Multiresidue Analysis of 86 Pesticides Using Gas Chromatography Mass Spectrometry : II-Nonleafy Vegetables

A total of 1057 samples of fresh vegetables from import and domestic production were analyzed (cold pepper, egg plant, carrot, cucumber, potato, hot pepper, cultivation tomato, squash, beans, okra, onions, cauli�ower, and green house tomato). e aim of this study was to investigate pesticide residues in market foods in Riyadh, which have been collected from Riyadh Development Company (Al-Tamer Vegetables Market). Pesticide residues were determined by gas chromatography with mass selective detector (GC-MSD). Amultiresidue method was developed and described for simultaneous determination of 86 pesticides commonly used in crop protection.is method used to determine 86 pesticide residues with a broad range of physicochemical properties in fresh vegetables related to organophosphorus (OPP), organochlorines (OCP), pyrethroids, and carbamates mainly used in agriculture. Sample extract was cleaned up by using AOACmethod. Pesticide residues above the maximum residue limits (MRL) were detected in 15.89% of the total samples (168 from 1057 samples), but 83.90% of the total samples (887 from 1057 samples) has no residues or contained pesticide residues at or below MRL. e detected and most frequently found pesticide residues were permethrin (45 times) and endosulfan (34 times) followed by deltamethrin (27 times). e �ndings of this study pointed to the following recommendations: the need for a monitoring program for pesticide residues in imported food crops.


Introduction
Fresh vegetables are an important part of a healthy diet as they are a signi�cant source of vitamins and minerals. However, fresh vegetables can also be a source of noxious toxic substances-pesticides. Vegetables are traded worldwide and the list of pesticides that might have been applied in their agricultural production is usually not known [1][2][3].
Pesticides constitute a very important group of chemical compounds that have to be controlled due to their high toxicity and their widespread use in agricultural practice for �eld and postharvest protection. e presence of pesticide residues in food is a direct result of pesticide use on crops. Over 1000 compounds may be applied to agricultural crops in order to control undesirable moulds, insects, and weeds [4]. e pesticide residues causing food contamination have become increasingly frequent in recent years raising question about their human health and economic consequences [5]. Widespread contamination of water, air, and soil by chemicals and industrial pollutants means that the crops that we grow and the animals we use for food are oen exposed to toxic substances [6].
e levels of pesticide residues in foodstuffs are generally legislated so as to minimize the exposure of the consumer to harmful or unnecessary intakes of pesticides, to ensure the proper use of pesticides in terms of granted authorization and registration (application rates and preharvested intervals), and to permit the free circulation of pesticide-treated products, as long as they comply with the �xed maximum residue limits (MRLs). MRL for pesticide residues represents the maximum concentration of that residue (expressed in mg/kg) that is legally permitted in speci�c food items. e establishment of MRL is based on good agricultural practice data on food derived from commodities. MRLs are not toxicological limits, but they must be toxicologically acceptable. Exceeded MRLs are strong indicators of violations of good agricultural practices [7][8][9].
A multiresidue method is described for determination of 86 pesticides commonly used in crop protection. Good sensitivity and selectivity of the method are obtained with limits of quanti�cation 0.01 mg/kg in almost all cases. e method was applied very satisfactorily to routine analysis as a complement to traditional GC-MS method and �nally, limit of detection was also 10-20 times lower than maximum residue levels (MRL) established by codex alimentarius commission. More than 500 samples from leafy vegetables have been collected from vegetables market (Riyadh Development Company) in Riyadh. Pesticide residues were detected in 24.69% of the total samples (140 from 567 samples) and pesticides concentration was higher than MRL in 104 samples [10].
e objective of this study was to investigate the presence of pesticide residues in nonleafy vegetables in food market in Saudi Arabia. Samples were collected from the central market located in Riyadh (Al-Tamer Vegetables Market), during two years, that is, 2007-2008. Collected data are to be used as a reference point for future monitoring and taking preventive measures to minimize human health risks.

