggests the biological activity of O. stamineus in an in vitro system. The IC 50 for A2AR binding activity is estimated at 60.07 g/mL and determined with nonlinear regression analysis by Inplot GraphPad Prism, San Diego, CA, computer program. The IC 50 for A1R antagonist is 95.1 g/mL ( Figure 3). The IC 50 for A2AR antagonist based on the response curve is 51.5 g/mL ( Figure 4). The Ki value for the A2AR binding assay is calculated using the Cheng-Prusoff equation (1973) and is estimated at 33.72 mM.

Social Recognition Test.
In the SRT procedure, SD rats presented a clear impairment of the juvenile recognition ability (recognition index) in comparison to control rats ( < 0.001), since control group spent as much time investigating the juvenile rat during the second encounter as they did on the first exposure. The difference between treated and control groups on juvenile recognition ability is showed with more details in Table 6, with detailed analysis of the investigation time. The investigatory behaviour of the adult SD rats was concentrated in the first 5 min of the juvenile presentation, with a significant reduction in the investigation time during the second encounter 120 min later.    of P. minus and G. biloba has shown small change in inflexion; however it remained insignificant for RI compared to vehicle, at an oral dose of 200 mg/kg and 120 mg/kg. The standard drug donepezil dosed at 3 mg/kg i.p. has shown change in inflexion but no significant change in RI as compared to vehicle control.

