Review of Upflow Anaerobic Sludge Blanket Reactor Technology : Effect of Different Parameters and Developments for Domestic Wastewater Treatment

1 Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou, China 2Department of Biotechnology and Genetic Engineering, Kohat University of Science and Technology, Kohat 26000, Pakistan 3Department of Environmental Sciences and Engineering, Government College University, Allama Iqbal Road, Faisalabad 38000, Pakistan 4Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China 5Institute of Soil & Environmental Sciences, University of Agriculture, Faisalabad 38000, Pakistan


Introduction
The wastewater generated by a community is called "sewage," which is a mixture of domestic wastewater, industrial wastewater (where the industry is discharging its wastewater in the same sewage system), and rain water, where a single sewer system exists for wastewater and storm water [1].In the developing/underdeveloped countries of the world, more than 90% of the sewage is discharged untreated in the environment due to lack of proper wastewater collection and treatment facilities.The quantity and strength of wastewater are governed by the size and socioeconomic condition of the population of the area [2].The composition of sewage varies greatly and its characterization is important for determining the size and designing of treatment plant [3].Table 1 provides an overview of characteristics of municipal sewage in various cities of the world.
Anaerobic treatment is preferred to treat municipal wastewater because of its merits over conventional treatment methods [1].These advantages are (i) its ability to treat high COD loads and withstand fluctuation in the influent, (ii) biogas formation, and (iii) effective treatment of wastewater in a short period of time [4].Anaerobic reactors reduce pollution load and provide good stabilization of solids.Furthermore, depending on the design of a UASB reactor, a high sludge hold-up time can be obtained so that the excess sludge needs to be discharged only once every three to four years [5].The comparison of aerobic and anaerobic technologies is given in Table 2.

Parameters
Pakistan [82] Palestine Brazil Columbia Netherlands Karachi Lahore Al Bireh [3] Pedregal [1] Cali [1] Bennekom [ The major achievement in the development of anaerobic treatment was the introduction of high rate reactors in which biomass retention and liquid retention were not interlinked [5].Among the various anaerobic wastewater treatment technologies, upflow anaerobic sludge blanket (UASB) reactors have achieved considerable success and these reactors have been applied to treat a wide range of effluents such as sugar, pulp and paper, dairy, chemical, potato starch, bean balancing, soft drinks, fish processing, noodle processing, yeast production, slaughterhouse, and coffee processing industries [6][7][8].The UASB process was developed by Lettinga and coworkers in the late 1970s [6].It is primarily used for the treatment of highly concentrated industrial wastewaters [7][8][9]; however, it can also be used for the treatment of low strength wastewater such as municipal wastewater with relatively lower contaminant strength [10][11][12][13][14].As compared to aerobic technologies anaerobic treatment systems such as UASB are being encouraged because of several advantages, including plain design, uncomplicated construction and maintenance, small land requirement, low construction and operating cost, low excess sludge production, robustness in terms of COD removal efficiency, ability to cope with fluctuations in temperature, pH and influent concentration, quick biomass recovery after shutdown, and energy generation in the form of biogas or hydrogen [1,9,[15][16][17][18][19].These characteristics make UASB a popular wastewater treatment option [20,21] and a large number of researchers have recommended UASB technology for the treatment of sewage wastewater in tropical and subtropical regions [10-12, 14, 22-24].
It is particularly appealing in tropical countries where the comparatively high ambient temperature is nearly optimum for the mesophilic methanogenic bacteria [11,13].Significant efforts were made in the past twenty years to ascertain the mass transfer and kinetic processes going on in the reactor.In this study, the advantages and disadvantages of anaerobic and aerobic bioreactors are highlighted and comparison is made of UASB with other sewage treatment methods to state their feasibility and efficiency in domestic wastewater treatment and identify possible future areas of research.The effects of main process parameters, temperature, HRT, OLR, pH, granulation, and mixing on the performance of UASB reactors and hydrogen production are provided for optimal growth of bacteria and performance of the system.Appropriate posttreatment options are also identified to be potentially used in developing countries having appropriate climate conditions.

