Phytochemicals and Medicinal Properties of Indigenous Tropical Fruits with Potential for Commercial Development

Hundreds of fruit-bearing trees are native to Southeast Asia, but many of them are considered as indigenous or underutilized. These species can be categorized as indigenous tropical fruits with potential for commercial development and those possible for commercial development. Many of these fruits are considered as underutilized unless the commercialization is being realized despite the fact that they have the developmental potential. This review discusses seven indigenous tropical fruits from 15 species that have been identified, in which their fruits are having potential for commercial development. As they are not as popular as the commercially available fruits, limited information is found. This paper is the first initiative to provide information on the phytochemicals and potential medicinal uses of these fruits. Phytochemicals detected in these fruits are mainly the phenolic compounds, carotenoids, and other terpenoids. Most of these phytochemicals are potent antioxidants and have corresponded to the free radical scavenging activities and other biological activities of the fruits. The scientific research that covered a broad range of in vitro to in vivo studies on the medicinal potentials of these fruits is also discussed in detail. The current review is an update for researchers to have a better understanding of the species, which simultaneously can provide awareness to enhance their commercial value and promote their utilization for better biodiversity conservation.


Introduction
Southeast Asian countries, including Malaysia, have tropical rainforests with a variety of fruit-bearing trees. These trees are evergreen and growing throughout the year. Many of these trees produce edible fruit for animals living within the scrubs and some of these fruits are even used by the local communities in their traditional medicine [1]. Since centuries ago, human started to cultivate different plant species to harvest their edible fruits as food sources. In the ancient days, cultivation of the fruit-bearing trees for their edible fruits was done only by planting them beside the house or around the housing areas. Hence, the fruits can be easily harvested during the fruiting season. Large-scale farming has been introduced and started in the later years for commercialization of these tropical fruits due to increasing in their market demand.
Today, many of the tropical fruits have been commercialized.   Evidence-Based Complementary and Alternative Medicine apple is milky in color. Some varieties of S. malaccense plant have red colored fruit. The fruit of Z. mauritiana is native to Indonesia, India, and China. In Malaysia, the fruit of Z. mauritiana is commonly used in culinary practices.

