Structural and Functional Analysis of the Complex between Citrate and the Zinc Peptidase Carboxypeptidase A

A high-resolution carboxypeptidase-Zn2+-citrate complex was studied by X-ray diffraction and enzyme kinetics for the first time. The citrate molecule acts as a competitive inhibitor of this benchmark zinc-dependent peptidase, chelating the catalytic zinc ion in the active site of the enzyme and inducing a conformational change such that carboxypeptidase adopts the conformation expected to occur by substrate binding. Citrate adopts an extended conformation with half of the molecule facing the zinc ion, while the other half is docked in the S1′ hydrophobic specificity pocket of the enzyme, in contrast with the binding mode expected for a substrate like phenylalanine or a peptidomimetic inhibitor like benzylsuccinic acid. Combined structural and enzymatic analysis describes the characteristics of the binding of this ligand that, acting against physiologically relevant zinc-dependent proteases, may serve as a general model in the design of new drug-protecting molecules for the oral delivery of drugs of peptide origin.


Introduction
Carboxypeptidase A (CPA) is the canonical form of the M14 family of peptidases, an important group of physiologically relevant enzymes [1]. M14 peptidases such as CPA and CPB, synthesized in the pancreas and secreted into the gastrointestinal tract, are the major zinc-dependent enzymes responsible for the release of the C-terminal amino acid residue from food protein [2]. Carboxypeptidase activity in the gastrointestinal tract may be modulated by dietary zinc [3,4] or can be inhibited by some organic food components [5][6][7]. Also, changes in the genomic expression levels may control carboxypeptidase enzyme activity in digestion [8]. In other compartments of the body, different CPs participate in tightly regulated processes such as, for instance, the maturation of physiologically active peptide hormones. Moreover, M14 peptidases are therapeutically interesting molecules because of their involvement in acute pancreatitis [9][10][11], cancer [12][13][14], diabetes [15,16], fibrinolysis [17], and inflammation [18].
Carboxypeptidase A structure reveals that the catalytic zinc ion is coordinated by three protein residues and by one water molecule which is displaced upon substrate binding. The substrate is held in the binding pocket through an ion pair between its C-terminal carboxylate group and the guanidinium side chain of Arg145 and two additional hydrogen bonds with Asp144 and Tyr248. The enzymesubstrate interaction is additionally stabilized by the overall hydrophobic character of the binding pocket which reduces competitive solvation by water molecules [19]. The CPA catalytic mechanism has attracted much attention as a model for zinc-dependent proteases and as such routinely investigated [20][21][22][23][24][25]. In the two most widely accepted mechanistic pathways, the zinc ion would play a fundamental role either by activating the amide carbonyl of the substrate or by creating a zinc-water nucleophile that directly attacks the amide group [26]. Chelation of the zinc ion is an important aspect in the development of chemotherapeutic agents targeted to this class of enzymes [27,28] as well as in the development of new drug protective molecules for the 2 Enzyme Research administration of pharmacologically important peptide and protein drugs [29,30].
In the course of our investigations on M14 peptidases and organic synthetic inhibitors [31], we obtained a high resolution CPA-Zn 2+ -citrate ternary complex structure. Such an interaction can be understood from the metal-chelating properties of the citrate anion as citrate, a major component in snake venom, is believed to inactivate metal proteases in the prey assisting in venom toxin movement [32]. Database searches revealed only a few instances in which a biological macromolecule-Zn 2+ -citrate system had been studied at the structural level; surprisingly, none of these involve a biologically active zinc-dependent peptidase. It is interesting to note that in a recent investigation citrate inhibition of carboxypeptidase enzymatic activity has been linked to drug permeation-enhancing properties of the carrier molecule [33]. Thus, the high-resolution structural determination of the CPA-Zn 2+ -citrate ternary complex presented here along with the kinetic evaluation of citrate inhibition of the CPA catalytic mechanism is intended to gain further insights into the interactions between this coligand of pharmaceutical use and the model zinc-dependent intestinal protease.

