Hyperphosphorylated and aggregated human protein tau constitutes a hallmark of a multitude of neurodegenerative diseases called tauopathies, exemplified by Alzheimer's disease. In spite of an enormous amount of research performed on tau biology, several crucial questions concerning the mechanisms of tau toxicity remain unanswered. In this paper we will highlight some of the processes involved in tau biology and pathology, focusing on tau phosphorylation and the interplay with oxidative stress. In addition, we will introduce the development of a human tau-expressing yeast model, and discuss some crucial results obtained in this model, highlighting its potential in the elucidation of cellular processes leading to tau toxicity.
1. Introduction
Alzheimer’s disease (AD) is the most common age-related neurodegenerative disorder, clinically manifested by a progression from episodic memory problems to a slow global decline of cognitive function that leaves patients with end-stage AD bedridden and dependent on custodial care, with death occurring on average 9 years after diagnosis [1]. The two distinctive key hallmarks of AD consist of senile plaques (SP), composed of extracellular deposits of amyloid-β peptides (Aβ), and intracellular neurofibrillary tangles (NFT), formed by accumulation of abnormal filaments of protein tau, in brain regions that serve memory and cognition [2, 3]. Although numerous studies have been performed on both Aβ and tau biology, the exact molecular mechanisms behind the pathology are still not completely elucidated. Current therapies used to treat AD patients are aimed to ameliorate symptoms for a limited period. At this point, there is no approved treatment with a proven disease-modifying effect [1, 4].
Early studies mainly focused on the Aβ biology, in part because several genetic mutations leading to early onset familial AD were identified in the genes encoding the β-amyloid precursor protein (APP) and presenilins, components of the γ-secretase complex, which are involved in the formation of Aβ peptides [5]. Over the past decade, however, tau biology has received increasing attention. In 1998, for instance, it was demonstrated that mutations in tau were responsible for another neurodegenerative disease, frontotemporal dementia and Parkinsonism linked to chromosome 17 (FTDP-17) [6–8]. These mutations unequivocally proved that tau malfunctioning, in itself, can result in neurodegeneration and cognitive decline. In addition, tau is implicated in neurodegeneration in various other diseases, such as Pick’s disease and progressive supranuclear palsy (PSP), which, similar to AD and FTDP-17, are all characterized by the appearance of intraneuronal inclusions of aggregated tau proteins [9–11]. All the diseases marked by an abnormal tau pathology are collectively annotated as “tauopathies” and strongly suggest that tau malfunction is a major factor underlying neurodegeneration in all these diseases. In this respect, many studies concerning AD have indicated an intimate link between Aβ and tau pathology, whereby Aβ accumulation would constitute an upstream event, triggering tau pathology and neurodegeneration [12, 13]. Indeed, amyloid deposition was demonstrated to precede tau tangle formation in a triple transgenic mouse model of Alzheimer's disease [14]. Furthermore, administration of Aβ42, the most fibrillogenic form of Aβ peptide, has been shown to induce the formation of tau-containing filaments, both in tissue culture [15], as in P301L transgenic mice [16]. Importantly, tau has also been shown to be essential for Aβ-induced toxicity. In cultured hippocampal neurons, tau knockout resulted in the loss of neurodegeneration in the presence of Aβ [17]. In addition, reducing endogenous tau was shown to ameliorate Aβ-induced behavioral deficits in an AD mouse model, without altering their high Aβ levels [18]. Thus, all these data indicate that, while Aβ accumulation may represent the prime trigger in the onset of AD, tau pathology likely constitutes the crucial effector of neurodegeneration in this disease. Key questions that remain to be solved involve the molecular nature of the toxic tau species as well as the molecular mechanisms leading to neurodegeneration.
2. Tau: Physiological Functions
Tau, also known as microtubule associated protein tau (MAPT), is predominantly expressed in neurons where its main function seems to be the stabilization of microtubules (MTs), particularly in axons. The MAPT gene is located on chromosome 17 and consists of 16 exons [19, 20]. In the central nervous system, alternative splicing of exons 2, 3, and 10 yields six tau isoforms (Figure 1). The isoforms differ by the presence or absence of one or two acidic inserts at the N-terminal domain, and whether they contain three or four repeats of a conserved tubulin binding motif at the C-terminal. The tubulin-binding repeat region is the central part of the microtubule-binding domain (MTBD), and tau isoforms with 4 repeats (4R-Tau) bind microtubules with greater affinity than isoforms with three repeats (3R-Tau). In normal adult human tissue, the ratio of 4R- to 3R-Tau is ~1 [21, 22]. The MT-binding affinity of tau is posttranslationally regulated primarily by serine/threonine directed phosphorylation, which can effectively modulate the binding affinity of tau for MTs [23]. Through its ability to modulate MT dynamics, tau plays a vital role in regulating the appropriate morphology of neurons. In addition, as the MT network is key to the sophisticated transport machinery allowing for transport of molecules and organelles (e.g., mitochondria and vesicles) along the axons, tau clearly can have profound effects on axonal transport and, hence, on the function and viability of neurons and their highly extended processes [24]. Under normal physiological conditions, tau is in a constant dynamic equilibrium, on and off the MTs. As MT-binding of tau is largely controlled by its phosphorylation status, cellular morphology and axonal transport are critically dependent on the balance of the activities of tau kinases and phosphatases.
Schematic representation of the six isoforms of tau, present in the central nervous system, and their amino acid lengths. The isoforms are generated by alternative splicing of exons 2, 3, and 10. As shown for the longest isoforms (2N4R), tau can be divided into the projection domain and the assembly domain, based on the cleavage by chymotrypsin after Tyr197 [25]. While tau binds to MT via the microtubule-binding domain (MTBD) in the assembly domain, sequences in the projection domain regulate, among others, the spacing between MT. In an alternative description, tau is subdivided into 4 domains: an N-terminal acidic region, followed by the proline-rich region, the MTBD, and the C-terminal tail.
Interestingly, although the primary function of tau appears to be the stabilization of microtubules, it has been shown that tau can also interact, either directly or indirectly, with actin and affect actin polymerization as well as the interaction of actin filaments with microtubules [26–31]. Furthermore, tau interacts with the plasma membrane and with several proteins involved in signal transduction, in most part via its N-terminal projection domain [32–39]. The binding of tau to signalling molecules implies that tau is either a downstream substrate or a regulator of the activity of the proteins it binds, or even both. For instance, tau is not only phosphorylated by the Fyn kinase [40, 41], it also modulates Fyn activity [42]. Interestingly, Fyn is shown to play a crucial role in the recruitment of both Aβ and tau into lipid rafts [43, 44]. The importance of these interactions of tau with proteins and structures other than the actin and microtubule cytoskeleton is largely unknown, especially in the context of tau-mediated neurodegeneration. Still, these findings support the notion that tau is prone to a large number of heterogeneous interactions, and irregularities of some of these interactions may lead, or contribute, to protein misfolding and aggregation, and even cell death through, as yet, unknown mechanisms.
3. Tau Pathology
As mentioned above, the progressive accumulation of NFT, composed of insoluble, hyperphosphorylated tau in a filamentous form, is a common hallmark of all tauopathies, including AD. As the severity of dementia in AD was shown to correlate well with NFT load, in contrast to SP load [45–47], these aggregated forms of tau were, at first, thought to be the prime toxic component. However, this concept is still under debate, as several lines of evidence indicate that the tau aggregates are not major toxic components, and may even represent a protective mechanism, by which the neuronal cell attempts to detoxify other harmful species of tau by sequestering them into relatively inert aggregates [10, 48]. Indeed, tau-mediated neuronal death, in the absence of tau filaments, is observed in Drosophila and some transgenic mouse models overexpressing human tau [49–51]. In addition, mouse models of tauopathies exist, in which a dissociation was found between tangle formation in some areas distinct from neuronal loss in others [52, 53]. Finally, in the transgenic mouse model rTg4510, conditionally expressing the human tau P301L mutant, age-related NFT develop, along with neuronal loss and memory impairment. Yet, subsequent suppression of the mutant tau was shown to stabilize neuronal loss and improve memory function, even though NFT continue to accumulate [54]. Further detailed analysis of tau pathology in this mouse model suggested that the accumulation of early-stage aggregated tau species, including hyperphosphorylated multimers of tau, and not the end-stage NFT, are correlated with the development of cognitive deficits during the pathogenic progression of tauopathy [55]. Thus, these studies suggest that tau-mediated neuronal death does not require the formation of NFT. Rather, nonfilamentous tau, in a more soluble, hyperphosphorylated, and possibly oligomeric state, may represent the prime neurotoxic tau component.
Apart from the exact nature of the toxic tau species, additional controversy surrounds the question of the molecular mechanisms mediating cell death. Regarding this issue, it is becoming increasingly evident that tau-mediated neurodegeneration may encompass multiple mechanisms, including both loss of normal functions and toxic gains-of-functions acquired by the aggregates and their precursors (Figure 2).
Chain-of-events involved in the onset and propagation of tau pathology. Several upstream events have been shown to lead to tau malfunctioning, such as Aβ-mediated effects in Alzheimer’s disease. Tau hyperphosphorylation and truncations are thought to constitute early and crucial modifications involved in tau pathology, and may mediate conformational changes leading to oligomerization and aggregation into higher-order aggregates, such as paired-helical filaments (PHF) and end-stage neurofibrillary tangles (NFT). Different forms or tau, especially hyperphosphorylated, oligomeric species, are thought to mediate toxicity via multiple mechanisms, including both loss of normal functions and gain of toxic functions. Note that some consequences of tau toxicity, such as stimulation of oxidative stress and neuroinflammation, reinforce further tau malfunctioning, creating a detrimental, self-sustaining cycle that propagates tau pathology throughout the brain. See text for details.
Tau pathology might arise from several cytoskeleton-mediated defects. Since the major physiological function ascribed to tau is the regulation of MT dynamics, tau malfunction has been reported to induce a loss of MT stability [56–58] and hamper proper axonal transport [59–63]. Impairment of these cellular functions initiates synaptic damage, an early event observed in multiple tauopathies [64–68], ultimately leading to neurodegeneration. In addition, tau may also mediate neurotoxicity, at least in part, by altering the organization and dynamics of the actin cytoskeleton [27].
Through its ability to interact with the plasma membrane and to bind a variety of proteins, tau is proposed to participate in cell signalling [32–38]. Therefore, abnormal alterations in the phosphorylation of tau, and possibly other abnormal tau modifications, may aberrantly affect its association with the plasma membrane and with various signaling molecules, possibly leading to a toxic outcome. In this respect, a model for the molecular events leading to neurodegeneration in AD was recently proposed, connecting amyloid and tau dysfunction to a Fyn-dependent, NMDA receptor-mediated excitotoxicity [69–72]. Although several aspects of this model need further confirmation, it accounts for the tau-dependency of Aβ-induced toxicity [17, 18], as well as the observed requirement of activation of NMDA receptors to induce cell death by tau overexpression in cultured neurons [69].
