High-Voltage Electric Field-Assisted Thawing of Frozen Tofu : Effect of Process Parameters and Electrode Configuration

Applying high-voltage electric eld (HVEF) to some foodmaterials has been shown to increase the thawing rate. To investigate the eect of process parameters and electrode conguration in high-voltage electric eld system, we took the frozen tofu as the research object and investigated the inuence of the dierent voltages, electrode conguration, and electrode distances on thawing process. e thawing time, center temperatures, and loss rate of samples were measured. e results showed that the thawing time of frozen tofu decreases with the increase of voltage and the thawing time has a great relevance with conguration and distance of electrodes. e electric parameters have a major eect on thawing loss and thawing time when center temperatures of frozen tofu are from −2°C to 0°C. is work provides clues and experimental basis for the further application of high-voltage electric eld thawing technology.


Introduction
awing of frozen materials is an important component of food product processing.Many technologies are widely used in food thawing industry, such as cold and warm water thawing, still air thawing, refrigerator thawing, and so on.However, all have their own shortcomings, which greatly a ects the products' subsequent production and market competitiveness, including higher color deterioration and weight loss, longer thawing time, and decreased nutritional value of the thawed products.erefore, it is signi cant to explore the new thawing technology.
High-voltage electric eld (HVEF) thawing is an important nonthermal food processing method that has received considerable attention lately [1], and it is predominantly used to thaw food products in academia, including pork [2,3], tuna sh [4][5][6][7], chicken [8], rabbit meat [9], shrimps [10], common carp [11], tofu [12], and apple tissue [13].Compared to traditional thawing methods, high-voltage electric eld thawing has many advantages [3,6,10] such as reduced thawing time, food quality preservation, microbial growth inhibition, reduced energy consumption, and so on.e thawing can be carried out using either AC or DC high voltages.Multipoint and plate electrode systems are e cient in accelerating thawing of freeze materials [10].When the thawing temperature was set at −3 °C, the thawing time of frozen chicken under high-voltage electric eld was 2/3 the time taken for thawing meat [14].
e HVEF treatment signi cantly shortened the thawing time of frozen pork tenderloin meat, and thawing time was reduced to 2/3 that of the control and reduced the total microbial counts in thawed frozen meat by 0.5-1 log CFU/g [2].Mousakhani-Ganjeh et al. reported that the high-voltage electric eld could increase susceptibility of tuna sh to lipid oxidation due to ozone generation [6].In previous studies, we studied the thawing characteristic of frozen tofu from the thawing rate, center temperatures, thawing loss, speci c energy consumption, and mathematical models under AC electric eld at di erent voltages and found that the thawing rate of frozen tofu was notably greater in the high-voltage electric eld system when compared to control [12].Both linear and quadratic models were the best mathematical models [12].
Despite the detail with which these studies treat the problem, few studies have systematically and comprehensively reported on the effects of process parameters and electrode configuration under different high-voltage electric field thawing conditions.
To further investigate the effect of process parameters and electrode configuration for optimizing and improving the thawing efficiency in high-voltage electric field system, we studied the thawing characteristic of frozen tofu.To accomplish this, high-voltage electric field thawing characteristics and quality of frozen tofu were studied, including the thawing electric voltage, thawing time, center temperatures of samples, thawing loss, and the configuration of the electrodes under different thawing conditions.

Experimental Facility.
e thawing experimental equipment is shown schematically in Figure 1 [12,15].e HVEF thawing equipment consists of three parts: highvoltage power system, thawing system, and control system.e thawing system consists of a high-voltage electrode, the configuration of which is multiple sharp pointed needles or wires or metallic plate, and a fixed horizontal grounded metallic plate.e electrode gaps between the high-voltage electrodes and the grounded electrode can be adjusted.e high-voltage electrodes were connected to a high-voltage power system that can supply alternating current (AC) or direct current (DC) high voltage.e frequency of the AC electric field was 50 Hz.e high-voltage power (YD(JZ)-1.5/50,made in Wuhan, China) was connected to a voltage regulator (KZX-1.5 KVA, made in Wuhan, China) as control system, with an adjustable voltage ranging from 0 to 50 kV for AC or 0 to 70 kV for DC by a controller.e grounded electrode was an 80 cm × 40 cm rectangular stainless steel plate.
e ambient temperature and relative humidity in thawing process were both measured.
e voltage and current of HVEF system were measured by a voltmeter (made in Ningbo, China) and an amperometer (made in Ningbo, China), respectively.e metal needle which the length is 20 mm and diameter is 1 mm was evenly arranged on the needle plate electrode (64 cm × 40 cm) by stainless steel wire.e distance between two needle electrodes was 40 mm.e dimensions of high-voltage electrode with plate or wires were a 64 cm × 40 cm.e distance between two stainless steel wires was 40 mm.All the samples were spread in a single layer on the grounded plate electrode at random.e center temperature of samples was measured by a temperature sensor.

