Journal of Nanomaterials

Volume 2014 (2014), Article ID 257391, 8 pages

http://dx.doi.org/10.1155/2014/257391

Research Article

The Long-Term Fate and Toxicity of PEG-Modified Single-Walled Carbon Nanotube Isoliquiritigenin Delivery Vehicles in Rats

Bo Han,1,2 Mingming Zhang,¹ Ting Tang,³ Qiusheng Zheng,1,2 Keyu Wang,¹ Le Li,1,2 and Wen Chen1,2

¹Pharmacy School of Shihezi University, Shihezi 832002, China

²Key Laboratory of Xinjiang Phytomedicine Resources, Shihezi 832002, China

³The Ankang Hospital of Xinjiang P&C Corps, Shihezi 832002, China

Received 28 October 2013; Revised 11 December 2013; Accepted 12 December 2013; Published 16 March 2014

Academic Editor: Jinlong Jiang

Copyright © 2014 Bo Han et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract.

Oxidized single-walled carbon nanotubes (o-SWNTs) was modified by covalently and noncovalently linking PEG to the o-SWNTs. The influence of oxidation time, PEG molecular weight, and type of PEG linkage on the blood clearance time of PEG-modified single-walled carbon nanotubes (SWNTs) was investigated. The toxicity profile of SWNTs covalently linked to PEG (c-PEG-o-SWNTs) in rats has also been determined. The pharmacokinetics of c-PEG-o-SWNTs in rats and their distribution in vital organs were monitored by Raman spectroscopy, and the blood clearance of homogenate isoliquiritigenin (ISL) was determined by HPLC. Photos of tissue and tissue sections were taken to evaluate the toxicity of c-PEG-o-SWNTs. We found that SWNTs which were covalently modified with PEG and have a molecular weight of 3500 had the longest blood clearance half-lives. However, SWNTs were toxic to the kidneys and the hearts. The high renal clearance of long-term fate SWNTs may occur because of impaired kidney filtration function. Therefore, we assume that while researchers study the long-term fate of SWNTs, the toxicity of SWNTs also needs to be taken into account.

1. Introduction

The root of Glycyrrhiza uralensis  Fischer is widely used as a harmonizing ingredient in traditional herbal formulations in oriental medicine; it is used in more formulations than any other herb. One of the most notable ingredients in Glycyrrhiza uralensis  Fischer is isoliquiritigenin (ISL), a simple chalcone-type flavonoid that has antioxidative effect [1, 2], antiplatelet aggregation effect [3], anti-ischemia effect [4], anti-inflammatory property [5, 6], antispasmodic effect [7], and estrogenic property [8]. In the past seven years, our group has expanded the study of ISL drug performance [9–11] and has made progress in ISL tumor pharmacology [12], natural product chemistry [11], and in vivo analysis [13]. In this study, we aim to improve the target performance of ISL by delivering the compound with the help of SWNTs.

SWNTs have been heavily studied because of their unique physicochemical properties and potential applications [14]. Encouraged by the success of using carbon nanotubes (CNTs) for in vitro sensing, drug delivery, and imaging, researchers in this field have moved to in vivo investigations in animals [15].

In vivo biodistribution andpharmacokinetic studies have been carried out bya number of groups using different CNT materials. Many of these studies confirmed the poor biocompatibility of original carbon nanotube formulations [16]. Purified SWNTs implanted in mice via intratracheal instillation resulted in epithelioid granulomas and interstitial inflammation in a 90-day study [17]. Lam et al. found that undoped multiwalled CNTs (MWCNTs) can induce severe granulomatous inflammatory responses compared with nitrogen-doped MWCNTs when administered intratracheally in mice [18]. Original MWCNTs inhaled by mice for 6 h were found throughout the lungs and significant pulmonary toxicity was observed [19].

