Phenolic Compounds, Antioxidant Activity, and Other Characteristics of Extra Virgin Olive Oils from Italian Autochthonous Varieties Tonda di Villacidro, Tonda di Cagliari, Semidana, and Bosana

1Department of Life and Environmental Sciences, University of Cagliari, Via Ospedale 72, 09124 Cagliari, Italy 2Department of Organic Chemistry, Faculty of Chemistry and Technology, University of Split, N. Tesle 10/V, 21000 Split, Croatia 3Department of Chemistry and Pharmacy, University of Sassari, Via F. Muroni 23/b, 07100 Sassari, Italy 4Department of Biomedical Sciences, Unit of Experimental Pathology, University of Cagliari, Cittadella Universitaria SS 554, Monserrato, 09042 Cagliari, Italy


Introduction
Extra virgin olive oil (EVOO) is a worldwide recognised high valuable food product and there are constant efforts to enhance its quality and to preserve it from adulteration.In the last decades, several analyses to identify different olive oil cultivars and to verify the presence of any adulteration have been developed [1,2].The quality of olive oil is directly connected with the variety of the olives and there is a strong link between the cultivar and the territory of cultivation [3,4].Extra virgin olive oil from Sardinia (Italy) is protected by the European Union "Protected Designation of Origin" (PDO "Sardegna") and it can be obtained from several autochthonous olives as Tonda di Villacidro, Tonda di Cagliari (also known as Nera di Gonnos), Semidana, and Bosana [5].More than 20 cultivars were defined so far in Sardinia and wild olive forms are still widely represented [6].Archaeological evidence proves that olive oil extraction was performed in ancient times, long before different olive cultivars were introduced by Phoenicians, Romans, and Spanish peoples [6].The PDO "Sardegna" was created mainly to promote the sensory properties, but also to preserve the health benefits of the most typical olive oils of the region.The EVOOs obtained from Tonda di Villacidro, Tonda di Cagliari, Semidana, and Bosana have specific sensory characteristics and a long tradition in Sardinia [6][7][8][9].Good yield and quality were observed in Semidana and Bosana for oil production, while Tonda di Cagliari resulted as interesting dual-purpose cultivars [7].Some data on the physical-chemical parameters of these monovarietal oils can be found in scientific databases.The Bosana cultivar has been shown to have a high content of phenolic compounds [10].Nevertheless, data in the main Sardinian EVOO are fragmentary and, to the best of our knowledge, no data on Tonda di Villacidro has been published so far.
The aim of this paper is to perform a chemical characterization of Tonda di Villacidro, Tonda di Cagliari, Semidana, and Bosana EVOOs by (1) determination of basic technological characteristics (acidity, peroxide value, K 232 , and K 270 ) according to EU regulations; (2) the fatty acids (GC-FID/MS) and triacylglycerols profiles (HPLC-DAD); (3) total phenols, chlorophylls, and carotenoids evaluation by UV/vis including targeted tocopherols analysis by HPLC-FL; (4) HPLC-DAD targeted phenolics analysis of the hydrophilic oil fractions (HFs) and assessing of the HFs antioxidant activity (with DPPH • , ABTS •+ , and FRAP assays).

Olive Oil Samples and Hydrophilic Fraction Extraction.
Extra virgin olive oils ( = 61) from Tonda di Villacidro ( = 15), Tonda di Cagliari ( = 14), Semidana ( = 14), and Bosana ( = 18) cultivar were obtained from olive fruits by local groves in Sardinia (Italy) in the years 2013-2014.Four batches of healthy olive fruits (each ca.200 kg) were harvested in the same ripening index (RI = 4.3 ± 0.2) [11], processed within 24 h to olive oils, and stored in dark glass bottles at 12 ± 1 ∘ C until analysis.Sensory analysis of the samples was performed under the conditions described within the EC Regulation 640/2008 [12] and the samples were analyzed within 3 months from their production.Hydrophilic fractions (HFs) of the oils were prepared as reported by Tuberoso et al. [13].

