Two Traditional Chinese Medicines Curcumae Radix and Curcumae Rhizoma: An Ethnopharmacology, Phytochemistry, and Pharmacology Review

Curcumae Rhizoma, known as Ezhu (Chinese: 莪术), and Curcumae Radix, known as Yujin (Chinese: 郁金), are different plant parts coming from three same species according to China Pharmacopoeia. Actually, they are used in different ways in TCM clinical treatment. Curcumae Rhizoma is mainly used as antitumor drug, while Curcumae Radix has been used as antidepressant and cholagogue. Curcumae Rhizoma and Curcumae Radix are confused in variety and source, even in clinical trials by some nonprofessional workers. So it is important for us to make them clear. This review is aimed at summarizing the ethnopharmacology, phytochemical, and pharmacological differences between Curcumae Radix and Curcumae Rhizoma by SciFinder, CNKI, and so on, to use them exactly and clearly. Further studies on Curcumae Rhizoma and Curcumae Radix can lead to the development of new drugs and therapeutics for various diseases on the basis of the TCM theory.


Introduction
Curcumae Rhizoma (Chinese: 莪 术) and Curcumae Radix (Chinese: 郁金) are two Chinese medicines used commonly in both traditional treatment and modern clinical care. The Chinese Pharmacopoeia recorded that Curcumae Radix should be the dry radix of Curcuma wenyujin Y. H. Chen and C. Ling, C. longa L., C. kwangsiensis S. G. Lee, and C. phaeocaulis Valeton. And Curcumae Rhizoma should be the dry rhizomes derived from the above-mentioned species except C. longa L [1]. They are similar in source but different in medicinal parts.
It is recorded in the TCM ancient books that their flavors are both pungent and bitter. The nature of Curcumae Rhizoma is warm and attributive to the liver and spleen meridians, while Curcumae Radix is cold and attributive to the liver, heart, and lung meridians.
Curcumae Rhizoma and Curcumae Radix have their respective priorities on TCM efficacy. Curcumae Radix is particularly effective in activating qi for resolving stagnation (行气解郁), clearing heart fire and cooling blood (清心凉 血), together with normalizing gallbladder to cure jaundice (利胆退黄). TCM experts mainly used it to cure illnesses like jaundice, cholelithiasis caused by dampness-heat of liver and gallbladder (湿热黄疸症), and so on. Curcumae Rhizoma is good at treating the syndrome of food retention (食积症) and accumulation of extravasated blood (血积症). In general, they are both effective in promoting blood circulation for alleviating pain (活 血 止 痛) and activating qi to resolve stagnation (行气消瘀), whereas on exerting drug efficacy, Curcumae Rhizoma is stronger than Curcumae Radix on the ability of invigorating the circulation of blood (活 血). On this account, it is forbidden to be used in menstruation and pregnancy.
Most recent pharmacological researches on Curcumae Rhizoma laid emphasis on the anticancer effect particularly. Curcumae Rhizoma has been developed into a variety of formulations and used in clinical treatment. About Curcumae

