Journal of BotanyVolume 2014 (2014), Article ID 828750, 6 pageshttp://dx.doi.org/10.1155/2014/828750

Research Article

Effect of NPK and Poultry Manure on Growth, Yield, and Proximate Composition of Three Amaranths

Stephen Oyedeji,¹ David Adedayo Animasaun,¹ Abdullahi Ajibola Bello,¹ and Oludare Oladipo Agboola²

¹Department of Plant Biology, University of Ilorin, PMB 1515, Ilorin 240003, Nigeria²Biology Department, The Polytechnic Ibadan, PMB 22, Ibadan 200284, Nigeria

Received 1 October 2013; Accepted 3 December 2013; Published 12 January 2014

Academic Editor: Muhammad Y. Ashraf

Copyright © 2014 Stephen Oyedeji et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract.

The study compares the growth, yield, and proximate composition of Amaranthus hybridus, Amaranthus cruentus, and Amaranthus deflexus, grown with poultry manure and NPK in relation to the unfertilized soil of Ilorin, Nigeria. Viable seeds of the Amaranths raised in nursery for two weeks were transplanted (one plant per pot) into unfertilized soil (control) and soils fertilized with either NPK or poultry manure (PM) at 30 Kg ha⁻¹ rate arranged in randomized complete block design with four replicates. Data were collected on plant height, stem girth, number of leaves, leaf area, and number of branches from 1 week after transplanting (1 WAT). Fresh weight, dry weight, and proximate composition were determined at 6 WAT. Except for the length, breadth, and number of leaves, the order of growth parameters and yield in the three Amaranthus species was NPK > PM > control. NPK grown Amaranthus species had the highest protein while PM-grown vegetables had the highest ash content. Crude fibre in A. cruentus grown with PM was significantly higher than NPK and the control. The NPK treatment of A. hybridus and A. deflexus had the highest crude fibre content. NPK and PM favoured growth and yield of the Amaranthus species but influenced proximate composition differently.

1. Introduction

In Nigeria, as in most other tropical countries of Africa where the daily diet is dominated by starchy staple foods, vegetables are the cheapest and most readily available sources of important proteins, vitamins minerals, and essential amino acids [1]. Of all vegetables, Talinum triangulare, Telfaria occidentalis, Corchorus olitorius, Vernonia amygdalina, and Amaranthus species such as A. hybridus, A. cruentus, A. caudatus, and A. deflexus are mostly consumed. The production and nutritional values of these vegetables are limited due to the low fertility of native soils in most parts of Nigeria [2]. The use of inorganic fertilizer to increase yield has been found to be effective only within few years, demanding consistent use on long-term basis [3]. The hazardous environmental consequences and high cost of inorganic fertilizers make them not only undesirable but also uneconomical and out of reach of the poor farmers who still dominate the Nigerian agricultural sector [4]. This has led to increased use of organic manure, a readily available alternative, which proves more environmentally friendly.

In recent times, attention has been directed towards organic manure because of the rising cost of inorganic fertilizers coupled with their inability to give the soil the desired sound health. Poultry manure, sometimes called chicken manure, is an excellent soil amendment that provides nutrients for growing crops and also improves soil quality when applied wisely, because it has high organic matter content combined with available nutrients for plant growth [5]. The chemical composition of poultry manure varies with factors such as source of manure, feed of the birds, age and condition of the birds, storage, handling of manure, and litter used [6]. Poultry waste consists of droppings, wasted feed, broken eggs, feathers, and sometimes sawdust from poultry floor. It also includes the dead birds and hatchery waste, all of which are high in protein and contain substantial amount of calcium and phosphorus due to high level of mineral supplement in their diet. Poultry manure has been reported to contain more plant nutrients than all other organic manures [7].

At present, vegetable farmers mostly apply poultry manure in combination with inorganic nitrogen-based fertilizers such as Urea and NPK [8], often because poultry manure alone is believed to dissolve slowly and may not meet up the yield of vegetables. There is a need to determine the independent influence of poultry manure and inorganic nitrogen fertilizer such as NPK on the growth, yield, and nutritional quality of fast-growing vegetables like Amaranthus species as to justify the continuous mixture of both or otherwise. The present study compares the growth, yield, and proximate composition of three Amaranthus species—A. hybridus, A. cruentus, and A. deflexus, grown with poultry manure, NPK in relation to the unfertilized (control) soil.

