Downmodulation of Vaccine-Induced Immunity and Protection against the Intracellular Bacterium Francisella tularensis by the Inhibitory Receptor FcγRIIB

Fc gamma receptor IIB (FcγRIIB) is the only Fc gamma receptor (FcγR) which negatively regulates the immune response, when engaged by antigen- (Ag-) antibody (Ab) complexes. Thus, the generation of Ag-specific IgG in response to infection or immunization has the potential to downmodulate immune protection against infection. Therefore, we sought to determine the impact of FcγRIIB on immune protection against Francisella tularensis (Ft), a Category A biothreat agent. We utilized inactivated Ft (iFt) as an immunogen. Naïve and iFt-immunized FcγRIIB knockout (KO) or wildtype (WT) mice were challenged with Ft-live vaccine strain (LVS). While no significant difference in survival between naïve FcγRIIB KO versus WT mice was observed, iFt-immunized FcγRIIB KO mice were significantly better protected than iFt-immunized WT mice. Ft-specific IgA in serum and bronchial alveolar lavage, as well as IFN-γ, IL-10, and TNF-α production by splenocytes harvested from iFt-immunized FcγRIIB KO, were also significantly elevated. In addition, iFt-immunized FcγRIIB KO mice exhibited a reduction in proinflammatory cytokine levels in vivo at 5 days after challenge, which correlates with increased survival following Ft-LVS challenge in published studies. Thus, these studies demonstrate for the first time the ability of FcγRIIB to regulate vaccine-induced IgA production and downmodulate immunity and protection. The immune mechanisms behind the above observations and their potential impact on vaccine development are discussed.


Introduction
Fc gamma receptor IIB (Fc RIIB), which is expressed on B cells, as well as other antigen (Ag) presenting cells (APC), is the only Fc gamma receptor (Fc R) known to negatively regulate the immune response and play an important role in B-cell regulation and antibody (Ab) production [1][2][3][4][5][6][7]. Thus, Ag-specific Ab generated in response to infection, immunization, or the administration of Fc R-targeted vaccines, when complexed with Ag, has the potential to interact with Fc RIIB and significantly downmodulate immunity and protection against infectious agents. Importantly, Fc RIIB is also expressed on dendritic cells (DCs) and macrophages and can negatively regulate cellular as well as humoral immunity [1-3, 6, 8]. Consistent with the regulation of cellular immunity, it has been demonstrated that utilizing a Mycobacterium tuberculosis (MTB) model in which naïve wildtype (WT) and Fc RIIB knockout (KO) mice received an aerosol challenge of MTB, Fc RIIB KO mice exhibited an enhanced cellular immune response compared to their WT counterparts, which included increased IFN-production [9]. In another study, following Staphylococcus aureus (SA) infection, naïve Fc RIIB KO mice were better protected against a primary 2 Journal of Immunology Research intravenous infection with SA [10]. However, in contrast to the above results using naïve mice, if Fc RIIB KO mice were first immunized with a pneumococcal vaccine and then challenged with high doses of Streptococcus pneumoniae, mortality rates were increased above that of WT mice, correlating with increased proinflammatory cytokine production [11]. Yet, in another study in which Plasmodium berghei parasitemia was induced postimmunization, parasitemia was not impacted following intradermal immunization of Fc RIIB KO versus WT mice with a Plasmodium berghei vaccine [12]. Thus, while enhanced immunity and/or protection against infection was observed in naïve Fc RIIB KO versus WT mice, the absence of Fc RIIB had either negative or no impact on immunity and/or protection following immunization.
To further clarify the role of Fc RIIB in the generation of protective immunity against infection, we investigated the impact of Fc RIIB on F. tularensis (Ft) challenge before and after immunization with inactivated Ft (iFt) in WT versus Fc RIIB KO mice. Ft is a gram-negative intracellular pathogen that in designated a Category A biothreat agent due to its extreme virulence [13][14][15]. Based on the published studies cited above using naïve Fc RIIB KO mice [9][10][11], we hypothesized that naïve mice challenged with Ft would be better protected in the absence of Fc RIIB. In contrast, based on the published studies above using immunized Fc RIIB KO mice [11,12], we predicted a negative or very limited impact of the absence of Fc RIIB on survival following Ftchallenge of iFt-immunized Fc RIIB KO versus WT mice. In fact, the opposite was observed in the case of both naïve and iFt-immunized mice. Specifically, there was no difference in survival of naïve Fc RIIB KO versus WT mice challenged with Ft. In contrast, in the case of iFt-immunized Fc RIIB KO versus WT mice, protection was significantly improved in Fc RIIB KO mice. The latter correlated with enhanced Ft-specific IgA production in vivo, enhanced recall responses in the form of increased IFN-production by ex vivo splenocytes incubated with iFt, and reduced in vivo proinflammatory cytokine production 5 days after challenge. Thus, these studies demonstrate for the first time that the presence of Fc RIIB can significantly dampen immunity and protection against infection following immunization. These are also the first studies to suggest a role of Fc RIIB in the regulation of Ag-specific IgA production. Potential explanations for the above observations are discussed. Louis, Missouri), and 10 mL isovitalex (Becton Dickinson, Sparks, Maryland). MHB media was adjusted to a pH of 6.8, filtered (0.22 m), and stored at 4 ∘ C for up to two weeks. Red blood cell (RBC) lysis buffer contained 4.13 g of ammonium chloride (Sigma-Aldrich, St. Louis, Missouri), 0.5 g of potassium bicarbonate (Sigma-Aldrich, St. Louis, Missouri), and 18.5 mg of EDTA (Sigma-Aldrich, St. Louis, Missouri) diluted in 500 mL of distilled water. The pH of the solution was adjusted to 7.2, after which the solution was filter-sterilized using a 0.22 m filter and stored at 4 ∘ C.

