ISRN OncologyVolume 2012 (2012), Article ID 756591, 6 pagesdoi:10.5402/2012/756591

Review Article

Metastatic Squamous Cell Carcinoma of the Anus: Time for a Shift in the Treatment Paradigm?

Alice Dewdney and Sheela Rao

Department of Medicine, Royal Marsden Hospital, London and Surrey, UK

Received 10 January 2012; Accepted 29 January 2012

Academic Editor: H. Al-Ali

Copyright © 2012 Alice Dewdney and Sheela Rao. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract.

Anal cancers are rare tumours; however, the incidence is increasing in both men and women. Changing trends in sexual behaviour, smoking, and infection with the human papillomavirus are thought to be responsible for the increase. Patients with metastatic disease have a poor prognosis, with 5-year median overall survival rates of 10% in men and 20% in women. The standard systemic treatment of metastatic disease remains cisplatin and 5-fluorouracil, and aside from several non-randomised small phase II trials there has been no real progress over the past two decades. Based on the efficacy of cetuximab in squamous cell carcinomas from other primary sites, there appears to be clinical rationale for evaluation of anti-epidermal growth factor inhibitors in anal squamous cell carcinoma. In order to facilitate research and implement more effective treatment strategies international collaboration in clinical trials incorporating tissue collection for biomarkers is essential.

1. Introduction

Anal cancers are rare tumours; in the UK there are approximately 900–1000 cases diagnosed per year, and in the USA they account for 1-2% of all gastrointestinal cancers [1]. However, the incidence is increasing in certain populations with high-risk behaviour and in those with the human immunodeficiency virus (HIV) [2, 3]. One of the most well-known risk factors is the human papillomavirus (HPV), particularly HPV type 16, which is present in approximately 80% of patients diagnosed with anal cancer [4]. Other risk factors include smoking, increased number of sexual partners, sexually transmitted infections, a history of vaginal or cervical malignancy, other conditions associated with lowered immunity, for example, transplant recipients and those with anal inflammatory conditions. Eighty percent of anal cancers are squamous cell carcinomas (SCCs); other rarer types of histology include adenocarcinoma, basal cell carcinoma, and melanoma [5].

The majority of cases present with early-stage localized disease. In this setting combined modality treatment with chemoradiation remains the standard of care allowing sphincter sparing whilst reserving surgery for those with persistent or recurrent disease following treatment [6]. Based on the available data a radiation dose of at least 45–50 Gy in combination with MMC and 5FU is currently the most commonly employed regimen [7–10].

Early-stage disease (T1/T2 N0) is associated with a good prognosis [11]. However, the five-year overall survival for those with more locally advanced disease ranges from 40–80% and 10 to 20% of patients will develop distant metastases following combined modality treatment [10, 12]. The prognosis for patients with distant metastases is generally poor, although documented median survival rates vary from 8 to 34 months. The SEER database reports 5-year survival rates of 10% in men and 20% in women between 1973 and 2000 [2, 11].

This paper reviews the existing literature regarding systemic treatment of patients with metastatic squamous cell carcinoma of the anus and explores potential future directions.

2. Trials of Systemic Chemotherapy

It is striking that in anal cancer only six randomized trials have been performed over the last three decades. In the metastatic setting the available evidence is based on small phase II trials (Table 1), retrospective series, and case reports (Table 2), and there have been no prospective phase III studies or meta-analyses.

Table 1: Phase II chemotherapy trials of metastatic squamous cell carcinoma of the anus.

Author
Number of patients
Agents
Response rate
Median PFS (months)
OS (months)

Wilking et al. [13]
15
Vincristine, bleomycin, and high-dose methotrexate
3/12 (25%)
2
NR

Hainsworth et al. [14]
60 (7 with anal cancer)
Paclitaxel, carboplatin, and infusional 5FU
65% overall 4/7 (57%) anal patients
35 overall (26 anal patients)
NR

Jhawer et al. [15]
20
Mitomycin C, adriamycin, cisplatin, and bleomycin-CCNU
12/20 (60%)
8
15

Table 2: Case reports of single-agent chemotherapy in metastatic anal squamous cell carcinoma.

