Analysis of the HPLC Fingerprint and QAMS for Sanhuang Gypsum Soup

A valid and encyclopaedic evaluation method for assessing the quality of Sanhuang Gypsum Soup (SGS) has been set up based on analysis of high-performance liquid chromatography (HPLC) fingerprint combined with the quantitative analysis of multicomponents by single marker (QAMS) method, hierarchical cluster analysis (HCA), and similarity analysis. 20 peaks of the common model were obtained and used for the similarity analysis and HCA analysis. Berberine was selected as an internal reference, and the relative correction factors of mangiferin, geniposide, liquiritin, epiberberine, coptisine, baicalin, palmatine, harpagosid, wogonoside, cinnamic acid, cinnamic aldehyde, baicalein, glycyrrhizic acid, and wogonin were established. The accuracy of quantitative analysis of multicomponents by the single-marker method was verified by comparing the contents of the fourteen components calculated by the external standard method with those of the quantitative analysis of multicomponents by the single-marker method. No significant difference was found in the quantitative results of the established quantitative analysis of multicomponents by a single-marker method and an external standard method. In summary, these methods were applied to evaluate the quality of SGS successfully. As a result, these evaluation methods have great potential to be widely used in the quality control of traditional Chinese medicines (TCM).


Introduction
Perspiration is a considerable physiological phenomenon to maintain and control body temperature. Excessive sweat secretion can cause armpit moisture, resulting in unpleasant body odour, embarrassment, and inconvenience [1,2]. Hyperhidrosis is an excessive sweating disease which can bring severe psychological burden and affect the quality of life of patients negatively.
ere are a variety of medical treatments and surgery for treating primary hyperhidrosis. However, the side effects of these drugs include thirst, dry eyes, dizziness, drowsiness, constipation, and urinary retention, which limit the scope of their use. Surgical treatment is mainly applied to patients who are not suitable for the abovementioned methods. Surgery is more traumatized and risky than other treatments. erefore, surgical operation should be as a second or third option [3].
ere is still a paucity of effective nonsurgical therapies. With the development of modern society, the elimination of body odour is given more and more people's attention; this article is about introducing Sanhuang gypsum soup (SGS) which is a significant antiperspirant. rough the use of classical Chinese medicine SGS to regulate the internal environment of the human body, it achieves a good antiperspirant effect with small side and remarkable effects, which make up for deficiency of some medicine and surgery. Every year, more than ten thousands of patients have benefited from SGS by reducing excessive perspiration symptoms. SGS is a hospital preparation of Jiangsu Provincial Hospital of Traditional Chinese Medicine, which consists of coptidis rhizoma, phellodendri chinensis cortex, scutellariae radix, scrophulariae radix, anemarrhenae rhizome, gardeniae fructus, cinnamomi cortex, glycyrrhizae radix et rhizoma preparata cum melle, and gypsum fibrosum. ese herbs can be used for treating and could exhibit action on excessive perspiration through anti-inflammatory and antipyretic properties. Studies have shown that SGS has many chemical constituents such as mangiferin, geniposide, coptisine, wogonin, wogonoside, baicalein, baicalinin, and cinnamic aldehyde [4][5][6][7][8][9][10][11][12][13] in accordance with herbs and preparations known to be beneficial for the treatment of excessive perspiration through anti-inflammatory properties and so on.
Although the SGS is prepared as a prescription with the combination of these herbs in well-defined formulae, no standard quality control method for this product has been reported up to now. Since the effect of SGS might result from the synergy of multiple components, a reliable, sensitive, and uncomplicated quantitative method based on the diverse constituents is need to be developed.
Our findings have established an HPLC method to evaluate the quality of SGS comprehensively. Due to the variety of components of traditional Chinese medicine preparations, any one of the active ingredients cannot reflect the overall curative effect of traditional Chinese medicine. erefore, a comprehensive macroscopic analysis will become an inevitable trend. Chromatographic fingerprint analysis with integrated, macroscopic, and "fuzzy" nonlinear characteristics is more adapted to the traditional Chinese medicine theory needs. Under the premise of efficacy, toxicology, and clinical trials which have confirmed safety and efficacy of preparation, we can not only verify the authenticity of the preparation but also determine whether the stability of the quality exists or not along with a practical fingerprint. Unlike the content determination, the fingerprint can provide more informative and useful message than the determination of any single component. e US Food and Drug Administration (FDA) allows applicants to provide product chromatographic fingerprinting information in the phytomedical guidance (Draft for Comment). British Herbal Codex, Ayurvedic Codex, the Canadian Society of Medicinal and Aromatic Plants [14], and the German Society of Medicinal Plants [15] also accept chromatographic fingerprint. One of the first measures that China's State Drug Administration has taken to strengthen the supervision of traditional Chinese medicine injections requires the research on the fingerprint of injections, which has taken into account its necessity and feasibility. It is accepted that preparation of acceptable quality can be exerted on its drug efficacy, what really matters is establishing an accurate and easy method.
Previously, our laboratory has researched on the fingerprints of the existing preparations which have been applied in the control of preparation quality. Also, to make up for the limitations of fingerprint that cannot be quantified accurately, a QAMS method using berberine as the standard was developed and validated for the simultaneous quantitative of 14 components [16].
is strategy can not only reduce the cost of the experiment and time of detection but also be independent of the availability of all the target ingredients [15]. To our knowledge, quality control of herb extracts and botanical ingredient by QAMS have been included both in USP 33-NF and in Ch.P.2010 edition (volume I).
Our results showed that no significant difference was found in the results between our established QAMS method and the external standard method. No one has yet studied the fingerprints of SGS; this article first established the SGS fingerprinting method and also used the QAMS method to measure the preparation of 14 kinds of pharmacodynamics components. is method could potentially be applied for the identification of qualitative and quantitative quality of SGS.
is HPLC fingerprint method, therefore, provides a comprehensive platform for quality evaluation of SGS with more chemical information. e combination of HCA and similarity analysis presents the differences and similarities of the HPLC fingerprints. In the meantime, QAMS method was adopted to quantify the main active components by comparing with the external standard method (ESM) in all the SGS samples. Our findings offer a new routine for assessing the quality of TCM.
BP-211D electronic analytical balance (Germany Sartorius Company) was used to weigh the drugs. Sonicator (SK6200H, Shanghai Branch guided ultrasound instrument Co., Ltd.) was used to help dissolve the sample.