2. Materials and Methods

The potted experiment was set up in a screen house in 2013 at the University of Ilorin (N 08° 28′ 53.3′′, E 04° 40′ 28.9′′), Ilorin, Nigeria which lies in the southern guinea savanna belt of Nigeria. The annual rainfall in the area is about 1200 mm and temperature varies between 33°C and 34°C during the year, with a distinct dry season from December to March. The effects of inorganic fertilizer (NPK—15 : 15 : 15) and organic fertilizer (poultry manure) were tested on growth, yield, and proximate composition of three species of Amaranthus—A. cruentus, A. hybridus, and A. deflexus. Viable seeds of the three species were obtained from the National Centre Genetic Research and Biotechnology, Moor Plantation, Ibadan, Nigeria. Seed viability testing was determined using floatation method [9]. The seeds were raised in nursery for two weeks and one plant per pot (0.30 m by 0.38 m diameter by depth) was transplanted into the unfertilized soil (control) and soils fertilized with either NPK or poultry manure (PM) at 30 kg·ha⁻¹ rate incorporated into 12 kg top soil. The treatments were arranged in a randomized complete block design with four replicates. Weeds were handpicked and irrigation was done regularly at the same rate. Data collection on plant height, stem girth, number of leaves, leaf area, and number of branches was started 1 week after transplanting. The plants were harvested 6 weeks after transplanting and weighed fresh and dried. The proximate composition of moisture, protein, ash, fibre, and carbohydrate content was determined. The moisture content determination was done according to the method described by [10]. The crude protein, calculated from nitrogen, was determined by micro-Kjeldahl procedure of digestion, distillation, and titration according to AOAC procedure [11]. The ash content was expressed as the percentage weight of ash produced to original weight of dried sample dry ash in muffle furnace at 600°C [10]. Crude fibre determination was carried out on 2 g fat-free samples obtained after crude fat determination using Soxhlet procedure [12]. Total carbohydrate was calculated as the difference between 100 and the sum of the moisture, protein, fat, fibre, and ash contents [13]. Analysis of variance was performed on the data and means were separated using Duncan’s multiple range test.

3. Results

The inorganic (NPK) and organic fertilizer (PM) generally increased the growth of all the species of Amaranthus in this study. This was revealed by the growth and luxuriance of the three Amaranthus species planted with fertilizers in the weeks after transplanting (WAT). However, NPK-grown A. hybridus and A. cruentus had fewer leaves than PM and the control. The NPK treatment of A. deflexus was significantly lower than the control only at 2 and 3 WAP (Table 1). Leaf length in the NPK-grown A. hybridus and A. cruentus was initially shorter than that in PM and control (1 WAT and 2 WAT) but it had the longest leaves at 6 WAT. A. deflexus grown with NPK had the longest leaves except at 2 and 3 WAP (Table 2). Results of leaf breadth in the three species were similar to those of leaf length as A. cruentus in the NPK-fertilized soil was initially lower than that in PM and control (at 2 WAT) but higher at later weeks (Table 3). Of the three treatments, plant height of A. hybridus in NPK-treated soil was shortest from 1 WAT to 3 WAT, but this difference was only significant at 3 WAT. A. cruentus in NPK- and PM-fertilized soils was significantly shorter than that of the control at 2 WAT but taller at 6 WAT. A. deflexus in the soil amended with NPK was shortest at 6 WAT (Table 4). The slow response of plants in the NPK-treated soil during the initial stage of the experiment may have influenced the stem girth of A. hybridus and A. cruentus at 1-2 WAT and 1 WAT, respectively, which had slimmer girths. A. deflexus in PM-treated soil had slimmer girths when compared with the control plants from 1 WAT to 3 WAT (Table 5). Only A. hybridus in NPK-treated soil had branches at 3 WAT, and the number was significantly different from PM and the control at 6 WAT. Only A. deflexus had branches at 2 WAT, with the NPK treatment having the significantly highest number of branches at 2 WAT and 3 WAT (Table 6). The weights (fresh and dry) of plants in the NPK-treated soil were significantly higher than PM and the control in all three species. The order of fresh weight and dry weight was similar in all the three Amaranthus species. A. hybridus had the highest fresh and dry weights of  g and  g, respectively (Figure 1).

Table 1: Number of leaves in three Amaranthus species grown in fertilized and unfertilized soil from 1 to 6 weeks after transplanting.

Amaranth
Treatments
Number of leaves

1 WAT
2 WAT
3 WAT
4 WAT
5 WAT
6 WAT

A. hybridus
Control
6ab
15a
19a
27a
23a
25a

PM
7a
18a
22a
30a
28a
25a

NPK
4b
7b
16a
27a
24a
21a

A. cruentus
Control
8a
16ab
28a
34a
31a
28a

PM
8a
22a
28a
35a
34a
31a

NPK
6a
12b
28a
36a
32a
32a

A. deflexus
Control
9a
36a
44a
49a
54a
57a

PM
7a
18a
29b
37a
41a
39b

NPK
10a
24b
36b
44a
53a
59a

Comparison is treatment-wise. Means with the same letter(s) within each species are not significant at .

Table 2: Leaf length in three Amaranthus species grown in fertilized and unfertilized soil from 1 to 6 weeks after transplanting.

