Journal of Analytical Methods in Chemistry

Volume 2013 (2013), Article ID 353814, 10 pages

http://dx.doi.org/10.1155/2013/353814

Research Article

Validation and Uncertainty Estimation of an Ecofriendly and Stability-Indicating HPLC Method for Determination of Diltiazem in Pharmaceutical Preparations

Fahimeh Sadeghi,¹ Latifeh Navidpour,² Sima Bayat,¹ and Minoo Afshar¹

¹Department of Pharmaceutics, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS), Tehran 193956466, Iran

²Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran 14176, Iran

Received 23 May 2013; Accepted 12 August 2013

Academic Editor: Jesus Simal-Gandara

Copyright © 2013 Fahimeh Sadeghi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract.

A green, simple, and stability-indicating RP-HPLC method was developed for the determination of diltiazem in topical preparations. The separation was based on a C₁₈ analytical column using a mobile phase consisted of ethanol: phosphoric acid solution (pH = 2.5) (35 : 65, v/v). Column temperature was set at 50°C and quantitation was achieved with UV detection at 240 nm. In forced degradation studies, the drug was subjected to oxidation, hydrolysis, photolysis, and heat. The method was validated for specificity, selectivity, linearity, precision, accuracy, and robustness. The applied procedure was found to be linear in diltiazem concentration range of 0.5–50 μg/mL (). Precision was evaluated by replicate analysis in which % relative standard deviation (RSD) values for areas were found below 2.0. The recoveries obtained (99.25%–101.66%) ensured the accuracy of the developed method. The degradation products as well as the pharmaceutical excipients were well resolved from the pure drug. The expanded uncertainty (5.63%) of the method was also estimated from method validation data. Accordingly, the proposed validated and sustainable procedure was proved to be suitable for routine analyzing and stability studies of diltiazem in pharmaceutical preparations.

1. Introduction

Anal fissure is one of the most common and painful proctologic diseases that is effectively treated and prevented with conservative measures in its acute form, whereas chronic fissures may require medical or surgical therapy. Because of the disability associated with surgery for anal fissure and the risk of incontinence, medical alternatives for surgery have been sought. Most recently, pharmacologic approaches that relax the anal smooth muscle, to accomplish reversibly what occurs in surgery, have been used to obtain fissure healing [1–3]. Glyceryl trinitrate ointment (0.2%) has an efficacy of up to 68% in healing chronic anal fissure, but it is associated with headache as the major and most common side effect. Diltiazem, a calcium channel antagonist used in the treatment of angina, hypertension, and arrhythmias, achieved healing of chronic anal fissure comparable to 0.2% Glyceryl trinitrate ointment but caused fewer side effects [4]. Therefore, it is preferred to use diltiazem to treat this chronic disease [5]. The structures of diltiazem and its main impurity are presented in Figure 1.

(a)

(b)

Figure 1: Molecular structures of diltiazem (a) and desacetyl diltiazem (b).

Concerning new therapeutic application of diltiazem, validated and stability-indicating methods should be available to determine this drug in topical preparations. The United States and British Pharmacopeias present two reversed-phase HPLC methods using d-10-comphorsulfonic acid and N,N-dimethyloctylamine, respectively, as amine modifiers suitable for the quantitative analysis of diltiazem in raw material and tablet formulation. However, the use of such ionic additives has fallen somewhat out of favor, as these can have some undesirable effects, including the difficulty of their removal from the stationary phase after use, leading to permanent alteration of its properties and even chemical reaction with some solute types [6]. Moreover, no method was compiled in these pharmacopoeias for the analysis of this medicine in topical preparations. In addition, literature survey reveals little information about the quantitative determination of diltiazem in topical formulations and there is only one report dealing with the analysis of diltiazem in a transdermal gel which has not been validated according to International Conference on Harmonization (ICH) stability-indicating guidelines [7].

Furthermore, in all of the previously reported HPLC methods for determination of diltiazem in pharmaceutical or biological matrixes with UV detection, acetonitrile or methanol has been used as a part of mobile phase or extraction procedure [7–13]. It is worth mentioning that these solvents are ranked by US Environmental Protection Agency (EPA) as hazardous solvents [14] and because of their inherent toxicity [15], safe detoxification of the waste solvents is essential, which may lead to high to very high disposal costs. In recent years, green analytical chemistry has recognized momentum not only in the academic world but also in industrial and pharmaceutical laboratories. The literature of green chemistry has undergone a drastic increase. Green analytical techniques aim to minimize or eliminate the hazardous waste associated with analytical methods [16–20]. Moreover, from an economic point of view, it is reasonable to replace acetonitrile by other solvents (preferably ecofriendly ones) because of its worldwide shortage [21–24].

