Gene-Environment Interaction Research and Transgenic Mouse Models of Alzheimer's Disease

In the published version of this paper, the following corrections should be introduced: (1) On the first page, it should be mentioned that " L. Chouliaras and A. S. R. Sierksma are joint-first authors. " (2) At page 11, Section 7 " Electromagnetic field exposure " , line 41: it was incorrectly stated that ". . ., decreased transthyretin levels have also been found in blood serum of long-term wireless phone. " The correct text should read ". . ., increased transthyretin levels have also been found in blood serum of long-term wireless phone. "


Introduction
Alzheimer's disease (AD) is the most common form of dementia, characterized by an initial loss of short-term memory, followed by a progressive impairment in multiple cognitive domains. The estimated lifetime risk for developing AD is about 20% for women and 10% for men aged above 65 [1]. The pathology of AD is characterized by an accumulation of misfolded proteins, oxidative damage, and inflammatory changes ultimately resulting in region-specific loss of synaptic contacts and neuronal cell death [2]. Current biological theories on the etiology and pathology of AD posit central roles for age-related molecular and cellular aberrations that induce an imbalance in the production, cleavage, and clearance of amyloid-β (Aβ), hyperphosphorylation of the tau protein, and aberrant apolipoprotein E (APOE) function in the aging brain [1]. Several genetic risk factors have been linked with an increased risk of developing AD, such as mutations in the amyloid precursor protein (APP) and presenilin (PS) 1 and 2 for the familial cases of AD (FAD), as well as the APOE4 allele for the sporadic late-onset form of AD (LOAD). Several new genetic findings derived from powerful genome-wide association studies (GWAS; see below) have confirmed that AD is a polygenic disorder. The genes identified in these studies may enlighten unknown biological pathways involved in AD [3].
Furthermore, various environmental exposures have been found to modify the risk of AD, such as diet and nutrition, physical exercise, exposure to metals, and brain trauma. Comorbidities, such as vascular disorders or depression, could also be of considerable importance, since these have also been suggested to contribute to the risk of AD. Recent evidence indicates that more attention should be paid to the role of the environment and its interactions with underlying genetic susceptibility in triggering diseaserelated phenotypes [4]. The gene-environment interaction (GxE) approach differs from the linear approach of either genetic or environmental effects by positing a causal role not only for either genes or environmental exposures in isolation, but for their synergistic participation in leading to a certain phenotype (here AD), where the effect of one is conditional for the other [5][6][7]. Where epidemiological studies on AD may reveal statistical evidence for GxE in the onset and course of AD, animal research can be instrumental in studying the underlying biological mechanisms.

1.1.
Objective. The objective of this review is to give an overview of the available transgenic mouse studies on AD, specifically addressing the concept of GxE. We start with a brief description of the various genetic and environmental risk factors of AD, and the different available transgenic mouse models of AD. The main part of the paper describes the effects of several environmental exposures on AD-related phenotypes. These sections begin with a brief description of the epidemiological evidence in AD (when available from meta-analyses) and continue with describing the findings from experimental animal studies in which the environmental factor was manipulated in AD transgenic mice and, when performed, in wild-type (WT) mice. Thereafter, we discuss the strengths and limitations of these studies, and we end with identifying future challenges and prospects.

Genetics of AD.
Twin studies on AD have shown a heritability of 60%-80% and a concordance of 18% up to 83%, depending on for example, the population and age of the subjects investigated. Thus, both heritable and nonheritable factors play an important role in AD's age of onset, risk and etiology [8][9][10][11]. Several genetic risk factors have been linked to AD. Mutations in APP, PS1, and PS2 genes have consistently been associated with early-onset FAD. Also for LOAD several susceptibility loci have been linked with risk for AD, such as the gene encoding for the APOE4 allele or loci in the clusterin (CLU), phosphatidylinositol binding clathrin assembly protein (PICALM), complement receptor 1 (CR1), BIN1 (bridging integrator, amphiphysin) genes, a locus near the BLOC1S3 (biogenesis of lysosomal organelles complex1, subunit 3), and MARK4 (microtubule affinityregulating kinase 4) genes [3,[12][13][14]. Other susceptibility loci have also been associated with AD (see [12,15], http://www.alzgene.org/).

Environment and AD.
Although a range of environmental exposures have been linked to AD, well-replicated and meta-analyses' evidence for the involvement of clear environmental factors in AD is sparse. Recent studies, however, have shown that dietary factors, such as exposure to a Mediterranean diet, fish and high omega-3 diets, cigarette smoking, head trauma, infections, systemic inflammation, and metal and pesticide exposure can significantly alter an individual's risk of developing AD. In addition, psychosocial factors such as education, social network, leisure activities and physical activity, chronic stress, and depression also seem to be connected to the risk of developing AD [16][17][18]. Somatic factors that are under the direct influence of environmental exposures, such as blood pressure, obesity, diabetes mellitus, cardio-and cerebrovascular diseases, and hyperlipidemia, have additionally been implicated in AD etiology [16,18].

