ISRN CardiologyVolume 2013 (2013), Article ID 858921, 6 pageshttp://dx.doi.org/10.1155/2013/858921

Clinical Study

Assessment of Risk Factor for Cardiovascular Disease Using Heart Rate Variability in Postmenopausal Women: A Comparative Study between Urban and Rural Indian Women

Nikhil Narayanaswamy,¹ Shailaja Moodithaya,² Harsha Halahalli,² and Amrit M. Mirajkar²

¹K S Hegde Medical Academy, Nitte University, Mangalore 575 018, India²Department of Physiology, K S Hegde Medical Academy, Nitte University, Mangalore 575 018, India

Received 28 May 2013; Accepted 18 June 2013

Academic Editors: E. Liehn and G. Piccirillo

Copyright © 2013 Nikhil Narayanaswamy et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract.

Cardiovascular diseases are important causes of morbidity and mortality in postmenopausal women. A major determinant of cardiovascular health is the status of autonomic nervous system and assessment of Heart Rate Variability (HRV). Heart Rate Variability is a noninvasive and sensitive technique to evaluate cardiovascular autonomic control. Reduced HRV is an independent risk factor for the development of heart disease. This study evaluated the risk factors for cardiovascular diseases using HRV, between urban and rural Indian postmenopausal women ranging in age from 40 to 75 years. Findings of the analysis of HRV have showed that the total power which reflects overall modulation of cardiac autonomic activity (  versus ), the absolute power of high frequency which is surrogate of cardiovagal activity (  versus ), and low frequency that reflects cardiac sympathetic activity (  versus ) were significantly higher in urban women than that of their rural counterparts. Further, among the anthropometric measures, waist circumference was significantly correlated with indices of HRV. The study concludes that rural Indian women are associated with an additional risk beyond that of ageing and postmenopausal status when compared to the urban women. The higher central obesity could be the contributing factor for developing higher risk for cardiovascular disease among the rural women.

1. Introduction

As women age, their health is influenced by factors such as career, diet, physical activity level, the socioeconomic status, and environment [1]. These changes, together with natural menopause, process of ageing, and hormonal changes in the reproductive system, affect the well-being of women. The complex, interrelated nature of the process often makes it difficult to distinguish between the symptoms of ageing or those resulting from the loss of ovarian functions, and factors arising out of socioenvironmental conditions. The differences in socioeconomic, nutritional status and physical activity level between the urban and rural population might influence postmenopausal women health more significantly than the hormonal changes.

Menopausal status is accompanied by unfavorable levels of cardiovascular risk factors, like changes in body fat, distribution from gynoid to android pattern, abnormal plasma lipids, increased sympathetic tone, endothelial dysfunction, vascular inflammation, and increased blood pressure [2]. A major determinant of cardiovascular health and prognosis is the status of autonomic nervous system [3], although ignored by many clinicians. Abnormal autonomic control of heart rate is associated with an increased risk of cardiovascular morbidity and mortality [4]. The periodic fluctuations of heart rate are indicative of the relative contributions of sympathetic and parasympathetic components of autonomic nervous system to the heart and reduction of Heart Rate Variability (HRV) has been reported in several cardiac and noncardiac diseases.

Ethnicity, gender, and geography are powerful modifiers of community health. It is possible that geography is more powerful than any risk factor yet to be discovered. Geographic concentration of disease burden may have many causes including inadequate health care infrastructure, high level of poverty, and remote location [5]. Urban environment is associated with increased opportunities for mechanized or sedentary employment, consumption of processed foods, and other lifestyle characteristics associated with the development of cardiovascular diseases. A study also reported that rural to urban migration augments sedentary life style and undesirable eating habits among both men and women and thus accelerates the development of risk for diseases [6]. It has been shown that over the past fifty years prevalence of obesity, hypertension, hypercholesterolemia, and diabetes has increased significantly in urban and slowly in rural areas [7, 8]. Studies have shown that there is a greater incidence of cardiac disease among urban postmenopausal women due to the fact that they lead a sedentary lifestyle, while their rural counterparts are accustomed to greater amount of physical activity. However, another study comparing urban and rural women in United States for cardiovascular disease risk factors showed that the rural United States women had lower levels all types of HDL which is considered the protective cholesterol than that of urban United States women [9].

In the Indian scenario, the public health care system has typically concentrated on women of childbearing age, and once the women move out of this bracket they receive less attention unless they have access to private health care [3]. Menopausal health research also demands priority in Indian setting due to increase in life expectancy as a result of which women normally live between 10 and 20 percent of their lives in the postmenopausal state [10]. The United States Preventive Service Task Force has identified clear evidence that the health of women can be improved through preventive health screening [2].