Protein Crystallization and Structure Solution.
Lyophilized bovine pancreas carboxypeptidase A (Sigma, St. Louis, MO) was dissolved in buffer 0.02 M Tris pH 7.5/1.2 M LiCl and then desalted and concentrated in Centricon centrifugal filter devices (Millipore, Billerica, MA, USA) against 5 mM Tris, pH 7.5. During a screening to analyze the structures of CPA-organic inhibitor complexes, CPA crystals were grown from a 2 μL : 2 μL mixture of enzyme solution (10 mg/mL in 0.02 M Tris, pH 7.5) and precipitant (20% PEG 3, 350, 0.2 M NH 4 Cl, 0.02 M Tris, pH 7.0) by the vapour diffusion method. Crystals suitable for X-ray diffraction analysis were grown in the sitting drop setting in an incubator at the steady temperature of 289 K. The largest crystals were harvested and transferred to a new 2 μL drop containing the reservoir solution plus a millimolar amount of inhibitor solution. As crystal damage was evident, sequential soakings with increasing inhibitor concentrations were performed. Crystals were harvested, briefly bathed in cryoprotectant buffer (i.e., the reservoir solution with added 30% glycerol), flash frozen in the nitrogen stream, and diffracted. Structure determination was made with the molecular replacement method using native CPA (PDB entry 2 ctb) as the model. Extra electron density accounting for three planar carboxylate groups was clearly evident in the region near the catalytic zinc ion. The shape of this density was easily interpreted as a citrate molecule. Sodium citrate, a buffer used to dissolve the inhibitor, would be the probable source of the ligand seen in the electron density maps. No trace of other inhibitor molecules is seen in the maps. The refinement progressed to convergence and reached a good agreement between the model and the experimental data as shown by the statistics listed in Table 1. Protein X-ray diffraction experiments were performed at the X11 and X13 beamlines of the EMBL (European Molecular Biology Laboratory)-Outstation at DESY (Deutsches Elektronen Synchrotron), Hamburg, Germany. X-ray diffraction data was processed with Automar (Marresearch GmbH, Norderstedt, Germany). The CCP4 suite [34] and programs PHASER [35] and REFMAC [36] were used in different stages of structure solution and refinement. MolProbity [37] and PROCHECK [38] were employed to check the protein coordinates against reference geometric values. Model building and map fitting were made with Coot [39]. All the final figures were prepared with Pymol (http://www.pymol.org/).

Kinetic Analysis.
Benzylsuccinic acid (BzlSA), sodium citrate, and other reagents were from Sigma (St. Louis, MO). AAFP substrate was from Bachem (Bubendorf, Switzerland). The working solutions included various concentrations of each compound and one AAFP substrate concentration, usually at the K m value. The final assayed concentrations were stepwise varied between 1 nM and 10 mM (for BzlSA) and between 10 μM and 100 mM (for citrate). One blank well was used as the control without inhibitor, and the experiments were performed at least by duplicate. The enzyme concentration was adjusted for the kinetic measurements to take place within the initial velocity regime. Changes in absorbance due to proteolytic activity were followed continuously in a multimode plate reader Wallac 1420 Victor3 system (PerkinElmer Life Sciences, Waltham, MA, USA). The kinetic measurements were carried out at room temperature using 96-well microtiter plates (Iwaki Co., Japan). Kinetic activity data was then treated with the Graph-Pad Version 5.0 package (GraphPad Software, San Diego, CA, USA). After ensuring a good agreement between the experimental observations and the inhibition model, the K i value was calculated from the IC50 following the procedure of Cheng and Prussoff [40]. The goodness of fit between the inhibition model used and the experimental observations was excellent as indicated by the R 2 parameters, 0.9971 and 0.9822 for BzlSA and citrate, respectively, and by the span of the 95% confidence interval, in which the K i values lie between 0.39-0.67 μM and 3.5-7.0 mM for BzlSA and citrate, respectively.

