Phytochemical Profile and Antibacterial and Antioxidant Activities of Medicinal Plants Used by Aboriginal People of New South Wales, Australia

Aboriginal people of Australia possess a rich knowledge on the use of medicinal plants for the treatment of sores, wounds, and skin infections, ailments which impose a high global disease burden and require effective treatments. The antibacterial and antioxidant activities and phytochemical contents of extracts, obtained from eight medicinal plants used by Aboriginal people of New South Wales, Australia, for the treatment of skin related ailments, were assessed to add value to and provide an evidence-base for their traditional uses. Extracts of Acacia implexa, Acacia falcata, Cassytha glabella, Eucalyptus haemastoma, Smilax glyciphylla, Sterculia quadrifida, and Syncarpia glomulifera were evaluated. All extracts except that of S. quadrifida showed activity against sensitive and multidrug resistant strains of Staphylococcus aureus with minimum inhibitory concentration values ranging from 7.81 to 1000 μg/mL. The sap of E. haemastoma and bark of A. implexa possessed high total phenolic contents (TPC) and strong DPPH radical scavenging abilities. A positive correlation was observed between TPC and free radical scavenging ability. GC-MS analysis of the n-hexane extract of S. glomulifera identified known antimicrobial compounds. Together, these results support the traditional uses of the examined plants for the treatment of skin related ailments and infections by Aboriginal people of New South Wales, Australia.


Introduction
The Aboriginal people of Australia have over 40,000 years of knowledge of flora and fauna as sources of food, healing agents, and other resources [1]. Numerous plant species have been utilised as traditional medicines by Australian Aboriginal people [2], in particular for the topical treatment of sores, wounds, and skin infections, ailments which are especially common in Aboriginal communities [3]. For example, a retrospective review of the medical records of 99 children attending a primary healthcare centre in a remote area of the East Arnhem region in the Northern Territory of Australia found that by one year of age, 68% and 82% of the children had presented with their first case of scabies or streptococcal pyoderma (impetigo), respectively [4]. The use of plants for the treatment of such ailments indicates that they may provide extracts or pure compounds with antimicrobial or wound healing properties. However, to date, only a limited number of these plants have been investigated for their biological activities and/or chemical constituents [2,3].
Problems associated with skin related infectious diseases and chronic wounds are not limited to Indigenous communities but are serious global threats [5]. It is well known that infection rates have increased and antibiotic resistance has become a growing therapeutic problem [6,7]. In combination with bacteria being one of the most important 2 Evidence-Based Complementary and Alternative Medicine factors responsible for skin infection and delayed wound healing [8], low antioxidant levels are also associated with such diseases [9].
As part of a collaborative research program initiated upon the request of Yaegl Aboriginal people of Northern New South Wales (NSW) to help with conserving, analysing, and developing their medicinal knowledge for ecotourism and healthcare, we have ethnobotanically documented thirtytwo Yaegl medicinal plants [10] and conducted preliminary biological and phytochemical studies [11,12]. To extend this research program, we conducted a literature review in 2012 of 128 plants used as traditional medicines across NSW with regard to their distribution and habitat, documented traditional use, biological activity, and phytochemistry [3]. This review identified significant scope for further biological and chemical investigations of medicinal plants of NSW to add to the growing understanding of this resource. It also highlighted the paucity of community specific details in the published literature.
In recognition of the potential of traditional medicines for topical treatment of skin related ailments, a further literature review of medicinal plants documented in the Yaegl study [10] and NSW review [3] for these applications was conducted. This identified three plants, Hibbertia scandens (leaves), Smilax glyciphylla (leaves), and Syncarpia glomulifera (leaves), used by the Yaegl Aboriginal community, and five NSW plants, namely, Acacia falcata (bark), Acacia implexa (bark), Cassytha glabella (whole plant), Eucalyptus haemastoma (sap), and Sterculia quadrifida (leaves), for which limited or no biological and/or phytochemical studies had been undertaken. Acacia implexa (bark), Acacia falcata (bark), Eucalyptus haemastoma (sap), and Sterculia quadrifida (leaves) are reported to be used for the treatment of sores and skin complaints [3,13], Hibbertia scandens (leaves) is used for the treatment of sores and rashes [10], Cassytha glabella (whole plant) is used for bathing (topically) to relieve pain and Smilax glyciphylla (leaves) to clear skin problems, aches, and pains [3,10,13], and sap and ash from the leaves of Syncarpia glomulifera are used as an antiseptic [11] ( Table 1). C. glabella has been found to be a source of quercetin and anthocyanins [14], but no biological studies have been undertaken on this plant. S. glyciphylla possesses antioxidant activity and phenolic compounds have been isolated from its leaves [15]. S. quadrifida is reported to have moderate antifungal activity but there were no reports for phytochemical studies [16]. An antibacterial triterpenoid was isolated from the bark of S. glomulifera [17], as well as eucalyptin and 8-desmethyleucalyptin from its leaf wax [18]. The identification of essential oils from the leaves of S. glomulifera has also been undertaken [19]; however, there are no reports on the biological activity of extracts of its leaves. A. falcata, A. implexa, H. scandens, and E. haemastoma have had no reports for either biological or phytochemical studies.
In this study, 70% aqueous ethanolic extracts were prepared from the selected plants. The antibacterial activities of these extracts were determined using the MTT microdilution assay method and antioxidant activity by DPPH free radical scavenging, ABTS radical scavenging activity, and ferric reducing antioxidant power (FRAP) assay methods. Qualitative phytochemical screening and the quantification of the total phenolic, flavonoid, and tannin contents of the extracts were also undertaken. Furthermore, the n-hexane extract of S. glomulifera was chosen for gas chromatographymass spectroscopy (GC-MS) analysis, which led to the identification of several known antimicrobial and antioxidant compounds. The GPS locations of the collection sites were recorded. Voucher specimens were deposited within the IBRG Herbarium, registered with the Index Herbariorum, New York, except for S. quadrifida which was lodged with the Macquarie University Herbarium. The collected plant materials (except the sap of E. haemastoma) were thoroughly washed under running tap water and air-dried at room temperature. The dried plant materials were ground into a fine powder using a coffee grinder. The hardened sap of E. haemastoma was collected by scraping it from the trunk of the tree; the sap was then directly extracted with solvent.