Aside from phosphorylation, proteolytic processing of tau constitutes another intensely studied posttranslational modification [25]. Truncation of tau may generate amyloidogenic tau fragments that initiate the aggregation of tau and/or result in tau fragments which induce neurodegeneration through unknown mechanisms, independently of tau aggregation. Caspase-mediated tau cleavage is an early event in the AD progression [73]. Although caspases are known to play essential roles in apoptosis, the involvement of the latter process in the mediation of tau-induced cell death is still obscure, as both proapoptotic and antiapoptotic features of tau are described in the literature [74–76]. Recently, in vivo imaging techniques in the transgenic rTg4510 mouse model revealed a dissociation between caspase activation and acute neuronal death in tangle-bearing neurons [77]. Therefore, these authors suggested that neurons undergo a slow, nonapoptotic but caspase-associated form of cell death in tauopathy, in which caspase cleavage of tau seeds an aggregate that actually sequesters toxic tau species. Although this process delays cell death, it results in a sick neuron that loses connections and eventually dies. Aside from the well-known involvement of caspases in tau processing, the proteasome is also shown to degrade tau [78, 79], though hyperphosphorylated tau seems resistant to this proteasome-mediated clearance [13, 80]. Interestingly, tau aggregates have been reported to inhibit proteasome activity [81], correlating with the decreased activity of the proteasome in AD-affected brain [82, 83].
As illustrated by all the studies mentioned above, tau dysfunction likely contributes to neurodegeneration via multiple mechanisms, acting at different stages of disease. Still, the exact nature of toxic tau species remains unknown, as is the sequence of events leading to tau-mediated cell death. Indeed, although correlations are quite easy to observe, it is more difficult to discern which is cause, and which is consequence. In addition, it is still unclear which aspects of aging, the greatest risk factor of all for disease development, are involved in the onset of tau pathology, and to what extent. Aging is known to affect a plethora of processes, such as glucose and insulin metabolism, inflammation, oxidative stress management, and the protein quality control system. For most of them, the molecular mechanisms connecting these processes to tau pathology are still largely elusive [25, 88–91]. The general trend, however, is that there seems to be a bidirectional relation between these processes and tau pathology, because defects in these processes seem to induce tau hyperphosphorylation and aggregation and, on the other hand, tau pathology results, for instance, in increased oxidative stress and inflammation. Thus, a picture emerges in which, when cellular stress surpasses a certain threshold level, tau toxicity is induced and a seemingly unstoppable self-sustaining cycle is created, propagating the disease throughout the brain. Further elucidating the causes of tau malfunction may provide new insights into the initiating factors of tau pathology and first toxic tau intermediates. This information will be of great value in the development of new therapeutic strategies combating tau pathologies. We will now briefly review cellular aspects involved in tau phosphorylation and oxidative stress, two important determinants of tau pathology, since, as we will discuss further below, we recapitulated elements of these features in our humanized yeast system.
3.1. Regulation of the Phosphorylation Status of Tau
The phosphorylation of tau plays a physiological role in regulating the affinity of tau for MT. In addition, tau in AD, and other tauopathies, is characterized by an abnormally hyperphosphorylated state. As the phosphorylation state of tau is controlled by the activities of various tau kinases and phosphatases, these enzymes, and their regulators, have received much attention for their role in tauopathies.
Two groups of kinases have been implicated in tau phosphorylation: proline-directed protein kinases (PDPKs) and non-PDPKs. The PDPKs include glycogen synthase kinase 3β (Gsk-3β), cyclin-dependent protein kinase 5 (Cdk5), mitogen-activated protein kinase, and several stress-activated protein kinases (SAPK). Gsk-3β and Cdk5 are the two best characterized in vivo tau kinases [92]. Both kinases copurify with MT [93–95], and phosphorylate tau within a cellular environment [96–98]. Data from in vitro studies indicate that phosphorylation of tau by Gsk-3β inhibits its ability to promote MT assembly [99, 100]. Intriguingly, Gsk-3β pseudophosphorylated mutants of tau not only displayed a decreased affinity for MT, but also reduced inducer-initiated rates of nucleation and polymerization in vitro, indicating that phosphorylation of tau by Gsk-3β might not per se lead to increased tau aggregation [101]. Multiple lines of evidence indicate that Gsk-3β is a key player affecting tau toxicity in vivo. For instance, the cotransfection of tau with Gsk-3β in a cell culture model results in more cell death compared to the expression of tau and mutant (inactive) Gsk-3β, suggesting that tau phosphorylation by Gsk-3β is toxic [102]. In addition, inhibition of Gsk3 by lithium not only reduced tau phosphorylation in vivo, but also lowered the level of aggregated tau, compared with controls [103]. Like Gsk-3β, Cdk5 is intensively investigated for its role in the development of tau pathology. Tau is a proven Cdk5 target in vivo [92] and in vitro, it was shown that phosphorylation by Cdk5 promotes tau dimerization [104]. Activation of Cdk5, by overexpressing its activator p25, accelerates tau phosphorylation and aggregation in mice overexpressing mutant P301L tau [105], and has even been shown to contribute to tau pathology in mice expressing only endogenous tau [106, 107]. Of interest, Cdk5 activity is elevated in the prefrontal cortex of AD brain, where NFT are found, but not in the cerebellar cortex, suggesting a relationship between deregulated Cdk5 activity and tau pathology in humans [108, 109]. Although Cdk5 is shown to phosphorylate tau directly [92], there are reports that Cdk5 activity also affects the tau phosphorylation status indirectly [110, 111], which, under certain conditions, may occur via a Cdk5-mediated inhibition of Gsk-3β activity [112]. Among the tau non-PDPKs are cyclic AMP-dependent protein kinase (PKA), calcium- and calmodulin-dependent protein kinase II (CaMKII), and microtubule affinity regulating kinase (MARK). MARK phosphorylates tau at KXGS motifs within the MTBD of tau, thereby inducing the release of tau from MT [113, 114]. Interestingly, in an in vitro study, it was shown that phosphorylation of tau by MARK and PKA led to a strongly reduced affinity of tau for MT, along with a decrease of tau’s ability to assemble into paired helical filaments [114]. Although at first glance this may contradict the correlation of hyperphosphorylated tau with the occurrence of NFT in tauopathies, this result is in agreement with the hypothesis that not the NFT, but soluble hyperphosphorylated forms of tau represent the toxic species as discussed above. Unbound tau, generated by MARK and/or PKA phosphorylation, may subsequently be phosphorylated by other kinases, generating the notorious “hyperphosphorylated” tau. In fact, the phosphorylation of tau by MARK may be a prerequisite for the action of downstream kinases, including Gsk-3β and Cdk5 [115].
Obviously, the phosphorylation state of tau is dictated not only by kinase activity, but also by the activities of tau phosphatases. Tau is dephosphorylated by protein phosphatases 2A (PP2A) and, to a lesser extent, by PP1, PP2B, and PP5 [37, 116–118]. In AD brain, it is found that the mRNA and protein expression levels of some of these phosphatases, as well as their activities, are decreased [118–124]. Therefore, downregulation of phosphatase activity, especially that of PP2A, can contribute to increased levels of hyperphosphorylated tau. In addition, Pin1, a member of the peptidyl-prolyl cis-trans isomerases, is involved in the regulation of the phosphorylation state of tau, as Pin1 binds tau when it is phosphorylated at Thr231 and facilitates its dephosphorylation by PP2A [39, 125–127]. Notably, Pin1 is significantly downregulated and oxidized in the AD hippocampus [128]. Furthermore, in AD neurons, Pin1 binds hyperphosphorylated tau in filaments, potentially depleting soluble Pin1 levels [39, 129].
As hyperphosphorylation of tau constitutes an early modification, inducing further conformational changes and aggregation of tau, modulation of the activities of tau kinases and phosphatases represents an appealing strategy to combat tau pathologies. Major focus has been on modulation of important tau kinases, especially Gsk-3β [103, 130], though no successful outcome has yet been reported for clinical trials using Gsk-3β inhibitors, such as lithium and valproic acid [4].
3.2. Oxidative Stress and Tau Pathology
Accumulating evidence suggests that, besides the accumulation of protein aggregates, oxidative stress and mitochondrial dysfunction, which are intimately linked, also play an important role in the etiology of neurodegenerative diseases, including AD [89, 90]. As mitochondria are a major source of reactive oxygen species (ROS), malfunctions of these organelles are thought to be a prime contributor to cellular oxidative stress. Additionally, mitochondrial dysfunction will lead to decreased energy production, which puts an extra burden on neurons, which are heavily dependent on high ATP levels to sustain many biochemical processes, especially involving neurotransmission at their synapses. Numerous reports are available demonstrating direct Aβ-mediated impairment of mitochondrial function [91]. In contrast, the link between tau and mitochondrial function is still more elusive. Still, a proteomic and functional analysis showed a mitochondrial dysfunction in P301L mice, together with reduced NADH-ubiquinone oxidoreductase activity, and, with age, impaired mitochondrial respiration and ATP synthesis [131]. In the aged transgenic mice, mitochondrial dysfunction was associated with higher levels of ROS, and increased tau pathology revealed modified lipid peroxidation levels and the upregulation of antioxidant enzymes in response to oxidative stress. Interestingly, as the P301L mice displayed an increased vulnerability towards Aβ insult, this suggests that Aβ and tau pathology work synergistically on mitochondria, through distinct mechanisms [132]. Although a direct impact of tau on some mitochondrial proteins/enzymes is hypothesized, this remains to be confirmed. In another study, the addition of annonacin, a natural mitochondrial complex I inhibitor, caused a redistribution of tau from the axons to the cell body, along with a retrograde transport of mitochondria and cell death [133]. Retrograde transport of dysfunctional mitochondria is a general feature, priming them for elimination by autophagy [134–136]. Interestingly, although annonacin addition caused an increase in oxidative stress, ATP depletion was shown to be the primary trigger for tau relocalization, mitochondrial retrograde transport, and cell death [133]. Thus, although tau pathology seems to induce mitochondrial dysfunction, leading to increased ROS, the concurrent decreased ATP levels must not be overlooked, as both these signals can play vital roles in neuronal survival.
