Journal of Neurodegenerative DiseasesVolume 2013 (2013), Article ID 679089, 4 pageshttp://dx.doi.org/10.1155/2013/679089

Clinical Study

Cerebrospinal Fluid Biomarkers for Kii Amyotrophic Lateral Sclerosis/Parkinsonism-Dementia Complex

Yui Nakayama,¹ Satoru Morimoto,² Misao Yoneda,³ Shigeki Kuzuhara,³ and Yasumasa Kokubo⁴

¹Medical Department, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-8507, Japan²Department of Neurology, Tokyo Metropolitan Geriatric Hospital, 35-2 Sakaemachi, Itabashi-ku, Tokyo 173-0015, Japan³Department of Medical Welfare, Suzuka University of Medical Science, 1001-1 Kishioka-cho, Suzuka City, Mie 510-0293, Japan⁴Department of Neurology, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-8507, Japan

Received 16 January 2013; Accepted 6 March 2013

Academic Editor: Peter Crouch

Copyright © 2013 Yui Nakayama et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract.

Objective. Amyotrophic lateral sclerosis/parkinsonism-dementia complex is classified as one of the tauopathies. Methods. The total tau, phosphorylated tau, and amyloid β42 levels were assayed in cerebrospinal fluid from patients with Kii amyotrophic lateral sclerosis/parkinsonism-dementia complex (), Alzheimer’s disease (), Parkinson’s disease (), amyotrophic lateral sclerosis (), and controls () using specific enzyme-linked immunosorbent assay methods. Results. Total tau and phosphorylated tau did not increase and amyloid β42 was relatively reduced in Kii amyotrophic lateral sclerosis/parkinsonism-dementia complex. Relatively reduced amyloid β42 might discriminate Kii amyotrophic lateral sclerosis/parkinsonism-dementia complex from amyotrophic lateral sclerosis and Parkinson’s disease, and the ratios of phosphorylated-tau to amyloid β42 could discriminate Kii amyotrophic lateral sclerosis/parkinsonism-dementia complex from Alzheimer’s disease. Conclusions. Cerebrospinal fluid analysis may be useful to differentiate amyotrophic lateral sclerosis/parkinsonism-dementia complex from Alzheimer’s disease, amyotrophic lateral sclerosis, and Parkinson’s disease.

1. Introduction

Amyotrophic lateral sclerosis/parkinsonism-dementia complex (ALS/PDC) is a rare disorder endemic to Guam Island and the Kii Peninsula of Japan. It shows a unique combination of parkinsonism, amyotrophy, and dementia [1], and the form of dementia, which shows a phenotype similar to Alzheimer’s disease (AD), is becoming predominant in the Kii Peninsula.

Although Kii ALS/PDC shows several unique clinical features, including severe atrophy of the frontal and temporal lobes on magnetic resonance imaging (MRI), decreased cerebral blood flow in the frontal and temporal lobes on single-photon emission computed tomography (SPECT) [2], pigmentary retinopathy [3], and decreased cardiac ¹²³I-meta-iodobenzylguanidine uptake [4], a postmortem examination is required for a definitive diagnosis. Since biomarkers for ALS/PDC have not yet been identified, we analyzed cerebrospinal fluid (CSF) biomarkers for Kii ALS/PDC to discriminate it from other neurodegenerative disorders.