Indigenous Tropical Fruits That Are Possible for Commercial Development
According to the Department of Agriculture Malaysia, over 370 species of fruit-bearing trees are found in Malaysia [50]. Even though most of these trees are wildly grown, some of them bear fruits with commercial values. In this review, eight indigenous fruits from different genera categorized as fruits that possible for commercial development in Malaysia or Southeast Asia have been discussed. The fruits of Averrhoa bilimbi, Baccaurea macrocarpa, Baccaurea motleyana, Cynometra cauliflora, Durio kutejensis, Garcinia hombroniana, G. parvifolia, and Phyllanthus emblica are categorized in this group. Their common names are tabulated in Table 1.
All of these fruits are belonging to different plant families, except Baccaurea and Phyllanthus fruits, which belong to the Phyllanthaceae family. Out of 2000 species, only certain plant species from Phyllanthaceae are cultivated in the tropical countries. Phyllanthus emblica (also known as Emblica officinalis) is locally known as "Pokok Melaka"; it is another underutilized plant native to Malaysia. The name of Malacca (Melaka) state, a historical city in Malaysia, is originated from the P. emblica trees that are well grown along the riverside. Its fruit is not popular among Malaysians and hence it is only homegrown in some areas of Malaysia. Although P. emblica trees have been planted for the ornamental purpose, the fruit has been reported as a potential source of functional food because it contains a high amount of vitamin C [51]. In India, the fruit of P. emblica is traditionally eaten by steeping the sour fruit in turmeric and adding it to salt water to make it palatable [52]. The extract of P. emblica fruit has also been used as hair dye [53]. Other fruits of the family Phyllanthaceae are Baccaurea fruits, which include B. macrocarpa (tampoi) and B. motleyana (rambai). These species are widely cultivated on the west coast of Peninsular Malaysia, especially in Perak, a state in Malaysia. Due to the annual fruiting season [54], Baccaurea fruits can be found only in the local markets during the months of peak fruiting period. The trees of B. motleyana are also found in other parts of Southeast Asia, especially Thailand, mainly for fruit cultivation. "Rambai" is the Malay name while "mafai-farang" is the Thai name of B. motleyana fruit (Table 1).
A. bilimbi, also known as "belimbing buluh" or cucumber tree, is native to Malaysia and Indonesia. It has been cultivated in Southeast Asia. In India, the trees of A. bilimbi are planted in the home gardens. A. bilimbi fruit is lesser popular for consumption than the commercially known star fruit (A. carambola). However, it is traditionally used as medicine for curing several diseases, including cardiovascular diseases [55]. Besides vitamins and minerals, the fruit of A. bilimbi also contains flavonoids and triterpenoids that contribute to its beneficial health properties [56]. Besides A. bilimbi, C. cauliflora is another homegrown fruit-bearing tree that is found primarily in rural areas of Peninsular Malaysia. Its fruit is locally called as "nam-nam." The fruit of C. cauliflora has savory taste and can be consumed as fruit salad.
G. hombroniana is another plant native to Malaysia. The tree bears fruit called "seashore mangosteen" [27]. Another species of Garcinia, G. parvifolia, is also one of the indigenous tropical plants [57]. Garcinia fruits contain xanthones, flavones, and triterpenoids as the bioactive phytochemicals besides the leaves and twig of Garcinia trees [58]. D. kutejensis is another type of durian plant. The color of its flesh is orange-reddish due to the high amount of carotenoids. It is native to Borneo region, called as durian nyekak in East Malaysia. It has the taste and texture similar to the fruit of common D. zibethinus. The fruits of D. zibethinus or commercial durians are the most famous fruits in Malaysia and Thailand. However, the fruit of D. kutejensis is not available in Peninsular Malaysia owing to the fact that the trees of this fruit are native to Java and Borneo Islands [59]. D. kutejensis is a wildly grown species, and its fruits are collected by the indigenous people of Borneo Island (including Sabah and Sarawak) for their own consumption or selling in the local market. Therefore, D. kutejensis fruit is considered underutilized in Malaysia. Hence, in Peninsular Malaysia, the fruit of D. kutejensis cannot be found in the local markets throughout the year.

Industrial Applications of Indigenous Tropical Fruits and Their Potential as Commercial Products
In Southeast Asia, actually many indigenous tropical fruits have potential to be commercialized. Dabai is an indigenous tropical fruit that is almost similar to olive. The oil extracted from the pulp of dabai demonstrated some possible health benefits [60]. In Sarawak (East Malaysia), the edible part of dabai has been incorporated into local cuisines such as fried rice, omelet, and being developed into the form of sauce or paste as an ingredient for cooking. Bambangan, as one of the big mangoes in the world [61], has also been used in cooking and as dessert. Bambangan juice is commonly consumed by the local people of Sabah (East Malaysia). In Sabah and Kalimantan, bambangan pickled can be seen being sold in the local markets, whereas bambangan peel is used as a raw ingredient for some local dishes. Besides dabai and bambangan, bananas (Musa spp.) from Borneo region are processed into banana chips.
On the other hand, in Peninsular Malaysia, bacang (M. foetida), kundang (B. macrophylla), and jambu (Syzygium  spp.) are those fruits that are having the potential for development into commercial products, such as canned fruit, pickles, and fruit juices. Although bidara (Z. mauritiana) is one of the commercialized fruits in India [70], this fruit is not commonly consumed by Malaysian. The fruit is only freshly eaten or preserved as pickle by Malay community.

