Electrochemical Analysis of Ultrathin Polythiolsiloxane Films for Surface Biomodification

The ability of different crosslinkers to crosslink nanometer thick films of the polymer poly(mercaptopropyl)methylsiloxane (PMPMS), thus stabilizing these films on solid supports, was investigated. The four crosslinkers included 1,11-bismaleimidotriethyleneglycol (BM(PEG)3), tris-(2-maleimidoethyl)amine (TMEA), bismaleimidohexane (BMH), and 1,1 -(methylenedi-4,1phenylene) bismaleimide (BMDPM). PMPMS films treated with the four crosslinkers were compared in the effectiveness of achieved crosslinking, continuity and stability of the films to rearrangement at elevated temperatures, andmodification with singlestranded DNA. The results of electrochemical analyses show that more hydrophilic crosslinkers had difficulty reacting fully with PMPMS thiols, even in these nanometer thin layers. This observation highlights the critical importance of selecting crosslinkers that are chemically compatible. Optimal selection of crosslinker yielded films in which the polymer film was largely incapable of rearranging, even at elevated temperatures, yielding reproducible and stable layers. These results validate use of these supports for applications such as monitoring thermal denaturation of immobilized DNA duplexes.


Introduction
Thermal stability of biomolecular films on solid supports is key to their diagnostic and related applications which typically require elevated temperatures for optimal performance, as well as to basic research on such layers.For example, thermal denaturation has been used to analyze thermodynamics of hybridization on solid supports including effects of chain length [1][2][3], probe associations [4,5] and surface density [6], probe-surface interactions [7,8], salt concentration [9][10][11][12][13][14], and how such systems perform in commercial technologies [15].Similar needs arise for immobilized protein films such as antibodies or enzymes [16][17][18], where the impact of the surface environment on stability or activity of the protein could be correlated with determination of its denaturation transition and unfolding.Such applications require immobilization chemistries capable of performing at temperatures approaching physical limits, like the boiling point of water.For instance, studies of DNA monolayers that require determination of melting curves expose such films for hours to temperatures of up to 80 ∘ C [14].
On glass type supports, organosilane chemistry provides stable immobilization [14].Other common supports include conductive materials such as gold, which are especially popular in academic research but are also encountered in commercial systems [19].Gold provides convenient immobilization via thiol moieties, tailorable surface chemistry, stability under aqueous buffers, and convenient interface for electrochemical characterization.Often, biomolecules are immobilized to gold using a single thiolate bond; this approach, however, is susceptible to degradation by time and temperature.Approaches based on multivalent attachment through several thiolate bonds have produced significantly more stable immobilization [20][21][22][23][24]. Fragoso and O'Sullivan also reported a markedly more robust attachment based on reduction of individual diazonium moieties and showed it to be significantly more stable than even dithiol attachment [25].Another class of methods utilize polymeric base layers that are a few nanometers thick and adhere irreversibly to gold, to which the biomolecules are conjugated through stable covalent bonds [26].One such very effective approach is to use polythiolsiloxane base films in which the polymers are bound to the gold support through multiple thiolate bonds, with remnant thiols serving for attachment of the biomolecules, typically through thioether linkages [27,28].
Due to the typical application of these systems in bioelectronic sensors, their electrochemical characterization is of great interest.In this paper, electrochemical methods are applied to evaluate stability-related properties of polythiolsiloxane films as a function of crosslinker selection, where the crosslinker serves to simultaneously bind the base layer chains to each other as well as to activate the layer for attachment of biomolecules.Four commercially available, maleimide-based crosslinkers are evaluated for their ability to realize high degrees of crosslinking of nanometer thick layers of the polythiolsiloxane polymer poly(mercaptopropyl)methylsiloxane (PMPMS), with the further goal to maximize tolerance to high temperatures.Realization of optimal stability requires crosslinkers to partition into the polymer layer so as to gain access to its thiol groups, a consideration that turns out to be crucial even for these ultrathin films.The impact of crosslinking on hydrophobicity and other properties of the crosslinked support are also considered.The crosslinker most effective for creating stable layers is used to demonstrate measurement of melting transitions in immobilized DNA films [4].