Oxidative stress in AD and other neurodegenerative diseases has also been linked to increased brain levels of certain metals, especially iron (Fe), copper (Cu), and zinc (Zn) [137–139]. Both Fe and Cu are capable of inducing oxidative stress by stimulating free radical formation (e.g., hydroxyl radicals via Fenton reaction). In AD, Fe- and Cu-induced oxidative reactions are accelerated by Aβ, and Aβ oligomerization is stimulated in the presence of these metals. Interestingly, it was recently shown that the APP protein functions as an iron-export ferroxidase, whose activity is inhibited by Zn [140]. As Zn is locally concentrated in Aβ-plaques [141], this will lead to excessive Fe retention in APP expressing neurons, creating a self-sustaining cycle, wherein increased Fe retention induces further Aβ-oligomerization and plaque formation, further inhibiting Fe export. In regards to tau biology, metals may influence the self-assembly of tau, as low micromolar concentrations of Zn have been shown to accelerate the fibrillization of human tau via the bridging of two cysteine residues under physiological reducing conditions [142]. Under oxidizing conditions, however, intermolecular disulfide cross-linking of tau can occur, facilitating its oligomerization [143]. Furthermore, similar to Aβ plaques, NFT were found to be capable of adventitious binding of Cu and Fe in a redox-competent manner, indicating that NFT may exert prooxidant or antioxidant activities, depending on the balance among cellular reductants and oxidants in the local environment [144, 145].
Finally, oxidative stress not only results in directly damaging modifications of cell constituents, ROS also function as signalling molecules, affecting the activity of several kinases and phosphatases. Surprisingly, oxidative stress by addition of hydrogen peroxide resulted in a dephosphorylation of tau [146]. Subsequent studies indicated that, on one hand, oxidative stress induces a Pin1-mediated activation of protein phosphatase 2A [125]. On the other hand, an increased activity of protein phosphatase 1 (PP1) was observed, due to a Cdk5-mediated inhibition of inhibitor-2, a negative regulator of PP1 [111]. Thus, although Cdk5 is generally considered as a bona fide in vivo tau kinase, these and other results indicate that this kinase can influence the phosphorylation state of tau in multiple, antagonizing ways [92, 110–112]. Intriguingly, in AD brain, oxidative stress is observed, though tau is found in a hyperphosphorylated state, in contrast to the observed oxidative stress-induced dephosphorylation of tau described above. In this respect, it was found that oxidative stress combined with okadaic acid, which inhibits both PP1 and PP2A, results in a hyperphosphorylated tau species which was significantly resistant to degradation [80]. These data suggest that, in tauopathies, a combination of oxidative stress and hyperphosphorylation may be directly responsible for the accumulation of tau aggregates.
Considering the fact that oxidative stress constitutes a hallmark of numerous neurodegenerative diseases, strategies that ameliorate this stress are studied intensively as possible therapeutic treatments. Several approaches have been postulated, targeting different aspects of oxidative stress. These include the supplementation with antioxidants or a mixture of antioxidants (e.g., vitamin C and E) [138], the use of specific mitochondria-targeted antioxidants [90], and the modulation of metal bioavailability [139, 147]. Promising initial results of some of these strategies have been described, and outcomes of clinical trials are heavily anticipated.
4. A Humanized Yeast Model to Study Tau Biology
The basic cellular machinery and molecular processes between the budding yeast Saccharomyces cerevisiae and other eukaryotic species, including humans, appears to be highly conserved. Consequently, as many yeast genes have functional orthologues in mammalian organisms, yeast has been an effective model system for the study of diverse cellular processes, including mechanisms involved in glucose response [148], apoptosis [149], and cancer [150]. In addition, so-called “humanized” yeast systems have been constructed to study disease-related proteins that have no, or no apparent, functional yeast orthologue, such as the human tau protein. These humanized yeast systems have also proven to be valuable tools to unravel disease-related molecular processes and to identify novel medicinal compounds [151]. Examples of this type of studies in the field of neurodegenerative disorders include protein-misfolding disorders such as Alzheimer’s, Parkinson’s, and Huntington’s disease [152, 153].
In our laboratory, we expressed different isoforms and clinical FTDP-17 mutant forms of tau in yeast, and found that tau exhibits many of the same features as it does in neurons of patients with AD, that is, hyperphosphorylation, conformational changes, and partial accumulation into aggregates [84–86]. We will now go over our most important findings on the impact of phosphorylation as well as oxidative stress on tau properties in our yeast model.
4.1. Phosphorylation of Tau in Yeast and Its Consequences
Phosphorylation of tau in mammalian cells controls its interactions with MT while its hyperphosphorylation is thought to cause, or contribute to, the aggregation and toxicity of this protein. In order to assess tau phosphorylation in yeast, we employed a series of phosphospecific tau antibodies to scan phosphorylated residues on tau. We found that tau, when expressed in yeast, became reactive to a multitude of these antibodies, proving the existence of yeast kinases and/or phosphatases able to recognize and (de)phosphorylate human protein tau (Figure 3) [84]. In addition, we could detect tau with the conformation-dependent antibody MC1, a marker for pathological tau filaments and their precursors [154–156], and demonstrate a reproducible amount of tau present in the sarkosyl-insoluble fraction (SinT, sarkosyl-insoluble tau), pointing to tau aggregation in yeast [84].
Phosphoepitope mapping of human protein tau (2N4R isoform) in yeast. Western blotting with indicated monoclonal antibodies of total protein extracts from wild-type (lanes 1), mds1Δ (lanes 2), and pho85Δ (lanes 3) yeast strains. The arrowhead on the right denotes a slow-mobility, hyperphosphorylated tau species. Adapted from [84] with permission from the publisher and the authors.
To study the role of yeast kinases in the phosphorylation and aggregation of tau, we set out to test the involvement in tau phosphorylation of Mds1 and Pho85, functional yeast orthologues of mammalian Gsk-3β and Cdk5, respectively. Deletion of MDS1 led to a significant decrease in tau’s immunoreactivity to both AD2 and PG5. For the AD2 epitope (P-S396/P-S404), this was expected, as it constitutes a typical Gsk-3β target [50, 157, 158]. Phosphorylation of tau at S409 (PG5 epitope), however, is not a typical substrate of Gsk-3β, but of PKA, indicating that Mds1 might affect phosphorylation at this site indirectly [159, 160]. Interestingly, deletion of PHO85 resulted in a hyperphosphorylation of tau, mainly at the AD2 and PG5 epitopes [84]. This hyperphosphorylation was accompanied by an increase in MC1-reactive tau species and increased SinT levels, compared to control levels. It thus appears that Pho85 does not phosphorylate tau directly in yeast. This may not be so strange, as, also in mammalian cells, evidence exists that Cdk5 has indirect effects on the phosphorylation status in tau, exemplified by the study of Hallows et al., also demonstrating an increase in tau phosphorylation upon Cdk5 inactivation, by knockout of its activating partner p35 [110]. Since deletion of MDS1 and PHO85 both affect the AD2 and PG5 epitopes in opposite ways [84], it is tempting to speculate that Pho85 exerts its effects on tau by inhibiting Mds1. Although this has not yet been proven in yeast, we demonstrated, by means of yeast epistasis analysis combined with complementation studies using the human Gsk-3β and Cdk5 kinases, that Mds1/Gsk-3β genetically operates downstream of Pho85/Cdk5 in the phosphorylation of tau in our yeast system [86]. Intriguingly, studies in a mouse model also imply that Cdk5 might inhibit Gsk-3β activity under certain conditions [112].
Additional data on the physiological consequences of tau phosphorylation in a yeast environment were obtained via two separate in vitro tests, for which tau was purified from wild-type (WT), mds1Δ, and pho85Δ yeast strains using an anion exchange chromatography method [84, 85]. We could confirm that, after purification, tau retained its phosphorylation status, characteristic for each strain. In a first assay, we observed that the in vitro tau filament formation was much faster when using tau isolated from the pho85Δ strain, compared to tau extracted from either a WT and mds1Δ yeast strain, consistent with the hyperphosphorylated state of tau in a pho85Δ strain [84]. In addition, further fractionation of tau extracts yielded a hyperphosphorylated, MC1-reactive subfraction, and we demonstrated that this species could vastly accelerate the in vitro aggregation of tau extracted from a WT strain, implicating a seeding capacity of this hyperphosphorylated tau species. In a second series experiments, we investigated the in vitro MT binding capacity of purified tau, using taxol-stabilized MT formed with pig tubulin [85]. In this assay, we could demonstrate an inverse relation between MT binding and tau phosphorylation status, as hyperphosphorylated tau, isolated from pho85Δ cells, showed the poorest MT binding, followed by tau extracted from a WT strain, and finally tau extracted from the mds1Δ strain, which showed impaired phosphorylation.
To gain further insight into the relation between tau phosphorylation and aggregation in our yeast system, several clinical FTDP-17 mutants were expressed in WT, mds1Δ, and pho85Δ yeast strains, and their phosphorylation patterns and SinT levels were analyzed [86]. Most notably, compared to wild-type tau, both the P301L and R406W mutants displayed a clear reduction in the phosphorylation of S409 (PG5 epitope) and decreased SinT levels, particularly in a pho85Δ strain. These findings suggested that phosphorylation of tau at S409 might be an important determinant for tau aggregation. To confirm this hypothesis, we mutagenized the PG5 epitope and expressed the synthetic tau-S409A mutant and its pseudophosphorylated counterpart tau-S409E in WT and pho85Δ strains. Analysis of these synthetic mutants indeed revealed a marked decrease of tau-S409A aggregation while the tau-S409E mutant displayed SinT levels higher than, or comparable to, wild-type tau (Figure 4). Interestingly, we demonstrated that phosphorylation of S409 is also detrimental for tau-MT interaction [85], revealing a close, antagonistic link between the ability of tau to bind MT and its ability to aggregate. Intriguingly, the tau-S409A mutant was characterized with a lower AD2 reactivity while the pseudophosphorylated tau-S409E exhibited an increased immunodetection with the AD2 antibody, especially in the WT strain [86]. Thus, phosphorylation of tau at the PG5 and AD2 epitopes seems interdependent, and phosphorylation of tau at S409 might prime subsequent phosphorylation of S396/S404. These observations are in line with data from the brain of AD patients, demonstrating that the formation of the PG5 epitope on tau is an early event in the pretangle stage, and precedes the phosphorylation at S396, which is characteristic for NFT [161].
Determination of soluble (SolT) and sarkosyl-insoluble (SinT) fractions from wild-type tau or the synthetic mutants tau-S409A and tau-S409E, as obtained in WT cells (a) or pho85Δ cells. (b) Western blot with tau5 of a representative experiment is shown on the left, and quantifications of SinT levels for each experiment are shown on the right. Adapted from [86] with permission from the publisher and the authors.
In conclusion of this part, we can say that we have demonstrated that, when expressed in yeast, tau is phosphorylated at multiple pathologically relevant sites. Interestingly, Mds1 and Pho85, the yeast orthologues of human Gsk-3β and Cdk5, respectively, play crucial roles in the phosphorylation of several of these epitopes. Furthermore, our data substantiate the notion that hyperphosphorylation of tau leads to a loss of MT-binding capacity, along with the induction of conformational changes, detectable by the MC1 antibody, which ultimately lead to tau aggregation [155, 156]. Finally, we show that phosphorylation of S409 is a crucial mediator in both tau aggregation and MT binding. It is important to emphasize that the tau-MT binding assays described here were performed in vitro using MT built from pig tubulin. We are, as yet, unable to demonstrate binding of tau to yeast MT, likely due to differences in yeast and mammalian tubulins [162, 163].