Pharmacological Activities
The pharmacological actions of Curcumae Rhizoma and Curcumae Radix have gained much attention. Generally speaking, Curcumae Rhizoma has effects in antitumor, antiplatelet aggregation and antithrombosis, hepatoprotective, antioxidant, antimicrobial, and antiviral activity, and so forth. Among these, antitumor activity is most widely and emphatically studied [59,[62][63][64]. It played a significant role in the treatment of cancer and tumor in clinic [65,66]. Curcumae Radix also owns its unique priorities. In general, Curcumae Radix is mainly used as hepatic protectant and choleretic drug. In recent years, it has been used for treating depressive disorder with marked curative effect [67]. There are some relations between traditional effects and modern pharmacological. For example, Curcumae Rhizoma, the antitumor activity, and antithrombosis activity have been convinced to be relevant to the traditional effect "activating qi and breaking blood stasis." 4.1. Antitumor Activity. Nowadays, the antitumor effect of the Curcuma species has been widely studied. And they are focused on two varieties C. wenyujin and C. longa, which are carried out not only in vivo but also in vitro. In summary, the mechanism of the antitumor activity of Curcuma species was mainly concentrated on the cytotoxicity and cell apoptosisinduced.
Curcumae Rhizoma is widely used in the traditional Chinese medicine whose essential oils are widely applied in the treatment of tumor in China. And the bioactive compounds in Curcumae Rhizoma can generally be divided into two categories: volatile and nonvolatile components [12]. Among the volatile compounds, mostly are monoterpenoids and sesquiterpenoids. Particularly, -elemene is the most widely studied component. It has been proved to possess broadspectrum antitumor activity and effective treatment on the several type tumors [65][66][67][68]. As for nonvolatile compounds, curcumin and polysaccharides took up a large proportion. Similar to the former, they can also cure many kinds of tumor effectively. The antitumor effects of these terpenoids are found related to the retardation of cell cycle arrest, the induction of apoptosis, and the inhibition of metastasis or tissue invasion [12]. As a kind of acknowledged main effective constituents, people found that -elemene has the ability to inhibit the activity of ovarian cancer cells [65]. And the inhibition of -elemene-induced cancer cell proliferation is mainly due to the apoptotic cell death and cell cycle arrest [68,69]. Further study was conducted to clarify that -elemene extracted from C. wenyujin could antagonize glioblastoma cells by inducing apoptosis [13]. And this active compound was found to exert the effects inhibiting the growth of H 22 tumor cells time-and dose-dependently. It is worth noting that -elemene affects the expression of histone H 1 only at the protein level but it is not involved in regulation at the gene level [70].
There are also some other active chemical compounds of Curcumae Rhizoma. Furanodiene, germacrone, furanodiene, and 13-hydroxygermacrone, sesquiterpenes extracted from the essential oil of Curcumae Rhizoma (C. wenyujin), were shown to possess the inhibiting UVB-induced upregulation of the mRNA and protein expression levels of MMP-1, MMP-2, and MMP-3 in human keratinocytes [71]. It is reported that its essential oil extract has the ability to inhibit tumor growth whose treatment resulted in apoptosis in Hela cells [3].
The polysaccharides extracted from Curcumae Rhizoma (C. kwangsiensis) were demonstrated to significantly inhibit the proliferation of CNE-2 cells, which was possible through the induction of apoptosis mediated by attenuating Bcl-2 expression and promoting p53 expression [72]. And curcumol was evidenced to have an effect in the apoptosis in SGC-7901 [63].
Generally speaking, the amount of reports on Curcumae Radix is somehow less compared to Curcumae Rhizoma. Curcumae Radix confirmed that it could inhibit the appearance of the tumor on the colonic epithelium and chemical carcinogenesis in lots of clinical practice study [73]. An extracted compound, curcumrinol C, demonstrated that it could induce tumor cells apoptosis. And this result was associated with the increase on the expression of Caspase-9, Caspase-3, Caspase-7, and PARP (89 KD) [62]. By using MTT method, diterpenoid from Curcumae Radix was investigated to have inhibitory effects on the growth of human gastric cancer cell SGC-7901, and the mechanism might be related to the apoptosis executive protein Caspase-3 activated by regulating p65 via p38MAPK [74]. And by using RT-PCR method, the same result was obtained, but the mechanism was associated with the downregulation of VEGF expression level [64].
Curcumin extracted from C. longa not only has strong cytotoxic activity but also can inhibit cells by inducing apoptosis. For example, in a study, Tca8113 and Tb cells were treated with different concentrations of curcumin (6.25, 12.50, 25.00, and 50.00 mol/L), respectively, for 24 hours. The curcumin can notably inhibit proliferation and induce apoptosis of Tca8113 and Tb cells of human oral squamous cell carcinoma [75]. Now, a lot of work had been focused on 8 Evidence-Based Complementary and Alternative Medicine the antitumor activity of curcumin. Curcumin can inhibit a variety of tumor cells' growth and prevent from skin cancer, gastric cancer, adenocarcinoma of duodenum, colon cancer, and breast cancer of rodent induced by chemical and radioactive materials. It can also significantly reduce the number and the mass of tumors [76]. Curcumin is an acknowledged active component which is a scavenger of reactive oxygen species and has properties in skin and forestomach carcinogenesis and various pharmaceutical applications [77]. Moreover, the half inhibitory concentration, IC 50 , of the extract named curcuminol E from the Curcumae Radix (C. wenyujin) on HL-60 was 4.2 mg/L and K562 being equal to 2.7 mg/L [59]. Curcumae Rhizoma showed different mechanism of the antitumor activity. For example, fractionated extracts of C. wenyujin (Curcumae Rhizoma or Curcumae Radix not mentioned in original literature) were tested for their potential to modulate the MDR phenotype and function of P-gp in MCF-7/ADR and A549/Taxol cells in vitro [78].