Preparation of PMPMS Layers.
The preparation of gold disk working electrodes was described previously [4].
Electrodes were polished manually and electrochemically, followed by measurement of their roughness factor  from differential capacitance under 1 mM NaF [29,30].Next, the cleaned electrodes were washed with deionized water, rinsed with dimethyl formamide (DMF), and transferred, while still wet, into 10 mM solutions (by monomer residue) of PMPMS in DMF while stirring for 2 hrs.After chemisorption of PMPMS, electrodes were rinsed extensively with DMF to remove unattached PMPMS.The PMPMS-coated and washed electrodes were next immersed in 1 mM solutions of one of the four crosslinkers in DMF, while stirring, for durations of up to 17 hrs, followed by thorough rinsing with DMF and drying under compressed nitrogen.This step crosslinks the separate PMPMS chains into an infinite network, provided crosslinkers react so as to span between thiols from different PMPMS chains.Maleimides that are left unreacted can be used for subsequent immobilization of thiol-bearing biomolecules.

Preparation of DNA Monolayers
. PMPMS films, crosslinked and maleimide-activated as described above, were immediately placed in 1 M solutions of thiol-terminated DNA probes in SPB at room temperature for 1 hour.Thiolterminated probes were prepared by cleaving the 5  disulfide with dithiothreitol, followed by purification and resuspension in SPB as previously described [4].Control experiments used the as-received, disulfide-protected probe DNA.After probe immobilization, electrodes were rinsed with deionized water and SPB.

Measurement of PMPMS Thickness.
The thickness of PMPMS films was estimated from the differential capacitance per area,   , measured with electrochemical impedance spectroscopy (EIS).Previously, using PMPMS films prepared on gold-coated slide supports, this approach was shown to yield thickness values in good agreement with ellipsometric measurements [28].EIS measurements used 3 mV (rms) amplitude AC modulation swept from 10 Hz to 10 kHz, on top of a DC offset of 0 mV versus an Ag/AgCl/3 M NaCl reference.A Pt wire counterelectrode was used on a CH Instruments workstation.The experimental impedance  was interpreted in terms of an equivalent circuit composed of a solution resistance   in series with a constant phase element (CPE) representing the charging response of the electrode.A CPE was used instead of a pure capacitor to allow for dispersion in the capacitive charging caused by roughness or chemical heterogeneity of the interface [31,32].The impedance of a CPE is given by where  is the frequency,  (0 ≤  ≤ 1) and  are parameters characterizing the CPE and  is the square root of -1.An  of 1 corresponds to an ideal capacitor, while  = 0 would be a resistor with resistance 1/.An effective capacitance per area,   , is calculated from [32]   = (  1− )  where  is the actual electrode area given by the product of the geometric area and the roughness factor .   , , and  were determined by fitting of the experimental frequencydependent impedance to a model consisting of a CPE element in series with the solution resistance;   was then calculated from (2).Fitted values of  for neat PMPMS films were 0.98 or higher, indicating that the PMPMS films exhibited close to ideal capacitor behaviour.Based on this observation, the PMPMS thickness was calculated from the parallel plate capacitor result where  = 6.0 is the dielectric constant of PMPMS [33],   is the permittivity of free space, and  is the PMPMS layer thickness.To minimize contribution of the diffuse double layer to   and hence , EIS measurements were performed in a high ionic strength, 1 M SPB, buffer.Under these conditions   is dominated by capacitance of the PMPMS layer and provides thickness values in good agreement with ellipsometric measurements [28].
Figure 2 shows examples of EIS data in the form of a Nyquist plot of the measured imaginary   and real   impedance components, as a function of crosslinking time for PMPMS layers crosslinked with BMDPM.Addition of crosslinker caused a clear change from the uncrosslinked layer at 0 min.Fits for the 0 and 30 min datasets are also plotted (solid lines).For the assumed model of a series   and CPE circuit, the experimental impedances are fit using   =   + cos(/2)/(  ) and   = −sin(/2)/(  ) by varying   , , and .The effective capacitance   then follows from (2).