Reagents and Equipments.
All pesticide standards were obtained from (Riedel de Haen and Supelco). We prepared 1 mg/mL stock solution of each by dissolving 20 mg of the pure analytical standard in 20 mL of acetone. A single composite standard solution was prepared by diluting with acetone according to limit of detection (LOD). All standard solutions were stored in glass-stoppered �asks at 4 ○ C. Mixed compound calibration solutions were prepared in acetone and they were used as spiking solution. Solvents (residue analysis grade) used were acetone, acetonitrile, petroleum ether, and other reagents such as sodium chloride and anhydrous sodium sulphate� �orisil 60-100 mesh for residue analysis was also purchased from (Fluka). e �orisil (activated magnesium silicate used as adsorbent) and anhydrous sodium sulphate were activated at 100 ○ C over night and stored in 500 mL glass �ask with glass stoppers and stored in oven at 100 ○ C. e equipments used included a high-speed blender with a stainless steel jar (waring, USA), a shaking separation �nal (GFL, Germany), a rotary evaporator, R 215 and cooler circulator chiller B-740 (Buchi, Switzerland), Buchner funnel and chromatographic tubes with Te�on stopcocks and course fritted glass (Agilent, USA), and syringes (Hamilton Bonadus AG, Switzerland). All glassware were rinsed thoroughly using soap and deionization water, then washed with acetone and dried in oven (100-130 ○ C) over night 2.2. Samples Collection. is study was conducted during two years from January 2007 to December 2008. More than 500 nonleafy vegetable samples (cold pepper, egg plant, carrot, cucumber, potato, hot pepper, cultivation tomato, squash, beans, okra, onions, cauli�ower, and green house tomato) have been collected from Al-Tamer Vegetables Market in Riyadh, Saudi Arabia. Samples were put in sterile polythene bags and transported to the laboratory where they were analyzed immediately or stored at 4 ○ C until analysis within 24 h.

Sample Preparation, Extraction, and
Partitioning. e chopped samples of raw nonleafy vegetable (120 g) were placed in a stainless steel jar 1L and extracted with 200 mL of acetonitrile and 10 g celite, the blender was vigorously homogenized into high speed for 2 min, and the mixture was �ltrated by using Buchner funnel �tted with shark-skin �lter paper into 500 mL suction �ask (Buchi, Switzerland). An aliquot of organic was transferred to 1L separator funnel and added 100 mL of petroleum ether (PE) and the mixture was vigorously shaken for 1-2 min and then was added 100 ml saturated solution of NaCl and 600 water. e mixture was vigorously mixed and the separator funnel was allowed to be held at horizontal position for few minutes. e aqueous layer was discarded and the solvent layer was washed with twice time 100 mL portions of distilled water and the washed layer was transferred into 100 mL beaker and washed with 15 g of anhydrous sodium sulphate. Finally, the extract was concentrated to 5 mL volume and transferred directly to �orisil column ( = 3).

Minimum Detection Limit (MDL).
To determine the maximum residue limits (MRL), retention time (Rt), limit of quanti�cation (LO�), ion target, quali�er ions / by scan mode, and perform the GC-MS quanti�cation ( Table 1) by using a four-point calibration curve plotting peak area versus mg l −1 concentration of 86 pesticides using the dilution levels ranged from 0.0001 to 5.00 mg l −1 .

Florisil Column
Cleanup. Florisil column cleanup was conducted according to the AOAC [11]. e column was peppered containing about 12 cm activated �orisil topped with 1 cm anhydrous sodium sulphate, and column was washed by 40 mL petroleum ether (PE) and was added extract concentrated to 5 mL and was allowed to pass through the column. e walls of the tube were rinsed additional small portions of petroleum ether and elute at 5 mL/min with 200 mL 6% eluting solvent (diethyl ether in petroleum ether) (PE), and then 200 mL 15% and �nally 200 ml 50% eluting solvent (diethyl ether in PE) at 5 mL/min.

2.�. C�romatograp�ic �nstrumentation and �uanti�cation.
Gas chromatograph-mass spectrometer (Aglient model 6890N) gas chromatograph coupled with (model 5975B) quadrupole mass spectrometer with a GC column HP-5MS 5% phenyl and 95% methyl siloxane, 30 m × 0.25 mm id × 0.25 m �lm thickness. GC operating conditions� splitless injection, injector temperature 250 ○ C, helium carrier gas (99.9999 purity) at �ow rate 0.9 mL/min with column head pressure 7.4 psi, oven temperature from 70 ○ C (2 min hold), then raised to 130 ○ C at the rate (25 ○ C/min) aerwards raised to 220 ○ C at (2 ○ C/min) and then raised to 280 ○ C at (10 ○ C/min) and eventually (4.6 min hold). e sample (1 L) was injected in split less modes. e MS system was routinely set in selective ion monitoring (SIM) mode and each compound was quantitated based on peak area using one target and one or two quali�er ion. Mass spectrometer parameter was set as follows: electron impact ionization mode with 70 eV electron energy and scan mass range 100-400 at 0.62 sec/cycle. Ion source temperature 230 ○ C, MS quad temperature 150 ○ C, EM voltage 1450, and solvent delay 4 in.