Testing laboratorie, who wish to comply with the requirements of ISO/IEC 17025:2005 need to estimate uncertainty of measurement for their quantitative methods. Estimation of uncertainty leads to better measurement reliability, renders data from interlaboratory studies comparable, and helps to assess the statistical significance of the difference between the measurement and a relevant reference value [25, 26].

Although few methods for the determination of diltiazem in pharmaceutical matrixes have been reported, information on such metrological parameters is scarce [7–9, 11].

Taking ICH guidelines into consideration [27], the present study describes a simple, validated, and stability-indicating analytical method for the determination of diltiazem in pharmaceutical preparations, which meets the green aspects in analytical chemistry. Also, the calculation of the measurement uncertainty which is based on the validation of the analytical procedures in a laboratory is presented. Moreover, the performance of the method was evaluated and its potential for the determination of diltiazem in pharmaceutical preparations was investigated.

2. Experimental Section

2.1. Materials, Reagents, and Chemicals

Qualified diltiazem hydrochloride standard (99.50%) and pharmaceutical grade diltiazem hydrochloride were kindly provided by Arya pharmaceuticals (Tehran, Iran). All solvents and reagents were of gradient and analytical grade, respectively, and were purchased from Merck (Darmstadt, Germany). Desacetyl diltiazem was synthesized according to the previously reported method [28]. Identification test of desacetyl diltiazem was performed with IR and NMR spectrometry (data not shown). HPLC-grade water was obtained through a Milli-Q system (Millipore, Milford, MA, USA) and was used to prepare all solutions. The pharmaceutical formulations (gel 2%) and the corresponding placebos (mixture of all the excipients) were prepared in our laboratory. Diltiazem organogel 2% manufactured by Troikaa Pharmaceuticals Ltd. (India) was purchased from a local pharmacy.

2.1.1. Preparation of Standard Solutions

Stock standard solution of diltiazem was prepared in methanol at a concentration of 1 mg/mL. This solution was found to be stable for at least 1 month, when it was stored at 2–8°C. Freshly prepared working standards at concentration levels of 0.5, 1, 5, 10, 20, and 50 μg/mL were obtained from stock solution by the appropriate dilution in HPLC-grade water.

2.1.2. Preparation of Test Solutions

A 1.0–1.5 g portion of gel (equivalent to 20–30 mg of diltiazem) was transferred into a 25 mL volumetric flask. Then, 15 mL of HPLC-grade water was added to this portion and the solution was sonicated for 10 min. Thereafter the volume was adjusted to the mark with the same medium to provide a theoretical concentration of 800–1200 μg/mL of diltiazem. This solution was again diluted with HPLC-grade water to make final concentration of 8–12 μg/mL. The experiment was performed in triplicate. These samples were assayed using calibration curves of working standard solutions. The same procedure was applied to placebo to be sure about the selectivity of the method.

2.2. HPLC Analysis

The HPLC method was carried out on a Shimadzu HPLC system (Shimadzu, Kyoto, Japan), set to recycle the mobile phase and was equipped with an SCL-10AVP system controller, LC-10 ADVP pump, DGU-14A degasser, and a SPD-M10AVP PDA detector. The peak areas were integrated automatically by computer using a Shimadzu Class VP software program. A 20 μL volume of sample was introduced into a Rheodyne model 7725i injector.

The elution was carried out on a C₁₈ column (250 mm × 4.6 mm, 5 μm particle size) from Hector (Daejeon, South Korea). All analyses were performed at the column temperature of 50 ± 1°C under isocratic conditions with a mobile phase of ethanol : phosphoric acid solution (pH = 2.5) (35 : 65, v/v) and a flow rate of 2.0 mL/min, using PDA detection at 240 nm.

