In this study, a fast, simple, precise, and sensitive hydrophilic interaction liquid chromatography (HILIC) method was established for simultaneous determination of free amino acids in three different varieties of duckweed including
Amino acids are kinds of nitrogenous components that play vital and diverse roles in metabolism and have attracted significant attention in food, feedstuff, and alimentary supplements. Amino acids are basic units and important ingredients of proteins and involved in the progress of biosynthesis for glycoprotein, porphyrins, neurotransmitters, polyamines, and nitric oxide [
The family of Lemnaceae colloquially known as duckweed has been consumed as human food since long [
There are several methods reported in literature for analysis of free amino acids. In general, the FAAs are extracted with solvents water, formic acid, hydrochloric acid, or ethanol, followed by filtration or centrifugation before analysis [
In present study, a feasible and reliable UPLC-QTRAP-MS/MS method was developed and validated for quantitative analysis of 24 underivatized FAAs simultaneously. Multivariate statistical analysis was employed to assess the differences in the profiles of FAAs among three species of duckweed. This simple and fast analytical method would be used for quality control of duckweeds.
59 batches of duckweed were collected from different regions in China as shown in Table
Information of duckweeds from different regions.
Sample | Species | Site |
---|---|---|
SP1-7 |
|
Yangzhou City of Jiangsu Province |
SP8-9 |
|
Yancheng City of Jiangsu Province |
SP10-11 |
|
Nanjing City of Jiangsu Province |
SP12-13 |
|
Huaian City of Jiangsu Province |
SP14-17 |
|
Lianyungang City of Jiangsu Province |
SP18-19 |
|
Linyi City of Shandong Province |
SP20-21 |
|
Hefei City of Anhui Province |
SP22-23 |
|
Hangzhou City of Jiangsu Province |
SP24-25 |
|
Baoding City of Hebei Province |
LP1-11 |
|
Yangzhou City of Jiangsu province |
LP12-16 |
|
Huaian City of Jiangsu province |
LA1-3 |
|
Yangzhou City of Jiangsu Province |
LA4-8 |
|
Yancheng City of Jiangsu Province |
LA9-14 |
|
Nanjing City of Jiangsu Province |
LA15-16 |
|
Lianyungang City of Jiangsu Province |
LA17-18 |
|
Linyi City of Shandong Province |
Chromatographic analysis was performed on a Shimadzu LC-30 ultrahigh performance liquid chromatography system (Shimadzu, Japan), which consisted of a communication bus module (CMB-20A), a vacuum degasser, binary gradient pumps (LC-30AD), an autosampler (SIL-30A), and a column oven (CTO-30A) coupled with an AB Sciex QTRAP 5500 (AB SCIEX, USA). A Waters XBridge Amide column (2.1 mm × 150 mm, 2.5
The mass spectrometry assay was performed on a triple quadruple mass spectrometer equipped with an electrospray ionization source. The spectra were recorded under positive-ion type with the multiple reaction monitoring (MRM) mode. The capillary voltage was 5500 V, and desolvation gas temperature was 550°C. The curtain gas was 35 psi with both nebulizer and drying gas of 55 psi. Nitrogen was used as source gas with purity more than 95% and collision gas with purity over 99.999%. The 24 components of amino acids were optimized in the tuning mode with mass only to obtain the mass of the precursor and product ion, the best declustering potential (DP), and collision energy (CE), respectively. The details are shown in Table
The precursor ions, product ions, declustering potential, and collision energy of amino acids.
Number | Compound | Precursor ion | Product ion | DP (V) | CE (V) |
---|---|---|---|---|---|
1 | Tyr | 182.0 | 91.1 | 60 | 37 |
2 | Ala | 90.0 | 44.1 | 50 | 14 |
3 | Glu | 148.1 | 84.0 | 60 | 21 |
4 | Phe | 166.1 | 120.1 | 60 | 19 |
5 | His | 156.1 | 110.3 | 80 | 20 |
6 | Ile | 132.1 | 86.1 | 50 | 14 |
7 | Asp | 134.1 | 74.0 | 50 | 19 |
8 | Lys | 147.0 | 84.1 | 60 | 23 |
9 | Gly | 76.1 | 29.9 | 40 | 16 |
10 | Val | 118.1 | 71.9 | 50 | 15 |
11 | Cit | 176.2 | 70.0 | 60 | 31 |
12 | GABA | 104.1 | 86.8 | 50 | 14 |
13 | Cys2 | 241.1 | 73.9 | 80 | 40 |
14 | Pro | 116.0 | 70.1 | 60 | 22 |
15 | Trp | 205.1 | 146.0 | 50 | 24 |
16 | Leu | 132.1 | 86.1 | 50 | 14 |
17 | Arg | 175.2 | 70.2 | 90 | 30 |
18 | Cys | 122.1 | 59.0 | 40 | 32 |
19 | Hpro | 132.1 | 86.1 | 60 | 20 |
20 | Met | 150.1 | 103.9 | 60 | 14 |
21 | Thr | 120.1 | 74.1 | 40 | 14 |
22 | Asn | 133.1 | 74.0 | 60 | 21 |
23 | Gln | 147.0 | 84.1 | 60 | 23 |
24 | Ser | 106.0 | 60.1 | 60 | 15 |
Typical MRM chromatograms of the 24 amino acids.
Stock solutions of individual standards were made by dissolving accurately weighed components in 0.5% formic acid at a concentration of 0.2 mg/mL approximately and stored at 4°C before use. The mixed working solutions of all the standards were diluted with 0.5% formic acid solution to a series of appropriate concentrations immediately before analysis and used to attain the calibration curves.
Each sample powder was weighed 0.2 g accurately and transferred into a 25 mL stainless tube with 6 stainless beads with a diameter of 0.6 mm. Then, the sample was extracted in a high-throughput tissue-grinding apparatus with vibrating frequency of 70 kHz for 120 s with 15 mL 0.5% formic acid. The sample solution was centrifuged at 15000 rpm for 10 min, and then the supernatant was screened through a 0.22
For each reference substance, the calibration curve was confirmed by linear regression of the peak area versus concentration. The limits of detection (LOD) and limits of quantification (LOQ) were determined by diluting the standard solution till the signal-to-noise ratios were about 3 and 10, respectively. Precision was determined by intraday and interday variability. The intraday variability was conducted by determining the same standard solution in six replicates on the same day. The interday variability was conducted by determining the same solution for three consecutive days. The relative standard deviation (RSD) values were calculated to denote the precision. Stability of the sample solution was analysed by peak areas of analytes at 0, 2, 4, 8, 12, and 24 h at room temperature. To evaluate the repeatability of the developed method, the same sample was analysed in six replicates and variations were expressed as RSD. The recovery test was performed to assess the accuracy of the method. For the test, a known amount of the 24 standard components was spiked into a certain amount (0.1 g) of sample. Then, the spiked sample was extracted and analysed as described. The recovery rate was calculated by using the following formula: recovery (%) = (measured amount − original amount)/spiked amount × 100%. Six replicates were performed for the recovery test.
The quantitative analyses of acquired data were processed by MultiQuant 3.0.2 (AB SCIEX, USA). Multivariate statistical analysis including PCA and OPLS-DA was used to classify the sample. SIMCA-P 14.1 (Umetrics AB, Sweden) was used to perform statistical analysis for the data of 59 batches of duckweed.
Twenty-four minerals of each environment water where duckweed lived were quantified by ICP-MS. Total nitrogen and phosphorus of the water body were measured according to the alkaline potassium persulfate digestion method GB11894-89 and the ammonium molybdate spectrophotometric method GB11893-89 published by the State Bureau of Technological Supervision of China. Total proteins of each duckweed were determined by the Kjeldahl method. Each sample of duckweeds was hydrolyzed in acidic conditions, and then total amino acids were quantified by the HPLC-MSMS method mentioned above.
Free amino acids can dissolve in methanol, ethanol, water, diluted acid, and so on. In order to acquire an efficient extraction strategy of amino acids from duckweed, variables of the extraction process were investigated. The solvents (methanol, ethanol, water, 0.1% formic acid, and 0.5% formic acid), methods (heating reflux, ultrasonication, and tissue-grinding), and time (20, 30, and 40 min for ultrasonication; 60, 90, and 120 s for tissue-grinding) were studied. The results showed that the amount of amino acids had no significant difference between the ultrasonication time of 30 min in 0.5% formic acid and tissue-grinding time of 120 s in 0.5% formic acid. The amounts of extraction by other methods were not more than that by these two modalities. From the viewpoint of working efficiency, the tissue-grinding method was much more time-saving than the ultrasonication method by the extracting time of 120 s versus 30 min.
Chromatographic conditions were optimized to attain a satisfactory separation for amino acid especially for isomeric molecules. Columns (Waters XBridge Amide, 2.5