Author
Number of patients
Agent
Response
PFS/duration of response (months)
OS

Evans et al. [16]
1
Carboplatin
Partial
9
NR

Fisher et al. [17]
1
Doxorubicin and cis-dichlorodiammineplatinum
Major
NR
NR

Zimm and Wampler [18]
1
Semustine
Partial
15
NR

Golub et al. [19]
3
TIP
Complete (3/3)
4,6,36
NR

Grifalchi et al. [20]
1
Irinotecan
Partial
NR
NR

The most plausible explanation is simply the relative rarity of the disease and the small number of patients treated at each centre. Unfortunately anal cancer still suffers from a social stigma with an “embarrassing location” and the association with sexually transmitted infections and HIV. There has certainly not been the same degree of public interest and support for anal cancer as demonstrated in other solid tumours, for example, breast or testicular cancer.

Systemic chemotherapy remains the mainstay of treatment for patient with metastatic disease. The choice of chemotherapy is influenced by any previous treatment the patient received for early disease, the disease-free interval, and the patient’s performance status and wishes. The National Comprehensive Cancer Network (NCCN) Guidelines currently recommend cisplatin and 5FU chemotherapy as first-line treatment of metastatic squamous cell carcinoma (SCC) [21]. This is largely based on a study of 19 patients treated with cisplatin 100 mg/m² and infusional 5FU 1 gm/m²/day over 5 days with a 66% response rate; there was 1 complete response and 11 partial responses in addition to 4 patients with stable disease. The actuarial survival was 62.2% at 1 year and 32.2% at 5 years, and the median survival was 34.5 months [22]. There have also been a number of case reports demonstrating a benefit with the cisplatin/5FU combination [23–25].

Recently the oral fluoropyrimidine capecitabine has been substituted for 5FU in a number of solid tumours, and, extrapolating from this data, many centres now use capecitabine with cisplatin rather than 5FU [26]. This has a number of advantages for patients, including ease of administration and avoidance of indwelling lines and their associated complications [27].

MMC and 5FU are an alternative first line combination although there is less evidence to suggest an impact on survival. In a retrospective series, three of eight patients treated with MMC and 5FU had a response to treatment in terms of decrease in tumour size, reduction in pain, and improvement in performance status [28].

In an early phase II study, vincristine, bleomycin, and high-dose methotrexate were given to 15 patients; three out of the twelve patients with measurable disease (25%) had objective responses of 1-, 2-, and 5-month duration. However five of the fifteen patients had severe or life-threatening complications as a result of this treatment regimen and subsequently the regimen was deemed too toxic to warrant further evaluation [13].

In a single arm trial of twenty patients treated with mitomycin C, adriamycin, cisplatin, and bleomycin-CCNU, 12 (60%) patients had a partial response and no complete responses were observed. The median survival in this study was 15 months (95% CI 6–20 months), and the median time to progression or death was 8 months (95% CI 4–9 months). Toxicity was not insignificant, and although these side effects were managed without sequelae, the combination has not been explored in a phase III trial due to toxicity [15].

The most encouraging results have been demonstrated in a phase II single arm study of paclitaxel, carboplatin, and continuous infusional 5FU in patients with advanced squamous carcinomas () from multiple primary sites, given first or second line. Seven patients with SCC of the anus were included, of these four responded, two of whom had a complete response. The patients with a complete response then received consolidation radiotherapy to the primary tumour. The median duration of response in the anal subgroup was 26 months (range 10–63+) [14]. Although most patients were able to tolerate full doses of all three drugs, gastrointestinal toxicities (mucositis and diarrhoea) were responsible for most dose reductions. Interestingly substantial activity of this three-drug combination was demonstrated across all tumour types in the trial, with an overall complete response rate of 25%.

There have been several single case reports or series evaluating alternative monotherapy or combination chemotherapy regimens. These data are limited by relatively small numbers and lack of prospective validation. The three-drug regimen, paclitaxel, ifosfamide and cisplatin (TIP), has been used in head and neck cancer and cervical squamous cell carcinomas, and a recent case series reported three patients with metastatic anal SCC treated with TIP. All three patients had a complete response with disease-free intervals of 4, 6, and 36 months, all required prophylactic GCSF support during their treatment [19].