Preparation and Treating of Tofu.
e soft tofu was purchased from a local market near Inner Mongolia University of Technology, Hohhot, China.e fresh soft tofu was sliced into sheet about 3.5 cm × 3.5 cm × 3.5 cm using a knife and immediately frozen at −18 °C in a refrigerator (Hisense BCD-197T, made in Qingdao, China).e frozen samples were stored at −18 °C until use.

Measurement of awing Process and awing Time.
We conducted three experimental conditions to further investigate the effect of process parameters and electrode configuration for optimizing and improving the thawing efficiency in high-voltage electric field system.e voltage, electrode configuration, and discharge gap were investigated, respectively.
Firstly, the frozen tofu was thawed under DC electric field with multiple needles-to-plate electrode at different voltages.
Secondly, the configuration of high-voltage electrodes was multiple needles-to-plate, multiple wires-to-plate, or multiple plate-to-plate electrodes, respectively, under AC electric field.
e corresponding discharge gap between the high-voltage electrodes and the grounded electrode was 100 mm.e corresponding voltage was 28 kV.e same thawing experiments were also investigated under DC electric field.
Lastly, the discharge gap between the emitting point and the grounded electrode each time at 8 cm, 9 cm, 10 cm, 11 cm, and 12 cm with multiple needles-to-plate electrode for AC electric field was changed, and the corresponding voltage was 20 kV. e sample thickness is lower than the discharge gap to make better use of the nonuniform electric field and the ion wind.And this would better reduce energy consumption during thawing.
e thawing temperature was 20 ± 1 °C, the relative humidity was 30 ± 5%, and the ambient wind speed was 0 m/s.e center temperature of frozen tofu samples determined by a digital thermometer (made in China) and recorded at 5 min intervals during the thawing process.In this paper, "center temperature" refers to the temperature at the geometric center of frozen tofu samples, where heat takes longest to penetrate.awing was continued until the center temperature of the frozen tofu sample reached 10 °C.e time required to raise the center temperature from −10 °C to 10 °C was determined as thawing time.awing experiments were independently performed three times in this study and the average was taken.In each thawing experiment, the sample is placed at the same position on the grounded electrode to keep the consistency of the experiment.It is found in other studies that both the center and surface temperatures of samples increased rapidly with the increase of applied voltages and have some similar characteristics [3].So, we only measured the center temperature of frozen tofu to investigate the effect of process parameters and electrode configuration.
ere are some electrochemical reactions during the thawing process, which may lead to electrode corrosion and metal release.When the electrode was designed, we use a protective paint on the surface to prevent electrode oxidation and corrosion.And there is no contact here between the electrode and the material during the thawing process.en, the effect of electrochemical reaction on the thawing process is very small.We think that it can be ignored.
e thawing rate of material samples (g/s) was calculated using the following equation: thawing rate � weight of frozen tofu(g) thawing time of frozen tofu(s) . (1)

Measurement of Evaporation Loss, awing Loss, and Drip
Loss.Evaporation loss, thawing loss, and drip loss were measured by weighing the frozen and thawed material samples before and after the removal of surface water according to (2), (3), and (4), respectively: evaporation loss � (weight of the frozen tofu) − (weight of the thawed tofu before removing surface water) weight of the frozen tofu thawing loss � (weight of the frozen tofu) − (weight of the thawed tofu after surface water removal) weight of the frozen tofu , drip loss � thawing loss − evaporation loss.
Experiments were independently performed three times in this study and the average was taken.

Statistical Analysis.
Single-factor analysis of variance was used to calculate the evaporation loss, thawing loss, and drip loss between the frozen tofu under alternating electric field and without electric field (control).e evaporation loss, thawing loss, and drip loss between different electric field were also calculated using single-factor analysis of variance.
e differences in thawing loss are considered statistically significant when p < 0.05.

