International Journal of Medicinal ChemistryVolume 2011 (2011), Article ID 592879, 10 pagesdoi:10.1155/2011/592879

Research Article

Pharmacophore Modelling and Synthesis of Quinoline-3-Carbohydrazide as Antioxidants

Mustapha El Bakkali,¹ Lhassane Ismaili,² Isabelle Tomassoli,² Laurence Nicod,¹ Marc Pudlo,² and Bernard Refouvelet²

¹Laboratoire de Biologie Cellulaire Equipe 2SBP EA4267, 4 Place Saint Jacque 25030 Besançon Cedex, France²Laboratoire de Chimie Thérapeutique Equipe 2SBP EA4267, 4 Place Saint Jacque 25030 Besançon Cedex, France

Received 6 October 2010; Revised 16 December 2010; Accepted 21 January 2011

Academic Editor: Armando Rossello

Copyright © 2011 Mustapha El Bakkali et al. Mustapha El Bakkali et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract.

From well-known antioxidants agents, we developed a first pharmacophore model containing four common chemical features: one aromatic ring and three hydrogen bond acceptors. This model served as a template in virtual screening of Maybridge and NCI databases that resulted in selection of sixteen compounds. The selected compounds showed a good antioxidant activity measured by three chemical tests: DPPH radical, radical, and superoxide radical scavenging. New synthetic compounds with a good correlation with the model were prepared, and some of them presented a good antioxidant activity.

1. Introduction

Free radicals play an important role in the pathogenesis of many diseases, accounting for continuing interest in the identification and development of novel antioxidants that prevent radical-induced damage.

In humans, several pathologies involve the overproduction of reactive oxygen species (ROS): these oxygen species such as the superoxide radical anion () and hydrogen peroxide (H₂O₂) are formed by the partial reduction of molecular oxygen. Formation of the hydroxyl radical (), another ROS, is thought to occur through the one-electron reduction of H₂O₂. This reaction is facilitated by transition metals that are in a reduced valence state (e.g., reduced copper or iron) [1]. Additionally, there are a large number of other reactive species that are formed from the reaction ROS with biological molecules (e.g., polyunsaturated lipids, thiols, and nitric oxide (NO)) [2]. For example, reacts with NO to form peroxynitrite anion (ONOO⁻), which is unstable at physiological pH and rapidly decomposes. It forms potent nitrating and oxidizing species [3, 4] or hypochlorite (XOCl) that is a powerful oxidant produced by activated neutrophils via the reaction of H₂O₂ and Cl⁻, catalysed by the heme enzyme myeloperoxidase [5].

A lot of natural and synthetic products like quercetin 1, curcumine 2, resveratrol 3, Trolox 4, and N-acetylcystein 5 are known for their antioxidant activity [6–10]. Some heterocyclic compounds, either natural (phytoestrogens) or obtained by synthesis, having coumarin or quinoline rings, were studied for their biological activity. They are used especially as radicals scavenger like quercetol and coumestrol [11, 12] or the copper or iron chelating molecules such as clioquinol [13, 14].

After first studies realized in our laboratory [15–17] on new compounds with quinoline and coumarin structures and with the aim of discovering a very strong antioxidant, we decided to introduce in our research the three-dimentional generation and database searching. The increasing number of successful applications of 3D-pharmacophore-based searching in medicinal chemistry clearly demonstrates its utility in the modern drug discovery paradigm [18, 19]. In the absence of such three-dimensional structure-based, we attempted to identify the hypothetical 3D-ligand-based pharmacophore model by using the common features hypothesis generation approach (HipHop) implemented in the program Catalyst [20]. In particular, HipHop algorithm finds common feature pharmacophore models among a set of highly active compounds and carry out a qualitative model (without taking care of the activity data) which represents the essential 3D arrangement of functional groups common to the set of molecules that explains the specific activity, antioxidant in the current study.

The generation of a pharmacophore model for antioxidant from a training set of five molecules using catalyst/HipHop gave ten hypothesis, the best one was used for the databases search. The identified compounds were tested and discussed to validate a pharmacophore hypothesis.

Then, this pharmacophore was used to predict and select the synthesis of new quinoline derivatives, many compounds were prepared and their antioxidant properties evaluated by hydroxyl radical scavenging activity and by their antiradical activity against 2,2-diphenyl-1-picrylhydrazyl radical (DPPH•) and anion superoxide.