4.2. In Yeast, Oxidative Stress and Mitochondrial Dysfunction Enhance Tau Aggregation Independently of Phosphorylation
As discussed previously, evidence exists suggesting a link between oxidative stress and tau pathology. We investigated the effects of oxidative stress and mitochondrial dysfunction on tau aggregation by adding Fe2+, a known inducer of oxidative stress, to yeast cells, or by examining SinT levels in specific mitochondrial mutants, respectively [86]. Interestingly, in both conditions, markedly increased tau insolubility was observed through mechanisms that are not strictly dependent on phosphorylation of tau, but rather act mainly in parallel. Close examination of Western blot profiles indicated that oxidative stress led to a reduction in the level of tau dimers, concomitant with an increase in higher-order oligomers. Furthermore, a 35 kDa degradation product appeared after the addition of Fe2+, indicative of altered processing and/or diminished clearance of tau fragments under this condition. Strikingly, application of oxidative stress led to decreased phosphorylation of tau at specific epitopes, especially AD2 and PG5 (Figure 5). This is consistent with several studies in neuronal cells showing a dephosphorylation of tau upon exposure to oxidative stress [111, 125, 146]. Two mechanisms accounting for this oxidative stress-induced tau dephosphorylation have been described, both of which seem to be conserved in yeast. In the first, increased activity of protein phosphatase 1 (PP1) was observed, due to a Cdk5-mediated inhibition of inhibitor-2, a negative regulator of PP1 [111]. Interestingly, the Cdk5/p35 complex is functionally equivalent to the yeast Pho85/Pcl6,7 complex, which phosphorylates Glc8, the orthologue of mammalian inhibitor-2, thereby controlling the activity of the Glc7 phosphatase, the orthologue of mammalian PP1 [164]. In the second mechanism, oxidative stress induced a Pin1-mediated activation of protein phosphatase 2A [125]. Pin1 function is represented by the yeast orthologue Ess1 [165], and our unpublished results indicate that disruption of Ess1 activity leads to increased hyperphosphorylation of tau. Hence, it appears that similar mechanisms may govern oxidative stress-induced tau dephosphorylation in yeast and mammalian cells, highlighting the value of studying tau biology in yeast.
Western blot analysis with the indicated antibodies, of total protein extracts isolated from tau-expressing WT and pho85Δ cells grown in a medium without (−) or with supplementation of 20 mM FeSO4 (+). See Figure 3 for antibody specificity. Adapted from [86] with permission from the publisher and the authors.
4.3. Future Perspectives for the Humanized Yeast Model
One important aspect of our research in tau-expressing yeast cells, is that we did not observe strong tau-related growth phenotypes in any of the strains, even under conditions which displayed a strong induction of tau aggregation. This implies that tau aggregation is not per se correlated with toxicity, a conclusion that correlates directly with findings in mammalian systems, in which NFT were found not to be essential for tau-induced toxicity and may even play a protective function [48, 55]. In addition, all the results discussed here concerning tau phosphorylation and aggregation, were obtained from exponentially growing yeast cells. Since, for instance, mitochondrial activity, a factor believed to be involved in the etiology of tauopathies, is more important during the yeast’s stationary phase than during fermentation, investigating the impact of tau on cellular fitness during yeast aging may present us with new clues on tau toxicity.
It is needless to say that we have just started to scratch the surface on studying possible mediators of tau biology and pathology in yeast (see Table 1). In our studies, we focused mainly on the role of Mds1 and Pho85, functional yeast orthologues of mammalian Gsk-3β and Cdk5, respectively, in the phosphorylation of tau in yeast. Obvious candidates for further research include the yeast orthologues of protein phosphatases involved in tau dephosphorylation, such as PP2A and PP1, and of Pin1. As mentioned, our unpublished results already indicate a hyperphosphorylated tau status upon decreased activity of Ess1, the yeast orthologue of Pin1. Interestingly, in contrast to a pho85Δ strain, we did observe a toxic effect of tau on growth of yeast cells with impaired Ess1 activity. This implies that the toxicity of “hyperphosphorylated” tau is not a general feature, but likely applies to a specific combination of phosphorylated epitopes. Detailed investigation of Ess1-dependent tau modifications is currently in progress. In this regard, it would be of great interest to extend this type of analysis by employing a genome-wide screen for yeast mutants displaying tau-dependent toxicity. Analysis of the phosphorylation pattern and SinT levels in such mutants can provide crucial new information regarding toxic tau species and molecular players involved in their generation.
Cellular processes involved in tau biology and/or pathology, and their amenability for study in the humanized yeast system. Readers should be aware that this table is not a complete overview of all cellular processes involved in tau biology/pathology and is meant to illustrate some opportunities and limitations of the humanized yeast system. Note the multitude of processes and orthologues, not yet studied in yeast at present.
Processes involved in tau biology/pathology
Opportunity for humanized yeast system?
Kinases/phosphatases determining the phosphorylation status of tau
(i) Multitude of phosphotau species demonstrated in yeast (see Figure 3 and [84])(ii) Yeast orthologues of important tau kinases (1) Gsk-3β (Mds1) (studied in [84–86]) (2) Cdk5 (Pho85) (studied in [84–86]) (3) PKA (Tpk1-3) (4) CaMKII (Cmk1/Cmk2)(iii) Yeast orthologues of important tau phosphatases (1) PP1 (Glc7) (2) PP2A (Pph21/Pph22, Sit4)(iv) Yeast orthologue of Pin1 (Ess1)
Tau mutations
Several clinical FTDP-17 tau mutants have been expressed in yeast, and the effects of their mutation on tau phosphorylation and aggregation have been investigated [86]
Effect of oxidative stress and/or mitochondrial dysfunction on tau pathology
Both oxidative stress and mitochondrial dysfunction are amenable to yeast studies (i) Fe2+-induced oxidative stress increases tau aggregation (SinT) in yeast [86] (ii) Tau aggregation (SinT) is increased in mitochondrial mutants sod2Δ and rim1Δ [86]
Tau binding to (i) MT (ii) Actin (iii) (plasma) membrane
(i) Binding of tau to yeast MT not (yet) demonstrated, although tau purified from yeast binds to mammalian (pig) MT in vitro [85, 86] (ii) Binding of tau to yeast actin cytoskeleton is not yet investigated (iii) Binding of tau to yeast plasma membrane or other intracellular membranes is not yet studied
Processes involved in tau clearance (i) apoptosis/caspase-cleavage of tau (ii) ubiquitin-proteasome system (iii) autophagic-lysosomal system
All these processes are present in yeast and can thus be studied for their effects on tau biology in the humanized yeast systemNote: yeast does not contain true orthologues of mammalian caspases, though it contains a caspase-related protease Yca1 (termed “meta”caspase), involved in yeast apoptosis [87]. The cleavage-specificity of caspases (cleave after aspartic acid) is different from that of metacaspases (cleave after arginine or lysine)
Aging
Stationary-phase yeast can serve as a model for aging effects. Not yet studied for effects on tau biology/pathology in the humanized yeast system
Aberrant cellular signalling
As tau has no apparent yeast orthologue, yeast cellular signalling pathways are intrinsically independent on normal tau functioning. Neuron-specific, tau-dependent signalling pathways are therefore not amenable for studies in yeast
Inflammation
Not applicable for studies in yeast. Though we note that one of the major consequences of inflammation is the generation of oxidative stress, which can be studied in a yeast environment
As a final note, we mention that, although we did not see a tau-induced toxicity in yeast, we observed synthetic toxicity upon the coexpression of tau with α-synuclein [166]. Thus, it seems that tau can exert hazardous effects in yeast, but in the absence of other stressors, this does not reveal itself by a growth defect. Increase of cellular stress, by coexpression of α-synuclein, however, apparently results in the exceeding of a certain threshold, thereby revealing a tau-dependent toxicity. Detailed analysis of impaired cellular processes upon coexpression of tau with α-synuclein, in conjunction with the above-mentioned genome-wide screening, may thus reveal which cellular machineries are affected by tau overexpression in yeast.
5. Concluding Remarks
As the group of seniors in the world’s population continues to grow, age-related neurodegenerative disorders, including tauopathies such as Alzheimer’s disease, are becoming more prevalent, and pose a serious threat to an already overwhelmed health care system. Despite the vast amount of research already performed, several aspects of tauopathies still await molecular elucidation. Among these are the nature of toxic tau species and the way they influence cell viability. We developed a yeast model expressing human protein tau variants, and demonstrated that this model recapitulates many important aspects implicated in tau pathology, including hyperphosphorylation, conformation, and aggregation. Combined with the ease of genetic manipulations, rapid genome-wide screening methods, and other advantages characteristic of yeast systems, this model may prove its value in the clarification of fundamental cellular processes involved in tau biology and pathology.
AbbreviationsAβ:
Amyloid-β peptide
AD:
Alzheimer’s disease
APP:
β-amyloid precursor protein
FTDP-17:
Frontotemporal dementia with Parkinsonism linked to chromosome 17
MT:
Microtubule
MTBD:
Microtubule-binding domain
NFT:
Neurofibrillary tangles
PDPK:
Proline-directed protein kinase
PSP:
Progressive supranuclear palsy
ROS:
Reactive oxygen species
rTg4510:
Transgenic mouse model, conditionally expressing the human tau P301L mutant
SAPK:
Stress-activated protein kinase
SinT:
Sarkosyl-insoluble tau
SP:
Senile plaques
WT:
Wild-type.
Acknowledgments
The authors would like to thank Professor F. Van Leuven (Experimental Genetics Group—LEGTEGG, KUL, Belgium), and Dr. L. Bueé and Dr. Marie-Christine Galas (INSERM U837 Alzheimer and Tauopathies, Lille, France) for collaborations and constructive discussions. This paper was supported by IWT-Vlaanderen (SBO NEURO-TARGET and Baekeland), the KULeuven Research Fund (KULeuven BOF-IOF), KULeuven R&D, and the Marie Curie Ph.D. Graduate School NEURAD.