Materials.
e batch numbers and origins of eight qualified Chinese herbal pieces of decoction are shown in Table 1. All pieces were purchased from Anhui Concord Pharmaceutical Pieces Co., Ltd. and identified by Professor Zhihui Liu of Nanjing University of Traditional Chinese Medicine.
Fifteen batches of Sanhuang Gypsum Soup were provided by the Department of Pharmacy of Jiangsu Provincial Hospital.
A Milli-Q water (Millipore, Inc., USA) purification system was applied to purify water for the HPLC analysis.

Preparation of the Sample Solution.
e sample solution of SGS was precisely absorbed (5 ml) and immersed in 25 mL volumetric flask with methanol. Additional methanol was added to compensate the weight loss after ultrasonic extraction for 30 min. All solutions were filtered through 0.45 μm filter membranes before being injected into the HPLC system precisely.

Data Analysis.
e data were analyzed and evaluated by Similarity Evaluation System for chromatographic fingerprint of TCM (Version 2004 A) which was recommended by the SFDA of China for evaluating similarities of chromatographic profiles of TCM. e similarity among different chromatograms was determined by calculating the correlative coefficient or cosine value of the vectorial angle [17][18][19]. HCA was carried out by calculating Squared Euclidean distance to distinguish preparation of different batches using SPSS. At the same time, we used the external standard method (ESM) and QAMS to calculate the 15 active components in 13 batches of SGS, respectively, to verify the feasibility of QAMS.

