Effect of HSV-IL12 Loaded Tumor Cell-Based Vaccination in a Mouse Model of High-Grade Neuroblastoma

We designed multimodal tumor vaccine that consists of irradiated tumor cells infected with the oncolytic IL-12-expressing HSV-1 virus, M002. This vaccine was tested against the syngeneic neuroblastoma mouse model Neuro 2a injected into the right caudate nucleus of the immunocompetent A/J mice. Mice were vaccinated via intramuscular injection of multimodal vaccine or uninfected irradiated tumor cells at seven and 14 days after tumor establishment. While there was no survival difference between groups vaccinated with cell-based vaccine applied following tumor injection, a premunition prime/boost vaccination strategy produced a significant survival advantage in both groups and sustained immune response to an intracranial rechallenge of the same tumor. The syngeneic but unrelated H6 hepatocellular tumor cell line grew unrestricted in vaccinated mice, indicative of vaccine-mediated specific immunity to Neuro 2a tumors. Longitudinal analyses of tumor-infiltrating lymphocytes revealed a primary adaptive T cell response involving both CD4+ and CD8+ T cell subsets. Spleen cell mononuclear preparations from vaccinated mice were significantly more cytotoxic to Neuro 2a tumor cells than spleen cells from control mice as demonstrated in a four-hour in vitro cytotoxicity assay. These results strongly suggest that an irradiated whole cell tumor vaccine incorporating IL-12-expressing M002 HSV can produce a durable, specific immunization in a murine model of intracranial tumor.


Introduction
Neuroblastoma (NB) is the most common extracranial tumor diagnosed in children. Widely accepted standard therapy for high-risk NB includes 5 to 7 cycles of intensive cytotoxic chemotherapy, surgery, consolidative autologous stem cell transplantation (SCT), radiation therapy, and maintenance immunotherapy with anti-GD2 antibodies [1]. Such therapy carries considerable toxicity while survival remains generally poor as NB accounts for 15% of deaths attributable to cancer in childhood. Immunotherapy, in particular the use of tumor cell-based vaccines, is an attractive way of generating antineuroblastoma immunity and does not increase the toxicity of concurrent radio-or chemotherapy [2,3].
We have generated a series of cell-based vaccines by combining tumor cells with replication-competent HSV oncolytic virus, which have demonstrated a host immune response following intratumor injection. Based on these findings, we sought to determine the feasibility of an oncolytic HSVbased whole cell peripheral vaccine against an intracranial tumor. We designed a whole cell tumor vaccine to incorporate the oncolytic IL-12-expressing replication-competent HSV-1 M002 into the Neuro 2a (N2a) neuroblastoma cell line derived from A/J mice. We have previously described the M002 HSV-1 in detail [2,4]. In brief, the virus is an attenuated human herpes virus mutant deleted for both copies of the 1 -34.5 gene, restricting virus replication to tumor cells. A genetically concatenated copy of murine IL-12 (p35 and p40 subunits, connected by an IRES) cDNA was inserted into each of the 1 34.5 loci under an Egr1 promoter. Cells infected with this virus make physiologic concentrations of IL-12, a potent antitumor cytokine that has been shown 2 Journal of Immunology Research to enhance the cytolytic activity of natural killer cells and cytotoxic T lymphocytes as well as the development of a TH-1-type immune response [5][6][7][8][9][10][11]. IL-12 has also been shown to produce antiglioma immune activity in two different rodent models and also possesses antiangiogenic properties, an additional potential mechanism for antitumor activity [12,13]. The combination of irradiated tumor cells, oncolytic virus, and local IL-12 production forms a multimodal vaccine approach to malignant CNS tumors that we now seek to apply to neuroblastoma.
In this study, we tested this vaccine against the syngeneic mouse tumor model, Neuro 2a neuroblastoma cell line, in A/J mice in both a postimplantation and a premunition prime/boost vaccination strategy. We also examined whether this vaccination strategy could produce a disease-specific sustained immune response to a rechallenge of the same tumor and whether in vitro cytotoxicity against tumor could be demonstrated from spleen cells of vaccinated mice.