Preparation of Extracts.
The powdered plant samples of A. implexa, H. scandens, S. quadrifida and S. glomulifera (0.7 L × 3, 24 h intervals), A. falcata (0.6 L × 3, 24 h intervals), C. glabella and S. glyciphylla (0.5 L × 3, 24 h intervals), and sap of E. haemastoma (0.2 L × 3, 24 h intervals) were each extracted with 70% aqueous ethanol at room temperature with occasional shaking (for plant sample amounts see Table 1). The extracts were filtered under vacuum through Whatman filter paper No. 1; then the solvent removed by evaporation using a Buchi rotary evaporator at 38 ∘ C before the crude samples were freeze-dried on a CHRIST alpha 1-4 LD plus (UK) freeze dryer. The quantities of the crude extracts obtained are given in Table 1.
Evidence-Based Complementary and Alternative Medicine 3 Hydrochloric acid (HCl), methanol, chloroform, 98% sulfuric acid, and glacial acetic acid were all analytical grade and purchased from Merck, Germany.

Phytochemical Analysis.
Phytochemical screenings for alkaloids, flavonoids, steroids, terpenoids, tannins, saponins, and anthraquinones were conducted in accordance with published methods [64][65][66]. For alkaloids, 0.02 g of extract was stirred with 2 mL of 1% HCl on a steam bath and then filtered. A few drops of Dragendorff 's reagent was used to treat 1 mL of filtrate. An orange precipitate indicated the presence of alkaloids. For flavonoids, 0.02 g of extract was dissolved in 1 mL of methanol. A chip of magnesium metal was added to the solution followed by the addition of a few drops of 11.6 M HCl. The occurrence of a magenta colour indicated the presence of flavonoids. For steroids, 0.02 g of extract was dissolved in 2 mL of chloroform and filtered (using Whatman No. 1 filter paper). 98% H 2 SO 4 was carefully added to the filtrate. A reddish brown colour at the interface indicated the presence of steroids. For terpenoids, 0.02 g of extract was dissolved in 2 mL of methanol and filtered. Acetic anhydride (1 mL) was added to the filtrate and then 2 mL of concentrated H 2 SO 4 was added carefully to the side of the tube. Formation of a reddish brown colour at the interface indicated the presence of terpenoids. For saponins, the frothing test was used. About 0.5 g of extract was mixed with 15 mL of Milli-Q water and shaken vigorously for 5 minutes. The formation of a stable froth indicated the presence of saponins. For tannins, 0.02 g of extract was dissolved in 2 mL of Milli-Q water and filtered. A few drops of 1% ferric chloride solution were added to the filtrate. Formation of a blue colour indicated the presence of tannins. Anthraquinone glycosides were detected using the Borntrager's test after hydrolysis of the extract with 10% hydrochloric acid. Chloroform was added to the hydrolysate and the contents were shaken and treated with 10% ammonia solution. The development of a pink colour indicated the presence of anthraquinone glycosides [67].

