International Journal of Medicinal Chemistry

Volume 2013 (2013), Article ID 986536, 6 pages

http://dx.doi.org/10.1155/2013/986536

Research Article

Novel Antimicrobial Agents: Fluorinated 2-(3-(Benzofuran-2-yl) pyrazol-1-yl)thiazoles

Hanan A. Mohamed,¹ Ehab Abdel-Latif,² Bakr F. Abdel-Wahab,¹ and Ghada E. A. Awad³

¹Applied Organic Chemistry Department, National Research Centre, Dokki, Giza 12622, Egypt

²Department of Chemistry, Faculty of Science, Mansoura University, Mansoura 35516, Egypt

³Chemistry of Natural and Microbial Products, National Research Center, Dokki, Giza 12622, Egypt

Received 29 May 2013; Revised 10 August 2013; Accepted 11 August 2013

Academic Editor: Armando Rossello

Copyright © 2013 Hanan A. Mohamed et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract.

A new series of 2-pyrazolin-1-ylthiazoles 8a–d and 13–16 was synthesized by cyclization of N-thiocarboxamide-2-pyrazoline with different haloketones and 2,3-dichloroquinoxaline. The structures of the new compounds were confirmed by elemental analyses as well as NMR, IR, and mass spectral data. The newly synthesized compounds were evaluated for their antimicrobial activities, and also their minimum inhibitory concentration (MIC) against most of test organisms was performed. Amongst the tested ones, compound 8c displayed excellent antimicrobial activity.

1. Introduction

Pyrazolines are nitrogen-containing heterocyclic compounds, well known for their pronounced biological activity. These biological activities include antibacterial [1], antifungal [2], herbicidal [3], and insecticidal activities [4]. It was demonstrated that the combination of pyrazole with azole ring, linked to each by one sigma bond, led to more biologically active targets; for example, pyrazolylthiazoles showed excellent antimicrobial activities [5]. Continuing our work in this research field [6–9] and in an attempt to identify new and potent antimicrobial agents, we tried here to generate new benzofuryl 2-pyrazolin-1-ylthiazoles as antimicrobial agents using simple methods.

2. Results and Discussion

2.1. Chemistry

The starting pyrazoline-1-carbothioamide 5 was prepared by treatment of 2-acetylbenzofuran 1 with equivalent of 4-flurobenzaldehyde 2 in the presence of 10% alcoholic NaOH in 90% ethanol with stirring at room temperature to give chaconne 3. Reaction of chalcone 3 with equivalent amount of thiosemicarbazide was performed in ethanol in the presence of 2.5 equivalent of sodium hydroxide to the target precursor 5.

The resulting pyrazoline-1-carbothioamide 5 was cyclized to the corresponding 2-(3-(benzofuran-2-yl)-5-(4-fluorophenyl)-4,5-dihydro-1H-pyrazol-1-yl)-4-methyl-5-(p-subs.phenyldiazenyl)thiazole derivatives 8a–d by reaction with hydrazonoyl halides 6a–d in anhydrous ethanol and in the presence of an equivalent of triethylamine (Scheme 1).

Scheme 1

The reaction product structures were elucidated by means of NMR, MS spectroscopy, and elemental analyses (Table 1).

Table 1: Characteristic data of the synthesized compounds.

Entry
Mol. formula

(M.Wt)
Calcd.

Found

C%
H%
N%

5
C₁₈H₁₄FN₃OS

(339.39)
63.70 

64.03
4.16 

4.20
12.38 

12.51

8a
C₂₇H₂₀FN₅OS

(481.54)
67.34 

67.54
4.19 

4.31
14.54 

14.35

8b
C₂₇H₁₉F₂N₅OS

(499.53)
64.92 

64.98
3.83 

3.90
14.02 

14.22

8c
C₂₇H₁₉ClFN₅OS

(515.99)
62.85 

62.94
3.71 

3.83
13.57 

13.66

8d
C₂₇H₁₉BrFN₅OS

(560.44)
57.86 

57.89
3.42 

3.39
12.50 

12.59

13a
C₂₆H₁₇ClFN₃OS

(473.95)
65.89 

65.93
3.62 

3.59
8.87 

8.93

13b
C₂₆H₁₇BrFN₃OS

(518.40)
60.24 

60.30
3.31 

3.39
8.11 

8.31

14
C₂₈H₁₈FN₃O₂S

(479.52)
70.13 

70.26
3.78 

3.82
8.76 

8.89

15
C₂₉H₁₈FN₃O₃S

(507.53)
68.63 

68.77
3.57 

3.66
8.28 

8.33

16
C₂₆H₁₆FN₅OS

(465.50)
67.08 

67.14
3.46 

3.50
15.04 

15.11

For example, ¹H NMR spectra of 8a–d contained two doublet-doublet and one triplet signals due to the presence of CH₂ adjacent asymmetric carbon. The mass spectra of 8a–d showed the molecular ion peaks at m/z 481, 499, 515 and 561, respectively in agreement with the calculated masses.