3. Localised Therapies

The liver is the most common site of metastases, and the role of regional therapy has been explored in a multicentre analysis of 52 patients with squamous cell carcinomas, 27 of whom had metastatic anal cancer. Patients underwent hepatic resection (), radiofrequency ablation (), or both (), and the majority had received systemic therapy prior to resection. When the analysis was restricted to the 27 patients with metastatic anal cancer, the median disease-free and overall survival durations were 9.6 and 22.3 months, respectively [32]. These data suggest that there may be a subset of patients with isolated hepatic metastases who may benefit from resection, but selection criteria are as yet undefined and metastasectomy is not currently standard practice.

Palliative surgery for the primary lesion in those with metastatic disease and symptomatic local disease is an option. For those with significant comorbidities unable to tolerate systemic chemotherapy or surgery palliative radiotherapy alone is an alternative local therapy. Effective palliation can be achieved with a dose of 45–54 Gy in 25–30 fractions; however, alternative shorter or hypofractionated regimens such as 30 Gy in 10 fractions or 6 Gy/week for 5-6 weeks may be more appropriate for frailer patients.

The role of a shortened course of chemoradiation (30 Gy in 10 fractions with concurrent 5FU) has also been evaluated in elderly patients, demonstrating high rates of local control [33].

4. The Role of EGFR-Targeted Therapies in Anal Cancer

Squamous cell carcinoma of the anal canal has a number of similarities to cervical and head and neck squamous tumours, in terms of histology, a strong causative association with HPV infection and relative radiosensitivity. Squamous cell carcinoma of the anus, in common with cervical and head and neck squamous tumours, regularly overexpresses the epidermal growth factor receptor (EGFR). In a large cohort of invasive anal SCC tissue samples () immunohistochemistry analysis of EGFR demonstrated that the majority of invasive anal SCCs overexpressed EGFR. Of the 101 patient biopsies available, 82 samples had sufficient material for interpretation of these 72/82 (90%) stained positive for EGFR, while 41/82 (50%) samples displayed at least moderate to strong staining [34]. Similar results have been demonstrated in a number of smaller studies, with range of EGFR overexpression of 55–100% [35, 36]. Interestingly EGFR gene amplification was not observed in these studies suggesting that overexpression occurs via mechanisms other than gene amplification.

There are data to suggest a correlation between oncogenic HPV and EGFR expression. In a study by Walker et al. 96% of invasive HPV-infected anal SCCs displayed strong membrane immunoreactivity to EGFR expression. It is also thought that HIV-positive status contributes in augmenting EGFR expression levels that are involved in carcinogenesis [37].

Mutations in the downstream effectors KRAS and BRAF, which are responsible for a lack of response to anti-EGFR antibodies in colorectal adenocarcinomas, appear rarer in anal tumours [38]. In a study of 95 tumour biopsies from squamous cell anal cancers, the tumours lacked the common KRAS and EGFR mutations. Only three samples had an EGFR exon 21 mutation and none had KRAS mutations. In a smaller series of three tumours that expressed EGFR, none had a mutation in KRAS codon 12 or 13 [39]. The anti-EGFR monoclonal antibody cetuximab has demonstrated efficacy in head and neck squamous cell tumours when administered concomitantly with radiotherapy [40]. In a case series of 7 heavily pretreated anal cancer patients, cetuximab was given in combination with irinotecan. In contrast to previous studies, KRAS mutations were demonstrated in a number of patients in this case series. Response (defined as partial or minor) was seen in the KRAS wild type patients () with no response demonstrated in the patients with KRAS mutations () [30]. These findings correlate with the results of two case reports of patients who achieved an excellent response to treatment with irinotecan and cetuximab having previously failed irinotecan [29, 31] (Table 3).

Table 3: Studies of cetuximab in metastatic anal squamous cell carcinoma.