Total Phenolic Content of Extracts.
The total phenolic content was determined using Folin-Ciocalteu reagent as reported by Muanda et al. [68] with slight modification. The samples were prepared at a concentration of 1.25 mg/mL in methanol. To 250 L of extract (1.25 mg/mL in methanol), 3.5 mL of distilled water and 250 L of Folin-Ciocalteu reagent were added and the solution was allowed to stand for 5 min. Next, 1.0 mL of 20% Na 2 CO 3 solution was added to the mixture and the solution was left at room temperature for 1 h. Absorbance at 735 nm was read on a spectrophotometer as was that of a blank containing methanol. The phenolic content was calculated as gallic acid equivalent (GAE) by comparison with a calibration curve of gallic acid standard solutions (10-100 g/mL) and was expressed as mg gallic acid equivalent per gram of dry extract. Data were reported as mean ± SD for three replicates.

Total Flavonoid Content of Extracts.
Total flavonoid content was determined according to the aluminium chloride colorimetric assay with slight modification [68]. The samples were prepared at a concentration of 3.35 mg/mL in methanol. At time of 0 min, 250 L of standard solution or extract was mixed with 1 mL of Milli-Q water and 75 L of 5% NaNO 2 . After 5 min, 75 L of AlCl 3 (10%) was added to the solution and after 1 min, 500 L of NaOH (1 M) was added to the solution. Then the total solution was made up to 2.5 mL by adding Milli-Q water and mixed thoroughly. Absorbance of the mixture, pink in colour, was determined at 510 nm versus the prepared blank. The total flavonoid content was calculated as catechin equivalent by comparison to a calibration curve of catechin standard solutions (10-100 g/mL) and was expressed as mg catechin equivalent per gram of dry weight. Samples were analysed in three replications.

Total Condensed Tannin Content of Extracts.
Total condensed tannin content was determined by the method described by Michel et al. [69] with slight modification. The samples were prepared at a concentration of 1.25 mg/mL in methanol. Sample solution (50 L) was mixed with 3 mL of 4% vanillin in methanol followed by the addition of 1.5 mL of 11.6 M HCl. The well mixed solution was allowed to stand for 15 min and absorbance was measured at 500 nm against a blank. The total condensed tannin content was calculated as catechin equivalent after comparison with a calibration curve of catechin standard solutions (10-100 g/mL) and was expressed as mg catechin equivalent per gram of dry extracts. Samples were analysed in three replications.

In Vitro Antioxidant Assays
The antioxidant activities of the plant extracts were determined using the DPPH radical scavenging protocol described by Liu et al. [70]. The solutions of extracts were prepared at different concentrations (6.75-100 g/mL) in methanol. DPPH solution (50 L and 1 mM) in methanol was mixed with 200 L of sample solution and the solution mixed well by shaking before being left standing at room temperature for 30 min in the dark. The absorbance was measured at 517 nm against the blank (methanol). Ascorbic acid at the same concentrations was used as the standard. All measurements were done in triplicate. The scavenging ability of the extracts was calculated using the following equation: Abs control × 100. (1) From a plot of concentration against percentage of inhibition, a linear regression analysis was performed to determine the Evidence-Based Complementary and Alternative Medicine 5 IC 50 value (the extract concentration that could scavenge 50% of the DPPH radicals).

ABTS Radical Cation
Scavenging Activity Assay. The ABTS assay method was used as directed by Adedapo et al. [71]. A stock solution was prepared by mixing 7 mM ABTS •+ solution in water and 2.4 mM potassium persulfate solution in water in equal volumes and allowing the mixture to react for 12-16 h at room temperature in the dark so that it reached a stable oxidative state. The working solution was then prepared by diluting with methanol to an initial absorbance of 0.700 ± 0.020 (Abs control ) at 734 nm. The solution was prepared fresh for each analysis. The solutions of extracts were prepared at different concentrations (6.75-100 g/mL) in methanol. Then 1 mL of sample solution was mixed with 1 mL of ABTS •+ solution and the absorbance was measured at 734 nm after 7 min against methanol as the blank. All measurements were done in triplicate. Trolox was used as a positive control. The percentage of scavenging inhibition capacity of ABTS •+ of the extract was calculated using the following formula: IC 50 values of the plant extracts were also determined for ABTS •+ .

