Growth and Fruit Yield of Okro as Influenced by Genotypes and Mulch in the Guinea Savannah Conditions of Ghana

The experiment was carried out to assess the suitability of different mulch materials in enhancing the growth and fruit yield of okro. Ten okro genotypes were evaluated in a split plot design with 3 replications. Three treatments of mulch (black plastic, grass, and no mulch) represented the main plots with the genotypes as the subplots. The result indicated significant (P < 0.05) genotypic variability among the genotypes for all parameters except plant girth. However, genotype andmulch interaction was not significant. The genotype Sasilon had the tallest plants (82.6 cm) and the highest fruit yield under allmulch conditionswhileKoni had thewidest fruits (34.1mm) with TZ SMN 10-3 having the longest fruits (16.11 cm). Number of fruits per plant ranged from 30 to 11 with an average of 21. Mulching significantly (P < 0.05) influenced all parameters except fruit width andmean fruit weight. Plastic mulched plots had the greatest heightswhile nomulching had the least.Thehighest average yieldwas obtained under plasticmulch (3.49 t/ha) which was 4.2% higher than grass (3.34 t/ha) and 11% higher than no mulch (3.11 t/ha). The study has shown that mulching with black plastic or grass ensures vigorous growth and improves the fruit yield of okro.


Introduction
Okro (Abelmoschus esculentus (L.) Moench) belongs to the plant family Malvaceae and is the most common and widely cultivated crop within this family.It is an economically important vegetable crop cultivated in the tropics and subtropics of the world [1].Okro is an important vegetable crop cultivated for its fresh immature fruits and sometimes young tender leaves.The immature fruits contain appreciable levels of vitamins and minerals and essential fatty acids such as linoleic acid [2].It is an affordable source of dietary fibre, protein, and carbohydrates and constitutes a major component of many Ghanaian dishes: soups, stews, and salads.The consistency of many soups and sauces can be increased by adding the extract obtained from boiling the immature fruits.Okro has many medicinal properties and the mucilage has been used as a plasma replacement [3].
Okro production in the rainy and dry seasons (along river banks) serves as a source of employment for many people in Ghana.However, the cultivation of the crop is affected by many biotic and abiotic factors such as pests and diseases, low soil fertility, lack of improved seeds, and climate change.Climate change and its concomitant environmental stress will consequently reduce crop productivity especially in developing countries where crop production is mainly rainfed [4].Intermittent or unpredictable drought and high temperatures during the growing season are important environmental challenges affecting crop productivity and food security worldwide [5].
Water availability is expected to be sensitive to climate change and severe water-stress conditions will affect crop productivity, particularly that of vegetables which generally consist of more than 90% water [6].Reduced precipitation, in combination with elevated temperatures, could cause reduction of irrigation water availability and increased evapotranspiration, leading to severe crop waterstress conditions which reduces flower set and increases the rate of floral abscission.Evapotranspiration as influenced International Journal of Agronomy by high temperatures has been found to be a major factor contributing to declining yields of vegetable crops under irrigated conditions in Ghana [7].Emphasis should therefore be on developing production systems for improved water use that are adaptable to the hot and dry conditions of northern Ghana.Earlier studies have indicated that cropping systems that utilize different degrees of soil cover reduce soil evaporation water loss and enhance crop water productivity [8,9].The use of mulches has been found to cause 34-50% reduction in soil water evaporation compared with bare soil [10].
Mulch is any material derived from either organic or inorganic sources placed on the surface of the soil as a protective cover.Different types of mulch materials have been used to conserve moisture to enhance the growth and yield of several crops such as groundnut, soybean, sesame, and sunflower [11][12][13][14].Mulching helps to conserve soil moisture, moderate soil temperature, and prevent soil runoff and erosion [15].The protective cover of mulches serves as a barrier and prevents direct contact between fruits and the soil.Mulching with black plastic or opaque materials prevents sunlight from reaching the soil surface which reduces weed growth and promotes soil warming and early crop establishment [16].Mulching with coloured plastic has been found to prevent insect and disease damage on several crops [17].The use of organic materials as mulch adds nutrients to the soil as they decompose and enhance crop growth and yields [16].It also improves the physical, chemical, and biological properties of the soil.Although the importance of mulching cannot be overemphasized, however, there is very little information on the influence of mulch materials on okro production in the Guinea Savannah agroecological zone of northern Ghana where rainfall is unevenly distributed.There is therefore the need to assess the suitability of different mulch materials in enhancing the growth and yield of okro for intensive production.This study was carried out to assess the effect of different mulch materials on the growth and fruit yield of okro genotypes in northern Ghana.