CitronM.Alzheimer's disease: strategies for disease modification2010953873982-s2.0-7795177682910.1038/nrd2896BallatoreC.LeeV. M. Y.TrojanowskiJ. Q.Tau-mediated neurodegeneration in Alzheimer's disease and related disorders2007896636722-s2.0-3454803622710.1038/nrn2194LaFerlaF. M.GreenK. N.OddoS.Intracellular amyloid-β in Alzheimer's disease2007874995092-s2.0-3425081983910.1038/nrn2168MassoudF.GauthierS.Update on the pharmacological treatment of Alzheimer's disease20108169802-s2.0-7795020016310.2174/157015910790909520de StrooperB.Proteases and proteolysis in alzheimer disease: a multifactorial view on the disease process20109024654942-s2.0-7795106014510.1152/physrev.00023.2009HuttonM.LendonC. L.RizzuP.BakerM.FroelichS.HouldenH. H.Pickering-BrownS.ChakravertyS.IsaacsA.GroverA.HackettJ.AdamsonJ.LincolnS.DicksonD.DaviesP.PetersenR. C.StevenaM.De GraaffE.WautersE.Van BarenJ.HillebrandM.JoosseM.KwonJ. M.NowotnyP.CheL. K.NortonJ.MorrisJ. C.ReedL. A.TrojanowskiJ.BasunH.LannfeltL.NeystatM.FahnS.DarkF.TannenbergT.DoddP. R.HaywardN.KwokJ. B. J.SchofieldP. R.AndreadisA.SnowdenJ.CraufurdD.NearyD.OwenF.CostraB. A.HardyJ.GoateA.Van SwietenJ.MannD.LynchT.HeutinkP.Association of missense and 5'-splice-site mutations in tau with the inherited dementia FTDP-17199839366867027042-s2.0-003254368410.1038/31508PoorkajP.BirdT. D.WijsmanE.NemensE.GarrutoR. M.AndersonL.AndreadisA.WiederholtW. C.RaskindM.SchellenbergG. D.Tau is a candidate gene for chromosome 17 frontotemporal dementia19984368158252-s2.0-1444428410610.1002/ana.410430617SpillantiniM. G.MurrellJ. R.GoedertM.FarlowM. R.KlugA.GhettiB.Mutation in the tau gene in familial multiple system tauopathy with presenile dementia19989513773777412-s2.0-003256048710.1073/pnas.95.13.7737IngramE. M.SpillantiniM. G.Tau gene mutations: dissecting the pathogenesis of FTDP-1720028125555622-s2.0-003689230210.1016/S1471-4914(02)02440-1Spires-JonesT. L.StoothoffW. H.de CalignonA.JonesP. B.HymanB. T.Tau pathophysiology in neurodegeneration: a tangled issue20093231501592-s2.0-6164908562110.1016/j.tins.2008.11.007LudolphA. C.KassubekJ.LandwehrmeyerB. G.Tauopathies with parkinsonism: clinical spectrum, neuropathologic basis, biological markers, and treatment options20091632973092-s2.0-6004909140210.1111/j.1468-1331.2008.02513.xHardyJ.SelkoeD. J.The amyloid hypothesis of Alzheimer's disease: progress and problems on the road to therapeutics200229755803533562-s2.0-003713511110.1126/science.1072994OddoS.BillingsL.KesslakJ. P.CribbsD. H.LaFerlaF. M.Aβ immunotherapy leads to clearance of early, but not late, hyperphosphorylated tau aggregates via the proteasome20044333213322-s2.0-404316774710.1016/j.neuron.2004.07.003OddoS.CaccamoA.KitazawaM.TsengB. P.LaFerlaF. M.Amyloid deposition precedes tangle formation in a triple transgenic model of Alzheimer's disease2003248106310702-s2.0-034484513210.1016/j.neurobiolaging.2003.08.012FerrariA.HoerndliF.BaechiT.NitschR. M.GötzJ.β-amyloid induces paired helical filament-like tau filaments in tissue culture20032784140162401682-s2.0-014196003310.1074/jbc.M308243200GotzJ.ChenF.van DorpeJ.NitschR. M.Formation of neurofibrillary tangles in P301L tau transgenic mice induced by Aβ42 fibrils20012935534149114952-s2.0-003594343610.1126/science.1062097RapoportM.DawsonH. N.BinderL. I.VitekM. P.FerreiraA.Tau is essential to β-amyloid-induced neurotoxicity2002999636463692-s2.0-003719783610.1073/pnas.092136199RobersonE. D.Scearce-LevieK.PalopJ. J.YanF.ChengI. H.WuT.GersteinH.YuG. Q.MuckeL.Reducing endogenous tau ameliorates amyloid β-induced deficits in an Alzheimer's disease mouse model200731658257507542-s2.0-3424818151110.1126/science.1141736AndreadisA.BrownW. M.KosikK. S.Structure and novel exons of the human τ gene1992314310626106332-s2.0-0026488111NeveR. L.HarrisP.KosikK. S.KurnitD. M.DonlonT. A.Identification of cDNA clones for the human microtubule-associated protein tau and chromosomal localization of the genes for tau and microtubule-associated protein 2198638732712802-s2.0-0022827447GoedertM.JakesR.Expression of separate isoforms of human tau protein: correlation with the tau pattern in brain and effects on tubulin polymerization1990913422542302-s2.0-0025600995HongM.ZhukarevaV.Vogelsberg-RagagliaV.WszolekZ.ReedL.MillerB. I.GeschwindD. H.BirdT. D.McKeelD.CoateA.MorrisJ. C.WilhelmsenK. C.SchellenbergG. D.TrojanowskiJ. Q.LeeV. M. Y.Mutation-specific functional impairments in distinct tau isoforms of hereditary FTDP-1719982825395191419172-s2.0-0032484089GendronT. F.PetrucelliL.The role of tau in neurodegeneration200941, article 132-s2.0-6384917613810.1186/1750-1326-4-13RoyS.ZhangB.LeeV. M. Y.TrojanowskiJ. Q.Axonal transport defects: a common theme in neurodegenerative diseases200510915132-s2.0-1424426172510.1007/s00401-004-0952-xWangY.GargS.MandelkowE. M.MandelkowE.Proteolytic processing of tau2010384955961HeH. J.WangX. S.PanR.WangD. L.LiuM. N.HeR. Q.The proline-rich domain of tau plays a role in interactions with actin200910, article 812-s2.0-7104915807110.1186/1471-2121-10-81GalloG.Tau is actin up in Alzheimer's disease2007921331342-s2.0-3394723006510.1038/ncb0207-133FulgaT. A.Elson-SchwabI.KhuranaV.SteinhilbM. L.SpiresT. L.HymanB. T.FeanyM. B.Abnormal bundling and accumulation of F-actin mediates tau-induced neuronal degeneration in vivo2007921391482-s2.0-3394728668310.1038/ncb1528YuJ. Z.RasenickM. M.Tau associates with actin in differentiating PC12 cells2006209145214612-s2.0-3374656556710.1096/fj.05-5206comFariasG. A.MunozJ. P.GarridoJ.MaccioniR. B.Tubulin, actin, and tau protein interactions and the study of their macromolecular assemblies20028523153242-s2.0-003621946110.1002/jcb.10133ZmudaJ. F.RivasR. J.Actin disruption alters the localization of tau in the growth cones of cerebellar granule neurons200011315279728092-s2.0-0033848793Agarwal-MawalA.QureshiH. Y.CaffertyP. W.YuanZ.HanD.LinR.PaudelH. K.14-3-3 connects glycogen synthase kinase-3β to tau within a brain microtubule-associated tau phosphorylation complex20032781512722127282-s2.0-003763132410.1074/jbc.M211491200JenkinsS. M.JohnsonG. V. W.Tau complexes with phospholipase C-γ in situ19989167712-s2.0-0032484582LeeG.Todd NewmanS.GardD. L.BandH.PanchamoorthyG.Tau interacts with src-family non-receptor tyrosine kinases199811121316731772-s2.0-0344505849LiaoH.LiY.BrautiganD. L.GundersenG. G.Protein phosphatase 1 is targeted to microtubules by the microtubule- associated protein tau19982733421901219082-s2.0-003255564210.1074/jbc.273.34.21901ReynoldsC. H.GarwoodC. J.WrayS.PriceC.KellieS.PereraT.ZvelebilM.YangA.SheppardP. W.VarndellI. M.HangerD. P.AndertonB. H.Phosphorylation regulates tau interactions with Src homology 3 domains of phosphatidylinositol 3-kinase, phospholipase Cγ1, Grb2, and Src family kinases20082832618177181862-s2.0-4964911950410.1074/jbc.M709715200SontagE.Nunbhakdi-CraigV.LeeG.BloomG. S.MumbyM. C.Regulation of the phosphorylation state and microtubule-binding activity of tau by protein phosphatase 2A1996176120112072-s2.0-003046127510.1016/S0896-6273(00)80250-0VegaI. E.TraversoE. E.Ferrer-AcostaY.MatosE.ColonM.GonzalezJ.DicksonD.HuttonM.LewisJ.YenS. H.A novel calcium-binding protein is associated with tau proteins in tauopathy20081061961062-s2.0-4524909929210.1111/j.1471-4159.2008.05339.xLuP. J.WulfG.ZhouX. Z.DaviesP.LuK. P.The prolyl isomerase Pin1 restores the function of Alzheimer-associated phosphorylated tau protein199939967387847882-s2.0-003360024210.1038/21650BhaskarK.YenS. H.LeeG.Disease-related modifications in tau affect the interaction between Fyn and tau20052804235119351252-s2.0-2744443775810.1074/jbc.M505895200LeeG.ThangavelR.SharmaV. M.LiterskyJ. M.BhaskarK.FangS. M.DoL. H.AndreadisA.van HoesenG.Ksiezak-RedingH.Phosphorylation of Tau by Fyn: implications for Alzheimer's disease2004249230423122-s2.0-1214428856410.1523/JNEUROSCI.4162-03.2004SharmaV. M.LiterskyJ. M.BhaskarK.LeeG.Tau impacts on growth-factor-stimulated actin remodeling200712057487572-s2.0-3404716643910.1242/jcs.03378KawarabayashiT.ShojiM.YounkinL. H.Wen-LangL.DicksonD. W.MurakamiT.MatsubaraE.AbeK.AsheK. H.YounkinS. G.Dimeric amyloid beta protein rapidly accumulates in lipid rafts followed by apolipoprotein E and phosphorylated tau accumulation in the Tg2576 mouse model of Alzheimer's disease20042415380138092-s2.0-1114435649810.1523/JNEUROSCI.5543-03.2004WilliamsonR.UsardiA.HangerD. P.AndertonB. H.Membrane-bound β-amyloid oligomers are recruited into lipid rafts by a fyn-dependent mechanism2008225155215592-s2.0-4324911414410.1096/fj.07-9766comArriagadaP. V.GrowdonJ. H.Hedley-WhyteE. T.HymanB. T.Neurofibrillary tangles but not senile plaques parallel duration and severity of Alzheimer's disease19924236316392-s2.0-0026740795GiannakopoulosP.HerrmannF. R.BussiereT.Tangle and