Analysis of Structural Data.
Although citrate anion is widely used as a buffer in crystal structure determinations, out of the few examples of zinc-dependent proteases bound to this ligand that can be found in the RCSB PDB database, only two hits, namely, human procarboxypeptidase B (entry 1kwm) and porcine procarboxypeptidase A (1pca), were retrieved when searching for carboxypeptidases. Neither of these structures shows citrate in coordination with the catalytic zinc ion, presumably because the presence of the prodomain in both zymogen structures hinders the access to the active site of the enzyme, but the citrate anion resides on the protein's surface exposed to external solvent water molecules. This observation suggests that citrate does bind to exosites in digestive immature proteases, but no structural evidence on its ability to bind to the catalytic machinery of a mature enzyme is available yet. A further query to the RSCB PDB revealed a total of 13 macromolecule-zinccitrate structures, none of which involve digestive enzymes. Therefore, the present crystal structure determination represents the first example of a metal-dependent, fully active, physiologically relevant protease in a ternary complex with citrate. A crystal belonging to the monoclinic space group P2 1 that diffracted beyond 1.70Å was characterized in this study; crystallographic and refinement statistics details are presented in Table 1. The atomic coordinates and structure factors have been validated and deposited with the RCSB PDB with accession code 3kgq.

The
Binding of Citrate to the Catalytic Centre in the CPA Active Site. One citrate anion per carboxypeptidase-Zn 2+ cation was identified from the electron density maps (Figure 1). Citrate binds in an extended conformation to the catalytic centre of the enzyme's active site, where the amide bond is be hydrolyzed, across subsites S1 (defined by residues Arg127 and Glu270, which are involved in the nucleophilic attack of the scissile amide carbon) and S1 (Asn144, Arg145, and Tyr248, which anchor and stabilize the peptide substrate). All but one of the four functional groups from citrate make important interactions with the metal and several ligands from the protein ( Table 2 and Figure 2). The zinc ion is bidentately chelated by citrate oxygen atoms O1 and O2, both atoms lying in the proximity of two zinc ligands from the protein (His69 and Glu72). Oxygen atoms from two different carboxylate groups are hydrogen bonded to Arg127. The nucleophile Glu270 forms a strong hydrogen bond to one carboxylate and the hydroxyl group. An ion pair is formed between oxygen atoms O5 and O6 from citrate and the guanidinium side chain of  Arg145. These atoms make additional interactions with the other S1 residues, Asn144 and Tyr248. The latter residue has been identified as a key player in the CPA catalyzed mechanism by site-specific mutagenesis studies [24]. The conformational change suffered by Tyr248 upon ligand binding is well documented: the aromatic ring switches by 120 • its location between the solvent exposed "open" to the "closed" conformation with the phenolic hydroxyl pointing to the catalytic zinc [41,42]. In this paper, Tyr248 is in the closed conformation expected to occur when the active site of the enzyme is occupied. As seen in other CPA-bound structures, the water molecule is displaced from the zinc coordination sphere by a carboxylate from the ligand and the citrate molecule binds to CPA in a manner reminiscent of the succinate-based ligands such as the benchmark inhibitor benzylsuccinic acid (BzlSA). As will be commented next, while the aromatic ring of BzlSA is buried in the hydrophobic pocket of CPA, the carboxylate group of citrate that occupies the corresponding edge of the molecule adapts its conformation to this specific enzyme's environment.