Upflow Anaerobic Sludge Blanket Reactor Process
Wastewater to be treated is introduced from the bottom of the reactor and it flows upward through a blanket of biologically activated sludge, which is generally in the form of granular aggregates.The sludge aggregates have very good stability and do not get washed out under practical conditions and therefore provide good treatment efficiency when the wastewater comes in contact with the granules [25].The gases (methane and carbon dioxide) produced under anaerobic conditions cause internal mixing, which helps in the formation and maintenance of biological granules.However, some of the gas produced in the sludge blanket is attached to the granules, and a gas-liquid-solid separator (GLSS) is added on the top of the reactor for the effective segregation of gas, liquid, and granules.In GLSS, the gas surrounded particles strike with the bottom of degassing baffles and fall back into the sludge blanket and the treated water flows out of the reactor [21,26,27].
There is lower gas production in sewage as compared to high strength wastewater, which leads to less circulation of gas to support the formation of biological granules.Therefore, control of channeling is important for weaker wastewaters like sewage [6,13].

Treatment Potential of UASB Process
UASB technology has been effectively used for the treatment of a wide range of wastewaters but is generally inhibited by incomplete biodegradability of complex wastewaters [10,28].
The UASB technology has been found to be very effective for the treatment of wastewater with a high content of carbohydrates.Carbohydrate rich organic wastewater, such as starch or canning industry wastewater is easily digested by microbes and is thus a nutrient-rich starting material for anaerobic hydrogen production.Upflow anaerobic sludge blanket (UASB) reactor has therefore turned out to be one of the most popular designs for the treatment of wastewaters from food processing industries.Anaerobic reactors have the ability to withstand variations in wastewater quality and complete shutdown of reactor in off season [28,29].Fang analyzed the microscopic (SEM and TEM) examinations of biogranules sampled from different UASB reactors.The microbial distribution in granules was found strongly dependent on the thermodynamics of degradation and kinetics of particular substrates.Biogranules degrading carbohydrates had a layered distribution in which the surface layer was occupied by hydrolytic/fermentative acidogens; the mid-layer consisted of syntrophic colonies and interior layer was comprised of acetotrophic methanogens, whereas substrates having a ratelimiting hydrolytic/fermentative step did not have a layered structure and bacteria were distributed evenly.The results reveal that granules are developed through evolution process and not through random aggregation of suspended microbes [29].Moreover, as supported by experimental evidence biogranules degrading carbohydrates are more resistant than suspended sludge to the toxicity of hydrogen sulfide, heavy metals, and aromatic pollutants in wastewater [30].
About half the organic matter content in domestic wastewater is attributable to black water having a major portion of nutrients.High rate UASB reactors are popular and have been applied widely due to their ability to manage high OLRs, short HTRs, and low energy requirements [31,32].A number of full-scale plants are operational and several others are at present under construction, especially in tropical or subtropical areas [33,34].Table 3 summarizes the advantages and disadvantages of UASB reactor.Basic concept of UASB technique is to build up anaerobic sludge that has good settling characteristics [1] and that can hold highly active bacterial aggregation without the requirement for immobilization on a support material [35].About 60% of the fullscale anaerobic treatment plants operating worldwide are currently based on the UASB design model, treating a wide range of industrial wastewaters [7,8,36,37].About 793 UASB reactors are installed worldwide showing the widest application of this technology out of total of approximately 1229 other anaerobic applications which include anaerobic contact filter, Expanded Granular Sludge Bed (EGSB) reactor, hybrid reactor, and fluidized bed.About half of these installations (338 out of 793) are in tropical and subtropical regions [38].Table 4 shows the performance of UASB reactors as compared to other technologies.On the basis of overall low investment and O and M costs, satisfactory COD, BOD, and TSS removal, and energy generation potential, this technology is suitable as compared to other technologies especially in developing countries.New research studies in this area have proven the successful operation of this system to treat low strength wastewater [12,39].The concentration of COD in domestic wastewater is usually low and suspended solids are high along