Results and Discussion
A multiresidue procedure was carried out to monitor the pesticide residues in a wide range of the most common consumed non leafy vegetables samples collected during two years, that is, 2007-2008. e analyzed samples composed of thirteen species of non leafy vegetables, that is, hot pepper, bell pepper, onions, potato, cucumber, okra, bean, carrot, cultivation tomato, green house tomato, squash, egg plant, and cauli�ower. A wide range of pesticide residues were detected and quanti�ed in the analyzed samples during the two years of this study. In 2007, it was detected the residues of 31 pesticides while in 2008 it was detected the residues of 28 pesticides.

Pesticide Residues in Nonleafy Vegetables during the
Year of 2007. Data in Table (2) shows the amounts of the detected pesticide residues in non leafy vegetable samples from different farms and locations collected from Al-tamer vegetables market in Riyadh, Saudi Arabia during the year of 2007. According to the detected pesticides, it is clear that there are a wide range of compounds which included insecticides (31 compounds), herbicides (four compounds, that is, linuron, simazine, alachlor, and aldrin), and fungicides (one compound, that is, tetradifon). e detected insecticide residues which represent the majority of the detected compounds, it was found that such insecticides could be classi�ed chemically into their major four chemical groups, that is, organochlorines, organophosphorus, pyrethroids, and carbamates. e detected organochlorines substances were six insecticides, that is, chloroneb, lindane, endosulfan, dicloran, mirex, and P,P-DDD while the organophosphorus insecticides included nine agents, that is, chlorpyrifos, dimethoate, malathion, mevinphos, disulfoton, phosmet, ethoprophos, diazinon and iprobenfos. As for pyrethroids, it was detected of seven compounds included permethrin, deltamethrin, cypermethrin, -cyhalothrin, cy�uthrin, fenvalerate, and resmethrin. For carbamates, only three compounds were detected, that is, carbaryl, bendiocarb, and thiobencarb. e mentioned pesticides were detected in thirteen non leafy vegetables, included hot pepper, cold pepper, onions, potato, cucumber, okra, bean, carrot, and cultivation tomato, green house tomato, squash, egg plant, cauli�ower. According to the detected pesticides in and/or on the non leafy vegetables ( Table 2) involved in this study, it was observed that total numbers of the detected compounds were found in cold pepper followed by cucumber, hot pepper, potato, okra, bean, carrot, squash, egg plant, cultivation tomato, green house tomato, onions, and cauli�ower. e total number of the detected compounds in such leafy vegetables in this study was 10,9,8,8,8,8,8,7,7,5,4,3, and 1 compound, respectively. e data tabulated in Table 2 also showed the detected amounts of pesticide residues in non leafy vegetables could be ranked in descending order as follows: cold pepper, egg plant, carrot, cucumber, potato, hot pepper, cultivation tomato, squash, bean, okra, onions, cauli�ower, and green house tomato which represent the lower non leafy vegetable contained pesticide residues. In terms of �gures, the sum of the detected pesticide residues in such non leafy vegetables were 1.507, 0.457, 0.302, 0.296, 0.293, 0.264, 0.239, 0.186, 0.163, 0.089, 0.0654, 0.062, and 0.05 ppm, respectively. From such ranking, it was observed that cold pepper, egg plant, and carrot were the most contaminated non leafy vegetables.
e frequency of the detected pesticide residues was calculated in the analyzed non leafy vegetables, it was found that the most frequent compounds was permethrin followed by deltamethrin and linden, endosulfan, chlordane, linuron, dimethoate, phosmet, and carbaryl. In terms of �gures, the frequencies for these pesticides were 11,8,6,5,5,5,4, and 4, respectively. e other detected compounds were frequented from three to one time. From the presented results it could conclude that cold pepper, egg plant, and carrot were the most contaminated non leafy vegetables, and the pyrethroids are the most frequented pesticides during the year of 2007.