SWNTs used for drug delivery are usually functionalized with polyethylene glycol (PEG). PEG provides a desirable coating for nanotubes and nanoparticles, because it reduces their immunogenicity and reduces their chance of being nonspecifically taken up by cells of the reticuloendothelial system. Consequently, the phagocytosis of nanoparticles is diminished and their clearance time is prolonged when modified with PEG [20].

The purpose of our study is to investigate the influence of SWNTs’ oxidation time, PEG molecular weight, and PEG linkage type on the clearance time of SWNTs. After the best long-term fate single-walled carbon nanotubes (LTFS) were synthesized, the blood clearance time changes between ISL and ISL loaded with LTFS were investigated. The toxicity of LTFS in rats was also studied. While there are many researchers concern about the toxicity of carbon nanotube as well as its long-term fate, there is little work focusing on the relationship between the toxicity and the long-term fate of CNTs.

2. Materials and Methods

2.1. Reagents, Instruments, and Test Animals

2.1.1. Reagents

SWNTs were obtained from Nanjing Jicang Technology Co., Ltd ; ISL was purchased from Tianye Chemical Co., Ltd. ; N-Hydroxysuccinimide (NHS) and carbodiimideHCl were obtained from GL Biochem (Shanghai), Ltd.; NH₂-PEG-NH₂ (MWs of 2000, 3500, and 5000) were purchased from Beijing Kaizheng Biotech Development Co., Ltd. Heparin sodium injection was purchased from Tianjin Biochemical Co., Ltd.; mixed phosphate buffer solution (PBS) was purchased from Sigma-Aldrich; and nitric acid, sulfuric acid, ethyl acetate, dimethyl sulfoxide, and anhydrous ethanol (A.R.) were purchased from Sinopharm Chemical Reagent Co., Ltd.

2.1.2. Instruments

An STA409PC composite thermal analyzer and Al₂O₃ crucible were used in this study (NETZSCH Scientific Instrument Trading (Shanghai) Co., Ltd.). An H-600 transmission electron microscope (Japan Electronics Co., Ltd.); a Raman spectrometer (Bruker 70), a dialysis bag (MWCO 7000–14000; Hainan Nanjing Science and Technology Development Co., Ltd.), an LC-20A high-performance liquid chromatograph (Shimadzu); and a chromatographic column were also used.

2.1.3. Test Animals

Six-week-old Wistar rats with body weight of and production certificate number of SCXK (Xin) (Animal Center of Xinjiang Medical University) were used in this study.

2.2. Preparation of PEGylated Nanotubes

One hundred mg of HiPco SWNTs were added to 50 mL of a 1 : 1 HNO₃-H₂SO₄ solution. The mixture was refluxed for 15 min, 30 min, or 45 min at 140°C, respectively, and then cooled. The mixture was diluted with 250 mL of deionized water and filtered through a microporous membrane. The filter film containing oxidized SWNTs (o-SWNTs) was then washed with deionized water. The carboxyl groups present on o-SWNTs surfaces provide the binding sites required for attaching PEG.

Covalently linked PEG to o-SWNTs (c-PEG-o-SWNTs) was prepared according to the following steps. 50 mg of o-SWNTs was combined with 50.00 mL of phosphate buffer (pH 6.8) under sonication to achieve an evenly dispersed solution. 200 mg carbodiimide (EDC) and 250 mg NHS were added into the dispersed solution and then the mixture was sonicated for 30 min. After that, 50 mg NH₂-PEG-NH₂ (MW of 2000, 3500, or 5000) was added and the mixture was magnetically stirred at room temperature for 24 h. Distilled water was added to achieve a final volume 3 times larger than initial volume. The diluted mixture was centrifuged at 22 000 g for 1 h. The supernatant was placed in a dialysis bag dialysed to neutral pH [21]. The amount of grafted PEG was determined by the thermal gravimetric analysis method (TG) [22].

