Qualitative and Quantitative Characterization of Monosaccharide Components of Salvia miltiorrhiza, Liguspyragine Hydrochloride, and Glucose Injection

Salvia miltiorrhiza, liguspyragine hydrochloride, and glucose injection (SLGI) was made of Salvia miltiorrhiza Bge., liguspyragine hydrochloride, glucose, and glycerin. There were many kinds of monosaccharide components in SLGI, which might be from the raw material and Salvia miltiorrhiza Bge. Separation was performed on a Phenomenex Luna C18 analytical column (250 mm × 4.6 mm i.d., 5 μm, AccuStandard Inc., USA) at 30°C. The mobile phase consisted of two solvents: 0.1 mol/L phosphate-buffered saline (pH 6.7) (solvent A) and acetonitrile (solvent B) with gradient elution. The flow rate was maintained at 1.0 mL/min. Five kinds of monosaccharide components, glucose, D-mannose, L-rhamnose monohydrate, galactose, and xylose, were detected by precolumn derivatization HPLC, and their contents were compared with each other. And finally, concentrations of glucose in SLGI were determined and they were higher than the values of marked amount, which showed that one source of glucose might be from Salvia miltiorrhiza Bge. in SLGI. The average concentration of glucose was 5.18 g/100 mL, which was near the average value at 5.25 g/100 mL detected by ultraviolet spectrophotometry and also close to the marked amount (5.00 g/100 mL) on the label.


Introduction
In China, a large number of compound preparations are registered as chemical drugs; in spite of that they are from traditional Chinese medicine (TCM) and natural medicine, especially some injection. The levels of quality standard and quality control of compound preparations registered as chemical drugs are higher than Chinese medicine approval. However, the quality control system of TCM is not well built, especially the injections registered as chemical drugs from TCM and natural medicine. Compared to the classic chemical drugs, these injections are of various ingredients. Normally, the injections are from Chinese medicine extract with high purity as an intermediate, drug substance, medical supplements, and so forth. The classic example is Salvia miltiorrhiza, liguspyragine hydrochloride, and glucose injection (SLGI), which is made of Salvia miltiorrhiza Bge., liguspyragine hydrochloride, glucose, and glycerin. SLGI was used for occlusive ischemic cerebrovascular disease and other vascular diseases for that it can promote antiplatelet aggregation, expand coronary artery, reduce blood viscosity, speed up the flow rate of the red blood cells, and improve microcirculation against myocardial ischemia and myocardial infarction [1]. The quality control is carried out via quantification of one or several components from TCM.
However, only danshensu and liguspyragine hydrochloride are the quality indexes according to the quality standard, not considering the other components, such as glucose and salvianolic acid compositions. There were glucose, Lrhamnose monohydrate, xylose, D-mannose, galactose, and other carbohydrate compositions in Salvia miltiorrhiza Bge. So, sources of glucose in SLGI were from the raw material 2 Journal of Analytical Methods in Chemistry and Salvia miltiorrhiza Bge. through the study of production processes. And the above monosaccharides may be in SLGI [2].
Most of the literatures on the pharmacological action and clinical application of SLGI were studied. So far no studies reported on the determination of monosaccharide content. The common method used to determine the monosaccharide components is UV-Vis spectrophotometric method, which also determines the total amount of all monosaccharide components without selectivity. And in this research the qualitative identification and quantification of monosaccharide components are implemented by precolumn derivatization HPLC [3][4][5][6][7].

Sample Solutions for HPLC-UV.
The SLGI solutions were stored at 4 ∘ C. The solutions were brought to room temperature, diluted, and filtered through a 0.45 m membrane filter before analysis.
The samples of SLGI for HPLC-UV analysis were injected for HPLC-UV analysis. [8,9]. PMP, 3-methyl-1-phenyl-2-pyrazolin-5-one, and monosaccharides produce monosaccharides-PMP monosaccharide derivatives under the condition of alkaline and quantitative condensation. 21.775 g of PMP was weighed, dissolved in 250 mL with methanol, and then filtered through a 0.45 m membrane filter before analysis.

Standard Solutions.
Each reference was accurately weighed, dissolved in deionized water, and diluted to the appropriate concentration. And the standard solutions were filtered through a 0.45 m membrane filter before analysis.

Derivatization Method.
The SLGI and standard solutions derivatization process was dealt with with the following the procedure. The SLGI sample was accurately drawn and mixed with 200 L of the sodium hydroxide solution (0.3 mol/L) and 160 L of PMP solution (0.5 mol/L), separately. Then the mixed solution was carried out upon a water bath and heated for 30 min and cooled down to room temperature. Next, 200 L of the hydrochloric acid solution (0.3 mol/L) was injected to neutralize the mixed solution. And then, 1,240 L of purified water and 2 mL of chloroform were added in the prior mixed solution and well mixed, which was centrifuged at 3,000 rpm for 5 minutes, discarding the chloroform layer to collect the water layer. After extraction for 3 times, the water layers were collected and filtered through a 0.45 m membrane filter before analysis.

