Nafion „-Containing Solid Lipid Nanoparticles as a Tool for Anticancer Pt Delivery : Preliminary Studies

Preliminary studies of nanoparticles based on the perfluorosulfonic acid resin Nafion have been carried out with the aim of establishing an ionic connection with the protonable phosphine 1,3,5-triaza-7-phosphaadamantane (PTA), suitable for the coordination of platinum. Nafion-containing nanoparticles (NAF) were produced by homogenization followed by ultrasonication method. After production, NAF were characterized in terms of size, morphology, and in vitro cytotoxicity.The PCS studies showed that theZ-averagemean diameter was around 250 nm.Moreover, the polydispersity index showed amonodimensional distribution of nanoparticles. Cryo-TEM analysis showed a uniform and homogenous population of particles, characterized by the presence of both ovoidal and needle-like structures. To evaluate the in vitro cytotoxicity, NAF were tested on human cancer cell lines K562 and A2780. No cytotoxic effect was found on both cell lines. By P-NMR measures, it is here proved that the strongly acidic sulfonic groups of Nafion-containing nanoparticles (NAF) can act as protonating agents for PTA. The protonation occurs selectively at nitrogen; hence protonated PTA maintains its ability to coordinate platinum via its phosphorus atom.


Introduction
The amino Pt complexes like cisplatin and analogues are widely used in medical oncology for management of tumors of the ovary, testes, head, and neck and other cancers [1,2].An extensive search for platinum-based drugs with improved pharmacological properties and a wider spectrum of action as compared to cisplatin has been developed.It has been supposed that the distribution and the concentration of platinum-based drugs in different body districts are influenced by physicochemical properties of the ligands such as polarity, hydrophilicity, and steric requirements [3].Among several other modifications, the possibility of structurally modifying cisplatin by introducing phosphines as neutral ligands has been considered [4,5].
The search of a strategy aimed to the extension of Pt pharmaceutical application to phosphinic complexes has attracted great interest since many years.To this purpose nanoparticulate drug delivery systems have been considered since they can improve the permeability, safety, pharmacokinetics properties, and bioavailability of the therapeutic substances in the treatment of tumor cells [6].
The phosphine 1,3,5-triaza-7-phosphaadamantane (PTA) (see Table 1) [7] looks particularly suitable for pharmaceutical application because it possesses reactivity and size comparable to that of alkylphosphines [8] with the addition of valuable properties like hydrosolubility, stability to oxidation, and lack of toxicity.
PTA can be selectively alkylated or protonated on nitrogen, thus maintaining its coordinative ability, which is achieved by phosphorus.N-alkylated PTA derivatives can also be introduced as ligands in platinum or ruthenium complexes designed for anticancer activity [9,10].We recently proposed a delivery system for Pt-PTA complexes based on the use of solid lipid nanoparticles (SLN) of stearic acid that should favour both the delivery and the activity of the Pt-PTA moiety [11].
As an alternative route for including Pt-PTA complexes in solid lipid nanoparticles, in this paper we considered the formation of an electrostatic interaction between appropriate proton donating functionalities and the proton acceptor nitrogen of PTA.To this purpose the fluoropolymercopolymer Nafion (Table 1) was evaluated.
Nafion is a perfluorinated polymer that contains small proportions of sulfonic functional groups.Although the exact structure of Nafion is not known, its structure can be resembled to that of an ionic surfactant in which the hydrophilic head is typified by the sulfonic functional groups and the hydrophobic portion by the tetrafluoroethylene structure.In this view, the use of Nafion in the composition of a lipid based nanoparticles could give rise to the formation of a structure in which the lipophilic portion of Nafion lies inside the lipid core of the particles, while its polar moiety is present on particle's surface.The presence of the sulfonic groups on the surface could thus be used to interact with Pt-coordinated PTA.
Particularly, the present study reports (i) the preparation of Nafion-containing nanoparticles (NAF), (ii) their characterization in terms of size, morphology, and X-ray diffraction, (iii) the test of their cytotoxic activity in vitro on two different human cancer cell lines, namely, erythroleukemic K562 and ovarian cancer A2780 cells, and (iv) the 31 P NMR investigation of the ability of NAF to selectively protonate PTA on nitrogen and of the coordination ability of the NAF/PTAH + system to Pt.
2.2. 31 P NMR Analyses.NMR spectra were recorded using a Varian Gemini 300 MHz spectrometer ( 31 P at 121.50 MHz).The 31 P spectra were run with proton decoupling and 31 P signals are reported in ppm relative to an external 85% H 3 PO 4 standard.No deuterated solvent is required.An internal capillary containing C6D6 placed inside the tube provides the deuterium necessary for the instrument locking. 31P NMR data are reported in Table 1.
The concentration of -SO 3 H groups in NAF was estimated 9.0⋅10 −4 mol/L.An equimolar amount of PTA was added and the 31 P NMR spectrum was acquired (18 h acquiring time).
Half equivalent of platinum was then added (4.5⋅10 −4 mol/L as K 2 PtCl 4 ) and the 31 P NMR spectrum was acquired (60 h acquiring time).