The reaction between pyrazoline-1-carbothioamide 5 and the equivalent amount of α-haloketones, for example, phenacyl bromides derivatives, 2-bromoacetylbenzofuran, and 3-bromoacetylcoumarin, was performed in refluxing ethanol to yield pyrazol-1-ylthiazoles 13–15 in good yields via the intermediates A (Scheme 2). Also, pyrazoline-1-carbothioamide 5 reacted with 2,3-dichloroquinoxaline to give pyrazolin-1-ylthiazolo[5,4-b]quinoxaline 16 in moderate yield.

Scheme 2

The ¹H NMR of compounds 13–16 indicate the disappearance of NH₂ signal due to blocking of NH₂ with thiazole nucleus. The mass spectra of 13a,b–16 showed the molecular ion peaks at m/z 473, 518, 479, 507, and 465, respectively in agreement with the calculated masses.

2.2. Biological Activity

2.2.1. Antimicrobial Activity

All the new synthesized compounds were screened for their antibacterial and antifungal activities at 100 μg/mL concentration against four Gram-positive bacteria (Staphylococcus aureus ATCC 29213; B. subtilis ATCC6633; B. megaterium ATCC 9885; Sarcina lutea), three Gram-negative bacteria (Klebsiella pneumoniae ATCC13883; Pseudomonas aeruginosa ATCC27953; E. coli ATCC 25922), and two yeast (Saccharomyces cerevisiae and Candida albicans NRRL Y-477). Ciprofloxacin and ketoconazole were respectively used as standard antibacterial and antifungal references, respectively. Most of the newly synthesized compounds showed good antimicrobial activities with respect to the control drugs. The results of antimicrobial activities were shown in Table 2. Data showed that most of compounds have superior significant antifungal potency to antibacterial potency. Compound 8c exhibited the highest potency against all tested organisms with respect to reference drugs. Compounds 8d and 13b inhibited the growth of Staphylococcus aureus ATCC 29213 with inhibition zones 23, 22 mm, respectively, while compound 5 showed excellent activities against Klebsiella pneumoniae ATCC13883; Pseudomonas aeruginosa ATCC27953; and E. coli ATCC 25922 with inhibition zone about 24 mm. Also, compound 8c showed the highest activity against Staphylococcus aureus ATCC 29213, Saccharomyces cerevisiae, and Candida albicans NRRL Y-477 with inhibition zone about 23 mm.

Table 2: Antimicrobial activity expressed as inhibition diameter zones in millimeters (mm) of chemical compounds against the pathological strains based on well diffusion assay.

Chem. compd.
Gram-positive bacteria
Gram-negative bacteria
Yeast

Staphylococcus aureus ATCC29213
B. subtilis ATCC6633
B. megaterium ATCC9885
Sarcina lutea
Klebsiella pneumoniae ATCC13883
Pseudomonas aeruginosa ATCC27953
E. coli ATCC25922
Saccharomyces cerevisiae
Candida albicans NRRL Y-477

5
18
17
20
23
24
23
24
22
18

8a
19
16
18
18
17
19
23
20
18

8b
19
18
17
28
18
19
24
21
18

8c
25
26
23
30
34
33
31
22
23

8d
23
12
17
18
16
18
24
20
18

13a
22
18
28
21
23
19
23
20
16

13b
20
18
19
22
20
21
24
19
16

14
19
18
20
23
22
22
25
24
20

15
20
17
20
24
21
19
25
20
18

16
19
16
21
20
21
20
27
21
16

Ciprofloxacin
20
22
24
20
25
24
23
N.A.
N.A.

Ketoconazole
N.A.
N.A.
N.A.
N.A.
N.A.
N.A.
N.A.
23
22

The experiment was carried out in triplicate, and the average zone of inhibition was calculated; N.A. (no activity).