Phenolic Compounds.
Phenolic compounds are the largest group of phytochemicals and are widely distributed throughout the plant kingdom. Phenols, as the major bioactive substances in fruits, play a vital role as antioxidant. The major phenolic compounds in plants are shown in Figure 1. Phenolic compounds are good antioxidants found in the flesh of fruits including phenolic acids and flavonoids, whereas flavonoids and lignans are found in the seeds or kernel [71]. Among the phenolic acids, gallic acid is the major component of plant. Each fruit has, at least, a few major phenolic compounds. In addition to fruit, catechin is one of the main flavonoids found in leaves. Since phenolics are potent antioxidants, increased consumption of a mixture of fruits daily should be able to provide an adequate phenolic antioxidant. Thus, proper knowledge concerning identity and amount of phenolics in indigenous tropical fruits helps to promote the usage of these underutilized tropical fruits for their functional benefits. Total phenolic content (TPC) is one of the most popular indicators for estimation of phenolic antioxidants in fruit. Determination of TPC is straightforward and easy to perform using Folin-Ciocalteu reagent and usually expressed as gallic acid equivalent (GAE) ( Table 2). Based on previous literature, B. macrophylla fruits have not been determined for TPC. Table 2 also depicts the phenolic compounds identified and quantified in selected indigenous tropical fruits. Among the indigenous tropical fruits with potential for commercial development, S. malaccense fruit has the least TPC, whereas the other fruits have moderate to high TPC. TPCs of M. foetida fruit extracts ranged from 122.8 to 199.8 mg GAE/100 g of edible portion (EP) [10]. However, a wide range of total phenolics determined in the same type of fruit could be due to the different methods used, as well as the fruit variety and geographical distribution [12].
Among the indigenous tropical fruits, flavonoids are the major antioxidants found in these fruits. As shown in Table 2, a few flavonoids have been identified in C. odontophyllum fruit (dabai), and some unknown flavonoids were detected in dabai pulp [24]. Due to the dark purple color of dabai peel, anthocyanins should be the major phenolics in its peel. Chew et al. [23] have reported different types of anthocyanins that were detected in the dabai peel, such as cyanidin glucoside, malvidin glucoside, and peonidin glucoside. Anthocyanins were also found in the fruits of S. malaccense and A. bilimbi. Reynertson et al. [43] reported as much as 0.02 g/g of cyanidin-3-glucoside that was determined in the peel of dried S. malaccense fruit. The peel might also contain carotenoids and betacyanins because it is red in color. Moreover, a nonpurple colored A. bilimbi fruit exhibited a high concentration of total anthocyanins (47.36 mg/100 g fresh weight) [18]. However, total anthocyanin content (TAC) determined in the purple colored extract of defatted C. odontophyllum fruit peel was less than 40 mg/100 g dry weight (DW) [24]. The nonpurple colored extract of A. bilimbi could have a low TAC because anthocyanins are red-purplish color pigments. The difference could have been due to the use of colorimetric method through pH differential that resulted in an overestimation of TAC.

Mangifera foetida
To date, only a very limited information on phenolic compounds is available for the scientific community, especially phenolic compounds in the fruits of Baccaurea, Cynometra, and Garcinia. Besides that, volkensiflavone is one of the potential flavonoids in G. hombroniana fruit [28], and garcinidon A has been discovered in the peel of G. parvifolia [73]. D. kutejensis fruit also contained 0.03 g of tannin in one gram of dried fruit [25].

Carotenoids.
Among the plant phytochemicals, carotenoids are classified as terpenoids. The compounds are found abundantly in yellow to orange-and orange to red-colored fruits. Carotenoids are grouped into carotenes and xanthophylls. In nature, -carotene is the most abundant type of carotene, while lycopene is the primary phytochemical in orange-red colored fruits. Among the xanthophylls, lutein is typically detected in green leafy vegetables. However, some fruits also contain lutein [4]. Figure 2 shows the major types of carotenoid in fruit.
Among the carotenes, all-trans -carotene is the most common type of carotenoid found in plant because it is part of the antioxidant defense system at cellular level of a plant. Some green-colored fruits may contain a high amount of carotenoid because the yellow-orange-colored carotenoid pigments are masked by chlorophylls [88]. The intake of carotenoids from various plant sources is thought to be able to maintain good health. In this review, different carotenoids and their concentrations in the selected indigenous tropical fruits are shown in Table 3.

