Comparison of Simulated Drift Rates of Common ALS-Inhibiting Rice Herbicides to Florpyrauxifen-Benzyl on Soybean

Acetolactate synthase(ALS-) herbicides are among the most commonly used sites of action (SOA) in rice production. Many herbicides used in rice can cause carryover to soybean,which is commonly grownnear to or rotatedwith rice. Florpyrauxifen-benzyl (Rinskor Active) brings an alternative SOA to rice production.The objective of this study was to compare the effects of simulated drift rates of florpyrauxifen-benzyl to commonly used ALS-inhibiting rice herbicides on soybean. A field study was conducted at two locations examining fiveALS-inhibiting rice herbicides as well as florpyrauxifen-benzyl at a 1/20x and 1/80x simulated drift rate. Crop injury, height, and yield were evaluated at 14, 21, and 35 days after treatment (DAT). Florpyrauxifen-benzyl and bispyribac showed high injury levels at both drift rates. At 35 DAT florpyrauxifen-benzyl caused 76% and 17% visible damage to soybean whereas bispyribac caused 35 and 9% injury at 1/20x and 1/80x, respectively. These treatments resulted in a reduction in soybean height and yield. Although this alternative SOA herbicide in rice may be effective for weed control, our research demonstrates it to be injurious to soybean at both drift rates tested. Thus, proper precautions should be taken to avoid injury by ensuring that the label is followed.


Introduction
Drift from auxin-type herbicides is currently a major concern within the agricultural community [1].Drift can be categorized into either physical or vapor drift.Physical, or particle, drift occurs at the time of application and is influenced by wind speed [2].Additionally, improper application speed, height above target, and nozzle selection can also contribute to the occurrence of physical drift [3].When examining aerially applied herbicides, height above the target would be the primary factor contributing to drift.Vapor drift, conversely, is primarily a function of volatilization that takes place after spray particles reach their intended site.This form of drift can be influenced by various abiotic factors, such as temperature and relative humidity [4,5].Increased temperature and low humidity tend to intensify the risk for volatility to occur by increasing the amount of atmospheric space for evaporation to take place [4].Auxin-type herbicide residues, such as 2,4-D and dicamba, are known to be difficult to clean from spray equipment, and small amounts of these compounds could be inadvertently applied to susceptible crops if the same equipment was subsequently used to treat non-dicamba or non-2,4-D-resistant crops [6].Finally, in regions where 2,4-D or dicamba are used frequently and over large areas, herbicide residues can accumulate in the atmosphere and return to fields as precipitation at concentrations high enough to cause injury to susceptible crops [7,8].
In the mid-southern United States (US), soybean is frequently grown in close proximity to rice or rotated with rice [9].The overreliance on herbicides with the same site of action (SOA), such as acetolactate synthase-(ALS-) inhibitors, has led to many weed species evolving resistance to these herbicides [10].For example, in the US, there are ten reported weeds, in rice, that are resistant to ALSinhibitors [11].The continued evolution of herbicide-resistant weeds is only increasing further with more cases being reported.Therefore, a new herbicide SOA is needed in rice production that will limit not only the frequency of resistance in weed species, but also any injury to soybean.The introduction of florpyrauxifen-benzyl (Dow AgroSciences LLC, Indianapolis, IN) will be a new SOA for weed control in rice.Florpyrauxifen-benzyl (Rinskor Active) is a new active ingredient in the arylpicolinate herbicide family and represents the second herbicide (the other one being halauxifenmethyl) in the new structural class of synthetic auxins having a unique receptor binding activity [12,13].Members of this family exhibit distinctive characteristics for synthetic auxins by providing broad-spectrum postemergence activity on broadleaf, grass, and sedge species at low use rates.The addition of the compound to the US rice marketplace will provide an alternative SOA, thereby providing effective control of propanil-, quinclorac-, clomazone-, and ALS-resistant barnyardgrass, ALS-resistant rice flatsedge, smallflower umbrella sedge, yellow nutsedge, and other troublesome weeds in rice cropping system [12].Furthermore, previous research supports a relatively short (≤60 days) plant-back interval for soybean after florpyrauxifen-benzyl application compared to other herbicides commonly used in rice [14].
There is little research, however, on risk for injury to soybean caused by florpyrauxifen-benzyl drift [15].Soybean, cotton (Gossypium hirsutum L.), and grain sorghum (Sorghum bicolor) are among the crop species most susceptible to synthetic auxin herbicides, such as dicamba, 2,4-D, and florpyrauxifen-benzyl.Previous research has reported numerous consequences of dicamba drift onto non-dicambaresistant soybean, such as reduced growth, fewer seeds per pod, lower seed quality, maturity delays, and pod malformation [16][17][18][19][20]. Symptomology can vary from chlorosis of the terminal buds, cupping or crinkling of canopy leaves, and leaf or stem epinasty.Higher rates can even result in stem cracking, terminal death, or plant death [17,21,22].The risk of crop injury and potential yield loss will perhaps be greatest to growers who choose not to be aware of crops in surrounding fields or who choose not to use resistant varieties in regions where these varieties and associated herbicide programs are widely adopted by neighboring farmers [23].Thus, the objective of this study was to determine the effects of various drift rates of florpyrauxifen-benzyl relative to commonly used ALS-inhibiting herbicides on soybean.