2.2.2. Minimum Inhibitory Concentration (MIC)

The minimum inhibitory concentration (MIC) of the synthesized compounds against highly inhibited organisms is reported in Table 3. Compounds 5 revealed low MIC (200 μg/mL) against Staphylococcus aureus ATCC 29213, B. megaterium ATCC 9885, and Candida albicans NRRL Y-477, respectively. On the other hand, compound 8a exhibited high MIC (16 μg/mL) against B. subtilis ATCC6633 (Table 3).

Table 3: Minimum inhibitory concentration (μg/mL) against the pathological strains based on twofold serial dilution technique.

Chem. compds
Gram-positive bacteria
Gram-negative bacteria
Yeast

Staphylococcus aureus ATCC29213
B. subtilis ATCC6633
B. megaterium ATCC9885
Sarcina lutea
Klebsiella pneumoniae ATCC13883
Pseudomonas aeruginosa ATCC27953
E. coli ATCC25922
Saccharomyces 

cerevisiae
Candida albicans NRRL Y-477

5
200
—
200
100
100
100
100
100
200

8a
19
16
200
100
—
200
100
200
200

8b
100
200
—
50
200
200
100
200
—

8c
50
50
100
50
25
50
25
200
100

8d
50
—
—
100
—
200
100
200
200

13a
200
200
200
100
200
200
100
200
—

13b
100
200
50
100
100
200
100
200
—

14
200
200
200
100
100
100
50
100
200

15
200
—
200
10 0
100
200
50
200
200

16
200
—
20
100
100
200
50
200
—

Ciprofloxacin
25
25
25
25
25
25
25
N.A.
N.A.

Ketoconazole
N.A.
N.A.
N.A.
N.A.
N.A.
N.A.
N.A.
25
25

3. Experimental

3.1. Chemistry

All melting points were taken on Electrothermal IA 9000 series digital melting point apparatus. Elemental analytical data were carried from the microanalytical unit, Cairo University, Giza, Egypt. The IR spectra were recorded in potassium bromide disks on a JASCO FT/IR-6100. ¹H NMR spectra were run on JOEL-ECA 500 MHz in deuterateddimethyl sulphoxide (DMSO-d₆). Chemical shifts values (δ) are given in parts per million (ppm). The mass spectra were performed using mass Varian MAT CH-5 spectrometer at 70 eV. (E)-1-(benzofuran-2-yl)-3-(4-fluorophenyl)prop-2-en-1-one 3 [10]; hydrazonoyl halides [11]; 1-(benzofuran-2-yl)-2-bromoethanone 9 [12]; 3-(2-bromoacetyl)-2H-chromen-2-one 10 [13]; and 2,3-dichloroquinoxaline [14] were prepared according to the literature.

3.1.1. 3-(Benzofuran-2-yl)-5-(4-fluorophenyl)-4,5-dihydro-1H-pyrazole-1-carbothioamide (5)

To a suspension of chalcone 3 (10 mmol, 2.66 g) and sodium hydroxide (25 mmol, 1.0 g) in ethanol (50 mL), thiosemicarbazide (12 mmol, 1.1 g) was added. The mixture was refluxed for 12 h, then left to cool; the solid product was filtered off, washed with ethanol, and dried.

Yield 52%; m.p. 260-2°C; IR (KBr) νmax/cm⁻¹ 3460, 3335 (NH₂); ¹H NMR (DMSO-d₆) δ 3.09, 3.14 (dd, 1H, CH, J = 3.05 Hz, J = 3.05 Hz), 3.94, 4.06 (dd, 1H, CH, J = 11.45 Hz, J = 11.5 Hz), 5.88 (t, 1H, CH, J = 3.05 Hz, J = 7.65 Hz), 7.13–7.43 (m, 9H, Ar-H), 9.44 (s, 2H, NH₂, D₂O-exchangeable); MS m/z (%): 339 (M⁺, 75), 60 (100).

3.1.2. General Procedure for Compounds 8a–d; 13a–d; 14; 15; and 16

To a suspension of compound 5 (1 mmol, 0.34 g) in ethanol (20 mL), the 1 mmol of appropriate reagent {(appropriate hydrozonoyl chlorides 6 + Et₃N); (appropriate phenacyl bromides 9); (2-bromoacetylbenzofuran 10); (3-bromoacetylcoumarin 11); or (3,4-dichloroquinoxaline 12)} was added and heated under reflux for 4 h. After cooling, the precipitate was collected by suction filtration.