NAF Preparation.
NAF were prepared by homogenization stirring followed by ultrasonication.Briefly a mixture composed of 3.35% w/w of tristearin and 1.65% w/w of Nafion was melted at 80 ∘ C. The mixture concentration was 5% w/w with respect to the total weight of dispersions.To the fused lipid phase 4.75 ml of an aqueous poloxamer 188 solution (2.5% w/w) was added at 80 ∘ C under 15,000 rpm high-speed stirrer for 1 min, using a Ultra Turrax T25 (IKA-Werke GmbH & Co. KG, Staufen, Germany).The emulsion was then subjected to ultrasonication (MicrosonTM, Ultrasonic cell Disruptor) at 7 kHz for 15 min and cooled down to room temperature by placing it in a water bath at 22 ∘ C. NAF dispersions were stored at room temperature.

Photon Correlation Spectroscopy (PCS).
Submicron particle size of different batches of nanoparticles was determinate using a Zetasizer Nano S90 equipped with a 4 mW helium neon laser with a wavelength output of 633 nm.Glassware was cleaned of dust by washing with detergent and rinsing twice with water for injections.Measurements were made at 25 ∘ C at an angle of 90 ∘ C. Samples were diluted 1 : 10 (v/v) with the aqueous phase of each formulation.Each experimental value results from three independent experiments performed in triplicate.Data were interpreted using the "method of cumulants" [12].

Cryotransmission Electron Microscopy (Cryo-TEM).
For cryo-TEM studies, a sample droplet of 2 l was put on a lacey carbon filmed copper grid (Science Services, Munich), which was hydrophilized by air plasma glow discharge (Solarus 950, Gatan, Munich, Germany) for 30 s. Subsequently, most of the liquid was removed with blotting paper leaving a thin film stretched over the lace holes.The specimens were instantly shock frozen by rapid immersion into liquid ethane cooled to approximately 90 K by liquid nitrogen in a temperaturecontrolled freezing unit (LEICA EM GP, Germany).The temperature was monitored and kept constant in the chamber during all the sample preparation steps.The specimen was inserted into a cryotransfer holder (CT3500, Gatan, Munich, Germany) and transferred to a Zeiss/LEO EM922Omega EFTEM (Zeiss Microscopy GmbH, Jena, Germany).Examinations were carried out at temperatures around 90 K.The TEM was operated at an acceleration voltage of 200 kV.Zero-loss filtered images (DE = 0 eV) were taken under reduced dose conditions (100-1000 e/nm 2 ).All images were registered digitally by a bottom mounted CCD camera system (Ultrascan 1000, Gatan, Munich, Germany) combined and processed with a digital imaging processing system (Digital Micrograph GMS 1.9, Gatan, Munich, Germany).
2.6.X-Ray Diffraction.X-ray diffraction experiments were performed to characterize the inner structure of nanoparticles containing Nafion.A 3.5 kW Philips PW 1830 X-ray generator (Philips, Eindhoven, Netherlands) equipped with a homemade Guinier-type focusing camera operating in vacuum with a bent quartz crystal monochromator ( = 1.54 Å) was used.Diffraction patterns were recorded on a GNR Analytical Instruments imaging plate system (GNR Analytical Instruments Group, Novara, Italy).Samples were held in a tight vacuum cylindrical cell provided with thin Mylar windows.Diffraction data were collected at ambient temperature (37 ∘ C), using a Haake F3 thermostat (Thermo-Haake, Karlsruhe, Germany) with an accuracy of 0.1 ∘ C.
The position of Bragg peaks detected in the low-angle Xray diffraction region ( < 0.6 Å−1 , being  the modulus of the scattering vector defined by  = 4 sin /, where 2 is the scattering angle) was measured and peak indexing was performed considering the different symmetries commonly observed in lipid phases [13].