Study Design and Vaccination
Schedule. The study examined potential cancer vaccine settings that address combination therapy with oncolytic virus. Two groups of A/J mice ( = 10 per group) were vaccinated with either irradiated Neuro 2a whole cell (control vaccine (CV)) alone or the complete multimodal vaccine (CV-M002) manufactured as described below. Three control groups received no treatment (tumor control), M002 virus intracranial injection (virus control 1), or M002 virus intracranial injection followed by control vaccine injections (virus control 2) (Figure 2(a)). Mice received 1 × 10 6 vaccine cells (in 50 L aliquots) at three, seven, and fourteen days following tumor implantation, inoculated into the gastrocnemius muscle. Vaccination was repeated as described for each experiment, using the contralateral gastrocnemius muscle from that previously used. Mice were followed up to determine an overall survival. A separate series of studies were undertaken involving vaccination with CV-M002 in mice with intracranial N2a tumors to evaluate tumor infiltration by immune-related cells.
In premunition experiment, a prime-boost strategy was employed (Figure 3(a)) whereby the first dose of complete multimodal vaccine or control vaccine was administered intramuscularly seven days prior to tumor placement and the second dose seven days after. All conditions were compared to an equivalent number of untreated tumor-bearing mice.
The rechallenge with syngeneic related (N2a) or unrelated (H6) tumor cells was performed in mice surviving initial N2a tumor implantation after receiving prime-boost vaccination with multimodal CV-M002. This group was challenged again with N2a cells at day 40 after the first N2a challenge and at day 60 with syngeneic H6 tumor cells to investigate specificity of immune response.

Mice, Tumor Cell Lines, and Virus.
A/J mice, 6-8 weeks old, were purchased from Jackson Laboratories (Bar Harbor, Maine, USA). Mice were housed in a pathogen-free environment in the AALAC-accredited Animal Resource Center at the University of Alabama at Birmingham (UAB). The UAB Institutional Animal Care and Use Committee approved all protocols specific to this study (APN#080607062). The Neuro 2a mouse murine neuroblastoma cell line was originally derived from a spontaneous tumor of the spinal cord in an A/J mouse. We purchased the cell line from the American Type Culture Collection (CCL 131, passage 171). The H6 murine hepatoma of A/J origin was purchased from Jackson Laboratory, DCTD Tumor Repository (MRI Bank #J-750). The M002 virus has been previously described. Cells were maintained in Dulbecco's Modified Eagle's Medium mixed 50 : 50 with Ham's Nutrient Mixture F-12 (DMEM/F12) supplemented with 7% fetal bovine serum (FBS) and 2.6 mM Lglutamine (complete medium).

M002 Infection, Vaccine Preparation, and IL-12 Assay.
The complete multimodal vaccine (CV-M002) was prepared as follows: Neuro 2a cells were cultured overnight in 24well plates at 6 × 10 5 cells/well followed by incubation (37 ∘ C, 2 hours) with the M002 virus at an MOI of 5. Cells were washed with PBS and resuspended in complete medium and incubated for an additional 30 min. The plate was then irradiated (10 Gy) followed by a medium exchange and additional incubation (37 ∘ C, 24 hours). Control whole cell vaccine (CV) consisted of an identical preparation in which the confluent flask underwent incubation procedures that were the same as M002-infected cells and received the 10 Gy irradiation dose. Supernatants were harvested, clarified by centrifugation, and frozen (−20 ∘ C) for IL-12 analysis.

Murine IL-12 ELISA.
Production of murine IL-12 by the recombinant M002 virus was quantified using a Mouse IL-12 p70 Quantikine ELISA Kit (R&D System, Minneapolis, MN, USA). Twelve well plates were seeded with 2.5 × 10 5 cells per well for 24 hours and then treated as described above (in preparation of cell vaccine). The supernatants were collected at 4, 12, and 24 hr and analyzed by ELISA according to the manufacturer's protocol.

