The Correlation and Regression Analysis of Physicochemical Parameters of River Water for the Evaluation of Percentage Contribution to Electrical Conductivity

Ten different water samples were collected from Ratuwa River and its tributaries. )e laboratory test was carried out by standard procedures (APHAmethods), and the measured values were compared with the Nepal standard recommended by Nepal Drinking Water Quality Standards (NDWQS). Statistical analysis also had been used to calculate the correlation coefficients and to plot the regression equations of various parameters with electrical conductivity. )e aim of the study was to identify the parameters that affect the electrical conductivity and evaluate the percentage contribution of these parameters. )e correlation matrix shows that color, total dissolved solids (TDS), chloride (Cl), fluoride (F), total phosphorus (TP), total alkalinity (TA), calcium (Ca), magnesium (Mg), sodium (Na), and dissolved oxygen (DO) have a significant effect on the electrical conductivity (EC). Among these parameters, TDS has the highest contribution (39.65%) followed by total alkalinity (23.5%), total hardness (19.9%), chlorine (6.5%), and calcium (5.5%) ions, respectively. However, color, TP, fluoride, and DO have almost 1.45% contribution to the electrical conductivity.


Introduction
River water is an important source to provide the daily need of the fresh water for drinking and other purposes [1,2].However, such sources of fresh water are gradually contaminated due to domestic, municipal, and industrial effluents and severely damaging the equilibrium condition between the environment and aquatic system.Contaminated water is a great concern all over the world as it is related to the health hazards [3].
e cheapest and best option to examine the pollution status of water is to calculate its electrical conductivity, and its value depends upon the concentration of impurities, mobility, and temperature of the solution [4][5][6][7][8].For the industrial application, distilled water is categorized into ultrapure, pure, and regular depending upon the portion of impurities, [9] and it should have a fairly constant range of conductivity for a particular purpose.
erefore, any significant change in conductivity from the desired limit is also an indication of possible contamination so that precautions can be taken by monitoring it regularly.e conductivity measurement is also fairly used in leak detection, clean in place (CIP), desalination, detection of the interface of two liquid, and safety measure in the fuel industry.It also demonstrates the relationship between soil conditions, drought, rainfall, and other environmental situations that affect the production of crops [10,11].
Nepal being a Himalayan country, numerous investigations have been carried out all over the country to access the quality of water by analyzing the physicochemical and microbial parameters of the major river basins [12][13][14][15][16][17][18].In recent years, statistical tools such as correlation and regression analyses also have been extensively used for evaluating, monitoring, and predicting the concentration of impurities in water [19][20][21][22][23][24].But, no such works have been obtained in the literature, especially in Jhapa-based rivers of eastern Nepal.ese rivers are still untouched for the scientific purposes (research).
e Ratuwa River and its tributaries are one of them of which even physicochemical analysis has not been conducted to access the water quality.Similarly, Damak is one of the rapidly growing cities where an industrial park is going to be established near a bank of Ratuwa in around 960 acres so that such type of work will be useful for the future purpose.In the present work, we are not only analyzing and interpreting the hydrochemical parameters in terms of their chemical properties, but also evaluating the percentage contribution to the electrical conductivity of Ratuwa River using statistical techniques.Such type of work is very useful to recognize the contaminants without detailed investigation of physicochemical parameters and makes it easy and simple in the water treatment process.[25].Damak shares its eastern and western boundaries with Ratuwa and Mawa rivers, northern boundary with Siwalik Hills of Ilam district, and southern boundary with the intersection of Ratuwa River and Mawa River.Ratuwa River and its tributaries, namely, Haat Khola, Dipeni, Betani, and Dhukure, were selected for the sample collection to highlight the pollution status of water.Among these perennial rivers, Ratuwa originates from the Siwalik Hill and rest of them originates from wetlands a few kilometers away from the sample collection sites.Dipeni and Haat Khola pass through the city collecting the domestic and municipal wastes, whereas Betani and Dhukure Khola (river) pass outside the city collecting the agricultural wastes.