Antiplatelet Aggregation and Antithrombosis Activities.
It is reported that curdione isolated from the essential oil of Curcumae Rhizoma (C. wenyujin) using the silica gel column chromatography method has a principal and active inhibitory effect on human platelet aggregation in vitro and in vivo testing. Specifically, we found that curdione could preferentially inhibit PAF-and thrombin-induced human platelet aggregation in a concentration-dependent manner (IC 50 : 60-80 M). But its inhibitory activity on ADP-and AA-induced platelet aggregation was very weak in the in vitro test. Also, curdione played an important part in the inhibition of the P-selectin expression, the increase in cAMP levels, and the attenuation of intracellular Ca 2+ mobilization in PAFactivated platelets. Moreover, curdione showed significant antithrombotic activity in vivo by testing in a tail thrombosis model [79]. Another compound, -elemene extracted from the Curcumae Rhizoma (C. wenyujin), could extend coagulation time in normal mice in a dose-dependent manner. It means that, along with the increase of dosage, antithrombosis action by -elemene was increased gradually. It played a role of antithrombosis activity by inhibiting platelet aggregation, releasing TXA 2 , and decreasing the production of platelet [80]. In another study, by using photo chemical reaction,elemene also proved that it may contribute to the prevention of thrombosis [81]. Moreover, in an experimental study, the Curcumae Rhizoma (C. phaeocaulis) revealed an active effect on inhibiting platelet aggregation and prolonging time of mice analgesic. And the products with vinegar are more powerful [82].
There are no so many researches which could prove the activity of Curcumae Radix. The polysaccharides from Curcumae Radix (C. kwangsiensis) held anticoagulant activities confirmed by prolonging significantly the clotting time (CT), thrombin time (TT), and activated partial thromboplastin time (APTT) of mice in in vivo study [83].

Hepatoprotective Activity.
Through summarizing literatures, the author found that there are two extracts which have the antihepatotoxic activity. One, ethanolic extract and essential oil, is mostly extracted from C. kwangsiensis or C.
wenyujin. For example, the volatile oil of C. wenyujin can reduce the activity of S-GDT, have a certain repair effect on liver function, and correct A/G proportion inversion caused by hepatitis. It also can protect liver cells and promote the regeneration of liver tissues by the clinical observation and animal experiment [84]. The other, mainly curcumin, is extracted from C. longa.
Essential oils extracted from Curcumae Rhizoma (C. kwangsiensis) are effective in protecting liver from the damage induced by CCl 4 , TAA significantly, and it may be due to descending transaminase function and drug enzymeinduced function [85].
The model mice of acute hepatic injury were established with intraperitoneal injecting 0.2% CCl 4 0.2 mL/10 mg to observe the activities of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in hepatic tissue. It convinced that Curcumae Radix could protect the liver from acute hepatic injured induced with CCl 4 in mice [86]. Curcumin has powerful protective effects against acute liver injury induced by CCl 4 , D-GaLN, BCG, and LPS. And it might have therapeutical effect on liver fibrosis [87]. Furthermore, the eight sesquiterpenes, furanodiene, curdione, neocurdione, dehydrocurdione, germacrone, 13hydroxygermacrone, curcumenol, and curcumenone, also showed a protective effect against D-GaLN/tumor necrosis factor-alpha-induced liver injury in [88]. As for hepatic toxicity caused by some things in life like alcohol (drink), paracetamol (drug) was confirmed experimentally to be treated by curcumin. Curcumin can improve antioxidant enzyme activity and protect liver from ethanol-induced oxidative stress damage.