Measurements of PMPMS Continuity.
The physical continuity of PMPMS layers was investigated by monitoring electron transfer across the layer from ferri/ferrocyanide [Fe(CN) 6 ] 3−/4− anions in solution.Continuous films prevent the ions from approaching the gold surface, diminishing their redox activity [34].These experiments used test solutions of 10 mM potassium ferri/ferrocyanide [Fe(CN) 6 ] 3−/4− redox couple in 200 mM KCl.An AgCl coated silver wire served as a quasi-reference electrode, with a Pt wire counterelectrode.Cyclic voltammograms (CVs) were measured from -300 to 600 mV with a scan rate of 20 V/s.The redox potential of the Fe(CN) 6 3−/4− redox couple was observed at 250-300 mV.All measurements were initiated with fresh test solutions to minimize possible degradation of the redox couple.
International Journal of Electrochemistry 2.6.Melting Curve Measurements.Melting curve measurements on immobilized duplexes were performed as in prior work [4].In brief, target oligonucleotides were labeled with ferrocene FcCA-NHS tags, followed by purification using reversed-phase HPLC.The FcCA-labeled targets were hybridized to PMPMS-anchored DNA monolayers using 25 nM solutions in 0.5 M SPB and the extent of hybridization was tracked as a function of temperature.Hybridization measurements were performed under a nitrogen purge to minimize dissolved oxygen, which contributes to ferrocene degradation via the oxidized ferricinium state [35].A temperature controlled, jacketed electrochemical cell (Gamry Instrument, Warminster, PA) was used.Experiments started at a high temperature of 75 ∘ C, were cooled at 0.3 degrees/min to 30 ∘ C, and then heated back to 75 ∘ C. CV curves were measured as a function of temperature during both the cooling and heating cycles, and the extent of hybridization was determined by integrating the ferrocene faradaic peak as described below.
All CV measurements used a potential sweep rate of 20 V s −1 , applied between 0 and 700 mV to capture the ferrocene peak.

Calculation of DNA Surface
Coverage.DNA coverage, in molecules/area, was derived from the charge  needed to switch the FcCA tags' oxidation state. was determined from cyclic voltammetry traces (range: 0 to 700 mV, scan rate: 20 V/s) by integrating the area of the ferrocene tag oxidation peak after correction for baseline current and converting the potential axis to time [36].Since each strand has just one label, the corresponding molecular coverage, , can be calculated using Here,  = 1.60 × 10 −19 , C is the elementary charge,   = 0.02 cm 2 is the geometric electrode area, and  is the surface roughness factor.

Results and Discussion
The extent of crosslinking was expected to be a primary parameter governing stability of the base polymer layer; moreover, by deactivating PMPMS thiols it serves to prevent their subsequent participation in immobilization of thiolcarrying biomolecules through alternate pathways such as formation of disulfide bridges [37].Following this reasoning, crosslinkers were first ranked according to their ability to reach high extents of conversion of PMPMS thiols.The most promising crosslinkers were then investigated further with respect to optimization of stability and electrochemical properties of the films.

Crosslinker Effectiveness.
The four crosslinkers were evaluated for their ability to maximize crosslinking.Crosslinked PMPMS films, prepared as described earlier, were exposed to solutions of disulfide-terminated probes.While the disulfide groups would be inert toward any unreacted crosslinker maleimides in the films, they could undergo exchange reactions with remnant PMPMS thiols.In the desired case all PMPMS thiols would be already consumed by crosslinker, so that there should be no immobilization of the probes.The assays were performed by immersing crosslinked PMPMS films in 1 nM solutions of 5  disulfide-terminated probes in 1 M, pH 7.0 SPB for 1 hr.Subsequent to probe immobilization, the layers were hybridized with complementary, FcCA-labeled targets present at 25 nM in 0.5 M, pH 7 SPB, also for 1 h.Presence of hybridization, as evidenced by FcCA electroactivity in CV traces, was thus indicative of probe immobilization and hence incomplete crosslinking of PMPMS.
As shown in Figure 3, PMPMS layers crosslinked with BMDPM and BMH did not exhibit FcCA activity, indicating that probes did not immobilize.This is consistent with achievement of high degrees of crosslinking, leaving few unreacted PMPMS thiols.In contrast, layers crosslinked with TMEA and BM(PEG) 3 both exhibited FcCA peaks.This indicates that probes were able to immobilize through remnant PMPMS thiols due to incomplete crosslinking.The difference in crosslinking effectiveness between TMEA and BM(PEG) 3 on one hand and BMH and BMDPM on the other likely reflects the more hydrophilic nature of the former two, Figure 1.The more hydrophilic TMEA and BM(PEG) 3 crosslinkers likely experience greater difficulty diffusing into the hydrophobic interior of the PMPMS layer and therefore are not able to access buried PMPMS thiols despite the layer being only 2 nm thick (cf. Figure 5).Since these crosslinkers were less effective, subsequent experiments focused on the relative strengths of the BMH and BMDPM crosslinkers.by monitoring the evolution of cyclic voltammetry (CV) curves after exposure of the films to 80 ∘ C for various durations.Measurements were performed in buffered 0.2 M KCl in the presence of [Fe(CN) 6 ] 3−/4− redox couple.Previously, XPS measurements in combination with EIS analysis revealed that PMPMS films on gold are continuous above 0.8 nm [33].As the current films were nearly twice as thick (cf. Figure 5), they start as continuous and provided they remain so after the thermal treatment, without desorption or dewetting, the CV traces should remain unaltered.Such behaviour is expected if the PMPMS chains are extensively crosslinked to each other so that it is not possible for them to rearrange.On the other hand, rearrangement of the layer structure due to desorption or dewetting of PMPMS would lead to changes in film thickness and hence surface capacitance.Such changes can be monitored simply from the CV baseline current   , since   is related to capacitance as   =   ] where ] is the scan rate.Should the PMPMS layer become extensively damaged with formation of holes then a pronounced increase in ferrocyanide [Fe(CN) 6 ] 3−/4− electroactivity would also be expected.