Advantages of UASB technology
(1) Provides high removal efficiency even at high OLR and low temperature and therefore requires smaller reactor volume [11,70] (2) Simple construction and low operation and maintenance cost due to local availability of construction material and other parts [84] (3) Robustness in treatment efficiency and wide applicability from very small to very large scale [11,22] (4) Energy is generated as methane/hydrogen gas.Energy generated can be used to heat the boilers to reduce operation costs.Less energy demand when external temperature control is not required [17,18] (5) Less CO 2 emissions due to less energy requirement and additional energy production in the form of biogas that can be used to run the system [44] ( Low sludge production as compared to aerobic processes.The sludge produced is stabilized having good dewatering characteristics can be stored for extended time periods and reused as an inoculum for seeding UASB reactors [77] (7) Quick startup time (about one week) by means of granular anaerobic sludge as seed [49] (8) Ability to withstand organic shock loads [11,25] (9) Ability to treat sewage due to availability of macro-and micronutrients and stability of pH without addition of any chemicals [73,76] Disadvantages of UASB technology (10) Needs posttreatment as pathogens are not removed completely, except for helminthes eggs that are successfully entrapped in the sludge.Incomplete removal of nutrients and therefore needing posttreatment [21,85] Long startup time is required due to the slow growth rate of microorganisms in case activated sludge is not amply available [20] ( Odor, toxicity, and corrosion problem: H 2 S is generated in anaerobic digestion, particularly when there is high concentration of sulfate in the wastewater.The biogas needs further suitable handling to avoid bad odor.The top gas box and the deflector can be constructed from concrete with epoxy coating to prevent corrosion and gas leakage [85,93] (13) In cold regions temperature needs to be maintained within (15-35 ∘ C) to achieve steady state performance [5] (14) A considerable portion of biogas produced may be dissolved in the effluent whose recovery is needed [10,94] with low methane yield that requires initial hydrolysis to convert the suspended solids into soluble substrate.Hydrolysis is commonly the limiting step at low temperature setting.Therefore, the UASB reactors for domestic wastewater treatment having high level of suspended solids are practicable only at higher temperature which may require an outside heat source [32,40].Better process understanding and operational knowledge on granules structure have made it possible to apply high organic loads and resulted in reducing startup time and providing a more sustainable operation [40,41].Its performance in the world proves it to be a consistent and efficient system for wastewater treatment [1].

