Instrumental and Sensory Analysis of the Properties of Traditional Chinese Fried Fritters

The quality of traditional Chinese fried fritters is typically measured using human sensory evaluation techniques and physicochemical indices, the process of which is laborious and time-consuming. This study aimed to investigate the relationship between instrumental parameters, sensory criteria, and physicochemical indices. Significant correlationswere foundusing principle component analysis. Volume, fat, texture, palatability, and instrumental parameters (hardness, fracturability, springiness, and gumminess) were found to be the main factors influencing the quality of Chinese fried fritters by principal component analysis (PCA) and instrumental methods, which were satisfactory replacement for human evaluation in correlation testing.


Introduction
Fried fritters are a type of traditional Chinese breakfast food and a popular snack, due to a low price and a distinctive flavor [1].The unique taste comes from the crisp surface, loose texture, and fried flavor.China produces approximately 120 thousand tons of fritters per year, worth 2.4 billion yuan.The income of the "Yong he King" chain from the sale of fried fritters in 2003 was reported to be 300 million yuan.Classified as a traditional Chinese food, fritters are not only eaten by the Chinese but also widely consumed abroad in countries including Japan, Korea, Singapore, and Russia.However, concerns over food quality standards have become a matter of debate all over the world and have gained significant attention in China.
Since 1990, many scientific studies have focused on fried fritters.Most have centered on the development of a new formula to enhance the quality and safety [2][3][4].Others have investigated the correlation between wheat flour and the quality of the final product [5,6].However, the evaluation criteria have been based only on human sensory evaluation and physicochemical indices.Sensory evaluation is commonly considered to be the best qualitative method of assessment and no instrumental techniques are thought to exist to adequately replace human evaluation of food quality.However, a range of shortcomings of the human evaluation method hamper its usage, such as inconsistent evaluation standards and the relatively laborious and time-consuming process involved [7].
A significant correlation between instrument-based and subjective sensory methods has been difficult to establish and a reliable relationship is not always feasible, according to the research of Harker et al. [8].Empirical and imitative instrumental tests have correlated with sensory texture descriptors with varying degrees of success, for fruits [7,[9][10][11], fried food [12,13], bread [14], and meat products [15].Texture profile analysis (TPA) has been used for many years as a substitute for human teeth to detect properties including hardness, fracturability, adhesiveness, springiness, cohesiveness, gumminess, chewiness, and resilience [16].
In the present study, 25 fried fritters made using different formulas were measured using both texture analysis and sensory evaluation.In order to determine the most effective instrumental parameter for the prediction of fried fritter texture, correlations between sensory perception and physicochemical and texture properties of fried fritters were investigated.

Ingredients.
Fried fritters were produced using 25 different formulas.The ingredients added for each formula were identical, and the differences between formulas were due to the amounts of the ingredients added.Flour was obtained from Tianjin (China).The leavening agent used included sodium bicarbonate (SB), gluconic acid--lactone (GdL), calcium biphosphate (CB), glycerol monolaurate (GML), yeast, and amylomaize starch (AMS).

Physicochemical Properties.
The moisture content of the fried fritters was determined by vacuum drying.Samples were cut into pieces (approximately 100 g) and dried in a vacuum oven at 90 ∘ C until the weight reached a constant value (modified method 44-40 [AACC, 2000]).Lipid content was measured gravimetrically following extraction using an extraction unit (SOXTEC System HT2, 1045) with petroleum ether (boiling range 30-60 ∘ C) [13].The volumes of the samples were determined using the rapeseed displacement method [17] and five samples of each formula were selected for volume testing.Specific volume was calculated from the ratio of volume to weight.All physicochemical measurements were carried out in triplicate.

Sensory Evaluation.
Nine individuals participated in the sensory evaluation test, following completion of a series of training sessions over a one-month period.Training consisted of identifying and rating the color, odor, palatability, and texture of the fried fritter samples.The criteria for each index in the sensory evaluation are listed in Table 1.

Instrumental Measurements.
TPA instrumental measurements were made using AXT-2i Texture Analyzer (Stable Micro System Ltd., Surrey, UK).Seven samples were selected from each formula for TPA testing.During the testing process, samples were kept separate from any factors that could potentially influence the results, such as samples temperature and air flow.Results were averaged following removal of values outside of two standard deviations.Specific TPA parameters were as follows: probe DPH-3PB, sensitivity 10 g, test speed 5.0 mm per min, speed before test 5.0 mm per min, speed after test 5.0 mm per min, test distance 70% (sample thickness), automatic trigger, and access rate 200 PPS.In instrumental measurement, many indices were obtained including hardness, fracturability, adhesiveness, springiness, cohesiveness, gumminess, chewiness, and resilience.

Statistical Analysis.
Principal component analysis and correlation testing were carried out using the SPSS 17.0 software package (SPSS, Chicago, IL, USA).All instrumental and sensory data were subject to Duncan's Multiple Range Test, with the level for statistical significance set at  < 0.05.Correlation coefficients were obtained using Pearson's Bivariate Correlation.Principle component analysis (PCA) was conducted to summarize the relationships between physicochemical properties and the sensory parameters.Large and uniform pore 8∼10 Large pore but general uniformity 4∼8 Small pore and poor uniformity 1∼4

Results and Discussion
All results pertaining to the quality of the fried fritters are listed in Tables 2, 3, and 4. In Table 2, moisture content of all samples ranged from 27.27% to 39.67%, fat content from 5.58% to 14.87%, and volume from 2.22 to 3.24.In Table 3, sample 8 had the best odor, sample 24 had the best color and texture, and sample 25 had the best palatability.In Table 4, sample 6 was best at hardness, gumminess, and chewiness and sample 3 had the best fracturability.