Garcinia hombroniana
No report from the literature No report from the literature

Evidence-Based Complementary and Alternative Medicine
Among the fruits, yellow to orange-colored fruits have high -carotene contents, whereas lycopene is the orangered color pigment. Carotenoids contents in some commercialized fruits and vegetables have been reported by Khoo et al. [4]. However, carotenoid contents in other indigenous tropical fruits remain unknown. Many of the indigenous fruits possible for commercial development do not contain any carotenoid. Whitish-colored fruits have little or trace amount of carotenoids, especially the endocarp. No study has been performed to determine the carotenoid contents of B. motleyana, C. cauliflora, G. hombroniana, and Z. mauritiana fruits. It is possibly due to the low concentrations of carotenoid in these fruits.
As previously reported, the fruit of B. macrocarpa (tampoi) contains carotenoids. However, -carotene (a major carotenoid) was not detected in tampoi [53]. There is a broad range of total carotenoid contents found in some of the indigenous tropical fruits (0.003-29 mg/100 g DW) ( Table 3). For example, the different varieties of pumpkin have total carotenoids ranging between 0.06 and 14.9 mg -carotene per 100 g fresh weight [4].
Based on the previous study, the total carotenoid content (TCC) of horse mango (M. foetida) was ranged from 96.5 to 153.0 g -carotene equivalent (BCE)/100 g EP [10]. In durian nyekak (D. kutejensis), the TCC was 11.16-14.97 mg BCE/100 g DW [54]. Although some of the indigenous tropical fruits have a moderate level of TCC (Table 3), the cucumber tree (A. bilimbi) was found to have a highercarotene content (28.99 mg/100 g dry weight) than the other indigenous tropical fruits [59]. Besides that, P. emblica only has 0.01 mg of -carotene in the fruit pulp (per 100 g edible pulp) [50].

Terpene and Terpenoids.
Monoterpenes, diterpenes, triterpenes, and sesquiterpenes are some of the terpenes discussed in this review. Terpenoid is a vast and diverse class of natural occurring organic chemicals related to terpene [89]. Most of the terpenoids including saponins are possible antioxidants [90]. Besides antioxidant activity, saponins have several health benefits [91]. Among the terpenes and terpenoids, some are volatile compounds found in plants. Geraniol, limonene, linalool, and pinene are some of the volatile components detected in fruit samples ( Figure 3). Terpenes, mainly sesquiterpenes, have been identified in the root, bark, flowers, and leaves of plants [92]. Only a few terpenes have been discovered in fruits. Although many studies have been performed on volatile terpenes in essential oils of plants, most of the studies analyzed the other parts of the plant rather than the fruit. From our literature search, a minimum of 20 volatile components including terpenes were found in different parts of the plant. Little information on terpenes and terpenoids content in fruit is available for the scientific community, especially the underutilized and indigenous tropical fruits.
It can be observed in Table 4 that some indigenous tropical fruits with potential for commercial development are well studied for terpenes and terpenoids contents, but not for the fruit of Z. mauritiana. Umaru et al. reported that Indian   (Table 4). Terpenes and terpenoids are natural phytochemicals identified in plants. Fruit contains some terpenes, such as monoterpene, triterpene, and sesquiterpene. For the indigenous tropical fruits with potential for commercial development, such as B. macrophylla, M. foetida, M. pajang, S. jambos, and S. malaccense, some terpenes and terpenoids have been identified in the extracted essential oil of these fruits (Table 4). Besides carotenoids, saponin is one of the terpenoids found in the defatted dabai [24]. Among the indigenous tropical fruits possible for commercial development, B. motleyana and P. emblica fruits have low concentrations of terpenes, terpenoids, and saponins. Wong et al. reported that terpenes are the minor components in the essential oil of rambai (B. motleyana) [74]. Saponin is one of the members of the triterpenoid group [93]. It has been discovered in Indian gooseberry (P. emblica) [37]. In Cuba, a study has identified -pinene, p-cymene (0.02), limonene, 1,8-cineole, -terpinene, terpinolene, -terpineol, -cadinene, -calacorene, and other volatile components in the essential oil of A. bilimbi fruit [19]. These compounds are monoterpenes and sesquiterpenes commonly found in plants. The essential oil of P. emblica fruit containscaryophyllene and -bourbonene as the major terpenes [80]. Besides that, the fruit of G. hombroniana has two novel triterpenes (17,14-friedolanostanes and lanostanes) [76]. Terpenoids, such as saponins, are the important phytochemical constituents in combating the infectious diseases and terpenoids are primarily discovered as the potent antimicrobial  Baccaurea macrocarpa No report from the literature No report from the literature

