Effects of IGF-1 on IK and IK1 Channels via PI3K/Akt Signaling in Neonatal Cardiac Myocytes

Previous studies suggest that sarcolemmal potassium currents play important roles in cardiac hypertrophy. IGF-1 contributes to cardiac hypertrophy via activation of PI3K/Akt signaling. However, the relationships between IGF-1, PI3K/Akt signaling and sarcolemmal potassium currents remain unknown. Therefore, we tested the hypothesis that IGF-1 and PI3K/Akt signaling, independently, decrease sarcolemmal potassium currents in cardiac myocytes of neonatal rats. We compared the delayed outward rectifier (IK) and the inward rectifier (IK) current densities resulting from IGF-1 treatments to those resulting from simulation of PI3K/Akt signaling using adenoviral (Ad) BD110 and wild-type Akt and to those resulting from inhibition of PI3K signaling by LY294002. Ad.BD110 and Ad.Akt decreased IK and these decrements were attenuated by LY 294002. The IGF-1 treatments decreased both IK and IK1 but only the IK decrement was attenuated by LY294002. These findings demonstrate that IGF-1 may contribute to cardiac hypertrophy by PI3K/Akt signal transduction mechanisms in neonatal rat cardiomyocytes. Failure of LY294002 to effectively antagonize IGF-1 induced decrements in IK1 suggests that a signal pathway adjunct to PI3K/Akt contributes to IGF-1 protection against arrhythmogenesis in these myocytes. Our findings imply that sarcolemmal outward and inward rectifier potassium channels are substrates for IGF-1/PI3K/Akt signal transduction molecules.


Introduction
An intricate interconnected network of intracellular signaling molecules participate in regulating the electrical activity, size, and contractility of cardiac myocytes [1,2]. Dysregulation of sarcolemmal potassium currents is known to contribute to ventricular hypertrophy, arrhythmogenesis, heart failure, and sudden death in adults [3]. Insulinlike growth factor-1 (IGF-1) is an antiarrhythmogenic and antiapoptotic modulator of cardiac myocyte hypertrophy [4]. IGF-1 is thought to contribute to cardiac myocyte hypertrophy and, at the same time protect cardiac myocytes from arrhythmogenesis and apoptosis by activating the phosphatidyl inositol-3 kinase (PI3K/Akt) cell survival intracellular signaling [4,5]. However, the role of IGF-1 and PI3K/Akt signaling in mediating the sarcolemmal potassium current density remains unknown. The present study was, therefore, designed to test the hypothesis that IGF-1 and PI3K/Akt signaling independently modulate sarcolemmal I K and I K1 in cardiac myocytes.

Animal Preparation.
Conformity statement: as discussed in the following respective sections, all the procedures conform to the Guide for the Care and Use of Laboratory Animals published by the US National Institutes of Health (NIH) publication number 85-23, revised 1996. Pregnant female Sprague-Dawley rats of 200-250 g body weight were purchased from Charles Rivers (MA). The rats were allowed to recover and acquaint with their new environment upon arrival to the animal house of the Howard University College of Medicine for 3 days pre-partum. The pups were kept with the dams under secure, clean and controlled room temperature (70 • F-74 • F) with a 6:00 h to 18:00 h light cycle and the mothers were fed food and water ad libitum.

Recombinant Adenoviral Vectors.
Recombinant adenoviral vectors were prepared as previously described [6,7] by cotransfection of a shuttle vector carrying the cDNA of interest with a source of viral backbone DNA (either pAd.Track and pAd.EASY1).

Preparation of Neonatal Rat Cardiac
Myocytes. Neonatal cardiomyocytes preparation followed the Neonatal Cardiomyocyte Isolation System Kit (Worthington Biochemical Corporation). Freshly isolated cells were transferred to a water-jacketed CO 2 incubator for at least 24 h before use. Cells were placed in a perfusion bath on the stage of an inverted microscope and superfused with the appropriate recording solution at a rate of 1-2 mL/min. Isolated cultured cells were incubated with IGF-1 or the LY294002 inhibitor of PI3K signaling, with normal culture media serving as the control. Cells were also transfected with adenovirus carrying the constitutive BD110 activator of PI3K or carrying the wild-type Akt mutant before use, with adenoviral enhanced green fluorescent protein (Ad.EGFP) serving as the control. K1 . Ventricular myocytes were placed in a culture dish with an extracellular buffer containing (mM): 5 KCl, 1 MgCl2, 140 NaCl, 10 HEPES, 10 D-glucose, 1 CaCl2, 0.2 CdCl2, to which we added 0.1 μM IGF-1 [8] and/or 3 μM LY 294002 [8], Ad.Akt 10 11 pfu/mL (10,11] and/or Ad.BD110 10 11 pfu/mL [6,7], and pH at 7.4. The voltage dependency of I K activation was studied by obtaining data for the respective current-voltage (I-V ) relationships. To that end, 350 ms step voltages in 10 mV increments between −40 mV and +30 mV were applied from a holding potential of −80 mV, but with a 200 ms prepulse to −40 mV. For I K1 , 350 ms step voltages in 10 mV increments between −140 mV and −90 mV were applied from a holding potential of −80 mV. Steady-state currents, measured at the end of each current response, were plotted as a function of the command potential. The actions of IGF-1, in the presence and absence of the constitutive Ad. BD110 or wild-type Ad.Akt activators and in the presence and absence of the LY294002 inhibitor of PI3K signaling, were analyzed for their effects on the current-voltage (I-V ) relationship. LY 294002, an inhibitor of PI3K, was purchased from Cell Signaling Technology (MA). Sarcolemmal potassium currents (I K and I K1 ) were expressed in current density, pA/pF. Ad.EGFP data served as the control for evaluating the effects of transfections.