Intracranial Tumor Injection.
Following harvest from log-phase growth in culture, Neuro 2a cells (1 × 10 4 cells) were injected into the right caudate nucleus in 5 L of serumfree DMEM/F12 containing 5% methylcellulose to retard "settling" during injection. This dose was optimized by Parker et al. [2] to define a median survival of 14 to 25 days. Anesthetized mice (ketamine/xylazine) were immobilized using a stereotactic frame. Recovered mice were assessed daily for weight and neurologic impairment and were euthanized on a timed schedule as described below, if they either incurred a 20% weight loss from baseline or appeared moribund. If mice were found dead, death was recorded to end on the previous day.

Monoclonal Antibodies and Flow
Cytometry. Semiquantitative flow cytometric analysis of brain homogenate from mice euthanized at multiple time points after initial tumor implantation was performed as described by Hellums et al. [3]. Beginning at seven days after initial tumor implantation, one mouse was euthanized at 36-48 h intervals. Homogenized cerebrum was enriched for lymphocytes by density-gradient centrifugation. The lymphocyte-rich layer was aspirated, washed, and resuspended in PBS-containing Fc blocker, followed by directly conjugated monoclonal antibodies from the following panel: CD4 (clone GK1.5, Pharmingen), CD8 (clone 53-6.7, Pharmingen), CD3 (clone 17A2), TCR-(clone GL3), and NK cells (NCAM 60). All mAbs were matched in a separate tube by appropriate isotype controls. After incubation (60 min, 4 ∘ C), cells were washed three times and fixed in 1% fresh paraformaldehyde prior to acquisition on an LSR flow cytometer equipped with FACSDiva software (BD Biosciences, San Jose, CA).

Cytotoxicity
Assay. Ten days after the second vaccination with the Neuro 2a vaccine described above, spleens were harvested and splenocytes were isolated by density-gradient centrifugation. Control splenocytes were obtained from naïve mice. Neuro 2a targets were harvested from culture, counted, and labeled with PKH-26 (Sigma-Aldrich, St. Louis, MO, USA) as described by Fischer and Mackensen [14]. Vaccinated splenocytes and naïve splenocytes were incubated with PKH26-labeled Neuro 2a cells at 37 ∘ C for 4 h in effector to target cell ratios of 12.5 : 1, 25 : 1, 50 : 1, and 100 : 1. The vital dye To-Pro-3-Iodide (Molecular Probes/Invitrogen, Carlsbad, CA, USA) was added immediately prior to acquisition on the flow cytometer.

Immunohistochemistry (IHC).
Paraffin embedded sections of mouse brain were postfixed in neutral buffered formalin followed by antigen retrieval with Rodent Decloaker (Biocare Medical, CA, USA). Sections were blocked with avidin and biotin blocks for 20 min (Avidin Biotin Blocking Kit, Biogenex Laboratories) followed by the Rodent Blocker (Bio Care Medical, Richmond, CA, USA) and Fc receptor blocker (Innovex Biosciences) for 30 minutes at RT.
Sections were incubated with rabbit CD3 (Abcam) antibodies overnight at 4 ∘ C following secondary goat antirabbit antibodies (Biocare Medical, Richmond, CA, USA) for 30 min at RT. Sections were next developed with streptavidinlabeled peroxidase and Turbo DAB (Innovex Biosciences, Richmond, CA, USA) for 2-5 minutes and counterstained with hematoxylin.

Data Analysis and Statistical Procedures. Statistical analysis was performed in the UAB Comprehensive Cancer
Biostatistical and Bioinformatics Shared Services Facility. Descriptive statistics were used to express data from cytotoxicity assays. The primary endpoint in this study was