Thermal Stability of
As shown in Figure 4(a), PMPMS films crosslinked with BMH rearranged after just a 15 min exposure to 80 ∘ C, as primarily reflected in the increase in baseline currents.Longer exposures resulted also in observation of [Fe(CN) 6 ] 3−/4− redox activity.The broad peak widths and large separation between the [Fe(CN) 6 ] 3−/4− cathodic and anodic peaks are suggestive of heterogeneity in film thickness as well as electron transfer limitations being observed at the relatively fast 20 V/s scan rates.In contrast, films crosslinked with BMDPM remained minimally affected even after over an hour at 80 ∘ C, Figure 4(b).This indicates that the BMDPM-crosslinked layer continued to fully cover the electrode surface and was not thinned or significantly damaged by heating, and did not form pinholes that would allow [Fe(CN) 6 ] 3−/4− to approach the electrode.For comparison, a control measurement on an uncrosslinked PMPMS layer is shown in Figure 4(c).
The amount of linker incorporation for both BMDPM and BMH were next investigated by monitoring the change in film thickness with time of exposure to the crosslinking solution, Figure 5. PMPMS films were dipped in the crosslinker solution for a designated time and then transferred to an aqueous buffer for determination of thickness as described in the experimental section.As seen in the figure, incorporation of both crosslinkers was complete within 5 min, with no significant change with times of up to 17 hrs.Starting from uncrosslinked thickness of about 1.4 nm, incorporation of BMDPM added about 1.0 nm while BMH caused an increase of around 0.5 nm.
Given that linker incorporation required just a few minutes, the question arises whether it is necessary to wait as long as 17 hours for crosslinking to fully develop; in other words, whether incorporated linkers react right away with a second PMPMS thiol to form a crosslink, or whether the longer time is required.Uncrosslinked PMPMS is a polymer melt, but crosslinking will render it elastic.Previously, in CV experiments using crosslinked PMPMS at various temperatures it was found that the baseline current systematically and reversibly changed with temperature [28], an observation that was attributed to changing roughness of the PMPMS film due to temperature dependence of its interfacial tension.Higher extents of crosslinking should suppress such rearrangements due to greater constraints on mobility of the PMPMS chains; this suggests that it should be possible to qualitatively track the extent of film crosslinking from the difference in its baseline current between two temperatures.Accordingly, kinetics of BMDPM crosslinking were monitored by considering the difference in baseline current between 25 ∘ C and 50 ∘ C measured in 0.5 M SPB.As shown in Figure 6, longer crosslinking times reduced the temperature-induced change in baseline current, suggesting that the extent of crosslinking continued to increase over the investigated time of up to 17 hrs.The conclusion is that although crosslinker was largely incorporated within minutes, completing the formation of crosslinks required longer durations, on the order of hours.
An important parameter is the ratio of PMPMS thiols to crosslinker maleimides.This ratio determines whether sufficient maleimides are incorporated to react with all PMPMS thiols, as well as how many maleimides remain for attachment of biomolecules.From the 1.4 nm thickness of the film before crosslinking and a molecular volume of 0.23 nm 3 per PMPMS residue, the number of PMPMS thiols within one cm 2 area of the film was (1.4 nm)(1 × 10 14 nm 2 /cm 2 )/(0.23 nm 3 ) = 6.1 × 10 14 thiols/cm 2 .Of this number, based on an earlier XPS analysis of Au-bound and unbound S 2p signals about 3.4 × 10 14 thiols/cm 2 are expected to bind to the gold support [33], leaving about (6.1 -3.4) × 10 14 = 2.7 × 10 14 thiols/cm 2 available for reacting with crosslinkers.From the change in film thickness and crosslinker molecular volumes (BMDPM: 0.41 nm 3 /molecule; BMH: 0.35 nm 3 /molecule), analogous calculations yield the amount of incorporated crosslinker as 2.5 × 10 14 molecules/cm 2 for BMDPM and 1.5 × 10 14 molecules/cm 2 for BMH.Since each crosslinker bears two maleimides, the maleimide to thiol ratio was 2(2.5 × 10 14 ) to 2.7 × 10 14 , or about 1.9:1 for BMDPM; analogous calculations yield 1.2:1 for BMH.These estimates support the conclusion that crosslinker maleimides were incorporated in excess and therefore sufficient to react with all available PMPMS thiols.This is also consistent with the negligible attachment of DNA probes through formation of disulfide bridges, Figure 3. Uncertainties in these values may be significant, however, due to approximations including use of the dielectric constant for unmodified PMPMS to also calculate thicknesses of crosslinked films.
To confirm activation of the films for further modification, remnant maleimide activity after linker incorporation was measured.BMDPM-crosslinked (1 mM crosslinker, 17 hrs reaction time) PMPMS films were reacted with 1mM 6-(ferrocenyl) hexanethiol (FSH), and then the FSH coverage 3 hr.17 hr.5 min.was quantified from integrating its CV peak, analogous to (4).These measurements yielded FSH immobilization coverage of 5 × 10 12 molecules/cm 2 , indicating that at least this coverage of maleimide was available to support further modification with biomolecules.Moreover, taking the earlier estimate of 2.7 × 10 14 thiols/cm 2 available for crosslinking, with 5 × 10 12 thiols/cm 2 reacting with BMDPM crosslinker in a monodentate fashion and the rest assumed to form crosslinks, predicts an incorporated BMDPM coverage of about 1.3 × 10 14 molecules/cm 2 .This is about half the 2.5 × 10 14 molecules/cm 2 derived from film thickness measurements.The discrepancy could reflect a number of uncertainties in these estimates, including not correcting for decrease in PMPMS dielectric constant due to crosslinking, what would overestimate the film thickness and hence incorporation of crosslinker, as well as incomplete reaction of maleimides with FSH, e.g., due to inaccessibility of some maleimides and/or their hydrolysis to unreactive maleamic acid.