Statistical
Analysis. The effects of treatments of neonatal cardiomyocytes with IGF-1 alone and in combination with the constitutive Ad.BD110 or wild-type Ad.Akt active vectors or with the LY294002 inhibitor of PI3K signaling on the pA/pF values at each membrane potential were compared by repeated measures ANOVA with significance set at P < 0.05.

Effects of IGF-1 on I K1 .
To determine whether the electrophysiological activity of I K1 is regulated by IGF-1/PI3K/Akt pathway, the cardiomyocytes were treated with IGF-1 or transfected with Ad.Akt. Figure 5 shows that I K1 current density was decreased after treatments with IGF-1 by 23.46 ± 3.8% (−7.5 ± 0.9 pA/pF; n = 8, P < 0.05) or, after transfection with Ad.Akt, by 38.54 ± 1.8% (−5.9 ± 0.9 pA/pF; n = 6, P < 0.05), compared to the normal control (−9.8 ± 0.9 pA/pF; n = 10) and to the Ad.EGFP control values (−9.7 ± 0.8 pA/pF; n = 8). Recordings representative of the current responses are shown in Figure 6. Figure 7 shows that application of the PI3K inhibitor LY294002 failed to alter both the IGF-1 and the Ad.Akt effects on I K1 density.
Recordings representative of the current responses for the LY294002 combination treatments are shown in Figure 8.