Citrate Burial in the Hydrophobic Pocket of CPA.
Like citrate, BzlSA chelates zinc and forms an ion pair with Arg145 and hydrogen bonds to catalytically key residues like Tyr248, but the two molecules also display considerable differences in binding to the enzyme (Figure 3). From inspection of the crystal structure in complex to CPA (PDB entry 1cbx), BzlSA extends towards the bottom of the active site cleft with an aromatic ring that mimics a phenylalanine residue. Side chains Leu203, Ile243, Ala250, Gly253, Ile255, and Thr268 define the hydrophobic pocket that harbours the specificity-determinant residue in the M14 peptidase family, which for CPA is Ile255. This hydrophobic pocket is best suited to accommodate an aromatic ring, and Ile255 makes hydrophobic interactions with this part of the inhibitor (Figure 3(a)). The third carboxylate group from citrate is the only one shielded from contact to CPA. Each one of the oxygen atoms of the carboxylate group, O3 and O4, makes strong hydrogen bonds to water molecules, which in turn are hydrogen bonded to other water molecules and amino acid side chains. Thus, this ionisable group lies at least about 3.5Å apart from the wall of the hydrophobic pocket (the closest residue is Ala250) and at more than 7Å from Ile255. Compared to the BzlSA bound structure, the conformation of the latter residue is substantially shiftedas measured by the Cα-Cβ-

Kinetic Assays of CPA Ligands.
Kinetic activity measurements were carried out to determine the ability of citrate to inhibit the CPA catalyzed reaction. The measurements were made in parallel with BzlSA to provide a direct comparison with this standard inhibitor. The nonlinear global fitting of the experimental data to a competitive inhibition model was excellent (Figure 4). Besides, the shape of the citrate inhibition curve resembles that of BzlSA whose K i is in excellent agreement with values obtained in previous works (Table 3). From Table 3, it is evident that citrate performs as a very weak CPA inhibition compared to BzlSA, a likely consequence of the presence of the aromatic moiety in the latter. BzlSA was designed as a by-product analogue, that is, it retains features of the cleaved products of the CPA catalytic reaction [43] in such as way that the aromatic 6 Enzyme Research moiety docks into the hydrophobic pocket of the enzyme, displacing water molecules at the bottom of the active site cleft. This displacement, together with the movement of Tyr248, substantially facilitate the enzymatic activity by creating an overall hydrophobic environment. It is well documented that amino acid substrates and products are very weak CPA inhibitors. For example, L-Phe is, like it has been calculated here for citrate, a 5 mM CPA inhibitor [43]. Although citrate would be easily displaced by the substrate AAFP when competing for binding to CPA in the in vitro experimental assays, a completely different scenario may be possible in vivo such that citrate could be equally well suited as the natural substrates are to bind to the active site of metaldependent proteases.

Conclusion
Citric acid has intestinal absorption enhancing effects and is widely used in drug formulations. Recent evidence demonstrated that the citrate salt of chitosan has a drug penetration enhancement and that this effect is linked to the inhibition of metal-dependent enzymes like leucine aminopeptidase (a membrane protease) and the luminal protease CPA [44,45]. In line with these observations, we report further structural and kinetic evidence of the interaction between CPA and citrate, in which the organic anion chelates the zinc cation at the active site of the enzyme. The citrate mode of binding resembles that of the peptidomimetic substrate analogue benzylsuccinic acid, which also coincides with citrate in being a competitive CPA inhibitor. The K i value for citrate is much weaker than that of benzylsuccinic acid but comparatively similar to that of simple amino acids, suggesting that citrate would compete for the in vivo binding of zinc at the active site of intestinal enzymes.
There is a wide range of digestive protease inhibitors with potential as peptide drug-protecting agents that, like commonly used excipients, may be associated with some drawbacks as elevated costs or risk of toxic side effects [46,47]. Efforts to combine a protease inhibitor and a drug permeation enhancer in one single molecule to increase the stability and absorption of peptide or protein drugs are an increasingly investigated matter [48]. As recently reported [33], citrate, a commonly used excipient, may be an example of such type of molecules. The analysis of the newly determined carboxypeptidase-Zn 2+ -citrate complex structure might help in the structure-guided design of newer drug protecting compounds targeted to metal-dependent proteases.