Gene-Environment
Interactions and AD. The field of GxE research appears very promising for psychiatry and neuroscience, albeit still little investigated in AD [19]. The notion of potential existence of GxE in AD has substantial impact on the interpretation of reports on genetic and nongenetic contribution to this disorder. Reported contributions of environmental and genetic factors to disease risk can be misleading, since they represent the environmental exposure in relationship with the genetic susceptibility or resilience to it [6]. Thus, the advantage of the concept of GxE is that it includes the genetic control of sensitivity to the environment. Additionally, the genome-wide genetic findings identify associations that also include underlying GxE [6]. In fact, evidence for GxE in AD has recently started to accumulate. For example, an interaction between the APOE4 allele and cholesterol levels has been shown to increase the risk of AD [20,21]. Significant statistical interactions were also found between moderate consumption of alcohol and the APOE4 genotype, as well as for smoking and the APOE4 genotype [22,23]. Furthermore, an interaction with this risk genotype and social factors, such as cohabiting with a partner has been found; APOE4 carriers who lost their partner before midlife showed an increased risk of developing AD, compared to married or cohabiting people [24].
These epidemiological studies indicate that it makes sense to focus future clinical AD studies on measuring both genes and environment and analyzing possible interactions, given that certain environmental factors may only affect a phenotype when the person is genetically endowed. A major drawback of epidemiological clinical studies is that they may indicate merely statistical interactions and thus cannot easily decipher the biological mechanisms that underlie the observed statistical interactions. Other major obstacles in clinical studies are the heterogeneity of the study population and co-occurrence of various environmental exposures in the same individuals. Experimental animal research has the advantage of enabling strict control of genetic and environmental variables. Recent advances in transgenesis allow altering specific genes in isolation, and in a time-and regionspecific manner. As such, transgenic mice form a useful tool to study the effects of genetic and environmental variations and to identify the biological mechanisms that underlie the statistical GxE interactions observed in epidemiological studies (see Figure 1). Figure 1: Research in Alzheimer's disease (AD) uses both clinical (human) and preclinical (mouse) methods to elucidate the underlying mechanisms of AD etiology. Epidemiological findings such as genetic and environmental risk factors can provide tools for investigating their effects on AD etiology separately in mouse models of AD. In this paper it is, however, postulated that AD research should move towards a gene-environment (GxE) interaction approach, so that the synergistic participation of genes and environment can be scrutinized. Genes in the dashed box represent those genes found to be implicated with Alzheimer's disease etiology in humans, while genes in the solid box resemble the genes that are currently used in mouse models of Alzheimer's disease. APOE4: Apolipoprotein ε4; APP: amyloid precursor protein; CLU: clusterin; EMF: electromagnetic field; PICALM: phosphatidylinositol-binding clathrin assembly protein; PS1: Presenilin 1; PS2: Presenilin 2.

Transgenic Mouse Models of AD.
Without the intention of giving a full overview of the available AD mouse models, some details on the types of transgenic mice that are discussed in the present paper can be found in Table 1. Information on the promoters used for the transgenic construct, and further details on genetic background are not further discussed here as these aspects lie outside the scope of this paper.
It is noteworthy that the Aβ sequence of WT rodents has a three amino acid difference compared to humans, making it less likely to aggregate and deposit into amyloid plaques [25]. Therefore, to study Aβ aggregation and plaque formation in rodents it is necessary to manipulate them genetically [25]. Most transgenic mouse models focus on overexpressing human APP, PS1, tau, or APOE variants.

Human Studies of Chronic Stress.
Chronic stress has been implicated in the etiology of AD. The likelihood of developing AD has been shown to increase by a factor 2.7 with the personality trait distress proneness [25,48,49]. Moreover, AD patients show elevated plasma cortisol levels [50,51] with higher levels of plasma cortisol being associated with a more rapid disease progression and cognitive deterioration [51,52].
Evidence from studies over the last 20 years indicates that major depression may serve as a risk factor for developing AD [62][63][64][65][66][67][68][69]. A lifetime history of depressive episodes doubles the chance of developing AD [70]. Interestingly, patients with major depression show a cerebrospinal fluid (CSF) profile of Aβ-species that resembles the profile seen in AD. They display decreased levels of Aβ42 and a decreased Aβ40 : Aβ42 ratio [71], which are considered putative biomarkers for AD [72]. In addition, the severity of depression correlated with binding of 2-(1-{6-[(2-{ 18 F}Fluoroethyl)(methyl)amino]-2-naphthyl}ethylidine)malononitrile, also known as FDDNP, a tracer that binds to plaques and tangles, in the temporal lobe [73]. Moreover, more plaques and tangles in the hippocampus as well as a more rapid cognitive decline have been observed in AD patients with a lifetime history of major depression compared to patients without such history [74]. In contrast, others have suggested that major depression does not function as an independent risk factor for AD, but should merely be viewed as an AD prodrome [63,75,76].