3.1.3. 2-(3-(Benzofuran-2-yl)-5-(4-fluorophenyl)-4,5-dihydro-1H-pyrazol-1-yl)-4-methyl-5-(phenyldiazenyl)thiazole (8a)

Yield 58%; m.p.180-2°C; ¹H NMR (DMSO-d₆) δ 2.54 (s, 3H, CH₃), 4.09, 4.11 (2dd, 2H, CH, J = 10.7 Hz, J = 9.95 Hz), 5.87 (t, 1H, CH, J = 10.7 Hz, J = 9.95 Hz), 7.18–7.73 (m, 14H, Ar-H); MS m/z (%): 481 (M⁺, 75), 95 (100).

3.1.4. 2-(3-(Benzofuran-2-yl)-5-(4-fluorophenyl)-4,5-dihydro-1H-pyrazol-1-yl)-5-((4-fluorophenyl)diazenyl)-4-methylthiazole (8b)

Yield 66%; m.p. 201-3°C; ¹H NMR (DMSO-d₆) δ 2.54 (s, 3H, CH₃), 4.07, 4.12 (2dd, 2H, CH, J = 10.7 Hz, J = 9.95 Hz), 5.89 (t, 1H, CH, J = 10.7 Hz, J = 9.95 Hz), 7.19–7.73 (m, 13H, Ar-H); MS m/z (%): 499 (M⁺, 80), 95 (100).

3.1.5. 2-(3-(Benzofuran-2-yl)-5-(4-fluorophenyl)-4,5-dihydro-1H-pyrazol-1-yl)-5-((4-chlorophenyl)diazenyl)-4-methylthiazole (8c)

Yield 72%; m.p. 206-8°C; ¹H NMR (DMSO-d₆) δ 2.53 (s, 3H, CH₃), 4.10, 4.34 (2dd, 2H, CH, J = 10.7 Hz, J = 9.95 Hz), 5.89 (t, 1H, CH, J = 10.7 Hz, J = 9.95 Hz), 7.19–7.73 (m, 13H, Ar-H); MS m/z (%): 515 (M⁺, 70), 95 (100).

3.1.6. 2-(3-(Benzofuran-2-yl)-5-(4-fluorophenyl)-4,5-dihydro-1H-pyrazol-1-yl)-5-((4-bromophenyl)diazenyl)-4-methylthiazole (8d)

Yield 75%; m.p. 128-30°C; ¹H NMR (DMSO-d₆) δ 2.55 (s, 3H, CH₃), 4.10, 4.34 (2dd, 2H, CH, J = 10.7 Hz, J = 9.95 Hz), 5.87 (t, 1H, CH, J = 10.7 Hz, J = 9.95 Hz), 7.19–7.72 (m, 13H, Ar-H); MS m/z (%): 561 (M⁺, 62), 95 (100).

3.1.7. 2-(3-(Benzofuran-2-yl)-5-(4-fluorophenyl)-4,5-dihydro-1H-pyrazol-1-yl)-4-(4-chlorophenyl)thiazole (13a)

Yield 49%; m.p. 176-8°C; ¹H NMR (DMSO-d₆) δ 4.01–4.05 (dd, 2H, CH₂, J = 11.45 Hz, J = 10.33 Hz), 5.73 (t, 1H, CH, J = 5.35 Hz, J = 6.1 Hz), 7.17–7.92 (m, 14H, Ar-H); MS m/z (%): 473 (M⁺, 80), 91 (100).

3.1.8. 2-(3-(Benzofuran-2-yl)-5-(4-fluorophenyl)-4,5-dihydro-1H-pyrazol-1-yl)-4-(4-bromophenyl)thiazole (13b)

Yield 58%; m.p. 208-10°C; ¹H NMR (DMSO-d₆) δ 4.01–4.08 (dd, 2H, CH₂, J = 11.45 Hz, J = 10.33 Hz), 5.71 (t, 1H, CH, J = 5.35 Hz, J = 6.1 Hz), 7.17–7.92 (m, 14H, Ar-H); MS m/z (%): 518 (M⁺, 49), 91 (100).

3.1.9. 4-(Benzofuran-2-yl)-2-(3-(benzofuran-2-yl)-5-(4-fluorophenyl)-4,5-dihydro-1H-pyrazol-1-yl)thiazole (14)

Yield 63%; m.p. 252-4°C; ¹H NMR (DMSO-d₆) δ 4.04–4.07 (dd, 2H, CH₂, J = 11.45 Hz, J = 10.33 Hz), 5.73 (t, 1H, CH, J = 5.35 Hz, J = 6.1 Hz), 6.88 (s, 2H, benzofuryl-H), 7.19–7.70 (m, 13H, Ar-H); MS m/z (%): 479 (M⁺, 100).