Discussion
During ventricular hypertrophy, changes in ion channel activity are mediated by intracellular signal transduction mechanisms connected to the addition or subtraction of the fetal gene program [9]. IGF-1 is known to activate PI3K and its downstream target Akt, a serine threonine kinase, also known as protein kinase [10][11][12]. IGF-1 binding to its receptor with downstream activation of PI3K and Akt has been demonstrated [13,14]. IGF-1 is known to play a major role in cellular proliferation and differentiation [15][16][17]. Treatment of cultured newborn rat cardiac myocytes with IGF-1 is reported to activate specific genes that accompany electrophysiological changes [18] and IGF-1 mRNA and protein to increase in parallel with alterations in sarcolemmal ion currents [19]. Furthermore, overexpression of Akt appears to induce changes in the electrophysiological properties of the hearts of transgenic mice [20,21]. These findings suggest important roles for IGF-1, as well as significant gaps in knowledge concerning IGF-1 regulation of sarcolemmal potassium channels by the PI3K/Akt cell survival signal transduction pathway.
In the present study, we characterized the alterations of potassium channel current density (I K and I K1 ) associated with treatments of cultured neonatal rat cardiomyocytes with IGF-1 alone and in combination with two activators (Ad.BD110, Ad.Akt) and one inhibitor of the PI3K/Akt signaling pathway. We demonstrated significant decrements in I K and I K1 current density by IGF-1. The effects of IGF-1 on I K seemed to be mediated, largely, by PI3K/Akt signaling. Although I K1 was found to be sensitive to treatment with IGF-1 and Ad.Akt, LY294002 had no significant effect on the IGF-1-induced decrement in I K1 density. This finding suggests that IGF-1 might modulate I K1 by another mechanism cross-talking downstream from PI3K/Akt signaling and that the inward rectifier channel (Kir) may not be a direct substrate for the PI3K/Akt signaling molecules.
The stimulators of intracellular signal transduction molecules Ad.BD110, a constitutively active PI3K and Ad.Akt, wild-type Akt, decreased I K density. The wild-type Ad.Akt also decreased I K1 density. The Akt simulator Ad.Akt also decreased I K1 density. However, the PI3K inhibitor LY294002 effectively antagonized the effects of IGF-1, Ad.BD110, and Ad.Akt on I K density but failed to antagonize the effects of IGF-1 and Ad.Akt on I K1 density. A limitation of this study is that the effects of Ad.BD110 on I K1 density were not determined. Nevertheless, these results imply that both PI3K and Akt signaling pathway molecules play roles in the decrement in I K density associated with IGF-1, an effect known to increase the duration of cardiac myocyte action potentials. However, although Akt signaling seems to play a role in the decrement in I K1 density associated with IGF-1, this effect appears to be independent of PI3K activity.
The IGF-1/PI3K/Akt signaling pathway is the best characterized example of intracellular signal transduction contributing to physiological and pathological cardiac hypertrophies. IGF-1/PI3K/Akt signaling molecules are reported to be critical for exercise-induced cardiac hypertrophy [5,13], in concert with AMP-activated kinase [22]. The effects of IGF-1/PI3K/Akt signaling on I K and I K1 in exercise-hypertrophied cardiomyocytes have not been demonstrated. It is tempting to speculate that IGF-1/PI3K/Akt signaling mediates physiological growth and hypertrophy and antagonizes pathological hypertrophy [13]. However, the mechanisms underlying pathological hypertrophy appear to be more complex. We have demonstrated that IGF-1/PI3K/Akt signaling also plays a role in eccentrically hypertrophied adult rat cardiomyocytes, decreasing the current densities of both I K and I K1 [8,23]. IGF-1 appears to upregulate the transient outward potassium current associated with the early repolarization of phase 1 of the ventricular myocyte action potential in normal neonatal rat cardiomyocytes [24]. Although Ito initiates repolarization, the delay in completing repolarization and prolonging depolarization is mainly a function of the phase 3 outwardly rectifying current, I K . We previously reported that downregulation of ATP-sensitive potassium channel current (I KATP ) is a characteristic of volume-overloaded, eccentrically hypertrophied adult rat cardiac myocytes which is thought to increase the duration of ventricular action potentials and increase inotropy of the myocytes [25]. The IGF-1 induced decrement in I K described in the present study might also contribute to prolonging ventricular action potentials. This IGF-1 induced decrement in I K was simulated by activating the PI3K/Akt pathway using both Ad.BD110 and Ad.Akt and has been shown to be sensitive to inhibition by the PI3K inhibitor LY294002. These findings suggest that PI3K/Akt signaling molecules could serve as substrates for inhibiting cardiomyocyte hypertrophy in neonatal cardiac myocytes. I K1 is the inwardly rectifying potassium current responsible for restoring the resting membrane potential and excitability International Journal of Cell Biology 5 of ventricular myocytes. Upregulation of I K1 is reported to be a significant contributor to the cardiac arrhythmias associated with hypertrophy of cardiac myocytes [26,27]. The IGF-1-induced decrement in I K1 , simulated by activating the PI3K/Akt signaling pathway using Ad.Akt and insensitive to the PI3K inhibitor LY294002, is, therefore, likely to be dependent on the signaling molecules associated with an alternate or adjunctive signaling pathway such as AMPactivated kinase shown to play a role in cardiac myocyte hypertrophy [22]. The IGF-1-induced decrement in I K1 , by virtue of the role of I K1 on resting membrane excitability, is likely to protect against the arrhythmogenesis associated with cardiac myocyte hypertrophy [5]. Decreasing the inward potassium resting potential stabilization current may, therefore, increase the threshold for ventricular arrhythmogenesis, and the Kir channels in neonatal cardiac myocytes might, therefore, function as substrates for treatments to inhibit the predictable, potentially fatal ventricular arrhythmias associated with adult cardiac hypertrophy [28][29][30][31] and with hypertrophy associated with neonatal cardiomyopathy [32].
We did not look for or observe any significant changes in the size of the neonatal cardiac myocytes that we studied. These normal neonatal cardiac myocytes were used as a model to determine whether the decrements in I K and I K1 density, which we previously observed in eccentrically hypertrophied adult rat cardiac myocytes and not in normal adult rat cardiac myocytes exposed to IGF-1 [8,23] were effects inherent or specific to such hypertrophied cardiac myocytes. The results of the present study, demonstrating decreased I K and I K1 density in normal neonatal cardiac myocytes, suggest that similar effects could be elicited from not only eccentrically hypertophied adult rat cardiomyocytes, but also from normal neonatal rat cardiomyocytes treated with, or exposed to, IGF-1.

Conclusions
The present study demonstrates that stimulation of the IGF-1/PI3K/Akt cell survival signaling pathway decreases activities of the sarcolemmal I K outward and the I K1 inward rectifier currents of neonatal cardiac myocytes. These results in normal neonatal rat cardiac myocytes are in agreement with our previous reports in volume-overloaded, eccentrically hypertrophied adult rat cardiac myocytes. Our findings imply that sarcolemmal outward and inward rectifier potassium channels are substrates for IGF-1/PI3K/Akt signal transduction molecules. Comparisons of the effects of cellular signal transduction molecules on normal and hypertrophied cardiomyocytes may be useful experimental models for elucidating the sarcolemmal ion channel substrates and regulatory mechanisms underlying both neonatal and adult cardiac cardiomyopathies.