Results
We initially hypothesized that the treatment with N2a tumor cells transduced with HSV1 (M002) encoding murine IL12 will demonstrate enhanced antitumor activity compared to M002 alone (or serve as more efficient vaccination vehicle than virus alone).
To characterize the N2a tumor cell-based vaccine loaded with the virus, we infected cells with M002 at MOI 5 pfu/cell and measured the production of murine IL12 in a culture media at 4, 12, and 24 hrs after infection. The mIL12 concentration in HSV-IL12 infected N2a cells increased in time and reached 3100 pg/mL at 24 hrs ( Figure 1). An irradiation of M002-infected cells was implemented as in vivo required safeguard against the tumor cells proliferation. An irradiation at 10 Gy abolished cell amplification yet, however, still allowed cells to remain metabolically active for a certain time. In infected and irradiated (1 hr after infection) N2a cells, the production of mIL12 followed a similar pattern of the IL12 increase as detected in nonirradiated cells. Importantly, the IL12 levels were comparable with nonirradiated infected cultures. Cellular vaccine presented by irradiated N2a cells loaded with HSV-IL12 was used in our study for peripheral (intramuscular) delivery of the mixture of tumor antigens, conditionally replicative HSV, and murine IL12 in an attempt to favorably change generally insufficient immune response to orthotopically implanted tumors.
Neuro 2a, one of the several clonal derivatives of the C-1300 spontaneous neuroblastoma of A/J mice, has been transplanted in various sites, including intracerebrally, for evaluating multiple therapeutic modalities. Macklis and Madison [15] implanted C-1300 in the brains of mice, and we have modified this approach for this study principally to use survival as our primary indicator. For the in vivo test of vaccine treatment, A/J mice were randomized into three cohorts: no treatment group, treatment with cell vaccine (CV), and treatment with CV (M002), complete multimodal cell vaccine. Vaccination was performed at 3, 7, and 14 days after tumor implantation (Figure 2(a), groups 1-3). In addition, two groups that received M002 viral treatment (groups 4-5) were included for comparison: both received single intracranial injection of virus at day 3, whereas the last group followed a boost with two intramuscular (IM) injections of CV. Contrary to our initial hypothesis, in vivo testing of HSV-IL12 loaded tumor cell vaccine did not result in any therapeutic benefit (increased survival) compared to either untreated group or vaccination with N2a tumor cells alone. In fact, neither the complete vaccine, a combination of irradiated N2a cell vaccine and M002 (CV-M002), nor the N2a cell vaccine alone (CV) conferred a significant survival advantage over untreated controls (Figure 2(b)). Mice that received three intramuscular injections of complete vaccine showed a median survival of 14 days and there was no advantage over 15 days for untreated tumor-bearing controls and 14.5 days for mice that received the N2a cell vaccine only ( = 0.7154 and 0.1261, resp.). There was no advantage for the complete M002 vaccine over the irradiated whole cell vaccine in this model ( = 0.7199). In these experimental settings, only viral treatment administered either as single intracranial injection at day 3 or followed by 2 N2a injections at days 7 and 14 modestly increased median survival time over untreated animals (20 days, 18.5 days, and 15 days correspondingly) ( = 0.0012). However, the combination of M002+radiated N2a cells provides no additional benefit over M002 alone ( = 0.6126).
Intracranial Neuro 2a tumors present an extremely stringent experimental model with low immunogenicity and rapid growth properties. Not surprisingly, both the whole cell vaccinated A/J mice and mice that received the complete M002 vaccine showed no survival advantage over that of tumor-bearing untreated mice as shown in Figure 2(b).
In order to determine whether an effective adaptive antitumor response still could be induced with tumor cell vaccinations, a second experiment was conducted in which vaccination was performed at 7 days prior to tumor implantation followed by a boost at 7 days following tumor implantation (Figure 3(a)). A/J mice were randomized into three groups: the first consisting of tumor-bearing mice that were not vaccinated, a second cohort that received the whole  cell Neuro 2a prime-boost vaccination regimen (CV), and a third that received the prime-boost vaccination with the complete CV-M002 vaccine. In contrast to vaccine treatment outcomes, a premunition prime-boost strategy conferred a survival advantage for vaccinated A/J mice despite rapid growth pattern of Neuro 2a tumors. A significant survival advantage was seen in mice that received the complete CV-M002 vaccine over that of control mice ( = 0.00002) and over that of mice that received the irradiated whole cell vaccine CV ( = 0.04). Survival of mice that received the whole cell vaccine was not significantly different from unvaccinated control mice ( = 0.12) as shown in Figure 3(b).