Fatty Acids and Squalene. A transmethylation technique
followed by GC-FID/MS determination was used [13,15].The percentage composition of the oils was calculated from GC peak areas without using correction factors.The quantitative analysis of squalene was performed using the internal standard method (with squalane) and results were expressed as mg squalene/kg of sample.
2.5.Triacylglycerols.The analysis of triacylglycerols (TAG) was performed with an HPLC-UV method [13].Calibration graphs were constructed with the external standard method by measuring peak area versus concentration ( = 0.9982-0.9997).The concentrations of the compounds were calculated in mg/kg and data were expressed in weight percentages.Standard solutions of LLL, LLO, LLP, OOL, POL, OOO, OOP, PPO, and OOS were prepared in acetone.The use of equivalent carbon number (ECN = CN − 2, where CN is the number of carbon atoms and  is the number of double bonds) allowed the attribution of compounds of which no analytical standards were found.In this way LnLL, LnLno, LnLnLn, LLnLn, OLLn, PLLn, OOLn, POLn, LPP, PSL, and SSLn were identified and the quantification was performed using the calibration curves of the TAG standard with the closest chemical structure and ECN number.

Targeted Phenolic Compounds
Analysis.Detection and quantitative analyses of HF phenolic compounds were carried out using a LC-DAD method as described by Šarolić et al. [15].Calibration curves were constructed with the external standard method, correlating the area of the peaks with the concentration.All compounds were dosed using the calibration curve built with the respective standard, except oleuropein and ligstroside derivatives that were dosed using oleuropein calibration curve.The correlation values were comprised between 0.9993 and 0.9998.Oleuropein and ligstroside derivatives and acetoxypinoresinol were tentatively identified by comparison with literature data [16][17][18].

Determination of Tocopherols, Total Chlorophylls, and
Carotenoids.An HPLC system connected to a spectrofluorometer detector was used to dose and -tocopherols [15].Standard solutions were prepared in acetone, while working solutions were prepared to appropriate dilution with the eluent mobile phase.Linearity in the range 0.1-6 mg/kg was 0.9998.Total chlorophylls and carotenoids were estimated spectrophotometrically reading the absorbances at two different wavelengths (464 nm for carotenoids and 669 nm for chlorophylls) [14].Chlorophyll a and -carotene stock standard solutions were prepared in acetone, as well as working solutions, which were prepared with proper dilutions (0.1-2.0 mg/kg,  = 0.9996, and 0.02-0.50mg/kg,  = 0.9995, for chlorophyll a and -carotene, resp.).

Determination of Total Phenolic Content (Folin-Ciocalteu's Assay).
Total phenolic content of the HF was estimated spectrophotometrically with modified Folin-Ciocalteu's method [19].The total polyphenol content results, expressed as mg/kg of gallic acid equivalent (GAE), were obtained using a calibration curve of a freshly prepared gallic acid standard solution (5-100 mg/kg,  = 0.9998).

