Phenolic Acid Content and Antioxidant Properties of Extruded Corn Snacks Enriched with Kale

Prohealth food contains specific components which have positive influence on the health and well-being of the consumer. An important position among bioactive compounds occurs for polyphenols. Many results have indicated that an increased intake of phenolic compounds may reduce the risk of cardiovascular diseases and type 2 diabetes. The objective of the study was production of extruded corn snacks with addition (0, 2, 4, 6, and 8%) of kale (Brassica oleracea L. var. sabellica)—a polyphenol-rich plant. Afterwards, high-performance liquid chromatography-mass spectrometry (LC-ESI-MS/MS) and antioxidant activity analyses of snack extracts were performed. In the corn snacks enriched with kale, fifteen phenolic acids were indicated. These were protocatechuic, 4-OH-benzoic, vanillic, trans-caffeic, cis-caffeic, trans-p-coumaric, cis-p-coumaric, trans-ferulic, cis-ferulic, salicylic, gentisic, syringic, 3-OH-cinnamic, trans-sinapic, and cis-sinapic acids. Both the qualitative and quantitative content of polyphenols increased with the addition of B. oleracea. Data from spectrophotometric analyses of the samples showed high DPPH radical scavenging potential of snacks enriched with 4, 6, and 8% of kale. Snacks enriched with kale contain high level of phenolic acids and, therefore, have great potential to make a valuable source of natural antioxidants. High-temperature short-time extrusion-cooking process had no negative impact on polyphenol's activity.


Introduction
Prohealth food (functional food and medical food) contains speci c components which have positive in uence on the health and well-being of the consumer. e concept of "functional food" originated in the early 1980s in Japan [1]. Food could be considered as functional if it provides disease prevention and health promotion against one or more illnesses apart from its nutritional function [2,3]. is kind of victuals may contain a wide range of functional ingredients, such as vitamins, mineral supplement, herbs, phytochemicals, and probiotics, that possess positive physiological effects [4,5]. ese components can be added to, naturally enhanced, or modi ed in a food, because of their diverse health bene ts, such as antioxidant, anti-in ammatory, cardioprotective, antidiabetic, and anticancer activities [3].
Secondary metabolites from plants as bioactive compounds impact on health. Among them, polyphenols have been extensively investigated during the last years [6][7][8]. Many results have indicated that an increased intake of polyphenols may reduce the risk of cardiovascular diseases and type 2 diabetes [9]. ese results were obtained from experiments conducted in animal models with physiologically realistic levels of isolated phenolic compounds [10,11] and in humans consuming polyphenol-rich foods [12]. e consumption of products abundant in polyphenols has been shown as positively in uencing on many risk factors for cardiovascular diseases (CVDs), such as low-density lipoprotein (LDL) cholesterol, blood pressure (BP), and endothelial function [13][14][15].
It is well accepted that fruits and vegetables are important components of a healthy diet and that their consumption helps to prevent a wide range of diseases [16][17][18].
ese bene cial properties have been associated with the presence of bioactive compounds [19,20]. Curly kale (Brassica oleracea L. var. sabellica) is a traditional crop which has regained attention due to the increased focus on Nordic food, as well as its health-related potential on account of high concentrations of phytochemicals, such as polyphenols and vitamins [21][22][23], and to have high antioxidant capacity [24,25]. e growing burden of CVDs, especially in third world countries, has raised interest for new food sources rich in phenolic compounds. In order to introduce new processes that would contribute to the development of functional foods for improving the health of the general population, numerous investigations were performed over the last decade. Nowadays, researchers develop "new" or "emerging technologies" which modify functional properties of food as well as help intensify existing processes. One of the most interesting techniques of functional food production is HTST (high-temperature short-time) extrusion-cooking [26,27]. erefore, the objective of the study reported in presented paper was production of extruded corn snacks supplemented with addition of 2, 4, 6, and 8% of kale (Brassica oleracea L. var. sabellica)-a polyphenol-rich plant. Afterwards, high-performance liquid chromatography-mass spectrometry (LC-ESI-MS/MS) and antioxidant activity analyses of snack extracts were performed.

Chemicals and Plant
Material. Standards of phenolic acids were purchased from Sigma-Aldrich Fine Chemicals (St. Louis, MO, USA). All the chemicals were of analytical grade. LC grade methanol (MeOH) was purchased from J.T. Baker (Phillipsburg, USA). LC grade water was prepared using a Millipore Direct-Q3 puri cation system (Bedford, MA, USA). Brassica oleracea L. var. sabellica (kale) was purchased from "Klasa" company (Kurów, Poland). e plant was dried in the convection dryer at an average temperature of 39.0°C. e dry plant material was milled to particle size lower than 500 μm and sieved.