Resource Recovery Potential
Anaerobic technologies have the ability to recover the chemical energy of organic carbon in wastewater as methane, hydrogen, and electricity.In UASB reactor, solids are captured and organic matter is converted into biogas consisting largely of methane and carbon dioxide.Organically bound nitrogen is transformed to ammonium and sulfate is reduced to hydrogen sulfide.Moreover, sludge generation is low and sludge produced is highly stable with good dewaterability characteristics [42].Under aerobic conditions, there is a complete loss of biomass energy in low value heat during the oxidation of organic matter, whereas under anaerobic degradation process, the original energy of the biomass is not changed.About 13.5 MJ of energy is stored in one kilogram COD.Depending on the extent to which anaerobic conversion is permitted, this energy is captured in the form of methane, hydrogen, other gases, or liquid compounds such as alcohols.Energy recovery is an old process which exists as alcohol fermentation and is now renewed by the production of biofuels from organically rich substrates [43].In terms of carbon foot print, anaerobic wastewater treatment is more advantageous, based on the relative efficiency of the aerobic system (Table 2).In case of effluent having low strength (BOD less than 300 mg/L), aerobic process is favorable, as it generates less greenhouse gas.But, in case of wastewater having higher strength (BOD more than 300 mg/L) anaerobic treatment is more beneficial.The recovery of dissolved methane in effluents in an economic way makes anaerobic wastewater treatment favorable in reducing carbon foot print for all wastewater strengths [44,45].CH 4 produced in the reactor should always be used for energy production and methane dissociation in the effluent and sulfate reduction  must be examined.About 20% of the CH 4 produced is dissolved in the effluent at ambient temperature of 20 ∘ C and biogas having methane content of 70%.As a result the benefits of energy production are reduced significantly.Nonetheless, diffusion of methane is high at low temperatures (less than 18 ∘ C) and it can be removed easily by a stripping chamber or air diffusion [46].In case of high sulfate or sulfite content, sulfate reducing bacteria compete with the methanogens for methanogenic substrates and therefore the amount of methane generated is decreased [3].In the United States and other developed countries, methane generated from anaerobic digestion is combusted for energy production, at large treatment plants, and is at least flared and converted to CO 2 at smaller plants [47].Increased biogas production, efficient biogas usage, and in turn decreased addition of external fossil will lead to reduction of carbon footprint of a wastewater treatment plant [48].
Hydrogen produced in anaerobic fermentation is a clean, carbon-free energy carrier gas, a substitute for fossil fuel, that is considered to play a main role in the future hydrogen based economy due to its high energy yield (122 kJ/g).The major problem in hydrogen production from biological processes is the low production rates and hydrogen yield at a large scale.Present research on hydrogen fermentation is directed towards determining culture details, beneficial substrates, conditions affecting microbial conversions, sequential fermentation, combined fermentation, mixed culture fermentation, optimizing enrichment, and stability of acidogenic sludge and reaction kinetics [49,50].Acetate (HAc), butyrate (HBu), caproate (HCa), propionate (HPr), and lactate (HLa) make up the metabolic pathways involved in the acidogenesis process in the UASB reactor for hydrogen production.HAc, HBu, and HCa are the desirable metabolites by-products for hydrogen production pathways.On the other hand, HLa and HPr are undesirable and consuming hydrogen pathways [51].A number of studies have been done on the generation of biohydrogen from food processing industries' wastewater; however very few studies have been carried out on hydrogen production from domestic wastewater.Potential of hydrogen production is now being realized by using domestic wastewater in anaerobic reactors [52,53].Fernandes et al. [53] reported hydrogen yield of 200 ml H 2 /gCOD by using domestic sewage as substrate in anaerobic reactor.Similarly, Paudel et al. [52] reported hydrogen yields of 1.014 mol H 2 /mol glucose at 3 g COD/L day loading rate using synthetic domestic wastewater.The operational conditions play a crucial role in hydrogen production.A pH of about 5.0-5.5 is reported to favor hydrogen production as the intermediates produced in the reactor can form appropriate ratios and stimulate the metabolism of particular microbes responsible for hydrogen fermentation.For both high rate hydrogen production and organics removal, organic acids generated in effluent can be methanized in a methanogenic reactor after hydrogenic reactor and energy generated from hydrogen may be utilized in posttreatment units where anaerobic reactor is the core technology for sewage treatment [53].
Factors such as inoculum, temperature, OLR, HRT, and pH must be taken into account for optimal hydrogen production.Use of inoculum previously adapted to hydrogen production greatly enhances hydrogen rate and yield as compared to nonadapted one.In mixed culture, hydrogen producing bacteria may consume the hydrogen produced and they need to be removed to curtail hydrogen consumers and enrich hydrogen producers [52][53][54].Previous studies do not show an optimal range of HRT and OLR in fermentative hydrogen production due to varying environmental process parameters or ranges of the variables reported in literature.The OLR can be optimized only when the microbes are well acclimatized to the applied OLR.The optimum temperature for hydrogen production through dark fermentation depends on the type of hydrogen producing bacteria and carbon source used [55].Hydrogen producing bacteria belong to mesophilic and thermophilic groups and generally rate of hydrogen production increases with increase in temperature through thermophilic bacteria compared to mesophilic bacteria [56].
Mu and Yu [57] demonstrated that hydrogen could be generated continuously and steadily from an acidogenicgranule-based UASB reactor at varying concentration of substrate (5.33-28.07g-COD/L) and HRTs of 3-30 h, during treatment of sucrose-rich synthetic wastewater.They found that H 2 partial pressure in biogas decreased with increasing substrate concentration; however it was not affected by the change of HRT in a range of 6-22 h.The rate of hydrogen production increased with increasing substrate concentration but decreased with increasing HRT with a hydrogen yield in the range of 0.49-1.44mol-H2/mol glucose [47].
Furthermore, research is underway on directly producing electric current from electrons released in anaerobic fermentation.These electrons are scavenged at an anode and with the use of cathode under oxidizing conditions electric current is generated which can be used in decentralized plants directly without any further conversion process [58,59].