2. Result and Discussion

2.1. Training Set

Five molecules Quercetin 1, curcumine 2, resveratrol 3, Trolox 4, and N-acetyl cystein 5 as shown in the Scheme 1 were selected for the training set representing the best known natural antioxidants [5–10]. All structures were generated using editor sketcher in DS Catalyst software package and to build conformational models of up to 250 conformers for each molecule, the “best conformer generation” option and 10 kcal/mol energy cutoff were chosen.

Figure 1: Quercetin mapping with selected pharmacophore.

We employed this model as 3D-search query against the NCI, Maybridge, and minimaybridge structure databases (each contained thousands of compounds) using the “fast flexible search” approach implemented within Catalyst. The pharmacophore captured 300 hits for each database, we selected sixteen compounds Scheme 2 on the basis of fit value and availability.

Figure 2: Compound 8c mapping with a pharmacophore.

The synthetic route to prepare desired substituted 4-hydroxy-2-oxophenylmethylene-1,2-dihydroquinolin-3-carbohydrazide is described in Scheme 4.

Scheme 4: Synthetic route to prepare compounds 8.

The condensation N-H or N-methyl anhydride isatoique with ethylmalonate in dimethylformamide gave ethyl 4-hydroxy-2-oxo-1,2-dihydroquinoline-3-carboxylate 6a or his derivatives N-methyl 6b [25]. The 6a and 6d were converted in resulting 7a and 7b with hydrazine hydrate in methanol, finally they reacted with different aldehydes. This procedure gave compounds 8a–c, as a mixtures of E-and Z-isomers in a E : Z : 9 : 1, 7 : 3 and 4 : 1 ratio, respectively, whereas the target molecules 8d–h were isolated as pure E isomers. The E configuration compounds 8 was characterized in the 2D-NMR (¹H-¹H) spectra by NOESY experiments, and analyzing by the NOE effects on the hydrogens for the NH amide of carbohydrazide moiety, and the CH of imines.

All the compounds summarized in (Table 3) were obtained in moderate to good yields ranging from 56% to 94%. All these products were isolated from reaction mixture by recrystallisation from ethanol, and their structures were characterized by ¹H NMR, IR spectra and elementary analysis.

Table 3: Description and fit value of pharmacophore mapping of compounds 8a–h.

Compounds
R
R1
R2
R3
Fit value

8a
H
2-OH
4-OH
H
3.2

8b
H
2-OH
5-CH3
H
3.0

8c
H
2-OH
5-OCH3
H
3.3

8d
H
2-Cl
3-NO2
6-Cl
2.6

8e
CH3
2-Cl
3-NO2
6-Cl
2.8

8f
CH3
2-OH
5-CH3
H
2.7

8g
CH3
2-OH
5-OCH3
H
3.1

8h
CH3
2-NO2
H
H
2.7

Fit value represents a good match of features of the pharmacophore model with the ligand.

The antioxidant activity for these compounds was measured by two methods DPPH and anion superoxide (Table 4), the hydroxyl radical scavenging is not applicable for these compounds because of their fluorescence at the studied wavelength (407 nm emission with excitation at 305 nm).

Table 4: DPPH radical and Superoxide radical scavenging of synthetic compounds.

Compounds
Concentration μmol·L −1

50 μmol·L −1
100 μmol·L −1
150 μmol·L −1

% RSA*
DPPH

DPPH

DPPH

8a

8b

8c

54.7

8d

8e

8f

8g

8h

*RSA: Radical Scavenging Activity.

All synthesized compounds exhibit antiradical activity against DPPH radical and anion superoxide tests. The products 8a and 8c having a good fit value present better results. The product 8h has a lower result. It’s probably due to absence of hydroxyl group

Conclusion. The present study is a successful example for a rational identification of antioxidants agents. This was accomplished by generating a three-dimensional pharmacophore model based on a training set of five well-know antioxidants. The model containing one aromatic group and three hydrogen bond acceptors was selected and used to identify new quinoline derivatives antioxidant agents.

4. Experimental

4.1. Antioxidant Activity Studies

Assay of Hydroxyl Radical () Scavenging ActivityIn a screw-capped test tube, 0.2 mL of sodium benzoate (10 mmol), 0.2 mL of FeSO₄·7H₂0 (10 mmol) and EDTA (10 mmol) were added. Then the sample solution and a phosphate buffer (pH 7.4, 0.1 mol) were mixed to give a total volume of 1.8. Finally, 0.2 mL of H₂O₂ solution (10 mmol) was added, and the whole incubated at 37°C for 2 h. After incubation, the fluorescence was measured on spectrofluorimeter Shimadzu RF 10AXL at wavelengths 407 nm for emission and 305 nm for excitation.