Noncovalently linked PEG to o-SWNTs (n-PEG-o-SWNTs) was prepared according to the following steps. 50 mg of o-SWNTs was combined with 50.00 mL of pH 6.8 phosphate buffer solution and then sonicated for 30 min. The solution obtained was combined with 50 mg NH₂-PEG-NH₂ (MW 2000) and stirred evenly at room temperature for 24 h to get n-PEG-o-SWNTs [21]. The amount of grafted PEG was determined by TG [22].

2.3. Preparation of ISL and Functionalization of SWNTs Complexes

In order to attach ISL onto c-PEG-o-SWNTs noncovalently, 12 mg of ISL was dissolved in a mixture of DMSO, PEG400, and saline (1 : 2 : 9) to make a 1 mg/mL ISL solution. 60 mg of c-PEG-o-SWNTs was added to the ISL solution and the mixture was stirred for 6 h in a 25°C water bath to obtain SWNT-ISL complexes (c-PEG-o-SWNTs-ISL). The prepared products were resuspended and stored at 4°C.

2.4. Animal Experiments

Twenty-four hours after injecting 200 μL of the c-PEG-o-SWNTs-ISL test solution into rats’ tail veins, 0.5 mL of blood was obtained through orbital veins and placed into heparin sodium treated centrifuge tubes. After centrifuging the blood for 10 min at 5000 rpm, 200 μL plasma was collected for future use. Then rats were killed by cervical dislocation, and their hearts, livers, spleens, lungs, kidneys, and brains were collected and weighed. The various tissues and organs were placed in 4 mL centrifuge tubes containing 2 mL of normal saline and homogenized. Two portions of homogenate (7.5 mL) were taken. One portion was used to determine the ISL content by HPLC. The other portion was combined with 0.75 mL of CMC-Na solution and then homogenized by vortex oscillation for 5 min. This portion was reserved for Raman spectroscopy.

2.5. Raman Measurement of PEG-SWNT Solutions

Various concentrations of SWNT solutions were drawn into capillary glass tubes (Fisher) and measured using a Bruker 70 Raman spectrometer (laser excitation wavelength was 1024 nm; 30 min; 850 times). Four spectra were measured for each sample to obtain an average spectrum. For a given concentration of SWNTs, the Raman intensity was obtained by integrating the PEG-o-SWNT G-band peak area from 1570 cm⁻¹ to 1620 cm⁻¹. The Raman G-band peak areas were used to calculate PEG-o-SWNT concentrations in the blood. The percentage of injected dose per gram of blood was calculated using the following equation:

2.6. Determination of Isoliquiritigenin Content in the Tissue Homogenate with HPLC

The mobile phase consisted of a mixture of acetonitrile and double distilled water (1 : 1 (v/v), the flow rate was 10 mL/min, and the column temperature was 40°C. A sample volume of 20 μL was injected into the HPLC. The ISL detection wavelength was 368 nm and the operating time was 9 min.

2.7. Statistical Analysis

The pharmacokinetic parameters were performed by DAS (ver1.0) program and compared by statistical analysis. One-way ANOVA was used for parametric data, when differences were statistically significant .

3. Results and Discussion

3.1. Characterization of PEG-Modified SWNTs

The structure of c-PEG-o-SWNTs after synthesized by EDC is shown in Figure 1. SWNTs covalently functionalized by PEG can form stable solution in water [23]. Centrifugation was used to remove big bundles and impurities, leaving short individual and small bundles of tubes in solution. Strong resonance Raman scattering is an intrinsic optical property of SWNTs, with sharp peaks and low background in the spectra. The tangential graphite-like phonon mode (G band), which is the strongest peak in the SWNTs Raman spectrum, was used to detect nanotubes in blood and tissue lysates.

Figure 1: The structure of c-PEG-o-SWNTs.

