Journal of Nanomaterials
Volume 2015 (2015), Article ID 872035, 9 pages
http://dx.doi.org/10.1155/2015/872035

Research Article

Functionalization of Hollow Mesoporous Silica Nanoparticles for Improved 5-FU Loading

Xiaodong She, Lijue Chen, Chengpeng Li, Canzhong He, Li He, and Lingxue Kong

Institute for Frontier Materials, Deakin University, Waurn Ponds, VIC 3216, Australia

Received 4 December 2014; Revised 26 February 2015; Accepted 5 March 2015

Academic Editor: Andrea Falqui

Copyright © 2015 Xiaodong She et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract.

Hollow mesoporous silica nanoparticles were successfully fabricated and functionalized with appropriate silanes. After modifications, amine, carboxyl, cyano, and methyl groups were grafted onto the nanoparticles and all functionalized hollow mesoporous silica nanoparticles maintained a spherical and hollow structure with a mean diameter of ~120 nm and a shell thickness of ~10 nm. The loading capacity of the hollow mesoporous silica nanoaprticles to the anticancer drug, 5-fluorouracil, can be controlled via precise functionalization. The presence of amine groups on the surface of nanoparticles resulted in the highest loading capacity of 28.89%, due to the amine functionalized nanoparticles having a similar hydrophilicity but reverse charge to the drug. In addition, the change in pH leads to the variation of the intensity of electrostatic force between nanoparticles and the drug, which finally affects the loading capacity of amine functionalized hollow mesoporous silica nanoparticles to some extent. Higher drug loading was observed at pH of 7.4 and 8.5 as 5-fluorouracil becomes more deprotonated in alkaline conditions. The improved drug loading capacity by amine functionalized hollow mesoporous silica nanoparticles has demonstrated that they can become potential intracellular 5-fluorouracil delivery vehicles for cancers.

1. Introduction

Nanoparticle based targeted drug delivery systems hold great promise for cancer therapy because of their targeting functions [1], sustained drug release profiles [2], reduced side effects [3], and the ability to overcome multidrug resistance (MDR) [2, 4]. A variety of materials have been exploited to meet different therapeutic needs, many with great potential for topical delivery [5–7]. When compared to polymer and carbon nanotubes which have been widely investigated as carriers in drug delivery systems [8–11], mesoporous silica nanoparticles (MSNs) have the potential to serve as a versatile drug nanocarrier for smart drug delivery [12]. For example, MSNs can level off the drug concentration at the targeted area as the drug molecules are released from the ordered pores by a tunable diffusion. This will reduce drug dosage and side effects [13]. Furthermore, MSNs are biocompatible, providing additional advantages for MSNs to be used as a platform for cancer treatment [14]. Choi et al. functionalized MSNs for the purpose of controlling drug release and developed a drug delivery system using MSNs as carriers based on the host-guest concept [15]. Mamaeva and coworkers [16] introduced a MSNs platform for cancer treatments. In their study, MSNs based drug delivery system can block the Notch signaling in cancer by controlling the delivery of γ-secretase inhibitors to cancer cells. Zhao et al. constructed a drug delivery system with sustained-release property based on rattle-type hollow magnetic mesoporous spheres and achieved an ibuprofen loading capacity of 302 mg g⁻¹ [17].

However, even though with a high surface area and a high pore volume, MSNs showed a relative low loading capacity of 5-fluorouracil (5-FU), a standalone therapeutic for the treatment of colon cancer, which may be due to the electrostatic repulsion between the drug and the carriers. In this case, in comparison to those carriers with a higher drug loading capacity, more MSNs were required to be taken up by cancer cells to achieve an effective cure concentration for cancer therapy. From a technical point of view, it is more preferable to deliver a smaller quantity of MSNs to minimise the side effects to patients [18]. Therefore, increasing the drug loading capacity of MSNs is essential for further optimizing the MSN based 5-FU delivery system.

The capacity to encapsulate drugs in nanoparticles can be improved by functionalizing or optimizing the drug encapsulation conditions [19]. Precisely controlling the grafting of chemical groups on MSNs would result in an enhancement in drug loading. For example, Sevimli and Yilmaz grafted primary amine, organothiol, and methyl groups onto MSNs via different alkoxysilanes and found that the amount of amoxicillin loaded was depended on the type of grafted functional groups [20]. Tomoiaga and coworkers functionalized magnetic MSNs with amino groups and achieved improved 5-FU loading capacity when compared to plain MSNs [21]. On the other hand, hollow MSNs (HMSNs) were reported to encapsulate more drugs than nonhollow ones as the hollow cavity provided more space for the storage of the drugs [22–24].