Author
Number of patients
Agent
Response
PFS/duration of response
OS

De Dosso et al. [29]
1
Irinotecan + cetuximab
Partial
Patient died of PE on treatment
NR

Lukan et al. [30]
7
Irinotecan + cetuximab
5/7 response* (3 partial, 1 minor, and 1 stable)
6
NR

Phan and Hoff [31]
1
Irinotecan + cetuximab
Partial
NR
NR

* All five that responded were KRAS wild type.

These data in a relatively small patient population and the presence of EGFR overexpression in anal SCC suggest that the combination of irinotecan and cetuximab warrants further evaluation. An alternative combination of paclitaxel with cetuximab has been evaluated in a number of squamous cell carcinomas with encouraging results. In a phase II trial of patients with stage IV non-small-cell lung cancer (NSCLC), a 3-weekly combination of paclitaxel (100 mg/m²/week for 3 weeks), carboplatin (area under curve = 6), and cetuximab (400 mg/m² loading dose followed by 250 mg/m²/week) was safe and well tolerated; the response rate was 57% with an overall survival of 13.8 months [41]. A weekly regimen of paclitaxel (80 mg/m²/week) with cetuximab (400 mg/m² loading dose followed by 250 mg/m²/week) was evaluated in a phase II study in squamous cell carcinoma of the head and neck; the combination was well tolerated with an overall response rate of 71% and disease control rate of 88% [42]. In light of similarities in the histology and pathogenesis between squamous cell carcinomas, these results suggest that the combination of paclitaxel and cetuximab is worthy of exploration in anal cancer.

5. Prognostic Factors

Poor prognostic factors include male sex, age over 65 years, more advanced T stage, nodal involvement, or poorly differentiated histology. In a review of prognostic factors derived from a prospective database male sex and clinically node positive disease were confirmed as poor prognostic factors for both disease-free and overall survival [43]. Black African Americans have worse outcomes than white American patients. Anal cancer is not an AIDS-defining illness; however, patients with HIV appear to have lower overall survival rates compared to HIV-negative patients [44].

A number of potential prognostic biomarkers have been evaluated in small studies; the tumour suppressor genes p53 and p21 are the only markers found to have an association with outcome in more than one study [45]. Even these results are not consistent; p53 was associated with worse outcome in two studies but no association was demonstrated in six others [46–48]. Variability in methodology and small patient numbers may explain the discordance results [49, 50]. Alternative potential markers including apoptotic regulators (Bcl2 BAX, and NF-KB), EGFR, cyclins A, D, and E, markers of proliferation (ki67, and MiBL), and angiogenic factors have also been evaluated in small studies. None of the results have been replicated in larger studies, and as yet there is no biomarker with the ability to predict outcome in this disease.

6. Conclusions

There have been very few developments in the treatment of metastatic disease for some time and currently there are no ongoing phase II or III trials specifically for anal cancer. In the era of Personalized medicine it would appear time for a shift in the treatment paradigm for patients with metastatic anal cancer. New experimental regimens may achieve better results and novel strategies of combination cytotoxic platforms with anti-EGFR targeted agent may lead to improved efficacy. In order to facilitate research and implement more effective treatment strategies clinical trials incorporating tissue collection for biomarkers are essential. International collaboration in global clinical trials is imperative and could improve outcomes in this setting.

Acknowledgments

The authors acknowledge NHS funding from the NIHR Biomedical Research Centre and the Peter Stebbings Memorial Charity.

References

http://www.fascrs.org/patients/conditions/anal_cancer/

L. G. Johnson, M. M. Madeleine, L. M. Newcomer, S. M. Schwartz, and J. R. Daling, “Anal cancer incidence and survival: the Surveilance, epidemiology, and end results experience, 1973–2000,” Cancer, vol. 101, no. 2, pp. 281–288, 2004.

N. F. Crum-Cianflone, K. H. Hullsiek, V. C. Marconi et al., “Anal cancers among HIV-infected persons: HAART is not slowing rising incidence,” AIDS, vol. 24, no. 4, pp. 535–543, 2010.

J. R. Daling, M. M. Madeleine, L. G. Johnson et al., “Human papillomavirus, smoking, and sexual practices in the etiology of anal cancer,” Cancer, vol. 101, no. 2, pp. 270–280, 2004.