Extrusion-Cooking
Procedure. Snacks enriched with kale were produced rst time in the Department of Food Process Engineering, University of Life Sciences in Lublin. Corn was used as a control. Blends of corn grits and ground kale were prepared by mixing dry components by replacing corn with 2, 4, 6, and 8% of kale. e samples were conditioned to 15% moisture by spraying with water and mixing continuously for 10 minutes. Blends were processed in a TS-45 single screw extrusion-cooker with L/D � 12 (ZMCh Metalchem, Gliwice, Poland), according to the previously described method [28]. Snacks were ground (bellow 500 μm) and stored in a dark place in closed plastic bags before tests.

Extraction Procedures.
Ultrasound-assisted extraction (UAE) was carried out in an ultrasonic bath (J.P. Selecta, Barcelona, Spain; frequency 20 kHz, power 100 W) with a thermostat. Extraction was performed with 2 g of sample at a temperature of 60°C with 40 mL of solvent in each cycle, by three cycles for 10 min (30 min, previously optimized). Four di erent solvent systems (ethanol, methanol, 80% aqueous ethanol, and 80% aqueous methanol) were evaluated for the extraction of phenolic acids. e highest yields of all analyzed compounds gave ethanol. After evaporation of the extracts, the residues were dissolved in methanol (10 mL). e whole procedure was repeated three times for each sample [29]. Before chromatographic analysis, the extracts were ltered through a 0.45 μm nylon syringe lter.

LC-ESI-MS/MS Analysis of Phenolic Compounds.
According to the method described previously [29], the samples were analyzed by high-performance liquid chromatography and electrospray ionization mass spectrometry (HPLC-ESI-MS/MS). Analysis was performed using Agilent 1200 Series HPLC (Agilent Technologies, USA) equipped with a binary gradient solvent pump, a degasser, an autosampler, and a column oven. Phenolic acids were separated at 25°C, on Zorbax SB-C18 column (2.1 ×50 mm, 1.8 µm particle size; Agilent Technologies, USA), using 3 µL injections. MS detection was performed in a 3200 QTRAP mass spectrometer (AB Sciex, USA) equipped with an electrospray ionization source (ESI) and a triple quadrupole-ion trap mass analyzer that was controlled by the Analyst 1.5 software. e QTRAP-MS system was equipped with an electrospray ionization source (ESI) operated in the negative ion mode. e correlation coe cient of all calibration curves was R 2 > 0.9988. Analytical results and parameters of LC-MS/MS quantitative method-data for calibration curves, limit of detection (LOD), and the limit of quanti cation (LOQ) values for each analyzed phenolic acids-are described in the paper presented previously [29].

Radical-Scavenging Activity of the Analyzed Extracts.
e radical-scavenging activity of the extracts was determined spectrophotometrically against the DPPH radical [30]. e concentration of DPPH used for the experiment was 0.1 mM (4 mg of the free radical in 100 mL of methanol). Measurement of reference sample of DPPH solution was performed by mixing 2.0 mL of the solution and 1.0 mL of methanol. Measurement of snack extracts was done after mixing 2.0 mL of DPPH solution and 1.0 mL of the extracts. Each measurement was repeated three times at the wave length of 517 nm at room temperature. e nal result was the average of three replicates. e antioxidant activity was calculated with the following formula [31]: where A 0 is the absorbance of the reference sample and A 1 is the absorbance of the sample with tested extracts.