Amaranth
Treatments
Leaf length (mm)

1 WAT
2 WAT
3 WAT
4 WAT
5 WAT
6 WAT

A. hybridus
Control
3.73a
9.30ab
12.60a
15.80b
16.23b
16.66b

PM
4.60a
11.40a
14.56a
15.50b
16.16b
16.40b

NPK
3.13a
5.46b
12.46a
24.00a
27.20a
28.36a

A. cruentus
Control
3.66a
8.83ab
12.26a
12.93b
13.53b
13.93b

PM
4.30a
10.66a
12.70a
14.00b
14.73b
15.06b

NPK
2.90a
6.73b
14.73a
18.36a
19.00a
20.10a

A. deflexus
Control
4.00a
10.63a
12.53a
13.83a
14.20a
14.16b

PM
2.80b
8.03b
11.46a
12.56a
13.26a
13.43b

NPK
4.16a
10.30a
11.50a
14.26a
15.53a
16.83a

Comparison is treatment-wise. Means with the same letter(s) within each species are not significant at .

Table 3: Leaf breadth in three Amaranthus species grown in fertilized and unfertilized soil from 1 to 6 weeks after transplanting.

Amaranth
Treatments
Leaf breadth (mm)

1 WAT
2 WAT
3 WAT
4 WAT
5 WAT
6 WAT

A. hybridus
Control
1.50a
4.90ab
6.63a
7.53b
7.96b
8.13b

PM
2.10a
6.00a
7.16a
7.90b
8.20b
8.43b

NPK
1.50a
2.56b
7.63a
11.33a
13.13a
14.03a

A. cruentus
Control
1.46a
4.33ab
6.16a
6.36b
6.66b
6.53b

PM
1.90a
5.33a
6.20a
6.93b
7.23b
6.90b

NPK
1.30a
3.33b
7.60a
8.76a
9.16a
9.60a

A. deflexus
Control
1.76a
2.93b
3.43a
3.83b
4.30a
3.73b

PM
1.23b
2.63b
3.60a
3.83b
4.06a
3.50b

NPK
1.83a
3.86a
4.16a
4.93a
5.23a
4.80a

Comparison is treatment-wise. Means with the same letter(s) within each species are not significant at .

Table 4: The plant height of three Amaranthus species grown in fertilized and unfertilized soil from 1 to 6 weeks after transplanting.

Amaranth
Treatments
Plant height (cm)

1 WAT
2 WAT
3 WAT
4 WAT
5 WAT
6 WAT

A. hybridus
Control
9.70a
16.53a
29.36ab
42.96a
53.00b
64.96b

PM
11.00a
24.66a
38.30a
53.73a
65.73ab
79.13b

NPK
7.60a
9.80a
24.03b
58.90a
79.93b
108.50a

A. cruentus
Control
7.30a
16.66a
34.30a
46.03b
59.36a
74.43b

PM
9.20a
5.83b
37.70a
55.00ab
70.33a
86.83ab

NPK
7.90a
10.30b
39.33a
65.23a
77.60a
100.66a

A. deflexus
Control
5.93a
10.13a
29.43a
61.20a
89.20a
106.00a

PM
4.93b
5.96b
21.16b
49.93a
73.26a
110.03a

NPK
6.00a
10.36a
23.70ab
51.16a
67.26a
84.66b

Comparison is treatment-wise. Means with the same letter(s) within each species are not significant at .

Table 5: Stem girths of three Amaranthus species grown in fertilized and unfertilized from 1 to 6 weeks after transplanting.

Amaranth
Treatments
Stem girth (mm)

1 WAT
2 WAT
3 WAT
4 WAT
5 WAT
6 WAT

A. hybridus
Control
2.06a
5.15ab
7.50a
10.63b
12.74b
13.67b

PM
2.27a
7.49a
9.84a
11.70b
12.46b
13.21b

NPK
1.66a
2.35b
8.77a
17.40a
22.27a
25.36a

A. cruentus
Control
1.91a
5.66a
7.06a
8.71b
10.19b
11.01b

PM
2.65a
3.63b
8.50a
9.67b
11.75b
13.38b

NPK
1.76a
4.98a
10.78a
17.33a
23.36a
27.38a

A. deflexus
Control
2.21a
5.66a
7.39a
9.14b
9.71b
10.54b

PM
1.56b
3.63b
7.09a
10.07ab
10.77ab
11.35ab

NPK
2.23a
4.98a
8.09a
10.75a
11.56a
12.00a

Comparison is treatment-wise. Means with the same letter(s) within each species are not significant at .

Table 6: Number of branches in three Amaranthus species grown in fertilized and unfertilized soil from 1 to 6 weeks after transplanting.