Ferric Reducing Antioxidant Power (FRAP) Assay.
The FRAP assay was carried out by following the method described by Wang et al. [72]. The FRAP reagent included 300 mM acetate buffer   [73]. A solution of each sample (10 mg/mL) in 20% aqueous DMSO along with that of a suitable antibiotic (1 mg/mL, vancomycin for Gram-positive strains and gentamycin for Gram-negative strains) was prepared and serially diluted to give a final plant sample concentration of 2-1000 g/mL and antibiotic concentration of 0.05 to 100 g/mL in 96-well clear bottom microtitre plates. Test samples (20 L) were inoculated with 175 L of microbial culture ( 600 = 0.08 diluted 100-fold in MH broth); a sterile broth control was included. A 20% DMSO control was also included and the plates were incubated at 37 ∘ C. After 18 hrs of incubation 5 L of a methanolic solution (5 mg/mL) of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) was added to each well and the plates further incubated at 37 ∘ C for 1 h to determine the MIC. MTT was used as an indicator of where microbial growth reduced the yellow tetrazolium bromide to a violet formazan. MIC was described as the lowest concentration of the test compounds that inhibited visible growth of the microorganisms (the last well showing no colour change of MTT from yellow to blue).

GC-MS Analysis of n-Hexane
Extract of Syncarpia glomulifera. The 70% aqueous ethanol extract (6.0 g) of S. glomulifera leaves was partitioned with n-hexane (50 mL × 3), dichloromethane (50 mL × 3), ethyl acetate (50 mL × 3), n-butanol (50 mL × 3), and water (50 mL × 3) to give 950 mg, 1.0 g, 540 mg, 1.6 g, and 750 mg of each partition, respectively. The partitions were tested for their antibacterial activity against sensitive and resistant strains of S. aureus, E. coli, and P. aeruginosa. The n-hexane extract was selected for GC-MS analysis by gas liquid chromatography (GLC) and gas chromatography-mass spectrometry (GC-MS). GLC was carried out on a BP-20 column (60 m × 0.25 mm × 0.25 m). The temperature program was 50 ∘ C (5 min) to 220 ∘ C (15 min) at 3 ∘ C/min with helium as the carrier gas. The temperature of the injector and that of detector were both set at 220 ∘ C. The BP-20 column (30 m × 0.35 mm × 0.25 m), programmed from 35 ∘ C to 220 ∘ C at 3 ∘ C/min, was used for GC-MS with helium as the carrier gas and an injector temperature of 220 ∘ C for the column. Mass spectra were recorded in electron impact (EI) mode at 70 eV, scanning from 41 to 450 m/z. Compounds were identified by their identical GC retention times and retention indices relative to n-alkanes and by comparison of their mass spectra with either pure standards or published spectra in the NIST GC-MS library and those in the literature [56][57][58][59][60][61].   analysis. Values with < 0.05 and < 0.01 were considered statistically significant and very significant, respectively. The experimental results were compared by paired -test (two sided).

Phytochemical Screening.
Qualitative phytochemical tests of the 70% aqueous ethanol extracts of the eight plants showed the presence of alkaloids, terpenoids, flavonoids, steroids, saponins, tannins, and anthraquinones ( Table 2). These classes of phytochemicals are known to possess a variety of biological activities including antimicrobial, antioxidant, anti-inflammatory, antiplasmodial, and anticancer activities [74][75][76][77][78][79][80][81][82][83][84]. These findings may partially justify the traditional use of the examined plants in the treatment of wound and skin infections and free radical mediated diseases and indicate that they may serve as a source of bioactive compounds against these illnesses.