neuron numbers, but not amyloid load, predict cognitive status in Alzheimer's disease2003609149515002-s2.0-0038708285Gomez-IslaT.HollisterR.WestH.MuiS.GrowdonJ. H.PetersenR. C.ParisiJ. E.HymanB. T.Neuronal loss correlates with but exceeds neurofibrillary tangles in Alzheimer's disease199741117242-s2.0-034465366410.1002/ana.410410106BrettevilleA.PlanelE.Tau aggregates: toxic, inert, or protective species?20081444314362-s2.0-49149098525ProbstA.GötzJ.WiederholdK. H.Axonopathy and amyotrophy in mice transgenic for human four-repeat tau protein20009954694812-s2.0-0342803685SpittaelsK.van den HauteC.van DorpeJ.BruynseelsK.VandezandeK.LaenenI.GeertsH.MerckenM.SciotR.Van LommelA.LoosR.Van LeuvenF.Prominent axonopathy in the brain and spinal cord of transgenic mice overexpressing four-repeat human tau protein19991556215321652-s2.0-0032786370WittmannC. W.WszolekM. F.ShulmanJ. M.SalvaterraP. M.LewisJ.HuttonM.FeanyM. B.Tauopathy in Drosophila: neurodegeneration without neurofibrillary tangles200129355307117142-s2.0-0035958642AndorferC.AckerC. M.KressY.HofP. R.DuffK.DaviesP.Cell-cycle reentry and cell death in transgenic mice expressing nonmutant human tau isoforms20052522544654542-s2.0-2004436710810.1523/JNEUROSCI.4637-04.2005SpiresT. L.OrneJ. D.SantaCruzK.PitstickR.CarlsonG. A.AsheK. H.HymanB. T.Region-specific dissociation of neuronal loss and neurofibrillary pathology in a mouse model of tauopathy20061685159816072-s2.0-3364651992010.2353/ajpath.2006.050840SantacruzK.LewisJ.SpiresT.PaulsonJ.KotilinekL.IngelssonM.GuimaraesA.DeTureM.RamsdenM.McCowanE.ForsterC.YueM.OrneJ.JanusC.MariashA.KuskowskiM.HymanB.HuttonM.AsheK. H.Medicine: Tau suppression in a neurodegenerative mouse model improves memory function200530957334764812-s2.0-2234443850810.1126/science.1113694BergerZ.RoderH.HannaA.CarlsonA.RangachariV.YueM.WszolekZ.AsheK.KnightJ.DicksonD.AndorferC.RosenberryT. L.LewisJ.HuttonM.JanusC.Accumulation of pathological tau species and memory loss in a conditional model of tauopathy20072714365036622-s2.0-3414712583510.1523/JNEUROSCI.0587-07.2007AlonsoA. C.Grundke-IqbalI.IqbalK.Alzheimer's disease hyperphosphorylated tau sequesters normal tau into tangles of filaments and disassembles microtubules1996277837872-s2.0-002999978710.1038/nm0796-783AlonsoA. D. C.ZaidiT.Grundke-IqbalI.IqbalK.Role of abnormally phosphorylated tau in the breakdown of microtubules in Alzheimer disease19949112556255662-s2.0-002822796210.1073/pnas.91.12.5562AlonsoA. D. C.Grundke-IqbalI.BarraH. S.IqbalK.Abnormal phosphorylation of tau and the mechanism of Alzheimer neurofibrillary degeneration: sequestration of microtubule-associated proteins 1 and 2 and the disassembly of microtubules by the abnormal tau19979412983032-s2.0-0031012497Cuchillo-IbanezI.SeereeramA.ByersH. L.LeungK. Y.WardM. A.AndertonB. H.HangerD. P.Phosphorylation of tau regulates its axonal transport by controlling its binding to kinesin2008229318631952-s2.0-5134914358010.1096/fj.08-109181DubeyM.ChaudhuryP.KabiruH.SheaT. B.Tau inhibits anterograde axonal transport and perturbs stability in growing axonal neurites in part by displacing kinesin cargo: neurofilaments attenuate tau-mediated neurite instability200865289992-s2.0-3944913835610.1002/cm.20243IttnerL. M.KeY. D.GotzJ.Phosphorylated Tau interacts with c-Jun N-terminal kinase-interacting protein 1 (JIP1) in Alzheimer disease20092843120909209162-s2.0-6894910582110.1074/jbc.M109.014472StamerK.VogelR.ThiesE.MandelkowE.MandelkowE. M.Tau blocks traffic of organelles, neurofilaments, and APP vesicles in neurons and enhances oxidative stress20021566105110632-s2.0-003712893510.1083/jcb.200108057ThiesE.MandelkowE. M.Missorting of tau in neurons causes degeneration of synapses that can be rescued by the kinase MARK2/Par-120072711289629072-s2.0-3394730779110.1523/JNEUROSCI.4674-06.2007BigioE. H.VonoM. B.SatumtiraS.AdamsonJ.SontagE.HynanL. S.WhiteC. L.BakerM.HuttonM.Cortical synapse loss in progressive supranuclear palsy20016054034102-s2.0-0034999577BrunA.LiuX.EriksonC.Synapse loss and gliosis in the molecular layer of the cerebral cortex in Alzheimer's disease and in frontal lobe degeneration1995421711772-s2.0-002900649210.1006/neur.1995.0021DaviesC. A.MannD. M. A.SumpterP. Q.YatesP. O.A quantitative morphometric analysis of the neuronal and synaptic content of the frontal and temporal cortex in patients with Alzheimer's disease19877821511642-s2.0-0023106967LiptonA. M.Munro CullumC.SatumtiraS.SontagE.HynanL. S.WhiteC. L.BigioE. H.Contribution of asymmetric synapse loss to lateralizing clinical deficits in frontotemporal dementias2001588123312392-s2.0-0034889802TerryR. D.MasliahE.SalmonD. P.ButtersN.DeTeresaR.HillR.HansenL. A.KatzmanR.Physical basis of cognitive alterations in Alzheimer's disease: synapse loss is the major correlate of cognitive impairment19913045725802-s2.0-002598704810.1002/ana.410300410AmadoroG.CiottiM. T.CostanziM.CestariV.CalissanoP.CanuN.NMDA receptor mediates tau-induced neurotoxicity by calpain and ERK/MAPK activation20061038289228972-s2.0-3364450251610.1073/pnas.0511065103HaassC.chaass@med.uni-muenchen.deMandelkowE.mand@mpasmb.desy.deFyn-tau-amyloid: a toxic triad2010142335635810.1016/j.cell.2010.07.032HashimotoR.FujimakiK.JeongM. R.ChristL.ChuangD. M.Lithium-induced inhibition of Src tyrosine kinase in rat cerebral cortical neurons: a role in neuroprotection against N-methyl-D-aspartate receptor-mediated excitotoxicity20035381–31451482-s2.0-003743484710.1016/S0014-5793(03)00167-4IttnerL. M.littner@med.usyd.edu.auKeY. D.DelerueF.BiM.GladbachA.van EerselJ.WölfingH.ChiengB. C.ChristieM. J.NapierI. A.EckertA.StaufenbielM.HardemanE.GötzJ.jgoetz@med.usyd.edu.auDendritic function of tau mediates amyloid-β toxicity in alzheimer's disease mouse models2010142338739710.1016/j.cell.2010.06.036RissmanR. A.PoonW. W.Blurton-JonesM.OddoS.TorpR.VitekM. P.LaFerlaF. M.RohnT. T.CotmanC. W.Caspase-cleavage of tau is an early event in Alzheimer disease tangle pathology200411411211302-s2.0-324274907410.1172/JCI200420640LiuX. A.LiaoK.LiuR.WangH. H.ZhangY.ZhangQI.WangQ.LiH. L.TianQ.WangJ. Z.Tau dephosphorylation potentiates apoptosis by mechanisms involving a failed dephosphorylation/activation of Bcl-220101939539622-s2.0-7714912805310.3233/JAD-2010-1294RamalhoR. M.VianaR. J. S.CastroR. E.SteerC. J.LowW. C.RodriguesC. M. P.Apoptosis in transgenic mice expressing the P301L mutated form of human tau2008145-63093172-s2.0-4464915623310.2119/2007-00133.RamalhoWangH.-H.LiH.-L.lihonglian@mails.tjmu.edu.cnLiuR.ZhangY.LiaoK.WangQ.WangJ.-Z.LiuS.-J.cqliushijie@hotmail.comTau overexpression inhibits cell apoptosis with the mechanisms involving multiple viability-related factors201021116717910.3233/JAD-2010-091279Spires-JonesT. L.de CalignonA.MatsuiT.ZehrC.PitstickR.WuH. Y.OsetekJ. D.JonesP. B.BacskaiB. J.FeanyM. B.CarlsonG. A.AsheK. H.LewisJ.HymanB. T.In vivo imaging reveals dissociation between caspase activation and acute neuronal death in tangle-bearing neurons20082848628672-s2.0-3854912064610.1523/JNEUROSCI.3072-08.2008DavidD. C.LayfieldR.SerpellL.NarainY.GoedertM.SpillantiniM. G.Proteasomal degradation of tau protein20028311761852-s2.0-003678964710.1046/j.1471-4159.2002.01137.xGoldbaumO.OppermannM.HandschuhM.DabirD.ZhangB.FormanM. S.TrojanowskiJ. Q.LeeV. M. Y.Richter-LandsbergC.Proteasome inhibition stabilizes tau inclusions in oligodendroglial cells that occur after treatment with okadaic acid20032326887288802-s2.0-0141753062PoppekD.KeckS.ErmakG.JungT.StolzingA.UllrichO.DaviesK. J. A.GruneT.Phosphorylation inhibits turnover of the tau protein by the proteasome: influence of RCAN1 and oxidative stress200640035115202-s2.0-3384575762810.1042/BJ20060463KeckS.NitschR.GruneT.UllrichO.Proteasome inhibition by paired helical filament-tau in brains of patients with Alzheimer's disease20038511151222-s2.0-0037381710KellerJ. N.HanniK. B.MarkesberyW. R.Impaired proteasome function in Alzheimer's disease20007514364392-s2.0-003413104410.1046/j.1471-4159.2000.0750436.xSalonM. L.MorelliL.CastañoE. M.SotoE. F.PasquiniJ. M.jpasquin@qb.ffyb.uba.arDefective ubiquitination of cerebral proteins in Alzheimer's disease200062230231010.1002/1097-4547(20001015)62:2<302::AID-JNR15>3.0.CO;2-LVandebroekT.VanhelmontT.TerwelD.BorghgraefP.LemaireK.SnauwaertJ.WeraS.van LeuvenF.WinderickxJ.Identification and isolation of a hyperphosphorylated, conformationally changed intermediate of human protein tau expressed in yeast2005443411466114752-s2.0-2394446985310.1021/bi0506775VandebroekT.TerwelD.VanhelmontT.GysemansM.van HaesendonckC.EngelborghsY.WinderickxJ.van LeuvenF.Microtubule binding and clustering of human Tau-4R and Tau-P301L proteins isolated from yeast deficient in orthologues of glycogen synthase kinase-3β or