Melting Curve Measurements.
Based on their excellent stability, BMDPM-crosslinked films were selected for demonstration of melting curve measurements on DNA films.Thiol-terminated DNA probes were immobilized to BMDPM-crosslinked PMPMS films, placed under a stirred solution of complementary FcCA-labeled target DNA present in excess, and the extent of hybridization was measured as a function of temperature.Melting curve measurements used a temperature scan rate of 0.3 ∘ K per minute.An example of a melting curve is shown in Figure 7.
The main feature of note in Figure 7 is the near superposition of heating and cooling curves.This superposition highlights the stability of these films over several hours at elevated temperatures of up to 348 ∘ K (75 ∘ C), since the same melting behaviour is observed for both the cooling and subsequent heating ramps.The superposition also indicates that the melting data were obtained under equilibrium conditions.The inset in Figure 7 shows a typical CV scan including the redox peaks of the FcCA-labeled DNA target.The data confirm that the DNA probes remained attached to the surface and that their hybridization activity was preserved during the heating.BMDPM-crosslinked PMPMS films are therefore well suited for applications such as study of hybridization thermodynamics on solid supports [3,4].The melting temperature on the solid support can be estimated from the 50% hybridization state as 321 ∘ K.This value can be compared to the solution prediction of 335 ∘ K calculated using UNAFOLD [38].The lower temperature for melting of immobilized duplexes is not surprising, given that hybridization on solid supports tends to be less thermodynamically favorable than that in solution [1,4,9,39].