Splenocyte
Isolation. Spleens were isolated from mice and passed through a 70 m cell strainer (Fisherbrand, Houston, TX). The single cell suspension was collected in a sterile petri dish containing approximately 3 to 5 mL of media. The cell suspension was washed and RBCs were lysed using a RBC lysis buffer. The cell suspension was then passed through a second 70 m cell strainer (Fisherbrand, Houston, TX) and collected in a 50 mL conical centrifuge tube containing approximately 10 mL of media. Cells were spun for 5 minutes at 1500 revolutions per minute (RPM) and resuspended in RBC lysis buffer. After 3 to 5 minutes (min), the reaction was quenched through the addition of 20 to 40 mL of media. Splenocytes were again spun for 5 min at 1500 RPM. Following resuspension, any residual tissue was removed via pipet or by again passing the splenocyte suspension through a 70 m filter (Fisherbrand, Houston, TX). Splenocytes were subsequently washed twice with fresh media twice and enumerated using trypan blue.

2.3.
Mice. C57BL/6J mice were obtained from Jackson Laboratories (Bar Harbor, ME). Breeding pairs of Fc RIIB KO mice (B6; 129S4-Fcgr2b tm1Ttk /J) on a C57BL background were obtained from Jackson Laboratories and bred in the Animal Resources Facility at Albany Medical College. Mice of 6-15 weeks old were used in order to identify significant differences under conditions that include a relatively broad range of ages. All animals were housed and cared for according to guidelines approved by the Institutional Animal Care and Use Committee.

Immunization and Challenge Studies.
Mice were divided into groups consisting of 6-8 mice per group. Each mouse was anesthetized by intraperitoneal (i.p.) injection of 20% ketamine (Vedco, St. Joseph, Missouri) plus 5% xylazine (Lloyd, Shenandoah, Iowa) diluted in sterile cell culture grade water (Cellgro, Manassas, Virginia). Mice were subsequently administered i.n. either 20 L of PBS (vehicle) or 2 × 10 7 iFtorganisms in 20 L of PBS. Unless otherwise indicated, mice were immunized on day 0 and boosted on day 21. Immunized mice were then challenged on day 35 i.n. with either 4 × LD 50 or 16 × LD 50 of live Ft-LVS and subsequently monitored for survival for a minimum of 21 days.