DPPH Radical Scavenging ActivityThe capacity of compounds to scavenge the “stable” free radical DPPH was monitored according to the method of Hatano et al. [26]. Various concentrations of methanolic compounds solutions (0.3 mL) were mixed with methanolic solution containing DPPH radicals (1.5·10⁻⁴ M, 2.7 mL). The mixture was shaken vigorously and left to stand for 2 h in the dark (until stable absorption values were obtained). The reduction of the DPPH radical was determined by measuring the absorbance at 517 nm. The RSA was calculated as a percentage of DPPH colouration using where is the absorbance of the solution when the compound has been added at a particular level and is the absorbance of the DPPH solution. Mean values from three independent samples were calculated for each compound and standard deviations were less than 5%.

Radical Scavenging ActivityThe reaction mixture (1 mL) contained 700 μL of various concentrations of methanolic compounds solutions, 100 μL of β-NADH (1 mM in water), 100 μL of NBT (1 mM in 1 M-phosphate buffer, pH 7.8 and 100 μL of PMS (120 μM in water) added in that order and the mixture allowed to react at RT for 10 min. The control contained all the reaction reagents except the test material. The reaction was terminated by adding 40 μL of concentrated HCl (10 mM) and absorbance was measured at 560 nm against blanks that contained all compound except test material and PMS.The percentage scavenging effects was calculated from the decrease in absorbance against control.

4.2. Computational Methods

All molecular modelling studies were performed using discovery studio 2.1 with catalyst module. All structures were generated using 2D/3D editor sketcher and minimized to the closest minimum using the CHARMm-like force field implemented in the program [27]. A stochastic research coupled to a poling method [28] was applied to generate conformers for each compound by using “Best conformer generation” option with a 20 kcal/mol energy cutoff (20 kcal/mol maximum compared to the most stable conformer).

The pharmacophore-based investigation involved using the catalyst/Hip/Hop program to generate feature based 3D pharmacophore alignments [29]. This was performed in a three step procedures: (a) a conformation model of each molecule in the training set was generated, (b) each conformer was examined for the presence of certain chemical features, (c) a three dimensional configuration of chemical feature these steps were performed with a module common feature pharmacophore generation.

4.3. Synthesis

4.3.1. General Methods

Reactions were monitored by TLC using precoated silica gel aluminum plates containing a fluorescent indicator (Macherey-Nagel). Detection was done with UV (254 nm). Melting points were determined on a Kofler block and were uncorrected. Infrared spectra were recorded on a Shimadzu FTIR-8201 PC spectrometer in KBr ( in cm⁻¹). ¹H NMR spectra were recorded on a Bruker AC 300 spectrometer. Microanalyses were carried out by the Service Central d’Analyses, CNRS, Vernaison (France). All reagents were pure analytical grades and used without further purification.

4.3.2. General Method of Preparation of Compounds 6a-b

The corresponding anhydride isatoic (1 eq) was suspended in DMF (10 mL) at 0°C. Sodium hydride (2 eq) and diethyl malonate (5 eq) were added slowly. The reaction mixture was heated at 85°C for 5 h. Then, 10 mL of water were added and the mixture was acidified with concentrated hydrochloric acid. The resulting solid was filtered, washed with water and dried, yielding the desired compound.

4.3.3. Ethyl 4-hydroxy-2-oxo-1,2-dihydroquinoline-3-carboxylate (6a)

Following the General procedure in Section 4.3.2, the reaction of isatoic anhydride (1 g, 6.13 mmol) with NaH (0.29 g, 12.3 mmol), and diethyl malonate (4.91 g, 30.7 mmol) gave product 6a (1 g, 70%): mp 134°C; IR (KBr) 3406, 3193, 1658, 1604 cm⁻¹; ¹H NMR (DMSO-, 300 MHz) 11.47 (s, 1H), 7.94 (d,  Hz, 1H), 7.62 (t,  Hz, 1H), 7.27 (d,  Hz, 1H), 7.20 (t,  Hz, 1H), 4.34 (q,  Hz, 2H), 1.31 (t,  Hz, 3H). Anal. Calcd. for C₁₂H₁₁NO₄: C, 61.80%; H, 4.75%; N, 6.01%. Found: C, 61.72%; H, 4.78%; N, 6.10%.