Growth Inhibition and Antiproliferative Activity Assays.
Cell growth inhibition assays were carried out using human cells, namely, erythroleukemic K562 and ovarian cancer A2780 cell lines.Cells were maintained in RPMI 1640, supplemented with 10% calf serum, penicillin (100 U/mL), streptomycin (100 g/mL), and glutamine (2 mM); the pH of the medium was 7.2 and the incubation was at 37 ∘ C in a 5% CO2 atmosphere.Cells were routinely passed every three days at 70% of confluence; for the adherent cell lines, 0.05% trypsin-EDTA was used.
The antiproliferative activity of compounds was tested with the MTT assay K562 or A2780 cells were seeded in triplicate in 96-well trays and NAF dispersions were diluted in culture medium to final concentrations ranging from 5 to 50 M.
Cells were exposed to the formulations for 72 h; then 25 l of a 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide solution (MTT) (12 mM) was added and incubated for 2 h.Afterwards 100 l of lysing buffer (50% DMF + 20% SDS, pH 4.7) was added to convert the MTT solution into a violet coloured formazane.After additional 24 h the solution absorbance, proportional to the number of live cells, was measured by spectrophotometer at 570 nm and converted into percentage of growth inhibition [9].

Results and Discussion
The formulation of Nafion-containing nanoparticles (NAF) was designed as a tool for loading PTA through the electrostatic interaction between Nafion sulfonic groups (strong proton donors) and the proton acceptor nitrogen atoms of PTA.
The obtained NAF/PTAH + system should maintain its ability to coordinate Pt via its P donor atom [14].
As a first point, the ability of Nafion to protonate PTA was checked in water solution by 31 P NMR.It was found that the signal of PTA in water (−100 ppm) is shifted at −90.7 ppm in the presence of Nafion, indicating the complete Nprotonation of the phosphine (Table 1).
As a second step it was then necessary to prove that the protonating ability of Nafion is maintained in the NAF formulation.
NAF were produced by homogenization followed by ultrasonication method as described in Materials and Methods.The formulation appeared as a milky white emulsion (see Figure 1), characterized by a uniform appearance with no aggregates and no adherence to the vial's walls.After production, NAF were characterized in terms of morphology and size by cryo-TEM, X-ray diffraction, and PCS analyses.
Cryo-TEM analysis was effectuated to shed light on the internal structures of particles dispersed in plain NAF and Figure 1 shows the resultant image.In general, NAF dispersions are mainly constituted of discoid-shaped structures.Particularly, when viewed from the top, the particles appear as circular or ellipsoidal, or as dark rods or "needles," depending on particle position and on their thickness.Moreover, some structures evidence the inner lamellar morphology of nanoparticles, probably due to the presence of tristearin mixed with Nafion (liquid at room temperature) resulting in the typical "sandwich-like" appearance.
Figure 2 shows the X-ray diffraction results for nanoparticles without and with NAF.Particularly, nanoparticles without NAF were used for comparison.It was found that X-ray Figure 2: X-ray diffraction profiles observed for nanoparticle dispersions without (black dots) and with NAF (white dots) at 37 ∘ C. The SLN profile has been shifted for clarity.Vertical lines indicate the position of the first 3 peaks expected in the case of a 1D lamellar symmetry: note that, in the NAF profile, the second order of diffraction is almost appreciable, confirming the lamellar symmetry.
profiles are very similar, with only one peak clearly resolved in the low-angle region.The corresponding unit cell, assuming a lamellar structure, is 49 and 46 Å for nanoparticles with and without NAF.Thus the presence of Nafion does not induce structural modifications in the internal structure of nanoparticles.
Size, polydispersity, and stability of NAF during time were evaluated by PCS analysis.The obtained results are showed in Table 2.The analysis was repeated over a period of three months and during this time the mean diameter was maintained between 230 and 260 nm indicating a dimension of particles according to pharmaceutical literature [15].Regarding the polydispersity, the indices were stable during time approximately indicating a great distribution of nanoparticles and a homogenous state, also confirmed by the macroscopic aspect (see Figure 1).On the whole, all the values were acceptable in terms of both size and polydispersity and the NAF were proven to be stable within ninety days.Indeed, this agreeable characterization could be again ascribed to the presence of Nafion in the liquid form.Generally, the addition of a liquid lipid in the formulation of nanoparticles allows a better shape of the particle matrix.Hence, the liquid form of lipid in NLC affords the homogeneous and uniform aspect of the final preparation [16].
Recently, nanoparticulate self-assemblies of lipid surfactants in aqueous media received much attention as safe drug delivery systems [17].Lipid nanocarriers can improve safety of product by solubilizing and stabilizing drug molecules, targeting them to the site of action and/or changing the pharmacokinetics of drugs since lipid based nanomedicines, after systemic administration, distribute differently in the body, and interact differently with biological components at cellular/subcellular level compared to formulations of free drug molecules [17].Concerning nanoparticles containing ionic interactions it has to be underlined that the toxicity of the system is a good starting point in the evaluation of toxicity and safety.Notably, lipids are physiological compounds characterized by high safety profile, most of them being components of food sources naturally present metabolic pathways for their degradation.Nafion may be a more notable issue in terms of toxicity evaluation since it is a synthetic product and do not find application in pharmaceutical products.In order to evaluate the in vitro cytotoxicity, NAF were tested on K562 and A2780 cell lines.Figure 3 graphically shows the percentages of cell growth inhibition generated by NAF on K562 and A2780 cell lines.
On K562 cells was evidenced by a dose-dependent profile up to a concentration of 25 M getting around 8%.On A2780 cells a dose-dependent profile was clearly underlined.The higher percentage of cell growth inhibition was of around 15% using a concentration of 50 M.
Considering the low values of cell growth inhibition, NAF showed no cytotoxic effect on both cell lines and these results suggest the possibility of studying the interaction with Pt-PTA complexes and the antiproliferative activity as a consequence.
The NAF suspension was characterized by a pH value of 3.31 ± 0.15 (similar to that of an equimolar aqueous solution of Nafion = 3.01 ± 0.03).It was mixed to a solution containing a PTA amount equimolar to the concentration of -SO 3 H groups.The resulting mixture was subjected to a long scanning 31 P NMR acquisition (18 h), required by the low phosphorus concentration.As demonstrated by the data reported in Table 1, it was found that, after the interaction with NAF, the signal of PTA shifts at −90.6 ppm; the same shift was observed in solution, thus demonstrating the protonating efficiency of nanoparticles containing Nafion and the formation of an ionic interaction between NAF and PTA.This result confirmed our hypothesis of the presence of Nafion sulfonic groups on the surface of the nanoparticles and their ability to form a strong interaction with a proton acceptor.
The ability of NAF/PTAH + to coordinate Pt through the phosphorus site of PTAH + was then checked.K 2 PtCl 4 , a water soluble source of Pt 2+ (half equivalent with respect to PTA, the expected product being NAF-supported cis-[Pt(PTAH + ) 2 Cl 2 ]), was added to the NAF/PTAH + suspension and 31 P NMR was acquired over 60 hours.
The obtained 31 P NMR spectrum showed a signal shift from −90.6 ppm to −49.5 ppm (typical of Pt-coordinated PTA in a cis-geometry) which unequivocally proved the complete loading of platinum and the formation of NAF-supported cis- The observation of Pt-satellites and the Pt-P coupling constant was not possible, because the observed signal was weak and broad, due to the low phosphorus concentration and the probable presence of rapid equilibria.
Concerning their morphology NAF-supported cis-[Pt(PTAH+)2Cl2] nanoparticles do not differ from NAF being characterized by ellipsoidal particles or dark rods (data not shown).In addition, as reported in Table 3, in terms of size PCS analysis demonstrated that NAF-supported cis-[Pt(PTAH+)2Cl2] nanoparticles are quite stable over a period of 1 month.