Total Phenol, Flavonoid, and Condensed Tannin Contents.
Phenolic compounds are effective hydrogen donors, making them good antioxidants [80]. Plant derived polyphenolic flavonoids are also well known to exhibit antioxidant activity. Flavonoids reduce free radicals by quenching, upregulating, or protecting antioxidant defences and chelating radical intermediate compounds [85]. It is also reported that tannins are 15-30 times more effective in quenching peroxyradicals than simple phenolics [86]. The phenolics and polyphenols are one of the largest groups of secondary metabolites to have exhibited antimicrobial activity [87]. The site(s) and number of phenol groups are thought to be related to their relative toxicity to microorganisms, with evidence that increased hydroxylation results in increased toxicity [88]. Naturally occurring plant flavonoids have also been reported to possess antimicrobial activities [79,89,90]. The variation in the antibacterial activity of flavonoids is known to be related to their chemical structure, especially in regard to the number and positions of methoxy and phenolic groups within their structures [91][92][93]. The antimicrobial effects of tannins have also been widely recognised [94][95][96]. Therefore, the total phenolic, flavonoid, and condensed tannin contents of the eight plant extracts were examined to see if their traditional uses for the treatment of skin related ailments could be linked to the presence of these classes of compounds.
The results showed that the amount of total phenolic, flavonoid, and condensed tannin contents differed significantly ( < 0.05) among the extracts of the tested medicinal plants ( Table 3, Figure 1). The total phenolic contents were determined as mg GAE/g extract on comparison with a standard gallic acid graph. Three extracts showed very high phenolic contents (>400 mg GAE/g): E. haemastoma, A. implexa, and A. falcata with values of 656.2 ± 5.1, 486.7 ± 9.9, and 451.7 ± 1.3 mg GAE/g of extract, respectively.   C. glabella and S. glyciphylla showed reasonable phenolic contents (>200.0 mg GAE/g) of 275.5 ± 8.6 and 243.5 ± 5.9 mg GAE/g of extract. S. quadrifida showed the lowest phenolic content at 52.5 ± 0.6 mg GAE/g extract. The total flavonoid content was determined as mg CE/g extract after comparison with a catechin standard graph. The highest total flavonoid content was identified for A. falcata at 183.3 ± 6.0 mg CE/g of extract and the lowest for S. glomulifera at 58.0 ± 2.2 mg CE/g of extract. The total condensed tannin content was evaluated as mg CE/g of extract after comparison with a catechin standard graph. E. haemastoma showed the highest condensed tannin content at 106.0 ± 5.3 mg CE/g of extract and S. quadrifida the lowest at 9.4 ± 2.0 mg CE/g of extract. The results revealed that the level of phenolic compounds and condensed tannins was the highest in the 70% ethanolic extracts from the sap of E. haemastoma and bark of A. falcata and A. implexa. These results were significantly higher than that of the leaves of the other plants investigated.

DPPH Radical Scavenging Activity.
The results of the free radical scavenging activity of the extracts are shown in Table 4. The dose-response curves of the DPPH radical scavenging activities of the eight plant extracts were compared with that of ascorbic acid (Figure 2). In the DPPH assay, all extracts examined except for that of S. quadrifida showed radical scavenging activity in a concentration dependent manner and were significantly different ( < 0.01). This result agreed with an earlier report by Motalleb et al. [97] that showed that the scavenging effects on the DPPH radical increase sharply with increasing concentration of the samples and standards. The highest antioxidant activity was obtained for the extract of E. haemastoma (IC 50 52.0 ± 1.2 g/mL and standard ascorbic acid IC 50 71.6 ± 1.0 g/mL).

ABTS
•+ Scavenging Activity. The antioxidant activities of the plant extracts towards ABTS •+ were also determined (Table 4, Figure 2). All extracts showed the ability to neutralise the radical cation ABTS •+ , with significant differences at < 0.01. The highest activity was obtained for the E. haemastoma extract with IC 50 value of 61.7 ± 0.5 g/mL, followed by A. implexa and A. falcata with IC 50 values of 107.1 ± 1.4 g/mL and 111.5 ± 0.9 g/mL, respectively. These extracts could be seen to be rapid and effective scavengers of the ABTS •+ radical ( Figure 2) and their activities were comparable with that of Trolox.