The results demonstrated that the peak shape of amino acids became good when the concentration of formic acid rose, but retention time would be shortened significantly especially if some formic acid was complemented into the acetonitrile. The buffers such as ammonium formate increased the resolution degrees of amino acids. Better retention times and separations were observed by conducting analysis on Waters Amide column than on Agilent Poroshell 120 HILIC column, and the best chromatograms were gained with temperature controlled at 20°C. Especially for the isomers of leucine and isoleucine, baseline separation was successfully achieved when using the mobile phase consisting of acetonitrile-10 mM ammonium formate with 0.2% formic acid. Under other abovementioned conditions, these two isomeric amino acids were not well separated reciprocally.
Primary experiments were conducted to acquire the best mass spectrometry conditions of amino acids individually. For this purpose, each reference component of amino acids with the concentration of approximate 100 ng/mL was delivered into the mass spectrometer directly via a syringe pump with the velocity of 7
As shown in Table
Calibration curves, LODs, and LOQs for the 24 compounds.
Number | Compound | Calibration curve |
|
Linear range (ng/mL) | LOQ (ng/mL) | LOD (ng/mL) |
---|---|---|---|---|---|---|
1 | Tyr |
|
0.9984 | 1.16–5780 | 0.92 | 0.46 |
2 | Ala |
|
0.9969 | 5.30–5300 | 3.71 | 1.24 |
3 | Glu |
|
0.9960 | 6.41–2564 | 4.49 | 1.50 |
4 | Phe |
|
0.9965 | 0.99–992.0 | 0.79 | 0.40 |
5 | His |
|
0.9962 | 5.57–5570 | 3.90 | 1.30 |
6 | Ile |
|
0.9957 | 1.07–1074 | 0.86 | 0.43 |
7 | Asp |
|
0.9963 | 5.43–5430 | 3.80 | 1.27 |
8 | Lys |
|
0.9980 | 5.53–5530 | 3.87 | 1.94 |
9 | Gly |
|
0.9990 | 0.94–4680 | 0.75 | 0.37 |
10 | Val |
|
0.9962 | 1.11–1112 | 0.89 | 0.44 |
11 | Cit |
|
0.9980 | 1.01–5070 | 0.81 | 0.41 |
12 | GABA |
|
0.9981 | 1.10–5490 | 0.88 | 0.44 |
13 | Cys2 |
|
0.9978 | 1.09–5440 | 0.87 | 0.44 |
14 | Pro |
|
0.9981 | 1.02–510.0 | 0.82 | 0.41 |
15 | Trp |
|
0.9977 | 1.24–2476 | 0.99 | 0.50 |
16 | Leu |
|
0.9974 | 1.01–1012 | 0.81 | 0.40 |
17 | Arg |
|
0.9974 | 5.96–5960 | 4.17 | 1.39 |
18 | Cys |
|
0.9988 | 1.20–6012 | 0.96 | 0.48 |
19 | Hpro |
|
0.9977 | 0.95–1892 | 0.76 | 0.38 |
20 | Met |
|
0.9984 | 1.05–5250 | 0.84 | 0.42 |
21 | Thr |
|
0.9957 | 4.46–4460 | 3.12 | 1.56 |
22 | Asn |
|
0.9967 | 1.20–5980 | 0.96 | 0.48 |
23 | Gln |
|
0.9961 | 0.93–4650 | 0.74 | 0.37 |
24 | Ser |
|
0.9977 | 5.09–5090 | 3.56 | 1.19 |
Precision, stability, repeatability, and accuracy of the investigated analytes (
Number | Compound | Precision | Stability RSD (%) | Repeatability RSD (%) | Accuracy | ||
---|---|---|---|---|---|---|---|
Intraday RSD (%) | Interday RSD (%) | Recovery (%) | RSD (%) | ||||
1 | Tyr | 1.36 | 1.85 | 0.94 | 3.05 | 98.56 | 1.33 |
2 | Ala | 2.60 | 3.31 | 3.47 | 3.09 | 102.49 | 2.18 |
3 | Glu | 2.07 | 2.56 | 2.10 | 2.11 | 106.94 | 3.76 |
4 | Phe | 0.66 | 1.03 | 2.60 | 1.66 | 97.23 | 4.03 |
5 | His | 1.07 | 3.30 | 1.58 | 1.17 | 98.32 | 2.20 |
6 | Ile | 0.97 | 3.21 | 3.82 | 0.71 | 99.09 | 3.57 |
7 | Asp | 1.96 | 3.98 | 2.42 | 1.84 | 97.86 | 2.36 |
8 | Lys | 2.70 | 3.51 | 1.44 | 0.99 | 102.30 | 3.02 |
9 | Gly | 1.66 | 2.65 | 3.43 | 3.74 | 104.48 | 3.07 |
10 | Val | 2.42 | 2.89 | 3.30 | 0.83 | 107.51 | 1.52 |
11 | Cit | 1.47 | 1.88 | 3.12 | 3.86 | 99.14 | 2.21 |
12 | GABA | 3.14 | 2.32 | 2.27 | 0.82 | 102.54 | 3.41 |
13 | Cys2 | 2.53 | 4.27 | 3.24 | 1.49 | 96.60 | 1.27 |
14 | Pro | 1.21 | 3.39 | 1.09 | 2.09 | 101.94 | 3.20 |
15 | Trp | 0.37 | 3.93 | 1.36 | 1.39 | 99.11 | 3.46 |
16 | Leu | 1.50 | 3.90 | 3.40 | 1.50 | 95.27 | 3.61 |
17 | Arg | 2.72 | 4.15 | 3.72 | 1.45 | 102.37 | 1.79 |
18 | Cys | 2.82 | 3.63 | 1.16 | 0.53 | 103.65 | 3.82 |
19 | Hpro | 3.05 | 2.23 | 0.87 | 2.38 | 97.55 | 3.91 |
20 | Met | 1.57 | 3.02 | 1.26 | 0.52 | 104.89 | 2.14 |
21 | Thr | 2.11 | 2.13 | 1.77 | 3.01 | 95.97 | 3.00 |
22 | Asn | 2.93 | 2.85 | 1.48 | 3.68 | 105.35 | 2.95 |
23 | Gln | 0.97 | 1.87 | 1.22 | 2.72 | 102.33 | 2.16 |
24 | Ser | 2.71 | 2.86 | 0.98 | 1.48 | 97.44 | 1.88 |
The developed UPLC-MS/MS method was applied for simultaneous determination of 24 free amino acids in 59 batches of three species of duckweed. All the contents are displayed in Table
Contents of 24 free amino acids, total amino acids, total proteins (
Sample | Tyr | Ala | Glu | Phe | His | Ile | Asp | Lys | Gly | ||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Free | Total | Ratio | Free | Total | Ratio | Free | Total | Ratio | Free | Total | Ratio | Free | Total | Ratio | Free | Total | Ratio | Free | Total | Ratio | Free | Total | Ratio | Free | Total | Ratio | |
SP1 | 3.16 | 6259.48 | 0.0505 | 6.95 | 10680.18 | 0.0651 | 25.27 | 16774.31 | 0.1506 | 0.44 | 9597.56 | 0.0046 | 1.52 | 4255.26 | 0.0357 | 0.38 | 8532.07 | 0.0045 | 9.31 | 11169.49 | 0.0834 | 11.95 | 7164.09 | 0.1668 | 3.33 | 8764.89 | 0.0380 |
SP2 | 6.73 | 6161.25 | 0.1092 | 65.17 | 10089.59 | 0.6459 | 54.61 | 25366.04 | 0.2153 | 0.95 | 10729.73 | 0.0089 | 4.43 | 3888.26 | 0.1139 | 1.35 | 10094.12 | 0.0134 | 56.68 | 15862.58 | 0.3573 | 21.28 | 10090.45 | 0.2109 | 8.38 | 8695.46 | 0.0964 |
SP3 | 39.72 | 6115.87 | 0.6495 | 173.11 | 9881.22 | 1.7519 | 96.43 | 15463.41 | 0.6236 | 7.97 | 8667.06 | 0.0920 | 22.26 | 3824.22 | 0.5821 | 11.90 | 7226.86 | 0.1647 | 69.12 | 12387.34 | 0.5580 | 112.90 | 8153.11 | 1.3847 | 128.27 | 6293.65 | 2.0381 |
SP4 | 29.21 | 6251.45 | 0.4673 | 157.69 | 12424.62 | 1.2692 | 49.52 | 22986.51 | 0.2154 | 5.01 | 10467.29 | 0.0479 | 32.80 | 3569.79 | 0.9188 | 11.14 | 8392.28 | 0.1327 | 170.28 | 17175.49 | 0.9914 | 137.48 | 10225.20 | 1.3445 | 78.17 | 9626.14 | 0.8121 |
SP5 | 5.58 | 6257.00 | 0.0892 | 29.04 | 13866.45 | 0.2094 | 34.93 | 23518.46 | 0.1485 | 0.86 | 11427.08 | 0.0075 | 6.41 | 4312.95 | 0.1486 | 0.96 | 9753.24 | 0.0098 | 16.39 | 18654.70 | 0.0879 | 21.25 | 11094.76 | 0.1915 | 5.53 | 10174.01 | 0.0544 |
SP6 | 27.42 | 6245.68 | 0.4390 | 273.35 | 12005.84 | 2.2768 | 49.09 | 18787.83 | 0.2613 | 2.55 | 10284.72 | 0.0248 | 45.46 | 3424.75 | 1.3274 | 13.55 | 8465.53 | 0.1601 | 132.84 | 15752.94 | 0.8433 | 139.27 | 8608.52 | 1.6178 | 77.36 | 7010.22 | 1.1035 |
SP7 | 39.61 | 5884.11 | 0.6732 | 229.20 | 12123.87 | 1.8905 | 71.86 | 18146.81 | 0.3960 | 4.90 | 8585.01 | 0.0571 | 46.17 | 4088.80 | 1.1292 | 11.22 | 7231.96 | 0.1551 | 94.84 | 14833.85 | 0.6393 | 230.52 | 7636.34 | 3.0187 | 65.35 | 5417.04 | 1.2064 |
SP8 | 5.71 | 6124.81 | 0.0932 | 38.00 | 9734.57 | 0.3904 | 53.14 | 17640.33 | 0.3012 | 1.17 | 8527.84 | 0.0137 | 4.56 | 3751.52 | 0.1216 | 1.40 | 7266.84 | 0.0193 | 42.82 | 14315.75 | 0.2991 | 22.77 | 7392.06 | 0.3080 | 5.63 | 7832.50 | 0.0719 |
SP9 | 13.90 | 6054.66 | 0.2296 | 120.58 | 9953.18 | 1.2115 | 70.75 | 16620.79 | 0.4257 | 3.68 | 8384.54 | 0.0439 | 18.01 | 3496.99 | 0.5150 | 5.25 | 6745.54 | 0.0778 | 94.10 | 11606.55 | 0.8107 | 73.99 | 6323.41 | 1.1701 | 21.63 | 7899.86 | 0.2738 |
SP10 | 16.70 | 6097.17 | 0.2739 | 118.61 | 13272.16 | 0.8937 | 74.72 | 25451.33 | 0.2936 | 3.72 | 11056.99 | 0.0336 | 28.19 | 4110.01 | 0.6859 | 4.41 | 9671.14 | 0.0456 | 36.11 | 17757.11 | 0.2034 | 70.81 | 8546.75 | 0.8285 | 25.55 | 7505.72 | 0.3404 |
SP11 | 23.10 | 6004.10 | 0.3847 | 205.08 | 11155.38 | 1.8384 | 67.31 | 26088.53 | 0.2580 | 10.26 | 9883.88 | 0.1038 | 61.35 | 3238.60 | 1.8943 | 14.23 | 8891.25 | 0.1600 | 56.14 | 15601.46 | 0.3598 | 133.66 | 8386.70 | 1.5937 | 87.43 | 9088.41 | 0.9620 |
SP12 | 9.38 | 6127.68 | 0.1531 | 93.06 | 9760.92 | 0.9534 | 51.58 | 26516.14 | 0.1945 | 7.60 | 7997.37 | 0.0950 | 31.87 | 3300.60 | 0.9656 | 4.09 | 9260.26 | 0.0442 | 225.20 | 12413.46 | 1.8142 | 74.86 | 5684.24 | 1.3170 | 24.76 | 6908.86 | 0.3584 |
SP13 | 93.61 | 6130.06 | 1.5271 | 195.69 | 8956.55 | 2.1849 | 52.85 | 16377.44 | 0.3227 | 23.33 | 7668.17 | 0.3042 | 46.78 | 2889.26 | 1.6191 | 14.85 | 7650.07 | 0.1941 | 4.76 | 10136.24 | 0.0470 | 135.91 | 6635.82 | 2.0481 | 73.19 | 5663.18 | 1.2924 |
SP14 | 19.98 | 6120.49 | 0.3264 | 161.86 | 13337.15 | 1.2136 | 96.09 | 15114.77 | 0.6357 | 5.94 | 10678.41 | 0.0556 | 26.03 | 4189.64 | 0.6213 | 6.56 | 9933.71 | 0.0660 | 29.20 | 13225.32 | 0.2208 | 102.55 | 11141.92 | 0.9204 | 34.50 | 7778.37 | 0.4435 |
SP15 | 1.13 | 6057.44 | 0.0187 | 19.99 | 13617.28 | 0.1468 | 17.01 | 21045.25 | 0.0808 | 0.62 | 11056.19 | 0.0056 | 9.77 | 4213.32 | 0.2319 | 0.30 | 8452.34 | 0.0035 | 0.95 | 12630.34 | 0.0075 | 17.70 | 8018.34 | 0.2207 | 6.64 | 6872.17 | 0.0966 |
SP16 | 61.29 | 5884.22 | 1.0416 | 280.92 | 12551.36 | 2.2382 | 46.82 | 20775.77 | 0.2254 | 19.27 | 10311.88 | 0.1869 | 54.60 | 3881.89 | 1.4065 | 20.18 | 9290.92 | 0.2172 | 112.81 | 13791.37 | 0.8180 | 158.24 | 7293.79 | 2.1695 | 52.41 | 8307.13 | 0.6309 |
SP17 | 0.89 | 5938.26 | 0.0150 | 23.96 | 10170.30 | 0.2356 | 46.90 | 15656.17 | 0.2996 | 0.42 | 10562.87 | 0.0040 | 2.49 | 5358.17 | 0.0465 | 1.11 | 10201.54 | 0.0109 | 2.95 | 12263.36 | 0.0241 | 13.08 | 8069.24 | 0.1621 | 4.44 | 5669.36 | 0.0783 |
SP18 | 12.28 | 5985.32 | 0.2052 | 157.75 | 13673.70 | 1.1537 | 81.27 | 15272.53 | 0.5321 | 15.58 | 10449.06 | 0.1491 | 28.93 | 4949.42 | 0.5845 | 10.04 | 9948.89 | 0.1009 | 85.38 | 20641.51 | 0.4136 | 79.39 | 11463.15 | 0.6926 | 29.19 | 6848.95 | 0.4262 |
SP19 | 45.66 | 5897.55 | 0.7742 | 182.98 | 12217.80 | 1.4977 | 87.43 | 20888.93 | 0.4185 | 28.84 | 10128.19 | 0.2847 | 48.02 | 4231.77 | 1.1347 | 20.85 | 9161.44 | 0.2276 | 10.22 | 14574.99 | 0.0701 | 83.12 | 9060.52 | 0.9174 | 34.17 | 6664.53 | 0.5127 |