Bacterial Burden Quantitation.
Mice were immunized and subsequently challenged with a sublethal dose (approximately 0.4 × LD 50 ) of live Ft-LVS. Five days after challenge, mice were sacrificed and tissues were collected. Tissues were homogenized with a mechanical bead beater and serially diluted with sterile PBS. Diluted tissue samples were plated on chocolate agar and CFU colonies were enumerated approximately 72 hours later.

Antibody Quantitation.
Total mouse IgM, IgG, and IgA were measured by ELISA. Kits were purchased (Immunology Consultants Laboratory, Portland, OR) and the manufacturers protocol was followed. All reagents were brought to room temperature. Samples and standards were serially diluted and 100 L/well were added to plates precoated with anti-IgM, anti-IgG, or anti-IgA Abs. Plates were incubated at room temperature for 60 minutes. Following the incubation, plates were aspirated and washed, and 100 L of the appropriate enzyme-Ab conjugate was added to each well. After 30 minutes, the plates were again washed and substrate solution was added. The reaction was stopped after 10 minutes with stop solution and the plates were read at 450 nm using a microplate reader (VersaMax, Molecular Devices, Sunnyvale, California). Ft-specific Ab production was also measured by ELISA. ELISA plates (Corning, Corning, New York) were coated with 50 L/well of live Ft-LVS (5 × 10 7 CFU/mL in carbonate buffer containing 2.15 g sodium bicarbonate and 2.62 g sodium carbonate (Sigma-Aldrich, St. Louis, Missouri) diluted in 500 mL of sterile cell culture grade water (Cellgro, Manassas, Virginia) at pH 9.6-9.8), overnight at 4 ∘ C. The plates were then washed three times with 200 L/well of PBS containing 0.5% bovine serum albumin (BSA) (Baxter Healthcare, Deerfield, Illinois) and 0.002% sodium azide (Sigma-Aldrich, St. Louis, Missouri). Plates were then blocked at 4 ∘ C for 2 hours with 200 L/well of PBS containing 5% BSA and 0.02% sodium azide. Plates were again washed and serial dilutions of sera or bronchoalveolar lavage fluids (BALF) were added to the plates (50 L/well) and incubated for 2 hours at 4 ∘ C. After washing, alkaline phosphatase conjugated anti-mouse Ab specific for total mouse Ab (Sigma-Aldrich, St. Louis, Missouri), IgG (Sigma-Aldrich, St. Louis, Missouri), IgA (Sigma-Aldrich, St. Louis, Missouri), IgG1 (Southern Biotech, Birmingham, AL), IgG2c (Abcam, Cambridge, MA), or IgM (Sigma-Aldrich, St. Louis, Missouri) were added and incubated for 1 hour at 4 ∘ C. Plates were washed and then 100 L/well of alkaline phosphatase substrate (Sigma, St. Louis, MO) was added. All samples were read at 405 nm using a microplate reader (VersaMax, Molecular Devices, Sunnyvale, California) following a 5second (sec) shake.

Adoptive Transfer Studies.
Mice were immunized with iFt i.n. as described above and on day 35 mice were anesthetized and blood was collected via percutaneous cardiac puncture. Clotted blood samples were then spun at 8000 RPM for 10 minutes and serum was harvested and pooled. Following heat (complement) inactivation at 55 ∘ C for 30 minutes, serum was spun at 4000 RPM for 10 minutes, aliquoted, and frozen at −20 ∘ C until use. For adoptive transfer experiments, mice were administered i.p. 250 L of PBS (vehicle), serum from PBS immunized mice, or serum iFt-immunized mice. Mice were then anesthetized and challenged with the indicated dose of Ft-LVS 24 hours after adoptive transfer. Survival was monitored for 21 days.