Animal Studies of Chronic Stress.
Several paradigms have been used to model the effects of chronic stress in mouse models of AD. The paradigms that have been applied most frequently are chronic isolation stress and chronic restraint or immobilization stress. Table 2 summarizes the current evidence for effects of stress exposure in transgenic mouse models of AD.
Chronic isolation stress by subjecting mice to either 3, 5, or 6 months of social isolation from weaning, has thus far only been used in the Tg2576 mouse model of AD [77][78][79]. This resulted in elevated levels of soluble Aβ40 and Aβ42 up to 59% and increased plaque deposition in the hippocampus and the neocortex [77,78]. Moreover, this stress exposure paradigm caused a rise in basal plasma corticosterone levels, paralleled with an increased expression of the glucocorticoid receptor (GR) and corticotropin-releasing factor (CRF) receptor 1 [78]. In addition, impaired contextual memory and decreased cell proliferation in the hippocampal dentate gyrus was observed. Interestingly, the effects of isolation stress on memory deficits and cell proliferation in the dentate gyrus could be prevented by a 14-day treatment of fluoxetine [77].
Another widely used stress paradigm is restraint stress. Acute short-term restraint stress elevated intracerebral interstitial Aβ levels in Tg2576 mice [79] and stress-induced corticosterone release in APPswe mice [81]. Administering CRF or a CRF-antagonist indicated that the interstitial rise in Aβ depended on CRF levels [79]. Acute restraint stress furthermore resulted in a 175% increase in blood glucose levels in APPswe mice, suggesting a wide impact on metabolism [81].   [47] Chronic restraint stress has so far been performed in 3 different mouse models of AD: APPV717I-C100, Tg2576, and PS1-L286V mice. Applying chronic restraint stress to APPV717I-C100 and Tg2576 mice generally resulted in an increased Aβ plaque load, increased Aβ40 and Aβ42 levels, increased tau phosphorylation and increased basal plasma corticosterone levels [82][83][84]. Chronic restraint stress applied to APPV717I-CT100 mice additionally induced cognitive impairment as measured for example by using cued food, that is, powdered chow mixed with a certain aroma, in the social transfer of food preference task [82]. Chronic restraint stress has also been associated with neuropathological alterations in AD mouse models. PS1-L286V mice exposed to chronic restraint stress displayed elevated numbers of degenerating neurons and a decreased number of proliferating cells in the hippocampus as compared to nonexposed mice [84]. Chronic restraint stress in APPV717I-CT100 mice caused elevated numbers of pyknotic cells in the hippocampus [82] and reduced dendritic arborization of cortical neurons in Tg2576 mice [83].
Another method to assess the effects of stress is by mimicking the physiological stress response by administering synthetic glucocorticoids, such as dexamethasone, for 7 days. Application of this approach in 3xTg mice resulted in elevated Aβ-and tau-immunoreactivity in the hippocampus, amygdala and neocortex and increased levels of insoluble Aβ40 and Aβ42 and total APP, β-site of APP cleaving enzyme (BACE1) and APP fragment C99 levels in brain homogenates [85].

Human Studies of Environmental Enrichment.
A reduced risk for developing and a slower rate of cognitive decline have been observed in people having a greater purpose in life and higher levels of physical activity [86,87].

Animal Studies of Environmental Enrichment.
In the field of animal research, the environmental enrichment (EE) paradigm is frequently used to manipulate physical activity and social interactions. By introducing mates (social interaction) and/or toys (physical activity) into the cage of the rodent [88], this paradigm stimulates cognition as well as sensory and motor behavior with concomitant intracerebral cellular and molecular changes [89,90]. To examine the effect of EE in AD several different paradigms have been imposed on various mouse models of AD.   Table 3 summarizes the effects of EE in transgenic mouse models of AD.

APP Mice.
EE, in terms of housing multiple mice in a larger cage with platforms, running wheels, toys, and other novel habitats, for a period of 6 months improved cognition in a battery of tests such as Morris water maze (MWM), circular platform, platform recognition and radial arm water maze, despite signs of stable Aβ deposition in 16-monthold APPswe mice [91]. EE for 4 months in 5-month-old TgCRND8 mice did not significantly alter soluble levels of Aβ in the brain or the blood, but did enhance mRNA expression of angiogenic genes [92]. EE in this mouse model attenuated age-related reductions in cell proliferation, neurogenesis and synaptic plasticity [93], while the same paradigm in another laboratory elevated Aβ plaque load without compromising behavioral phenotypes such as feeding and drinking pattern, grooming, locomotion or cognition [94,105]. In an attempt to disentangle the exact components of EE that influence phenotypes in APP mutant mice, Wolf et al. [96] exposed APP23 transgenic mice to either an enriched environment or unlimited access to a running wheel and compared both conditions with standard housing. EE had differential effects upon improving performance in the MWM as compared to the increased physical activity and standard housing groups, however, no differential effects on plaque load in the neocortex or hippocampus were found [95,96]. Moreover, mice exposed to EE exhibited signs of increased hippocampal neurogenesis and neurotrophic support [95,96].

APP/PS1
Mice. When comparing social interaction and physical activity, differential effects of EE can be observed on learning and memory processes, Aβ plaque load and synaptophysin immunoreactivity of 9-month-old APP/PS1 transgenic mice [97]. EE in APPswe/PS1ΔE9 mice reduced cortical and hippocampal Aβ deposition with mice being more active in the running wheel showing an even more marked decrease in Aβ [98]. Furthermore, EE in PS1/PDAPP mice attenuated cognitive impairments [99]. Possibly in contrast with overt beneficial effects, 2month-old female APPswe/PS1ΔE9 mice exposed to EE for several months displayed increased Aβ levels in the neocortex, and hippocampus [100]. After further backcrossing these mice to a C57Bl6 background strain in order to attain fewer genetic background differences, the same group demonstrated that EE in 2-month-old transgenic APPswe/PS1ΔE9 female mice, attenuated cognitive deficits [101], but still exhibited a 25% increase in Aβ deposits in cortical and hippocampal brain regions [101]. One could argue that enhanced secretion and deposition of toxic soluble Aβ species (scavenging the toxic species into packages away   [104] from intracellular and synaptic compartments) may be a mechanistic explanation for these findings.