2. Material and Methods

We collected CSF samples from 12 patients with Kii ALS/PDC (6 men, 6 women, mean age years, mean illness duration 5.63 years), nine patients with AD (2 men, 7 women, mean age years, mean illness duration 1.92 years), 11 patients with ALS (8 men, 3 women, mean age years, mean illness duration 1.1 years), nine patients with Parkinson’s disease (PD; 7 men, 2 women, mean age years, mean illness duration 4.42 years), and five disease control patients (C; 4 men, 1 woman, mean age years). All of the patients with Kii ALS/PDC were natives of Hohara village, which is an area of high ALS/PDC prevalence on the Kii Peninsula. We collected CSF samples over 10 years; therefore, the period between CSF collection and analysis was not standardized. The diagnosis of Kii ALS was made according to the Airlie House criteria, since the clinical symptoms of Kii ALS are essentially the same as those of classical ALS. The diagnosis of Kii PDC was made by a unique combination of levodopa-unresponsive parkinsonism and dementia, which are frequently accompanied by amyotrophy of the extremities and/or pyramidal tract signs. Mini-mental state examination (MMSE) was used for the evaluation of dementia and cut-off point was 23 (data not shown). The frontal lobes and/or temporal lobes of ALS/PDC patients showed atrophy on MRI and/or a decrease of cerebral blood flow on SPECT. CSF samples were immediately centrifuged at 1000 ×g for 15 min and stored at −80°C with polypropylene tube. The total tau (t-tau), phosphorylated tau (p-tau), and amyloid beta (A) concentrations were measured with an enzyme-linked immunosorbent assay (ELISA) kit using a monoclonal antibody specific for t-tau, p-tau, and A1–42 (INNOTEST hTAU Ag, phosphor tau(181P), and -amyloid (1–42), Innogenetics, Ghent, Belgium). ELISA assays were carried out using several samples from each group on the same plate in a randomized manner and were repeated using randomized samples in the same manner in plural times. A factorial ANOVA was performed with CSF-t-tau, CSF-p-tau, and CSF-A42, as dependent variables, with the diagnostic category (AD, ALS, C, Kii ALS/PDC, and PD) using JMP 9.0. All data were expressed as means ± SD. A value less than 0.05 was considered statistically significant. The Ethics Committee of Mie University Graduate School of Medicine approved this study and the “Declaration of Helsinki” was followed. Informed consent was obtained from the patients or their families.

3. Results

CSF-A42, CSF-t-tau, and CSF-p-tau were compared between AD, ALS, C, Kii ALS/PDC, and PD. The concentrations of CSF-t-tau and CSF-p-tau were significantly higher in AD (t-tau; 378.0 ± 41.76 pg/mL; , p-tau; 42.4 ± 6.78 pg/mL; ) than in the other groups. However, the concentrations of CSF-t-tau and CSF-p-tau did not differ significantly between Kii ALS/PDC, ALS, C, and PD (Figures 1(a) and 1(b)). The concentration of CSF-A42 was significantly reduced in AD ( pg/mL; ) compared to ALS and C and relatively reduced in Kii ALS/PDC (465.4 ± 53.69 pg/mL; ) compared to ALS. Most of the ALS/PDC patients had CSF concentration values that fell below the cutoff based on C (Figure 1(c)). The ratios of CSF-p-tau to CSF-Aβ42 were significantly increased in AD () compared with Kii ALS/PDC (; ), ALS (; ), PD (; ), and C (; ) (Figure 1(d)). The concentrations of CSF-t-tau, CSF-p-tau, or CSF-Aβ42 were not related to the clinical parameters (age, sex, illness duration, or dementia) in the Kii ALS/PDC patients. The number of C samples was small and the average age of control patients was low.

(a)

(b)

(c)

(d)

Figure 1: Analysis of CSF biomarkers. (a) Total tau, (b) phosphorylated tau, (c) beta-amyloid peptide (1–42), (d) the ratio of p-tau to A42. AD: Alzheimer’s disease, ALS: amyotrophic lateral sclerosis, C: disease control, Kii: Kii amyotrophic lateral sclerosis/parkinsonism-dementia complex, and PD: Parkinson’s disease.

Generally, CSF tau level gradually increase according to age and CSF A is not affected by age [5]. CSF tau level of C samples was relatively low, but it was not significant.

Thus, the CSF values of C were not comparable to those of other groups. Nevertheless, the optimal cut-off values that discriminate C from AD were similar to those in previous reports [6, 7] in which a larger number of control samples were analyzed. CSF tau level of Kii ALS/PDC samples did not increase, although the average age of Kii ALS/PDC group was older than that of AD group.