3.2. Determination of c-PEG-o-SWNTs In Vivo by Raman Spectroscopy

SWNTs have a unique crystal structure, which produces unique Raman scattering at a wavelength of about 1590 nm. SWNTs retain the feature of Raman scattering after covalent or noncovalent modification with PEG. Figure 2 shows the Raman spectra of different concentrations of c-PEG-o-SWNTs in plasma solutions. The G bands of SWNTs were absorbed, and the absorption strength increased as the c-PEG-o-SWNT concentration in solution increased. With the above positive correlation, c-PEG-o-SWNTs in the blood and tissue fluid were quantified by using the peak area of the G band. The data were fitted by the nonlinear standard curve using Origin 7.5 software. The results of the sampling recovery test, precision test, and durability test showed that the amount of c-PEG-o-SWNTs in the animals’ tissues and blood were accurately determined by using this method.

Figure 2: The Raman spectra of different concentrations of c-PEG-o-SWNTs in blood samples.

3.3. Determination of Isoliquiritigenin Content in Rat Tissues by HPLC

The ultraviolet absorption spectrum of ISL with a maximum absorption wavelength of 368 nm. ISL was eluted at 5.1 min under the HPLC conditions employed in this work, while blank plasma revealed no peak at or around 5.1 min under the same conditions, suggesting the good specificity of determining ISL by HPLC. The results of the sampling recovery test, precision test, and durability test displayed that the ISL content in the animals’ tissues and blood were accurately assayed by this method [11].

3.4. Investigation on Factors That Influence the Blood Clearance Time of SWNTs

Figure 3(a) shows the influence of different types of PEG modification (covalent, noncovalent, or no PEG modification) on the blood clearance time of SWNTs. The half-lives of SWNTs were , , and . Covalently linking NH₂-PEG-NH₂ (MW 2000) significantly prolonged the clearance time of SWNTs, but noncovalently linking NH₂-PEG-NH₂ (MW 2000) to SWNTs did not. These results imply that noncovalent modification of SWNTs using NH₂-PEG-NH₂ (MW 2000) may not protect SWNTs from clearance by the reticuloendothelial system. In contrast, covalently linking NH₂-PEG-NH₂ (MW 2000) increases the blood clearance time of SWNTs.

(a)

(b)

(c)

Figure 3: The investigation of the influence of different types of PEG modification factors (a), different degrees of oxidation on SWNT clearance time (b), and the influence modifying SWNTs with different PEG molecular weights (c) on the SWNT clearance time in vivo.

Figure 3(b) shows the influence of different degrees of oxidation on SWNT clearance time. Using TG calculation, the carboxyl contents were found to be 9.44%, 13.18%, and 15.32% at 15 min, 30 min, and 45 min of oxidation, respectively. Through the DAS 1.0 pharmacokinetic software calculation, we found that CNTs covalently linked to NH₂-PEG-NH₂ (MW 2000) caused blood clearance half-lives of 1.57 h, 2.35 h, and 1.61 h according to oxidation time of 15 min, 30 min, and 45 min, respectively. These results suggest that 30 min of mixed acid oxidation results in SWNTs that have the longest blood clearance time. Oxidation time longer than 30 min did not extend the clearance time of SWNTs.

Figure 3(c) shows the influence of modifying SWNTs with different PEG molecular weights on the SWNT clearance time. Through calculation, the half-lives were , , . Although PEG modification on the surface of SWNTs prolonged their blood clearance time, the molecular weight of NH₂-PEG-NH₂ that can prolong blood clearance time to the highest level remains unclear.

In summary, these results suggest that 30 min of mixed acid oxidation of SWNTs followed by covalently linking NH₂-PEG-NH₂ (MW 3500) resulted in SWNTs with the longest blood clearance time. After the formulation of the best LTFS was obtained, ISL was incorporated into the LTFS (LTFS-ISL), and the pharmacokinetic profile and the toxicity of LTFS-ISL on rats were determined.

The research group led by Dai et al. at Stanford University found that ultrasonic dispersion of multiwalled carbon nanotubes with a molecular weight of 7000 for 1 h resulted in a long-term fate of one day after tail vein injection [16]. However, the Hudson group at the University of New Mexico has contended that one hour of ultrasonic dispersion will break the long chain of PEG and thereby reduce the actual molecular weight of the PEG on the carbon nanotubes [24]. In this work, we did not use ultrasonic-assisted dispersion of c-PEG-o-SWNTs, and our results suggest that SWNTs covalently linked to PEG with a molecular weight of 3500 display the longest blood clearance half-live.