APOE Mice
. EE in mice carrying the APOE3 allele improved learning and memory, as assessed with the Tmaze test, while it had no effect in the ones carrying the E4 allele. The improved cognitive performance in APOE3 mice was associated with increased neocortical and hippocampal synaptophysin-and nerve growth factor-immunoreactivity, which was not observed in the APOE4 mice [102].
In conclusion, the majority of studies indicate that EE affects AD-related phenotypes in transgenic mouse models of AD pathology, mostly in a beneficial manner, particularly with regards to behavior. However, contradictory results have been reported which can possibly be explained by different experimental paradigms, age, sex, and genetic background of the mice used.

Lead.
Lead exposure has been proposed as a risk factor for AD by some authors [106,107] while others have argued against it [108]. No studies to date have performed lead exposure experiments in mouse models of AD although other animal work has indicated that lead exposure early in life may contribute to the onset of AD-related pathology later in life [109,110].

Aluminum.
While it has been proposed that occupational aluminum exposure is not a significant risk factor for AD [108], prolonged exposure to aluminum in drinking water is significantly associated with an increased risk of developing AD in a dose-dependent manner with the relative risks varying from 1.00 to 2.14 (for review see [111]). These findings should be regarded with some caution as aluminum concentrations varied highly between the different studies and many variables (such as interaction with other chemical constituents in the drinking water as well as alternative sources of aluminum, for example through antacid use or dietary intake) have often been overlooked in these studies [111].
Products made of baking-powder often contain high levels of aluminum, and it has been observed that AD patients were more frequently exposed to ingestion of foods containing baking-powder (retrospectively investigated) than age-matched controls [112]. Other studies suggested that AD patients have significantly enhanced gastrointestinal absorption of aluminum (up to 1.64 times higher) compared to age-matched controls, and indicate that differential gastrointestinal function may lead to a systemic rise of aluminum [113,114].
Praticò and colleagues [115] reported that Tg2576 mice exposed chronically to dietary aluminum displayed increased Aβ40 and Aβ42 levels, plaque deposition, and markers of oxidative stress in the hippocampus and neocortex compared to non-exposed Tg2576 mice. Others authors, however, were not able to replicate these findings. They reported that chronic aluminum treatment in Tg2576 mice did not affect Aβ load in the cerebral cortex or oxidative stress reactions in the hippocampus, nor impair spatial cognition, as measured by the MWM [115][116][117]. Aluminum treatment did raise the levels of aluminum and other metals in the hippocampus, neocortex and cerebellum, but no major differential effects could be found between Tg2576 and WT mice [116,118]. The differential effect of aluminum exposure in these mice could possibly be explained by higher concentrations of aluminum in the chow, differential ages at the start of the experiment and a shorter duration of exposure.

Iron, Zinc, and
Copper. The endogenous biometals iron, zinc and copper have often been implicated in AD, as they are present in and around amyloid deposits in the AD brain and their presence can promote aggregation of Aβ [119][120][121].
Although no report has confirmed a direct link between iron exposure and the risk of developing AD, Dwyer and colleagues do propose a ferrocentric model of AD [122]. Higher levels of ferritin iron in the basal ganglia have been considered a risk factor for AD [123,124]. AD patients show elevated levels of iron in the hippocampus [125], and this metal seems to concentrate in the core and rims of plaques in the amygdala [126].
Rodent research has indicated that gestational or early developmental iron deficiency can alter the expression of the APP and CLU genes implicated in synaptic plasticity, dendritic outgrowth, and AD pathogenesis [127][128][129]. Neonatal administration of iron for 3 days to APPswe/PS1ΔE9 mice was found not to alter Aβ deposition in the hippocampus and temporal cortex at 6 months of age but did cause changes in lipid composition, decreased steady-state levels of oxidative damage markers, and increased astrocyte levels in the temporal cortex [130].
Zinc seems to play a double role in AD etiology. Low levels of zinc have been reported to be protective against Aβ formation [131] and metalloproteases, such as neprilysin and insulin-degrading enzyme (IDE), that degrade Aβ are zinc dependent [132]. It has also been found that high levels of zinc elevate Aβ toxicity [131,133] and promote total Aβ aggregation [121]. AD patients displaying higher levels of zinc in hippocampus and amygdala [125,126] exhibited normal zinc serum levels, but significantly lower zinc levels in CSF compared to matched controls [134]. This may be explained by the binding of zinc to Aβ in the brain parenchyma [135]. However, to our knowledge, no reports have been published on putative associations between zinc exposure and risk of AD in the human population. Nonetheless, several studies have investigated the effects of altered zinc intake in AD mouse models.
Stoltenberg et al. [136] reported that lowering zinc by a 3-month dietary deficiency increased the plaque load in APPswe/PS1ΔE9 mice by 25%, without changing zinc ion distribution, zinc transporter mRNA expression levels nor inducing oxidative stress [136]. Alternatively, administrating zinc to TgCRND8 and Tg2576 mice through the drinking water for a period of 5 and 9 months, respectively, lowered the amyloid plaque burden in the hilar and molecular region of the dentate gyrus, while impairing spatial memory in MWM [137]. Concurrently, long-term administration of high zinc concentrations in TgC100 mice did not significantly affect soluble Aβ levels or levels of glial fibrillary acidic protein (GFAP), superoxidase dismutase 1, APP, βsecretase-cleaved carboxyl-terminal fragment, or neurofilament 200, a marker for neuronal damage [138]. Interestingly, a genetic reduction of zinc in the brain of Tg2576 mice, by crossing these mice with a zinc transporter 3 deficient mouse (ZnT3 −/− ), significantly reduced the plaque load in the hippocampus and neocortex while increasing the ratio between soluble versus insoluble Aβ [139].
AD patients have been shown to display reduced copper levels in the amygdala and hippocampus, while copper levels are specifically elevated in amyloid plaques [125,126]. Copper intake in AD patients decreases the reduction of Aβ42 in CSF most typically seen as the disease progresses, but does not ameliorate cognitive performance [140]. Adding copper to drinking water of cholesterol-fed rabbits causes accumulation of Aβ and the formation of plaque-like structures [141].
Exposing AD mouse models to chronic upregulation of copper has yielded conflicting results. Chronic copper administration to APP715SL mice did not alter copper, zinc, iron, Aβ nor APP levels in the brain [142]. Long-term administration of high levels of copper resulted in a 18% decrease in soluble Aβ40 and increased zinc levels in the brain without changing GFAP, SOD1, APP, C100, or NF200 levels to TgC100 mice. Yet, copper exposure in 3xTg mice led to elevated steady-state levels of APP, and C99 as well as to increased Aβ production and tau phosphorylation in the brain [143]. Interestingly, copper, APP and Aβ seem to be closely connected; Tg2576 mice displayed an overall reduction of copper in the brain whereas the ablation of APP and amyloid precursor-like protein 2 increased overall central copper levels [144,145].
In summary, both human and rodent research on the exact contributing roles of metal exposures in interaction with AD risk genes APP and PS1 remain largely inconclusive. For an overview of metal exposure in the transgenic animal models of AD listed above, see Table 4.