This study attempted to evaluate HRV as risk factor for cardiovascular in postmenopausal women health contrasted between urban and rural women. The study also attempted to evaluate the association of indices of HRV with anthropometric measures among postmenopausal women. By categorizing postmenopausal women as high risk for cardiovascular diseases, findings of this study would play an important role in managing the disease, by early identification using cardiac autonomic function as a factor. The study hypothesized that rural postmenopausal women have higher heart rate variability, therefore lower degree of risk for cardiovascular disease as compared to urban women.

2. Materials and Methods

2.1. Subjects

A total of 60 postmenopausal women were recruited: 30 from urban and 30 from rural population based on location of their residence in South India. Subjects were recruited after taking a thorough clinical history. Women aged between 40 and 75 years, with absence of menstrual cycles for at least one year, were included. Subjects who had undergone hysterectomy, with history of chronic illness and on hormone replacement therapy, were excluded from the study. Informed written consent was obtained from all participants, and the experiment protocol was approved by Ethics committee of the institute.

2.2. Experimental Protocol

All the participants reported for the study, after refraining from food, caffeinated or cocoa containing beverages for at least 2 hours. They were also instructed not to consume alcohol or tobacco 12 hours prior to recording. All the subjects underwent measurement of height, weight, and basal blood pressure. Height was measured to the nearest 0.1 cm without footwear, using vertically movable scale. Weight was measured to the nearest 100 grams using a digital scale. A basal recording of BP was done using Automatic Blood Pressure Monitor (Model SEM-2 Electronic Instrument, Omron Technologies). Subjects were also underwent the measurement of waist and hip circumstances. Waist circumferences were measured at the midpoint between the lower rib cage and iliac crest. Hip circumferences were measured at maximum circumference by placing the tape around the buttocks, without compressing the skin. Using the waist and hip circumferences the waist-hip ratio was calculated.

HRV was assessed based on the RR intervals at resting condition. To quantify RR intervals, the analog ECG signal was recorded using lead II, to obtain a QRS complex of sufficient amplitude and stable baseline. ECG signals were conveyed through an A/D converter (after ECT Monitoring System, Niviqure Meditech Pvt. Ltd., Bangalore). The system has an integrated software which allows for recording of the ECG at a sampling frequency of 1024 Hz, with sampling rate of 8/s. 3 M standard chest electrodes (Ag-AgCl) were used. 4 electrodes were placed on the chest wall, one each on either side of the clavicle on the mid clavicular line and one each on the outermost and lowermost ribs.

ECG was recorded for a period of 5 minutes during which the subject was supine, awake resting, and normally breathing. Subjects were asked to avoid unnecessary movements during this period. The data so gathered was subjected to spectral analysis in the following way.

2.3. HRV Analysis

A noise-free electrocardiogram was obtained with the sampling frequency of 1024 Hz. Spectral analysis was performed off-line using Physionet. Data was edited manually for artifacts. HRV software uses a peak detection algorithm to find the “R” wave. The detection was done at a resampling rate of 4 Hz. Each detected “R” wave was considered as a data point. A minimum of 256 data points are required to perform a spectral analysis, for which a minimum duration of 5 minutes of ECG recording was obtained.

Spectral analysis is performed using a Fast Fourier Transform (FFT). The power is calculated in two bands. The 0.15-0.4 Hz band of RR power (high frequency) reflects parasympathetic nerve activity to the heart, while 0.04-0.15 Hz (low frequency band) is believed to reflect, at least in part, sympathetic nerve activity to the heart. In addition to absolute power, the data is also presented as normalized units and ratio of low frequency to high frequency (LF/HF) represents a measure of the balance of sympathetic and parasympathetic function.

2.4. Statistical Analysis

The analysis has been performed using SPSS 17.0 package. Data is expressed as mean ± SE. Values have been approximated to the third decimal. The total study population of 60 comprises of 2 independent groups with their respective data values. For the analysis of skewed data Mann-Whitney test has been used. Linear associations between the different HRV indices and anthropometric measures were tested using Pearson’s correlations. Differences were considered statistically significant at .

3. Results

See Tables 1, 2, and 3 and Figure 1.

Table 1: Subject characteristics.

Parameter
Urban ()
Rural ()

Age (years)

Height (m)

Weight (kg)

*

Body mass index (kg/m²)

Waist circumferences (cm)

*

Waist Hip ratio

Basal SBP (mm of Hg)

Basal DBP (mm of Hg)

Resting HR (min⁻¹)

Data expressed as mean ± SE.

*Significantly different across the two groups

SBP: systolic blood pressure; DBP: diastolic blood pressure; HR: heart rate.

Table 2: Comparison of HRV between urban and rural postmenopausal women.

Variables
Urban ()
Rural ()
value

Total power (ms²)

0.000*

High frequency (absolute in ms²)

0.010*

Low frequency (absolute in ms²)

0.015*

High frequency (normalized)

0.897

Low frequency (normalized)

0.897

LF/HF

0.885

Data expressed as mean ± SE.