In this paper, HMSNs were modified for enhanced 5-FU loading. The partition coefficient of 5-FU was investigated to provide information about the degree of the hydrophilicity of 5-FU, which is crucial for selecting chemical groups for the subsequent functionalization. Amine, carboxyl, methyl, and cyano groups were introduced onto HMSNs. The structure of plain and functionalized HMSNs was characterized and the 5-FU loading capacity of HMSNs with and without chemical groups was investigated and compared.

2. Materials and Methods

2.1. Materials

Tetraethyl orthosilicate (TEOS, Si(OCH₂CH₃)₄), Triton X-100 (TX100), sulphuric acid, octadecyltrimethoxysilane (OTMS), (3-aminopropyl)triethoxysilane (APTES), 3-cyanopropyltriethoxysilane (CPTES), and fluorinated pyrimidine 5-fluorouracil (5-FU) were all purchased from Sigma Aldrich (Sydney, Australia). Eudragit S100 which is made in Germany was purchased from Shenzhen Youpuhui Pharmaceutical Co. Ltd (Shenzhen, China).

2.2. Methods and Characterizations

For the formation of the mesoporous silica shell, the Eudragit S-100 nanoparticle solution was ultrasonically dispersed for 3 min, followed by addition of 1.0 g TX100 under stirring. After TX100 was completely dissolved, 2.3 mL TEOS was added and this solution was left to proceed for 24 h before being transferred into a sealed container and hydrothermally treated overnight under 100°C. The resulting nanoparticles (as-synthesized HMSNs) were collected by centrifugation and redispersed in acetone for the removal of Eudragit core and TX100 templates. The particles were then washed 3 times with ethanol and deionized water, respectively, before finally freeze-drying.

For amine functionalization (HMSN-NH₂), 50 mg HMSNs were ultrasonically dispersed in 50 mL ethanol; 25 μL APTES was added and stirred for 24 h before centrifugations and washed 3 times with ethanol and then deionized water, respectively. Finally these yielded HMSN-NH₂ nanoparticles were collected and freeze-dried. The experimental procedure for methyl- and cyanofunctionalization (HMSN-CH₃ and HMSN-CN) is similar to amine functionalization. The only difference is to use OTMS and CPTES instead of APTES for chemical modification.

To prepare carboxyl-modified MSNs (HMSN-COOH), fifty milligrams of HMSNs was ultrasonically dispersed in 50 mL ethanol; 25 μL CPTES was added and stirred for 24 h. The resulting nanoparticles were collected by centrifugations and redispersed in sulphuric acid (50% v/v) and heated at 150°C for 4 h. The final products were recollected, washed, and freeze-dried.

Measurement of 5-FU oil : water partition coefficients was conducted via shake flash method according to OECD guideline 107 [25]. Firstly, n-octanol and Milli-Q water were saturated, respectively, prior to the experiments and herein their saturated solutions were referred to as octanol and water. Then, 5-FU was dissolved in water to obtain a concentration of 266.7 μg/mL. A combination of octanol and water with an octanol : water ratio of 1 : 1, 1 : 2, and 2 : 1 was added to centrifuge tubes and a total volume of 40 mL was maintained. Each ratio was carried out in triplicate. 1 mL 5-FU was added to the centrifuge tubes and the phases were mixed by vortex for 10 min before centrifuging at 1000 rpm for a further 10 min. Both 5-FU concentrations in water and octanol phases were determined via UV-Vis spectroscopy (Ocean Optics system, USA). The partition coefficient is calculated from the data of each run using the following equation:

The loading of 5-FU was carried out by soaking HMSNs in a concentrated drug solution at room temperature for 24 h while stirring to ensure the diffusion of the drug molecules through the mesopores. Firstly, 5-FU was dissolved in deionized water to a concentration of 3 mg/mL. 150 mg of HMSNs was ultrasonically dispersed in 50 mL of the 5-FU solution. The mixture was stirred at room temperature. At timed intervals of 0, 2, 4, 6, 8, 12, and 24 h, 0.5 mL of the solution was extracted and centrifuged to collect the supernatant for UV-Vis analysis at a wavelength of 266 nm while the 5-FU-loaded HMSNs precipitate was placed back into the drug solution. The 5-FU loading in the HMSNs was calculated by subtracting the amount of 5-FU in the supernatant from the amount in the original drug loading solution. The drug loading capacity was calculated using the following equation:

The morphology of HMSNs was studied using scanning electron microscopy (SEM, Carl Zeiss AG SEM Supra 55VP) and transmission electron microscopy (TEM, LaB6 JEOL JEM-2100). Before imaging under SEM, all the samples were coated with carbon through a BAL-TEC SCD 050 sputter coater (Leica Microsystems, Australia). TEM imaging was conducted under 200 kV. Nitrogen absorption and desorption isotherms of HMSNs were conducted on a Micromeritics Tristar 3000 (Particle & Surface Science, UK) equipment at 77 K. Before measurements, samples were degassed at 523 K for 1 h under nitrogen. The specific surface areas of HMSNs were calculated using the Brunauer-Emmett-Teller (BET) method. The Barrett-Joyner-Halenda (BJH) model was utilized to obtain the pore size distributions from the desorption branch of isotherms. Thermogravimetric analysis (TGA) of HMSNs was conducted using a TG Simultaneous Thermal Analyser (STA 449C, ZETZSCH, Germany). Samples with a weight of ~5 mg were placed into aluminium oxide crucibles and heated under nitrogen condition at a heating rate of 10°C/min from room temperature to 600°C. Fourier transform infrared (FTIR) spectra were measured with a Vertex 70 spectrometer (Bruker, Germany). All the samples were compressed into KBr pellets and recorded at 64 scans from 4000 cm⁻¹ to 400 cm⁻¹ with a resolution of 4 cm⁻¹.

3. Results and Discussions

3.1. Partition Coefficient of 5-FU

The partition coefficient () is defined as the quotient of two concentrations of a test substance (like 5-FU) in a two-phase system, where two largely immiscible solvents (n-octanol and water) were mixed with a fixed volume ratio. The logarithm of between n-octanol and water () has often been used as a key parameter to describe the lipophilicity of a molecule. In the pharmaceutical field, is utilized to estimate the biological activities of the drugs, such as solubility [26], biodegradation rate, bioaccumulation, membrane permeability [27], and drug absorption and toxicity predictions. Therefore, the investigation of the of 5-FU is essential to predict its transport activity, thereby providing information for the subsequent modification of HMSNs specific for high 5-FU loading.

The shake flask method is one of the common and standard experimental procedures adopted for study. This method is applied to determine the solubility and hydrophobicity of compounds with values ranging from −2 to 4. The determination is based on the principle that hydrophobic substances soluble in the lipid phase show a positive (), while negative values () typify polar compounds soluble in the water phase.

According to OECD guideline 107 [25], an effective partition coefficient study refers to all values obtained falling within a range of −2~4 with an offset of ±0.3 units. As shown in Table 1, the values of 5-FU for all ratios (octanol : water) are within the range and are consistently negative, indicating that 5-FU is hydrophilic. Specifically, when the volume of water decreased from 26.67 mL to 13.33 mL, the 5-FU concentration in water phase increased significantly from 7.747 μg/mL to 13.17 μg/mL. This is due to the fact that most 5-FU molecules prefer congregating in the water phase; thus, with a decrease in the water volume, a more concentrated 5-FU solution is observed. In contrast, no obvious difference was found in 5-FU concentration in the octanol phase. With varying octanol volume, from 26.67 mL to 13.33 mL, 5-FU concentration remained at around 2.2~2.6 μg/mL, which can be explained by the hydrophilic property of 5-FU.

Table 1: Partition coefficient of 5-FU and relevant parameters.

1 : 2 (O : W)
1 : 1 (O : W)
2 : 1 (O : W)

Water volume (mL)
26.67
20
13.33

Oil volume (mL)
13.33
20
26.67

(g/mL)
7.747
9.925
13.17

(g/mL)
2.279
2.380
2.629

0.2942
0.2398
0.1996

−0.5314
−0.6201
−0.6997

The weight percentages of 5-FU dissolved in each phase with varying octanol to water ratio were calculated. As seen in Figure 1, even though the volume of water and octanol changes, it appears that, in all ratios, around 75% of 5-FU is dissolved in the water phase while the other ~25% was found in the octanol phase. Therefore, a more hydrophilic surface of HMSNs would increase the loading of 5-FU.

Figure 1: Percentages of 5-FU dissolved in each phase with varying octanol to water ratio.