Conclusions
Considering the results obtained in this study, the following conclusions can be drawn.NAF were found stable during time, with a uniform appearance, a size of around 250 nm, and a polydispersity of around 0.2.The morphological aspect of these nanoparticles was characterized by both ovoidal and needle-like structures, constituting a homogeneous population.The acid pH value of NAF suggested the possibility of protonating PTA; this ability was studied demonstrating the formation of the complex NAF/PTAH + which then successfully coordinated Pt.The evaluation of the in vitro cell growth inhibition on K562 and A2780 cell lines demonstrated the lack of cytotoxicity of NAF and their promising use as vehicles for Pt-PTA complexes.
Further experiments are necessary to increase the final platinum concentration and to test the antiproliferative activity of Pt-containing NAF/PTAH + preparation.Nevertheless, this work represents the first example of pharmaceutically aimed use of Nafion and of the use of electrostatic interactions for nanoparticles functionalization.Moreover, further studies could be aimed at investigating the possibility, in NAF/PTAH + preparation, of switching the loading and detaching of platinum by pH or ionic strength modulation.

% of cell growth inhibition 5 Figure 3 :
Figure 3: In vitro antiproliferative effect of NAF on K562 cells and A2780 cells.Data represent the percentages of cell growth inhibition compared to untreated control cells.Data are the mean of three independent experiments ± s.d.conducted in triplicate. values are always <0.01.

Table 1 :
31P NMR data in D 2 O.

Table 2 :
-average diameter and polydispersity of NAF during time.