Antioxidant
Activity. The two medicines have antioxidant effects through different mechanisms. For example, it is observed that the medium and the highest dose of aqueous extracted from Curcumae Rhizoma (C. kwangsiensis) could increase the CAT and GSH-Px activities in cytosols in liver of rats which are recognized as the main component part of antioxidant system in the body. It is indicated that the extracts at a large dose could increase the antioxidative activity of the rats' liver [89]. The rise in free radicals level is thought to be related to the increase in TC, LDL-c, and TG and fall of HDL-c. And in a study, Curcumae Rhizoma (C. kwangsiensis) is proved to contain natural antioxidants, polysaccharides in which isoflavones and a class of phytochemicals can be found. It revealed that the polysaccharides of water extract possessed strong free radicals scavenging activities.
It can bring down the elevated levels of TC, LDL, VLDLc, and TG in high-fat animals on protecting against oxidative injury induced by high-fat diet treatment [90]. Moreover, Curcumae Rhizoma oil could elevate the antioxidant enzymes in blood such as SOD, GSH-Px, and MDA [91].
As for Curcumae Radix, the extract of the Curcumae Radix (C. wenyujin) could reduce the lipid peroxidation production by protecting and improving the antioxidant activity like CuZn-SOD, Mn-SOD, GSH-Px, and CAT in rats which were damaged by radiation [92]. There are many reports on the antioxidative activity of C. longa. The PC-OOH level rising was considered as an oxidative stress marker Evidence-Based Complementary and Alternative Medicine 9 caused by alcohol consumption. And curcumin has the power to lower the PC-OOH level in liver significantly [93]. Also, it has the DPPH radical scavenging effects with IC 50 values of 2.8 M [94]. Curcumin can also increase the activity of SOD, which is considered to be associated with induction or progression of many diseases closely [94][95][96].

Anti-Inflammatory Effect.
There are many reports indicating anti-inflammatory effect of Curcumae Radix. The ethanol extract and water extract of Curcumae Radix (C. kwangsiensis) possessed protective effect against dimethylbenzene and ethylic acid induced inflammation in the pinna swelling model of mice and the cotton ball granuloma model of mice. It was associated with producing significant inhibitory effects on the increased blood capillary permeability and the swollen and hyperplastic granulation tissue in rats [6]. By using hot plate test, xylene-induced mouse ear edema test, and chemical irritation-induced writhing test, we found that the ethyl acetate extract of Curcumae Radix (C. wenyujin) had the analgesic and anti-inflammatory effects. The extract had the analgesic effects improved with increased-dosages by physical and chemical stimulated. It had obviously inhibited inflammatory edema and celiac capillary permeability, and it could also reduce the level of TNF-. The mechanisms may be inhibited by the level of TNF- [7]. Similarly, another study also showed that it had stronger anti-inflammatory and antinociceptive effects [9]. Furthermore, curcumin from C. longa attenuated the development of allergic airway inflammation and hyperresponsiveness, possibly through inhibition of NF-ΚB activation in the asthmatic lung tissue [97]. In addition, polysaccharide and water extract of C. longa might be responsible for anti-inflammatory action by downregulating the PGE2 and IL-12 levels in LPS stimulated mouse splenocytes [98].
The Curcumae Rhizoma also has the anti-inflammatory activity. Sun et al. found that the derived sesquiterpenoids named furanodiene and curzerene extracted from Curcumae Rhizoma (C. wenyujin) have significant inhibition effect on the TNF-factor excreted by THP-1 cells [99].

Antimicrobial and Antiviral Activity. Essential oils from
Curcumae Rhizoma (C. kwangsiensis, C. phaeocaulis) have stronger and broad-spectrum antagonistic activity on 6 kinds of fungi. The hyphal cell wall disappeared and protoplast was dissolved by observation on electron microscope, which indicated that Curcumae Rhizoma (C. kwangsiensis, C. phaeocaulis) was the promising for biological pesticide [100].
Essential oils extracted from the C. wenyujin, which come from rhizomes and radixes, were also investigated to have antimicrobial activity on the selected microorganisms including two bacterial strains (Propionibacterium acnes and Staphylococcus aureus) and one fungal strain (Malassezia furfur). It showed only weak inhibitory activity against Malassezia furfur. Only a weak inhibitory effect was observed for oil from radix of C. wenyujin. Moreover, the study proved that the monoterpenoids have a higher inhibition activity than the sesquiterpenoids do [10]. Another study demonstrated that sesquiterpenoids from C. wenyujin showed significant in vitro antiviral activity against the influenza virus A (A/H1N1/Guangdong/219/2006) cells with IC 50 values ranged from 6.80 to 39.97 M and SI values ranged from 6.35 to 37.25 by using cytopathic effect (CPE) reduction assay and measuring cytotoxicity in parallel with the determination of antiviral activity [4]. Furthermore, in recent years, many studies have begun to make directional biotransformation of the extract from Curcuma. For example, the directional biotransformation of curcumol by Penicillium janthinellum was studied. And the compound transformed from curcumol could have a significant antivirus activity on parainfluenza virus, respiratory spore virus, and herpes simplex virus (HSV) type I in vitro [101].