D. P. Ryan and R. J. Mayer, “Anal carcinoma: histology, staging, epidemiology, treatment,” Current Opinion in Oncology, vol. 12, no. 4, pp. 345–352, 2000.

http://www.nccn.org/professionals/physician_gls/pdf/anal.pdf

B. Sischy, R. L. Scotte Doggett, J. M. Krall et al., “Definitive irradiation and chemotherapy for radiosensitization in management of anal carcinoma: interim report on Radiation Therapy Oncology Group Study No. 8314,” Journal of the National Cancer Institute, vol. 81, no. 11, pp. 850–856, 1989.

J. Northover, R. Glynne-Jones, D. Sebag-Montefiore et al., “Chemoradiation for the treatment of epidermoid anal cancer: 13-year follow-up of the first randomised UKCCCR Anal Cancer Trial (ACT I),” British Journal of Cancer, vol. 102, no. 7, pp. 1123–1128, 2010.

R. James, S. Wan, R. Glynne-Jones, et al., “A randomized trial of chemoradiation using mitomycin or cisplatin, with or without maintenance cisplatin/5FU in squamous cell carcinoma of the anus (ACT II),” Journal of Clinical Oncology, vol. 27, no. 18s, supplement, abstract LBA4009, 2009.

H. Bartelink, F. Roelofsen, F. Eschwege et al., “Concomitant radiotherapy and chemotherapy is superior to radiotherapy alone in the treatment of locally advanced anal cancer: results of a phase III randomized trial of the European organization for research and treatment of cancer radiotherapy and gastrointestinal cooperative groups,” Journal of Clinical Oncology, vol. 15, no. 5, pp. 2040–2049, 1997.

http://seer.cancer.gov/publications/survival/surv_anus.pdf

J. M. A. Northover, S. J. Arnott, D. Cunningham et al., “Epidermoid anal cancer: results from the UKCCCR randomised trial of radiotherapy alone versus radiotherapy, 5-fluorouracil, and mitomycin,” Lancet, vol. 348, no. 9034, pp. 1049–1054, 1996.

N. Wilking, N. Petrelli, L. Herrera, and A. Mittelman, “Phase II study of combination bleomycin, vincristine and high-dose methotrexate (BOM) with leucovorin rescue in advanced squamous cell carcinoma of the anal canal,” Cancer Chemotherapy and Pharmacology, vol. 15, no. 3, pp. 300–302, 1985.

J. D. Hainsworth, H. A. Burris III, A. A. Meluch, M. N. Baker, L. H. Morrissey, and F. Anthony Greco, “Paclitaxel, carboplatin, and long-term continuous infusion of 5-fluorouracil in the treatment of advanced squamous and other selected carcinomas: results of a Phase II trial,” Cancer, vol. 92, no. 3, pp. 642–649, 2001.

M. Jhawer, S. Mani, M. Lefkopoulou et al., “Phase II study of mitomycin-C, adriamycin, cisplatin (MAP) and Bleomycin-CCNU in patients with advanced cancer of the anal canal: an eastern cooperative oncology group study E7282,” Investigational New Drugs, vol. 24, no. 5, pp. 447–454, 2006.

T. R. J. Evans, J. L. Mansi, and J. P. Glees, “Response of metastatic anal carcinoma to single agent carboplatin,” Clinical Oncology, vol. 5, no. 1, pp. 57–58, 1993.

W. B. Fisher, K. D. Herbst, J. E. Sims, and C. F. Critchfield, “Metastatic cloacogenic carcinoma of the anus: sequential responses to adriamycin and cis-dichlorodiammineplatinum(II),” Cancer Treatment Reports, vol. 62, no. 1, pp. 91–97, 1978.

S. Zimm and L. Wampler, “Response of metastatic cloacogenic carcinoma to treatment with semustine,” Cancer, vol. 48, no. 12, pp. 2575–2576, 1981.

D. V. Golub, A. C. Civelek, and V. R. Sharma, “Case report; a regimen of taxol, ifosfamide, and platinum for recurrent advanced squamous cell cancer of the anal canal,” Chemotherapy Research and Practice, vol. 2011, Article ID 163736, 6 pages, 2011.