SP20 | 28.03 | 5860.01 | 0.4783 | 241.91 | 9302.34 | 2.6005 | 46.17 | 14837.19 | 0.3112 | 23.18 | 7603.97 | 0.3048 | 64.10 | 3206.91 | 1.9988 | 14.80 | 6774.94 | 0.2185 | 109.03 | 11544.71 | 0.9444 | 164.87 | 6737.28 | 2.4471 | 73.15 | 6715.81 | 1.0892 |
SP21 | 2.75 | 6264.80 | 0.0439 | 55.55 | 10989.25 | 0.5055 | 91.79 | 22493.65 | 0.4081 | 0.84 | 9615.10 | 0.0087 | 23.80 | 4257.07 | 0.5591 | 1.00 | 8364.75 | 0.0120 | — | 11442.81 | — | 31.30 | 6932.76 | 0.4515 | 9.46 | 6910.06 | 0.1369 |
SP22 | 7.95 | 5839.15 | 0.1361 | 29.20 | 10343.17 | 0.2823 | 22.86 | 16692.99 | 0.1369 | 1.07 | 8957.29 | 0.0119 | 5.17 | 3585.28 | 0.1442 | 1.06 | 8011.79 | 0.0132 | 0.91 | 13590.39 | 0.0067 | 15.15 | 6361.56 | 0.2381 | 11.21 | 7059.65 | 0.1588 |
SP23 | 6.86 | 6265.35 | 0.1095 | 68.62 | 11510.74 | 0.5961 | 100.28 | 20064.52 | 0.4998 | 2.53 | 9584.25 | 0.0264 | 32.22 | 3573.51 | 0.9016 | 1.84 | 7753.50 | 0.0237 | 33.31 | 15126.56 | 0.2202 | 42.69 | 7622.22 | 0.5601 | 11.21 | 8763.05 | 0.1279 |
SP24 | 2.86 | 6318.22 | 0.0453 | 121.06 | 11508.52 | 1.0519 | 133.69 | 16946.16 | 0.7889 | 1.99 | 9477.13 | 0.0210 | 25.81 | 4474.44 | 0.5768 | 2.27 | 7926.91 | 0.0286 | 47.91 | 15776.45 | 0.3037 | 30.02 | 7939.55 | 0.3781 | 10.04 | 8867.42 | 0.1132 |
SP25 | 43.24 | 5967.19 | 0.7246 | 261.74 | 11024.75 | 2.3741 | 53.23 | 21609.69 | 0.2463 | 16.89 | 9293.80 | 0.1817 | 64.22 | 4222.87 | 1.5208 | 20.92 | 9560.51 | 0.2188 | 162.59 | 18624.39 | 0.8730 | 209.44 | 7299.84 | 2.8691 | 63.50 | 7917.96 | 0.8020 |
LP1 | 15.93 | 5971.26 | 0.2668 | 66.50 | 12151.90 | 0.5472 | 30.75 | 24958.79 | 0.1232 | 11.80 | 10826.10 | 0.1090 | 35.08 | 4061.19 | 0.8638 | 4.88 | 9319.60 | 0.0524 | 108.30 | 14896.35 | 0.7270 | 47.16 | 8613.79 | 0.5475 | 14.76 | 9583.97 | 0.1540 |
LP2 | 38.97 | 5921.55 | 0.6581 | 140.81 | 11584.07 | 1.2155 | 63.83 | 21969.13 | 0.2905 | 15.15 | 9814.43 | 0.1544 | 30.41 | 4647.49 | 0.6543 | 2.61 | 8750.97 | 0.0298 | 59.96 | 16728.80 | 0.3584 | 134.72 | 7842.84 | 1.7177 | 30.64 | 6109.14 | 0.5015 |
LP3 | 10.25 | 6287.19 | 0.1630 | 65.63 | 11013.29 | 0.5959 | 55.16 | 18366.66 | 0.3003 | 2.58 | 10992.07 | 0.0235 | 16.14 | 3302.54 | 0.4887 | 0.65 | 8567.75 | 0.0076 | 28.45 | 14101.74 | 0.2017 | 40.15 | 6593.31 | 0.6090 | 7.10 | 4632.59 | 0.1533 |
LP4 | 47.76 | 6004.07 | 0.7955 | 180.48 | 11148.05 | 1.6189 | 83.06 | 18093.89 | 0.4590 | 7.65 | 9312.27 | 0.0821 | 48.49 | 3515.42 | 1.3794 | 9.18 | 8187.52 | 0.1121 | 103.28 | 15513.40 | 0.6657 | 94.68 | 8007.43 | 1.1824 | 74.60 | 8640.35 | 0.8634 |
LP5 | 74.45 | 6011.02 | 1.2386 | 318.14 | 9785.11 | 3.2513 | 50.05 | 16830.48 | 0.2974 | 17.43 | 8490.88 | 0.2053 | 38.14 | 3571.09 | 1.0680 | 11.66 | 7900.11 | 0.1476 | 218.70 | 13106.01 | 1.6687 | 129.60 | 8308.91 | 1.5598 | 44.93 | 5574.15 | 0.8060 |
LP6 | 22.44 | 6404.35 | 0.3504 | 187.93 | 9320.51 | 2.0163 | 51.11 | 19093.97 | 0.2677 | 10.76 | 10746.47 | 0.1001 | 17.66 | 4145.39 | 0.4260 | 4.29 | 8189.38 | 0.0524 | 137.49 | 13879.01 | 0.9906 | 57.61 | 6486.39 | 0.8882 | 13.94 | 6180.53 | 0.2255 |
LP7 | 17.40 | 6413.54 | 0.2713 | 167.19 | 10950.61 | 1.5268 | 73.42 | 20616.00 | 0.3561 | 10.75 | 9606.73 | 0.1119 | 20.94 | 3464.43 | 0.6044 | 2.23 | 8717.93 | 0.0256 | 207.21 | 15567.27 | 1.3311 | 51.72 | 8761.56 | 0.5903 | 14.60 | 6213.59 | 0.2350 |
LP8 | 35.02 | 6285.44 | 0.5572 | 139.27 | 11569.10 | 1.2038 | 72.38 | 18381.09 | 0.3938 | 18.51 | 10138.80 | 0.1826 | 28.90 | 4434.85 | 0.6517 | 7.46 | 9558.72 | 0.0780 | 73.30 | 15357.08 | 0.4773 | 78.65 | 7886.32 | 0.9973 | 24.80 | 8792.99 | 0.2820 |
LP9 | 41.17 | 5951.60 | 0.6917 | 149.55 | 11075.37 | 1.3503 | 48.55 | 22021.01 | 0.2205 | 9.10 | 11619.61 | 0.0783 | 52.62 | 3058.17 | 1.7206 | 12.36 | 8587.29 | 0.1439 | 182.44 | 15444.00 | 1.1813 | 202.10 | 10583.78 | 1.9095 | 176.76 | 6207.16 | 2.8477 |
LP10 | 23.74 | 6012.91 | 0.3948 | 187.15 | 10077.16 | 1.8572 | 57.20 | 22847.31 | 0.2504 | 3.20 | 10674.83 | 0.0300 | 11.62 | 3881.87 | 0.2993 | 6.47 | 10514.94 | 0.0615 | 48.04 | 19845.70 | 0.2421 | 111.09 | 8283.89 | 1.3410 | 107.73 | 7557.95 | 1.4254 |
LP11 | 14.86 | 6110.90 | 0.2432 | 94.14 | 9256.17 | 1.0171 | 16.70 | 26967.52 | 0.0619 | 5.60 | 7780.40 | 0.0720 | 7.52 | 3541.02 | 0.2124 | 3.81 | 7300.09 | 0.0522 | 6.45 | 13678.56 | 0.0472 | 75.66 | 7596.11 | 0.9960 | 46.74 | 6276.56 | 0.7447 |
LP12 | 8.06 | 6259.52 | 0.1288 | 25.80 | 10729.35 | 0.2405 | 11.61 | 28848.73 | 0.0402 | 1.45 | 9609.21 | 0.0151 | 4.31 | 4095.51 | 0.1052 | 0.93 | 8635.84 | 0.0108 | 2.44 | 11847.35 | 0.0206 | 29.23 | 8736.23 | 0.3346 | 7.00 | 7434.59 | 0.0942 |
LP13 | 33.36 | 6270.49 | 0.5320 | 274.21 | 10059.65 | 2.7258 | 85.36 | 26195.21 | 0.3259 | 5.22 | 11114.42 | 0.0470 | 44.10 | 3500.71 | 1.2597 | 8.74 | 9771.06 | 0.0894 | 78.86 | 17064.92 | 0.4621 | 148.06 | 8026.46 | 1.8446 | 92.39 | 7807.71 | 1.1833 |
LP14 | 29.35 | 6070.40 | 0.4835 | 160.58 | 13818.32 | 1.1621 | 57.00 | 27399.97 | 0.2080 | 5.18 | 12682.81 | 0.0408 | 38.41 | 5535.27 | 0.6939 | 9.59 | 11826.29 | 0.0811 | 139.52 | 14718.19 | 0.9479 | 158.90 | 7779.67 | 2.0425 | 85.79 | 7101.48 | 1.2081 |
LP15 | 14.08 | 6310.67 | 0.2231 | 108.54 | 12226.01 | 0.8878 | 11.43 | 20415.24 | 0.0560 | 4.29 | 10235.21 | 0.0419 | 6.83 | 4786.65 | 0.1427 | 3.49 | 10163.51 | 0.0343 | 4.42 | 16813.25 | 0.0263 | 70.82 | 8454.76 | 0.8376 | 57.87 | 8826.59 | 0.6556 |
LP16 | 38.10 | 6308.99 | 0.6039 | 224.42 | 10920.13 | 2.0551 | 58.19 | 18915.27 | 0.3076 | 12.98 | 9252.57 | 0.1403 | 25.58 | 3501.54 | 0.7305 | 6.06 | 8361.97 | 0.0725 | 187.80 | 14243.86 | 1.3185 | 79.64 | 8381.28 | 0.9502 | 24.49 | 7349.44 | 0.3332 |
LA1 | 39.53 | 5844.15 | 0.6764 | 153.40 | 11080.16 | 1.3845 | 53.94 | 17900.19 | 0.3013 | 5.26 | 8940.22 | 0.0588 | 38.98 | 3436.65 | 1.1342 | 10.44 | 7739.17 | 0.1349 | 92.27 | 11396.06 | 0.8097 | 116.20 | 8514.88 | 1.3647 | 16.63 | 8366.72 | 0.1988 |
LA2 | 7.06 | 6163.58 | 0.1145 | 110.58 | 11359.90 | 0.9734 | 38.25 | 21707.93 | 0.1762 | 8.65 | 9105.55 | 0.0950 | 45.78 | 3419.43 | 1.3388 | 10.95 | 9140.90 | 0.1198 | 84.67 | 16077.78 | 0.5266 | 87.09 | 8669.05 | 1.0046 | 14.22 | 7375.81 | 0.1928 |
LA3 | 49.51 | 6127.32 | 0.8080 | 189.37 | 10480.08 | 1.8070 | 47.31 | 23969.23 | 0.1974 | 23.46 | 8251.42 | 0.2843 | 73.17 | 3213.54 | 2.2769 | 30.13 | 8053.11 | 0.3741 | 190.71 | 18067.61 | 1.0555 | 206.67 | 10157.90 | 2.0346 | 118.69 | 11012.41 | 1.0778 |
LA4 | 9.45 | 6231.43 | 0.1517 | 101.15 | 10764.28 | 0.9397 | 43.93 | 18445.78 | 0.2382 | 6.83 | 9106.47 | 0.0750 | 5.48 | 3968.13 | 0.1381 | 2.07 | 7183.48 | 0.0288 | 14.98 | 11095.78 | 0.1350 | 44.31 | 8400.33 | 0.5275 | 18.02 | 5093.33 | 0.3538 |
LA5 | 8.21 | 6468.09 | 0.1269 | 101.70 | 10782.93 | 0.9432 | 49.41 | 17857.46 | 0.2767 | 4.67 | 9378.50 | 0.0498 | 27.88 | 4320.50 | 0.6453 | 7.67 | 8732.91 | 0.0878 | 245.22 | 11348.94 | 2.1607 | 101.42 | 9642.82 | 1.0518 | 59.10 | 7589.10 | 0.7787 |
LA6 | 95.03 | 6441.94 | 1.4752 | 53.56 | 10969.89 | 0.4882 | 45.63 | 19113.36 | 0.2387 | 34.05 | 8826.04 | 0.3858 | 22.67 | 3432.16 | 0.6605 | 15.73 | 7873.91 | 0.1998 | 194.49 | 13395.53 | 1.4519 | 203.37 | 9453.82 | 2.1512 | 165.17 | 5220.68 | 3.1638 |
LA7 | 43.57 | 6390.61 | 0.6818 | 136.48 | 9697.57 | 1.4074 | 44.59 | 17227.16 | 0.2588 | 26.85 | 10617.54 | 0.2529 | 53.25 | 3342.84 | 1.5930 | 8.20 | 8078.65 | 0.1015 | 99.41 | 13308.10 | 0.7470 | 156.91 | 8444.81 | 1.8581 | 124.79 | 6172.25 | 2.0218 |
LA8 | 25.51 | 5969.99 | 0.4273 | 159.29 | 9551.72 | 1.6677 | 21.69 | 22493.72 | 0.0964 | 7.29 | 12500.69 | 0.0583 | 28.21 | 5068.31 | 0.5566 | 12.52 | 10500.36 | 0.1192 | 2.23 | 19936.15 | 0.0112 | 66.00 | 11282.00 | 0.5850 | 75.19 | 11732.39 | 0.6409 |
LA9 | 3.26 | 6188.23 | 0.0527 | 19.10 | 14974.86 | 0.1275 | 71.65 | 23929.16 | 0.2994 | 0.84 | 12303.21 | 0.0068 | 3.65 | 4847.99 | 0.0753 | 0.36 | 10895.91 | 0.0033 | 2.47 | 21371.14 | 0.0116 | 18.43 | 10548.00 | 0.1747 | 7.33 | 7698.82 | 0.0952 |
LA10 | 29.15 | 5884.38 | 0.4954 | 95.43 | 11229.43 | 0.8498 | 89.66 | 20489.06 | 0.4376 | 16.96 | 9051.65 | 0.1874 | 21.61 | 3412.09 | 0.6333 | 16.06 | 8721.17 | 0.1841 | 2.82 | 17303.92 | 0.0163 | 74.14 | 8057.43 | 0.9201 | 27.50 | 5058.68 | 0.5436 |
LA11 | 10.88 | 6259.53 | 0.1738 | 40.56 | 13131.47 | 0.3089 | 121.99 | 22020.10 | 0.5540 | 2.48 | 10555.63 | 0.0235 | 5.92 | 4847.60 | 0.1221 | 0.48 | 9784.56 | 0.0049 | 7.09 | 16593.97 | 0.0427 | 26.86 | 7655.07 | 0.3509 | 7.73 | 7284.80 | 0.1061 |
LA12 | 48.02 | 6403.75 | 0.7499 | 149.63 | 9735.88 | 1.5369 | 62.74 | 14671.39 | 0.4276 | 20.20 | 8380.51 | 0.2410 | 56.05 | 3384.94 | 1.6559 | 5.41 | 6896.77 | 0.0784 | 91.38 | 11321.11 | 0.8072 | 109.18 | 5802.35 | 1.8817 | 46.13 | 7209.85 | 0.6398 |
LA13 | 22.08 | 5983.25 | 0.3690 | 77.34 | 12359.55 | 0.6258 | 49.89 | 24426.48 | 0.2042 | 13.64 | 10650.97 | 0.1281 | 31.43 | 4286.46 | 0.7332 | 3.68 | 8877.22 | 0.0415 | 184.36 | 14885.17 | 1.2385 | 77.19 | 10677.05 | 0.7230 | 23.24 | 10012.96 | 0.2321 |
LA14 | 29.06 | 6197.60 | 0.4689 | 219.41 | 12097.18 | 1.8137 | 68.78 | 22967.64 | 0.2995 | 7.41 | 10651.10 | 0.0696 | 33.00 | 4079.19 | 0.8090 | 10.50 | 8376.22 | 0.1254 | 21.77 | 16404.09 | 0.1327 | 122.70 | 8502.69 | 1.4431 | 92.02 | 9152.03 | 1.0055 |