Pesticide Residues in Leafy Vegetables during 2008.
In the agriculture season of 2008, data represented the detected amounts of pesticide residues in non leafy vegetables collected during the season of 2008 are listed in Table 3. According to the detected amounts and/or compounds, it was observed that the pattern of the detected pesticide residues in the collected non leafy vegetable samples were slightly differed from those presented in 2007 season. For example, in 2008 season, it was found the chloroneb, lindane, endosulfan, dicloran, and P,P-DDD as organochlorine compound, diazinon, phosphamidon, sulfotep, dimethoate, and mevinphos as organophosphorus, cypermethrin, deltamethrin, cy�uthrin, permethrin, resmethrin, tetramethrin, lambda cyhlothrin, and fenvalerate as pyrethroids. Also, it was detected propoxur, carbofuran, propamocarb, and bendiocarb as carbamates insecticides. As for fungicides, it found benomyl, tetradifon, and chlorobenzilate. As for the detected amounts, it is clear that cypermethrin represented the highest amounts of the detected residues which ranged between 0.086 and 0.762 ppm while the other detected concentrations were ranged between 0.077 and 0.0003 ppm. Overall, the pesticide residues which were found in this study were approximatly similar to other studies [12][13][14].
In addition, based on the number of detected residues, in non leafy samples, could be ranked in descending order as follow cucumber, cold pepper, potato, onions, okra, green house tomato, egg plant, bean, squash, hot pepper, cultivation tomato, cauli�ower, and carrot. e detected number of compound residues was 12.0, 9.0, 9.0, 8.0, 7.0, 7.0, 7.0, 6.0, 6.0, 5.0, 4.0, 4.0, and 3.0, respectively. As for the detected amounts of the mentioned pesticides, the ranking of non leafy samples becomes different to be egg plant is the most contaminated leafy samples followed by green house tomato < cold pepper < okra < cucumber < onions < cauli�ower < carrot < potato < bean < squash < hot pepper < cultivation tomato. In terms of �gures, the total detected amounts are 1.041, 0.849, 0.833, 0.694, 0.485, 0.344, 0.308, 0.298, 0.298, 0.216, 0.193, 0.183, and 0.057 ppm, for the mentioned leafy samples, respectively. In case of the frequencies of pesticide residues between the collected vegetables samples, it was observed that cypermethrin was the most frequented compound followed by endosulfan followed by dicloran and chlorobenzilate in which their frequencies were 7.0, 6.0, 5.0, and 5.0, respectively. e other detected compounds were frequented from four to one time. However, the presented data of 2008 season clearly shows that egg plant samples were the most contaminated non leafy vegetables followed by cold pepper, okra, and cucumber while hot pepper and cultivation tomato were the lowest contaminated vegetable samples. Data was mentioned previously partially in agreement with [15,16].   Table 4. From such data, it is clear that the majority of the analyzed vegetable samples collected in 2007 contained exhibited higher values than those of 2008 except �ve non leafy vegetables, that is, cold pepper, onions, beans, egg plant, and cauli�ower which were higher in their values than those of 2007. In addition, when the analyzed samples distributed between the four mainly seasons of each year, that is, winter, spring, summer, and autumn (based on the analyzed date), neither correlation nor trend could be observed between the pesticide residues content and the mentioned season. e selected plant foods will not give a for adverse biological effects to take place providing the residues of pesticides are controlled to be kept to a minimum. Pesticides residue monitoring programs should then be implemented to assure the minimum allowable residue levels in plant foods, especially with regards to permethrin, endosulfan, and deltamethrin [17].
e results of the detected amounts of pesticide residues in the selected vegetable, it is therefore clear that patterns of pesticide use are crop dependent: the predominant use of pesticides in vegetables is mainly to control a wide range of pests. In addition, the obtained results clearly indicate the actual situation of the misuse of insecticides which may affect in turn at long period the consumers health, and the most reasonable explanation for the highly detected pesticide residues in cold pepper, egg plant, and carrot may be due to the intensive use of insecticides and the highly deposited amounte of the applied compounds on the broad leafs of such vegetables. Overall, insecticides found in this study were similar to those found in other studies [12,13,[18][19][20][21].

Conclusion
However, the detected amounts of the mentioned insecticides in these important non leafy vegetables make the necessary to continue the pesticide residue monitoring programs which must be implemented to assure the minimum allowable residue levels in plant foods. With the LLC and GCMS multiresidue method, the optimum conditions were met to extract and determined 86 pesticides in more than 1057 vegetable samples less time and low detection limit (0.001 ppm). e samples no residues and contained pesticide residues at or below MRL were detected in 83.90%. Meanwhile, the detected pesticides concentration had exceeded the MRL in 15.89% of the total tested samples.