Results and Discussion
e well-known correlation between nutrition and ne fettle demonstrates the great possibilities of food to maintain or improve people's health. Extremely important point of the research is the extraction and characterization of natural components (such as polyphenols), with biological activity that can be incorporated into a functional food, contributing to consumer's well-being. Previous studies of the authors have found high content of the phenolic compounds in Brassica oleracea L. var. sabellica (kale) [29]. Taking this fact into account, the authors produced corn snacks enriched with kale using extrusioncooking process and investigated the phenolic acid content and antioxidant properties of these samples. Extrusioncooking, as HTST method, seems to be one of the best methods for obtaining the maximum nutritive value of several plant products [32]. e rst step of the experiment was extraction of polyphenols from snack samples. e most e ective conditions for the isolation of analyzed phenolic acids using UAE were as follows: extraction time 30 min, ultrasound frequency 20 kHz, ultrasound power 100, and ethanol as extractant.
e next point of the study was high-performance liquid chromatography-mass spectrometry analysis of the polyphenolic extracts. In the corn snacks enriched with kale, fteen phenolic acids were indicated (Table 1). ese were protocatechuic, 4-OH-benzoic, vanillic, trans-ca eic, cisca eic, trans-p-coumaric, cis-p-coumaric, trans-ferulic, cis-ferulic, salicylic, gentisic, syringic, 3-OH-cinnamic, trans-sinapic, and cis-sinapic acids. However, concentrations of gentisic and syringic acids were lower than the limit of quanti cation (LOQ) but higher than the limit of detection (LOD). Snacks without additives contained only eight phenolic acids: 4-OH-benzoic, trans-p-coumaric, cisp-coumaric, trans-ferulic, cis-ferulic, salicylic, trans-sinapic and cis-sinapic acids. Yields of the following acids were lower than the limit of quanti cation (LOQ) but higher than RSD% � relative standard deviation (n � 3); BQL � peak detected, concentration lower than the LOQ but higher than the LOD.     the limit of detection (LOD): 4-OH-benzoic, trans-sinapic, and cis-sinapic. Both the qualitative and quantitative contents of polyphenols increased with the addition of kale; for example, 3-OH-cinnamic acid occurs only in snacks enriched with 6% of B. oleracea. Exemplary chromatogram of analyzed phenolic acids is presented in Figure 1.
In order to assess the accuracy of the methods, recovery studies were performed. e samples moistened with the solvent were spiked with known amounts of each standard solution (three concentration levels). Afterwards, ultrasound-assisted extraction was carried out using the same ways employed in the quantitative determination of phenolic compounds in the samples. e recoveries were in the range of 89.2% (for vanillic acid) to 97.3% (for salicylic acid).
Data from spectrophotometric analyses of the samples showed high DPPH radical-scavenging potential of snacks enriched with 8, 6, and 4% of kale ( Figure 2). Antioxidant properties of analyzed extracts were positively correlated with the level of B. oleracea in corn snacks. Free radical scavenging ability increased with the addition of kale. e snacks without additives and containing 2% of kale did not scavenge free radical. High antioxidant properties of active samples were observed right now after the rst ve minutes of the experiment, while maximum radical-scavenging activity was observed after 30 min.
Kale contains a complex mixture of health-related phytochemicals including phenolic compounds [33]. e ndings of the research have demonstrated that snacks enriched with kale contain high level of phenolic acids and, therefore, have great potential to be a good source of natural antioxidants. ese products have the potential to reduce the risks of lifestyle diseases, for example, chronic in ammation, cardiovascular diseases, and type 2 diabetes, which have become an epidemic and require concerted e ort in their treatment. e application of dietary therapy in these disorders is one of the options to ght them. e aim of the experiment conducted by Grace et al. was to test the kale phytoactive compounds (e.g., polyphenols) complexed with proteins as bioactive food ingredients [34]. Bioforti ed Max. 6.1e4 cps.
Max. 2.9e5 cps.  matrices, created with kale, bound to edible proteins were tested for their ability to inhibit biomarkers of acute and chronic in ammation [35]. e matrices demonstrated nutritional and sensory attributes amenable to the use as food component, while delivering anti-in ammatory bene ts expected from kale consumption, while delivering antiin ammatory bene ts expected from kale consumption. Results of anti-in ammatory bioassays demonstrated that kale-forti ed matrices, at reasonable dietary levels, suppressed the activation of key proin ammatory genes triggered by an in ammatory stimulus [34].
Our results show that starch-based products with high quantity of polyphenols may be produced by extrusioncooking, because this high-temperature short-time process had no negative impact on polyphenol's activity. ese ndings are in accordance with the work of Anton et al. [36], who observed a signi cant increase in total polyphenol content and antioxidant activity of extruded snacks obtained from blends of corn starch and navy/small red beans. Korus et al. [37] studied the e ect of extrusion on several polyphenols in raw and extruded beans. Interestingly, they observed an increase of 14% in the amount of phenolics in extrudates compared to raw dark-red beans. e overall increase of 14% observed in dark-red beans during extrusion was mainly due to an increase in quercetin (by 84%) and ferulic acid (by 40%) along with a decrease in chlorogenic and ca eic acids by 33 and 9%, respectively. us, extrusion cooking of whole bean our is advantageous to obtain products with higher levels of polyphenols, provided products poses sensorial acceptance [38]. Other authors [39] reported a signi cant increase in free/bound phenolic acids such as syringic, ferulic, and coumaric, except vanillic acid during extrusion of buckwheat (Fagopyrum esculentum Moench L.). e observed increase in these phenolic compounds could be due to the increased release of these bioactive compounds from the matrix due to extrusion, thus accessible in the extraction.
In conclusion, the bene ts associated with regular dietary consumption of phytochemically rich food, for example, corn snacks enriched with kale, contribute to reduced chronic in ammation and can provide anti-infective and other health bene ts.

Conclusions
In the corn snacks enriched with kale, fteen phenolic acids were indicated. e results of the above study indicate that snacks enriched with kale prepared by the extrusion-cooking process have great potential to be a good source of natural antioxidants with substantiated health bene ts, especially when 4% or more was applied.
ese products have the potential to reduce the risks of lifestyle diseases, for example, cardiovascular diseases and type 2 diabetes. HTST extrusioncooking processing of snacks had no negative impact on the antioxidant activity of phenolic acids.  Figure 2: Free radical scavenging activity of extracts of corn snacks (S). Snacks enriched with 2, 4, 6, and 8% kale (S + 2%, S + 4%, S + 6%, and S + 8%, resp.) towards DPPH in methanol.