Materials and Methods
In the first phase, the sample was collected on the 11th of May 2017 and second phase data were also collected on the 16th of April 2018 from the same spot.e sampling sites and the location of the study area are shown in Figure 1.
First, the sampling bottles (one-liter plastic bottle) were rinsed before sampling, sealed tightly after collection, and labeled in the spot.e samples were taken by pumping from a depth of 5-10 cm below to avoid contamination from the surface of the river basin [26] and were brought to Nepal Batawaraniya Sewa Kendra, Biratnagar for the laboratory test where forty-nine different parameters had been measured from standard procedures (APHA methods).In the first phase, the measured parameters were color, turbidity, pH, electrical conductivity (EC), temperature, total solids (TS), total dissolved solids (TDS), total suspended solids (TSS), ammonia, chloride (Cl), residual chlorine, fluoride (F), sulphate, sulphide, silica, nitrite, nitrate, total nitrogen, total phosphorus (TP), soluble phosphorus, total hardness (TH), calcium hardness, acidity, carbon dioxide, total alkalinity (TA), aluminum, arsenic, cadmium, calcium (Ca), total chromium, hexavalent chromium, cobalt, iron, lead, manganese, nickel, magnesium (Mg), oil and grease, potassium, sodium, zinc, BOD, COD, dissolved oxygen (DO), total plate count, total coliform, faecal coliforms, faecal Streptococcus, and cyanide, respectively.In the second phase, color, pH, EC, TDS, TSS, chloride, fluoride, sulphate, nitrate, TP, TH, TA, calcium, iron, manganese, magnesium, potassium, sodium, and DO were measured.Among these, result of twelve physicochemical parameters (EC and strongly correlated parameters with EC) and their statistical summaries with units and measuring techniques are depicted in Tables 1 and 2, respectively.

Data Analysis.
To get an idea about the parameters that influence the electrical conductivity of water, firstly, the correlation matrix of 49 parameters was constructed (not shown).From the matrix, tentative parameters that affect the EC were identified keeping the significant level below 10%.On the basis of this result, again, five water samples were taken from the same sites as we had taken previously and measured only nineteen selected parameters to control the risk of misleading results and conclusion.Finally, a correlation matrix was again constructed from these parameters which were significantly correlated with EC with a significant level below 10% (p < 0.1) and it is shown in Table 3. From the given data, a linear best fit was drawn between EC and other in which EC was considered as an independent variable and other as a dependent variable.With the help of slope and intercept in the linear best fit line, a linear regression equation was formulated for each parameter.e linear regression equation and its percentage contribution to EC are shown in Table 4.