Ziziphus mauritiana
No report from the literature Saponin: 7.13% [49] 12 Evidence-Based Complementary and Alternative Medicine Syzygium jambos [86] Freshly eaten, made into jam and served as dessert (whole ripe fruit) Ripe fruit is used as a tonic for brain and liver and as a diuretic; seeds for treatment of diarrhea, dysentery, and catarrh 14 Syzygium malaccense [86] Freshly eaten (whole ripe fruit), as pickle and used in cooking dishes (unripe fruit) Fruit decoction as a febrifuge 15 Ziziphus mauritiana [86,87] Freshly eaten as salad or pickle, and used in cooking dishes (whole ripe fruit) Ripen fruit for treatment of sore throat and cough; seed for treatment of diarrhea and weakness of stomach agents. Antimicrobial effects of the essential oils of many fruits have been reported by Nychas [94].

Medicinal Properties of 15 Indigenous Tropical Fruits
Fruits are commonly consumed for their nutrients, and some fruits are used as medicine. The medicinal properties of fruits are closely related to their available phytochemicals, as well as antioxidants. Many of the indigenous fruits have been traditionally used as folk medicine. These fruits contain phytochemical antioxidants that can prevent, treat, and cure various types of diseases. Many phytochemicals such as carotenoids, tannic acids, triterpenes, and some flavonoids are free radical scavengers that can contribute to the suppression of oxidative stress and anti-inflammatory effect in the human body [95]. The details on the applications of 15 selected indigenous fruits as food and as folk medicine are tabulated in Table 5. Additionally, the medicinal values of these indigenous tropical fruits reported by previous scientific reports are listed in Table 6. Among the 15 indigenous tropical fruits, the flesh of five fruits are not scientifically determined for their medicinal values, except for antioxidant activities. The other fruits have been studied for antimicrobial effects (including fungal) and several protective effects against chronic diseases. Among the scientific evidence shown in previous literature, most of the experiments are mainly focused on in vitro and animal models. Limited studies on human intervention trials allow researchers or scientists to study the potential health effects of these underutilized tropical fruits using human models in the future. In this review, the medicinal properties of the selected underutilized tropical fruits are discussed. The protective effects of these fruits against several diseases are shown, either as folk medicines or with scientific evidence. Overall, among the 15 indigenous tropical fruits, the fruits of Evidence-Based Complementary and Alternative Medicine 13

Baccaurea macrocarpa
No report from the literature

Bouea macrophylla
No report from the literature

Garcinia hombroniana
Phenolic compounds Inhibition of platelet aggregation and LDL-peroxidation [26] Human whole blood from healthy subjects: in vitro LDL oxidation and antiplatelet aggregation assay.