Study Area.
The experiment was conducted during the 2014 cropping season at the research field of the Savanna Agricultural Research Institute (SARI), Nyankpala in the Guinea Savannah zone of Ghana (N 09 ∘ 23  21.4  , W 001 ∘ 00  13.4  ).The study area is characterized by a monomodal rainfall with an average annual rainfall of about 800 to 1200 mm⋅yr −1 which peaks between August and September and ends in October.The soils belong to the Kumayili series and are commonly classified as Ferric Luvisols.Table 1 describes some physical and chemical properties of the study site at a depth of 0-15 cm.

Experimental Design and Treatments.
The experiment was conducted in a split plot design with three replications.Three treatments of mulch (black plastic, grass mulch, and no mulch) represented the main plots with the 10 okro genotypes as the subplots.The land was ploughed with a disc-plough  Reduced model fitted: Yld t/ha where  0 is intercept,  1 - 3 are regression coefficients or slopes for the three predictor variables (SSI, FSI, and FrtNo P),  is residual or error term, Yld t/ha is yield (t/ha), SSI is shoot size index (ratio of mean plant height to mean plant girth), FSI is fruit size index (ratio of fruit length to fruit width), and FrtNo P = fruit number per plant.

Influence of Mulch on Growth and Yield Characteristics of Okro.
The results indicated that all parameters measured, namely, plant girth, plant height, fruit length, fruit number, number of fruits/plant, and fruit yield, were significantly ( < 0.05) influenced by mulch except fruit width and mean fruit weight.However, genotypes and mulch interaction was not significant for any parameter (  highest yields of okro and eggplant cultivars when they were mulched with plastic and grass mulches as compared with no mulch.The findings from the present study could be attributed to prolonged availability of soil moisture for crop growth during intermittent drought periods observed in the growing season.Mulching also suppressed weed growth and competition for limited available resources such as nutrients and water.This result is in agreement with Lamont [22] who reported that mulching enhanced soil hydrothermal regime and improved vegetative growth and fruit yield of tomato plants as compared to bare soil.Teame et al. [9] also observed that mulching significantly improved soil moisture content and influenced grain filling in sesame as compared to no mulching.The mulches protected the soil from the direct impact of rains, drying winds, and heat which compact the soil.Moisture stress during periods of intermittent drought was not experienced in the mulched plots which might have accounted for the improved performance of the okro genotypes.

Genotypic Variability among Okro Genotypes.
The results indicated significant ( < 0.05) genotypic variability among the okro genotypes for the parameters measured except plant girth (Table 3).Fruit length had highly significant positive association with fruit size index (FSI) but significant negative correlation ( = −0.54)with fruit width.Fruit width had significant positive associations ( = 0.44 and 0.53) with fruit yield and SSI but had significant negative association with FSI.Fruit number per plant showed highly significant positive associations ( = 0.82 and  = 0.36) with fruit yield and SSI.Fruit yield showed highly significant positive correlations ( = 0.63) with SSI.Agyare et al. [24] found significant positive associations ( = 0.51 and  = 0.71) between plant height and plant girth and fruit yield and fruit width, respectively, in pepper.These traits can be used as indicators for selecting okro genotypes with desirable attributes especially fruit yield.