2.3. Forced Degradation Studies

The stability-indicating capability of the method was determined by subjecting diltiazem solutions (standard and pharmaceutical preparations) at the concentration level of 100 μg/mL to accelerated degradation by acidic, basic, heat, oxidative, and photolytic conditions to evaluate the interferences in the quantitation of diltiazem. Sample solutions prepared in 1 M hydrochloric acid and 1 M sodium hydroxide were used for the acidic and basic hydrolysis, respectively. Both solutions were heated at 70°C for 12 h and then neutralized with basic or acidic solutions, as necessary. For evaluating the heat condition, the sample solutions were heated at 70°C for 12 h. For oxidative degradation, sample solutions were exposed to a solution of hydrogen peroxide (3%) and kept at ambient temperature for 4 h, protected from light. Photodegradation was induced by exposing the sample solution to UV-Lamp at a wavelength of 254 nm placed in a wooden cabinet for 4 hours. The experiments were performed in triplicate. The solutions were diluted with HPLC-grade water to final concentration of 10 μg/mL and were injected into chromatograph.

2.4. Method Validation

The developed method was validated as per the requirements of the ICH guidelines [27]. Linearity was evaluated by determining six working standard solutions at a concentration range of 0.5–50 μg/mL. Five sets of such solutions were prepared. Each set was analyzed to plot a calibration curve. Slope, intercept, and coefficient of determination () of the calibration curves were calculated to ascertain linearity of the method. The limit of quantification (LOQ) was defined as the lowest concentrations with the RSDs lower than 5% and accuracies within ±5%, considering at least ten times the response compared to that of the blank. In order to check the robustness, the effect of small but deliberate variations in the chromatographic conditions was evaluated. The conditions studied were flow rate (altered by ±0.2 mL/min), column temperature (altered by ±2°C), and pH of phosphoric acid solution (altered by ±0.2). These chromatographic variations were evaluated for resolution between diltiazem and desacetyl diltiazem, % assay of the drug, theoretical plates, and tailing factors of the peaks. For method repeatability, assay of working standard solutions (0.5, 5, 10, and 50 μg/mL) was repeatedly performed five times on the same day (intraday). For reproducibility, freshly prepared solutions at aforementioned concentration levels were analyzed at different days (interday) and results were statistically evaluated in terms of % RSD. For recovery studies, 0.125 g portions of preassayed diltiazem gel 2% were spiked with extra 0.5, 1, and 2 mL of stock standard solution. These samples were handled as explained in Section 2.1.2 and the final target levels of 12, 14, and 18 μg/mL were prepared. The concentrations were calculated using calibration curves. Accuracy was calculated as the deviation of the mean from nominal concentration. To assess accuracy, freshly prepared placebo of the diltiazem pharmaceutical formulations was spiked with various amounts of diltiazem to obtain the concentration levels of 0.5, 5, 10, and 50 μg/mL. Each solution was injected in triplicate. Selectivity of this method was indicated by the absence of any excipient interference at retention times of the peaks of diltiazem. The absence of interfering peak was evaluated by injecting a blank sample consisting of diluent and placebo. The double check of the lack of interferences of the resulting by-products with the elution of the peaks of diltiazem was done by calculating the factor, meaning the ratio of the UV molar absorption coefficients of diltiazem at the 240 (peak) and 260 (valley) nm, respectively, using

where and are the diltiazem peaks areas obtained at 240 and 260 nm, respectively. The resulting factors were compared with that of the standard [29]. Moreover, the UV spectrum of each diltiazem peak was acquired during the appearance of the peak in the chromatogram.

2.5. Estimation of the Uncertainty of the Measurements

An expanded uncertainty budget was constructed for diltiazem in pharmaceutical preparations by the RP-HPLC-PDA method according to previously reported procedures [26, 30–32].

Four individual sources were taken into account to assess the expanded uncertainty.

2.5.1. Uncertainty of the Measurement Standard

The uncertainty of the measurement standard is calculated by the quadratic addition of two terms: the uncertainty certified by manufacturer () and the uncertainty corresponding to its preparation by dilution or weighting () [30]:

The stock uncertainty () is calculated from a value given by the manufacturer using

where the purity is expressed as %. When there are independent standard preparations at each concentration level, the term could be eliminated. In this case, the contribution of this term is included in the term [26, 30].