Human Studies of Traumatic Brain
Injury. Traumatic brain injury (TBI) has repeatedly been identified as a risk factor for AD. It has been suggested that TBI accelerates the onset of AD and that the severity of the injury increases the risk of AD [147]. AD-like pathology has been observed after acute brain trauma, even in brains of young individuals. A polymorphism in the promoter of the gene that encodes neprilysin, causing a greater length in GT repeats, has been associated with the acute development of plaques following TBI [148]. In addition, carriers of the APOE4 genotype have been associated with poorer outcome after TBI [147].

Animal Studies of Traumatic Brain Injury.
For an overview of TBI in mouse models of AD, see Table 5. After corticol contusion, 10-to 16-month-old PDAPP mice did not show significant differences in behavior or Aβ neuropathology following TBI, as compared to WT controls that underwent the same procedure of experimental brain injury [149]. Inducing TBI in the PDAPP mouse model at 4 months of age, accelerated memory loss as assessed with the MWM test. TBI also resulted in hippocampal neuronal loss one week after injury, which was associated with an increase in hippocampal Aβ40 and Aβ42 [150]. Furthermore, TBI resulted in long-term effects at 2, 5, and 8 months after TBI: a significant reduction in Aβ plaque load was found which was accompanied with more pronounced hippocampal atrophy. TBI induction in APP NLh/NLh mice caused a twofold increase in soluble hippocampal Aβ levels at 3 and 7 days after TBI. Additionally, post-TBI administration of caspase-3 inhibitors and the hypolipemic simvastatin were able to attenuate impaired hippocampal synaptic function, microglial activation and MWM performance after TBI induction in APP NLh/NLh mice [151,152]. TBI induced by cortical impact provoked gene expression changes in 22month-old APPswe mice compared to WT mice. Expression changes were detected in genes involved in various biological pathways such as immune response, cell cycle and cell death, cellular development, tissue development and connective tissue function and development, cellular movement, and hematological systems [153].
Single and repetitive mild TBI, using a cortical impact device, in 9-month-old Tg2576 mice as compared to sham treated transgenic and WT, resulted in significant cognitive dysfunction (measured with MWM) without affecting motor performance 16 weeks after TBI. However, only repetitive TBI caused increased Aβ burden in the hippocampus and neocortex with a parallel increase in isoprostane, an indicator for increased oxidative stress [154]. TBI in 10-month-old transgenic mice overexpressing either human APOE3 or 4, was associated with differential gene expression, particularly in genes related to oxidative stress, with an increased expression of antioxidant genes in the APOE3 mice as compared to the APOE4 [155].
Thus, accumulating evidence indicates that TBI interacts with AD-related genes.