4.3.4. Ethyl 4-hydroxy-1-methyl-2-oxo-1,2-dihydroquinoline-3-carboxylate (6b)

Following the General procedure in Section 4.3.2, the reaction of N-methyl anhydride isatoic (1 g, 5.65 mmol) with NaH (0.27 g, 11.3 mmol), and diethyl malonate (4.52 g, 28.2 mmol) gave product 6b (0.56 g, 41%): mp 104°C; IR (KBr) 1631, 1593, 1562 cm⁻¹; ¹H NMR (DMSO-, 300 MHz) 8.05 (d,  Hz, 1H), 7.45 (t,  Hz, 1H), 7.52 (d,  Hz, 1H), 7.31 (t,  Hz, 1H), 4.33 (q,  Hz, 2H), 3.54 (s, 3H), 1.30 (t,  Hz, 3H). Anal. Calcd. for C₁₃H₁₃NO₄: C, 63.15%; H, 5.30%; N, 5.67%. Found: C, 63.24%; H, 5.27%; N, 5.61%.

4.3.5. General Method for Compounds 7a-b

The quinoline-3-carboxylate (1 eq) and its derivatives were suspended in methanol (20 mL). hydrazine (1.5 eq) was added and the mixture was heated at 100°C for 30 min. The precipitated compound was collected by filtration and used without further purification.

4.3.6. Hydroxy-2-oxo-1,2-dihydroquinoline-3-carbohydrazide (7a)

Following the General procedure in Section 4.3.5 the reaction of hydrazine (0.52 g, 16.3 mmol) with compound 6a (2 g, 8.58 mmol) gave product 7a (1.57 g, 84%): mp > 260°C; IR (KBr) 3167, 1674, 1616, 1531 cm⁻¹; ¹H NMR (DMSO-, 300 MHz) 11.89 (s, 1H), 10.97 (s, 1H), 7.97 (d,  Hz, 1H), 7.68 (t,  Hz, 1H), 7.36 (d,  Hz, 1H), 7.29 (t,  Hz, 1H), 2.79 (s, 2H). Anal. Calcd. for C₁₀H₉N₃O₃: C, 54.79%; H, 4.14%; N, 19.17%. Found: C, 54.85%; H, 4.08%; N, 19.90%.

4.3.7. 4-Hydroxy-1-methyl-2-oxo-1,2-dihydroquinoline-3-carbohydrazide (7b)

Following the General procedure in Section 4.3.5 the reaction of hydrazine (0.48 g, 15 mmol) with compound 6b (2 g, 8.10 mmol) gave product 7b (1.20 g, 64%): mp > 260°C; IR (KBr) 3328, 3240, 1647, 1589 cm⁻¹; ¹H NMR (DMSO-, 300 MHz) 11.00 (s, 1H), 8.01 (d,  Hz 1H), 7.81 (t,  Hz, 1H), 7.62 (d,  Hz, 1H), 7.38 (t,  Hz, 1H), 4.95 (s, 2H), 3.63 (s, 3H). Anal. Calcd. for C₁₁H₁₁N₃O₃: C, 56.65%; H,4.75%; N, 18.02%. Found: C, 56.52%; H, 4.79%; N, 18.11%.

4.3.8. General Method for Compounds (8a–h)

The corresponding quinoline-3-carboxyhydrazides 7a-b were stirred with 2,4-dihydroxybenzaldehyde or its derivatives in dimethyl sulfoxide and four drops of orthophosphoric acid for 15 min at room temperature. The mixture was then heated at 100°C for 1 h. The compound was collected by filtration and washed with water.

4.3.9. N′-[(E)-(2,4-Dihydroxyphenyl)methylidene]-4-hydroxy-2-oxo-1,2-dihydroquinoline-3-carbohydrazide (8a)

Following the General procedure in Section 4.3.8, the reaction of 2,4-dihydroxybenzaldehyde (0.31 g, 2.3 mmol) with compound 7a (0.5 g, 2.3 mmol) gave product 8a (0.75 g, 97%): mp > 260°C; IR (KBr) 3205, 1658, 1558 cm⁻¹; ¹H NMR (DMSO-, 300 MHz) 13.19 (s, 1H), 12.01 (s, 1H), 11.08 (s, 1H), 10.10 (s, 1H), 8.51 (s, 1H), 7.96 (d,  Hz, 1H), 7.66 (t,  Hz, 1H), 7.34 (t,  Hz, 2H), 7.27 (t,  Hz, 1H), 6.34 (d,  Hz, 1H), 6.29 (s, 1H). Anal. Calcd. for C₁₇H₁₃N₃O₅: C, 60.18%; H, 3.86%; N, 12.38%. Found: C, 60.24%; H, 3.84%; N, 12.31%.