Mangifera foetida
No report from the literature

Phenolic compounds
Gastric ulcer healing effect [118] Indomethacin-induced ulceration of rats Anticlastogenicity [119] Cochran-Armitage trend test: bone marrow cells of Swiss albino mice treated with lead and aluminum Antiproliferative [120] MTT assay: MCF-7 tumor cell culture Antimicrobial [41] TLC-bioautographic method: drug-resistant bacteria and yeast Anticancer [121] In vitro cytotoxicity assays: human lung carcinoma (A549) and HepG2 cell lines Antiaging effect [122] In vitro MMP-1, MMP-2, and elastase inhibition assays: inhibitions of collagenase and elastase Chondroprotection [123] In vitro enzymatic assays: explant cultures of cartilage from osteoarthritis patients Syzygium jambos Phenols, tannins, alkaloids, and flavonoids Antifungal (seed) [ (Table 5). However, three out of these 15 indigenous tropical fruits have not been scientifically determined for their medicinal properties and potential health benefits. These fruits are B. macrocarpa, B. macrophylla, and M. foetida (Table 6). Among hundreds of fruit species, the fruit of A. bilimbi (cucumber tree) is one of the potential sources of antioxidant that offers health benefits. According to Ambili et al., the extracts of A. bilimbi exhibited the cholesterol-lowering potential in rats [96]. The water extract of A. bilimbi fruit (0.8 mg/kg body weight, BW) also improved lipid profile in Triton-induced hypercholesterolemia in rats [96]. Other than that, the active fraction of the water extract at a dose of 0.3 mg/kg BW possessed an optimum antihypercholesterolemic activity. The fruit (125 mg/kg BW) and its water extract (50 mg/kg BW) also effectively improved the lipid profile of the rats fed with high-fat diet.
Another study reported that the fruit of A. bilimbi has antidiabetic effect studied using streptozotocin-induced diabetic rats [97]. The flavonoids, carotenoids, and terpenes could be the potent bioactive compounds in A. bilimbi fruits that provide the antidiabetic effect. Besides that, this fruit is also reported as an active antimicrobial agent. Chloroform and methanolic extracts of this fruit (bilimbi) were reported to have good inhibitory activities on several types of bacteria, such as Aeromonas hydrophila, Escherichia coli, Klebsiella pneumoniae, Saccharomyces cerevisiae, Staphylococcus aureus, Streptococcus agalactiae, and Bacillus subtilis [98]. Hence, this fruit has been used in folk medicine for easing whooping cough [85]. The scientific evidence for the role of phytochemicals in A. bilimbi fruit extract as health-promoting agents is inadequate. Most of the studies focused only on in vitro and animal models. Up to date, there is no human-based scientific evidence to support its use in the prevention of such diseases.
Flavonoids and anthocyanins in dabai fruit (C. odontophyllum) are the potent antioxidants. The defatted dabai extract (5%) was shown to significantly reduce the levels of total cholesterol and low-density lipoprotein-cholesterol in rabbits supplemented with high-cholesterol diet for eight weeks as compared to the control group [99]. Besides that, rabbits fed a high-cholesterol diet and defatted dabai pulp have a significant increment in high-density lipoprotein level [100]. The severity of atherosclerotic plaques in the high-cholesterol diet rabbit group that supplemented with defatted dabai extracts was also reduced compared to the control group. Therefore, the fruit extract of defatted dabai can be considered as a new source of nutraceutical due to its antiatherosclerotic properties. However, no human-based study has been performed to prove the cholesterol-lowering effect of the defatted dabai extract. Human intervention trial is recommended for future study to test the efficacy of defatted dabai parts because dabai is one of the underutilized fruits highly potent to be commercialized.
There are other medicinal uses which were found on D. kutejensis, but it may possess some anti-inflammatory properties as it has many similarities to the D. zibethinus, where the methanolic extracts of D. zibethinus fruit were reported to have anti-inflammatory effects [101]. The extract of B. motleyana peel possessed antimicrobial activities since it inhibited the growth of S. aureus, B. cereus, B. subtilis, E. coli, Pseudomonas aeruginosa, and Proteus vulgaris [102]. The fruit of Cynometra cauliflora possesses antiproliferative activity by inhibition of cytotoxic effect to human promyelocytic leukemia HL-60 cells [103].
Generally, most of the plants from genus Garcinia have medicinal effects [104]. In Southeast Asia, only a few studies were reported on the potential medicinal properties of underutilized Garcinia fruits. The fruit extract of G. hombroniana inhibited in vitro lipid peroxidation and had antiplatelet activities [26]. Other than the fruits, Kapadia and Rao also report antimicrobial effects of Garcinia plants towards bacteria, fungus, and other parasites [57]. The stems and leaves of three Garcinia plants indicate platelet-activating factor antagonist activity [105]. Among the three Garcinia plants, the leaves of G. hombroniana (seashore mangosteen) have higher microbial inhibition activity (46.3%) than the leaves and stems of G. cowa (cowa) and G. dulcis (mundu). The main bioactive compound in the leaves that possess this antimicrobial effect is reported as garcihombronane [57].
Mangifera fruit, also known as mango, is traditionally used for its medicinal properties. The kernel of M. pajang (Borneo mango) has an anticancer effect [106], and the fruit extract was found to possess hepatoprotective effects [107]. Ibrahim also reported the antiatherosclerotic and antihypercholesterolemic effects of fruit juice powder of M. pajang tested using New Zealand white rabbits [36]. Then, a human clinical trial was carried out to verify the efficacy of M. pajang fruit juice which also demonstrated a promising effect. Healthy subjects supplemented with M. pajang fruit juice showed better blood lipid parameters compared to the placebo group [108]. The antihypercholesterolemic effect of M. pajang fruit juice could be due to the antioxidative effect of polyphenolics, vitamin C, and -carotene in the juice. A 12 weeks, double-blind, placebo-controlled clinical trial also confirmed that antioxidants (24 mg -carotene B, 1000 mg vitamin C, 800 IU vitamin E) supplementation significantly increased the plasma high-density lipoproteincholesterol in 45 coronary artery disease patients [109]. Both studies have proven that antioxidant supplementation helped in improving plasma lipid profile.