Multiple Linear Regression Analysis.
Multiple linear regression analysis revealed that the full model was highly significant (-statistic: 37.90) with the three predictor variables explaining 79% (adjusted -squared = 0.79) of the variability in fruit yield of okro (Table 5).The regression coefficients for fruit number per plant (0.21) and shoot size index (0.53) were significantly different from zero ( < 0.01).
The regression coefficient of fruit size index was however not significant ( > 0.05) and was eliminated from the model.The reduced model shows that the two predictor variables retained in the model explained 78% of the variability in the fruit yield of okro.The reduced model was preferred over the full model since its adjusted -square was comparable to that of the full model and it is simple to use because of the low number of predictor variables in the model.The reduced model shows that increasing the fruit number per plant by one unit would result in an increase of 0.20 t/ha of okro fruit yield, while holding the shoot size index constant.Similarly, when the shoot size index increases by one unit, the fruit yield is predicted to increase by 0.61 t/ha, while holding the fruit number per plant constant.This model has revealed that fruit number per plant and shoot size index could be a useful tool in predicting the fruit yield of okro genotypes.Future breeding programs aimed at okro yield improvement could be focused on improving the shoot size index and fruit number per plant.

Conclusion
Mulching resulted in better growth and yield of the different okro genotypes compared with the bare soil.Significant genotypic variability was observed among the okro genotypes for most of the parameters studied particularly fruit yield.Genotype and mulch interaction was not significant for any parameter suggesting that the performance of genotypes will not be affected irrespective of the type of mulch materials used.Plastic mulch enhanced the growth and yield of okro better than grass mulch as well as no mulching.Correlation analysis indicated that plant height and fruit width had significant positive association with fruit yield.A reduced fitted linear regression model revealed that 78% of the variability in fruit yield of okro was explained by fruit number per plant and shoot size index.Future fruit yield improvement programs could therefore be centered on improving fruit number per plant or the shoot size index of okro.

Table 1 :
Soil physical and chemical properties of the study site.
[18]black plastic (thickness 1.25 mm) was used to cover the experimental plots before planting.Planting holes were made on the plastic mulch for planting the seed.The grass (straw) mulch was applied to the plots two weeks after seedling emergence at a rate of 4 t/ha.The okro genotypes evaluated were Koni, Sasilon, ML OK 10, ML OK 16, ML OK 35, ML OK 37, TZ SMN 10-3, TZ SMN 86, TZ SMN 98, and Ex Makutopora.Okro seeds were soaked in water overnight before they were planted at spacing of 0.75 m between ridges and 0.50 m between plants.The plants were thinned to two plants per hill at two weeks after emergence.Each plot consisted of 4 rows measuring 3 m long and 3 m wide.Agronomic practices such as fertilizer application, insect pest, and diseases control were carried out appropriately to ensure good crop establishment.2.3.Data Collection and Analysis.Agronomic data were collected on plant height (cm), plant girth (mm), fruit length (cm), fruit width (mm), fruit number per plot, number of fruits/plant, mean fruit weight, and fruit yield (t/ha).The measurements were taken on 4 tagged plants that were randomly selected from the two middle rows.The data were analysed using GenStat statistical package version 12.0[18].Full model fitted: Yld t/ha =  0 +  1  SSI +  2  FSI +  3  FrtNo P +

Table 3 :
Growth and yield characteristics of okro genotypes evaluated under different mulch materials.
Means followed by the same letter within each column indicate no significant difference between treatments by Duncan's multiple range tests ( < 0.05).

Table 2
[21]kro genotypes grown under black plastic mulch had the widest plant girth (20.25 mm) which was significantly different from plants grown under grass mulch (13.15 mm) and bare soil (12.98 mm) (Table3).The highest plant height (90.as compared to no mulching.Mean fruit weight was not significantly affected by mulching.The highest fruit yield was obtained under black plastic mulch (3.49t/ha) which was 4.2% higher than grass mulch (3.34 t/ha) and 11% higher than no mulch (3.11 t/ha).This observation corroborates earlier work by Pandita and Singh[21]who recorded the

Table 4 :
Pearson correlation analysis for eight traits obtained from 10 okro genotypes under three mulch treatments.