LA15 | 28.90 | 6409.17 | 0.4509 | 11.61 | 12035.67 | 0.0965 | 43.15 | 25371.86 | 0.1701 | 5.48 | 11118.01 | 0.0493 | 35.81 | 3597.27 | 0.9955 | 6.72 | 6464.97 | 0.1039 | 159.51 | 12763.92 | 1.2497 | 120.12 | 7008.78 | 1.7139 | 55.67 | 5676.13 | 0.9808 |
LA16 | 20.91 | 6243.38 | 0.3349 | 95.37 | 11719.51 | 0.8138 | 41.47 | 21759.15 | 0.1906 | 2.48 | 11669.90 | 0.0213 | 27.93 | 5249.47 | 0.5321 | 5.48 | 10752.39 | 0.0510 | 113.55 | 14828.06 | 0.7658 | 123.52 | 9586.25 | 1.2885 | 34.37 | 10915.62 | 0.3149 |
LA17 | 33.89 | 6029.51 | 0.5621 | 12.57 | 12627.27 | 0.0995 | 65.10 | 23746.45 | 0.2741 | 13.69 | 10538.29 | 0.1299 | 34.97 | 4468.75 | 0.7825 | 7.61 | 9975.36 | 0.0763 | 117.69 | 13313.53 | 0.8840 | 122.84 | 9248.08 | 1.3283 | 36.44 | 8620.93 | 0.4227 |
LA18 | 29.56 | 6046.27 | 0.4889 | 137.19 | 10245.05 | 1.3391 | 54.86 | 18325.38 | 0.2994 | 10.74 | 8928.25 | 0.1203 | 48.27 | 3846.06 | 1.2551 | 6.44 | 7467.00 | 0.0862 | 91.91 | 12882.36 | 0.7135 | 125.43 | 7366.73 | 1.7027 | 36.87 | 4820.33 | 0.7649 |
Sample | Val | Cit | GABA | Cys2 | Pro | Trp | Leu | Arg | Cys | ||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Free | Total | Ratio | Free | Total | Ratio | Free | Total | Ratio | Free | Total | Ratio | Free | Total | Ratio | Free | Total | Ratio | Free | Total | Ratio | Free | Total | Ratio | Free | Total | Ratio | |
SP1 | 4.42 | 6012.65 | 0.0735 | 2.49 | 4673.88 | 0.0533 | 3.46 | 3353.11 | 0.1032 | — | 11525.08 | — | 4.15 | 6607.74 | 0.0628 | 0.24 | 3637.74 | 0.0066 | 1.37 | 12211.14 | 0.0112 | 34.88 | 9489.73 | 0.3676 | — | 5538.05 | — |
SP2 | 15.20 | 10648.71 | 0.1427 | 6.07 | 3955.21 | 0.1535 | 21.27 | 4028.12 | 0.5280 | — | 13123.45 | — | 12.90 | 6341.28 | 0.2034 | 0.51 | 6868.79 | 0.0074 | 4.08 | 11230.44 | 0.0363 | 87.38 | 8979.88 | 0.9731 | — | 8675.28 | — |
SP3 | 15.36 | 6327.29 | 0.2428 | 24.59 | 4218.39 | 0.5829 | 83.54 | 2906.04 | 2.8747 | 0.67 | 9520.68 | 0.0070 | 27.55 | 7911.24 | 0.3482 | 2.67 | 6256.52 | 0.0427 | 33.86 | 9531.17 | 0.3553 | 68.39 | 8667.37 | 0.7891 | — | 3752.42 | — |
SP4 | 16.09 | 10136.97 | 0.1587 | 9.45 | 4525.17 | 0.2088 | 112.07 | 1860.38 | 6.0240 | 0.71 | 8713.26 | 0.0081 | 22.64 | 9816.87 | 0.2306 | 3.14 | 6886.81 | 0.0456 | 25.37 | 11900.95 | 0.2132 | 41.42 | 7830.13 | 0.5290 | — | 5020.00 | — |
SP5 | 12.81 | 12270.45 | 0.1044 | 3.68 | 5960.38 | 0.0617 | 9.31 | 2163.00 | 0.4304 | — | 11194.54 | — | 5.92 | 8973.82 | 0.0660 | 0.39 | 6741.47 | 0.0058 | 3.03 | 17710.97 | 0.0171 | 75.96 | 11624.78 | 0.6534 | — | 8152.58 | — |
SP6 | 18.63 | 6921.90 | 0.2691 | 54.08 | 6475.87 | 0.8351 | 110.08 | 2010.87 | 5.4742 | 0.26 | 9803.15 | 0.0027 | 28.19 | 7126.12 | 0.3956 | 4.73 | 3570.11 | 0.1325 | 26.75 | 15586.82 | 0.1716 | 43.86 | 5689.69 | 0.7709 | — | 3789.97 | — |
SP7 | 17.07 | 9259.39 | 0.1844 | 29.75 | 4140.35 | 0.7185 | 103.95 | 2825.91 | 3.6785 | 2.41 | 9778.44 | 0.0246 | 24.62 | 8226.72 | 0.2993 | 3.95 | 4856.66 | 0.0813 | 27.66 | 10861.08 | 0.2547 | 34.40 | 4988.56 | 0.6896 | — | 3471.13 | — |
SP8 | 15.86 | 7296.00 | 0.2174 | 3.13 | 4702.29 | 0.0666 | 20.48 | 1682.03 | 1.2176 | — | 6139.57 | — | 10.52 | 6680.26 | 0.1575 | 0.56 | 4883.67 | 0.0115 | 4.38 | 9640.58 | 0.0454 | 64.28 | 4886.46 | 1.3155 | — | 3120.50 | — |
SP9 | 37.20 | 6514.46 | 0.5710 | 10.18 | 5254.55 | 0.1937 | 57.02 | 2501.97 | 2.2790 | 0.14 | 10056.41 | 0.0014 | 15.77 | 5340.61 | 0.2953 | 1.20 | 4253.18 | 0.0282 | 14.42 | 11713.99 | 0.1231 | 124.64 | 5090.33 | 2.4486 | — | 3897.56 | — |
SP10 | 38.29 | 9908.44 | 0.3864 | 13.50 | 7105.23 | 0.1900 | 57.85 | 1953.05 | 2.9620 | 0.26 | 10687.68 | 0.0024 | 19.03 | 7381.05 | 0.2578 | 1.17 | 7036.56 | 0.0166 | 13.77 | 14235.69 | 0.0967 | 108.51 | 4268.65 | 2.5420 | — | 8653.16 | — |
SP11 | 21.90 | 10089.65 | 0.2171 | 8.30 | 5747.51 | 0.1444 | 108.20 | 3155.71 | 3.4287 | 0.79 | 9550.72 | 0.0083 | 24.66 | 5804.81 | 0.4248 | 1.94 | 7077.68 | 0.0274 | 26.89 | 14118.06 | 0.1905 | 67.72 | 5222.67 | 1.2967 | — | 6165.20 | — |
SP12 | 36.91 | 5104.20 | 0.7231 | 31.74 | 3267.50 | 0.9714 | 56.19 | 3092.80 | 1.8168 | 0.58 | 8821.94 | 0.0066 | 11.01 | 6511.44 | 0.1691 | 2.35 | 4185.92 | 0.0561 | 10.46 | 8633.77 | 0.1212 | 99.42 | 6433.17 | 1.5454 | — | 2940.84 | — |
SP13 | 27.76 | 5423.78 | 0.5118 | 27.14 | 3816.49 | 0.7111 | 82.12 | 2484.15 | 3.3058 | 0.99 | 6828.14 | 0.0145 | 22.06 | 7524.55 | 0.2932 | 11.42 | 3149.92 | 0.3625 | 31.25 | 8945.92 | 0.3493 | 73.82 | 5010.51 | 1.4733 | — | 6503.52 | — |
SP14 | 44.12 | 10896.32 | 0.4049 | 11.57 | 6328.11 | 0.1828 | 70.23 | 4220.23 | 1.6641 | 0.28 | 9165.62 | 0.0031 | 21.63 | 8005.88 | 0.2702 | 2.44 | 4732.57 | 0.0516 | 18.29 | 13035.53 | 0.1403 | 65.62 | 6826.41 | 0.9613 | — | 8677.59 | — |
SP15 | 6.17 | 7444.35 | 0.0829 | 5.45 | 3756.96 | 0.1451 | 4.23 | 3324.82 | 0.1272 | — | 10198.26 | — | 14.42 | 5416.47 | 0.2662 | — | 5143.26 | — | 1.80 | 14814.40 | 0.0122 | 30.19 | 5059.02 | 0.5968 | — | 6694.08 | — |
SP16 | 26.04 | 6396.60 | 0.4071 | 36.82 | 3762.26 | 0.9787 | 134.46 | 3870.97 | 3.4735 | 0.72 | 8704.16 | 0.0083 | 27.10 | 7353.81 | 0.3685 | 15.39 | 7545.48 | 0.2040 | 30.79 | 12588.03 | 0.2446 | 43.32 | 6483.46 | 0.6682 | — | 4369.57 | — |
SP17 | 9.24 | 8969.61 | 0.1030 | 4.12 | 3703.39 | 0.1112 | 2.34 | 3148.92 | 0.0743 | — | 8001.88 | — | 14.78 | 8403.61 | 0.1759 | — | 4014.56 | — | 5.35 | 10274.36 | 0.0521 | 9.96 | 4876.50 | 0.2042 | — | 6360.07 | — |
SP18 | 26.19 | 12386.71 | 0.2114 | 26.35 | 5209.87 | 0.5058 | 98.88 | 3534.87 | 2.7973 | 0.12 | 12402.18 | 0.0010 | 17.21 | 9211.57 | 0.1868 | 2.08 | 7798.78 | 0.0267 | 22.77 | 17831.20 | 0.1277 | 108.35 | 4662.91 | 2.3237 | — | 7665.04 | — |
SP19 | 25.16 | 8312.52 | 0.3027 | 8.51 | 4849.89 | 0.1755 | 73.42 | 1868.93 | 3.9285 | 0.28 | 8968.53 | 0.0031 | 17.64 | 8754.89 | 0.2015 | 3.68 | 5290.70 | 0.0696 | 16.70 | 11607.35 | 0.1439 | 80.47 | 7406.94 | 1.0864 | — | 7063.71 | — |
SP20 | 25.85 | 8395.03 | 0.3079 | 17.22 | 4586.93 | 0.3754 | 131.28 | 2873.62 | 4.5685 | 0.40 | 8894.24 | 0.0045 | 18.50 | 4472.47 | 0.4136 | 9.86 | 3888.62 | 0.2536 | 24.40 | 8829.37 | 0.2764 | 42.54 | 4662.51 | 0.9124 | — | 4657.46 | — |
SP21 | 11.27 | 5982.04 | 0.1884 | 24.90 | 4366.50 | 0.5703 | 10.82 | 3551.90 | 0.3046 | 0.24 | 8933.72 | 0.0027 | 28.86 | 6002.68 | 0.4808 | 0.15 | 6460.55 | 0.0023 | 3.89 | 12300.36 | 0.0316 | 55.98 | 5963.67 | 0.9387 | — | 5955.22 | — |
SP22 | 10.06 | 9469.47 | 0.1062 | 5.09 | 5545.62 | 0.0918 | 1.90 | 1902.37 | 0.0999 | — | 10023.81 | — | 18.22 | 5611.46 | 0.3247 | 0.14 | 3261.78 | 0.0043 | 9.76 | 12591.58 | 0.0775 | 18.52 | 8217.10 | 0.2254 | — | 6884.34 | — |
SP23 | 19.80 | 6941.68 | 0.2852 | 35.46 | 4560.09 | 0.7776 | 10.96 | 2519.87 | 0.4349 | 0.31 | 11078.52 | 0.0028 | 25.31 | 7474.12 | 0.3386 | 0.55 | 4776.94 | 0.0115 | 5.34 | 9323.61 | 0.0573 | 75.70 | 8379.33 | 0.9034 | — | 4296.33 | — |
SP24 | 24.68 | 7991.34 | 0.3088 | 29.19 | 6319.28 | 0.4619 | 4.94 | 3668.10 | 0.1347 | 0.09 | 10178.72 | 0.0009 | 23.29 | 5293.61 | 0.4400 | 0.60 | 4551.39 | 0.0132 | 3.83 | 14205.93 | 0.0270 | 58.04 | 6170.55 | 0.9406 | — | 5644.77 | — |
SP25 | 28.45 | 7459.65 | 0.3814 | 46.15 | 5088.78 | 0.9069 | 163.48 | 3897.18 | 4.1948 | 1.16 | 11215.85 | 0.0103 | 24.67 | 7584.57 | 0.3253 | 7.63 | 7080.31 | 0.1078 | 35.40 | 14162.74 | 0.2500 | 74.34 | 9656.72 | 0.7698 | — | 6166.09 | — |
LP1 | 21.36 | 7244.74 | 0.2948 | 5.16 | 4405.39 | 0.1171 | 58.37 | 2407.83 | 2.4242 | 0.13 | 13065.90 | 0.0010 | 11.76 | 8651.28 | 0.1359 | 3.62 | 5123.83 | 0.0707 | 13.93 | 10764.87 | 0.1294 | 30.24 | 8884.37 | 0.3404 | — | 4324.94 | — |
LP2 | 14.46 | 7373.76 | 0.1961 | 16.36 | 4874.65 | 0.3356 | 40.10 | 2138.03 | 1.8756 | 0.77 | 7988.91 | 0.0096 | 14.86 | 8851.40 | 0.1679 | 14.60 | 3817.53 | 0.3824 | 15.17 | 13357.73 | 0.1136 | 26.71 | 9701.11 | 0.2753 | — | 7977.29 | — |
LP3 | 9.83 | 8734.76 | 0.1125 | 60.38 | 3744.80 | 1.6124 | 14.14 | 2099.63 | 0.6735 | — | 9725.45 | — | 18.43 | 4540.45 | 0.4059 | 0.45 | 4728.65 | 0.0095 | 3.52 | 9822.04 | 0.0358 | 74.16 | 5810.06 | 1.2764 | — | 3530.69 | — |
LP4 | 35.80 | 9919.45 | 0.3609 | 3.79 | 3526.80 | 0.1075 | 122.04 | 2438.49 | 5.0047 | 0.37 | 9387.49 | 0.0039 | 18.34 | 8296.58 | 0.2211 | 1.14 | 5349.26 | 0.0213 | 22.21 | 9157.07 | 0.2425 | 48.84 | 8460.67 | 0.5773 | — | 6861.56 | — |
LP5 | 35.99 | 8871.65 | 0.4057 | 11.90 | 4587.62 | 0.2594 | 133.05 | 3159.85 | 4.2106 | 4.21 | 7298.47 | 0.0577 | 25.23 | 5061.04 | 0.4985 | 4.34 | 5510.02 | 0.0788 | 31.89 | 11224.14 | 0.2841 | 45.88 | 6939.12 | 0.6612 | — | 3854.06 | — |
LP6 | 24.78 | 7215.09 | 0.3434 | 9.73 | 4416.05 | 0.2203 | 91.48 | 2092.89 | 4.3710 | 2.32 | 7641.78 | 0.0304 | 14.71 | 6655.83 | 0.2210 | 5.76 | 3078.31 | 0.1871 | 13.10 | 10225.91 | 0.1281 | 77.03 | 6842.51 | 1.1258 | — | 3272.62 | — |
LP7 | 18.41 | 6396.21 | 0.2878 | 10.84 | 4275.51 | 0.2535 | 64.21 | 2197.40 | 2.9221 | 4.25 | 11232.05 | 0.0378 | 9.15 | 8097.10 | 0.1130 | 4.71 | 3289.73 | 0.1432 | 11.93 | 9991.64 | 0.1194 | 30.66 | 7921.66 | 0.3870 | 0.14 | 6607.63 | 0.0021 |