Methods
A field experiment was conducted in 2016 at the Arkansas Agricultural Research and Extension Station (AAES) in Fayetteville, Arkansas, and at the Rice Research and Extension Center (RREC) near Stuttgart, Arkansas.Each experimental plot contained four rows spaced 91 cm apart resulting in an overall plot size of 3.6 m wide by 6 m long.At both sites, a non-ALS tolerant variety was planted.Pioneer 95L01 was planted on May 5 and May 11 at AAES and PTRS, respectively.Soybean was planted at a 2-cm depth at 120,000 seed ha −1 using a tractor-mounted John Deere 7200 MaxEmerge planter (John Deere Seeding Group, Moline, IL).Plots were kept weed-free utilizing labeled herbicides and occasional hand weeding.All herbicide treatments were applied at the V3 growth stage to the two center rows with a CO 2 -pressurized backpack sprayer fitted with 110015 AIXR nozzles (Teejet Technologies, Springfield, IL) calibrated to deliver 140 L ha −1 at 4.8 km hr −1 .
The experiment was arranged as a randomized complete block design with a two-factor factorial treatment structure and four replications.The first factor was herbicide treatment.Treatments consisted of five ALS-inhibiting herbicides: bispyribac (Regiment; Valent USA Corporation, Walnut Creek, CA) with a 1x rate of 27 g ai ha −1 , penoxsulam (Grasp5, Dow AgroSciences LLC, Indianapolis, IN) with a 1x rate of 35 g ai ha −1 , halosulfuron (Permit; Gowan Company, Yuma, AZ) with a 1x rate of 40 g ai ha −1 , orthosulfamuron (Strada, Nichino America Inc., Wilmington, DE) with a 1x rate of 69 g ai ha −1 , and imazosulfuron (League, Valent USA Corporation, Walnut Creek, CA) with a 1x rate of 336 g ai ha −1 , as well as florpyrauxifen-benzyl (Rinskor Active) with a 1x rate of 30 g ai ha −1 , and a nontreated control.The second factor was simulated drift rates.Each herbicide was applied at two simulated drift rates of 1/20x and 1/80x with florpyrauxifenbenzyl, bispyribac, and penoxsulam containing a 1% v/v of methylated seed oil (MSO), halosulfuron containing 1% v/v crop oil concentrate (COC), and orthosulfamuron and imazosulfuron containing 0.25% v/v nonionic surfactant (NIS).The simulated drift rates were made using serial dilutions from a 1x stock solution of each herbicide.
Data collection included estimates of visible injury on a scale of 0 to 100%, with 0% representing no injury and 100% representing complete crop death at 14, 21, and 35 days after treatment (DAT).In addition, crop height to the terminal at each rating was measured.Grain yield was also collected by harvesting the two treated rows and correcting for moisture at 13%.Site, herbicide treatments, and drift rates were treated as fixed factors and replications were treated as random.Data were subjected to analysis of variance (ANOVA) in JMP Pro 12 (JMP Pro 12, SAS Institute Inc., Cary, NC).Where the ANOVA indicated significance, means were separated using Fisher's protected least significant difference test ( = 0.05).