*Significantly different across the two groups.

Table 3: Correlation matrix between HRV indices and anthropometric measures.

Parameter
Total power
HF ab
LF ab
LF/HF

Weight (kg)
−0.122
−0.170
−0.135
−0.137

BMI (kg/m²)
−0.122
−0.176
−0.206
−0.202

Waist/Hip
−0.169
−0.116
−0.206
−0.108

Waist circumference
−0.226
−0.281*
−0.302**
−0.122

Significant correlation.

**Highly significant correlation.

Figure 1: Comparison of HRV between urban and rural postmenopausal women. *Significant difference between the groups.

Figures 2 and 3 depict the format of result generated through Physionet.

(a)

(b)

Figure 2: Heart Rate Variability Report 1. Heart rate tachogram and RR interval power spectrum from an urban subject. Greater area of the graph falls under high frequency band.

(a)

(b)

Figure 3: Heart Rate Variability Report 2. Heart rate tachogram and RR interval power spectrum from a rural subject. Lesser area of the graph falls under high frequency band as compared to Figure 2.

4. Discussion

The present study hypothesized that the rural women would have lower risk for cardiac diseases since the idealized view of rural life is more active, least stressful, with healthy food habits, and greater social and community support. Therefore, the study compared the risk for cardio vascular disease by assessing the spectral analysis of Heart Rate Variability of thirty urban and rural postmenopausal women each.

Various techniques and maneuvers have been developed to detect the integrity of the sympathetic and parasympathetic nervous system. Most of the techniques such as cold pressor test, Valsalva maneuver, and the tilting table tests have focused on the evoked response of autonomic nervous system [11]. The evoked activities, however, might not reflect the tonic state of the body. Besides being a noninvasive study procedure, an important advantage of frequency-domain analysis of HRV is that it utilizes spontaneous fluctuation in heart rate to estimate tonic autonomic nervous functions which is sensitive index of cardiac morbidity.

In this study, the participants of the two study groups were age-matched, and urban women were significantly overweight compared to the rural women. However, the waist circumference which is surrogate of abdominal obesity was significantly higher among rural women compared to that of urban women. The basal systolic, diastolic blood pressure, and resting heart rate were comparable between the study groups (Table 1). Table 2 provides HRV indices among urban and rural women; the significant decreased total power of Heart Rate Variability among rural women indicates that they are associated with reduced overall cardiac autonomic modulation compared to that of urban postmenopausal women. Table 3 provides a correlation matrix between HRV indices and anthropometric measures. The findings of the study show that absolute HF spectrum and LF spectrum significantly correlate with waist circumference, which is a surrogate of central obesity, but not with weight, BMI, and waist hip circumferences.

Reduced HRV has been reported to be an independent risk factor for the development of coronary heart disease, cardiac sudden death, and all-cause mortality in women [12]. Therefore, the findings of this study indicate that rural postmenopausal women are associated with an additional risk beyond that of ageing and postmenopausal status when compared to urban women. Decreased high frequency component of the total variability in rural women observed, in this study, reflects the reduced overall protection of cardiac vagal modulation against cardiac mortality.

The observation of the present study is not in agreement with the study hypothesis. However, the findings of this study are comparable with the results of Broda et al. in which they reported that rural United States women had lower levels of all types of HDL which is considered to be the protective cholesterol, than that of urban women [9], which might explain to some extent our findings that rural women are at greater risk for CVD. Although studies reveal a 2-3-fold increase in the incidence of CHD among urban population than compared to rural population, they indicate a higher incidence of risk factors among the latter [13–15]. Chief among these are the increased calorie intake and greater percentage of saturated fat in diet consumed by the rural population, leading to increase in triglyceride to HDL cholesterol ratio, which has been described as an independent risk factor for CHD [13, 14]. Another factor which has not been taken into consideration in the present study is occupation. Our rural study population represents a region where majority of women are occupied with beedi rolling (hand rolled small cigarettes filled with tobacco) who handle about 35–40 kg of tobacco per month [16]. Studies have shown that a nearly 2-fold greater odds of CHD was found among women who were exposed to tobacco at work [17]. Significant correlation of HRV indices with waist circumference observed in this study is comparable with the report of Hagey and Warren, in which they report that the levels of central adiposity, which increase with age, contribute to the development of cardiovascular disease [18]. The causes underlying this geographical disparity in risk factors for cardiac disease among rural postmenopausal women could be inadequacy of healthcare infrastructure and the concomitant problems. A well designed and delivered rural health literacy program, addressing the health issues of elderly rural women, can motivate and bring in behavioral modifications necessary for good cardiovascular health.