LP8 | 23.88 | 7401.70 | 0.3226 | 16.38 | 4758.26 | 0.3442 | 69.10 | 3565.33 | 1.9381 | 0.55 | 8739.92 | 0.0063 | 14.73 | 8982.82 | 0.1640 | 2.82 | 4541.13 | 0.0621 | 24.29 | 16149.82 | 0.1504 | 31.42 | 6643.33 | 0.4730 | — | 5673.14 | — |
LP9 | 22.74 | 8090.74 | 0.2811 | 32.17 | 6784.16 | 0.4742 | 95.67 | 3874.88 | 2.4690 | 0.34 | 11756.40 | 0.0029 | 25.48 | 9895.35 | 0.2575 | 3.59 | 4755.18 | 0.0755 | 30.55 | 11213.15 | 0.2724 | 118.53 | 7876.17 | 1.5049 | 0.22 | 4743.85 | 0.0046 |
LP10 | 44.70 | 10435.87 | 0.4283 | 15.47 | 3862.27 | 0.4005 | 82.77 | 2085.51 | 3.9688 | 0.95 | 13573.29 | 0.0070 | 21.56 | 8888.77 | 0.2426 | 0.77 | 7378.31 | 0.0104 | 16.31 | 14411.77 | 0.1132 | 63.57 | 9539.07 | 0.6664 | 0.14 | 8866.38 | 0.0016 |
LP11 | 30.18 | 7118.23 | 0.4240 | 10.12 | 3840.01 | 0.2635 | 38.62 | 2188.18 | 1.7649 | 0.39 | 9579.85 | 0.0041 | 14.31 | 6477.73 | 0.2209 | 0.56 | 4389.22 | 0.0128 | 9.34 | 10905.75 | 0.0856 | 74.49 | 5696.30 | 1.3077 | — | 5334.87 | — |
LP12 | 10.67 | 9073.21 | 0.1176 | 9.45 | 4795.94 | 0.1970 | 6.74 | 2798.07 | 0.2409 | — | 9754.10 | — | 10.14 | 5687.18 | 0.1783 | 0.22 | 6220.07 | 0.0035 | 2.99 | 11120.12 | 0.0269 | 89.48 | 9822.46 | 0.9110 | — | 7787.85 | — |
LP13 | 24.43 | 7986.97 | 0.3059 | 10.20 | 5208.39 | 0.1958 | 105.99 | 3587.82 | 2.9542 | 1.19 | 10899.43 | 0.0109 | 25.32 | 9578.40 | 0.2643 | 2.01 | 6255.14 | 0.0321 | 24.85 | 10572.72 | 0.2350 | 76.46 | 7230.87 | 1.0574 | — | 8350.79 | — |
LP14 | 21.85 | 12895.61 | 0.1694 | 10.61 | 6247.35 | 0.1698 | 114.88 | 3385.86 | 3.3929 | 0.65 | 12941.50 | 0.0050 | 24.21 | 10165.28 | 0.2382 | 0.42 | 5801.66 | 0.0072 | 24.35 | 12767.46 | 0.1907 | 38.76 | 9267.96 | 0.4182 | 0.10 | 4749.52 | 0.0021 |
LP15 | 30.04 | 10600.31 | 0.2834 | 8.02 | 6112.10 | 0.1312 | 39.61 | 2063.22 | 1.9198 | 0.26 | 10874.29 | 0.0024 | 14.42 | 6109.32 | 0.2360 | 0.63 | 7137.55 | 0.0088 | 7.68 | 12394.41 | 0.0620 | 71.29 | 6514.00 | 1.0944 | — | 4351.01 | — |
LP16 | 26.39 | 5728.98 | 0.4606 | 10.83 | 4203.58 | 0.2576 | 96.25 | 3176.63 | 3.0299 | 3.59 | 6782.15 | 0.0529 | 16.36 | 8733.17 | 0.1873 | 4.94 | 6147.21 | 0.0804 | 18.97 | 9376.15 | 0.2023 | 51.19 | 8498.90 | 0.6023 | 0.14 | 5213.83 | 0.0027 |
LA1 | 45.00 | 5702.67 | 0.7891 | 13.87 | 3437.80 | 0.4035 | 97.36 | 3259.71 | 2.9868 | 0.34 | 9934.14 | 0.0034 | 16.88 | 5375.18 | 0.3140 | 5.39 | 5649.12 | 0.0954 | 25.80 | 11813.05 | 0.2184 | 46.47 | 7148.37 | 0.6501 | — | 6609.48 | — |
LA2 | 19.62 | 10334.80 | 0.1898 | 28.58 | 3747.09 | 0.7627 | 81.64 | 2390.45 | 3.4153 | 0.86 | 7845.80 | 0.0110 | 12.38 | 8095.98 | 0.1529 | 2.57 | 5265.52 | 0.0488 | 23.74 | 15177.45 | 0.1564 | 33.03 | 7150.11 | 0.4620 | — | 5064.86 | — |
LA3 | 39.08 | 11531.59 | 0.3389 | 11.17 | 7658.73 | 0.1458 | 96.78 | 3420.33 | 2.8296 | 0.34 | 9648.03 | 0.0035 | 25.22 | 6740.71 | 0.3741 | 14.39 | 6990.99 | 0.2058 | 28.29 | 16947.54 | 0.1669 | 35.87 | 11157.91 | 0.3215 | 0.12 | 5317.49 | 0.0023 |
LA4 | 30.49 | 9094.55 | 0.3353 | 5.75 | 3337.54 | 0.1723 | 59.10 | 1960.88 | 3.0140 | — | 7030.26 | — | 21.76 | 7834.00 | 0.2778 | 1.38 | 6225.95 | 0.0222 | 12.30 | 8279.16 | 0.1486 | 67.99 | 6933.19 | 0.9806 | — | 5458.32 | — |
LA5 | 41.41 | 10636.04 | 0.3893 | 53.62 | 5018.49 | 1.0684 | 47.11 | 3686.56 | 1.2779 | 1.53 | 7468.47 | 0.0205 | 28.74 | 6068.81 | 0.4736 | 1.29 | 4893.15 | 0.0264 | 22.91 | 11517.82 | 0.1989 | 104.70 | 5679.74 | 1.8434 | — | 6326.99 | — |
LA6 | 26.86 | 10152.01 | 0.2646 | 10.02 | 5879.06 | 0.1704 | 109.90 | 3477.43 | 3.1604 | 0.66 | 10000.06 | 0.0066 | 23.06 | 5135.21 | 0.4491 | 7.12 | 3736.03 | 0.1906 | 33.26 | 15229.07 | 0.2184 | 38.54 | 6481.81 | 0.5946 | 0.24 | 5289.57 | 0.0045 |
LA7 | 37.39 | 5299.21 | 0.7056 | 7.21 | 4582.47 | 0.1573 | 137.64 | 3164.85 | 4.3490 | — | 6820.33 | — | 25.78 | 4508.55 | 0.5718 | 2.81 | 5062.03 | 0.0555 | 30.16 | 14035.50 | 0.2149 | 37.11 | 8557.89 | 0.4336 | — | 6719.24 | — |
LA8 | 33.07 | 10470.63 | 0.3158 | 14.37 | 6157.76 | 0.2334 | 26.46 | 2514.35 | 1.0524 | — | 12359.55 | — | 27.27 | 8147.42 | 0.3347 | 3.27 | 7846.61 | 0.0417 | 18.76 | 12842.68 | 0.1461 | 88.93 | 8060.89 | 1.1032 | — | 4516.77 | — |
LA9 | 3.40 | 12345.60 | 0.0275 | 9.98 | 5675.92 | 0.1758 | 2.04 | 4742.21 | 0.0430 | — | 15127.20 | — | 6.76 | 10585.00 | 0.0639 | 0.29 | 8961.94 | 0.0032 | 1.34 | 12900.12 | 0.0104 | 13.42 | 10716.38 | 0.1252 | — | 5820.67 | — |
LA10 | 32.37 | 8506.23 | 0.3805 | 15.12 | 4869.46 | 0.3105 | 40.05 | 3515.47 | 1.1393 | 0.11 | 11225.08 | 0.0010 | 15.86 | 4962.73 | 0.3196 | 1.40 | 6261.69 | 0.0224 | 8.88 | 9143.75 | 0.0971 | 86.86 | 5400.65 | 1.6083 | — | 7815.34 | — |
LA11 | 5.06 | 11187.35 | 0.0452 | 16.69 | 6130.94 | 0.2722 | 2.35 | 2533.92 | 0.0927 | 0.36 | 13012.18 | 0.0028 | 5.43 | 10083.36 | 0.0539 | 0.97 | 5607.42 | 0.0173 | 1.87 | 15543.78 | 0.0120 | 7.40 | 7751.63 | 0.0955 | — | 4413.68 | — |
LA12 | 38.36 | 6567.71 | 0.5841 | 37.15 | 4331.21 | 0.8577 | 73.93 | 1638.83 | 4.5111 | 0.13 | 10001.78 | 0.0013 | 19.64 | 5219.17 | 0.3763 | 1.98 | 3334.61 | 0.0594 | 16.66 | 13924.60 | 0.1196 | 43.90 | 4477.46 | 0.9805 | — | 3708.31 | — |
LA13 | 33.49 | 9334.49 | 0.3588 | 21.25 | 5427.96 | 0.3915 | 57.89 | 3900.56 | 1.4841 | 0.15 | 11927.09 | 0.0013 | 18.94 | 7570.98 | 0.2502 | 1.52 | 3832.52 | 0.0397 | 14.66 | 14801.24 | 0.0990 | 130.27 | 9104.06 | 1.4309 | — | 4437.40 | — |
LA14 | 48.22 | 9862.78 | 0.4889 | 10.25 | 5078.48 | 0.2018 | 68.84 | 1820.89 | 3.7806 | 0.18 | 12167.89 | 0.0015 | 20.49 | 9331.57 | 0.2196 | 3.12 | 6487.16 | 0.0481 | 27.88 | 15343.15 | 0.1817 | 73.66 | 5754.34 | 1.2801 | — | 4845.05 | — |
LA15 | 33.62 | 4870.39 | 0.6903 | 38.52 | 4318.57 | 0.8920 | 46.08 | 2538.52 | 1.8152 | — | 8893.47 | — | 18.06 | 5895.62 | 0.3063 | 0.58 | 3366.22 | 0.0172 | 18.67 | 7744.33 | 0.2411 | 97.99 | 6807.78 | 1.4394 | 0.19 | 6361.27 | 0.0030 |
LA16 | 28.73 | 10676.60 | 0.2691 | 17.16 | 5956.75 | 0.2881 | 30.34 | 4234.41 | 0.7165 | 0.10 | 10936.85 | 0.0009 | 20.90 | 7579.89 | 0.2757 | 1.06 | 6621.76 | 0.0160 | 17.03 | 15351.32 | 0.1109 | 39.65 | 7199.84 | 0.5507 | 0.16 | 4666.63 | 0.0034 |
LA17 | 25.33 | 11534.16 | 0.2196 | 8.43 | 5093.69 | 0.1655 | 57.74 | 3692.35 | 1.5638 | 0.41 | 10297.60 | 0.0040 | 12.50 | 7491.18 | 0.1669 | 4.43 | 3719.53 | 0.1191 | 16.02 | 17696.48 | 0.0905 | 32.56 | 9782.67 | 0.3328 | — | 8946.44 | — |
LA18 | 24.44 | 9269.41 | 0.2637 | 7.67 | 3801.25 | 0.2018 | 56.82 | 3179.11 | 1.7873 | 0.60 | 9490.75 | 0.0063 | 15.74 | 6342.64 | 0.2482 | 2.92 | 3811.54 | 0.0766 | 15.43 | 11215.99 | 0.1376 | 37.22 | 8358.76 | 0.4453 | — | 3946.18 | — |
Sample | Hpro | Met | Thr | Asn | Gln | Ser | Total free amino acids | Total proteins (%) | |||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Free | Total | Ratio | Free | Total | Ratio | Free | Total | Ratio | Free | Total | Ratio | Free | Total | Ratio | Free | Total | Ratio | ||||||||||
SP1 | 0.34 | 12275.50 | 0.0028 | — | 2102.26 | — | 12.78 | 7963.72 | 0.1605 | 39.82 | 3834.80 | 1.0384 | 1.04 | 1971.45 | 0.0528 | 4.76 | 6466.09 | 0.0736 | 172.07 | 16.19 | |||||||
SP2 | 0.84 | 11585.53 | 0.0073 | — | 1927.32 | — | 38.01 | 7239.98 | 0.5250 | 215.37 | 4986.79 | 4.3188 | 3.20 | 2096.01 | 0.1527 | 22.43 | 5929.40 | 0.3783 | 646.85 | 18.88 | |||||||
SP3 | 10.37 | 9975.53 | 0.1040 | 4.20 | 1384.33 | 0.3034 | 157.92 | 4945.98 | 3.1929 | 133.70 | 3711.29 | 3.6025 | 68.98 | 1951.23 | 3.5352 | 144.02 | 5954.58 | 2.4186 | 1437.51 | 15.19 | |||||||
SP4 | 3.50 | 12741.33 | 0.0275 | — | 1958.62 | — | 168.24 | 8709.13 | 1.9318 | 139.13 | 4253.91 | 3.2706 | 143.70 | 2036.62 | 7.0558 | 163.42 | 5609.49 | 2.9133 | 1520.16 | 17.82 | |||||||
SP5 | 0.48 | 15829.31 | 0.0030 | — | 2151.19 | — | 34.91 | 8355.57 | 0.4178 | 258.84 | 4912.45 | 5.2691 | 3.06 | 2256.83 | 0.1356 | 16.69 | 10234.90 | 0.1631 | 546.01 | 20.46 | |||||||
SP6 | 6.53 | 12371.35 | 0.0528 | — | 1608.64 | — | 185.93 | 5726.31 | 3.2469 | 131.14 | 4602.59 | 2.8493 | 53.20 | 2164.53 | 2.4578 | 144.62 | 8041.81 | 1.7984 | 1568.88 | 17.51 | |||||||
SP7 | 3.37 | 12423.61 | 0.0271 | 0.10 | 1470.47 | 0.0068 | 168.48 | 4848.42 | 3.4749 | 137.82 | 3978.83 | 3.4638 | 115.64 | 2145.57 | 5.3897 | 153.92 | 8601.82 | 1.7894 | 1616.80 | 15.59 | |||||||
SP8 | 0.83 | 9760.19 | 0.0085 | — | 1320.98 | — | 36.00 | 5251.98 | 0.6855 | 243.18 | 3721.89 | 6.5338 | 4.33 | 2074.31 | 0.2087 | 23.02 | 7285.14 | 0.3160 | 601.76 | 14.41 | |||||||
SP9 | 2.33 | 10641.34 | 0.0219 | 0.10 | 1927.64 | 0.0052 | 101.08 | 6224.18 | 1.6240 | 287.73 | 3643.59 | 7.8969 | 22.11 | 2123.14 | 1.0414 | 80.72 | 7300.64 | 1.1057 | 1176.53 | 14.43 | |||||||
SP10 | 5.44 | 15070.02 | 0.0361 | 0.26 | 2233.47 | 0.0116 | 111.26 | 9030.91 | 1.2320 | 295.67 | 4158.01 | 7.1109 | 19.66 | 2820.23 | 0.6971 | 98.55 | 9877.31 | 0.9977 | 1162.05 | 19.14 | |||||||
SP11 | 11.23 | 13641.99 | 0.0823 | 0.28 | 1744.92 | 0.0160 | 176.59 | 7985.90 | 2.2113 | 140.18 | 4655.28 | 3.0112 | 144.55 | 2112.77 | 6.8417 | 179.12 | 6088.37 | 2.9420 | 1570.89 | 17.01 | |||||||
SP12 | 12.01 | 11162.90 | 0.1076 | 0.24 | 1881.32 | 0.0128 | 125.79 | 4403.32 | 2.8567 | 137.25 | 3369.96 | 4.0727 | 41.76 | 1600.68 | 2.6089 | 57.70 | 6605.01 | 0.8736 | 1145.82 | 14.13 | |||||||