The Effect on the Stomach and the Bone.
The curcumin of C. longa ethanol extracted was proved to have protective effects on stomach by making the animal gastric ulcer models with cold water stress of mouse, with alcohol induction of mouse, and with delegation at gastric pylorus cardiac orifice of rice. And the animals which were randomized into different five groups were given different dosage of curcumin. The result showed that curcumin has protective effects on the gastric mucosa injury whose mechanism was possibly related to inhibition of peroxidation reaction [102]. Similarly, by the animal experiment, the other study revealed that there was delay in gastric emptying following intragastric administration of curcumin. And it could be explained by the NOS inhibitory action [103].
Curcumin extracted from C. Longa also has favorable applied value on the clinical research. In the report by Chen et al., curcumin whose dosage was up to 8000 mg/d and whose time given to the patient was 13 months has no toxicity on human [104].
Curcumin may affect the skeletal system. At the cellular level, curcumin modulates important molecular targets: transcription factors, enzymes, cell cycle proteins, cytokines, receptors, and cell surface adhesion molecules, because many curcumin targets which were mentioned above participate in the regulation of bone remodeling. And its mechanism partially related to the inhibition of NO production which somehow is similar to what is mentioned above.
In a word, as a matter of fact, curcumin could facilitate the attenuation of tissue and cellular injury in the liver, heart, kidney, brain, and bone, which are proved to be induced by oxidation or inflammation [105].

Other Pharmacological Activities.
What is more, Curcuma has been investigated to exert other pharmacological activities. In vivo study showed that four Curcuma drugs derived from C. longa, C. kwangsiensis, C. phaeocaulis, and C. wenyujin had an effect on the blood vessel. The result demonstrated that the extracts curcumin and sesquiterpenes showed NO-independent relaxation effects with almost the same intensities, while polysaccharides, in contrast, showed contraction effects [106].
Polysaccharides from rhizome, stem, and leaf of C. kwangsiensis have vitro fibrinolytic activities by using fractional precipitation method and fibrin plate method [107].
Curcumae Radix has antidepressant effect. And the active parts are in petrol fraction and ethyl acetate fraction [67].
Besides, Curcumae Radix has cholagogue activity [108]. Moreover, curcumin has a potent protective effect against the testicular toxicity [109]. Also, curcumin has a distinct effect to reduce the blood lipid. The animal experiments showed that curcumin could effectively reduce the total cholesterol and triacylglycerol of the serum and liver tissues and increase the activity of the related enzymes involved in lipid metabolism in plasma, while the antihyperlipidaemic effect particularly can be shown in the result of reducing blood lipid and raising the level of HDL in serum [110].

Drug Development.
In the present clinical practice, those classic prescriptions have been employed in a more extensive and intensive way. Nowadays, many prescriptions ( Table 2) were applied into the clinic in the forms of pill, granule, capsule, and injection for the sake of convenience. Similarly, their effects evidenced by clinical reports are still beyond all doubt. Zedoary turmeric oil and glucose injection was used to treat 85 patients with acute upper respiratory infection and 83.5% was effective [111]. Curcuma oil injection can be also effective and shorten the duration of the main symptoms on the treatment of acute upper respiratory tract infection [112]. As for Curcumae Radix, for example, Curcumae Radix injection has been proved to be effective in curing icteric virus hepatitis [113].