5. Conclusion

Indian rural postmenopausal women are associated with an additional risk for cardiovascular disease beyond that of ageing and postmenopausal status when compared to urban women. The causes for underlying this geographical disparity in HRV could be due to different central adiposities between the groups. Exercise intervention for postmenopausal women should aim to improve pattern of fat distribution that may reduce the risk for cardiovascular disease.

Conflict of Interests

The authors hereby declare that they have no conflict of interests in the submitted paper.

Acknowledgments

The authors acknowledge the Grant from the Indian Council of Medical Research for the purpose of this study. The authors also acknowledge Dr. Satheesh Rao, Professor and Head, Department of Psychiatry for lending the data acquisition system for ECG recording.

References

N. H. Farag, W. A. Bardwell, R. A. Nelesen, J. E. Dimsdale, and P. J. Mills, “Autonomic responses to psychological stress: the influence of menopausal status,” Annals of Behavioral Medicine, vol. 26, no. 2, pp. 134–138, 2003.

G. M. C. Rosano, C. Vitale, G. Marazzi, and M. Volterrani, “Menopause and cardiovascular disease: the evidence,” Climacteric, vol. 10, supplement 1, pp. 19–24, 2007.

F. Ribeiro, G. D. Azevedo, J. C. Crescêncio, et al., “Heart rate variability under resting conditions in postmenopausal and young women,” Brazilian Journal Of Medical and Biological Research, vol. 34, no. 7, pp. 871–877, 2009.

B. M. Curtis and J. H. O'Keefe Jr., “Autonomic tone as a cardiovascular risk factor: the dangers of chronic fight or flight,” Mayo Clinic Proceedings, vol. 77, no. 1, pp. 45–54, 2002.

H. A. Taylor, G. D. Hughes, and R. J. Garrison, “Cardiovascular disease among women residing in rural America: epidemiology, explanations, and challenges,” American Journal of Public Health, vol. 92, no. 4, pp. 548–551, 2002.

C. E. Salmond, J. G. Joseph, and I. A. M. Prior, “Longitudinal analysis of the relationship between blood pressure and migration: the Tokelau island migrant study,” American Journal of Epidemiology, vol. 122, no. 2, pp. 291–301, 1985.

R. Gupta, “Recent trends in coronary heart disease epidemiology in India,” Indian Heart Journal, vol. 60, supplement 2, pp. B4–B18, 2008.

G. Karthikeyan, K. K. Teo, S. Islam et al., “Lipid profile, plasma apolipoproteins, and risk of a first myocardial infarction among Asians. An analysis from the INTERHEART study,” Journal of the American College of Cardiology, vol. 53, no. 3, pp. 244–253, 2009.

G. Broda, C. E. Davis, A. Pajak et al., “Poland and United States collaborative study on cardiovascular epidemiology: a comparison of HDL cholesterol and its subfractions in populations covered by the United States atherosclerosis risk in communities study and the Pol-MONICA project,” Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 16, no. 2, pp. 339–349, 1996.

United Nations, Department of Economic and Social Affairs, and Population Division, “World population prospects: the 2006 revision, highlights,” Working Paper ESA/P/WP. 202, United Nations, New York, NY, 2007.

R. Bannister, Autonomic Failure: A Textbook of Clinical Disorder of Autonomic Neuropathy, Oxford University press, Oxford, UK, 1999.

K. P. Davy, W. L. Willis, and D. R. Seals, “Influence of exercise training on heart rate variability in post-menopausal women with elevated arterial blood pressure,” Clinical Physiology, vol. 17, no. 1, pp. 31–40, 1997.

S. L. Chadha, N. Gopinath, and S. Shekhawat, “Urban-rural differences in the prevalence of coronary heart disease and its risk factors in Delhi,” Bulletin of the World Health Organization, vol. 75, no. 1, pp. 31–38, 1997.

S. P. Singh and P. Sen, “Coronary heart disease: the changing scenario,” Indian Journal of Preventive and Social Medicine, vol. 34, no. 1-2, 2003.

J. Pradeep, M. Z. Idris, R. K. Saran, and S. M. Natu, “A study of coronary heart disease and the associated risk factors in Lucknow district, India,” International Journal of Biological and Medical Research, vol. 4, no. 1, pp. 2966–2972, 2013.

S. Giriappa, Bidi Rolling in Rural Development, chapter 6, Daya Publishing House, New delhi, India, 1987.

D. Gu, X. Wu, K. Reynolds et al., “Cigarette smoking and exposure to environmental tobacco smoke in China: the international collaborative study of cardiovascular disease in asia,” American Journal of Public Health, vol. 94, no. 11, pp. 1972–1976, 2004.

A. R. Hagey and M. P. Warren, “Role of exercise and nutrition in menopause,” Clinical Obstetrics and Gynecology, vol. 51, no. 3, pp. 627–641, 2008.