4. Discussion

The present study showed that CSF-t-tau and CSF-p-tau concentrations from patients with Kii ALS/PDC were not increased compared to those in the other disease groups, and Aβ42 concentration in the CSF was relatively decreased. The ratio of CSF-p-tau to CSF-Aβ42 segregates Kii ALS/PDC from AD. Because ALS/PDC is associated with tau pathology in the absence of amyloid plaques, the expectation was that ALS/PDC patients would not show the Alzheimer’s disease (AD) profile of decreased A42 but might show increased t-tau and/or p-tau in the CSF.

In general, decreased CSF-Aβ42 indicates plaque pathology, and increased CSF-t-tau and CSF-p-tau indicate axonal degeneration and tangle pathology, respectively [8]. Recently, the average age of onset of Kii ALS/PDC is increasing and Aβ deposition is conspicuous in autopsied patients. Therefore, decreased CSF-Aβ42 may reflect Aβ pathology in the most recent patients. We analyzed the precise tau isoform of over 10 patients with autopsy-proven ALS/PDC recently and identified a 3R + 4R type, type, and a 4R predominant type. The glial tau pathology is particularly related to the 4R isoform, and we consider Kii ALS/PDC to be a 4R-dominant tauopathy (unpublished data). Noguchi et al. examined the concentrations of CSF-t-tau, CSF-p-tau, and CSF-Aβ42 in patients with progressive supranuclear palsy (PSP) and corticobasal degeneration (CBD); the concentrations of CSF-t-tau and CSF-p-tau did not significantly differ between PSP, CBD, and controls, and the concentration of CSF-Aβ42 was significantly lower in PSP and CBD than in controls. The authors speculated that the absence of an increase of CSF-t-tau and CSF-p-tau concentrations might reflect 4R tau predominance and a reduction of CSF-Aβ42 might suggest deposition or mismetabolism of Aβ [6]. Taken together, CSF biomarkers of Kii ALS/PDC might have similar properties to those of 4R tauopathy, PSP, and CBD; however the relationship between tau isoform and CSF tau level remains to be resolved.

Finally, the present findings, in which CSF-t-tau and CSF-p-tau concentrations were not increased and CSF-Aβ42 concentration was relatively decreased, suggest that CSF analysis may be useful to differentiate ALS/PDC from AD, ALS, and PD. Nevertheless there is a major limitation of the interpretation of the data. The size of each group is small, the age of the control group is much younger, and there were two populations in the AD group regarding the levels tau, p-tau, and Aβ/p-tau. Further study using groups with larger size of subjects is needed to confirm the proposed utility of the CSF biomarkers.

Abbreviations

ALS/PDC: | Amyotrophic lateral sclerosis/parkinsonism-dementia complex
CSF: | Cerebrospinal fluid
NFTs: | Neurofibrillary tangles
t-tau: | Total tau
p-tau: | Phosphorylated tau
AD: | Alzheimer’s disease
PD: | Parkinson’s disease
MRI: | Magnetic resonance imaging
SPECT: | Single-photon emission computed tomography
C: | Control patients
ELISA: | Enzyme-linked immunosorbent assay
3R: | 3 repeat
4R: | 4 repeat
PSP: | Progressive supranuclear palsy
CBD: | Corticobasal degeneration.

Disclosure

Y. Kokubo certifies that all my affiliations or financial involvement, within the past year and in the foreseeable future (e.g., employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, and royalties) with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the paper are completely disclosed. All authors report no disclosures and no conflicts of interest concerning the research related to the paper. There was no ghost writing by anyone not named in the authors list.

Funding

This study was partly supported by a Grant-in-Aid from the Nagao Memorial Fund and the Mie Medical Fund; a Grant-in-Aid from the Research Committee of CNS Degenerative Diseases; a Grant-in-Aid from the Research Committee of Muro Disease (Kii ALS/PDC) (to Y. Kokubo: 21210301), the Ministry of Health, Labor, and Welfare, Japan; and a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports, and Culture, Japan. The funding sources had no involvement in the study design or in the collection, analysis, and interpretation of data or in the writing of the paper or the decision to submit the paper for publication.

Acknowledgments

The authors thank Ms. Hisami Akatsuka and Ms. Jyunko Karita for their technical assistance in preparing CSF samples for ELISA examination. Registration number of UMIN clinical trials registry is R000011521.

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