4.3.10. 4-Hydroxy-N′-[(1E)-(2-hydroxy-5-methylphenyl)methylene]-2-oxo-1,2-dihydroquinoline-3-carbohydrazide (8b)

Following the General procedure in Section 4.3.8, the reaction of 2-hydroxy-5-methyl-benzaldehyde (0.15 g, 1.10 mmol) with compound 7a (0.25 g, 1.14 mmol) gave product 8b (0.31 g, 81%): mp > 260°C, IR (KBr) 3001, 1651, 1612, 1581, 1546 cm⁻¹; ¹H NMR (DMSO-, 300 MHz) 13.65 (s, 1H), 8.87 (s, 1H), 8.27 (d,  Hz, 1H), 7.97 (t,  Hz, 1H) 7.67 (s, 1H), 7.57 (t,  Hz, 1H), 7.39 (d,  Hz, 1H), 7.11 (t,  Hz, 1H), 2.75 (s, 3H). Anal. Calcd. for C₁₈H₁₅N₃O₄: C, 64.09%; H, 4.48%; N, 12.46%. Found: C, 64.12%; H, 4.46%; N, 12.38%.

4.3.11. 4-Hydroxy-N′-[(1E)-(2-hydroxy-5-methoxyphenyl)methylene]-2-oxo-1,2-dihydroquinoline-3-carbohydrazide (8c)

Following the General procedure in Section 4.3.8, the reaction of 2-hydroxy-5-methoxy-benzaldehyde (0.28 g, 1.84 mmol) with compound 7a (0.40 g, 1.83 mmol) gave product 8c (0.59 g, 92%): mp > 260°C; IR (KBr) 3355, 1670, 1651, 1577, 1542 cm⁻¹; ¹H NMR (DMSO-, 300 MHz) 13.41 (s, 1H), 10.43 (s, 1H), 8.92 (s, 1H), 8.58 (s, 1H), 7.98 (d,  Hz, 1H), 7.68 (t,  Hz, 1H), 7.36 (d,  Hz, 1H), 7.23 (m, 1H), 7.12 (d,  Hz, 1H), 6.90 (m, 3H), 3.69 (s, 3H). Anal. Calcd. for C₁₈H₁₅N₃O₅: C, 61.19%; H, 4.28%; N, 11.89%. Found: C, 61.26%; H, 4.25%; N, 11.82%.

4.3.12. N′-[(1E)-(2,6-Dichloro-3-nitrophenyl)methylene]-4- hydroxy-2-oxo-1,2-dihydroquinoline-3-carbohydrazide (8d)

Following the General procedure in Section 4.3.8, the reaction of 2,6-dichloro-3-nitro-benzaldehyde (1 g, 4.55 mmol) with compound 7a (0.50 g, 2.30 mmol) gave product 8d (0.87 g, 92%): mp > 260°C; IR (KBr) 3298, 3093, 1735, 1620, 1596, 1539 cm⁻¹; ¹H NMR (DMSO-, 300 MHz) 13.92 (s, 1H), 11.80 (s, 1H), 8.60 (s, 1H), 8.16 (d,  Hz, 1H), 8.01 (d,  Hz, 1H), 7.88 (d,  Hz, 1H), 7.66 (s, 1H), 7.35 (s, 1H), 7.26 (s, 1H). Anal. Calcd. for C₁₇H₁₀Cl₂N₄O₅: C, 48.48%; H, 2.39%; Cl, 16.83%; N, 13.30%. Found: C, 48.44%; H, 2.37%; Cl, 16.86%; N, 13.25%.