Ex Vivo Splenocyte Activation (Recall Response) Assay.
On day 35 after primary immunization, spleen cells were harvested and diluted in medium to a concentration of 5 × 10 6 spleen cells/mL. 1000 L of cells was added to wells of a 24-well plate (Costar Corning, Corning, NY) plate, each well containing an equivalent number of iFt-organisms. Alternatively 100 L of cells was added to wells of a Ubottom 96-well plate (Falcon BD, Franklin Lakes, NJ) that contained a concentration range between 2.5 and 20 iFt per splenocyte. Plates were incubated at 37 ∘ C for up to 7 days in a humidity chamber to prevent medium evaporation. Supernatants were collected at 1, 3, 5, and 7 days and frozen at −20 ∘ C until they were analyzed. Samples were analyzed for cytokines via cytometric bead array (CBA) multiplex assay (BD Biosciences-BD Pharmingen, Sparks, Maryland). Data was acquired on a FACSArray Instrument and analyzed using CBA software version 1.0.1 (BD Immunocytometry Systems, Sparks, Maryland).

Cytokine Quantitation in Lungs and BALF. WT and
Fc RIIB KO mice were immunized i.n. and on day 35 mice were given a sublethal challenge i.n. with approximately 0.4 × LD 50 of Ft-LVS. Tissues were collected five days after challenge. Tissues were homogenized and centrifuged, and supernatants were collected. Luminex assay was performed on tissue supernatants to determine in vivo cytokine levels and to assess inflammation. The Luminex assay is a multiplex system for quantitation and detection of multiple cytokines in a single sample.

Statistical
Analysis. The method of statistical analysis for each figure is described in the respective figure legends.  [16]. We also examined serum levels of total IgM and total IgA, as well as Ft-specific IgM and IgG, since mice have propensity for low basal levels of natural Ft-specific Ab [17]. As shown in Table 1 [18][19][20]. Thus, we examined the production of Ft-specific Ab, including IgG, IgA, and IgM, in the serum and BALF of Fc RIIB KO versus WT mice immunized with iFt. In some cases, the titers obtained were highly variable between individual mice, which likely reflects not only normal mouse-to-mouse variation but also differences in immune responsiveness due to differences in the age of the individual mice and its effects on B cell repertoires [21]. Despite the latter, we did observe a significant increase in the production of total Ft-specific serum Ab in Fc RIIB KO versus WT mice following iFt-immunizations (Figure 2(a)). While there were no significant differences in the levels of Ft-specific serum IgG, IgG2c, IgG1, or IgM in the serum of these mice (Figures 2(b), 2(c), 2(d), or 2(e), resp.), Ft-specific IgA levels in serum were significantly increased (Figure 2(f)). While there is not a dramatic increase in the median response in total anti-Ft Ab titer between PBS and iFt-immunized Fc RIIB KO mice (Figure 2(a)), there is a significant increase in the median levels of IgG2c in iFt-immunized Fc RIIB KO mice versus PBS-immunized Fc RIIB KO mice ( < 0.01) (Figure 2(c)). This suggests that despite the former observation regarding total anti-Ft Ab, iFt-immunized Fc RIIB KO mice produce increased levels of potentially high affinity IgG2c anti-Ft Ab. Lastly, there were no significant differences in the levels of Ft-specific IgG in the BALF of these mice, while the levels of Ft-specific IgA in the BALF of iFt-immunized Fc RIIB KO mice were significantly higher than that of WT mice (Figure 2(h)). The presence of Ft-specific Abs in naïve (Table 1) or unimmunized mice has been previously observed in mouse [17] and humans [22,23] and is likely due to the presence of B1 cells producing natural antibodies stimulated in response to normal flora or self-Ag [24,25].