cdk520062813525388253972-s2.0-3374874659610.1074/jbc.M602792200VanhelmontT.VandebroekT.de VosA.TerwelD.LemaireK.AnandhakumarJ.FranssensV.SwinnenE.van LeuvenF.WinderickxJ.joris.winderickx@bio.kuleuven.beSerine-409 phosphorylation and oxidative damage define aggregation of human protein tau in yeast2010108992100510.1111/j.1567-1364.2010.00662.xCarmona-GutierrezD.EisenbergT.ButtnerS.MeisingerC.KroemerG.MadeoF.Apoptosis in yeast: triggers, pathways, subroutines20101757637732-s2.0-7795085797210.1038/cdd.2009.219MaccioniR. B.FaríasG.MoralesI.NavarreteL.The revitalized Tau hypothesis on Alzheimer's disease20104132262312-s2.0-7795349935010.1016/j.arcmed.2010.03.007MoraisV. A.Vanessa.Morais@cme.vib-kuleuven.bede StrooperB.Mitochondria dysfunction and neurodegenerative disorders: cause or consequence201020supplement 2S255S26310.3233/JAD-2010-100345PattenD. A.GermainM.KellyM. A.SlackR. S.rslack@uottawa.caReactive oxygen species: stuck in the middle of neurodegeneration201020supplement 2S357S36710.3233/JAD-2010-100498RheinV.EckertA.Effects of Alzheimer's amyloid-beta and tau protein on mitochondrial function—role of glucose metabolism and insulin signalling200711331311412-s2.0-3514884009010.1080/13813450701572288ImahoriK.The biochemical study on the etiology of Alzheimer's disease201086154612-s2.0-7795255697010.2183/pjab.86.54IshiguroK.ShiratsuchiA.SatoS.OmoriA.AriokaM.KobayashiS.UchidaT.ImahoriK.Glycogen synthase kinase 3β is identical to tau protein kinase I generating several epitopes of paired helical filaments199332531671722-s2.0-002725581710.1016/0014-5793(93)81066-9KobayashiS.IshiguroK.OmoriA.TakamatsuM.AriokaM.ImahoriK.UchidaT.A cdc2-related kinase PSSALRE/cdk5 is homologous with the 30 kDa subunit of tau protein kinase II, a proline-directed protein kinase associated with microtubule199333521711752-s2.0-002742602910.1016/0014-5793(93)80723-8FlahertyD. B.SoriaJ. P.TomasiewiczH. G.WoodJ. G.Phosphorylation of human tau protein by microtubule-associated kinases: GSK3β and cdk5 are key participants20006234634722-s2.0-003432954310.1002/1097-4547(20001101)62:3<463::AID-JNR16>3.0.CO;2-7MichelG.MerckenM.MurayamaM.NoguchiK.IshiguroK.ImahoriK.TakashimaA.Characterization of tau phosphorylation in glycogen synthase kinase-3β and cyclin dependent kinase-5 activator (p23) transfected cells1998138021771822-s2.0-003250282910.1016/S0304-4165(97)00139-6WagnerU.UttonM.GalloJ. M.MillerC. C. J.Cellular phosphorylation of tau by GSK-3β influences tau binding to microtubules and microtubule organisation19961096153715432-s2.0-0029998294LovestoneS.HartleyC. L.PearceJ.AndertonB. H.Phosphorylation of tau by glycogen synthase kinase-3β in intact mammalian cells: the effects on the organization and stability of microtubules1996734114511572-s2.0-002999475410.1016/0306-4522(96)00126-1EvansD. B.RankK. B.BhattacharyaK.ThomsenD. R.GurneyM. E.SharmaS. K.Tau phosphorylation at serine 396 and serine 404 by human recombinant tau protein kinase II inhibits tau's ability to promote microtubule assembly20002753224977249832-s2.0-003463756010.1074/jbc.M000808200UttonM. A.VandecandelaereA.WagnerU.ReynoldsC. H.GibbG. M.MillerC. G. J.BayleyP. M.AndertonB. H.Phosphorylation of tau by glycogen synthase kinase 3β affects the ability of tau to promote microtubule self-assembly199732337417472-s2.0-0030908590SunQ.GamblinT. C.Pseudohyperphosphorylation causing AD-like changes in tau has significant effects on its polymerization20094825600260112-s2.0-6764962242710.1021/bi900602hShimuraH.SchwartzD.GygiS. P.KosikK. S.CHIP-Hsc70 complex ubiquitinates phosphorylated Tau and enhances cell survival20042796486948762-s2.0-104226662410.1074/jbc.M305838200NobleW.PlanelE.ZehrC.OlmV.MeyersonJ.SulemanF.GaynorK.WangL.LaFrancoisJ.FeinsteinB.BurnsM.KrishnamurthyP.WenYI.BhatR.LewisJ.DicksonD.DuffK.Inhibition of glycogen synthase kinase-3 by lithium correlates with reduced tauopathy and degeneration in vivo200510219699069952-s2.0-2104444922510.1073/pnas.0500466102PaudelH. K.Phosphorylation by neuronal cdc2-like protein kinase promotes dimerization of tau protein in vitro19972724528328283342-s2.0-003069282910.1074/jbc.272.45.28328NobleW.OlmV.TakataK.CaseyE.MaryO.MeyersonJ.GaynorK.LaFrancoisJ.WangL.KondoT.DaviesP.BurnsM.Veeranna.NixonR.DicksonD.MatsuokaY.AhlijanianM.LauL. F.DuffK.Cdk5 is a key factor in tau aggregation and tangle formation in vivo20033845555652-s2.0-003868916210.1016/S0896-6273(03)00259-9AhlijanianM. K.BarrezuetaN. X.WilliamsR. D.JakowskiA.KowszK. P.McCarthyS.CoskranT.CarloA.SeymourP. A.BurkhardtJ. E.NelsonR. B.McNeishJ. D.Hyperphosphorylated tau and neurofilament and cytoskeletal disruptions in mice overexpressing human p25, an activator of cdk52000976291029152-s2.0-1294426897910.1073/pnas.040577797BianF.NathR.SobocinskiG.BooherR. N.LipinskiW. J.CallahanM. J.PackA.WangK. K. W.WalkerL. C.Axonopathy, tau abnormalities, and dyskinesia, but no neurofibrillary tangles in p25-transgenic mice200244632572662-s2.0-003702974010.1002/cne.10186LeeK. Y.ClarkA. W.RosalesJ. L.ChapmanK.FungT.JohnstonR. N.Elevated neuronal Cdc2-like kinase activity in the Alzheimer disease brain199934121292-s2.0-003298874110.1016/S0168-0102(99)00026-7TsengH. C.ZhouY.ShenY.TsaiL. H.A survey of Cdk5 activator p35 and p25 levels in Alzheimer's disease brains20025231–358622-s2.0-003712520910.1016/S0014-5793(02)02934-4HallowsJ. L.ChenK.DePinhoR. A.VincentI.Decreased cyclin-dependent kinase 5 (cdk5) activity is accompanied by redistribution of cdk5 and cytoskeletal proteins and increased cytoskeletal protein phosphorylation in p35 null mice2003233310633106442-s2.0-0344011447ZambranoC. A.EganaJ. T.NunezM. T.MaccioniR. B.Gonzalez-BillaultC.Oxidative stress promotes τ dephosphorylation in neuronal cells: the roles of cdk5 and PP120043611139314022-s2.0-234246609110.1016/j.freeradbiomed.2004.03.007WenY.PlanelE.HermanM.FigueroaH. Y.WangL.LiuL.LauL.-F.WaiH. Y.DuffK. E.ked2115@columbia.eduInterplay between cyclin-dependent kinase 5 and glycogen synthase kinase 3β mediated by neuregulin signaling leads to differential effects on tau phosphorylation and amyloid precursor protein processing200828102624263210.1523/JNEUROSCI.5245-07.2008DrewesG.EbnethA.PreussU.MandelkowE. M.MandelkowE.MARK, a novel family of protein kinases that phosphorylate microtubule- associated proteins and trigger microtubule disruption19978922973082-s2.0-0030969575SchneiderA.BiernatJ.von BergenM.MandelkowE.MandelkowE. M.Phosphorylation that detaches tau protein from microtubules (Ser262, Ser214) also protects it against aggregation into Alzheimer paired helical filaments19993812354935582-s2.0-003359694610.1021/bi981874pNishimuraI.YangY.LuB.PAR-1 kinase plays an initiator role in a temporally ordered phosphorylation process that confers tau toxicity in Drosophila200411656716822-s2.0-154235889510.1016/S0092-8674(04)00170-9DrewesG.MandelkowE. M.BaumannK.GorisJ.MerlevedeW.MandelkowE.Dephosphorylation of tau protein and Alzheimer paired helical filaments by calcineurin and phosphatase-2A1993336342543210.1016/0014-5793(93)80850-TGongC. X.LidskyT.WegielJ.ZuckL.Grundke-IqbalI.IqbalK.Phosphorylation of microtubule-associated protein tau is regulated by protein phosphatase 2A in mammalian brain. Implications for neurofibrillary degeneration in Alzheimer's disease20002758553555442-s2.0-003408884610.1074/jbc.275.8.5535LiuF.Grundke-IqbalI.IqbalK.GongC. X.Contributions of protein phosphatases PP1, PP2A, PP2B and PP5 to the regulation of tau phosphorylation2005228194219502-s2.0-2764447860610.1111/j.1460-9568.2005.04391.xGongC. X.ShaikhS.WangJ. Z.ZaidiT.Grundke-IqbalI.IqbalK.Phosphatase activity toward abnormally phosphorylated τ: decrease in Alzheimer disease brain19956527327382-s2.0-0029113874LianQ.LadnerC. J.MagnusonD.LeeJ. M.Selective changes of calcineurin (protein phosphatase 2B) activity in Alzheimer's disease cerebral cortex200116711581652-s2.0-003515585410.1006/exnr.2000.7534LoringJ. F.WenX.LeeJ. M.SeilhamerJ.SomogyiR.A gene expression profile of Alzheimer's disease200120116836952-s2.0-003569247210.1089/10445490152717541SontagE.HladikC.MontgomeryL.LuangpiromA.MudrakI.OgrisE.WhiteC. L.Downregulation of protein phosphatase 2A carboxyl methylation and methyltransferase may contribute to Alzheimer disease pathogenesis20046310108010912-s2.0-5644293035SontagE.LuangpiromA.HladikC.MudrakI.OgrisE.SpecialeS.WhiteC. L.Altered expression levels of the protein phosphatase 2A ABαC enzyme are associated with Alzheimer disease pathology20046342873012-s2.0-1842510667Vogelsberg-RagagliaV.SchuckT.TrojanowskiJ. Q.LeeV. M. Y.PP2A mRNA expression is quantitatively decreased in Alzheimer's disease hippocampus200116824024122-s2.0-003507579310.1006/exnr.2001.7630GalasM. C.DourlenP.BegardS.AndoK.BlumD.HamdaneM.BueeL.The peptidylprolyl