In addition to M. pajang fruit, M. foetida fruit pulp (without peel) also demonstrated antioxidant activity [30]. On the other hand, the leaf extracts of M. foetida have an antimicrobial activity for S. aureus, but not for E. coli, S. cerevisiae, and Fusarium oxysporum [110]. Besides these findings, other medicinal effect has not been determined for M. foetida (horse mango) fruit, except for its antioxidants in the inhibition of oxidative stress [111].
Emblic (P. emblica) fruit, also called as Indian gooseberry, is traditionally known for its medicinal value for treating cough and asthma [85]. The fruit is traditionally used in India for the treatment of several health complications, such as diarrhea, dysentery, anemia, jaundice, and cough [112]. The fruit is also rich in antioxidant. Liu et al. reported that phenolic compounds extracted from emblic fruits were highly correlated with their antioxidant activities [40]. Various parts of P. emblica plant have also been used as Indian Ayurvedic medicine. Besides that, phytochemicals in the plant parts are well known for their medicinal values, such as antidiabetic, antibacterial, antiulcerogenic, antiproliferative, and hypolipidemic effects [113].
A study on the healing activity of ethanolic extract of emblic fruit (P. emblica) has shown some positive results, where the rats were induced with indomethacin (30 mg/kg BW, oral intubation) [118]. The results showed that the extract (100 mg/kg BW) of this fruit had significantly reduced the lipid peroxidation parameters (MDA, carbonyl, total DNA, SOD, and CAT), ulcer index (3.8), and DNA damage induced by indomethacin (85.73% of protection) in rats after seven days of postulcerative treatment compared with the controls. Other than that, the extract of emblic fruit also inhibited the growth of Staphylococcus aureus, Bacillus subtilis, Salmonella paratyphi, Shigella dysenteriae, and Candida albicans, although no inhibition of Escherichia coli growth was observed [41]. Also, the aqueous extract of emblic has shown the potential as an anticancer agent, where the extract inhibited the growth of human lung carcinoma and (A549) and human hepatocellular carcinoma (HepG2) cell lines [118]. Moreover, the emblic fruit powder demonstrated a significant chondroprotective effect based on an in vitro model of cartilage degradation in explant cultures of articular knee cartilages obtained from osteoarthritis patients [123].
Limited information on medicinal properties of selected Syzygium fruits (S. jambos and S. malaccense) is available. The fruit of S. jambos (rose apple) has been traditionally used as an astringent and for brain and liver, as well as digestive problems [127]. Other than the use of Syzygium fruits as folk medicine, scientific research reported that the aqueous fruit extracts of S. jambos reduced the in vitro -glucosidase and -amylase inhibitory activities [128]. Other than these two Syzygium fruits, the fruit extracts of S. samarangense (samarang apple) were also as effective as antibiotics to inhibit microbial activities [129]. The fruit extract of S. cumini (Java plum) is also a potential antidiabetic agent [130].
Most of the literature only reported on the medical properties of different aerial parts (mainly leaves and bark) of underutilized plants instead of their fruits [131]. For example, the leaves of many plant species have antimicrobial activities. The methanolic extracts of S. jambos leaves were tested for antimicrobial activity, where the extracts inhibited the growth of some Gram-positive and Gram-negative bacteria [132]. Besides that, antimicrobial activities of the extracts of bark, leaves, and seeds of S. jambos have also been reported by Murugan et al. [133]. The leaves of S. malaccense (Malay apple) were reported to be useful for preventing inflammation [134]. Moreover, the extracts of different parts of Syzygium trees that have antidiabetic properties were documented in a review article [135].
Z. mauritiana (Indian jujube) is another fruit that is not well studied for its medicinal properties. The only therapeutic properties of the fruit are only available as reported in their traditional uses for treating abscesses, wounds, anodyne, and tonic, as well as styptic and purifying blood [87]. Until now, no human intervention study has been performed to determine the wound healing effect of Z. mauritiana fruit or its fruit extract. However, the leaves of Z. mauritiana were reported to significantly prevent leucopenia and noiseinduced enhancement of neutrophil function in Guinea pigs compared with diazepam, in which the Guinea pigs were subjected to 100 Db industrial noise (8-50 kHz) [136]. Antioxidant activities have also been determined for the fruits from two varieties of Z. mauritiana, and the IC 50 values of the ethanolic extract of both varieties (Beri and Narikeli) were 72 and 250 g/mL, respectively [137]. The seed of Z. mauritiana has also been studied for its anticancer and antidiabetic potentials. The ethanolic extracts of Z. mauritiana seed were found to induce cancer cells death and significantly reduced tumor volume and tumor cell count in albino mice after 13 days of treatment with the extract (100-800 mg/kg BW) [138]. Besides that, the seed extract exhibited hypoglycemic activity, where administration of the extract (at a concentration of 800 mg/kg BW) reduced weight loss and mortality of alloxan-induced diabetic mice [139]. Alternatively, the root of Z. mauritiana has been traditionally used to treat ringworm by applying the root paste [140] and inhibition of microbial activities, such as Bacillus subtilis, Staphylococcus aureus, and Mycobacterium phlei [141].
On the contrary, some human intervention trials did not support the beneficial effects of antioxidant supplementation [142]. Although there was a dose-dependent relationship between antioxidative activity and antioxidant compound [143], an overdose of a particular bioactive compound may have prooxidative effect in the human body. Therefore, a moderate amount of antioxidant supplementation is suggested. Owing to lack of human-based scientific evidence, it is suggested that human intervention trials should be conducted in future studies to shed more light on the efficacy of potential bioactive components derived from these underutilized tropical fruits in any disease prevention. Although a part of these fruits have been studied for their medicinal properties, substantial scientific data is still lacking and the researches are still at a very preliminary stage. Future studies need to be performed for the fruits of B. macrocarpa, B. macrophylla, D. kutejensis, M. foetida, and S. jambos as there is no available data on these fruits until they are studied.