iFt-Immunized Fc RIIB KO Mice Exhibit Increased Protection against a Lethal Ft-LVS Challenge as Compared to
Their WT Counterparts. Given the integral role Ag-specific IgG plays in Fc RIIB-mediated immune modulation and the absence of increased levels of Ft-specific IgG in iFtimmunized Fc RIIB KO versus WT mice (Figure 2), we sought to determine whether there would be any impact of the presence versus absence of Fc RIIB on protection of iFt-immunized mice. Despite the lack of a significant increased Ft-specific IgG in Fc RIIB KO versus WT mice and in contrast to studies using naïve mice (Figure 1), at a challenge dose of 4 × LD 50 , the survival of iFt-immunized Fc RIIB KO mice was significantly better than that of WT mice (100% versus 50% resp.) (Figure 3(a)). Similar to that observed in naïve mice (Figure 1), there was no significant difference in survival between Fc RIIB KO and WT mice immunized with PBS (Figure 3(a)). When the challenge dose was increased to 16 × LD 50 , the overall level of survival decreased. However, a slight increase in the survival of iFtimmunized Fc RIIB KO versus WT mice was still apparent (Figure 3(b)). The increased survival also correlated with a reduction in the median bacterial burden in iFt-immunized Fc RIIB KO (Figure 3(c)).

Adoptive Transfer of Serum from iFt-Immunized Fc RIIB KO and WT Mice to Naïve Fc RIIB KO and WT Mice, Respectively, Does Not Replicate the Differences in Survival Observed in iFt-Immunized Fc RIIB KO versus WT Mice.
We surmised that while there were no differences in Ftspecific IgG levels in iFt-immunized Fc RIIB KO versus WT mice (Figure 2), the observed increases in Ft-specific IgA in the serum and BALF of Fc RIIB KO versus WT mice might be responsible for the increased protection observed (Figure 3). To test this, we adoptively transferred serum from iFt-immunized Fc RIIB KO and WT mice into their naive counterparts, in an effort to recapitulate the difference in survival obtained in Figure 3. As demonstrated previously by others [26], adoptive transfer of serum to naïve mice did increase protection against Ft-LVS challenge. However, a significant increase in protection in naïve Fc RIIB KO mice receiving serum from iFt-immunized Fc RIIB KO mice versus that of WT mice was not observed (Table 2).

Splenocytes from iFt-Immunized Fc RIIB KO versus WT Mice Exhibit Enhanced IFN-, IL-10, and TNF-Production in Response to iFt Added Ex Vivo.
Given that IFN-, a Th1 cytokine, has been shown to play an important role in protection against Ft-infection [13,[27][28][29][30] and that Fc RIIB has been shown to downmodulate cellular immune responses, including Th1 responses [9,11], we examined the impact of Fc RIIB's absence on the production of IFNby splenocytes from iFt-immunized Fc RIIB KO versus Ft-specific IgM (e), and Ft-specific IgA (f) were determined via ELISA. Titers of Ft-specific IgG (g) and Ft-specific IgA (h) in BALF were similarly determined. Each symbol represents a single mouse. The 50% maximum of the highest experimental OD was determined and all experimental OD values were transformed to their respective log values. A log (agonist) versus response-variable slope (four parameters) nonlinear regression (curve fit) was performed on the log values, and the unknowns were interpolated. The inverse log was taken on the interpolated unknowns to determine the final titers for each individual mouse sample (error bars represent median with interquartile range of data distribution). Statistics were performed by adding 0.1 to the determined titer value and taking the log of this new value to correct for heteroscedasticity. A one-way ANOVA along with a Tukey's multiple comparison test was performed, and median and interquartile range is graphed. * * * < 0.001; * < 0.05.
WT mice restimulated ex vivo with iFt. Consistent with the increased protection observed in iFt-immunized Fc RIIB KO versus WT mice ( Figure 3) and a critical role for IFNin mediating protection against Ft-infection, within three days following restimulation with iFt, splenocytes from iFtimmunized Fc RIIB KO mice exhibited increased levels of IFN-compared to that of WT splenocytes (Figure 4(a)). This remained the case at a series of iFt/splenocyte ratios ranging between 2.5 and 20 : 1 (Figure 4(b)). Furthermore, in this same experiment, we also examined the production of IL-10 and IL-17, since IL-10 restrains IL-17-induced lung pathology following pulmonary Ft-LVS infection [31]. We demonstrate that iFt-immunized Fc RIIB KO mice produce increased levels of IL-10 and lower levels of IL-17A than iFtimmunized WT mice, consistent with the ability of IL-10 to limit IL-17 induced pathology in Fc RIIB KO mice (Figures  4(c) and 4(d)), leading to increased survival ( Figure 3). Finally, we demonstrate that iFt-immunized Fc RIIB KO mice produce higher levels of TNF-compared to iFtimmunized WT mice (Figure 4(e)), another cytokine that also plays an essential role in survival against a primary Ftinfection [32].