Electromagnetic Field Exposure
Occupational exposure to electromagnetic field (EMF) has been proposed as a risk factor of AD. In particular, extremely low-frequency exposure has been implicated to increase the odds to develop AD up to 2.03 (as reviewed in [155]). Strikingly, Arendash et al. [27] demonstrated that longterm high-frequency exposure to EMF (i.e., similar to that generated by cell-phone use) was beneficial to AβPPswe mice (see Table 4). EMF exposure in young adult AβPPsw mice prevented the age-related genotype-specific cognitive impairment, while EMF in aged AβPPsw mice was also able to reverse cognitive impairment in these animals. Chronic EMF exposure furthermore influenced Aβ aggregation in the brain, with higher levels of soluble Aβ and less Aβ plaques in the hippocampus and entorhinal cortex of AβPPsw mice. EMF exposure has been proposed to contribute to a decrease in Aβ aggregation, via altering levels of transthyretin [158]. Transthyretin is known to sequester Aβ in CSF, thereby hindering its aggregation into amyloid plaques [158]. Interestingly, AD patients show a significant decrease in CSF transthyretin levels [159] while decreased transthyretin levels have also been found in blood serum of long-term wireless phone users [158]. Thus, effects of EMF are quite puzzling while the association with AD remains to be firmly established.

Mediterranean Diet.
Various dietary and nutritional factors seem to be protective or detrimental in the development and course of AD. One of the most prominent is the Mediterranean type of diet which has been linked to reduced risk of developing AD and showing a dose-response effect (high adherence to Mediterranean diet, OR: 0.76; moderate adherence, OR: 0.47) [160][161][162].
A typical Mediterranean diet is characterized by higher consumption of vegetables, fruits, cereals, fish, and olive    [154] oil and associated with a general higher consumption of unsaturated fatty acids and lower consumption of saturated fatty acids, usually accompanied by mild or moderate alcohol intake (preferably red wine taken with meals) [160]. The exact factors and mechanisms by which the Mediterranean diet is protective remains to be elucidated, although it has been speculated that this diet can attenuate the detrimental effects of oxidative stress and inflammation [161].

Western Diet and Obesity.
Obesity during mid-life is associated with an increased risk for AD, with an OR of 2.4, additively increasing up to 6.2 when combined with high total cholesterol levels and high systolic blood pressure [163]. Higher intake of calories and fat have been associated with increased risk for developing AD, particularly in APOE4 carriers, with a hazard ratio of 2.3 [164]. Western, highfat and low carbohydrate diet for 4 months in 1-monthold Tg2576 mice, increased levels of soluble Aβ in brain homogenates, while the treatment did not have any effect on plaque load [165]. Further, insulin resistance induced by 5 months of high-fat diet, in 9-month-old Tg2576 mice, was associated with to a twofold increase of Aβ40 and Aβ42 peptide content in the hippocampus and a twofold increase in plaque burden in the neocortex, with a concomitant acceleration of cognitive decline as measured by the MWM. In addition, γ-secretase activity was increased, while the expression of IDE was decreased by this diet [166]. APP/PS1 KI mice exposed to Western, high-fat diet showed increased oxidative stress markers as measured in brain homogenates of 2-month-old mice when compared to nontransgenic controls, but Aβ levels were not altered [167].
In another study, Western diet increased Aβ deposition in the hippocampus of the APPswe/PS1ΔE9 transgenic mice at 18 months of age, after a period of 12 months on a high-fat diet [168]. In the 3xTg mouse model of AD, a high fat diet starting at the age of 4 months for a total period of 13 months, induced similar effects in the frontal cortex [169].

Cholesterol.
As the generation, deposition, and clearance of Aβ is regulated by cholesterol, many studies have specifically focused on the implication of lipids, cholesterol metabolism, related vascular disease, APOE genotype, and their interrelationships on the development of AD [170][171][172]. The precise mechanisms underlying cholesterol and APOE4 need further investigation, as it is not clear whether cholesterol and the APOE4 genotype act as independent factors or interact with one another or whether the effect of APOE4 is partially mediated by high cholesterol levels [171][172][173][174]. Also, hypercholesterolemia in 3-monthold APP swe /PS1 M146L mice has been shown to accelerate Aβ accumulation while drug-induced hypocholesterolemia reduced the amyloid pathology [175,176].

Docosahexaenoic (DHA).
Studies in mouse models of AD amyloidosis, such as Tg2576, APPswe/PS1ΔE9, and 3xTg, have shown that a diet rich in the omega-3 fatty acid DHA reduces Aβ accumulation and somatodendritic tau accumulation, improves cognition, and induces cerebral hemodynamic changes [168,[177][178][179][180]. Such findings are in line with evidence from epidemiological studies showing a protective effect of diets rich in omega-3 fatty acids [181][182][183]. More specifically, DHA-enriched diet was shown to increase relative cerebral blood volume with a concomitant improvement in spatial memory and reduction of Aβ load in APP swe / PS1 M146L mice [184]. Exposing APPswe/PS1ΔE9 mice to a diet high in omega-3 fatty acids, however, neither improved cognition in APPswe/PS1ΔE9 mice nor reduced hippocampal Aβ, but increased omega-3 fatty acid levels in their brain [185]. Interestingly, high levels of omega-6 were linked to cognitive impairment [185].