2.5.2. Uncertainty Associated to the Calibration Curve

This value is calculated using [31]

where is the concentration calculated from the calibration curve and is the standard deviation of the concentration, obtained from the calibration curve using

where is the slope of the line, is the -intercept of the line, is the number of replicate unknowns, is the number of the standards, is the mean of repeat measurements of for the sample, is the mean of the values for the calibration standards, are the concentrations of the standards, and is the average concentration of the standards.

2.5.3. Uncertainty Associated to Precision

This value is calculated using

where is the standard deviation of the experimental data for precision and is the number of assays [31].

2.5.4. Uncertainty Associated to Accuracy

This parameter is calculated using (7):

where is the relative standard deviation of the recovery and is the number of assays [31].

The value of the expanded uncertainty was calculated according to ISO GUM guidelines using (8):

where is expanded uncertainty, the coverage factor (for confidence interval 95%, ), and is the concentration of the drug [32].

3. Results and Discussion

3.1. Optimization of the Chromatographic Conditions

The HPLC procedure was optimized with a view to develop a green stability-indicating assay method while keeping the system suitability necessities according to the United States Pharmacopeia, which needs the resolution between diltiazem and desacetyl diltiazem and the number of theoretical plates for the diltiazem peak to be greater than 3 and 1200, respectively.

In order to follow the first principle of green chemistry, acetonitrile was replaced by ethanol in the previously reported mobile phase containing acetonitrile : sodium phosphate monobasic monohydrate buffer (pH 2.5, 0.02 M) (33 : 67 v/v), which was used for the analysis of diltiazem in a transdermal gel [7]. Although ethanol has some shared or similar characteristics to methanol and acetonitrile, including complete miscibility with H₂O, availability in the high purity required for HPLC and low chemical reactivity with most sample species as well as with HPLC, instrument and column surfaces, it has some characteristics that are less favorable. One of them is that the viscosities of ethanol : H₂O solutions are higher than those of methanol : H₂O and acetonitrile : H₂O solutions of the same elution strength at room temperature [33]. To cope with this problem the temperature was raised up to 50°C. This change resulted in lower chromatographic pressure, better peak shapes, good separation of diltiazem and desacetyl diltiazem, and suitable theoretical plates. However, the retention times were not satisfactory when the flow rate was 1 mL/min. Therefore, this parameter was increased to 2 mL/min to resolve the aforementioned problem. According to these preliminary results, the detection wavelength of 240 nm, flow rate of 2 mL/min and the mobile phase of phosphoric acid solution (pH = 2.5) : ethanol (65 : 35, v/v), and the column temperature of 50°C were selected. Under the chromatographic conditions of this method, desacetyl diltiazem and diltiazem were separated completely from each other and their peaks appeared at 4.2 and 5.8 min, respectively (Figure 2), the theoretical plates for diltiazem peak was %, the resolution between diltiazem and its impurity was more than 9, and the tailing factor for diltiazem peak was %. The values of theoretical plates obtained in this study are much higher than those reported previously () [9] which indicates better column efficacies at the chromatographic conditions used in this study. Before fully implemented in the quantitative determination of drug substance and pharmaceutical preparation, this method was thoroughly validated according to ICH guidelines [27].

Figure 2: Typical chromatogram of diltiazem and its main impurity (desacetyl diltiazem); peak 2: desacetyl diltiazem, peak 1: diltiazem.

3.2. Forced Degradation Studies

Stability-indicating method is defined as an analytical method that accurately quantifies the active ingredients without interference from degradation products, process impurities, excipients, or other potential impurities [34]. Diltiazem showed drastic degradation in acidic, basic, and photolytic conditions; in that only , , and % of the drug remained, respectively, and at the same time a remarkable increase in the concentrations of desacetyl diltiazem was observed (Figures 3(c), 3(d), and 3(f)). The first-order hydrolysis of diltiazem in the presence of acids and bases and formation of desacetyl diltiazen in these conditions has been reported previously, which confirms the results of this study [35]. Andrisano et al. showed that the main photoproduct of diltiazen after irradiation of UV-A and UV-B is diltiazem-S-oxide [36]. However, according to the findings of the present study the main photoproduct after exposure of an aqueous solution of diltiazem to UV-C is desacetyl diltiazem, indicating a different photodegradation process. Under the oxidative and heat conditions, the diltiazem contents decreased to and %, respectively, with a minor increase in the amounts of desacetyl diltiazem (Figures 3(b) and 3(e)). The degradation products of the parent compound were found to be similar for both the pharmaceutical and standard solutions. All the degradation studies are summarized in Table 1.