Surviving CV-M002 Vaccinated Mice Showed a Durable
Immune Response. CV-M002 vaccinated survivors ( = 6) were rechallenged at day 40 with respective orthotropic tumor implantation in the contralateral hemisphere and compared with naïve mice that were implanted with the corresponding tumor as a positive control (Figure 3(c)). CV-M002-vaccinated mice lived significantly longer than control mice ( = 0.0008). Five of six M002 vaccinated mice survived to the experiment endpoint (100 days) (Figure 3(d)).

The CV-M002 Immune Response to the Vaccine Was
Specific. Vaccine specificity was tested by flank injection of the syngeneic H6 hepatoma cells in CV-M002 surviving mice 60 days following the second intracranial Neuro 2a injection (Figure 3(c)). H6 tumor cells grew quickly in every mouse achieving a mass larger than 25 mm 3 between seven and 10 days following the flank injections. Examination of brains from long-term survivors revealed no evidence of Neuro 2a tumor persisting from the second intracranial implantation. These data are consistent with specificity of CV-M002 vaccine against Neuro 2a tumors.   : Increase in CD4 and CD8 LC in mouse brain in groups vaccinated before intracranial injection of Neuro 2a cells. A/J mice were vaccinated in the gastrocnemius with 1 × 10 6 irradiated CV-M002 seven days prior to establishing brain tumors by stereotactically implanting 1 × 10 4 Neuro 2a cells in the right caudate. Mice were serially euthanized, and the whole brain was prepared for flow cytometry analysis. (a) In this ungated acquisition of brain, the prevalence of CD4+ cells peaks between days 14 and 17 (top panel) and then decreases to a minimum by day 35 while a late response of CD8+ T cells is seen at day +31. (b) NK cell infiltration is small and with little change. T cells show significant infiltration at day +17 with the CD3+CD4+ subset tapering at day +24 as discussed above.

Analysis of Tumor-Infiltrating Lymphocyte (TIL) Phenotypes Reveals a Primary Adaptive T Cell Response. CV-M002
mice that received premunition vaccinations were serially sacrificed to evaluate tumor infiltration by immune cells.
Beginning with day +7 after tumor injection and continuing every 3 days ×5, flow cytometric analysis of lymphocyte subsets was performed on brain/tumor-infiltrating lymphocytes at designated time points (Figure 4). One mouse from the nonvaccinated group (not shown) and one from the CV-M002 vaccine group were euthanized, and the brain underwent FACS analysis to evaluate TIL response using antibodies against CD3, CD4, CD8, and NK 1.1. An increase in CD4+ T cells was seen initially followed by a CD8+ T cell increase until reaching a peak at day +38 after tumor injection (Figure 4(a)). Analysis of NK cells and T cell subsets revealed that NK cell infiltration in the brain is small and with little change in time. All studied T cell subsets (CD3, CD4, and CD8) peaked in numbers at day 17 after tumor inoculation (Figure 4(b)). Thus, T cells show significant infiltration at day +17 with the CD4+ subset tapering at day +24. ) was killed at 36-48-hour intervalsto harvest a brain for ICH. Paraffin embedded sections of brain tumors with adjacent normal tissues were stained with CD3 AB to detect lymphocyte infiltration ( Figure 6). The most prominent infiltrates were detected in both virus-injected groups. Some CD3 infiltration was seen in the brain tumors of CV-M002 vaccinated, but not in CV only group; however, it did not result in difference in overall survival for these two groups.