Posttreatment Requirements
The UASB reactors do not warrant the removal of remaining organic matter, nutrients, and pathogens.Therefore, posttreatment of anaerobically treated wastewater is usually required in order to improve the quality of effluent in accordance with the irrigation standards.This has been successfully done by conventional systems such as maturation ponds, waste stabilization ponds, polishing ponds, constructed wetlands, rotating biological contactors, moving bed biofilm reactor, downflow hanging sponge [60][61][62], and advanced oxidative processes (AOPs) [63,64].
Polishing ponds are natural systems mainly for removal of solids which have been effectively used for the posttreatment of upflow anaerobic sludge blanket (UASB) effluents.They are mainly used as maturation ponds for the removal of pathogens, nitrogen, and remaining organic matter from the UASB reactor effluent [65].The use of sequential anaerobicaerobic technology such as UASB-activated sludge for municipal wastewater treatment combines the benefits of both systems in an economical arrangement.The combined system is energy efficient, less sludge producer, and relatively less complex for domestic wastewater treatment as compared to other options.An added advantage is the biological oxidization of the dissolved methane; however, the overall greenhouse gas reduction will depend on the energy consumption of aerobic step [44,66].The UASB and posttreatment system can be applied consecutively or in an integrated manner.

Effect of Different Parameters on the Efficiency of UASB Reactor
The efficiency of UASB reactors is regulated by a large number of factors including wastewater characteristics, acclimatization of seed sludge, pH, nutrients, presence of toxic compounds, loading rate, upflow velocity ( up ), hydraulic retention time (HRT), liquid mixing, and reactor design that affect the growth of sludge bed [16,[67][68][69].

Effect of Temperature
Temperature considerably influences the growth and survival of microorganisms.Anaerobic treatment is possible at all three temperature ranges (psychrophilic, mesophilic, and thermophilic); however, low temperature generally leads to a decline in the maximum specific growth rate and methanogenic activity [39,70,71].Methanogenic activity at this temperature range is 10 to 20 times lower than the activity at 35 ∘ C, which requires an increase in the biomass in the reactor (10 to 20 times) or operating at higher sludge retention time (SRT) and hydraulic retention time (HRT) in order to achieve the same COD removal efficiency as that obtained at 35 ∘ C [3,67].It is argued that the decrease of temperature slows down the hydrolysis and decreases the maximum growth and substrate utilization rates [5].Singh et al. [72] treated municipal wastewater using a UASB system under low temperature conditions and reported 70% COD removal at 11 ∘ C and 30 to 50% at 6 ∘ C. Lew et al. [40] found a gradual decrease in COD removal efficiency as the temperature was decreased.They reported 82% COD removal at 28 ∘ C, 72% at 20 ∘ C, 68% at 14 ∘ C, and 38% at 10 ∘ C. Kalogo and Verstraete [73] also found that COD removal efficiency at temperature in the range of 10-15 ∘ C was lower than that of efficiency at 35 ∘ C.
Van Lier and Lettinga [74] studied the effect of transient temperature rise on the performance of a UASB reactor containing mesophilic microorganisms.There was an increase in the methane production with an increase in the temperature due to the accelerated methanogenic activity.However, a sharp drop in the methane generation was noted at the reactor temperature exceeding 45 ∘ C because of a substantial decline in the activity of mesophilic granular sludge due to bacterial inactivation.Halalsheh et al. [75] treated high strength sewage (COD = 1531 mg/L) using a UASB pilot plant under subtropical conditions.The COD tot removal efficiencies were 62% and 51% in summer and winter, respectively, when the plant was operated at ambient temperature (18-25 ∘ C) and hydraulic retention time of 24 hours.