SP13 | 2.39 | 10615.66 | 0.0225 | 3.51 | 1382.26 | 0.2539 | 228.15 | 6391.62 | 3.5695 | 165.25 | 4095.51 | 4.0349 | 269.51 | 1696.44 | 15.8868 | 168.80 | 6708.25 | 2.5163 | 1755.14 | 13.75 | |||||||
SP14 | 1.44 | 13205.06 | 0.0109 | — | 2393.23 | — | 132.66 | 8976.95 | 1.4778 | 149.56 | 4798.05 | 3.1171 | 125.79 | 2465.90 | 5.1012 | 129.14 | 7298.41 | 1.7694 | 1255.50 | 19.06 | |||||||
SP15 | 6.10 | 10366.51 | 0.0588 | — | 1814.22 | — | 15.94 | 7685.12 | 0.2074 | 22.02 | 4676.65 | 0.4708 | 6.45 | 2382.45 | 0.2707 | 7.22 | 5548.49 | 0.1301 | 194.11 | 15.85 | |||||||
SP16 | 7.67 | 12903.86 | 0.0594 | 0.23 | 2509.21 | 0.0092 | 85.06 | 5550.87 | 1.5324 | 142.06 | 4329.68 | 3.2811 | 176.62 | 3056.34 | 5.7788 | 145.74 | 5866.53 | 2.4843 | 1678.57 | 18.29 | |||||||
SP17 | 2.03 | 10626.57 | 0.0191 | — | 1794.68 | — | 10.28 | 5329.55 | 0.1929 | 3.24 | 3731.24 | 0.0868 | 2.10 | 2828.19 | 0.0743 | 6.37 | 4657.92 | 0.1368 | 166.05 | 15.26 | |||||||
SP18 | 3.88 | 12874.45 | 0.0301 | 0.25 | 2623.84 | 0.0095 | 124.83 | 7649.77 | 1.6318 | 148.44 | 4745.86 | 3.1278 | 123.11 | 3038.81 | 4.0513 | 102.24 | 8464.54 | 1.2079 | 1304.51 | 19.91 | |||||||
SP19 | 6.64 | 13923.42 | 0.0477 | 0.39 | 1611.21 | 0.0242 | 116.76 | 6748.38 | 1.7302 | 49.92 | 4539.22 | 1.0997 | 45.19 | 3232.29 | 1.3981 | 77.95 | 5525.06 | 1.4108 | 1063.99 | 17.50 | |||||||
SP20 | 1.26 | 8623.89 | 0.0146 | 0.17 | 1655.87 | 0.0103 | 184.54 | 4751.82 | 3.8836 | 152.52 | 3938.78 | 3.8723 | 120.92 | 2150.51 | 5.6229 | 176.26 | 7450.83 | 2.3656 | 1670.95 | 13.61 | |||||||
SP21 | 10.27 | 10862.57 | 0.0945 | — | 1789.85 | — | 19.55 | 7093.47 | 0.2756 | 38.03 | 4126.30 | 0.9216 | 7.72 | 2908.79 | 0.2654 | 11.94 | 6041.00 | 0.1976 | 460.11 | 16.55 | |||||||
SP22 | 3.58 | 11476.96 | 0.0312 | 0.36 | 1441.11 | 0.0250 | 11.15 | 5896.68 | 0.1891 | 3.63 | 3801.35 | 0.0955 | 8.88 | 2075.29 | 0.4279 | 6.66 | 7009.63 | 0.0950 | 192.55 | 15.30 | |||||||
SP23 | 6.80 | 11931.55 | 0.0570 | — | 1557.75 | — | 48.22 | 5634.77 | 0.8558 | 90.90 | 3869.18 | 2.3493 | 14.04 | 1927.16 | 0.7285 | 18.66 | 8290.65 | 0.2251 | 651.61 | 16.73 | |||||||
SP24 | 6.27 | 10593.16 | 0.0592 | 0.12 | 2127.69 | 0.0056 | 64.57 | 5101.21 | 1.2658 | 104.24 | 3837.96 | 2.7160 | 14.64 | 2823.06 | 0.5186 | 24.13 | 9012.82 | 0.2677 | 734.27 | 16.87 | |||||||
SP25 | 1.86 | 13824.44 | 0.0135 | 0.44 | 2716.54 | 0.0162 | 186.66 | 4842.06 | 3.8550 | 146.65 | 4187.28 | 3.5023 | 97.55 | 2771.87 | 3.5193 | 189.30 | 8595.86 | 2.2022 | 1899.50 | 19.76 | |||||||
LP1 | 0.47 | 14631.38 | 0.0032 | 1.18 | 1948.75 | 0.0606 | 65.83 | 6231.55 | 1.0564 | 95.95 | 4013.49 | 2.3907 | 86.11 | 3138.12 | 2.7440 | 85.73 | 5753.40 | 1.4901 | 814.99 | 18.47 | |||||||
LP2 | 0.64 | 12206.18 | 0.0052 | 1.85 | 2297.38 | 0.0805 | 78.50 | 5838.37 | 1.3446 | 145.43 | 4899.76 | 2.9681 | 52.18 | 2104.26 | 2.4797 | 73.92 | 6212.65 | 1.1898 | 1012.65 | 17.73 | |||||||
LP3 | 4.52 | 8885.71 | 0.0509 | 0.13 | 1858.23 | 0.0070 | 41.09 | 5789.60 | 0.7097 | 124.04 | 2882.55 | 4.3031 | 10.24 | 2456.95 | 0.4168 | 10.18 | 6286.97 | 0.1619 | 597.23 | 13.95 | |||||||
LP4 | 5.45 | 10112.04 | 0.0539 | — | 1630.58 | — | 163.52 | 7525.00 | 2.1730 | 143.69 | 3758.46 | 3.8231 | 51.80 | 1875.09 | 2.7625 | 160.80 | 7731.94 | 2.0797 | 1426.96 | 16.05 | |||||||
LP5 | 1.71 | 9028.04 | 0.0189 | — | 1621.37 | — | 152.81 | 4615.56 | 3.3108 | 134.30 | 3260.74 | 4.1187 | 112.30 | 1704.64 | 6.5879 | 122.16 | 4651.12 | 2.6265 | 1718.87 | 15.03 | |||||||
LP6 | 0.71 | 11053.96 | 0.0064 | — | 1379.86 | — | 85.21 | 6409.94 | 1.3293 | 270.66 | 3875.46 | 6.9839 | 136.75 | 1528.04 | 8.9494 | 72.78 | 6453.61 | 1.1277 | 1308.25 | 13.94 | |||||||
LP7 | 0.41 | 10437.44 | 0.0039 | 0.99 | 1616.03 | 0.0613 | 48.37 | 6713.60 | 0.7205 | 199.42 | 3616.48 | 5.5142 | 71.18 | 2426.57 | 2.9334 | 73.61 | 5273.31 | 1.3959 | 1113.74 | 16.11 | |||||||
LP8 | 0.60 | 12028.82 | 0.0050 | 2.77 | 2130.37 | 0.1300 | 64.52 | 8264.66 | 0.7807 | 93.71 | 4102.87 | 2.2840 | 128.62 | 3030.50 | 4.2442 | 77.21 | 8764.09 | 0.8810 | 1028.91 | 17.52 | |||||||
LP9 | 2.85 | 15395.17 | 0.0185 | 1.09 | 2611.93 | 0.0417 | 95.31 | 6079.19 | 1.5678 | 135.68 | 5800.10 | 2.3393 | 21.02 | 2847.50 | 0.7382 | 160.76 | 6714.80 | 2.3941 | 1620.64 | 20.09 | |||||||
LP10 | 3.15 | 12510.19 | 0.0252 | 0.19 | 2294.16 | 0.0083 | 139.99 | 5986.95 | 2.3383 | 316.13 | 4149.54 | 7.6184 | 20.34 | 2755.14 | 0.7383 | 126.41 | 9249.73 | 1.3666 | 1408.67 | 18.88 | |||||||
LP11 | 2.02 | 11304.83 | 0.0179 | 0.44 | 1616.02 | 0.0272 | 92.06 | 7087.95 | 1.2988 | 100.62 | 3134.85 | 3.2097 | 9.67 | 2255.64 | 0.4287 | 81.26 | 7479.28 | 1.0865 | 735.57 | 14.11 | |||||||
LP12 | 1.26 | 11500.95 | 0.0110 | 0.34 | 1721.63 | 0.0197 | 24.77 | 6419.44 | 0.3859 | 25.52 | 4463.29 | 0.5718 | 3.93 | 2645.29 | 0.1486 | 15.06 | 7598.99 | 0.1982 | 291.40 | 16.46 | |||||||
LP13 | 6.41 | 13163.18 | 0.0487 | 0.30 | 2716.29 | 0.0110 | 172.44 | 9432.07 | 1.8282 | 143.41 | 4225.29 | 3.3941 | 134.72 | 2932.22 | 4.5945 | 169.75 | 8497.59 | 1.9976 | 1667.79 | 19.79 | |||||||
LP14 | 3.64 | 16225.57 | 0.0224 | 0.17 | 2793.92 | 0.0061 | 175.47 | 6457.65 | 2.7172 | 140.65 | 5052.79 | 2.7836 | 118.28 | 3874.56 | 3.0527 | 171.21 | 9352.55 | 1.8306 | 1529.56 | 21.15 | |||||||
LP15 | 1.70 | 11614.17 | 0.0146 | 0.09 | 2501.01 | 0.0036 | 89.92 | 6615.18 | 1.3593 | 269.56 | 3725.94 | 7.2347 | 3.34 | 2877.91 | 0.1161 | 82.11 | 8616.92 | 0.9529 | 900.44 | 18.46 | |||||||
LP16 | 0.94 | 11631.20 | 0.0081 | 0.99 | 1651.61 | 0.0599 | 95.46 | 5189.08 | 1.8396 | 201.46 | 3964.71 | 5.0813 | 106.74 | 2083.97 | 5.1220 | 89.52 | 5649.83 | 1.5845 | 1381.04 | 15.81 | |||||||
LA1 | 0.75 | 10525.37 | 0.0071 | 0.33 | 1722.41 | 0.0192 | 119.80 | 5747.08 | 2.0845 | 146.08 | 4354.39 | 3.3548 | 117.79 | 2828.86 | 4.1639 | 99.93 | 8354.17 | 1.1962 | 1262.44 | 15.27 | |||||||
LA2 | 0.71 | 11050.46 | 0.0064 | 2.14 | 2075.76 | 0.1031 | 88.95 | 5755.14 | 1.5456 | 114.22 | 4714.20 | 2.4229 | 113.30 | 2590.48 | 4.3737 | 128.49 | 8446.54 | 1.5212 | 1057.50 | 16.42 | |||||||
LA3 | 1.58 | 14292.88 | 0.0111 | — | 2387.79 | — | 85.96 | 9803.09 | 0.8769 | 129.12 | 5651.48 | 2.2847 | 172.44 | 2107.17 | 8.1835 | 147.16 | 11408.14 | 1.2900 | 1716.54 | 20.72 | |||||||
LA4 | 0.78 | 11457.33 | 0.0068 | — | 2109.40 | — | 75.39 | 7598.12 | 0.9922 | 177.20 | 4225.71 | 4.1934 | 34.18 | 2341.13 | 1.4600 | 45.77 | 7073.90 | 0.6470 | 778.32 | 15.58 | |||||||
LA5 | 6.18 | 11098.92 | 0.0557 | 0.60 | 1527.50 | 0.0393 | 127.09 | 6426.35 | 1.9776 | 196.17 | 4798.09 | 4.0885 | 29.66 | 2500.29 | 1.1863 | 66.00 | 7750.15 | 0.8516 | 1332.28 | 16.58 | |||||||
LA6 | 1.69 | 11256.40 | 0.0150 | 0.14 | 1485.02 | 0.0094 | 92.57 | 6651.83 | 1.3916 | 129.99 | 4328.83 | 3.0029 | 86.79 | 1722.57 | 5.0384 | 157.13 | 8406.98 | 1.8690 | 1547.66 | 16.04 | |||||||
LA7 | 2.07 | 11307.16 | 0.0183 | — | 1577.93 | — | 92.43 | 4656.66 | 1.9849 | 145.34 | 4362.67 | 3.3314 | 135.71 | 1877.25 | 7.2292 | 170.21 | 8033.47 | 2.1188 | 1517.92 | 14.71 | |||||||
LA8 | 10.79 | 15918.39 | 0.0678 | 0.19 | 2259.56 | 0.0084 | 117.78 | 7883.83 | 1.4939 | 154.15 | 5792.38 | 2.6613 | 216.67 | 2621.75 | 8.2643 | 61.37 | 7941.33 | 0.7728 | 1171.00 | 21.30 | |||||||
LA9 | 0.76 | 14178.74 | 0.0054 | 0.14 | 2618.77 | 0.0053 | 6.51 | 6538.81 | 0.0996 | 7.87 | 4624.51 | 0.1702 | 2.68 | 2321.32 | 0.1155 | 6.76 | 10561.97 | 0.0640 | 189.02 | 21.46 | |||||||
LA10 | 1.44 | 11088.93 | 0.0130 | 0.26 | 1806.72 | 0.0144 | 62.25 | 6942.43 | 0.8967 | 53.97 | 4802.60 | 1.1238 | 47.46 | 2041.76 | 2.3245 | 36.17 | 5403.37 | 0.6694 | 775.54 | 16.41 | |||||||
LA11 | 0.84 | 15220.71 | 0.0055 | 1.65 | 2528.91 | 0.0652 | 8.60 | 7038.75 | 0.1222 | 1.31 | 5236.37 | 0.0250 | 4.91 | 2429.40 | 0.2021 | 7.80 | 6092.24 | 0.1280 | 289.21 | 19.05 | |||||||
LA12 | 2.55 | 9164.65 | 0.0278 | 0.18 | 1894.44 | 0.0095 | 174.89 | 4404.00 | 3.9712 | 138.27 | 3675.47 | 3.7620 | 72.48 | 1770.28 | 4.0943 | 85.28 | 5128.63 | 1.6628 | 1294.14 | 14.54 | |||||||
LA13 | 2.16 | 13906.13 | 0.0155 | — | 2514.70 | — | 116.52 | 7070.17 | 1.6481 | 294.15 | 3964.62 | 7.4194 | 42.77 | 2727.93 | 1.5679 | 65.75 | 7050.95 | 0.9325 | 1282.38 | 18.31 | |||||||
LA14 | 9.08 | 13760.74 | 0.0660 | 0.60 | 2297.56 | 0.0261 | 164.90 | 9014.10 | 1.8294 | 131.85 | 5045.39 | 2.6133 | 63.90 | 2704.59 | 2.3627 | 144.27 | 5601.90 | 2.5754 | 1371.90 | 17.81 | |||||||
LA15 | 3.69 | 8467.71 | 0.0436 | 0.30 | 1826.09 | 0.0164 | 135.18 | 5450.03 | 2.4804 | 62.94 | 3497.92 | 1.7994 | 46.02 | 1747.54 | 2.6334 | 51.56 | 4656.58 | 1.1073 | 1020.37 | 13.89 | |||||||
LA16 | 2.78 | 16217.53 | 0.0171 | 0.29 | 2603.75 | 0.0111 | 97.73 | 8455.20 | 1.1559 | 22.61 | 5055.17 | 0.4473 | 18.70 | 2206.25 | 0.8476 | 48.71 | 9026.29 | 0.5396 | 811.03 | 20.42 | |||||||
LA17 | 0.68 | 12202.21 | 0.0056 | 2.23 | 2011.22 | 0.1109 | 81.41 | 5966.49 | 1.3645 | 207.86 | 3770.32 | 5.5131 | 55.84 | 3327.23 | 1.6783 | 76.89 | 7660.33 | 1.0037 | 1027.14 | 18.44 | |||||||
LA18 | 0.55 | 9875.86 | 0.0056 | 2.42 | 1754.15 | 0.1380 | 76.82 | 5468.75 | 1.4047 | 143.75 | 3704.63 | 3.8803 | 52.67 | 1760.52 | 2.9917 | 90.61 | 6192.83 | 1.4631 | 1068.96 | 14.93 |
—, analytes were not detected.