F. Grifalchi, A. Padovani, F. Romeo, C. Trinca, L. Moscetti, and E. Cortesi, “Response of metastatic epidermoid anal cancer to single agent irinotecan: a case report,” Tumori, vol. 87, no. 1, pp. 58–59, 2001.

http://www.nccn.org/professionals/physician_gls/f_guidelines.asp

C. Faivre, P. Rougier, M. Ducreux et al., “5-Fluorouracile and cisplatinum combination chemotherapy for metastatic squamous-cell anal cancer,” Bulletin du Cancer, vol. 86, no. 10, pp. 861–865, 1999.

R. Khater, M. Frenay, and J. Bourry, “Cisplatin plus 5-fluorouracil in the treatment of metastatic anal squamous cell carcinoma: a report of two cases,” Cancer Treatment Reports, vol. 70, no. 11, pp. 1345–1346, 1986.

J. A. Ajani, C. H. Carrasco, D. E. Jackson, and S. Wallace, “Combination of cisplatin plus fluoropyrimidine chemotherapy effective against liver metastases from carcinoma of the anal canal,” American Journal of Medicine, vol. 87, no. 2, pp. 221–224, 1989.

I. A. Jaiyesimi and R. Pazdur, “Cisplatin and 5-fluorouracil as salvage therapy for recurrent metastatic squamous cell carcinoma of the anal canal,” American Journal of Clinical Oncology, vol. 16, no. 6, pp. 536–540, 1993.

D. Cunningham, N. Starling, S. Rao et al., “Capecitabine and oxaliplatin for advanced esophagogastric cancer,” New England Journal of Medicine, vol. 358, no. 1, pp. 36–46, 2008.

P. Pfeiffer, J. P. Mortensen, B. Bjerregaard et al., “Patient preference for oral or intravenous chemotherapy: a randomised cross-over trial comparing capecitabine and Nordic fluorouracil/leucovorin in patients with colorectal cancer,” European Journal of Cancer, vol. 42, no. 16, pp. 2738–2743, 2006.

M. J. Greenall, G. B. Magill, S. H. Q. Quan, and J. J. DeCosse, “Recurrent epidermoid cancer of the anus,” Cancer, vol. 57, no. 7, pp. 1437–1441, 1986.

S. De Dosso, V. Martin, E. Zanellato, M. Frattini, and P. Saletti, “Molecular characterization and response to cetuximab in a patient with refractory squamous cell anal carcinoma,” Tumori, vol. 96, no. 4, pp. 627–628, 2010.

N. Lukan, P. Ströbel, A. Willer et al., “Cetuximab-based treatment of metastatic anal cancer: correlation of response with KRAS mutational status,” Oncology, vol. 77, no. 5, pp. 293–299, 2009.

L. K. Phan and P. M. Hoff, “Evidence of clinical activity for cetuximab combined with irinotecan in a patient with refractory anal canal squamous-cell carcinoma: report of a case,” Diseases of the Colon and Rectum, vol. 50, no. 3, pp. 395–398, 2007.

T. M. Pawlik, A. L. Gleisner, T. W. Bauer et al., “Liver-directed surgery for metastatic squamous cell carcinoma to the liver: results of a multi-center analysis,” Annals of Surgical Oncology, vol. 14, no. 10, pp. 2807–2816, 2007.

N. Charnley, A. Choudhury, P. Chesser, R. A. Cooper, and D. Sebag-Montefiore, “Effective treatment of anal cancer in the elderly with low-dose chemoradiotherapy,” British Journal of Cancer, vol. 92, no. 7, pp. 1221–1225, 2005.

A. Paliga, R. Onerheim, A Gologan, et al., “EGFR expression in invasive anal carcinoma,” Journal of Clinical Oncology, vol. 29, no. 4, supplement, abstract 412, 2011.

L. H. Lê, R. Chetty, and M. J. Moore, “Epidermal growth factor receptor expression in anal canal carcinoma,” American Journal of Clinical Pathology, vol. 124, no. 1, pp. 20–23, 2005.