Effect of Hydraulic Retention Time (HRT)
Hydraulic retention time (HRT) is one of the most important parameters affecting the performance of the reactor especially in case of municipal wastewater [76,77].The upflow velocity ( up ) is directly related to HRT and plays an important role in entrapping suspended solids.A decrease in  up entails an increase in HRT, which boosts suspended solids' (SS) removal efficiency of the system [1,78,79].The COD removal efficiency of a UASB reactor also decreases at elevated upflow velocity because higher  up reduces the contact time between sludge and wastewater in addition to smashing of sludge granules and resultantly higher washout of solids [3,11,80].However, some scientists reported no distinct effect of HRT on the treatment efficiency of UASB reactor [22,77].The difference of opinion in scientific community is attributable to the difference in the reactor design, operating procedures, and range of HRT.Flow rate is also a key operational parameter that maintains the hydraulic retention time.In UASB process, if diameter of reactor will be too large then it may cause liquid channeling in the reactor leading to insufficient contact between the substrate and biomass.Therefore, large reactor will result in decreased biogas production and sludge washout due to insufficient mixing within the reactor.In contrast, comparatively more height may promote substrate mixing leading to proper contact of substrate with microorganisms resulting in more organic matter degradation and formation of biogas [81].
The upflow velocity ( up ) is helpful in providing adequate mixing of the substrate and biomass without channeling and maintaining the hydraulic retention time.The allowable limit of upflow velocity is 0.5-1.5 m/h as reported by different researchers.For the treatment of municipal wastewater in UASB reactor, researchers have reported successful operation at 0.31-0.426m/h  up at 3-4 h of HRT [32,75].