Twenty-six minerals, total proteins, and the content of amino acids after hydrolysis are shown in Tables
Contents of 26 minerals in living water of three species of duckweed (
Sample | Ti | V | Cr | Mn | Co | Ni | Cu | Zn | As | Se | Sr | Mo | Cd | Sn | Sb | Ba | Tl | Pb |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
SP1 | 22.831 | 2.900 | 2.178 | 47.015 | 0.181 | 1.860 | 1.061 | 90.405 | 1.530 | 1.578 | 261.614 | 2.840 | 0.007 | 0.087 | 0.440 | 96.840 | 0.003 | 0.058 |
SP2 | 27.277 | 1.017 | 2.922 | 36.990 | 0.177 | 1.648 | 0.806 | 80.395 | 2.091 | 1.288 | 199.006 | 3.275 | 0.006 | 0.007 | 0.516 | 60.613 | 0.003 | 0.001 |
SP3 | 35.621 | 1.915 | 1.838 | 41.295 | 0.162 | 2.899 | 1.500 | 56.437 | 1.127 | 1.983 | 172.960 | 3.017 | 0.016 | 0.168 | 0.386 | 70.196 | 0.002 | 0.024 |
SP4 | 22.183 | 3.099 | 1.687 | 55.683 | 0.214 | 1.767 | 1.690 | 63.717 | 3.164 | 1.935 | 178.985 | 0.407 | 0.014 | 0.168 | 0.487 | 77.659 | 0.001 | 0.041 |
SP5 | 45.839 | 3.162 | 1.413 | 24.431 | 0.189 | 2.829 | 0.615 | 71.741 | 0.920 | 1.861 | 275.418 | 2.211 | 0.011 | 0.098 | 0.542 | 63.126 | 0.004 | 0.074 |
SP6 | 41.446 | 1.783 | 2.371 | 2.110 | 0.240 | 2.157 | 2.805 | 87.665 | 2.146 | 1.464 | 193.120 | 0.577 | 0.002 | 0.121 | 0.536 | 39.704 | 0.004 | 0.046 |
SP7 | 37.099 | 2.423 | 2.068 | 13.993 | 0.219 | 2.583 | 1.424 | 88.936 | 2.601 | 1.747 | 165.967 | 1.562 | 0.010 | 0.095 | 0.316 | 69.916 | 0.003 | 0.050 |
SP8 | 28.377 | 2.251 | 2.276 | 54.028 | 0.197 | 1.709 | 2.941 | 46.581 | 2.262 | 2.139 | 249.180 | 2.345 | 0.015 | 0.125 | 0.359 | 110.809 | 0.004 | 0.047 |
SP9 | 17.084 | 1.069 | 2.452 | 40.559 | 0.192 | 3.140 | 1.247 | 29.974 | 2.770 | 1.664 | 163.694 | 2.659 | 0.015 | 0.139 | 0.637 | 60.895 | 0.004 | 0.018 |
SP10 | 37.166 | 3.316 | 1.705 | 35.254 | 0.184 | 1.793 | 2.040 | 78.451 | 1.247 | 1.407 | 174.872 | 0.632 | 0.002 | 0.137 | 0.720 | 86.360 | — | 0.003 |
SP11 | 24.163 | 1.033 | 1.368 | 60.738 | 0.190 | 3.261 | 2.894 | 66.981 | 1.135 | 2.291 | 207.084 | 1.840 | 0.003 | 0.160 | 0.488 | 117.603 | 0.004 | 0.046 |
SP12 | 38.630 | 2.597 | 1.146 | 53.768 | 0.252 | 2.550 | 1.505 | 76.263 | 1.772 | 2.126 | 180.679 | 0.626 | 0.003 | 0.124 | 0.628 | 142.280 | 0.001 | 0.062 |
SP13 | 39.728 | 2.347 | 1.577 | 26.733 | 0.173 | 3.173 | 2.882 | 75.098 | 1.628 | 1.112 | 225.261 | 0.323 | 0.012 | 0.106 | 0.550 | 95.230 | 0.001 | 0.001 |
SP14 | 39.483 | 2.488 | 2.539 | 3.742 | 0.249 | 2.137 | 1.427 | 55.271 | 1.670 | 1.581 | 154.375 | 3.214 | 0.019 | 0.135 | 0.338 | 119.138 | 0.003 | 0.043 |
SP15 | 19.629 | 2.734 | 2.326 | 22.405 | 0.198 | 3.153 | 1.427 | 55.631 | 1.175 | 2.065 | 247.855 | 2.928 | 0.011 | 0.011 | 0.633 | 136.536 | 0.004 | 0.030 |
SP16 | 34.619 | 1.068 | 2.064 | 52.250 | 0.163 | 2.169 | 3.017 | 54.488 | 2.321 | 1.985 | 253.553 | 2.155 | 0.002 | 0.120 | 0.377 | 68.487 | 0.001 | 0.022 |
SP17 | 28.672 | 3.244 | 2.192 | 31.414 | 0.159 | 1.880 | 2.871 | 91.946 | 1.444 | 1.287 | 175.165 | 1.721 | 0.018 | 0.138 | 0.669 | 91.883 | 0.004 | 0.030 |
SP18 | 25.018 | 1.789 | 2.378 | 45.984 | 0.183 | 2.570 | 2.365 | 24.786 | 1.877 | 2.146 | 194.164 | 3.450 | 0.007 | 0.049 | 0.318 | 95.469 | 0.002 | 0.057 |
SP19 | 22.802 | 2.136 | 2.199 | 53.284 | 0.212 | 2.704 | 1.054 | 85.613 | 0.913 | 2.073 | 269.684 | 3.174 | 0.014 | 0.043 | 0.423 | 111.149 | 0.003 | 0.004 |
SP20 | 40.638 | 1.682 | 1.746 | 14.621 | 0.180 | 1.611 | 2.705 | 73.740 | 3.221 | 2.055 | 225.646 | 3.023 | 0.013 | 0.159 | 0.450 | 137.841 | 0.004 | 0.019 |
SP21 | 23.513 | 1.413 | 2.811 | 12.908 | 0.236 | 1.959 | 0.607 | 22.616 | 2.711 | 1.170 | 227.681 | 3.187 | 0.005 | 0.037 | 0.205 | 62.615 | 0.003 | 0.016 |
SP22 | 32.010 | 1.755 | 2.461 | 56.750 | 0.202 | 2.515 | 1.114 | 80.116 | 1.210 | 2.322 | 223.754 | 1.075 | 0.015 | 0.155 | 0.573 | 96.469 | 0.002 | 0.012 |
SP23 | 17.817 | 2.259 | 2.391 | 43.452 | 0.219 | 1.903 | 0.700 | 88.007 | 3.277 | 1.855 | 155.450 | 2.793 | 0.014 | 0.164 | 0.311 | 82.020 | 0.002 | 0.010 |
SP24 | 24.306 | 2.384 | 1.841 | 32.935 | 0.253 | 1.896 | 3.027 | 63.243 | 1.975 | 1.109 | 214.325 | 2.979 | 0.008 | 0.044 | 0.642 | 47.431 | 0.003 | 0.061 |
SP25 | 30.842 | 2.878 | 1.693 | 29.377 | 0.254 | 2.786 | 1.811 | 69.276 | 1.082 | 1.349 | 218.438 | 0.896 | 0.011 | 0.125 | 0.474 | 126.036 | 0.001 | 0.032 |
LP1 | 37.974 | 1.441 | 2.756 | 42.898 | 0.251 | 1.828 | 2.325 | 46.010 | 2.655 | 2.264 | 162.610 | 0.298 | 0.004 | 0.049 | 0.368 | 106.287 | 0.002 | 0.055 |
LP2 | 25.601 | 3.144 | 1.914 | 10.035 | 0.173 | 2.425 | 2.422 | 53.173 | 1.031 | 1.740 | 211.860 | 0.256 | 0.017 | 0.153 | 0.596 | 73.995 | 0.002 | 0.001 |
LP3 | 40.576 | 2.424 | 1.579 | 36.158 | 0.203 | 2.635 | 1.373 | 32.781 | 1.839 | 1.363 | 234.793 | 1.450 | 0.001 | 0.145 | 0.697 | 107.956 | 0.001 | 0.032 |
LP4 | 29.919 | 1.451 | 2.468 | 31.060 | 0.239 | 3.221 | 2.253 | 43.918 | 2.873 | 1.754 | 193.086 | 2.154 | 0.013 | 0.027 | 0.249 | 36.637 | 0.001 | 0.015 |
LP5 | 43.240 | 2.466 | 2.331 | 46.991 | 0.209 | 3.218 | 1.577 | 26.153 | 1.994 | 2.211 | 234.265 | 1.895 | 0.010 | 0.017 | 0.224 | 84.449 | 0.004 | 0.006 |
LP6 | 29.925 | 2.796 | 2.933 | 11.764 | 0.248 | 2.974 | 2.174 | 43.896 | 2.394 | 1.784 | 247.178 | 3.487 | 0.012 | 0.017 | 0.336 | 125.785 | 0.003 | 0.016 |
LP7 | 28.783 | 1.090 | 2.067 | 41.129 | 0.244 | 2.388 | 1.514 | 76.640 | 1.923 | 1.886 | 157.760 | 2.486 | 0.003 | 0.031 | 0.241 | 87.240 | 0.004 | 0.053 |
LP8 | 45.076 | 3.030 | 1.857 | 7.724 | 0.247 | 2.328 | 2.152 | 87.415 | 2.455 | 2.044 | 225.691 | 2.976 | 0.011 | 0.009 | 0.607 | 111.718 | 0.003 | 0.023 |
LP9 | 20.651 | 2.339 | 2.731 | 42.403 | 0.257 | 1.681 | 1.012 | 89.960 | 1.066 | 1.855 | 210.521 | 2.237 | 0.007 | 0.052 | 0.424 | 32.520 | — | 0.033 |
LP10 | 23.769 | 1.646 | 1.288 | 52.927 | 0.243 | 2.319 | 2.950 | 93.752 | 2.929 | 1.882 | 217.655 | 2.496 | 0.017 | 0.165 | 0.196 | 135.624 | 0.003 | 0.051 |
LP11 | 32.364 | 2.560 | 3.084 | 33.335 | 0.208 | 3.219 | 2.180 | 82.812 | 1.451 | 1.918 | 269.769 | 3.250 | 0.019 | 0.008 | 0.406 | 66.278 | 0.003 | 0.051 |
LP12 | 24.918 | 1.470 | 2.999 | 37.041 | 0.243 | 1.893 | 1.928 | 95.305 | 2.415 | 1.698 | 224.103 | 1.906 | 0.008 | 0.041 | 0.570 | 64.033 | 0.004 | 0.008 |
LP13 | 33.468 | 1.704 | 2.451 | 45.575 | 0.176 | 2.507 | 1.882 | 38.837 | 2.524 | 1.580 | 187.087 | 1.245 | 0.003 | 0.133 | 0.285 | 44.075 | 0.003 | 0.030 |
LP14 | 28.614 | 2.426 | 1.879 | 39.614 | 0.209 | 3.272 | 1.963 | 74.622 | 2.875 | 1.962 | 261.572 | 2.514 | 0.013 | 0.136 | 0.287 | 100.138 | 0.002 | 0.048 |
LP15 | 35.617 | 2.088 | 2.116 | 42.214 | 0.162 | 2.284 | 1.641 | 39.037 | 1.412 | 1.185 | 244.588 | 0.489 | 0.007 | 0.158 | 0.512 | 110.953 | 0.003 | 0.032 |
LP16 | 32.522 | 2.845 | 1.545 | 41.087 | 0.186 | 2.100 | 1.860 | 94.105 | 3.060 | 1.921 | 165.053 | 1.398 | 0.015 | 0.008 | 0.451 | 69.796 | 0.002 | 0.066 |
LA1 | 36.451 | 2.711 | 1.271 | 38.156 | 0.233 | 2.893 | 0.909 | 65.183 | 3.041 | 1.926 | 194.455 | 3.342 | 0.005 | 0.055 | 0.262 | 51.502 | 0.002 | 0.023 |
LA2 | 43.394 | 2.188 | 2.067 | 17.427 | 0.165 | 2.206 | 1.564 | 58.425 | 3.159 | 1.247 | 254.469 | 1.789 | 0.006 | 0.146 | 0.280 | 33.628 | 0.003 | 0.052 |
LA3 | 24.311 | 1.120 | 2.917 | 35.712 | 0.247 | 2.359 | 2.087 | 59.228 | 3.345 | 2.092 | 247.432 | 1.778 | 0.010 | 0.108 | 0.362 | 105.945 | — | 0.069 |
LA4 | 26.794 | 1.110 | 1.710 | 13.192 | 0.232 | 1.909 | 1.963 | 25.002 | 3.351 | 1.618 | 198.614 | 1.801 | — | 0.011 | 0.452 | 33.107 | 0.003 | 0.024 |
LA5 | 39.248 | 2.722 | 1.585 | 24.608 | 0.203 | 2.755 | 2.186 | 68.836 | 1.896 | 1.634 | 279.990 | 3.413 | 0.016 | 0.037 | 0.213 | 37.247 | 0.004 | 0.077 |
LA6 | 20.981 | 2.024 | 1.392 | 25.368 | 0.248 | 2.878 | 2.542 | 45.526 | 1.863 | 1.095 | 215.126 | 3.019 | 0.009 | 0.148 | 0.651 | 117.017 | 0.003 | 0.010 |
LA7 | 30.279 | 1.148 | 2.330 | 62.335 | 0.231 | 2.772 | 2.791 | 44.135 | 2.816 | 1.556 | 187.777 | 3.290 | 0.012 | 0.151 | 0.436 | 36.940 | 0.003 | 0.041 |
LA8 | 39.053 | 0.971 | 3.084 | 15.630 | 0.243 | 3.097 | 1.738 | 42.571 | 2.567 | 1.411 | 231.825 | 2.281 | 0.010 | 0.013 | 0.499 | 33.670 | 0.003 | 0.053 |
LA9 | 41.198 | 2.925 | 2.254 | 52.257 | 0.230 | 2.490 | 2.132 | 95.196 | 2.872 | 2.220 | 170.239 | 1.302 | 0.002 | 0.132 | 0.395 | 80.576 | 0.003 | 0.015 |
LA10 | 16.833 | 1.384 | 2.832 | 34.567 | 0.188 | 2.044 | 1.951 | 62.570 | 0.922 | 1.571 | 169.715 | 0.952 | 0.001 | 0.110 | 0.496 | 81.418 | 0.002 | 0.044 |
LA11 | 38.564 | 2.900 | 3.171 | 30.344 | 0.257 | 2.860 | 2.083 | 37.280 | 1.155 | 1.073 | 172.207 | 1.959 | 0.008 | 0.117 | 0.433 | 108.524 | 0.002 | 0.014 |
LA12 | 43.364 | 1.975 | 2.442 | 7.660 | 0.253 | 1.986 | 0.775 | 85.359 | 2.247 | 1.143 | 237.191 | 0.673 | 0.009 | 0.155 | 0.201 | 41.025 | — | 0.002 |
LA13 | 30.181 | 3.229 | 1.146 | 53.644 | 0.230 | 2.341 | 2.091 | 77.183 | 0.842 | 2.028 | 177.614 | 2.395 | 0.009 | 0.105 | 0.689 | 97.023 | 0.001 | 0.022 |
LA14 | 31.940 | 3.492 | 1.508 | 64.537 | 0.181 | 2.947 | 2.883 | 24.336 | 3.049 | 2.137 | 243.782 | 1.989 | 0.017 | 0.117 | 0.272 | 125.192 | 0.003 | 0.021 |
LA15 | 22.170 | 2.327 | 3.077 | 51.812 | 0.175 | 3.050 | 0.998 | 60.176 | 2.860 | 1.716 | 206.597 | 0.281 | 0.006 | 0.058 | 0.397 | 108.215 | — | 0.025 |
LA16 | 42.638 | 1.570 | 1.978 | 49.228 | 0.258 | 1.959 | 0.924 | 88.563 | 2.498 | 1.683 | 201.422 | 3.242 | 0.014 | 0.029 | 0.295 | 80.373 | 0.003 | 0.075 |
LA17 | 39.670 | 1.249 | 2.650 | 27.755 | 0.259 | 2.112 | 2.250 | 55.219 | 2.830 | 1.217 | 154.053 | 1.978 | 0.006 | 0.099 | 0.561 | 116.452 | — | 0.047 |
LA18 | 23.423 | 2.528 | 2.932 | 10.179 | 0.199 | 2.743 | 2.953 | 66.269 | 3.199 | 1.527 | 209.326 | 3.075 | 0.016 | 0.116 | 0.306 | 79.441 | 0.001 | 0.040 |
Sample | Na | Mg | Al | K | Ca | Fe | N | P | Total | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