3.2. Modification of HMSNs

Surface-functionalized HMSNs were synthesized by postgrafting method where chemical groups were introduced to the surface of as-synthesized HMSNs using appropriate silanes. CPTES, APTES, and TEMS were used to prepare HMSN-CN, HMSN-NH₂, and HMSN-CH₃ while HMSN-COOH was obtained by oxidizing HMSN-CN. In all cases, plain HMSNs without any modification were used as a control. The morphology of modified and plain HMSNs was characterized by TEM. As shown in Figure 2, all modified HMSNs (Figures 2(b)–2(e)) had a similar morphology to that of plain HMSNs (Figure 2(a)) revealing that the structure of these nanoparticles can be well preserved during all functionalization processes. In particular, the surface-functionalized HMSNs maintained a hollow structure and a very spherical shape with a mean diameter of ~120 nm and a shell thickness of ~10 nm. In addition, all HMSNs displayed a highly uniform size, shell thickness, and, more importantly, a good dispersion which is one of the main requirements for drug delivery systems.

(a)

(b)

(c)

(d)

(e)

Figure 2: TEM images of (a) HMSNs; (b) HMSN-NH₂; (c) HMSN-COOH; (d) HMSN-CN; and (e) HMSN-CH₃.

The presence of mesoporous structure in all HMSNs was confirmed by BET (Figure 3). The nitrogen adsorption/desorption experiments demonstrated that both modified and plain HMSNs showed characteristic isotherms of mesoporous materials, a type IV isotherm, indicating the existence of mesopores [17, 28, 29]. Well-defined steps which occurred in the relative pressure of 0.35–0.55 and 0.9–1.0 were related to capillary condensation and desorption in open mesopores and interstitial pores, respectively [30]. As seen in Figure 3(b), narrow pore size distributions are presented in HMSNs before and after surface modifications. The average pore size of all HMSNs was calculated using the desorption branch of the isotherm by the typical Barrett-Joyner-Halenda (BJH) method, according to which HMSNs, HMSN-NH₂, HMSN-CN, HMSN-COOH, and HMSN-CH₃ have a pore size of 2.78 nm, 2.47 nm, 2.28 nm, 2.61 nm, and 2.51 nm, respectively.

(a)

(b)

Figure 3: N₂ adsorption-desorption isotherms (a) and the corresponding pore size distributions (b) of functionalized and plain HMSNs.

Structural parameters of the HMSNs with and without modifications were summarized in Table 2. No significant differences can be found in the pore size and the pore volume between nanoparticles functionalized with different groups, proving that surface functionalization had a limited effect on the morphology and microstructure of the final products. The maintaining of pore size and pore volume guarantees large spaces in functionalized HMSNs for drug loading that ideally should be same as nonfunctionalized ones. However, due to the coverage of functional groups, the surface area of functionalized samples decreased when compared to plain HMSNs. These results were consistent with similar studies [20].

Table 2: Structure parameters of functionalized and nonfunctionalized HMSNs.

HMSNs
HMSN-CH₃
HMSN-CN
HMSN-NH₂
HMSN-COOH
Plain HMSNs

Pore size (nm)
2.51
2.28
2.47
2.61
2.78

Pore volume (cm³/g)
0.43
0.50
0.43
0.47
0.53

Surface area (m²/g)
434
514
413
498
820

Different chemical groups of –NH₂, –CN, –COOH, and –CH₃ were grafted onto the surface of HMSNs separately and the successful grafting was demonstrated from FTIR spectra (Figure 4). All samples have strong bands located at around 460 (), 1100 (), and 3300–3500 cm⁻¹ (), confirming the presence of the SiO₂ inorganic phase [31]. The –NH₂ functionalization was clearly evidenced by the bands detected at around 1500 cm⁻¹ and 3000 cm⁻¹, which were unambiguously attributed to the NH bending vibration and C–N stretching vibration, respectively [20, 31–34]. Similarly, –CN and –COOH functionalizations were proved by the observation of relative characteristic peaks. The peak at 2200 cm⁻¹ corresponding to cyano groups (CN) was observed in the spectrum of HMSN-CN while HMSN-COOH showed a peak at 1730 cm⁻¹ relating to C=O vibration of COOH. The bands appearing at around 3000 cm⁻¹ in the spectrum of HMSN-CH₃ were assigned to C–H vibration of methyl groups (–CH₃), confirming the existence of –CH₃ on the surface of HMSN-CH₃. Results obtained by FTIR proved the effective surface functionalization of HMSNs, thereby confirming the presence of –NH₂, –CN, –COOH, and –CH₃ on the surface of HMSN-NH₂, HMSN-CN, HMSN-COOH, and HMSN-CH₃, respectively.