3.5. Influence of LTFS on ISL Blood Clearance Time and Distribution

Figure 4 shows the concentration of ISL in blood with or without the SWNT carriers. Using the DAS 1.0 pharmacokinetic software, we determined that , demonstrating that the existence of carriers reduces the half-life of ISL in the blood.

Figure 4: The blood concentration of ISL with and without LTFS. The y-axis represents injected dose per gram .

Interestingly, as shown in Figure 4, the existence of carriers significantly reduces the concentration of ISL in blood. Thirty minutes after LTFS-ISL was injected through tail veins, the rats developed gross hematuria and black discharge in the urine, raising the possibility that LTFS-ISL may cause renal toxicity and that carbon nanotubes were discharged through the urine.

3.6. Appearance Changes in the Heart and Kidneys after the Injection of LTFS-ISL

We compared the hearts, livers, spleens, lungs, and kidneys of rats injected with ISL and LTFS-ISL. The results showed that the hearts and kidneys of rats in the LTFS-ISL group were blacker and larger than those of rats in the ISL group, suggesting that the carbon nanotubes remained in the hearts and kidneys, as shown in Figure 5.

Figure 5: The influence of ISL and LTFS-ISL on the appearance of various organs.

3.7. Tissue Distribution of c-PEG-3.5 k-o-SWNTs-30 min

We determined the content of carbon nanotubes in the tissues (hearts, livers, spleens, and kidneys) and in the urine of rats injected with LTFS using Raman spectroscopy. Figure 6 shows the content of LTFS in the tissues and urine. It was discovered that the content of LTFS in the urine, liver, spleen, and kidney was higher than that in other organs. The results suggest that, like most nanosubstances, macrophages readily consume LTFS in the liver, spleen, and other organs through phagocytosis.

Figure 6: The distribution of LTFS in the tissues and urine. “*” indicates the differences were statistically significant compared with urine bar .

It should be noted that the discharged urine contained a large amount of carbon nanotubes, which is consistent with reports by Singh et al. [25] and Lacerda et al. [26], indicating that most carbon nanotubes are removed through kidney. From our experimental results and the work presented by Singh and Lacerda, we speculate that ISL can be adsorbed onto the surface of LTFS. Therefore, after LTFS are removed by kidney and the reticuloendothelial system, the concentration of ISL in blood significantly decreases.

3.8. Histopathology Changes after Injecting LTFS-ISL in the Heart and Kidney Tissues

We examined the heart and kidney tissue sections after H&E staining. As illustrated in Figure 7, the myocardial cell cytoplasm of LTFS-ISL-treated rats changed into granules and vacuoles in some regions, and the color of the myocardial fibers faded and the nuclei of myocytes shrank. In addition, there were vacuolar deformations and particulate deformations in kidney tubules after LTFS-ISL administration. The above results suggest that LTFS-ISL may cause heart and kidney toxicity. The large discharge of LTFS-ISL in urine may be attributed to glomerular damage caused by LTFS-ISL.

Figure 7: The effect of LTFS-ISL on the heart and kidneys. The arrows indicate that the carbon nanotubes remained in some organs. The scale bar is 100 μm.

4. Conclusions

We speculate that LTFS-ISL is toxic to kidney and heart. The results of our study showed that the longest half-life of PEG-o-SWNTs was obtained after SWNTs underwent reflux oxidation for 30 min and were covalently linked with NH₂-PEG-NH₂ (MW 3500). These results indicate that surface modification of SWNTs with NH₂-PEG-NH₂ indeed prolongs their clearance time, and c-PEG-o-SWNT carriers reduce the blood clearance half-life of ISL. The black and red discharge in urine and postmortem tissue sections infer that LTFS are likely to cause liver and kidney toxicity. The increased renal clearance of LTFS may occur owing to impaired kidney filtration function. This discovery provides a new way to explain of long-term fate SWNTs’ high renal clearance in rats.