Results and Discussions
3.1.Physicochemical Analysis.Among the measured parameters, only significantly correlated parameters with the electrical conductivity were discussed and the measured value was compared with the Nepal standards.
Color is one of the most important physical parameters of water, and it is due to the presence of dissolved impurities and waste materials.e color of water ranged from 0.78 to 7 Hazen with standard deviation (SD) of 2.02.e minimum value was in Ratuwa in the first phase and maximum in the second phase of Haat Khola.According to NDWQS guideline, the color of water of 5 Hazen is more desirable but color till 15 Hazen is acceptable for drinking purpose [27].
e average value of electrical conductivity in Ratuwa, Haat Khola, Dipeni, Betani, and Dhukure was 171.1, 472.5, 308.9, 225.9, and 123.7 µs/cm, respectively.e higher values of Haat Khola and Dipeni showed the excessive presence of the domestic and municipal wastes.However, all these values were within the maximum concentration limit (1500 µs/cm) prescribed by NDWQS.
Total dissolved solids are natural pollutants in the river water and it imparts the color, total alkalinity, and conducting nature of water.e most desirable value of TDS is 500 mg/L.However, the optimum value of 1000 mg/L is acceptable according to NDWQS guideline.e maximum and minimum TDS were recorded in Haat Khola (256.5 mg/L) and Dhukure (59.95 mg/L) during the first phase of our sample 2 Journal of Chemistry collection.A higher value of TDS is generally harmful to those who are a victim of kidney and heart diseases [28].e chloride ion is an important constituent and found in water from natural as well as anthropogenic activities.Average value of chloride content of ve river samples ranged between 9 mg/l to 42.99 mg/L.ese values are relatively low as compared to the Nepal standard (250 mg/L) and show that water is less contaminated by the chloride.e main sources of phosphorus in water are agricultural fertilizers, manure, organic wastes, and industrial e uent, and it enters into the water from soil erosion, urban, and agricultural settings.It provides the nutrient for the growth  Journal of Chemistry of algae and aquatic plants.However, there is no recommended value of phosphorus for drinking purpose in Nepal.Among the five rivers, Haat Khola contained the highest average amount of total phosphorus (1.25 mg/L) followed by Dipeni (0.67 mg/L).It may be due to the domestic and municipal waste in the water.e lowest amount was observed in the Dhukure Khola (0.085 mg/L).e hardness of water is caused by the bicarbonate, sulphate, and chloride of calcium and magnesium.e average value of total hardness ranged from 23 to 116 mg/L, and these average values showed that Ratuwa, Dipeni, and Haat Khola were moderately hard but Betani and Dhukure Khola were moderately soft.Total hardness in Haat Khola may be due to a mixing of domestic and municipal effluents whereas total hardness level in the Ratuwa River may be due to the accumulation of the natural salts from soils and rocks [29].Among the calcium and magnesium content, calcium concentration is relatively higher than that of magnesium.
e average calcium concentration ranged from 11.41 to 33.82 mg/L with SD 10.55 while magnesium from 1.82 to  Dissolved oxygen (DO) measures the amount of oxygen dissolved naturally in water.For aquatic ecosystem and metabolic activities of microorganism, DO value should be greater than 4 mg/L and for drinking purpose 6 mg/L.In our samples, average DO value was 4.72, 2.57, 3.98, 4.35, and 4.77 mg/L in Ratuwa, Haat Khola, Dipeni, Betani, and Dhukure, respectively.Comparatively higher value of EC and low DO content in Haat Khola and Dipeni showed the mild pollution river as a result of organic or inorganic waste in a low volume of water [30].
Sodium content of all analyzed samples varied from 4.43 mg/L to 16.38 mg/L with SD of 3.69.e maximum sodium content was observed in Haat Khola (13.06 mg/L) and Betani (10.86 mg/L).
e variation in chlorine also followed the same pattern in these rivers but their concentrations (Na + and Cl − ) did not satisfy 1 : 1 ratio in water.It indicates that there is not only one source of origin of these ions.Natural and anthropogenic sources are responsible for the higher concentration of Cl − irrespective to Na + concentration [19].
Fluoride is naturally present in river water and its presence prevents dental caries and bone damage.But its higher concentration causes dental fluorosis, and even higher concentration causes skeletal fluorosis [31].In all samples, fluoride concentration was above the detection limit of an instrument (>0.05 mg/L) and within the permissible limit (0.5-1.5 mg/L) of the Nepal standards prescribed by NDWQS except in Dhukure where it was within the detection limit (0.05 mg/L).
Total alkalinity is a measure of all alkaline substances dissolved in the water such as carbonates, bicarbonates, and hydroxides.e highest value of TA was observed in Haat Khola (139.4 mg/L) followed by Dipeni (117.4 mg/L) and Betani (110.5 mg/L), respectively.e least value was recorded in Dhukure where its concentration was only 22.1 mg/L.e permissible range is not recommended for drinking purpose in Nepal.