Chromatograph Optimization.
At present, there are no single liquid phase conditions that can divide 15 components of SGS with good resolution. As the ingredients of SGS are very intricate, it is critical to establish a favorable mobile phase system, gradient elution system, and detection wavelength to obtain efficient separation of the numerous target components. e suitable ingredient of the HPLC method was investigated by checking peak resolution and the peak purity of SGS. In this case, some different mobile phases were tested which were acetonitrile-water, methanolwater, methanol-water containing phosphoric acid or formic acid at different concentrations, acetonitrile-water with acetic acid, formic acid, and phosphoric acid at different concentrations. Experimental results show that acetonitrilewater containing 0.2% phosphoric acid system produced       Journal of Analytical Methods in Chemistry  Figure 1: (a) Mixed standard solution, (b) negative sample without coptidis rhizoma, (c) negative sample without scrophulariae radix, (d) negative sample without anemarrhenae rhizome, (e) negative sample without gardeniae fructus, (f ) negative sample without coptidis rhizoma and phellodendri chinensis cortex, (g) negative sample without glycyrrhizae radix et rhizoma preparata cum melle, (h) negative sample without cinnamomi cortex, (i) negative sample without phellodendri chinensis cortex, (j) negative sample without scutellariae radix, and (k) SGS sample. 1, mangiferin; 2, geniposide; 3, liquiritin; 4, epiberberine; 5, coptisine; 6, baicalin; 7, palmatine; 8, berberine; 9, harpagosid; 10, wogonoside; 10, cinnamic acid; 11, cinnamic aldehyde; 12, baicalein; 13, glycyrrhizic acid; and 14, wogonin.  S9 S10 S11 S12 S13 R Figure 3: Fingerprint chromatograms of 13 batches of SGS. Journal of Analytical Methods in Chemistry sharp and symmetrical chromatographic peak shapes, good separation, and prevented the peak tailing. Chromatogram with the maximum number of peaks also relies on best conditions for preparation of sample solution. On the basis of the investigation of different solvent and ultrasonic time, it can be concluded that samples are dissolved in methanol and ultrasound 30 minutes; we can get better resolution and reproducibility of fingerprint chromatograms under the conditions of Section 2.6. Under the above chromatographic conditions, all the components were well separated (Figure 1).

Linearity.
A mixed solution containing all the reference substances were prepared and diluted in series with methanol to obtain six different concentrations. e different concentration of the mixed solution was used for constructing the reference curve. As shown in Table 2, good calibration curves of 15 compounds were obtained, and high correlation coefficient values (R 2 > 0.999) were shown with good linearity at a wide range relatively. In response to sample concentration, the peak area of the analyte is determined by least squares linear regression to obtain a linear equation.     e stability of the sample solutions was analyzed at 0, 2, 4, 8, 12, and 24 h at room temperature. It was found that the sample solutions were stable within 24 h (RSD ≤ 5.0%).

Journal of Analytical Methods in Chemistry
To confirm the repeatability of the method, six independently prepared solutions from the same batch were analyzed. e RSD values of the peak area was 0.37%, 0.31%, 1.52%, 0.72%, 1.00%, 0.20%, 0.71%, 0.18%, 0.88%, 0.50%, 3.02%, 3.65%, 2.30%, 1.68%, and 3.23%, respectively. e results indicated the method is reproducible. e recovery was performed by adding a known amount of individual standards into a certain amount of the SGS sample. e mixture was extracted and analyzed by using the method mentioned above. e average recoveries of 6 samples are shown in Table 3.
e results show that the method is accurate. e recoveries of the 15 compounds which are shown in Table 3 ranged from 70.08% to 111.45% with RSDs ≤ 5.0%.

HPLC Fingerprint and Similarity Analysis.
irteen batches of samples were prepared according to Section 2.5, and 10 μL was injected into the HPLC system according to the chromatographic conditions given under Section 2.6, and then the chromatograms were recorded and entered into the similarity analysis software. We selected S (1) as the reference chromatogram, the utilization of the average correlation coefficient method of 13 batches of samples for multipoint correction, time window width is set to 0.5, while the establishment of a common model is to generate a control fingerprinting SGS, the antithesis fingerprint chromatogram was shown in Figure 2. Fingerprint chromatograms of 13 batches of SGS can be seen in Figure 3. As compared with the reference fingerprint chromatograms, the similarities of 13 batches of samples shown in Table 4, and the results are all above 0.95. On the basis of these results, we concluded that SGS between different batches are of good consistency and in line with the relevant requirements of the fingerprints. Palmatine is the main active ingredient of coptidis rhizoma; the corresponding peaks have favorable resolution, and the retention time is stable and moderate. erefore, we selected palmatine (no. 11 peak) as the reference peak and calculated the relative retention time of the other common peaks. We can see that the retention time of the common peak is stable. According to the retention time of each fingerprint, a total of 20 common peaks were identified while 14 of them were determined. However, it should be pointed out that the chemical property of cinnamic aldehyde is very unstable due to its alkene structure of the molecule which has poor stability when exposed to light and oxygen, so it is not within the category of the common peaks [20].