Results and Discussion
There was no significant effect or interaction with site; thus data were pooled over sites.There was a significant interaction ( = 0.02) between drift rate and herbicide treatment for all evaluations (Table 1).All treatments resulted in injury 14 DAT, with the 1/20x drift rate having higher injury than the 1/80x drift rate, except for halosulfuron.Regardless of the drift rate, florpyrauxifen-benzyl was more injurious to the soybean than the other herbicides within a drift rate.For example, injury caused by florpyrauxifen-benzyl at the 1/20x and 1/80x drift rates was 71 and 31% at 21 DAT and 76 and 17% at 35 DAT, respectively.Likewise, soybean height was reduced by both rates of florpyrauxifen-benzyl at 14 and 21 DAT.Bispyribac also produced a higher level of crop injury than the  other ALS-inhibiting herbicides at 21 and 35 DAT at the 1/20x drift rates (35% injury), albeit lower than florpyrauxifenbenzyl.Most ALS-inhibiting herbicides applied at a 1/20x rate caused initial stunting of soybean at 14 DAT, but by 35 DAT, most had recovered to the point of being comparable in height to the nontreated control.The high degree of crop injury and stunting had a significant effect on yield.The nontreated control yielded 2960 kg ha −1 whereas soybean treated with florpyrauxifenbenzyl at a 1/20x and 1/80x rate yielded 540 and 2,220 kg ha −1 (82 and 25% yield loss), whereas plants treated with bispyribac, the most injurious ALS herbicide, had yields of 1280 and 3030 kg ha −1 (57% and 0% yield loss) at comparable drift rates.The other four ALS-inhibiting herbicides did not differ in yield from the nontreated control at either drift rate.Although florpyrauxifen-benzyl may be highly effective in weed control [24], this new SOA will need to be used with caution and following label application recommendations when soybean is in close proximity to rice as there appears to be slightly greater risk of crop injury than with currently used ALS rice herbicides.
Although florpyrauxifen-benzyl will provide midsouthern rice growers with an alternative herbicide SOA that is capable of achieving a high level of weed control [24], it will be injurious to soybean at a 1/80x and 1/20x drift rate.Furthermore, regardless of drift rates, soybean yield was reduced in comparison to the nontreated control.Caution should be exercised when applying florpyrauxifen-benzyl when soybean is in close proximity to rice.

Conclusions
This study compared the effects of simulated drift rates of florpyrauxifen-benzyl to commonly used ALS-inhibiting rice herbicides on soybean and found that at 1/20 and 1/80 of the 1x rate, florpyrauxifen-benzyl caused a significant increase in crop injury and crop height and yield reduction.Previous research has documented many consequences associated with auxin herbicide drift onto soybean, such as fewer seeds per pod, lower seed quality, pod malformation, and reduced yield [16][17][18] as well as reduced growth when exposed to drift rates of auxin herbicides, all of which were documented in this study.New herbicides are needed, however, to enhance SOA diversity, which would lessen the risks of herbicideresistance evolution [25] and could improve weed control.However, growers should exercise caution and strictly follow label recommendations when applying florpyrauxifen-benzyl to a rice field where soybean is adjacent.In addition, soybean cultivars expressing tolerance to dicamba or 2,4-D will not protect against off-target movement of this herbicide (trademark of The Dow Chemical Company ("Dow") or an affiliated company of Dow).

a
Means within columns followed by different letters are significantly different using Fisher's protected least significant difference ( = 0.05).