Chemical Constituents of Curcumae Rhizoma and Curcumae Radix
To date, the chemical constituents from Curcuma Rhizoma and Curcuma Radix reported about 207 compounds, mostly assigned to sesquiterpenes and diarylheptanoids, also including alkaloids, polysaccharides, and some other types. The two main types of compounds are considered to be their bioactive chemical constituents. Sesquiterpenes, also the main constituents on essential oils, are responsible for their extraordinary anticancer, anti-inflammatory, antiviral, neuroprotective, and antithrombotic effect [10,85,110]. Diarylheptanoids, which also possess various pharmacologic activities, such as curcumin, demethoxycurcumin, and bisdemethoxycurcumin, are the major nonvolatile compounds in Curcumae Rhizoma and Curcumae Radix [114]. In this section, we summarize and classify all of the reported constituents in Curcumae Rhizoma and Curcumae Radix from four species (C. wenyujin, C. longa, C. kwangsiensis, and C. phaeocaulis) that Chinese Pharmacopoeia regulated. Their structures are shown in the following, while the corresponding plant sources and references are collected in Table 3. (Figure 2). Sesquiterpenes are defined as 15-carbon compounds formed from 3 isoprenoid units. Sesquiterpenes, extremely diverse, heterogeneous, and large group of natural compounds, are widely used in folk medicines, health-supporting preparations, and cosmetics [115]. 133 [118] isolated a sesquiterpene named curdione firstly whose nuclear parent was a ten-membered ring. Such a large ring is flexible and easy to distort. Most compounds with this nuclear parent have many conformations under normal temperature which attaches great degree of difficulty to identify its absolute configuration for researchers. Later they achieved the aim by using the single crystal diffraction method combined with the calculation of molecular energy and the molecular model. See compounds 54-84.

Bisaborane Type.
In 1987, Uehara et al. [119] isolated 3 chemical compounds from C. xanthorrhiza and identified them, respectively, as bisacurone, bisacumol, and bisacurol. C. xanthorrhiza is used in Indonesian folk medicine with many activities, which may be used as Curcumae Rhizoma, while it is not included in official species [120]. Since then, 15 sesquiterpenes (85-99) of this kind had been reported by other studies one after another.

Elemane
Type. In 1968, zedoarone was isolated from C. zedoaria Roscoe by Hikino et al. [122]. Later, other researchers found 12 sesquiterpenes (112-121) of this kind from Curcumae Rhizoma and Curcumae Radix. (Figure 3). There is another important active ingredient named diarylheptanoids, also called curcumin components whose pharmacological activity has been one of the research hotspots in the field of medicine in Curcumae Rhizoma. Among them, the curcumin has been regarded as the third generations of anticancer drug to be studied by the National Cancer Institute. The nuclear parent of this kind is linear diarylheptanoids which can be divided   Radix a C. kwangsiensis [15] 2 Curcumenol Rhizome C. phaeocaulis [16] Radix C. wenyujin [17] 3
In conclusion, in comparison to Curcumae Rhizoma, the number of studies on Curcumae Radix is less. There are only 10 articles investigating chemistry of Curcumae Radix.
The first study on Curcumae Rhizoma was the study on the variety C. phaeocaulis, but somehow nowadays there are few studies on it. Actually, most studies on the C. longa focus on the rhizome. But according to one paper [25], it indicated clearly that the rhizome and radix of C. longa were traditionally used as two Chinese medicines, respectively, named Jianghuang and Yujin in Chinese.

Toxicity
Although Curcumae Rhizoma and Curcumae Radix have long been used in medicine clinics, systematic safety and toxicity evaluations are rare until now. Several experiments were conducted to explore the acute toxicity and genetic toxicity of Curcumae Rhizoma but found to be nontoxic [58,60]. The extract curcumol as a principle component of Curcumae Rhizoma is proved to be no obvious lethal effect through the long-term toxicity experiment. Different dosage groups at 1.16 g/20 mL, 2.32 g/20 mL, and 4.64 g/20 mL Tween 80, respectively, were set up, and the effect of the extracts to the rat's kidney was found to be small at low and middle but have a certain toxicity at high dose. Generally speaking, it could be used safely at low and middle dosage [124]. It is reported that Curcumae Rhizoma is harmful to reproductive system to a certain extent. It could decrease the rate of pregnant of female mice, increase the rate of teratogenicity, excite uterine smooth muscle, and reduce the weight of mice's testis and seminal vesicle. Curcumae Rhizoma is pregnancy-contraindicated drug which should be used with caution [125,126].
The LD 50 of mice was found to be different in sources of Curcumae Radix. C. wenyujin is the most serious [127], in which LD 50 is 80.98 g/kg. It is equal to 485.9 times the dose of the maximum clinical dosage of adults, which demonstrated that it is safe in clinical medicine usage [128].