Discussion
The chemical constituents and the A2AR binding activity of O. stamineus extract have demonstrated that, with a single treatment of O. stamineus leaves extract after C1, the time spent in scrutinizing the same partner at a second meeting, 120 min later, is shortened. The extract-induced reduction of the exploration time can be attributed to learning of the specific information of the partner retained from the first meeting that reduced the need for new information.
The assumption that specific attributes of a particular partner were remembered is strengthened by the significant RI ( < 0.001) seen with the test extract. The ethanolic extract of O. stamineus leaves showed significant activity with 74% inhibition of A2AR at a dose of 150 g/mL. Therefore, the study suggests the possible binding of the O. stamineus extract to A2AR, attributing the social recognition task with this biological activity.
The present results demonstrate that the SD rats present a significant impairment of short-term social memory in SRT in the vehicle group as the RI of more than 1 signifies no improvement in recognition (RI should be <1). In fact, a longer time to recognize juvenile rat (C2) was observed for the vehicle group. The findings also suggest the involvement of the adenosine receptors in this response, since the acute administration of O. stamineus leaves extract reversed this social memory deficit in SD rats. Several studies have demonstrated that the selective blockade of adenosine A 1 and adenosine A 2A receptors facilitates learning and memory in rodents models [22,34]. The Ki value of the extract in this experiment was 33.72 mM. Caffeine and theophylline, another naturally occurring xanthine mainly found in tea, are nonselective AR antagonists. Their stimulating properties are associated with micromolar range affinities for the A2AR. Although caffeine and theophylline have similar in vitro affinities for the A 2A receptor, caffeine has a higher stimulating effect due to a higher brain unbound fraction wt a Ki value of 23400 nM [35]. Though caffeine derivatives possess stronger A2AR binding activities, the multicompounds that exist in Orthosiphon stamineus extract including flavonoids may have affected memory more than one way possibly also via other receptors such as the inhibition of acetylcholinesterase, Evidence-Based Complementary and Alternative Medicine 7 thereby enhancing cognition [36]. Furthermore, the extract tested in this study also possessed additional A1R antagonist activity, as previously reported in O. stamineus [17]. From the SRT, promnesic property of O. stamineus leaves extract was observed in SD rats at dose dependent manner of 300 mg/kg and 600 mg/kg p.o. and 60 mg/kg and 120 mg/kg i.p. This study revealed that the O. stamineus leaves extract (300 and 600 mg/kg, p.o.; 60 and 120 mg/kg i.p.) exerted significant activity when compared to standard donepezil (3 mg/kg i.p.) statistically reaching significance in all except at 60 mg/kg i.p. where RI was only almost significant ( < 0.0539). The O. stamineus leaves extract appears to prevent the amnesic effect of the long delay (120 minutes) where such a preventive effect may be deduced as promnesic. The extract at 120 mg/kg i.p. was comparable to 300 mg/kg and 600 mg/kg orally dosed in RI, signifying greater bioavailability when administered i.p. at only one-third of the oral dosage.
As for G. biloba extract, a 120 mg/kg oral dose failed to demonstrate significant activity in this study but in another study, a single i.p. injection of G. biloba extract at 120 mg/kg dose demonstrated improvement in recognition performances in young rats in a similar olfactory animal model study [32]. This may be due to G. biloba having a lower bioavailability when administered through nonintravenous route (as observed in this study). The herb P. minus on the other hand has been shown to possess antiacetylcholinesterase activity in a recent study [27] although in the current study improvement in recognition index was not significant. The promnesic effects are probably more apparent in a model that tests attention rather than learning and memory from olfactory cues. This is in parallel to findings by Blokland [37] where it was suggested that the role of acetylcholine in learning and memory processes was still not conclusive, rendering its role more important in attention processes. A different animal model such as Barnes maze that tests spatial learning and memory instead of memory by olfactory and social cues, such as in this study, may have been a better model to investigate P. minus [27].
Based on the mean of RI for the extracts given i.p., donepezil fared better (in terms of lower mean) than the plant extract of G. biloba at their tested dosage though the route of administration was different. A2AR activity has never been known for G. biloba though anticholinesterase activity has been reported [25] and in vivo memory improvement has been documented for G. biloba extracts [26]. Donepezil, a reversible inhibitor of cholinesterase which is clinically used for treatment of dementia, showed slightly weak (RI at < 0.0593) cognition enhancing properties at 3 mg/kg i.p. The effect of 3 mg/kg donepezil is similar to 60 mg/kg i.p. of O. stamineus extract based on the mean at C1 and having changes to RI after treatment at < 0.0593 and < 0.0539, respectively. This may be due to it being a popular acetylcholinesterase inhibitor for memory impairment which is age-related and long term such as Alzheimer and dementia. The short reaction time (120 minutes) and the use of adult but not aged rats to impart a significant effect in the RI may have contributed to the weaker response in the Donepezil group.
Neurodegenerative disease can be the result of neuronal cell death caused by oxidative stress, apoptosis, and inflammation. Apart from A2AR activity, alcohol extracts of O. stamineus leaves may possess other biological activities that are neuroprotective. They have been reported to possess antiapoptotic effects in a H 2 O 2 (a potent free radical) induced cell apoptosis [5]. The antioxidant properties of O. stamineus in addition may play a positive role in the prevention of neurogeneration caused by damaging free radicals [7]. The O. stamineus is known to contain several classes of bioactive compounds such as flavonoids, diterpenes, triterpenes, saponins, sterols organic acids, caffeic acids derivatives, chromenes, and oleanic and ursolic acid, known for [12][13][14][15][16]. Flavonoids have been shown to possess antioxidative and anti-inflammatory effects that suggest neuroprotective property [36]. Oleanic acid which has been isolated from O. stamineus has been reported to protect against neuronal death induced by beta-amyloid in cultured rat cortical neurons and improve beta-amyloid induced memory deficit in mice [38]. Ursolic acid reduced the production of proinflammatory cytokines and neurotoxic reactive oxygen species, thus possibly leading to an additional neuroprotective effect [39].
Caffeine is another example of adenosine A 2A receptor antagonist that modulates the release of different neurotransmitters in the olfactory bulb of rodents [40] known to play a role in social olfactory recognition [41]. It appears to be that O. stamineus extract behaves similarly to caffeine in improving short-term memory and alertness. O. stamineus, however, does not contain caffeine but is rich in terpenoids and flavonoids. Terpenoids from natural products such as G. biloba and Asian ginseng (Panax ginseng) are currently being investigated as potential therapeutics in Alzheimer's disease, already showing some promise [42]. Terpenoids were identified in the O. stamineus extract used, that is, orthosiphol B and orthosiphol F, staminol A, and orthosiphonone A, which makes this extract a potential candidate for further investigation in the area of cognition disorder.

Conclusion
Our findings suggest that the propriety standardized ethanolic extract of O. stamineus may reverse age-related deficits in short-term social memory and can be considered to prevent or decrease the rate of neurodegeneration. The further investigation of not only adenosinergic but also other neurotransmitters in producing improvements in cognition should be evaluated in the future. The involvement of A 1 and A 2A blockade in the social memory deficit can be further clarified in their role in the Orthosiphon stamineus effects along with selective A 1 and A 2A antagonist assayed in a social recognition tests for confirmation of target.