Hierarchical Cluster Analysis.
e 13 * 20 matrices were obtained from 20 common peak areas of fingerprints of 13 batches of SGS. e cluster analysis was performed by using spss 2.0 software. e Euclidean distance was chosen as the measure of the distance between groups. e results are shown in Figure 5. S3, S4, S5, and S12 batches of samples are divided into a category; the remaining batches are divided into another classification, which indicates that there are differences in the content of the components in the samples prepared from different raw material TCM pieces. And it suggested that HCA was a valid method for the identification of the source of TCM pieces.

Quantitative Analysis of Multicomponents by a Single Marker (QAMS).
It is well established that many variations of experimental conditions, such as concentrations of standard, detector, and peak measurement parameters, would extremely influence the RCFs. Accordingly, the accuracy of RCFs may affect the final analysis results. Nevertheless, RCFs, which was calculated by linear-regression equation in the experiment, was considered to be accurate and stable [21,22]. e RCFs were calculated using the calibration curves as follows: e content of the measured component was calculated as follows: a s is the ratio of the slope of internal standard reference calibration equations; a k is the ratio of the slope of measured component calibration equations; A K is the peak area of the measured component; A S is the peak area of the internal standard reference. We investigated the influence of different instruments and different columns on the RCF values, and results are shown in Table 5 which illustrated RCF values had good repeatability on different chromatographic systems and different columns.
In this paper, we selected cheap, readily available, and chemically stable berberine as an internal reference standard for the quantitative determination of other active components. In addition to that, berberine is the main active ingredient of phellodendri chinensis cortex and coptidis rhizoma, so this study eventually takes it as an internal reference standard. e relative retention time has been used to locate target chromatographic peaks.
where t s is the retention time of internal standard reference and t k is the retention time of measured component. e internal reference is berberine; the relative retentions between the other target peaks and berberine were obtained in different columns and HPLC instruments. Results are shown in Table 6. e results showed that their RSDs ≤ 5% and no interference with other components; the relative retention time can be applied to locate the peak component of the analytes.
We measured the multicomponent content of 13 batches SGS ( Figure 6); the results showed that there were significant differences in some contents of 15 ingredients, such as cinnamic aldehyde and baicalein, which indicated that only a few ingredients of the standard determination of content could not control the quality of SGS effectively. It is necessary to use multiple active ingredients as index components to control the quality of TCM preparations more comprehensively.
To validate the difference between ESM and QAMS method using RCFs, 13 SGS samples were analyzed for their active ingredients. e calculated results are shown in Table 7. Standard method difference (SMD) is calculated according to the following equation: where C ES and C QAMS represent the concentrations of an analyte assayed by the external standard method and QAMS method, respectively [23]. All the values of standard deviation (SMD < 0.05) revealed that there were no significant differences between ESM and QAMS methods of all SGS samples.

Conclusion
On the basis of these results, we concluded that HPLC fingerprint method based on chemical constituents profiling was an effective and stable tool, and QAMS method was feasible to quantify the active compounds by RCFs for evaluating the quality of SGS. Along with similarity analysis and HCA of synthesis, the quality of SGS would be evaluated and better identified comprehensively. is method could potentially be applied in the quality control of TCM.
Data Availability e datasets used or analyzed during the current study are available from the corresponding author on reasonable request. All data generated or analyzed during this study are included in this submitted manuscript.

Disclosure
Minghui Dong is the co-first author.

Conflicts of Interest
e authors declare no conflicts of interest.