Correlation between Sensory Parameters, Physicochemical
Indices, and Texture.The correlations found between sensory properties, the physicochemical indices, and TPA-derived textural measurements are displayed in Tables 5 and 6.The correlations between fat content and palatability, volume, and texture were positive and significant ( < 0.01).Fat content and texture, volume, and palatability were significantly and negatively correlated ( < 0.05; Table 4).This indicates that fat content and volume have direct impacts on the quality of the final product and, therefore, that the quality of fried fritters could be regulated by controlling these properties.
Fracturability and springiness were found to be significantly correlated with fat content ( < 0.05).The most significant correlation for fracturability, however, was with volume ( < 0.01).It is proposed that oil could hinder moisture absorption of the product coating as it cools, resulting in crispiness.Additionally, springiness and chewiness were found to be significantly correlated with volume.Results as mean ± SD.Different superscripts in the same row denote significant differences ( < 0.05).
Textural properties also showed good correlation with sensory parameters, as shown in Table 6.A significant correlation was found between texture and fracturability, gumminess, and chewiness ( < 0.05).Hardness, fracturability, and chewiness were closely associated with palatability ( < 0.05).This indicates that the TPA technique could feasibly take the place of human sensory evaluation for fried fritters.

Principal Component Analysis. Fifteen indices including sensory evaluation and physicochemical and textural
properties were analyzed by PCA.Four main components were selected according to their eigenvalue.Eigenvalues and cumulative percentages are listed in Table 7.The loads of each index on the main components are listed in Table 8.
The variables relating to the quality of fried fritters were significantly explained by the indices represented by PC1, since PC1 explained most of the total variation (Figure 1).The correlation between the indices distributed on different axes was found to be poor.The correlation between every two indices distributed on two ends of one axis was found to be negative.PC1 was mainly influenced by chewiness, fracturability, palatability, hardness, springiness, gumminess, moisture, volume, and texture (Table 8, Figure 1).Volume and texture were found to be negatively correlated with chewiness, fracturability, hardness, palatability, moisture, springiness, and gumminess.Volume and texture were along the negative axis, while chewiness, fracturability, hardness, palatability, moisture, springiness, and gumminess were along the positive axis (Figure 1).The main factors for PC2 were resilience, odor, and fat content.However, resilience and odor were found to be negatively correlated with fat content.PC3 was mainly influenced by color and cohesiveness (Table 8, Figure 2) and they were distributed on the positive and negative dimension of axis, respectively.Moisture, palatability, and gumminess were the main variables explained by PC4.Moisture and palatability were loaded on the positive dimension while gumminess was on the negative dimension.
From Table 9 and Figure 3, the nature differences of samples are determined by the relative distance from the loading plots.The results for samples 3, 4, and 6 indicate that they rated highly for chewiness, frangibility, hardness, palatability, moisture, springiness, and gumminess, and samples 13, 11, 15, 21, 17, and 24 rated highly for volume and texture (Table 9, Figure 3).The results for sample 9 indicate that it rated highly for resilience and odor.Samples 6 and 4 not only rated highly for texture properties but also had high fat content (Figure 3).
As shown in Table 9 and Figure 4, the color of samples 8, 25, and 24 was much better than the others.Samples 5, 14, 13, and 7 were found to have the best cohesiveness.Sample 7 had not only high cohesiveness but also the highest water content.Samples 23 and 24 also had high moisture and a pleasing color.Samples 6 and 21 rated highly for gumminess.Results as mean ± SD.Different superscripts in the same row denote significant differences ( < 0.05).Different superscripts in the same row denote significant differences ( < 0.05).

Conclusions
The instrumental data demonstrated high correlations between physicochemical properties and sensory and textural properties of the Chinese traditional fired fritters.
Meanwhile, principle component analysis revealed that volume, fat, texture, palatability, hardness, fracturability, springiness, and gumminess were the main factors to influence their inherent qualities.To date, a novel way is needed to improve the quality of evaluation system, and  thus the method which combines instrumental data with principle component analysis could be an innovational and rapid assessment strategy for them.

8 Figure 1 :
Figure 1: The loading plot of term of fried fritters in 1 and 2 principal components (PC1 and PC2).

6 Figure 2 :
Figure 2: The loading plot of term of fried fritters in 3 and 4 principal components (PC3 and PC4).

Figure 3 :
Figure 3: The score plot of samples in 1 and 2 principal components (PC1 and PC2).

Table 1 :
The sensory evaluation criteria of fried fritters.

Table 2 :
Physicochemical results of all the fried fritters samples.

Table 3 :
Sensory results of all the fried fritters samples.

Table 4 :
Texture properties of all the fried fritters samples.

Table 6 :
The correlation between sensory evaluation and TPA results.

Table 7 :
Eigenvalues and cumulative percentage of first five principal components.

Table 8 :
Eigenvalues for principal components for all the parameters of fried fritters.