Figure 4: FTIR spectra of plain and functionalized HMSNs.

Thermogravimetric analysis (TGA) was carried out to further confirm the effective surface modification of functionalized HMSNs. All samples were tested under nitrogen condition at the temperature ranging from room temperature up to 650°C. The weight loss of samples obtained from TGA arose from the decomposition of chemical groups; thereby a difference in the weight loss between samples can indicate the relative amount of chemical groups grafted onto particles. As seen from Figure 5, the total weight loss of plain HMSNs was ~8.0% due to the dehydroxylation of silanol groups (Si–OH). In contrast, all functionalized HMSNs showed a total weight loss of ~20% which was mainly from thermal cracking of grafted functional groups. Therefore, the success of the grafting has been proven by TGA results, which was in agreement with the FTIR results illustrated above and the relative amount of functional groups of modified HMSNs was ~12% (w/w). Compared to other studies conducting functionalization on MSNs [20, 35], the weight loss caused by the functional groups reported here is slightly higher, suggesting the high efficiency of the current functionalization.

Figure 5: TGA curves of functionalized HMSNs and plain HMSNs.

3.3. 5-FU Loading Capacity of HMSNs with Different Chemical Modifications

The 5-FU loading capacities of functionalized and nonfunctionalized HMSNs have been monitored using a UV spectrophotometer. An initial drug concentration of 3 mg/mL was applied and the concentration change after 24 hours of loading was tracked. The loading capacity of all HMSNs were calculated and presented in Figure 6. For HMSNs, the internal macropore (the hollow cavity) is used to carry the loaded drug, while the mesopores in the shell are applied for drug storage as well as for transportation and controlled release of drugs from the macropore.

Figure 6: Loading capacities of functionalized and nonfunctionalized HMSNs.

5-FU was absorbed in both the pores and the hollow cavity and the external surface area. Firstly, 5-FU is attached on the surface of nanoparticles; as the loading process goes on, 5-FU can pass through the pores (2-3 nm) and get into the cavity (~100 nm). As the pores are much bigger than the drug molecules (~0.5 nm) and can provide enough room for the free transportation of the drug, the drug can be encapsulated into the pores and the cavity of HMSNs.

The presence of different functional groups on the external area of nanoparticles will affect the loading capacity of the nanoparticles. As expected, a difference in the amount of 5-FU loading after surface functionalization was observed, resulting from the different hydrophobicity and hydrophilicity of the functional groups [20]. A loading percentage of 18.34% was achieved for the plain HMSNs but increased to 28.89% after amine functionalization. It was reported that, by using amine functionalized mesoporous silica nanoparticles (MSNs, nonhollow structure) specifically for 5-FU encapsulation, a 5-FU loading of 124 mg 5-FU/g MSNs was achieved [21]. It is known that the degree and spatial distribution of the functionalities can affect the drug loading process. However, the hollow morphology is another parameter that could contribute to the high efficiency in drug loading of MSNs with a hollow morphology [22–24]. Therefore, this enhanced loading capacity (Table 3) might be attributed to the hollow structure of HMSNs and the functionalization.

Table 3: Loading capacities of functionalized and nonfunctionalized HMSNs.

HMSNs
HMSN-CH₃
HMSN-CN
HMSN-NH₂
HMSN-COOH
Plain HMSNs

Loading capacity (mg 5-FU/g HMSNs)
127.3
225.4
288.9
207.3
183.4

Furthermore, –CH₃ functionalization decreases the loading capacity of HMSNs to some extent (from 18.34% to 12.73%). 5-FU is a water-soluble drug; therefore, it is harder for it to approach HMSNs functionalized with methyl groups which are slightly more hydrophobic. On the other hand, –CN and –COOH functionalized HMSNs showed a moderate improvement in loading with a loading percentage of 22.54% and 20.73%, respectively. Although HMSN-COOH is hydrophilic which is similar to 5-FU, its loading capacity is inferior to HMSN-NH₂. This can be explained by the different surface charge of HMSN-NH₂ and HMSN-COOH. Given that 5-FU is negatively charged, it is much easier for 5-FU to get inside the positively charged HMSN-NH₂ via electrostatic attractions. However, the negative charge of –COOH groups may repel 5-FU molecules and decrease the 5-FU loading capacity of HMSN-COOH.