Free Radical Scavenging Activity (DPPH • and ABTS •+
Assays) and Total Antioxidant Activity (FRAP Assay).The in vitro HF antiradical activity was assessed with the DPPH • spectrophotometric method and the obtained data were expressed as Trolox equivalent antioxidant capacity (TEAC) [15].A Trolox calibration curve in the range 0.02-1.00mM was prepared ( = 0.9998), and data were expressed in Trolox equivalent antioxidant capacity (TEAC, mmol/kg).The ABTS assay was performed according to Re et al. [20] with slight modification [19], and data were expressed as TEAC values.
The FRAP assay was performed preparing a ferric complex TPTZ and Fe 3+ [19][20][21].Quantitative analysis was performed according to the external standard method (FeSO 4 , 0.1-2.0mmol/kg,  = 0.9999), correlating the absorbance with the concentration, and results were expressed as mmol/kg of Fe 2+ . .These values meet also the requirement of the PDO "Sardegna" production specification [5].The analysis of fatty acids and triacylglycerols allowed finding in EVOO 13 fatty acids and 16 different triacylglycerols.Table 2 shows the fatty acid composition of Tonda di Villacidro, Tonda di Cagliari, Semidana, and Bosana EVOO.No significant differences were observed between these oils concerning the amount of each quantified fatty acid, and among these oleic, palmitic, and linoleic acid were the most concentrated, consistent with other previously published data   [24,25].Bosana oil showed a slightly higher amount of oleic acid (66.4 ± 3.0%): this is reflected in the analysis of triacylglycerols content (Table 3), which consists mostly in OOO, POO, and LOO, in all the EVOO samples, but in particular in that of Bosana variety.Antioxidant compounds are fundamental for both oil stability and nutritional aspects.Lipophilic antioxidant compounds (-and -tocopherols, squalene, chlorophylls, and carotenoids) were quantified by different analytical methods and the results are reported in Table 4. Squalene, which is an important molecule that gives oils some pharmacological properties [26], dominates in all samples (about 5000 mg/kg), followed by tocopherols, carotenoids, and chlorophylls.Tonda di Villacidro and Semidana EVOO exhibited the highest amount of squalene (6232.8± 1330.8 and 6023.2 ± 1296.4 mg/kg, resp.), whereas Bosana EVOO showed the highest amount of tocopherols (213.3 ± 55.4 and 8.7 ± 4.7 mg/kg for and -tocopherol, resp.).As regards these compounds, the varieties included in the EVOO PDO "Sardegna" production specification must have an amount in tocopherols ( + ) at least of 100 mg/kg [5] and this value is exceeded in all the analyzed samples (Table 4).
The hydrophilic extracts obtained from four monovarietal EVOOs were also analyzed by LC-DAD to quantify the main phenolic compounds and the results are shown in Table 6.Significant differences among most of the phenolic  constituent levels were observed from the extracts.Taking into account that the mechanical extraction process (grinding, malaxing, and separation) was very similar for both oils, differences in phenolic compounds amount can be primarily due to the variety but can be affected also by year, peculiar atmospheric conditions, agronomic practices, storage, and maturation grade [30][31][32].Targeted analysis showed that the most abundant secoiridoids of the samples were dialdehydic form of elenolic acid linked to hydroxytyrosol or tyrosol (p-HPEA), respectively, assigned as 3,4-DHPEA-EDA, 3,4-HPEA-EA, and p-HPEA-EDA.Other phenolics such as vanillic acid, p-coumaric acid, flavones (luteolin and apigenin), and lignans (pinoresinol and 1-acetoxypinoresinol) were found in almost all analyzed samples.Bosana HF showed the highest amount of the targeted phenolic compounds dosed (142.2 ± 4.7 mg/kg), confirming that the oil obtained from this cultivar is one of the richest in polyphenols [10].This extracts were characterized statistically by the highest amount of 3,4-HPEA-EA and 3,4-DHPEA-EDA (84.7 ± 55.4 and 35.8 ± 19.9 mg/kg, resp.).This HF was also the richest in hydroxytyrosol and tyrosol, phenolic compounds highlighted for their health benefits [33,34].

Conclusions
In this work were highlighted the chemical characteristics of the four EVOOs that are included in PDO "Sardegna."They exhibited all parameters within the limits for the EVOO category; moreover, all the EVOOs showed levels of tocopherols and phenolic compounds higher than the minimum reported in the PDO "Sardegna" production specification, therefore satisfying the parameters of the Protected Designation of Origin.Inside each variety, some peculiarities in the analytical data can be noticed, but the strong variability makes them not significant.The analysis of fatty acids and triacylglycerols composition does not help to clearly differentiate the four Sardinian varieties.The phenolic profile of the EVOO's HF may be more useful.Bosana HFs confirm data that were previously reported in the literature, proving to be the oil rich in antioxidants (lipophilic and hydrophilic) and then with a huge antioxidant activity.Anyway, all the HFs from four varieties showed a good antioxidant capacity and it may be interesting to appreciate the product not only from the sensorial point of view, but also from that of nutraceutical use.

Table 1 :
Technological characteristics of Tonda di Villacidro, Tonda di Cagliari, Semidana, and Bosana EVOO.Min Max Mean ±SD Min Max Mean ±SD Min Max Mean ±SD Min Max Mean ±SD Threshold value for EVOO is ≤0.8; b threshold value for EVOO is ≤20; c threshold value for EVOO is ≤2.5; d threshold value for EVOO is ≤0.22 [EEC Regulation 2568/91]. a

Table 5 :
Total phenolic content and antioxidant activities of hydrophilic fraction of Tonda di Villacidro, Tonda di Cagliari, Semidana, and Bosana EVOO.±SD Min Max Mean ±SD Min Max Mean ±SD Min Max Mean ±SD GAE: gallic acid equivalent.b DPPH • and ABTS •+ values are expressed as TEAC millimolar concentration, obtained from a Trolox solution having an antiradical capacity equivalent to that of the dilution of the EVOO.c FRAP value is expressed as Fe 2+ millimolar concentration, obtained from a FeSO 4 solution having an antioxidant capacity equivalent to that of the dilution of the EVOO. a