awing Time and awing Rate Analysis. Figure 2(a)
shows the effect of different voltages on the thawing time and thawing rate under DC electric field.e frozen tofu was thawed at 20 °C with a fixed discharge gap of 10 cm under DC electric field.
e thawing times at 0 kV (the control), 4 kV, 8 kV, 12 kV, 16 kV, 20 kV, 24 kV, and 28 kV under DC electric field were 200 min, 155 min, 150 min, 145 min, 140 min, 135 min, 130 min, and 125 min, respectively.In other words, the thawing times treated with the high-voltage electric field were significantly shortened than those of the control (0 kV).With the increase of voltage, the thawing time decreased.e high-voltage electric field could obviously accelerate the thawing rate of tofu samples compared to that of the control, and increasing the voltage had a major effect on the enhancement of the thawing rate.ese results agree with those studies which reported enhancement in thawing rate with increase of applied voltage [3,4].At present, it is generally believed that the main reason of thawing rate accelerating is the generation of corona wind which was produced by the high-voltage electric field.e samples are put on a metal plate (cathode), while electrodes are mounted in some distance to the cathode and will form a corona when the electric circuit is closed [16].Under the HVEF system, the corona wind produced impinges on the material and disturbs the liquid part of the thawing tofu, leading to thawing enhancement.e corona electrode with small curvature radius could form a nonuniform HVEF and realize a corona discharge in the gas-filled gap.e corona wind would be higher when the configuration of the electrodes is adopted in the form of needle-to-plate or wire-plate rather than the plate-to-plate form.us, the configuration of the electrodes would impact on the thawing rate of frozen food.
Figure 2(b) shows the effect of different configuration of the electrodes on the thawing time and thawing rate.e thawing times for the electrodes of plate-to-plate under DC Journal of Food Quality electric field, plate-to-plate under AC electric field, wires-toplate under DC electric field, wires-to-plate under AC electric field, needles-to-plate under DC electric field, and needles-to-plate under AC electric field were 180 min, 155 min, 175 min, 120 min, 125 min, and 75 min, respectively.We can see that the thawing time under AC electric field was shortened significantly when compared to that under DC electric field.
e thawing time for the electrodes of needles-to-plate under AC electric field was the shorter than that under the other experimental conditions.And the thawing time for the electrodes of plate-to-plate under DC electric field was the longer than that under the other experimental conditions.e thawing rate of tofu samples treated with AC electric field is higher than that treated with DC electric field.From Figure 2(b) shows that the thawing rate of frozen tofu under AC electric field is higher than that under DC electric field when they have the same voltage and electrodes.erefore, another high-voltage electric field thawing mechanism is possible besides corona wind under the AC electric field.As water molecules are highly polar, they orient themselves in the direction of the electric field, which in-turn would lead to the conversion of 4 Journal of Food Quality electrical energy into mechanical energy, thereby forcing water molecules out of the material [12].Relevant study also showed that the high eld intensity can reorient water molecules in ice and modify the crystal morphology, so that freezing is inhibited, leading to the acceleration of the thawing process [17].Figure 2(c) that the e ect of di erent discharge gaps on the thawing time and thawing rate.e thawing times for the discharge gaps of 8 cm, 9 cm, 10 cm, 11 cm, and 12 cm were 70 min, 80 min, 85 min, 90 min, and 100 min, respectively.As discharge gap increased, the thawing time increased.ese results are similar with what has been found in other studies [3,4].e electric eld strength decreases with the increase of discharge gap.e magnitude of the electric wind velocity was proportional to the electric eld strength.So, decreasing the discharge gap had a major e ect on the enhancement of the thawing rate.

Center Temperatures Analysis.
e center temperatures exposed to high-voltage electric eld are shown in Figure 3.
e results indicate that within the rst 10 min, the center the needles-to-plate electrodes under AC electric eld.e corresponding discharge gap between the high-voltage electrodes and the grounded electrode was 100 mm.e corresponding voltage was 28 kV.For each treatment, means with di erent lower case letters are signi cantly di erent (p < 0.05).(c) e evaporation loss, thawing loss, and drip loss of tofu under di erent electrode distances.e discharge gap was 8 cm, 9 cm, 10 cm, 11 cm, and 12 cm with multiple needles-to-plate electrode for AC electric eld, respectively.e corresponding voltage was 20 kV.For each treatment, means with di erent lower case letters are signi cantly di erent (p < 0.05).

6
Journal of Food Quality increased slowly between −2 °C and 0 °C in all experimental conditions.Most of the thawing time is longer in the temperature range (−2 °C-0 °C) than that of other temperature range.e center temperature range (−5 °C to −1 °C) is often taken as the zone of maximum ice crystal formation in the food freezing industry [14].When the center temperature of frozen tofu is from −2 °C to 0 °C, high-voltage electric field treatment exerts its maximum effect.is result coincides with other studies [5].ere is a considerable variation about the thawing time exposed to HVEF at −2 °C-0 °C when the voltages, the configuration of the electrodes and electrode distances changed.e thawing time under AC electric field is higher than that under DC electric field.ere have different thawing time for the different configuration of the electrodes.e thawing time with needles-to-plate electrodes is faster than that with the other configuration of the electrodes.
e thawing time with wires-to-plate electrodes is higher than that with plate-to-plate electrodes.us, the electric parameters have a major effect on the thawing time when center temperatures of frozen tofu are from −2 °C to 0 °C.As can be seen from Figure 3, the results indicate that the rising rate of center temperature increased with rise in voltage from −2 °C to 10 °C.When the corona wind blows to the surface of the material, the electrical conductivity of the frozen tofu would changes.
en, this would affect HVEF-assisted thawing process.e change of electrical conductivity of samples starts to apply HVEF.However, the effect on thawing is relatively small in the initial stage.e thawing effect increased with rise in the change of electrical conductivity of samples.When the electrical conductivity rises above a certain value, the thawing effect is more remarkable.And there is a great correlation between high voltage and the change of electrical conductivity of samples.