Ferric Reducing Antioxidant Power (FRAP) Assay.
The FRAP assay was used to evaluate the antioxidant properties of the extracts based on their ability to reduce ferric (III) to ferrous (II). The results obtained from the extracts (Table 4) were significantly different ( < 0.01). For this assay it was also found that the extract of E. haemastoma provided the highest antioxidant activity with a FRAP value of 6189 ± 9.5 mol Trolox equivalent/g, followed by A. implexa and A. falcata with FRAP values of 2913 ± 6.8 and 1991 ± 2.7 mol Trolox equivalent/g, respectively.
Based on the results of all three assays, it can be seen that the sap extract of E. haemastoma and bark extracts of A. implexa and A. falcata possess the strongest free radical scavenging activities and reducing capacities of all the plant  extracts analysed, indicating that they may be useful for treating free radical-related diseases. The scavenging of the ABTS •+ radical by the extracts was found to be higher than that for the DPPH radical. It is well known that the antioxidant activity of a plant extract largely depends on both its composition and the test system [72].

Correlation between the Total Phenolic and Flavonoid
Contents and Antioxidant Activities. Table 5 shows the correlations (linear regression coefficients, 2 ) between the total phenolic contents (TPC) and total flavonoid contents (TFC) and the antioxidant assay results for the plant extracts. All the antioxidant assay results showed very good correlation ( 2 > 0.9) with the TPC and TFC values except for that of S. quadrifida, which showed poor correlation between ABTS and TFC ( 2 = 0.7953). The significant correlations between the antioxidant properties and TPCs and TFCs of the extracts may indicate that the phenolic and flavonoid type compounds contained within the plant extracts are the major contributors to their antioxidant properties.  Table 6. None of the extracts showed activity against the Gram-negative bacterial strains, even at a concentration of 1 mg/mL. Seven of the eight extracts showed activity against the sensitive and/or resistant strains of S. aureus at MIC values of ≤1 mg/mL, except for S. quadrifida which did not show any activity even at a concentration of 1 mg/mL.
According to Ríos and Recio [98] extracts possessing an MIC value equalling or less than 1000 g/mL are considered to be active and worthy of further investigation. The S. glomulifera extract showed the greatest activity against sensitive and resistant strains of S. aureus with MIC values of 7.81 g/mL against all three strains, followed by E. haemastoma and A. implexa with MIC values of 62.5 g/mL and 125 g/mL, respectively, against the sensitive strain of S. aureus.
It is well known that phenolic compounds present in plant extracts play an important role in their antimicrobial effects [99]. Phytochemical screening of the extracts in this study showed that E. haemastoma, A. implexa, and A. falcata possess a high content of phenolic compounds. It has also been reported that the active constituents in A. implexa and A. falcata include tannins [99]. Therefore, it can be inferred that the antibacterial activities of these three plants may be due, at least in part, to their high phenolic and tannin contents. This is the first report of the antibacterial activities of all of these eight medicinal plants against sensitive and resistant strains of S. aureus. The promising antibacterial activities of the extracts provides preliminary support for the traditional uses of these plants for the treatment of skin and wound infections.  The extract of S. glomulifera leaves showed the highest antibacterial activity with MIC values of 7.81 g/mL against the methicillin sensitive, methicillin resistant, and multidrug resistant strains of S. aureus. Therefore, this extract was chosen for further investigation. The crude 70% aqueous ethanol extract was partitioned with n-hexane, dichloromethane, ethyl acetate, n-butanol, and water and the partitions tested for their antibacterial activity against sensitive and resistant strains of S. aureus, E. coli, and P. aeruginosa (Table 7). Among the five partitions, the n-hexane extract showed the greatest antibacterial activities against sensitive and resistant strains of S. aureus with MIC values of 7.81 g/mL against all three strains. None of the extracts showed antibacterial activity against P. aeruginosa, and only the n-hexane partition showed activity against E. coli at a concentration of 1 mg/mL. As the nhexane extract showed the greatest activity, it was chosen for GC-MS analysis to further explore its chemical constituents.

Conclusion
Our study has shown that extracts of E. haemastoma, A. implexa, A. falcata, and S. glomulifera contain antioxidant and antibacterial compounds. The highest in vitro antioxidant activity of the plant extracts was found for E. haemastoma, with results comparable with that of the standard compound, ascorbic acid. S. glomulifera and E. haemastoma presented the best antibacterial activities against methicillin sensitive, methicillin resistant, and multidrug resistant strains of S. aureus, with MIC values between 7.81 and 125 g/mL. GC-MS analysis of the n-hexane extract of S. glomulifera revealed the presence of antioxidant and antibacterial compounds. Thus, the results of this study support the use of these plants as traditional medicines for the treatment of skin related ailments including sores, wounds, and skin infections by New South Wales Aboriginal people.