Effect of Organic Loading Rate (OLR)
OLR is the main parameter significantly affecting microbial ecology and functioning of UASB process.In case of sewage, the OLR usually is applied in the range of 1.0-2.0KgCOD/m 3 ⋅day [42,101].UASB reactor is preferred for its potential to treat wastewater having low content of suspended solids and gives higher methane yield [27].The reactors seeded with granular activated sludge can give high performance within a brief startup period and can also adapt quickly to increase of OLR [25].The effect of OLR on the performance of a UASB reactor depends on a number of factors which sometimes have a dissimilar effect, mostly contradictory, on the performance of UASB reactor [11].Researchers have reported an increase in the efficiency of high rate anaerobic reactors with increasing OLR [107].However, that increase is up to a certain OLR, beyond which there occurs sludge bed flotation and excessive foaming in the gasliquid-solids separator (GLSS); therefore a range of optimum OLR is usually recommended for a given temperature range and wastewater [108].Seghezzo [10] operated pilot-scale UASB reactors with OLR of 0.6 kgCOD/m 3 ⋅day (HRT of 6 hours and COD influent = 153 mg/L) and achieved maximum 63% COD removal efficiency at a low temperature of 17 ∘ C. Farajzadehha et al. [108] carried out a study on a lab-scale UASB reactor for determining the optimal organic loading rate and hydraulic retention time.Substrate used was fortified municipal wastewater at volumetric organic loadings of 3.6, 7.2, 10.8, and 14.4 kgCOD/m 3 ⋅day and temperature of 30 and 20 ∘ C. The results indicated an optimal organic loading rate range of 7.2 to 10.8 kgCOD/m 3 ⋅day at both temperature conditions attaining COD removal efficiency of 85% and 73% in UASB reactor at 30 ∘ C and 20 ∘ C, respectively.Nitrate removal efficiency was about 80% at optimized organic loading rate and there was a 30% decrease in nitrate removal efficiency when OLR was increased to 14.4 kgCOD/m 3 ⋅day.Halalsheh [22] determined the performance of UASB reactors for treatment of strong domestic wastewater at OLR of approximately 1.5 kgCOD/m 3 ⋅day (COD influent = 1500 mg/L) with a high portion of suspended solids (about 80%) at HRT of 24 hours.Even at considerably long HRT, the reactor achieved a COD removal efficiency of only 62% in summer (25 ∘ C).The comparatively low efficiency was caused by the sludge washout.Higher than optimal OLR results in the accumulation of biogas in the sludge bed forming gas pockets that ultimately cause sludge flotation [25].Leitao [11] determined the effect of influent concentration, HRT, and OLR on the COD removal efficiency of UASB reactors employed to treat sewage.Eleven (11) pilot-scale UASB reactors were divided into three sets.In first set, five reactors were operated at constant HRT of 6 hrs and with different COD Inf (92-816 mg/L) and corresponding OLR ranging from 0.2 to 3.3 kgCOD/m 3 ⋅day.In second set, four reactors were operated with approximately the same COD Inf (∼800 mg/L) but different HRTs (1, 2, 4, and 6 hrs) and corresponding OLR ranging from 3.3 to 17.6 kgCOD/m 3 ⋅day.In third set, HRTs were same as second set but the COD Inf was modified (136-816 mg/L) to have approximately the same organic loading rate (OLR∼ 3.3 kgCOD/m 3 ⋅day) in the four reactors.Results revealed that although the reactors of third set were operated with same OLR (3.3 kg COD/m 3 ⋅day) and different HRTs and COD Inf , they resulted in completely different COD removal efficiency varying from 13 to 57%.According to Leitao [11], as OLR is dependent on wastewater strength, upflow velocity, and volume of reactor, it is thus also dependent on the applied HRT.Therefore, impact of OLR on reactor performance is not simple, as it is dependent on other parameters, which have opposing effects on the removal efficiency of UASB; that is, increasing the feed concentration from 98 to 818 mg/L (and hence OLR of 0.4 to 3.3 kgCOD/m 3 ⋅day) up to a certain limit (OLR of 1.2 kg COD/m 3 ⋅day) caused an increase in removal efficiency (50 to 64%), whereas increasing the flow rate (and therefore OLR from 3.3 to 17.6 kg COD/m 3 ⋅day) caused a decrease in total COD efficiency from 57 to 36%.
In the case of effluent having COD contents lower than 300 mg/L (OLR: 0.4 to 0.8 kgCOD/m 3 ⋅day), the COD removal efficiency of UASB reactors was low (50-53%).However, reactors showed maximum COD removal efficiency of 64% when the COD concentration of influent was around 300 mg/L (OLR 1.2 kgCOD/m 3 ⋅day).At higher influent COD concentration (300 to 816 mg/L) and corresponding OLR of 2.2 to 3.3 kgCOD/m 3 ⋅day, the COD removal remained in the range of 57 to 60%.A decrease in SS removal as COD efficiency from 97 to 90% of UASB reactors with an increase in influent COD concentration from 92 to 816 mg/L and in turn OLR from 0.4 to 3.3 kgCOD/m 3 ⋅day was reported [11].When the increase in OLR (0.4 to 3.3 kgCOD/m 3 ⋅day) is due to an increase in the influent COD contents, a sharp decrease in SS removal efficiency may occur.That decrease owes to SS washout caused by turbulence due to higher rate of gas production.When the increase in OLR is associated with decreasing HRT (increase of flow rate), a decline in SS removal efficiency may occur due to sludge washout and short contact time between sludge bed and substrate, which results in the malfunctioning of physical and biological processes taking place in the reactor.However, SS removal efficiency of the reactor decreases to less extent when increase in OLR is associated with increased SS contents of substrate [11].Miron [109] reported an increase in SS removal with an increase in OLR associated with higher influent SS.These differences among the research workers are due to use of primary sludge by Miron to increase OLR.Primary sludge is mainly comprised of settleable suspended solids which increased the SS removal efficiency and therefore reported higher SS removal [11,108,109].
Thus, in order to describe the performance of UASB for treatment of sewage, the OLR has to be examined along with HRT and/or COD Inf as advised by Mahmoud [3].It is however clear from these studies that increasing the OLR will cause a decrease in efficiency of suspended solids removal.However, at a certain OLR, the efficiency of UASB would be optimal in case of long HRT and high COD load of influent (up to a certain limit).When the solids removal efficiency in UASB is related to the OLR, it is important to differentiate between these parameters.It is therefore inferred that OLR is an insufficient design parameter to guarantee good performance of anaerobic reactors [42].

Effect of pH
The pH of an anaerobic reactor is especially important because methanogenesis process can proceed at a high rate only when the pH is maintained in the range of 6.3-7.8 [110].In the case of domestic sewage, pH naturally remains in this range because of the buffering capacity of the acidbase system (carbonate system), and addition of chemical is not required [1].The UASB reactors employed for sewage treatment in tropical and subtropical countries are reported to be extremely stable in terms of pH and buffering capacity [10-12, 14, 22, 24, 111].Improvement in both hydrolysis and acidogenesis rates is achieved when treating domestic wastewater using anaerobic reactor and pH 7 provides an optimal working environment for anaerobic digestion resulting into more than 80% TOC and COD removal [111].