HPLC Apparatus and
Conditions. HPLC-UV analysis was performed using an UltiMate 3000 HPLC-UV system (Thermo Fisher Scientific, New York, USA) compressing a vacuum degasser, binary pump (SR-3000), autosampler (WPS-3000), thermostatted column compartment (TCC-3000), and a diode array detector (DAD-3000); scanning from 200 to 400 nm, the wavelength was then selected and fixed at 250 nm for qualitative and quantitative analysis [10], considering the variety of constituents in SLGI. Separation was performed on a Phenomenex Luna C 18 analytical column (250 mm × 4.6 mm i.d., 5 m, AccuStandard Inc., USA) at 30 ∘ C. The mobile phase consisted of two solvents: 0.1 mol/L phosphate-buffered saline (pH 6.7) (solvent A) and acetonitrile (solvent B) with gradient elution (0-3 min, 13-13% B; 3-8 min, 13-16% B; 8-15 min, 16-23% B; 15-30 min, 23-23% B). Reequilibration duration was ten min between individual runs. The flow rate was maintained at 1.0 mL/min and 10 L of sample solution was injected in each run. [11]. Column types, mobile phase compositions, gradient elution procedure, flow rate of the mobile phase, and column temperature were optimized, respectively, to achieve good separation of as many peaks as possible within a short analysis time. Different mobile phase systems, such as acetonitrile/water, methanol/water, acetonitrile/0.1 mol/L ammonium acetate, and acetonitrile/0.1 mol/L phosphate-buffered saline (pH 6.7), were tested. Results showed that addition of acetonitrile/0.1 mol/L phosphate-buffered saline (pH 6.7) was more suitable to obtain better peak symmetry and stable baseline and/or to inhibit ionization of the acidic ingredients in SLGI. The applicability of the established analysis method to different HPLC instrument systems has been verified. The detection wavelength was selected with the use of a DAD detector. The detection wavelength for quantitative analysis was selected according to the maximum adsorption wavelengths of D-mannose, L-rhamnose monohydrate, glucose, galactose, and xylose at 250 nm, as shown in Figure 1. The typical chromatogram of SLGI was illustrated in Figure 2, in which there were D-mannose, L-rhamnose monohydrate, glucose, and xylose in SLGI.

Method Validation of Quantitative Analysis.
The HPLC method was validated defining the linearity, limits of quantification, and detection, identification and quantification of the Table 1: Precision, repeatability, stability, and recovery of glucose in in SLGI ( = 6).  analyte, repeatability, precision, stability, and recovery. The work was performed under the validation guide according to the "0512 high performance liquid chromatography" in Chinese Pharmacopoeia Part IV (2015 edition).

Precision, Repeatability, Stability, and
Recovery. Intraday variation was chosen to determine the precision of the developed assay. The SLGI sample solutions were analyzed for six replicates within one day. Variation was expressed by the RSD, which was less than 1.0% (Table 1). Six different sample solutions prepared from the same sample were analyzed to confirm the repeatability of the developed assay. RSD values As (mAU * min) As (mAU * min)  of the compound's contents were all less than 2.0%, which satisfied the criteria of quantitative analysis (Table 1). For the stability test, peak areas of glucose in sample solution, which was stored at room temperature in the dark, were analyzed at 0 h, 4 h, 8 h, 16 h, and 24 h. RSD value of the content was less than 1.0% (Table 1). These results expressed the view that it was feasible to analyze the samples within one day. The ratio between amounts determined and spiked was considered to be able to display the accuracy of the method. Known amounts (low, medium, and high) of the glucose reference were spiked into samples and then prepared as test solutions.
The determination was performed in triplicate, and the average recoveries and RSD were calculated. The developed method had good accuracy with the overall recovery at 102.9%, with the RSD ranging at 3.36% (Table 1). These results indicate that the HPLC-UV method is precise, accurate, and sensitive for the quantitative determination of glucose in SLGI samples.

Chemical Profiling and Quantitation.
Chemical profiling and assay of 10 different commercial batches of SLGI were performed. As for the quantitative analysis, glucose was invoked as the marker component in the enacted quality standard of SLGI recorded in the national standard for Chinese patent medicine. The developed determination method was subsequently applied to determination of glucose in 10 different commercial batches of SLGI. The results are presented in Table 2. The content of glucose varied bittily, and the RSD (%) of contents was 2.20. There was no obvious difference about the concentrations of glucose in 10 batches of SLGI. And the average concentration of glucose was 5.18 g/100 mL, which was near the average value at 5.25 g/100 mL detected by ultraviolet spectrophotometry and also close to the marked amount (5.00 g/100 mL) on the label. Figures 1 and 2, there were D-mannose, L-rhamnose, glucose, and xylose in SLGI. But the peak areas of D-mannose, L-rhamnose monohydrate, and xylose were much smaller than the peak area of glucose (Table 3). That is to say, the main monosaccharide was glucose in SLGI. And the ratio of the peak area of glucose to the total peak area of monosaccharide (G/T) was higher than 98%. So the determination of glucose could characterize the total content of monosaccharide in SLGI.

Conclusion
Precolumn derivatization HPLC method, as shown in the paper, could clearly show the content of each component rather than the total amount of all monosaccharide components without selectivity, which could be more intuitive for evaluation of the quality of the SLGI attributes. And many studies on monosaccharide components, amino acids, salvianolic acids, and other substances are carried out to reveal the material basis of SLGI. In current quality standard of SLGI, however, there is no mandatory requirement of the above components according to The Standard Issued by the Ministry of Health (number WS-10001-(HD-1136)-2002). As a valuable supplement, the method may provide a new way to improve the quality control of compound preparations, such as TCM compound preparations.

Conflicts of Interest
The authors have declared no conflicts of interest.