Table 1: Summary of stress degradation studies of diltiazem.

Stress condition/media/duration
Recovered diltiazem (%)

mean (RSD, %)
No. of observed impurities

Photolytic/H₂O/254 nm/4 h
48.86 (1.48)
1

Acidic/1.0 N HCl/70°C/12 h
16.67 (1.95)
1

Neutral/H₂O/70°C/12 h
84.57 (0.86)
1

Oxidative/3.0% H₂O₂/4 h
89.01 (1.74)
1

Basic/1.0 N NaOH/70°C/12 h
10.47 (2.10)
2

(a)

(b)

(c)

(d)

(e)

(f)

Figure 3: Typical chromatograms of (a) diltiazem working standard solution (10 μg/mL) and after degradation under (b) heat condition; (c) acidic hydrolysis; (d) basic hydrolysis; (e) oxidative condition: peak 3: hydrogen peroxide, peak 4: oxidative impurity; (f) photolytic condition: peak 1: diltiazem, peak 2: desacetyldiltiazem.

3.3. Method Validation

3.3.1. Selectivity and Specificity

Specificity is the ability of the method to unequivocally assess the analyte response in the presence of its potential impurities that was illustrated by the complete separation of diltiazem from degradation products as shown in Figure 3. Furthermore, the decreases observed in diltiazem contents in stability studies, when degradation products appeared, proved the specificity of the method (Table 1). Also, the factors of the diltiazem in stress solutions (3.52–3.55) show the same values of that of the standard (3.55). The UV spectrum obtained during the appearance of the peaks also confirms their purity (Figure 4). Consequently, the forced degradation studies documented the stability-indicating power and specificity of the proposed method.

(a)

(b)

(c)

(d)

(e)

(f)

Figure 4: Typical spectrums of (a) diltiazem working standard solution and after degradation under (b) heat condition; (c) acidic hydrolysis; (d) basic hydrolysis; (e) oxidative condition; (f) photolytic condition.

The application of the whole procedure to placebo samples in order to verify the method selectivity demonstrates that no interferences were detected (Figure 5(b)).

(a)  

(b)

Figure 5: A chromatogram obtained from analyzing the commercially available gel. The solution contains the target diltiazem concentration of 9.35 μg/mL. (b) blank.

3.3.2. Linearity, Precision, and LOQ

Linearity was determined by constructing five independent calibration curves, each one with six calibration points of diltiazem, including the LOQ, in the range of 0.5–50 μg/mL. The peak areas of diltiazem against the respective concentrations were used for plotting the graph, and the linearity was evaluated by the least square regression analysis. The linearity curve was defined by the following equation: (), which indicated the linearity of the calibration curve for the method. Moreover, the relative standard error of slope can be used as a parameter with respect to the precision of the regression, as a general acceptance criterion for the linearity performance of the analytical procedure [37]. This parameter should be comparable to the calculated RSD in the evaluation of the precision. In this study, the result obtained for the RSD of the slopes was 1.22% which is comparable to mean value 1.36% of the RSD of the precision.

Summary of the method validation results is shown in Table 2. The method was proved to be precise, as the intra- and interday precision calculated for the concentration levels of 5, 10 and 50 μg/mL ranged from 0.78% to 1.46% and from 1.18% to 1.53%, respectively. These values fulfill the validation criteria of an analytical method designed for quality control of pharmaceutical preparations for which RSD values <2% are acceptable [37].

Table 2: Precision, accuracy, and recovery data for the proposed method.

Diltiazem

concentration

(µg/mL)
Precision (RSD, %)
Accuracy ()

mean (RSD, %)
Recovery ()

Intraday 

()
Interday

()
Target

concentration

(µg/mL)
Calculated

concentration µg/mL (mean)
%
±SD

0.50
0.96
2.40
96.67 (0.91)
12.00
11.91
99.25
0.13

5.00
1.34
1.53
100.01 (1.32)
14.00
14.08
100.57
0.07

10.00
0.78
1.18
99.06 (0.67)
18.00
18.30
101.66
0.29

50.00
1.46
1.19
98.78 (0.59)

The LOQ is the lowest concentration that can be quantified with acceptable precision and accuracy. The LOQ of diltiazem was determined to be 0.5 μg/mL, considering the mean accuracy value of 96.67% and RSD value of 0.91% (Table 2). These values indicate that the proposed method is much more sensitive than what have been reported previously for analysis of diltiazem in a transdermal gel [7]. Diltiazem is a basic compound and consequently prone to peak tailing and poor peak efficiency; therefore, sensitivity of the method is of paramount importance to resolve the mentioned problem because one of the factors which can end up to peak asymmetry is sample overload [6].