Effect of Granulation
In UASB reactor, long HRTs have been found disadvantageous for the development of granular sludge [107,112].In contrast, very short HRTs lead to washout of biomass.Both situations are unacceptable for achieving optimum results from UASB reactor.Even though granulation has been considered essential for successful treatment of domestic wastewater in UASB reactors, these reactors are found to be effective even without granules.The formation of granules in startup is helpful in shortening startup time [41,113,114].The high performance of the UASB reactor is based on the formation of an active sludge in the lower part of the reactor.The development of sludge bed occurs by the accumulation of incoming suspended solids and bacterial growth under specific conditions, due to the natural aggregation of bacteria in flocs and evolution of granules in the form of layered structure [29,115].These granules are not washed out from the reactor during operation of UASB.The diameter of the granulated sludge particles has been found in the range of 1.0 to 3.0 mm [8,[116][117][118].The granular suspensions have greater settling velocities (20-80 mh −1 ) as compared to upflow velocities ( up = 0.1-1.0mh −1 ).Therefore, substantial quantity of biomass can accumulate in the reactor.Consequently, a high sludge loading rate (SLR) could be applied (up to 5 gCODgVSS −1 day −1 ) with a relatively short HRT, less than 4 hours [25].Formation of activated sludge is important, in either granular or flocculent form, in the reactor which ensures adequate removal efficiency even at high OLR [27].

Effect of Mixing
Mixing offers effective contact time to microbes and wastewater, decreases hurdles of transfer of mass, lowers the growth of repressive by-products, and provides uniform environmental conditions.If mixing is not proper, the main process rate will be hampered by pockets of substrate at separate digestion stages, consequently leading to pH and temperature changes at every stage [119].Mixing can be achieved mechanically or by recirculation of methane gas or slurry.A number of researchers have observed that significant mixing affects the working of anaerobic reactors.Mixing enhanced efficiency of anaerobic systems treating wastewater with high COD concentration; moreover the recirculation of slurry exhibited better results as compared to biogas recirculation and impeller mixing mode [120].
Mixing also provides increased biogas production as compared to unmixed digesters [120].Discontinuous mixing is beneficial over energetic mixing when it is applied in large municipal and farm waste digesters [119].Formation of sludge granules occurs due to fluidization.Vigorous mixing is not recommended as methanogens are less effective in these conditions [121].Similarly, Karim et al. [120] reported that mixing in startup period lowers the digester pH, resulting in unstable performance and prolonged startup period.

Conclusions and Future Research Areas
UASB has been successfully applied for the treatment of industrial wastewater with high organic content and municipal wastewaters with high COD load in countries having temperate climatic conditions.Depending on the sewerage system, the sewage wastewater stream may contain toxic substances.Buildup of this matter can affect reactor performance.Overall UASB technology has been found successful for treating domestic wastewater.Therefore these reactors should be installed on priority basis in small communities and towns especially in developing countries with suitable climate conditions.In sewage treatment, the modeling of anaerobic digestion and obtaining higher yield of hydrogen from domestic wastewater have been active research areas in the last few years.The modification of UASB reactor, sequential use of UASB reactor with activated sludge/sequencing batch reactor/septic tank, flash aeration, two-stage UASB reactor/anaerobic filter/hybrid reactor, use of cosubstrate, posttreatment of UASB reactor for pathogen removal and reuse options, kinetics reactions and transfer of mass in anaerobic granular sludge, generation of high methane content, sludge profiling of UASB reactor at various OLRs, modeling for anaerobic granulation, and hydraulics and performance evaluation have been some of active research areas in this field.Modeling of UASB for performance evaluation will be very useful in directing future research on UASB system for direct treatment of wastewater.

Table 1 :
Characteristics of sewage in different cities of the world.

Table 3 :
Advantages and disadvantages of UASB reactor technology.

Table 4 :
Performance comparison of UASB with other wastewater treatment technologies.