Vitamins.
Dietary deficiency of B6, B12, and folate for 7 months increased Aβ levels in the brains of 15month-old Tg2576 mice, without altering APP, BACE-1, A disintegrin and metallopeptidase 10 (ADAM-10), nicastrin, IDE, APOE, or neprilysin [186]. Additionally, the same pattern of dietary vitamin B deficiency led to increased expression of PS1 via DNA demethylation of the promoter region of the encoding gene in brain homogenates of TgCRND8 mice [187]. In the brains of mice of the same animal model, vitamin B deficiency increased the levels of glycogen synthase kinase 3β (GSK3β) and reduced the activity of protein phosphatase 2A, which are both involved in the hyperphosphorylation of tau [188]. Furthermore, folic acid deficiency for 3 months in APPswe mice did not affect the Aβ plaque load, but induced neuron loss in the CA3 region of the hippocampus and enhanced hippocampal DNA damage, as compared to controls [189]. Besides B6 and B12, deficiency of B1, also called thiamine, exacerbated Aβ pathology via an upregulation of BACE1 in brains of Tg19959 mice [190]. Furthermore, dietary supplementation with the coenzyme Q10 for 2 months delayed hippocampal atrophy in 22month-old APP swe /PS1 Leu235Pro mice as compared to vehicle treated controls [36,37], with concurrent reduction in plaque load [36,37].
Deficiency of vitamin A has been implicated in Aβ accumulation, loss of long-term potentiation and memory impairment, while administration of its active metabolite retinoic acid for a duration of 2 months was able to rescue these deficits in the frontal cortex and hippocampus of 7month-old APPswe/PS1ΔE9 transgenic mice [191].
8.6. Caffeine and Green Tea. Besides the various nutritional factors, other lifestyle habits have also been associated with AD. Longitudinal studies have shown that coffee and tea drinking are associated with decreased risk for cognitive decline, dementia and AD in various population samples [192,193]. Another study showed a protective effect of caffeine only in women, with a relative risk of 0.49 [194], but a meta-analysis estimated an overall protective effect against dementia, with a relative risk of 0.84, though pointing out the large heterogeneity in the methods of the various epidemiological studies [195].
Acute and long-term caffeine consumption was recently shown to delay cognitive decline and lower Aβ pathology in the hippocampus of 15-month-old APPswe and APP swe / PS1 M146L mice, by suppressing βand γ-secretase levels [196,197]. Furthermore, oral or intraperitoneal administration of epigallocatechin-3 gallate, which is derived from green tea, for 2 or 6 months, exerted beneficial effects in APPswe transgenic mice, at the age of 14 months. The beneficial effects consisted of a reduction in Aβ pathology in the neocortex and hippocampus, with a parallel improvement of working memory [198][199][200]. Furthermore, administration of the citrus-derived flavonoid luteolin and its analogue diosmin for a total of 30 days, significantly reduced Aβ pathology in the hippocampus and neocortex of 9-monthold Tg2576 mice. This effect was mediated via an inhibition of GSK3β, which increased PS1 phosphorylation [201].

Wine.
Moderate red wine consumption has been shown to be beneficial. Cabernet sauvignon administration for 7 months in 4-month-old Tg2576 mice attenuated the cognitive impairment that is observed in these mice, in terms of spatial memory, when compared to ethanolconsuming and tap water Tg2576 controls. Cabernet sauvignon consumption decreased cortical and hippocampal Aβ plaque load in these mice, by promoting nonamyloidogenic processing in the direction of α-secretase cleavage [202]. Further, in vitro studies in hippocampal neuron cultures derived from Tg2576 mice, showed that the polyphenol extracts from the Cabernet sauvignon grapes increased the levels of α-secretase, which promotes the nonamyloidogenic cleavage of APP that reduced the levels of Aβ peptides [202]. Furthermore, consumption of the muscadine wine was proven to attenuate Aβ pathology in brains of 14-monthold Tg2576 mice, following a 10-month wine treatment, with a different mechanism of action. In this case, muscadine consumption reduced the aggregation of Aβ, with a parallel improvement in spatial memory [203]. The differential effect of the two types of wine was attributed to their distinct composition in polyphenolic compounds, which have a differential effect on APP processing [203].

Nicotine.
Smoking in humans has been linked with increased risk for AD [204][205][206], while nicotine administration for 6 months to transgenic mice carrying the APPswe mutation reduced the levels of insoluble Aβ in various brain regions of 15-month-old mice [207,208]. Nevertheless, nicotine administration for 5 months exacerbated hippocampal tau pathology in 6-month-old 3xTg mice, increasing tau hyperphosphorylation and aggregation, in combination with an earlier onset of these tau-related changes compared to controls [209].

Caloric Restriction.
Caloric restriction in animals has been found to prolong mean and maximum life span, reduce body fat, attenuate age-related molecular changes, and slow the decline associated with aging in various species [210]. In nonhuman primates, for example, caloric restriction prolongs lifespan and delays the onset of diseases, such as cardiovascular diseases, diabetes, and brain atrophy [211]. Restricting caloric intake by 40% for 6 weeks in APPswe and 14 weeks in APP swe / PS1 M146L mice has been shown to reduce the number and size of amyloid plaques by 40% and 55%, respectively, while also reducing the plaque-related astrocyte activation. These effects were observed in the neocortex and hippocampus [210]. Similar effects have been observed in the hippocampus of the 3xTg model after 14 months of caloric restriction [212].

Others.
In line with studies showing a beneficial effect of nonsteroidal antiinflammatory agents on AD pathology in transgenic models of AD [213], the phytogenic curcumin (a major dietary component in India) administered for 6 months reduced levels of soluble and insoluble Aβ, plaque load, oxidative stress and inflammatory response in various brain regions of 16-month-old transgenic mice carrying the APPswe mutation [214]. Additionally, blueberry supplementation and Gingko biloba extract treatment have been found to improve memory deficits in APP/PS1 and Tg2576 mice without affecting amyloid plaque load [215,216]. An overview of all nutritional and dietary factors influencing animal models of AD is given in Table 6.