3.1.10. 3-(2-(3-(Benzofuran-2-yl)-5-(4-fluorophenyl)-4,5-dihydro-1H-pyrazol-1-yl)thiazol-4-yl)-2H-chromen-2-one (15)

Yield 74%; m.p. 233-4°C; ¹H NMR (DMSO-d₆) δ 4.05, 4.08 (dd, 2H, CH₂, J = 11.45 Hz, J = 10.33 Hz), 5.72 (t, 1H, CH, J = 5.35 Hz, J = 6.1 Hz), 7.22–7.72 (m, 14H, Ar-H), 8.23 (s, 1H, coumarinyl-H); MS m/z (%): 507 (M⁺, 100).

3.1.11. 2-(3-(Benzofuran-2-yl)-5-(4-fluorophenyl)-4,5-dihydro-1H-pyrazol-1-yl)thiazolo[5,4-b]quinoxaline (16)

Yield 74%; m.p. 256-8°C; ¹H NMR (DMSO-d₆) δ 4.02, 4.06 (dd, 2H, CH₂, J = 11.45 Hz, J = 10.33 Hz), 5.70 (t, 1H, CH, J = 5.35 Hz, J = 6.1 Hz), 7.22–7.72 (m, 13H, Ar-H); MS m/z (%): 465 (M⁺, 100).

3.2. Antimicrobial Activity

Chemical compounds were individually tested against a panel of Gram-positive and Gram-negative bacterial pathogens and yeast. Antimicrobial tests were carried out by the agar well diffusion method [15] using 100 μL of suspension containing 1 × 10⁸ CFU/mL of pathological tested bacteria and 1 × 10⁶ CFU/mL of yeast spread on nutrient agar (NA) and Sabourund dextrose agar (SDA), respectively. After the media had cooled and solidified, wells (10 mm in diameter) were made in the solidified agar and loaded with 100 μL of tested compound solution prepared by dissolving 100 mg of the chemical compound in one mL of dimethyl sulfoxide (DMSO). The inculcated plates were then incubated for 24 h at 37°C for bacteria and at 28°C for yeast. Negative controls were prepared using DMSO employed for dissolving the tested compound. Ciprofloxacin (50 μg/mL) and ketoconazole (50 μg/mL) were used as standard for antibacterial and antifungal activities respectively. After incubation time, antimicrobial activity was evaluated by measuring the zone of inhibition against the test organisms and compared with that of the standard. The observed zone of inhibition is presented in Table 2. Antimicrobial activities were expressed as inhibition diameter zones in millimeters (mm). The experiment was carried out in triplicate, and the average zone of inhibition was calculated.

3.3. Minimal Inhibitory Concentration (MIC) Measurement

The bacteriostatic activity of the active compounds (having inhibition zones (IZ) ≥ 18 mm) was then evaluated using the twofold serial dilution technique [16]. Twofold serial dilutions of the tested compounds solutions were prepared using the proper nutrient broth. The final concentration of the solutions was 200, 100, 50, and 25 μg/mL. The tubes were then inoculated with the test organisms, grown in their suitable broth at 37°C for 24 hours for bacteria (about 1 × 10⁸ CFU/mL and 1 × 10⁶ CFU/mL of yeast), and each 5 mL received 0.1 mL of the above inoculum and incubated at 37°C for 24 hours. The lowest concentration showing no growth was taken as the minimum inhibitory concentration (MIC).

4. Conclusion

Novel pyrazolylthiazoles, with potential antimicrobial activity, were prepared from available 2-acetylbenzofuran. Firstly, chalcone 3 was obtained by condensation with 4-fluorobenzaldehyde in alcoholic NaOH. This precursor reacted with thiosemicarbazide in strong basic medium to afford the N-pyrazoline thioamide 5. Reaction of the latter with different haloketones and 2,3-dichloroquinoxaline gave the target pyrzolinylthiazoles 8–16. The new compounds were tested for their antimicrobial activities and significant activities due to presence of three nucleuses: benzofuran, pyrazole, and thiazole. Also, some substituent increases the antimicrobial activities such as chloro substituent in compounds 8c and 13a.

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