Quality Control
As described previously, the four species scatter across the south and southwest of China, determined to the various differences among every producing area. So the variety of them makes it difficult for us to control its quality. In the Pharmacopoeia of People's Republic of China, there are two marker components, the essential oil [no less than 1.5% (mL/g)] and germacrone (as control), chosen to control the quality of Curcuma Rhizoma. When it comes to Curcuma Radix, its standard crude drug is regarded as examine item. And thin layer chromatography (TLC) was used to get the result for the two medicines [129]. However, due to the complex constituents and multifarious pharmacological activities, the test methods mentioned above might be insufficient and inconvenient to fully illustrate the quality of them. Nowadays, to evaluate the quality of the two medicines more accurately, many ways of qualitative identification and determination of content are developed, such as twice development TLC [129], high performance liquid chromatography (HPLC) [130], fingerprinting [131], GC-MS, and pressurized liquid extraction (PLE) [132]. Among these, fingerprinting is the most widely applied. Also it is a tendency that multimethods using jointly are more rapid and reliable, such as UPLC/QTOF/MS method [133], HPLC-UV-MS [134], GC-MS fingerprinting, and HPLC fingerprinting [135]. Generally speaking, for Curcuma Rhizoma, curcumin is regarded as one of the effective constituents, germacrone chosen as the characteristic component, while the essential oil is the effective part. These three marker items have significant differences from variety to variety of Curcuma Rhizoma.
As for Curcuma Radix, the marker components are the same with Curcuma Rhizoma; otherwise, curdione may also be recommend to be the standard of its quality control [136]. We are still exploring the better ways to make the quality control of Curcuma Rhizoma and Curcuma Radix more rapid and reliable. And we should also extend the studying filed of quality control, not only limited to the present methods and active components.

Concluding Remarks
In addition to the four official species, C. wenyujin, C. longa. C. kwangsiensis, and C. phaeocaulis, there are also several other varieties which are misled as Curcumae Rhizoma and Curcumae Radix. They are C. Zedoaria, C. Aeruginosa, C. Aromatic, and C. xanthorrhiza [115,[134][135][136][137] which are also been used as Curcumae Rhizoma or Curcumae Radix or other medicines. But it is not recoreded in the Chinese Pharmacopoeia. Curcumae Rhizoma and Curcumae Radix, two wellknown and widely used Chinese medicines of the family Zingiberaceae, have been traditionally used for thousands of years. Although they both have effect in invigorating the circulation of blood, Curcumae Rhizoma and Curcumae Radix have their own respective features and priorities. However, the studies of them are disproportionate on variety and source. The traditional uses have been increasingly confirmed by hundreds of pharmacological studies in vitro and in vivo. Particularly, the antitumor studies of these two medicines are becoming more and more popular. Compared to Curcumae Rhizoma, the range of Curcumae Radix applying on this aspect is, respectively, small which only limits the treatment of gastric cancer. In most cases, there is no difference between the two medicines. Although studies on the pharmacological effects and their mechanisms have been performed, yet further study is still urgently needed to gain a better understanding of the differences between the two medicines in order to provide better service for clinical care.
Besides, most of the extracted chemical compositions have been found in the rhizoma of C. wenyujin and C. longa. So we should devote ourselves more into the chemical constituents' study of other species and focus more on Curcumae Radix.
In addition, recently, fingerprinting has been the most popular way to describe the chemical constituents and to control the quality of Curcumae Rhizoma and Curcumae Radix. But we still need to explore better ways to make the quality control more rapid and reliable.
Single herbs usually exert their efficacy in the form of prescriptions in TCM clinic. But the research of Chinese herbal prescriptions is someway complex, which makes it difficult to draw accurate conclusions and reflects the role of the single herb. And the study of single herb is difficult to reflect its true role in the TCM prescriptions. So we suggest that we could study Curcumae Rhizoma and Curcumae Radix in couplet medicines, such as Curcumae Radix and Sparganii Rhizoma, Curcumae Rhizoma and Sparganii Rhizoma which is similar in effect or clinical application and so on. But during study, we still should not ignore how the couplet medicines play an important part in the Chinese herbal compound prescriptions.
At last, through reading plenty of documents, we have found a problem. In recent years, many new reports have reported a large number of new compounds, but the relevant reports of their pharmacological activities are very less. And we still could not find a quantitative evaluation criterion which is exclusive and accurate enough to quality control these two medicines. So we should study further the discovery and identification of new components and medicinal efficacious composition, pharmaceutical activity screening, and evaluation and find more model of pharmacology of these two medicines. In a word, more experiments including in vitro, in vivo, and clinical studies should be encouraged to be put into effect to make us understand why Curcumae Rhizoma and Curcumae Radix are effective in more detail so as to make contribution to Chinese medical career.