Levels of Proinflammatory Cytokines in the Lungs of iFt-Immunized Fc RIIB KO versus WT Mice Were Reduced 5 Days after Challenge.
We have previously observed that although immunization with iFt initially stimulates increased production of inflammatory cytokines, including IFN-, on days 1-3, an overall reduction in such cytokines occurs in vivo between days 5 and 7 after challenge. Furthermore, the latter decrease correlates with increased protection [13,33]. Therefore, we also analyzed inflammatory cytokine levels in the lungs of iFt-immunized Fc RIIB KO versus WT 5-day mice after challenge. Consistent with our prior observations, significantly lower levels of IFN-and MCP-1 in the lungs and TNF-in the BALF of iFt-immunized Fc RIIB KO versus WT mice were observed (Figures 5(c), 5(e), and 5(h), resp.). There were also reductions in the median cytokine levels for IL-6 and TNF-in the lungs of these same mice ( Figures  5(a) and 5(g), resp.), as well as IL-6 ( Figure 5(b)), IFN-( Figure 5(d)), and MCP-1 ( Figure 5(f)) in BALF.

The Impact of Fc RIIB on Primary Infection.
A very limited number of studies thus far have focused on the role of Fc RIIB in resolving primary infections [9][10][11][12]. Specifically, they suggest that, during primary infection, the absence of Fc RIIB results in an improved immune response to infection, which can be beneficial to the host [9][10][11]. In contrast, other studies suggest that, following immunization, the absence of Fc RIIB leads to an overproduction of cytokines and potential septic shock [11] or no differences in immunity and/or protection [11,12]. However, our studies Five days after challenge lungs were collected, homogenized, and plated on chocolate agar to determine bacterial burden as described in Section 2. Each symbol represents a single mouse. Statistical analysis of the tissue bacterial burden was done via a nonparametric one-way ANOVA (Kruskal-Wallis test) coupled with a Dunn's multiple comparison after test. While there appeared to be a substantial reduction in bacterial burden in the majority of iFt-immunized Fc RIIB KO versus WT mice, the difference was not significant based on this analysis. using the Ft-infectious disease model demonstrate that the absence of Fc RIIB has little or no impact on the outcome of survival following primary infection, while its absence following vaccination and challenge increases the protective efficacy of the vaccine. A number of likely explanations exist for our observations. In regard to primary infection of naïve mice, our studies and those of others have demonstrated Fc RIIB deficient mice exhibit higher levels of total IgG [6,16]. Our studies also demonstrate that there is no significant difference in Ft-specific IgG or total IgA Abs in naïve Fc RIIB KO versus WT mice ( Table 1). The latter, in addition to the fact that the formation of Ft-anti-Ft Ab complexes would be necessary to actively engage Fc RIIB and impact immunity and protection, may explain a failure to see differences in protection between naïve Fc RIIB KO and WT mice. Furthermore, the generation of Ft-specific IgG in response to infection would not be expected to occur until approximately day 7 after infection [26], the same point at which mice generally succumb to infection (Figure 1). Five days after challenge lung tissue was collected, homogenized, and centrifuged and supernatants were collected. Luminex assay was performed on tissue supernatants to determine in vivo cytokine levels. Each symbol represents a single mouse. A value of 0.1 was added to the average cytokine concentration to correct for heteroscedasticity. A one-way ANOVA along with a Tukey's multiple comparison test was performed on the log of the average cytokine concentration of lung and BALF samples from individual mice to determine statistical significance, and median and interquartile range is graphed. * < 0.05. S. pneumoniae and subsequent challenge [11], iFt-immunized mice are better protected when Fc RIIB is absent (Figure 3). This is also despite the lack of significant differences in the levels of Ft-specific IgG production in Fc RIIB KO versus WT mice following iFt-immunization ( Figure 2). Importantly, however, both IgG and IgA can mediate protection against Ft-infection [13,29,30,[34][35][36][37][38]. Thus, the significant increase in Ft-specific IgA production in iFt-immunized Fc RIIB KO versus WT mice ( Figure 2) could explain the increased protection observed with iFt-immunized Fc RIIB KO versus WT mice. Never the less, a role for Ft-specific IgA in mediating the increased protection observed (Figure 3) is not supported by adoptive transfer studies. Specifically, no significant difference in protection was observed in naïve recipient Fc RIIB KO versus WT mice following adoptive transfer of sera from iFt-immunized Fc RIIB KO or WT donors, respectively (Table 2). However, cellular immune responses can also play a crucial role in survival against a lethal Ft-challenge [13,[27][28][29][30][34][35][36][37][38]. In this regard, we do show splenocytes from iFt-immunized Fc RIIB KO mice incubated with iFt ex vivo produce substantially more IFN-, IL-10, and TNF-than their WT counterparts, while producing less IL-17, which has been associated with increased pathology (Figure 4) [31]. Thus, this observation more likely explains the enhanced protection observed in iFt-immunized Fc RIIB KO mice. However, a role for Ft-specific IgA cannot be totally excluded, in that our prior studies have demonstrated the requirement for both IgA and IFN-in protection of iFt-immunized mice following Ft-LVS challenge [13,33]. Lastly, our studies, and those of others, have demonstrated that a reduction in the levels of proinflammatory cytokines 5-7 days after challenge, correlates with increased protection in the Ft-infectious disease model [13,29,33,37,39]. Accordingly, we also observed a significant reduction in proinflammatory cytokines in the lungs of iFt-immunized Fc RIIB KO versus WT mice 5 days after challenge ( Figure 5). Furthermore, this decrease in proinflammatory cytokines in lungs of iFt-immunized Fc RIIB KO versus WT mice at 5 days after challenge also correlated with a decrease in bacterial burden in the lungs of these animals, reflecting the ability of iFt-immunized Fc RIIB KO mice to better control Ft-infection than their WT counterparts (Figure 3).