Conclusions
Southeast Asia, including Malaysia, consists of countries rich in plant biodiversity that possess more than a thousand types of fruit-bearing trees. Some of these fruits are already commercialized, but many are remaining underutilized. Nowadays, some of these trees are at least cultivated by the villagers or local farmers in the traditionally way for their fruits. Hence, identification of those indigenous tropical fruits with potential for commercial development can help researchers, farmers, or industry to see the opportunities from these native fruits. Indigenous tropical fruits are rich in phytochemicals, especially phenolic compounds, carotenoids, terpenes, and other terpenoids. Instead of providing the attractive colors of the fruits, phytochemicals also offer protective effects against chronic diseases, such 16 Evidence-Based Complementary and Alternative Medicine as cardiovascular diseases, diabetes, and cancers. They are also responsible for the anti-inflammatory and antimicrobial effects, as well as other medicinal values of the fruits.
Scientifically, extra efforts are needed for studies emphasized on the beneficial health properties and toxicity effect of the fruit using animal-based experiments as well as human interventions to strengthen the scientific proof of their beneficial health properties. Studies on the toxicity effects of the fruits or their extracts should not also be neglected. Due to the variation in health benefits and bioactive phytochemicals in these fruits, attention should be given to study the efficacy of these fruits in combating diseases and later turning them into nutraceutical or basic ingredients for functional food. Bioactive compounds isolated from these fruits could also be used as nutraceutical and pharmaceutical ingredients. Primary screening of antioxidant properties and medicinal values for those indigenous tropical fruits without any scientific evidence is recommended to provide basic understanding for advance research. All the information is useful for the authorities concerned to promote the consumption of these fruits all around the world.