cis/trans-isomerase Pin1 modulates stress-induced dephosphorylation of Tau in neurons: implication in a pathological mechanism related to Alzheimer disease20062812819296193042-s2.0-3374581994210.1074/jbc.M601849200HamdaneM.DourlenP.BrettevilleA.Pin1 allows for differential Tau dephosphorylation in neuronal cells2006321-21551602-s2.0-3374509554210.1016/j.mcn.2006.03.006ZhouX. Z.KopsO.WernerA.Pin1-dependent prolyl isomerization regulates dephosphorylation of Cdc25C and Tau proteins2000648738832-s2.0-0033638180SultanaR.Boyd-KimballD.PoonH. F.CaiJ.PierceW. M.KleinJ. B.MarkesberyW. R.ZhouX. Z.LuK. P.ButterfieldD. A.Oxidative modification and down-regulation of Pin1 in Alzheimer's disease hippocampus: a redox proteomics analysis20062779189252-s2.0-3364651113510.1016/j.neurobiolaging.2005.05.005ThorpeJ. R.MorleyS. J.RultenS. L.Utilizing the peptidyl-prolyl cis-trans isomerase Pin1 as a probe of its phosphorylated target proteins: examples of binding to nuclear proteins in a human kidney cell line and to tau in Alzheimer's diseased brain2001491971072-s2.0-0035177565MazanetzM. P.FischerP. M.Untangling tau hyperphosphorylation in drug design for neurodegenerative diseases2007664644792-s2.0-3444750345510.1038/nrd2111DavidD. C.HauptmannS.ScherpingI.Proteomic and functional analyses reveal a mitochondrial dysfunction in P301L tau transgenic mice20052802523802238142-s2.0-2124448678110.1074/jbc.M500356200RheinV.SongX.WiesnerA.IttnerL. M.BaysangG.MeierF.OzmenL.BluethmannH.DröseS.BrandtU.SavaskanE.CzechC.GötzJ.EckertA.anne.eckert@upkbs.chAmyloid-β and tau synergistically impair the oxidative phosphorylation system in triple transgenic Alzheimer's disease mice200910647200572006210.1073/pnas.0905529106Escobar-KhondikerM.HollerhageM.MurielM. P.Annonacin, a natural mitochondrial complex I inhibitor, causes tau pathology in cultured neurons20072729782778372-s2.0-3444761951810.1523/JNEUROSCI.1644-07.2007de VosK.GoossensV.BooneE.VercammenD.VancompernolleK.VandenabeeleP.HaegemanG.FiersW.GrootenJ.johang@lmb.rug.ac.beThe 55-kDA tumor necrosis factor receptor induces clustering of mitochondria through its membrane-proximal region1998273169673968010.1074/jbc.273.16.9673LemastersJ. J.NieminenA. L.QianT.TrostL. C.ElmoreS. P.NishimuraY.CroweR. A.CascioW. E.BradhamC. A.BrennerD. A.HermanB.The mitochondrial permeability transition in cell death: a common mechanism in necrosis, apoptosis and autophagy199813661-21771962-s2.0-003250456810.1016/S0005-2728(98)00112-1MillerK. E.SheetzM. P.Axonal mitochondrial transport and potential are correlated200411713279128042-s2.0-324287555710.1242/jcs.01130HungY. H.BushA. I.ChernyR. A.Copper in the brain and Alzheimer's disease201015161762-s2.0-7204910287910.1007/s00775-009-0600-yJomovaK.kjomova@ukf.skVondrakovaD.LawsonM.ValkoM.marian.valko@stuba.skMetals, oxidative stress and neurodegenerative disorders20103451-29110410.1007/s11010-010-0563-xRivera-ManciaS.Perez-NeriI.RiosC.Tristan-LopezL.Rivera-EspinosaL.MontesS.The transition metals copper and iron in neurodegenerative diseases201018621841992-s2.0-7795354151410.1016/j.cbi.2010.04.010DuceJ. A.TsatsanisA.CaterM. A.JamesS. A.RobbE.WikheK.LeongS. L.PerezK.JohanssenT.GreenoughM. A.ChoH.-H.GalatisD.MoirR. D.MastersC. L.McLeanC.TanziR. E.CappaiR.BarnhamK. J.CiccotostoG. D.RogersJ. T.jrogers@partners.orgBushA. I.abush@mhri.edu.auIron-export ferroxidase activity of β-amyloid precursor protein is inhibited by zinc in Alzheimer's disease2010142685786710.1016/j.cell.2010.08.014DongJ.AtwoodC. S.AndersonV. E.SiedlakS. L.SmithM. A.PerryG.CareyP. R.Metal binding and oxidation of amyloid-β within isolated senile plaque cores: raman microscopic evidence20034210276827732-s2.0-034610607610.1021/bi0272151MoZ. Y.ZhuY. Z.ZhuH. L.FanJ. B.ChenJ.LiangY.Low micromolar zinc accelerates the fibrillization of human Tau via bridging of Cys-291 and Cys-32220092845034648346572-s2.0-7174911307710.1074/jbc.M109.058883SaharaN.MaedaS.MurayamaM.SuzukiT.DohmaeN.YenS. H.TakashimaA.Assembly of two distinct dimers and higher-order oligomers from full-length tau20072510302030292-s2.0-3424999008310.1111/j.1460-9568.2007.05555.xSayreL. M.PerryG.HarrisP. L. R.LiuY.SchubertK. A.SmithM. A.In situ oxidative catalysis by neurofibrillary tangles and senile plaques in Alzheimer's disease: a central role for bound transition metals20007412702792-s2.0-003396485910.1046/j.1471-4159.2000.0740270.xSmithM. A.HarrisP. L. R.SayreL. M.PerryG.Iron accumulation in Alzheimer disease is a source of redox-generated free radicals19979418986698682-s2.0-003088548210.1073/pnas.94.18.9866LoPrestiP.KonatG. W.Hydrogen peroxide induces transient dephosphorylation of tau protein in cultured rat oligodendrocytes200131121421442-s2.0-003596488310.1016/S0304-3940(01)02137-1CrouchP. J.WhiteA. R.BushA. I.The modulation of metal bio-availability as a therapeutic strategy for the treatment of Alzheimer's disease200727415377537832-s2.0-3444763414010.1111/j.1742-4658.2007.05918.xSanzP.Yeast as a model system to study glucose-mediated signalling and response200712235823712-s2.0-36049023020MadeoF.Frank.Madeo@uni-tuebingen.deEngelhardtS.HerkerE.LehmanN.MaldenerC.ProkschA.WissingS.FröhlichK.-U.Apoptosis in yeast: a new model system with applications in cell biology and medicine200241420821610.1007/s00294-002-0310-2HartwellL. H.Nobel Lecture. Yeast and cancer2002223-437339410.1023/A:1020918107706MagerW. H.WinderickxJ.Yeast as a model for medical and medicinal research20052652652732-s2.0-1804437241510.1016/j.tips.2005.03.004FranssensV.BoelenE.AnandhakumarJ.VanhelmontT.ButtnerS.WinderickxJ.Yeast unfolds the road map toward α-synuclein-induced cell death20101757467532-s2.0-7795085471710.1038/cdd.2009.203WinderickxJ.DelayC.de VosA.KlingerH.PellensK.VanhelmontT.van LeuvenF.ZabrockiP.Protein folding diseases and neurodegeneration: lessons learned from yeast200817837138113952-s2.0-4654908911110.1016/j.bbamcr.2008.01.020CarmelG.MagerE. M.BinderL. I.KuretJ.The structural basis of monoclonal antibody Alz50's selectivity for Alzheimer's disease pathology19962715132789327952-s2.0-1264426080210.1074/jbc.271.51.32789UbogaN. V.PriceJ. L.Formation of diffuse and fibrillar tangles in aging and early Alzheimer's disease20002111102-s2.0-003459448210.1016/S0197-4580(00)00091-9WeaverC. L.EspinozaM.KressY.DaviesP.Conformational change as one of the earliest alterations of tau in Alzheimer's disease20002157197272-s2.0-003428206710.1016/S0197-4580(00)00157-3NuydensR.van den KieboomG.NoltenC.VerhulstC.van OstaP.SpittaelsK.van den HauteC.de FeyterE.GeertsH.van LeuvenF.Coexpression of GSK-3β corrects phenotypic aberrations of dorsal root ganglion cells, cultured from adult transgenic mice overexpressing human protein tau20029138482-s2.0-003600855710.1006/nbdi.2001.0454SpittaelsK.van den HauteC.van DorpeJ.GeertsH.MerckenM.BruynseelsK.LasradoR.VandezandeK.LaenenI.BoonT.van LintJO.VandenheedeJ.MoecharsD.LoosR.van LeuvenF.Glycogen synthase kinase-3β phosphorylates protein tau and rescues the axonopathy in the central nervous system of human four-repeat tau transgenic mice20002755241340413492-s2.0-003473146110.1074/jbc.M006219200JichaG. A.WeaverC.LaneE.ViannaC.KressY.RockwoodJ.DaviesP.cAMP-dependent protein kinase phosphorylations on Tau in Alzheimer's disease19991917748674942-s2.0-0033198043Zheng-FischhoferQ.BiernatJ.MandelkowE. M.IllenbergerS.GodemannR.MandelkowE.Sequential phosphorylation of Tau by glycogen synthase kinase-3β and protein kinase A at Thr212 and Ser214 generates the Alzheimer-specific epitope of antibody AT100 and requires a paired-helical-filament-like conformation199825235425522-s2.0-003252159910.1046/j.1432-1327.1998.2520542.xKimuraT.OnoT.TakamatsuJ.YamamotoH.IkegamiK.KondoA.HasegawaM.YasuolhamMiyamotoE.MiyakawaT.Sequential changes of tau-site-specificphosphorylation during development of paired helical filaments199674177181KarS.FanJ.SmithM. J.GoedertM.AmosL. A.Repeat motifs of tau bind to the insides of microtubules in the absence of taxol200322170772-s2.0-003741370810.1093/emboj/cdg001GuptaM. L.BodeC. J.GeorgG. I.HimesR. H.Understanding tubulin—taxol interactions: mutations that impart Taxol binding to yeast tubulin200310011639463972-s2.0-003797566210.1073/pnas.1131967100TanY. S. H.MorcosP. A.CannonJ. F.Pho85 phosphorylates the Glc7 protein phosphatase regulator Glc8 in vivo200327811471532-s2.0-003741475410.1074/jbc.M208058200LuK. P.HanesS. D.HunterT.A human peptidyl-prolyl isomerase essential for regulation of mitosis199638065745445472-s2.0-002991612210.1038/380544a0ZabrockiP.PellensK.VanhelmontT.VandebroekT.GriffioenG.WeraS.Van LeuvenF.WinderickxJ.Characterization of α-synuclein aggregation and synergistic toxicity with protein tau in yeast20052726138614002-s2.0-1594437847510.1111/j.1742-4658.2005.04571.x