Conclusion
This electrochemical study investigated how crosslinkers stabilize nanometer thick films of initially uncrosslinked polysiloxane polymers, which are used as base layers for attachment of biomolecules to gold.Several conclusions from this system are expected to be of general interest.First, it is essential to select crosslinkers compatible with the polymer even for very thin (approximately 2 nm thick in this study) layers.In the present study only the two more hydrophobic crosslinkers were able to react with all thiol groups within the hydrophobic polymer film.From the two hydrophobic crosslinkers that reacted, bismaleimidohexane (BMH) and 1,1  -(methylenedi-4,1-phenylene) bismaleimide (BMDPM), BMDPM produced much more robust films, as judged by stability of the films when exposed to temperatures of 80 ∘ C. The primary difference between these two crosslinkers is the more flexible nature of BMH, compared to the rather rigid ring-containing structure of BMDPM (Figure 1).A possible explanation for the effectiveness of BMDPM is that more International Journal of Electrochemistry Cooling Heating 0.0 5.0x10 11   1.0x10 12   1.5x10 12   2.0x10 12   2.5x10 12   3.0x10 12    Figure 7: A sample melting curve measured on a DNA monolayer immobilized to a BMDPM-crosslinked PMPMS film.The probe sequence is 5  -ATG ATG ATG CAG TGT GAG TT-3  , which was also used in the thermodynamic studies of reference [4].Inserted: CV curve at  = 305 K. rigid and hence extended crosslinkers are more likely to crosslink two different polymer chains as opposed to two locations on the same chain, what should better stabilize the layer.However, rather long times were required for crosslinks to fully form-up to 17 hrs-indicating that once a BMDPM crosslinker incorporated via one terminal maleimide, it can take hours for its other end to successfully react with a second thiol group.These long times may be required because some crosslinks are conformationally difficult to realize.Nevertheless, with the optimized crosslinking protocol, highly thermostable immobilization of biomolecules to solid supports is possible.

Figure 1 :
Figure 1: Chemical structures of the polythiolsiloxane polymer PMPMS and the crosslinkers tested.

Figure 2 :
Figure2: EIS data for a PMPMS layer crosslinked with BMDPM for the indicated time.Calculated fits, using an equivalent circuit of a solution resistance in series with a constant phase element, are shown for the 0 and 30 min data series as solid lines.
Crosslinked PMPMS.Next, the stability of BMH and BMDPM-crosslinked films was compared

Figure 3 :
Figure 3: PMPMS thiols remaining after crosslinking support immobilization of disulfide-terminated probes through thiol/disulfide exchange reactions, leading to hybridization signal (peak at around 0.5 V) for TMEA and BM(PEG) 3 crosslinked films.Lack of signal in BMH and BMDPM treated films indicates near complete conversion of PMPMS thiols.Conditions of PMPMS deposition: 10 mM PMPMS in DMF, 2 hrs.PMPMS crosslinking: 1 mM crosslinker in DMF, 17 hrs.

Figure 4 :
Figure 4: Cyclic voltammetry measurements on PMPMS layers crosslinked for 17 hours with 1 mM (a) BMH and (b) BMDPM in DMF.The cured layers were immersed in 80 ∘ C deionized water for the indicated time, after which they were immersed in K 3 Fe(CN) 6 solution for the CV measurements.PMPMS deposition: 10 mM PMPMS in DMF, 2 h.Data for an uncrosslinked PMPMS layer are shown in (c).

Figure 5 :
Figure 5: Kinetics of linker incorporation as indicated by changes in monolayer thickness.The error bars correspond to standard deviation of two independent EIS measurements performed on the same sample.

Figure 6 :
Figure 6: The difference in CV baseline current between two temperatures, 25 ∘ C and 50 ∘ C, in 0.5M SPB, as a function of BMDPM crosslinking time.Δ on the -axis is the temperature-induced change in the baseline separation between forward and reverse CV sweeps, Δ = (  - V ) 25C − (  - V ) 50C , measured at a bias of 0.1 V.

Table 1 :
Crosslinker properties."Arm length" refers to distance between the two maleimide C=C bonds.Arm lengths for BMH and BMDPM are predictions from Scifinder, while those for BM(PEG) 3 and TMEA were provided by the manufacturer.