4.3.13. N′-[(1E)-(2,6-Dichloro-3-nitrophenyl)methylene]-4-hydroxy-1-methyl-2-oxo-1,2-dihydroquinoline-3-carbohydrazide (8e)

Following the General procedure in Section 4.3.8, the reaction of 2,6-dichloro-3-nitro-benzaldehyde (0.78 g, 3.55 mmol) with compound 7b (0.30 g, 1.28 mmol) gave product 8e (0.51 g, 91%): mp 230°C; IR (KBr) 3058, 1635, 1577, 1558, 1519 cm⁻¹; ¹H NMR (DMSO-, 300 MHz) 8.66 (s, 1H), 8.15 (d,  Hz, 2H), 7.87 (d,  Hz, 1H), 7.79 (s, 1H), 7.60 (s, 1H), 7.36 (s, 1H), 3.64 (s, 3H). Anal. Calcd. for C₁₈H₁₂Cl₂N₄O₅: C, 49.67%; H, 2.78%; Cl, 16.29%; N, 12.87%. Found: C, 49.71%; H, 2.76%; Cl, 16.31%; N, 12.82%.

4.3.14. 4-Hydroxy-N′-[(1E)-(2-hydroxy-5-methylphenyl)methylene]-1-methyl-2-oxo-1,2-dihydroquinoline-3-carbohydrazide (8f)

Following the General procedure in Section 4.3.8, the reaction of 2-hydroxy-5-methyl-benzaldehyde (0.15 g, 1.10 mmol) with compound 7b (0.25 g, 1.07 mmol) gave product 8f (0.34 g, 93%): mp > 260°C; IR (KBr) 2985, 1624, 1589, 1558, 1519 cm⁻¹; ¹H NMR (DMSO-, 300 MHz) 12.55 (s, 1H), 9.95 (s, 1H), 7.81 (s, 1H), 7.30 (d,  Hz, 1H), 7.00 (t,  Hz, 1H), 6.82 (d,  Hz, 1H), 6.58 (d,  Hz, 1H), 6.53 (s, 1H), 6.30 (d,  Hz, 1H), 6.01 (d,  Hz, 1H), 2.83 (s, 3H), 1.41 (s, 3H). Anal. Calcd. for C₁₉H₁₇N₃O₄: C, 64.95%; H, 4.88%; N, 11.96%. Found: C, 64.92%; H, 4.86%; N, 11.95%.

4.3.15. 4-Hydroxy-N′-[(1E)-(2-hydroxy-5-methoxyphenyl)methylene]-1-methyl-2-oxo-1,2-dihydroquinoline-3-carbohydrazide (8g)

Following the General procedure in Section 4.3.8, the reaction of 2-hydroxy-5-methoxy-benzaldehyde (0.16 g, 1.05 mmol) with compound 7b (0.25 g, 1.07 mmol) gave product 8g (0.33 g, 84%): mp > 260°C; IR (KBr) 2966, 1643, 1562, 1535 cm⁻¹: ¹H NMR (DMSO-, 300 MHz) 13.49 (s, 1H), 10.51 (s, 1H), 8.65 (s, 1H), 8.15 (d,  Hz, 1H), 7.82 (d,  Hz, 1H), 7.65 (d,  Hz, 1H), 7.40 (t,  Hz, 1H), 7.15 (s, 1H), 6.92 (m, 2H) 3.74 (s, 3H), 3.67 (s, 3H). Anal. Calcd. for C₁₉H₁₇N₃O₅: C, 62.12%; H, 4.66%; N, 11.44%. Found: C, 62.19%; H, 4.63%; N, 11.41%.

4.3.16. 4-Hydroxy-1-methyl-N′-[(1E)-(2-nitrophenyl)methylene]-2-oxo-1,2-dihydroquinoline-3-carbohydrazide (8h)

Following the General procedure in Section 4.3.8, the reaction of 2-nitrobenzaldehyde (0.78 g, 5.16 mmol) with compound 7b (0.20 g, 0.86 mmol) gave product 8h (0.29 g, 92%): mp > 260°C; IR (KBr) 1670, 1566, 1523 cm⁻¹; ¹H NMR (DMSO-, 300 MHz) 8.67 (s, 1H), 8.15 (s, 1H), 8.11 (d,  Hz, 1H), 8.07 (s, 1H), 7.83 (t,  Hz, 1H), 7.67 (m, 2H), 7.48 (s, 1H), 7.26 (s, 1H), 3.58 (s, 3H). Anal. Calcd. for C₁₈H₁₄N₄O₅: C, 59.02%; H, 3.85%; N, 15.29%. Found: C, 59.11%; H, 3.82%; N, 15.25%.

Acknowledgments

The authors thank the “Conseil Regional de Franche-Comte” for their financial support and NCI database for chemical compounds.

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