Amaranth
Treatments
Number of branches

1 WAT
2 WAT
3 WAT
4 WAT
5 WAT
6 WAT

A. hybridus
Control
0
0
0b
5b
8b
11b

PM
0
0
0b
8b
12b
16b

NPK
0
0
11a
20a
23a
26a

A. cruentus
Control
0
0
4b
9c
13b
16b

PM
0
0
10a
16b
19b
19b

NPK
0
0
14a
23a
29a
32a

A. deflexus
Control
0
7b
17b
28a
30a
31a

PM
0
5b
18ab
25a
28a
30a

NPK
0
10a
20a
28a
34a
38a

Comparison is treatment-wise. Means with the same letter(s) within each species are not significant at .

Figure 1: Fresh and dry weights of three amaranths grown in fertilized and unfertilized soil.

The proximate composition in all three species of Amaranthus due to the soil amendments varied for all parameter measured. Except for A. hybridus, protein content in the species grown in NPK-treated soil was significantly higher than that of other treatments, while plants in PM-treated soils had the highest ash content. Crude fibre in A. cruentus grown with PM was significantly higher than NPK and the control, contrary to that in A. hybridus and A. deflexus with the NPK treatment having the highest crude fibre. A. hybridus and A. cruentus grown with NPK had the higher lipid content than PM and the control, but A. deflexus from PM-treated soil had the highest lipid content. Except for A. hybridus, the carbohydrate contents in the Amaranthus grown with NPK were significantly lower than the control and PM. The moisture contents in these plants were also significantly lower than the control (Table 7). Generally, the protein and fibre contents in A. hybridus were significantly higher than those in A. cruentus and A. deflexus. But ash, lipid, and carbohydrate contents in all three species as well as the moisture content in A. cruentus and A. deflexus were at par (Figure 2).

Table 7: Proximate composition of three species of Amaranthus grown in control and fertilized soils (PM and NPK).

Species
Treatment
% Protein
% Ash
% Crude fibre
% Lipid
% Carbohydrate
% Moisture

A. hybridus
Control

PM

NPK

A. cruentus
Control

PM

NPK

A. deflexus
Control

PM

NPK

Comparison is treatment-wise. Means with the same letter(s) within each species are not significant at 5.

Figure 2: Proximate composition of A. hybridus, A. cruentus, and A. deflexus.

4. Discussion

The slower growth in plants in NPK- and PM-fertilized soil during the early weeks after transplanting could be due to initial physiological changes associated with transplanting. This initial drawback after transplanting have been reported for tomato [14], rice [15], maize [16], and sorghum [17]. This effect was outweighed during the later weeks after transplanting (4–6 WAT) as N, P, and K release after mineralization significantly enhanced the growth parameters (leaf number, length and breadth, plant height, branching, and stem girth) in the three Amaranthus species. The higher performance of crops in NPK over PM is indicative of the ease of dissolution of nutrients in the inorganic fertilizer being in a more soluble form. Poultry manure also contains useful soil nutrients that are needed for the growth of plants [18], but their composition is in the crude form that is released slowly to the soil [19]. This possibly accounted for slower growth of plants in PM-treated soil to those of NPK and its higher performance than the control. This influence on growth by NPK and PM possibly resulted in higher yield than in the control. This result corroborates those of [4, 20, 21] on growth of Amaranthus and that of [22] on yield of Amaranths.

The higher protein in the Amaranthus species grown with NPK and PM is consistent with the result of [22]. The increased nitrogen to the soil by the fertilizers increased the uptake by the Amaranthus resulting in increased protein. The ash content in PM plants was significantly higher possibly because of the balanced nutrient in the manure, unlike NPK with proportionate contents of N, P, and K and the control with lower concentration of nutrients. The significantly higher carbohydrate in the control plants of A. cruentus and A. deflexus also corroborates with those of [22]. Except for moisture content, the protein, fibre, fat, and carbohydrate composition in the three species were higher than those reported by [23]. The nutritional content of A. cruentus except for lipid was lower than that reported by [24]. Of the three species, only A. hybridus is considered a good source of protein as it accounts for more than 12% of its nutritional value [25]. This is contrary to the report by [1] in which A. cruentus was implicated as a rich source of protein accounting for 11.4%–14.1% of the proximate composition.

5. Conclusion

Except for the length, breadth, and number of leaves, growth parameters and yield were in the order NPK > PM > control. Amaranthus species grown with NPK significantly had higher protein, while those grown with PM had higher ash content. A. hybridus had the highest protein composition and could be considered a good source of vegetable protein when consumed. Both inorganic fertilizer (NPK) and organic (PM) increase soil fertility, but plants grown with inorganic fertilizer performed better. Since the Amaranthus grown with NPK and PM had better growth but differs in nutritional quality, the choice of the nutrient may influence determining the choice of manure to encourage.

Conflict of Interests

The authors declare that there is no conflict of interests regarding the publication of this paper.

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