Discussion
Tumor vaccines have been part of clinical trials for many cancers including prostate, renal cell, gastrointestinal stromal,  lung, and breast [16]. These trials are based on promising laboratory studies that often use autologous tumor that has been sonicated, lysed, irradiated, and/or transduced with a gene therapy vector. Vaccines may require antigen-presenting cells (APC) such as dendritic cells (DC) to initiate a cytotoxic T lymphocyte (CTL) response supplemented with adjuvant immune modulators such as IL-12 or GM-CSF. Vaccine protocols often include a boost injection of the vaccine or of immunomodulators to create a durable immune response to tumor-associated antigens. Vaccine trials directed against high-grade brain tumors such as glioblastoma multiforme [17] have incorporated the use of dendritic cells pulsed with tumor lysate [18,19], antibodies to tumor antigen EGFRvIII [20], irradiated tumor injected with immunomodulators [21], and adoptive transfer of immunity of expanded tumor-infiltrating lymphocytes [22]. These trials are based on multiple laboratory models of tumor vaccines that use varied mechanisms in an attempt to induce a specific, durable antitumor immune response.
In this report, we have demonstrated the efficacy of an antitumor vaccine utilizing autologous (in our case, syngeneic) tumor cells infected with an oncolytic HSV-1. We demonstrated durability and specificity of this response by showing resistance to intracranial rechallenge with the same Neuro 2a tumor cell line but susceptibility to the H6 syngeneic murine hepatoma. These findings were consistent with a durable specific immune response against Neuro 2a tumor. In vitro cytotoxicity assays also confirmed that vaccinated splenocytes killed Neuro 2a tumor cells. Serial FACS analysis of whole brain preparations at multiple time points following vaccination also revealed a robust CTL response to the tumor.
We chose oncolytic HSV for our vaccine studies because of its demonstrated ability to generate an antitumor immune response. We specifically selected M002, an HSV expressing-IL-12, to amplify a more robust T cell response to the tumor. Oncolytic HSV has been used as a primary antiglioma agent in G207 and 1716 trials [23,24]. Both viruses are conditionally replicating HSV that were directly injected into glioblastoma tumors. Phase I/II trials of G207 and 1716 have produced some long-term survivors.
The mechanism of immunity in our experiment is currently under investigation. A vaccine composed of a nonreplicating HSV infected tumor that is irradiated and then presented to dendritic cells has been shown to produce a strong T cell response including the stimulation of tumor reactive IFN-secreting T cells and tumor reactive cytotoxic T cells [25]. IL-12 boosts the Th1 immune response and stimulates the production of IFN-and TNF-. This combination of irradiated tumor cells infected with a conditionally replication-competent oncolytic HSV may produce a more robust immune response in the context of the stress/danger model of immunity as originally described by Matzinger [26,27] based on the expression of stress-related antigens. Indeed, malignant and infected cells, including gliomas, express stress-related antigens that are ligands for NKG2D, which functions in the primary immune response of NK and some T cells and is a coreceptor on CD8+ CTL [5][6][7][8]28]. In addition, M002 virus expression of IL-12 in the local tumor environment amplifies the immune response as described by Parker et al. [2].
Finally, it is generally obvious that prime-boost strategies are impractical as a single approach for most malignancies and especially neuroblastoma as it is impossible to accurately predict its development and characterize a population that would benefit from a preventive vaccine. However, a primeboost approach could be an important component of a combination approach in patients with minimal residual ). Beginning at day seven after initial tumor implantation, one mouse from each group was killed at 36-48 h intervals to harvest a brain for ICH. Paraffin embedded sections of brain tumors with adjacent normal brain were stained with anti-CD3 to detect lymphocyte infiltration. The most evident infiltrates were detected in both control and virus-injected groups. Some CD3 infiltration of tumors was seen in CV-M002, not CV only; however, it did not result in difference in overall survival for these two groups. All pictures were taken at 10x magnification. The scale bars in panels correspond to 500 microns.
or undetectable disease provided that the patient's immune function could be maintained at a healthy baseline or augmented by immune modulators [9,10] such as effector cytokines [11], checkpoint inhibitors [12,13], and/or adoptive cell therapy [29]. In summary, we have shown that a tumor vaccine incorporating the M002 IL-12-expressing virus appears to produce a durable, specific immunization against an aggressive intracranial tumor, although vaccination in a minimal disease state has little if any efficacy. These findings strongly suggest that multimodal vaccine therapy could have an adjuvant role in combination with additional immune modulation for the treatment of high-grade neural tumors.