Consideration of Fc RIIB's Regulatory Role in the Development of Vaccines.
We have recently demonstrated that targeting iFt to Fc receptor (FcR) in the form of monoclonal Ab-(mAb-) iFt complex administered i.n., provides superior protection to that of iFt [13]. However, a major concern regarding Fc R-targeted vaccines, when utilizing Fc to target vaccine Ags, is their ability to bind to Fc RIIB, as well as activating Fc Rs, which could potentially dampen the response to vaccination and thereby limit efficacy. Thus, FcR-targeted vaccines that bypass Fc RIIB in favor of activating Fc R, such as one recently developed in our laboratory, which targets the activating Fc R (human Fc RI) [40], could significantly increase the efficacy of such vaccines. Alternatively, as we have demonstrated in this paper, Fc RIIB can also limit the stimulatory capacity of non-FcR-targeted vaccines. Thus, the use of Fc RIIB antagonists as vaccine adjuvants could significantly enhance the efficacy of vaccines in general.

Conclusions
This study is the first to demonstrate that (1) the absence of Fc RIIB does not affect the susceptibility of mice to a primary infection with the intracellular Category A mucosal pathogen F. tularensis; (2) that in the absence of Fc RIIB, both humoral and cellular immunity are enhanced following immunization with the inactivated vaccine iFt; (3) that the level of Ag-specific IgA produced in response to vaccination can be impacted by the presence/absence of Fc RIIB; (4) that the enhanced immune responses observed (Ft-specific IgA and IFN-production) following iFt-immunization correlate with increased protection of Fc RIIB KO versus WT mice after lethal mucosal challenge with Ft LVS. Thus, these studies further expand our knowledge regarding the role of Fc RIIB plays in the immune response to infection, while also providing further impetus for developing vaccines geared toward modulating the inhibitory activities of this receptor.