G. Alvarez, A. Perry, B. R. Tan, and H. L. Wang, “Expression of epidermal growth factor receptor in squamous cell carcinomas of the anal canal is independent of gene amplification,” Modern Pathology, vol. 19, no. 7, pp. 942–949, 2006.

F. Walker, L. Abramowitz, D. Benabderrahmane et al., “Growth factor receptor expression in anal squamous lesions: modifications associated with oncogenic human papillomavirus and human immunodeficiency virus,” Human Pathology, vol. 40, no. 11, pp. 1517–1527, 2009.

A. Paliga, G. Chong, R. Onerheim, et al., “KRAS and EGFR gene mutation status in anal canal carcinoma,” in Proceedings of the Gastrointestinal Cancers Symposium, Abstract 352, 2010.

M. W. Saif, E. Kontny, K. N. Syrigos, and A. Shahrokni, “The role of EGFR inhibitors in the treatment of metastatic anal canal carcinoma: a case series,” Journal of Oncology, vol. 2011, Article ID 125467, 5 pages, 2011.

J. A. Bonner, P. M. Harari, J. Giralt et al., “Radiotherapy plus cetuximab for squamous-cell carcinoma of the head and neck,” New England Journal of Medicine, vol. 354, no. 6, pp. 567–578, 2006.

H. Borghaei, C. J. Langer, M. Millenson et al., “Phase II study of paclitaxel, carboplatin, and cetuximab as first line treatment, for patients with advanced non-small cell lung cancer (NSCLC): results of OPN-017,” Journal of Thoracic Oncology, vol. 3, no. 11, pp. 1286–1292, 2008.

R. Hitt, et al., “Phase II study of the combination of cetuximab and weekly paclitaxel in the first-line treatment of patients with recurrent and/or metastatic squamous cell carcinoma of head and neck,” Annals of Oncology. In press

J. A. Ajani, K. A. Winter, L. L. Gunderson et al., “Prognostic factors derived from a prospective database dictate clinical biology of anal cancer: the intergroup trial (RTOG 98-11),” Cancer, vol. 116, no. 17, pp. 4007–4013, 2010.

S. Edelman and P. A. S. Johnstone, “Combined modality therapy for HIV-infected patients with squamous cell carcinoma of the anus: outcomes and toxicities,” International Journal of Radiation Oncology Biology Physics, vol. 66, no. 1, pp. 206–211, 2006.

T. Lampejo, D. Kavanagh, J. Clark et al., “Prognostic biomarkers in squamous cell carcinoma of the anus: a systematic review,” British Journal of Cancer, vol. 103, no. 12, pp. 1858–1869, 2010.

R. Holm, E. Skovlund, H. Skomedal, V. A. Flørenes, and G. Tanum, “Reduced expression of p21WAF1 is an indicator of malignant behaviour in anal carcinomas,” Histopathology, vol. 39, no. 1, pp. 43–49, 2001.

C. S. Wong, M. S. Tsao, V. Sharma, W. B. Chapman, M. Pintilie, and B. J. Cummings, “Prognostic role of p53 protein expression in epidermoid carcinoma of the anal canal,” International Journal of Radiation Oncology Biology Physics, vol. 45, no. 2, pp. 309–314, 1999.

S. R. Bonin, T. F. Pajak, A. H. Russell et al., “Overexpression of p53 protein and outcome of patients treated with chemoradiation for carcinoma of the anal canal: a report of randomized trial RTOG 87-04,” Cancer, vol. 85, no. 6, pp. 1226–1233, 1999.

J. A. Ajani, X. Wang, J. G. Izzo et al., “Molecular biomarkers correlate with disease-free survival in patients with anal canal carcinoma treated with chemoradiation,” Digestive Diseases and Sciences, vol. 55, no. 4, pp. 1098–1105, 2010.

N. A. Shepherd, J. H. Schofefield, S. B. Love, J. England, and J. M. A. Northover, “Prognositc factors in anal squamous carcinoma: a multivariate analysis of clincal, pathological and flow cytometric parameters in 235 cases,” Histopathology, vol. 16, no. 6, pp. 545–555, 1990.