SP1 | 410579.062 | 209771.718 | 18661.431 | 178472.536 | 591631.101 | 334974.476 | 4.303 | 0.914 | 1744628.970 | |||||||||
SP2 | 546214.809 | 232797.639 | 9209.284 | 416708.514 | 386703.829 | 349989.801 | 2.389 | 0.675 | 1942044.979 | |||||||||
SP3 | 384732.341 | 302374.639 | 16864.219 | 351597.651 | 645593.344 | 330485.336 | 18.804 | 5.589 | 2032063.470 | |||||||||
SP4 | 763978.060 | 217006.139 | 24174.116 | 515345.319 | 525036.011 | 165789.077 | 19.687 | 4.182 | 2211765.493 | |||||||||
SP5 | 498927.575 | 304758.187 | 29743.190 | 617594.946 | 663524.773 | 200323.584 | 25.750 | 23.839 | 2315416.327 | |||||||||
SP6 | 568688.625 | 219822.814 | 31062.346 | 305039.982 | 486491.766 | 202085.631 | 25.562 | 22.681 | 1813617.704 | |||||||||
SP7 | 815621.400 | 203217.299 | 32660.784 | 559562.408 | 566642.730 | 171755.702 | 2.565 | 1.507 | 2349855.405 | |||||||||
SP8 | 704283.730 | 188285.004 | 23602.584 | 315772.349 | 624642.806 | 439955.497 | 23.372 | 6.689 | 2297077.677 | |||||||||
SP9 | 876213.262 | 151574.474 | 27629.879 | 516294.381 | 409003.501 | 35506.241 | 18.458 | 4.258 | 2016572.669 | |||||||||
SP10 | 873643.979 | 294027.085 | 21164.307 | 737471.818 | 383002.078 | 37324.576 | 33.550 | 19.033 | 2347111.719 | |||||||||
SP11 | 491391.823 | 256934.016 | 10147.423 | 610308.309 | 636084.667 | 367347.644 | 31.694 | 25.205 | 2372762.062 | |||||||||
SP12 | 796497.255 | 269227.175 | 17999.811 | 414658.818 | 512138.780 | 171064.616 | 16.646 | 2.703 | 2182110.814 | |||||||||
SP13 | 366808.463 | 156147.673 | 11576.232 | 471660.374 | 756208.109 | 347515.518 | 11.647 | 4.202 | 2110408.155 | |||||||||
SP14 | 512150.165 | 243500.636 | 31493.169 | 641312.090 | 499270.353 | 208734.109 | 11.077 | 1.602 | 2136861.051 | |||||||||
SP15 | 533557.790 | 280721.134 | 16614.646 | 267728.355 | 378226.451 | 306162.902 | 13.593 | 4.564 | 1783528.184 | |||||||||
SP16 | 644403.624 | 276057.049 | 21110.856 | 521532.157 | 583524.045 | 59176.189 | 11.942 | 25.264 | 2106319.987 | |||||||||
SP17 | 751229.025 | 173163.933 | 10495.020 | 625004.125 | 472688.229 | 345116.023 | 8.458 | 5.204 | 2378144.753 | |||||||||
SP18 | 522140.520 | 176639.957 | 29371.612 | 557932.109 | 690911.336 | 157124.877 | 29.906 | 10.221 | 2134563.150 | |||||||||
SP19 | 579415.759 | 238453.094 | 30677.565 | 439717.245 | 686006.551 | 382672.972 | 10.823 | 3.662 | 2357515.153 | |||||||||
SP20 | 476281.317 | 182915.790 | 9070.049 | 325811.742 | 373586.252 | 432553.750 | 34.616 | 21.891 | 1800784.763 | |||||||||
SP21 | 760676.992 | 188574.381 | 12126.295 | 629919.272 | 484913.883 | 230880.412 | 26.927 | 9.833 | 2307491.687 | |||||||||
SP22 | 709408.940 | 239417.173 | 10783.138 | 619462.993 | 550226.213 | 88795.927 | 20.170 | 9.877 | 2218626.941 | |||||||||
SP23 | 513303.754 | 213899.504 | 26441.948 | 351694.267 | 732144.084 | 326876.462 | 34.159 | 26.699 | 2164823.520 | |||||||||
SP24 | 824465.276 | 238490.190 | 31283.397 | 717257.458 | 679154.325 | 297001.594 | 14.031 | 10.796 | 2788075.528 | |||||||||
SP25 | 815267.536 | 215764.623 | 30718.721 | 536395.881 | 508251.297 | 371174.761 | 24.650 | 2.494 | 2478087.325 | |||||||||
LP1 | 897950.747 | 158686.979 | 27871.364 | 590366.194 | 696960.175 | 327232.239 | 17.263 | 7.080 | 2699502.116 | |||||||||
LP2 | 531566.916 | 294929.433 | 14640.021 | 570417.870 | 648189.841 | 273059.464 | 15.536 | 1.239 | 2333208.859 | |||||||||
LP3 | 858626.936 | 266231.149 | 32272.481 | 727712.714 | 480257.660 | 348345.090 | 12.319 | 1.894 | 2713926.251 | |||||||||
LP4 | 431098.253 | 221743.162 | 34467.794 | 311381.975 | 785570.359 | 339960.811 | 26.584 | 17.225 | 2124617.501 | |||||||||
LP5 | 818404.736 | 237360.411 | 25820.309 | 683695.992 | 566015.946 | 406722.563 | 20.219 | 7.521 | 2738498.958 | |||||||||
LP6 | 614726.783 | 203772.151 | 23626.598 | 170198.351 | 715325.262 | 132978.487 | 8.402 | 1.447 | 1861115.202 | |||||||||
LP7 | 498622.492 | 200069.361 | 34249.218 | 182114.487 | 450498.128 | 213156.897 | 26.337 | 12.664 | 1579155.065 | |||||||||
LP8 | 407734.217 | 308322.609 | 12414.630 | 462642.984 | 726996.054 | 273473.025 | 23.952 | 9.428 | 2192112.264 | |||||||||
LP9 | 591923.425 | 196758.141 | 20795.603 | 169080.807 | 379447.395 | 244832.850 | 24.638 | 15.719 | 1603288.325 | |||||||||
LP10 | 506188.280 | 278745.249 | 26183.977 | 548614.526 | 619327.368 | 71487.282 | 27.250 | 10.475 | 2051124.319 | |||||||||
LP11 | 484360.416 | 252740.828 | 27267.497 | 394747.762 | 420528.173 | 411590.345 | 15.427 | 3.462 | 1991756.825 | |||||||||
LP12 | 755545.368 | 290158.455 | 18713.693 | 269669.218 | 653034.467 | 303936.794 | 26.120 | 0.889 | 2291545.587 | |||||||||
LP13 | 644431.270 | 171083.444 | 14922.153 | 701260.007 | 799614.153 | 241821.499 | 27.383 | 10.915 | 2573534.387 | |||||||||
LP14 | 855511.785 | 226105.300 | 11812.507 | 382201.190 | 657443.790 | 421778.147 | 4.108 | 0.257 | 2555378.850 | |||||||||
LP15 | 840333.425 | 261183.216 | 19461.126 | 468066.062 | 568653.040 | 96502.984 | 17.469 | 12.422 | 2254714.244 | |||||||||
LP16 | 662138.232 | 247763.574 | 23217.103 | 653430.420 | 481555.835 | 52632.672 | 16.299 | 0.242 | 2121172.397 | |||||||||
LA1 | 542867.038 | 291631.774 | 16101.327 | 208393.119 | 534546.718 | 287585.245 | 20.487 | 11.217 | 1881559.346 | |||||||||
LA2 | 768502.457 | 174767.313 | 34293.763 | 538598.282 | 767009.154 | 130620.652 | 24.093 | 8.098 | 2414246.025 | |||||||||
LA3 | 836100.485 | 232547.859 | 8383.317 | 197659.044 | 475008.913 | 70420.274 | 26.668 | 18.771 | 1820654.452 | |||||||||
LA4 | 554969.006 | 231606.445 | 11451.728 | 365538.304 | 505163.079 | 269818.459 | 11.932 | 3.908 | 1938873.753 | |||||||||
LA5 | 794677.014 | 293809.893 | 30832.050 | 293530.490 | 510493.154 | 262663.280 | 12.498 | 6.307 | 2186491.358 | |||||||||
LA6 | 704360.729 | 287183.610 | 31019.483 | 189142.179 | 384371.287 | 327789.933 | 12.239 | 4.852 | 1924324.212 | |||||||||
LA7 | 894339.623 | 204701.453 | 27985.214 | 281212.437 | 595254.900 | 371594.312 | 24.934 | 18.350 | 2375510.268 | |||||||||
LA8 | 427283.433 | 184599.086 | 23700.009 | 211637.098 | 596714.072 | 325251.967 | 22.097 | 1.616 | 1769588.097 | |||||||||
LA9 | 879491.821 | 180076.383 | 12682.543 | 611024.725 | 416252.729 | 426535.290 | 22.887 | 10.851 | 2526553.669 | |||||||||
LA10 | 712373.467 | 297199.560 | 9966.649 | 218385.532 | 678770.388 | 129164.596 | 32.940 | 4.216 | 2046274.948 | |||||||||
LA11 | 625486.302 | 275066.946 | 25810.603 | 496586.040 | 470812.328 | 438093.869 | 14.927 | 1.233 | 2332275.197 | |||||||||
LA12 | 796469.226 | 199565.531 | 26532.869 | 616737.846 | 538184.180 | 286481.065 | 25.413 | 16.087 | 2464438.679 | |||||||||
LA13 | 587462.535 | 234893.095 | 16533.152 | 573600.610 | 441381.833 | 215218.753 | 5.375 | 3.154 | 2069549.281 | |||||||||
LA14 | 598797.362 | 200101.308 | 32285.037 | 179408.885 | 737688.278 | 321911.868 | 10.937 | 7.808 | 2070719.888 | |||||||||
LA15 | 775176.535 | 182419.544 | 9875.652 | 375172.731 | 686178.073 | 146549.529 | 20.767 | 18.862 | 2175875.636 | |||||||||
LA16 | 486462.514 | 269482.486 | 19514.461 | 382028.835 | 385846.710 | 419515.606 | 25.566 | 4.690 | 1963357.619 | |||||||||
LA17 | 397975.248 | 220647.104 | 20225.722 | 404790.872 | 406870.509 | 69049.196 | 25.782 | 16.448 | 1520009.288 | |||||||||
LA18 | 852308.317 | 145491.528 | 11612.476 | 499143.838 | 570244.949 | 84828.689 | 23.929 | 16.569 | 2164078.568 |
—, analytes were not detected.
The elements with higher content in the samples were sodium, calcium, potassium, iron, magnesium, and aluminum. Their concentration ranges were 366808.463–897950.747, 373586.252–799614.153, 169080.807–737471.818, 35506.241–439955.497, 145491.528–308322.609, and 8383.317–34467.794
As one of the most important multivariate analysis techniques, PCA was employed to evaluate the variation among three species of duckweed. PCA is an unsupervised pattern recognition method without prior information of the data set and retained maximum variance of multidimensional data while reducing its dimensionality into two- or three-dimension [
Three-dimensional score plot of principle component analysis (PCA) based on the content of free amino acids.
Three-dimensional score plot of orthogonal partial least square-discriminant analysis (OPLS-DA) based on the content of free amino acids.
Three-dimensional score plot of principle component analysis (PCA) based on the ratio of free to total amino acids.
Three-dimensional score plot of orthogonal partial least squares-discriminant analysis (OPLS-DA) based on the ratio of free to total amino acids.
In this study, a simple, fast, and convenient method with UPLC-QTRAP-MS/MS was established for the identification and quantification control of duckweeds by HILIC separation without derivatization. The results of method validation suggested that the developed method was sensitive, accurate, and precise for determination of 24 free amino acids. 59 batches of sample in three species were quantified, and then the data were statistically analysed by using PCA and OPLS-DA. The multivariate analysis illustrated that the content of free amino acids was not significantly different among
The data used to support the findings of this study are included within the article.
The authors declare that there are no conflicts of interest regarding the publication of this paper.
This study was supported by the Chinese National Natural Science Foundation (81073002), the Program of Collaborative Innovation Center of Chinese Medicinal Material Resources Industrialization of Jiangsu Province (2016), and the National “Twentieth Five-Year” Technology Support Program (2011BAI04B06).