3.4. The Effect of pH on Drug Loading and Release from HMSN-NH₂

The –NH₂ on HMSNs can significantly increase the amount of 5-FU encapsulated into nanoparticles due to the similar hydrophilicity of the HMSN-NH to drug and the existence of positive charge provided by –NH₂ groups. Therefore, the control of the intensity of electrostatic force between nanoparticles and 5-FU may further regulate 5-FU loading capacity of particles.

In this regard, the loading of 5-FU by HMSN-NH₂ was carried out in 4 different pHs, 4.0, 5.5, 7.4, and 8.5 (Figure 7). As pH increases, the 5-FU loading capacity of HMSN-NH₂ increases and the maximum loading capacity of 27.52% is attained at pH of 8.5. HMSN-NH₂ is positively charged at a wider pH range because of the protonation of the amine groups while, under different pH values, the behaviour of 5-FU in terms of surface charge is different [36, 37]. As shown in Figure 8, the deprotonation of 5-FU can occur at either N1 or N3 or both to form the monoanions AN1 and AN3 and dianion under alkaline condition. It is reported that, at pH 7–10, AN1, AN3, and dianion of 5-FU coexist as a mixture in aqueous medium [36, 37]. Therefore, at acidic pHs (4.0 and 5.5), 5-FU becomes less deprotonated or nondeprotonated, which results in a very low surface charge of 5-FU. Therefore, lower drug loading capacity of HMSN-NH₂ (18.56% and 19.03%) was observed at these two pHs. Nevertheless, when pH increases to 7.4 and 8.5, the deprotonation of 5-FU enhances, thereby enhancing electrostatic attractions between HMSN-NH₂ and 5-FU. Instead of being a neutral molecule in the medium, most 5-FU molecules transfer to monoanions and dianion, which generates negative charges and leads to more 5-FU being encapsulated into the nanoparticles.

Figure 7: 5-FU loading capacity of HMSNs and HMSN-NH₂ at different pH values.

Figure 8: 5-FU anions (AN1 and AN3) and dianion.

On the other hand, no significant differences can be seen in the loading capacity of HMSNs at various pHs (Figure 8). Due to the presence of negatively charged silanolate groups on the surface, the loading capacity of HMSNs was inferior to HMSN-NH₂ at all pHs. The encapsulation of 5-FU by HMSNs might be mainly based on hydrogen bonding rather than electrostatic force. In addition, under alkaline pHs where 5-FU possesses more negative charges, a small decrease in drug loading capacity of HMSNs was found. This could be due to the repulsive interaction between carriers and the drug.

4. Conclusion

HMSNs were successfully functionalized with appropriate silanes. After functionalization, HMSNs with different chemical groups on the surface maintained the intact hollow structure and no significant changes were found with the pore size and pore volume. However, due to the coverage of functional groups, the surface area of functionalized samples decreased when compared to plain HMSNs, which were consistent with similar studies. The surface modification of functionalized HMSNs was proven by FTIR and TGA to be effective and the relative amount of grafted functional groups was ~12% (w/w). The loading capacity of HMSNs specific to 5-FU can be improved via precise functionalization. The presence of –NH₂ groups on the surface of nanoparticles resulted in the highest 5-FU loading capacity of 28.89%. The similar hydrophilicity of the HMSN-NH₂ and the drug and the presence of reverse charge of the drug give rise to this highest loading capacity of HMSN-NH₂. In addition, when compared to that of nonhollow MSNs reported in a very similar study, the loading amount of 5-FU by HMSN-NH₂ was much higher, suggesting that, besides functionalization, 5-FU loading could be improved by designing and fabricating MSNs of different structure such as the hollow MSNs. The change in pH leads to the variation of the intensity of electrostatic force between nanoparticles and 5-FU, which finally affect the loading capacity of HMSN-NH₂ to some extent. Higher drug loading of HMSN-NH₂ was observed at pH of 7.4 and 8.5 due to the fact that 5-FU becomes more deprotonated in alkaline conditions. By incorporating targeting molecules such as folic acid and epidermal growth factor (EGF), HMSN-NH₂ with improved loading of 5-FU will definitely render the current drug delivery system more versatile for applications in cancer therapy.

Conflict of Interests

The authors declare that there is no conflict of interests regarding the publication of this paper.

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