Percentage Contribution to EC.
A correlation matrix of twelve parameters, namely, color, EC, TDS, chloride, fluoride, total phosphorus (TP), total hardness (TH), total alkalinity (TA), calcium, magnesium, sodium, and dissolved oxygen (DO), was constructed and is shown in Table 3. e correlation coefficient always measures the relationship between the dependent and independent variables.If the correlation coefficient is +1 or −1, it shows the perfect linear relationship between the variables.
Total phosphorus also depicted the fairly positive linear correlation with TH (r � 0.916) and Ca (r � 0.792) and a moderate positive correlation with TA (r � 0.754), Ca (r � 0.699), and Na (r � 0.745).A moderate negative correlation of TP with DO indicates that TP content always reduces the DO value of water.e reason for it is as follows: the total phosphorus always provides the nutrient for the growth of algae and aquatic plants.If an excess of phosphorus is present in the water, algae and aquatic plants will grow wildly and consume large amounts of DO causing eutrophication.Total hardness showed the significant correlation with TA (r � 0.864), Ca (r � 0.766), and Mg (r � 0.817) while total alkalinity also demonstrated the significant correlation with Ca (r � 0.707) and Mg (r � 0.556).
After developing the correlation coefficient matrix, scatter diagrams were constructed and shown in Figures 2  and 3, respectively.In these figures, EC was plotted along X-axis and others dependent parameters along Y-axis.e slope, Y-interception and adjusted R 2 of each linear best fit were shown in the inset of each figure.Similarly, a regression equation was also formulated from the pairs and depicted in Table 4. e adjusted R 2 tells us whether the repressor is good at explaining or predicting the values of the dependent variable in the samples [32].e higher the value of adjusted R 2 , the better is the fit of data points and more useful the regression variables.e adjusted R 2 between TDS and EC is 1, and it indicates that all data points perfectly fit in the given regression line and significance of the equations was indicated by p value.
To calculate the percentage contribution to EC, a known value of EC (500 µs/cm) was considered in the linear regression equations shown in Table 4 and a dependent variable (Y-value), as well as its percentage contribution to EC were calculated [33].e percentage contribution of each parameter to EC is presented in Figure 4. e result showed that the highest percentage contribution of TDS to EC was 39.6%.Second and third highest contribution was 23.5% and 19.9% by total alkalinity and total hardness, respectively.To obtain the known value of EC, calcium and chloride also contributed 5.48% and 6.50%, respectively, while sodium showed 2.2% and magnesium, 1.3% contribution.e higher contribution to EC by these independent parameters might be due to a high degree of correlation and higher concentration in water.
e least percentage contribution was obtained by the parameters such as color, TP, F, and DO.Each of these parameters had less than 1% contribution, and it was due to either a low degree of correlation or less concentration in water.

Conclusion
e result of the measured twelve physicochemical parameters of Damak-based rivers demonstrates that they are in the permissible range of drinking water except the value of color in Haat Khola (7 Hazen).Comparatively higher

Figure 1 :
Figure 1: Location of the study site showing the sampling points inserted in the map of Nepal.

Table 1 :
Analysis of physicochemical parameters of di erent rivers in Damak with their units.

Table 3 :
Correlation matrix of twelve different physicochemical parameters.

Table 4 :
Linear regression equations of different physicochemical parameters and EC.
Figure 2: Correlation of color, TDS, chloride, ouride, total phosphorus, and total hardness with electrical conductivity.6Journal of Chemistry value of EC and other parameters and low value of DO indicates the mild pollution of Haat Khola and Dipeni.A very strong correlation of EC with color, TDS, Cl, F, TP, TH, Ca, and Mg and moderate correlation with TA, Na, and DO are observed.When all these parameters are treated independently for the calculation of percentage contribution to EC, it is found that TDS has the highest contribution (39.6%) while TA has second highest contribution (23.5%) followed by TH (19.9%).Calcium and chloride ions also have a signi cant contribution and