Strengths, Limitations, and Future Challenges of GxE Research in AD
This paper provides an overview of experimental mouse data on environmental exposures known to be associated with AD. In general, it may be concluded that many studies have shown effects of environmental manipulations on a wide variety of phenotypes in transgenic mouse models of AD. It is challenging to evaluate the exact role of GxE in the field of preclinical AD research, due to several limitations. Numerous mouse models have been used on different genetic backgrounds and at multiple ages, applying various protocols of experimental exposures. This high level of variability makes it difficult to draw firm and general conclusions on any of the discussed exposures. In addition, the readout parameters differed per group, with some focusing on Aβ pathology, synaptic integrity, or oxidative stress and others emphasizing behavioral effects. Another limitation is that most animal models used have focused on fAD mutations, while a GxE be more applicable in sporadic forms of AD.
The experimental design of most of the available studies often consisted of testing the effects of environmental exposures only in transgenic mice, without full comparison of the effects of those environmental exposures in wild type animals. In such an experimental setup, one can merely study disease acceleration or progression as a result of environmental exposure. Additionally, some environmental factors may have a differential effect in the initiation of the disease from their effect on disease progression. Evidently, this type of research can still provide us with insights on underlying biological mechanisms, but it cannot disentangle the synergistic participation of genes and environment in the induction of an AD phenotype.
In a clinical setting, however, GxE in AD etiology is particularly complex to decipher with standard epidemiological designs, particularly because the time-window between environmental exposures during life and the clinical phenotype of AD is very long. The high variability in environmental exposure across the life span also makes it challenging to capture this interaction.
Most AD research over the past decades has been Aβcentered, yet recent clinical trials based on the amyloid cascade hypothesis have yielded controversial and sometimes disappointing results [219]. Similarly, the majority of animal models of AD have also grossly been focused on Aβenhancing mutations. Furthermore, outcome measures on the effects on environmental factors are often expressed in terms of soluble and insoluble Aβ and plaque load alterations. Because the extent of amyloid burden does not correlate well with AD symptomatology, one could argue that it would be advantageous to design future studies in a multidisciplinary manner encompassing a wide range of outcome measures such as behavioural phenotypes, biochemical, molecular, as well as neuropathological alterations and use similar outcome measures in the various studies conducted by different research groups so that results are more comparable.
Possible biological mechanisms that mediate the effects of environmental exposures and that could be the focus of further translational AD research are, among others, inflammation, oxidative stress, protein misfolding, glucose metabolism, and epigenetics [19,[220][221][222][223]. The AD brain shows ample signs of ongoing inflammatory processes, such as the presence of proinflammatory cytokines and activated microglia surrounding amyloid plaques [224]. A large body of evidence has pointed towards a role of oxidative stress and oxidative damage in brain regions that are affected by AD [220]. In addition, changes in the epigenome have been implicated in the pathophysiology of AD that can be triggered by various environmental factors [19], while the exact role of misframed proteins, such as ubiquitin+1 that have been found to accumulate in AD, remains also to be fully elucidated [223,225].   [199,200]

Concluding Remarks
It appears likely that a large part of, at least, sporadic cases can be connected with GxE, with the field being challenged to identify the most relevant GxE by for example conducting prospective clinical studies of subjects that will develop AD. Exposure to environmental risk factors during early life has been linked to other complex psychiatric phenotypes and disorders. For example, prenatal maternal and early life stress can be viewed as a risk factor for developing major depression and schizophrenia [226][227][228]. Likewise, environmental exposures during early life may well impact on the risk of developing AD. For example, neonatal exposure to metal lead has been proposed as an early environmental trigger for ADrelated pathology in rodents and macaque monkeys [109]. Findings like these have spurred researchers to formulate the "Latent Early Associated Regulation" (LEARn) theory of AD pathogenesis proposing that indeed early environmental exposures can change gene expression for long time periods and can induce pathology that only becomes apparent later in life, after subsequent trigger(s) [109,229]. While numerous challenges lie ahead, it can be argued that it is timely to move attention of epidemiological as well as experimental animal research in the field of AD towards the synergistic approach of GxE research. In a preclinical setting, one could envision focusing more on the use of recently identified genetic variants of the newly found GWAS genes. Second, current studies can benefit from the further technological advances in transgenesis that enable time-and region-specific expression of transgenes, thereby allowing for the investigation of GxE during specific time windows in development and aging, as well as in specific brain regions. Third, it may be valuable to scrutinize the roles of genetic and environmental risk factors (and their interactions) of diseases that have been associated with the onset of AD, such as cardiovascular disease and diabetes mellitus type 2. The underlying mechanisms of these disorders, which likely involve GxE, could shed a new light on the etiology of AD.
To conclude, moving towards a GxE approach in both clinical and experimental animal studies seems promising in further elucidating the multifactorial etiology of AD, and in identifying modifiable factors that are of particular relevance for subgroups of AD patients. The further use and development of animal models combining genetic and environmental manipulations will be a driving force in elucidating the exact biological underpinnings of this detrimental disorder.