Conflict of Interests

The authors declare that they have no conflict of interests.

Acknowledgment

This work was supported by the National Natural Science Foundation of China (nos. 30960515 and 81260487), the Doctor Foundation of Xinjiang Bingtuan (no. 2012BB020) and the Foundation from Shihezi University (ZRKXYB-13, YX07010, RCZX201115).

References

T. Umemura, R. Kitaguchi, K. Inagaki, and H. Haraguchi, “Direct injection determination of theophylline and caffeine in blood serum by high-performance liquid chromatography using an ODS column coated with a zwitterionic bile acid derivative,” Analyst, vol. 123, no. 8, pp. 1767–1770, 1998.

H.-X. Liu, W.-H. Lin, X.-L. Wang, and J.-S. Yang, “Flavonoids from preparation of traditional Chinese medicines named Sini-Tang,” Journal of Asian Natural Products Research, vol. 7, no. 2, pp. 139–143, 2005.

M. Tawata, K. Aida, T. Noguchi et al., “Anti-platelet action of isoliquiritigenin, an aldose reductase inhibitor in licorice,” European Journal of Pharmacology, vol. 212, no. 1, pp. 87–92, 1992.

C. Zhan and J. Yang, “Protective effects of isoliquiritigenin in transient middle cerebral artery occlusion-induced focal cerebral ischemia in rats,” Pharmacological Research, vol. 53, no. 3, pp. 303–309, 2006.

J.-Y. Kim, S. J. Park, K.-J. Yun, Y.-W. Cho, H.-J. Park, and K.-T. Lee, “Isoliquiritigenin isolated from the roots of Glycyrrhiza uralensis inhibits LPS-induced iNOS and COX-2 expression via the attenuation of NF-κB in RAW 264.7 macrophages,” European Journal of Pharmacology, vol. 584, no. 1, pp. 175–184, 2008.

D.-C. Kim, S.-Y. Choi, S.-H. Kim et al., “Isoliquiritigenin selectively inhibits H₂ histamine receptor signaling,” Molecular Pharmacology, vol. 70, no. 2, pp. 493–500, 2006.

Y. Sato, J.-X. He, H. Nagai, T. Tani, and T. Akao, “Isoliquiritigenin, one of the antispasmodic principles of Glycyrrhiza ularensis roots, acts in the lower part of intestine,” Biological and Pharmaceutical Bulletin, vol. 30, no. 1, pp. 145–149, 2007.

S. Tamir, M. Eizenberg, D. Somjen, S. Izrael, and J. Vaya, “Estrogen-like activity of glabrene and other constituents isolated from licorice root,” Journal of Steroid Biochemistry and Molecular Biology, vol. 78, no. 3, pp. 291–298, 2001.

B. Han, Q.-N. Li, S.-W. Wu, J.-G. Li, W. Chen, and W.-X. Li, “Selective adsorption of multi-walled carbon nanotubes with liquiritin and isoliquiritin,” Yao Xue Xue Bao, vol. 42, no. 11, pp. 1222–1226, 2007.

B. Han, W. Chen, W.-J. Jin, and S.-P. Liu, “Evaluation of AB-8 macroporous adsorption resin for adsorption of total flavones in liquorice,” Nan Fang Yi Ke Da Xue Xue Bao, vol. 27, no. 3, pp. 265–267, 2007.

B. Han, Q. -s. Zheng, W. Chen, X. Wang, Q. Wang, and L. Li, “A novel process for extraction of isoliquiritigenin from licorice (Glycyrrhiza glabra) roots by facile convert,” Chemistry of Nature Compounds, vol. 46, no. 4, pp. 523–527, 2010.