Evaporation Loss (EL), awing Loss (TL), and Drip Loss
(DL) Analysis.Evaporation loss, thawing loss, and drip loss of the tofu samples were measured with different thawing parameters in our study.Effect of thawing parameters on the evaporation loss, thawing loss, and drip loss of tofu was given in Figure 4. Water holding capacity has a great relevance with thawing loss.Water holding capacity is high when thawing loss of the material samples is low [2].e results showed that with the increase of voltages, evaporation loss increased.e evaporation rate of material samples treated with high-voltage electric field significantly accelerated compared to that of the control when the voltage is higher than a specific value [18][19][20].Drip loss and thawing loss of frozen tofu were very close under DC electric field.Drip loss and thawing loss treated with high-voltage electric field were less than that with the control.e thawing loss under AC electric field is higher than that under DC electric field.Using the different configuration of the electrodes, the thawing rate is highest with needles-to-plate electrodes and is lower with plate-to-plate electrodes than that under the other experimental conditions.e evaporation loss decreased with increasing applied electrode distance.But drip loss and thawing loss increased with increasing applied electrode distance.As can be seen, the results indicate that changes in voltage make no great difference to thawing loss under DC electric field.e configuration of the electrodes and electrode distances has significant effects on thawing loss.In other words, water holding capacity of tofu is improved at certain experimental conditions using HVEF thawing.
e thawing process can cause the structural changes of the tofu, and this change may lead to an increase in textural properties such as hardness, springiness, cohesiveness, and gumminess, which may match with consumer preferences for harder and springier tofu [21].In the thawing process, the thawing time has a major effect on thawing loss.e thawing time of frozen tofu was significantly shortened under high-voltage electric field than that of the control.

Conclusion
awing under HVEF treatment significantly improved the thawing rate and shortened the thawing time of frozen tofu.As the voltage was enhanced, thawing rate increased.Voltage has a little effect on thawing loss under DC electric field.But the configuration of the electrodes and electrode distances has a major effect on thawing loss.In other words, the quality of thawed samples was improved by the HVEF thawing technology.We hope that this study can promote the industrial application of high-voltage electric field in the thawing field and attract more studies about HVEF thawing technique.

Figure 2 :
Figure2:(a) awing time and thawing rate of frozen tofu in different voltages.e frozen tofu was thawed at 20 °C with a fixed discharge gap of 100 mm under DC electric field.e thawing voltage was 0 kV (the control samples), 4 kV, 8 kV, 12 kV, 16 kV, 20 kV, 24 kV, or 28 kV, respectively.(b)awing time and thawing rate of frozen tofu in different electrodes.Plate (DC): the plate-to-plate electrodes under DC electric field, plate (AC): the plate-to-plate electrodes under AC electric field, W-P (DC): the wires-to-plate electrodes under DC electric field, W-P(AC): the wires-to-plate electrodes under AC electric field, N-P(DC): the needles-to-plate electrodes under DC electric field, and N-P(AC): the needles-to-plate electrodes under AC electric field.e corresponding discharge gap between the high-voltage electrodes and the grounded electrode was 100 mm.e corresponding voltage was 28 kV (c) awing time and thawing rate of frozen tofu in different electrode distances.e discharge gap was 8 cm, 9 cm, 10 cm, 11 cm, and 12 cm with multiple needles-to-plate electrode for AC electric field, respectively.e corresponding voltage was 20 kV.

Figure 3 :Figure 4 :
Figure3: (a) Changes in temperature during thawing of frozen tofu in di erent voltages.e frozen tofu was thawed at 20 °C with a xed discharge gap of 100 mm under DC electric eld.e thawing voltage was 0 kV (the control samples), 4 kV, 8 kV, 12 kV, 16 kV, 20 kV, 24 kV, or 28 kV, respectively.(b) Changes in temperature during thawing of frozen tofu in di erent electrodes.e corresponding discharge gap between the high-voltage electrodes and the grounded electrode was 100 mm.e corresponding voltage was 28 kV.(c) Changes in temperature during thawing of frozen tofu in di erent electrode distances.e discharge gap was 8 cm, 9 cm, 10 cm, 11 cm, and 12 cm with multiple needles-to-plate electrode for AC electric eld, respectively.e corresponding voltage was 20 kV.