3.3.3. Recovery and Accuracy

The accuracy was evaluated applying the proposed method to the analysis of the in-house mixture of the gel excipients with known amounts of the drug, to obtain solutions at concentration levels of 0.5, 5, and 10 and 50 μg/mL. The accuracy was assessed from three replicate determinations and calculated as the percentage of the drug recovered from the formulation matrix. The mean and RSD values calculated for the analysis of three diltiazem concentration levels of 5, 10, and 50 μg/mL are shown in Table 2; the mean values were found to be 100.1%, 99.06%, and 98.78% with RSDs 1.32%, 0.67%, and 0.59%, respectively, demonstrating that the method is accurate within the desired range. Also, the results obtained from the analysis of preassayed pharmaceutical preparations, spiked with different amounts of diltiazem stock solution, revealed acceptable recoveries with the mean value of 100.49 and %RSDs < 1.61, respectively. These values document a high recovery in this method.

3.3.4. Robustness

Chromatographic parameters including % of assay, resolution between diltiazem and its impurity, theoretical plates, and tailing factor of diltiazem peaks were not significantly affected by the slight changes in the chromatographic conditions like alteration in flow rates, pH of the aqueous solution of mobile phase, and column temperature. Analysis was carried out in triplicate and only one parameter was changed in the experiments at a time. The results of the experimental variables evaluated were within the acceptable deviation (RSD < 2%), the resolution of the aforementioned peaks was more than 9, and the theoretical plates and tailing factor parameters were calculated to be more than 11500 and less than 1.2, respectively, indicating that the proposed method is robust under the conditions tested.

3.3.5. The Uncertainty of the Method

The expanded uncertainty of the method for quantification of diltiazem in pharmaceutical preparations was calculated. The results are shown in Table 3. Among the four sources of uncertainty, which were taken into consideration, the uncertainty associated with calibration appears to be the most important source in the overall uncertainty. Therefore, analysts should pay great attention when conducting calibrations of instrumental techniques.

Table 3: Partial and expanded uncertainties associated to the analytical results (expressed as % relative standard deviation).

Uncertainties
Diltiazem

0.29

(%)
2.58

(%)
1.01

(%)
0.39

(%)
5.63

(µg/mL)
0.52

Concentration (µg/mL)
9.30

3.3.6. Application of the Method

The optimized and validated method was applied to the determination of diltiazem in marketed gels. The amount of diltiazem in gels was calculated using calibration curve method. Typical chromatogram obtained following the assay of the pharmaceutical dosage form is shown in (Figure 5(a)). The results of the assay undertaken and the calculated uncertainties are shown in Table 3. The value of 99.46% of label claim indicates that the method is selective for the analysis of diltiazem without interference from the excipients used to formulate and produce these gels.

4. Conclusion

The aim of this study was to develop a green and specific method for the determination of diltiazem in pharmaceutical preparations. The method was designed to be specific, selective, sensitive, robust, reproducible, accurate, inexpensive, and easy to perform. The principal advantage of the method is the use of available environmentally friendly solvents and reagents for LC analyzing and extractions to follow the first principle of green chemistry which emphasizes waste prevention instead of remediation [15]. To the best of our knowledge, this is the first method which is thoroughly green and reports the metrological parameters in quantification of diltiazem in pharmaceutical dosage forms. In addition, recycling significantly reduced the mobile phase consumption and made the method economic. Moreover, the method is much more sensitive than the previously reported procedure [7].

Finally, the newly developed method was successfully performed for the analysis of diltiazem in pharmaceutical preparations and it can thus be used for routine analysis, quality control, and studies of the stability of pharmaceutical formulations containing diltiazem.

Conflict of Interests

The authors declared no conflict of interests.

Acknowledgments

The authors extend their appreciation to Arya Pharmaceutical Co. for kind donation of diltiazem standard and raw material. This work was financially supported by Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS).

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