D. Li, Z. Wang, H. Chen et al., “Isoliquiritigenin induces monocytic differentiation of HL-60 cells,” Free Radical Biology and Medicine, vol. 46, no. 6, pp. 731–736, 2009.

B. Han, W. Chen, Q. Zheng et al., “Determination of isoliquiritigenin and its distribution in mice by synchronous fluorescence spectrometry,” Analytical Sciences, vol. 27, no. 11, pp. 1115–1119, 2011.

S. A. Hodge, M. K. Bayazit, K. S. Coleman, and M. S. Shaffer, “Unweaving the rainbow: a review of the relationship between single-walled carbon nanotube molecular structures and their chemical reactivity,” Chemical Society Reviews, vol. 41, no. 12, pp. 4409–4429, 2012.

H. Ali-Boucetta and K. Kostarelos, “Carbon nanotubes in medicine and biology: therapy and diagnostics,” Advanced Drug Delivery Reviews, vol. 65, no. 15, pp. 1897–1898, 2013.

Z. Liu, S. Tabakman, K. Welsher, and H. Dai, “Carbon nanotubes in biology and medicine: In vitro and in vivo detection, imaging and drug delivery,” Nano Research, vol. 2, no. 2, pp. 85–120, 2009.

C.-W. Lam, J. T. James, R. McCluskey, and R. L. Hunter, “Pulmonary toxicity of single-wall carbon nanotubes in mice 7 and 90 days after intractracheal instillation,” Toxicological Sciences, vol. 77, no. 1, pp. 126–134, 2004.

J. C. Carrero-Sánchez, A. L. Elías, R. Mancilla et al., “Biocompatibility and toxicological studies of carbon nanotubes doped with nitrogen,” Nano Letters, vol. 6, no. 8, pp. 1609–1616, 2006.

K. Donaldson and C. A. Poland, “Nanotoxicology: new insights into nanotubes,” Nature Nanotechnology, vol. 4, no. 11, pp. 708–710, 2009.

S. M. Ryan, G. Mantovani, X. Wang, D. M. Haddleton, and D. J. Brayden, “Advances in PEGylation of important biotech molecules: delivery aspects,” Expert Opinion on Drug Delivery, vol. 5, no. 4, pp. 371–383, 2008.

B. Zhao, H. Hu, A. Yu, D. Perea, and R. C. Haddon, “Synthesis and characterization of water soluble single-walled carbon nanotube graft copolymers,” Journal of the American Chemical Society, vol. 127, no. 22, pp. 8197–8203, 2005.

A. Sarafraz-Yazdi, A. Amiri, G. Rounaghi, and H. E. Hosseini, “A novel solid-phase microextraction using coated fiber based sol-gel technique using poly(ethylene glycol) grafted multi-walled carbon nanotubes for determination of benzene, toluene, ethylbenzene and o-xylene in water samples with gas chromatography-flam ionization detector,” Journal of Chromatography A, vol. 1218, no. 34, pp. 5757–5764, 2011.

B. Han, S. Ma, H. A. Aisa, X. C. Wang, and W. Chen, “Characterization andbiocompatibility of pegylated single walled carbon nanotubes,” Chinese Journal of Hospital Pharmacy, vol. 32, no. 18, pp. 1415–1419, 2012.

R. Zeineldin, M. Al-Haik, and L. G. Hudson, “Role of polyethylene glycol integrity in specific receptor targeting of carbon nanotubes to cancer cells,” Nano Letters, vol. 9, no. 2, pp. 751–757, 2009.

R. Singh, D. Pantarotto, L. Lacerda et al., “Tissue biodistribution and blood clearance rates of intravenously administered carbon nanotube radiotracers,” Proceedings of the National Academy of Sciences of the United States of America, vol. 103, no. 9, pp. 3357–3362, 2006.

L. Lacerda, A. Soundararajan, R. Singh et al., “Dynamic imaging of functionalized multi-walled carbon nanotube systemic circulation and urinary excretion,” Advanced Materials, vol. 20, no. 2, pp. 225–230, 2008.